Digital Commons Network^™

Modeling Pichia Pastoris Growth On Methanol And Optimizing The Production Of A Recombinant Protein, The Heavy-Chain Fragment C Of Botulinum Neurotoxin, Serotype A, Wenhui Zhang, Mark A. Bevins, Bradley A. Plantz, Leonard A. Smith, Michael M. Meagher

Papers in Biotechnology

An unstructured growth model for the recombinant methylotrophic yeast P. pastoris Mut+ expressing the heavy-chain fragment C of botulinum neurotoxin serotype A [BoNT/A(Hc)], was successfully established in quasi-steady state fed-batch fermentations with varying cell densities. The model describes the relationships between specific growth rate and methanol concentration, and the relationships between specific methanol and ammonium consump-tion rates and specific growth rate under methanol-limited growth conditions. The maximum specific growth rate (μ) determined from the model was 0.08 h−1 at a methanol concentration of 3.65 g/L, while the actual maximum μ was 0.0709 h−1. The maximum specific methanol consumption rate was …

Improving Drought Early Warning Systems In The Context Of Drought Preparedness And Mitigation, Donald A. Wilhite, M. V. K. Sivakumar, Deborah Wood

Drought Network News (1994–2001)

Effective drought early warning systems are an integral part of efforts worldwide to improve drought preparedness. Timely and reliable data and information must be the cornerstone of effective drought policies and plans. In pursuit of the goal of improving the effectiveness of drought early warning systems, participants of the experts meeting were asked to address three fundamental questions:

1. What is your assessment of the current status of drought early warning systems?
2. What are the shortcomings, limitations, and needs for drought early warning systems?
3. How can drought early warning systems be improved to better support drought preparedness and …

Textile Society Of America Newsletter 12:3 – Fall 2000, Textile Society Of America

Textile Society of America: Newsletters

Learning to Weave in Ghana by Emilia Karr
TSA Symposium 2000 Activities
Textile Study at UMass
Members Honored
Letter from the President
TSA News
Conference Reviews
Exhibition Reviews
New Members
Fellowships
Calls for Papers
Conferences
Exhibitions Calendar
Tours/Courses
Lectures/Workshops
Publications
Virtual Textiles
Membership Application
TSA Calendar

Nordinfo: Research And Academic Library Cooperation Across National Borders, Sigrún Klara Hannesdóttir

E-JASL: Electronic Journal of Academic and Special Librarianship (1999-2009, Volumes 1-10)

NORDINFO, the Nordic Council for Scientific Information was founded as a bridge between research libraries and the growing information and documentation sector. NORDINFO’s secretariat was set up in Helsinki, Finland, and some permanent staff 6 was appointed to coordinate the activities. The full name behind the acronym was “The Nordic Cooperative Council for Research Libraries and Scientific Information and Documentation.”

When looking at the history of Nordic research library cooperation, it is clear that the members of the committee that suggested the establishment of an institution that would combine librarianship, documentation and information were people that understood what developments were …

The Americans With Disabilities Act And Academic Libraries In The Southeastern United States, Linda Lou Wiler, Eleanor Lomax

E-JASL: Electronic Journal of Academic and Special Librarianship (1999-2009, Volumes 1-10)

Individuals with disabilities are one of the fastest-growing segments of United States society. In 1970, 11.7% of the United States population was limited in activity, a major factor in measuring and identifying people with disabilities. In 1990, because of the aging of America, 13.7 % of the population could be so identified. By 1994, 15% of the population fell into this group. During this latter period, the older population stayed fairly stable but children and younger adults with disabilities increased greatly. Many different figures, depending upon the method of counting, e.g., age groups included, or whether residence was in a …

A Survey Of Four Libraries In Kunming: Library Automation And Modernization In A Far Removed Province In China, Jianli Li, Mary Francis Marx

E-JASL: Electronic Journal of Academic and Special Librarianship (1999-2009, Volumes 1-10)

As of 2000, over the last fifteen years, in a major effort at modernization and with an increasing investment by the Chinese government, China established more than twenty national information systems. The libraries we visited were member of some of those national information systems. These national information systems have been charged with setting up the basic foundations of an information industry. They belong to, respectively, the Education System, the Cultural Ministry, the Science and Technology Committee System, and the Chinese Academy of Sciences.

Undergraduate Full Text Databases: Bell And Howell Medical Complete And Infotrac Health Reference Center-Academic, Lutishoor Salisbury, Bryan Davidson, Alberta Bailey

E-JASL: Electronic Journal of Academic and Special Librarianship (1999-2009, Volumes 1-10)

This study compares and contrasts InfoTrac and ProQuest primarily as full-text resources to supplement retrieval of references contained in the CINAHL database. These databases are analyzed by examining their scope in terms of the number and types of serials covered within specific areas using Ulrich's subject headings. Another important aspect of this study relates to the effectiveness of the two search engines for end-user retrieval and collection development.

"Drainage, Drainage, Drainage" Creating Natural Disasters In Southeastern Nebraska, William Keith Guthrie

Great Plains Quarterly (through 2013)

In May 1950 the Little Nemaha River valley in the southeastern quadrant of Nebraska suffered a record-breaking flood. For a short time at the town of Syracuse, the Little Nemaha River, which drained a watershed of 218 square miles, had an estimated discharge of 225,000 cubic feet per second. This was larger than any flood recorded since 1928 on the Missouri River at Omaha, which drained a watershed of 322,000 square miles! During this storm and flood twenty-three people lost their lives, fourteen in the Little Nemaha Valley. As night came on, floodwaters swept a commercial bus off a highway …

Creative Approaches To Teaching Science In An Honors Setting, Ursula Shepherd

Journal of the National Collegiate Honors Council Online Archive

There are many reasons to teach science literacy in a University Honors Program. As our program director, Dr. Rosalie Otero, stated when asked why she has made such a strong commitment to incorporating the teaching of science into our program at the University of New Mexico:

It is difficult to envision how one will be able to live effectively in the twenty-first century without having achieved scientific literacy. While every educated person will certainly not be a scientist, every educated person must possess sufficient knowledge of the scientific method and of fundamental concepts of the natural sciences to make informed …

Review Of Celluloid Indians: Native Americans And Film By Jacquelyn Kilpatrick, Michael Hilger

Great Plains Quarterly (through 2013)

So far as I know, Jacquelyn Kilpatrick is the first person of American Indian heritage to write a book about the portrayal of Indians in film. Her special commitment to the American Indian community manifests itself in her careful analysis of American Indian historical issues in the chapters on film history and in her attention to parallel images in literature, especially literature by Native Americans. Complementing her unique perspective is a strong knowledge of contemporary film theory and criticism, which enables her to read selected films in ways that diverge from readings found in previous books on this topic.

Beginning …

The Role Of Research And Scholarship In Enhancing The Quality Of Life For Latinos On The Great Plains, Miguel A. Carranza, Gustavo Carlo, Maria Rosario De Guzman

Great Plains Research: A Journal of Natural and Social Sciences (through 2013)

The preamble to The Convention on the Rights of the Child, "recalls the basic principles of the United Nations and specific provisions of certain relevant human rights treaties and proclamations; reaffirms the fact that children, because of their vulnerability, need special care and protection; and places special emphasis on the primary caring and protective responsibilities of the family, the need for legal and other protection of the child before and after birth, the importance of respect for the cultural values of the child's community, and the vital role of international cooperation in achieving the realization of children's rights" (UNICEF 2001). …

Acuta Journal Of Telecommunications In Higher Education

ACUTA Journal

In This Issue

Technology Advances: The View from 10,000 Feet

WAP: Are You Ready for a Wireless World?

Virtual Private Networks: How They Can Work for Colleges and Universities

Network Security: How's Your Posture?

Software for Rent: Contact ASP

Voicing My IPinion

Institutional Excellence Award: Colorado Christian University

Columns

Interview

Book Review

Water Current, Volume 32, No. 5, October 2000

Water Current Newsletter

NU Researchers Track Endangered and Threatened Sturgeon in Platte River
From the Director: Promises and Challenges of Embarking on New Journeys and Looking in New Directions
Meet the Faculty: F. Edwin (Ed) Harvey; Steven D. (Steve) Comfort
Eleventh Platte River Basin Ecosystem Symposium to be Held in Kearney February 27-28
Water Sciences Laboratory Completes Creighton Groundwater Study
Utility Deregulation Could Have Unforeseen Impacts on Water Resources and Ecosystems
Irrigation Well Data Available on the Internet
Jess Named Associate Director of NU Conservation and Survey Division
Festival of Color 2000
Water News Briefs
Great Plains Natural Resources Journal
CSREES Grant to …

Review Of Crazy Horse By Larry Mcmurtry, Joseph C. Porter

Great Plains Quarterly (through 2013)

Our finest living novelist of the American West, Larry McMurtry, portrays one of the West's most notable historic figures in Crazy Horse. Two commemorations frame this readable survey biography: one, the Korczak Ziolkowski mountain sculpture of Crazy Horse; the other, a medallion struck by the government rewarding Little Big Man's apparent complicity in killing him. Who was this "American Sphinx, the loner who inspired the largest sculpture on Planet Earth?" McMurtry further asks what Crazy Horse meant "to his people in his lifetime, and also what he has come to mean to generations of Sioux in our own century …

Magnetic Properties Of Ni Nanowires In Self-Assembled Arrays, M. Zheng, L. Menon, H. Zeng, Yi Liu, Supriyo Bandyopadhyay, Roger D. Kirby, David J. Sellmyer

Department of Physics and Astronomy: Faculty Publications

Magnetic properties of Ni nanowires electrodeposited into self-assembled porous alumina arrays have been investigated. By anodizing aluminum in sulfuric acid and immersing the as-anodized template into phosphoric acid for different lengths of time, we are able to vary the diameters of the subsequently deposited nanowires between 8 and 25 nm. The coercivity measured along wire axis first increases with the wire diameter, reaches a maximum of 950 Oe near a diameter of 18 nm, and then decreases with further increase of wire diameter. The dependence of the magnetization of Ni nanowires is found to follow Bloch’s law at low temperature …

Two Large-Insert Soybean Genomic Libraries Constructed In A Binary Vector: Applications In Chromosome Walking And Genome Wide Physical Mapping, K. Meksem, K. Zobrist, E. Ruben, D. L. Hyten, T. Quanzhou, H-B. Zhang, D. A. Lightfoot

Department of Agronomy and Horticulture: Faculty Publications

Large DNA insert libraries in binary T-DNA vectors can assist in the isolation of the gene(s) under-lying a quantitative trait locus (QTL). Binary vectors facilitate the transfer of large-insert DNA fragments containing a QTL from E. coli to Agrobacterium sp. and then to plants. We constructed two soybean large-insert libraries from cv. Forrest in the pCLD04541 (V41) binary vector after partial digestion of genomic high-molecular-weight DNA with BamHI or HindIII. The libraries contain 76,800 clones with an average insert size of 125 kb, and therefore represent 9.5-fold haploid genome equivalents. Colony hybridization using a chloroplast-specific probe infers that …

Teaching In A Hands-On Environment - Outline, Tracy Bicknell-Holmes

University of Nebraska-Lincoln Libraries: Presentations

No abstract provided.

Analysis Of The Nico2O4 Spinel Surface With Auger And X-Ray Photoelectron Spectroscopy, J.-G. Kim, D. L. Pugmire, D. Battaglia, Marjorie Langell

Marjorie A. Langell Publications

Nickel cobaltite, NiCo₂O₄, has been synthesized by sol–gel and thermal decomposition techniques and the surface composition studied with Auger (AES) and X-ray photoelectron spectroscopies (XPS). The asintroduced samples are near-stoichiometric, although samples fabricated by thermal decomposition tend to be oxygen-defi cient by approximately 25% of the predicted spinel concentration. Nickel 2p XPS indicates the predominant form of the metal to be Ni²⁺, with the cations located in octahedral sites. The cobalt cations are equally divided between tetrahedral and octahedral sites as Co³⁺. The oxygen XP 1s spectrum is composed of two peaks, …

Fish & Wildlife News: September/October 2000

Fish and Wildlife News

Contents:
Program, Get Your FWS Program Here 2
Condors Dead; Lead Poisoning Suspected 6
Paradise Rises from Waste 7
Special Section: Wildland Fire 8
Invasive Nightmare 12
Check Out these New Products 14
Landmark Prosecutions Cap Bird Case 15
Mining for Partnership Gold 16
Invasive Nightmare, The Sequel 17
Rare Parrots Fly Free 20
Planes, Cranes and Hospitals 22
Fish and Wildlife...In Brief 27

Dielectric Tensor For Interfaces And Individual Layers In Magnetic Multilayer Structures, Xiang Gao, Michael J. Devries, Daniel W. Thompson, John A. Woollam

Department of Electrical and Computer Engineering: Faculty Publications

The magneto-optical Kerr response of metallic magnetic multilayers has been studied by determining the dielectric tensors (dielectric functions) for individual layers, including the magnetic and nonmagnetic interfacial layers. The diagonal components of these tensors were determined using in situ ellipsometric analysis, where the ellipsometric data were taken in real time during multilayer deposition. The off-diagonal components were determined by regression fitting magneto-optic polar Kerr rotation and ellipticity data to models supported by electromagnetic theory. The Voigt parameters (ratio between off-diagonal and diagonal components of dielectric tensors) were determined from these model fits. Higher magnitudes for the Voigt parameters were found …

Nebline, September 2000

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

National 4-H Week: Volunteers Make A Difference
Growing Hardy Bulbs
Fall Storage of Tender Bulbs, Corms, and Tubers
Drying Gourds
Dealing with Yellowjackets
Is Your Home a Hide-Away for Pests?
Is There a Cancer Epidemic in the United States?
Be Prepared for Head Lice
Prepare Grain Bins and
Equipment Before Harvest
Sobering Safety Statistics
Could Carbon be Your Next Crop? USDA publication examines the issue
Are you Looking for the Latest Wheat Variety Test Results?
U.S. Drought Monitor
The Contingency Plan and the Exit Strategy
Benefits of Acreage Windbreaks
African Violet Growing Tips
Trees from Seeds
Teens Gain Confidence in …

The Association For Documentary Editing Annual Meeting, October 5-7, 2000, Berkeley, California

Documentary Editing: Journal of the Association for Documentary Editing (1979–2011)

The ADE'S annual meeting will be held Thursday to Saturday, October 5-7, in Berkeley, California. The registration fee for forms postmarked after 8 September and for those registering at the convention in Berkeley is $55.

Conference program

Fermentation Strategies For Recombinant Protein Expression In The Methylotrophic Yeast Pichia Pastoris, Wenhui Zhang, Mehmet Inan, Michael M. Meagher

Papers in Biochemical Engineering

Fermentation strategies for recombinant protein production in Pichia pastoris have been investigated and are reviewed here. Characteristics of the expression system, such as phenotypes and carbon utilization, are summarized. Recently reported results such as growth model establishment, application of a methanol sensor, optimization of substrate feeding strategy, DOstat controller design, mixed feed technology, and perfusion and continuous culture are discussed in detail.

Pseudomonas Syringae Hrp Type Iii Secretion System And Effector Proteins, Alan Collmer, Jorge L. Badel, Amy O. Charkowski, Wen-Ling Deng, Derrick E. Fouts, Adela R. Ramos, Amos H. Rehm, Deborah M. Anderson, Olaf Schneewind, Karin V. Van Dijk, James R. Alfano

Center for Plant Science Innovation: Faculty Publications

Pseudomonas syringae is a member of an important group of Gram-negative bacterial pathogens of plants and animals that depend on a type III secretion system to inject virulence effector proteins into host cells. In P. syringae, hrpyhrc genes encode the Hrp (type III secretion) system, and avirulence (avr) and Hrpdependent outer protein (hop) genes encode effector proteins. The hrpyhrc genes of P. syringae pv syringae 61, P. syringae pv syringae B728a, and P. syringae pv tomato DC3000 are flanked by an exchangeable effector locus and …

Theory Of Magnetostatic Coupling In Thin-Film Rectangular Magnetic Elements, Evgeny Y. Tsymbal

Evgeny Tsymbal Publications

A theory of magnetostatic coupling in thin-film uniformly magnetized arrays of rectangular magnetic elements is presented. Analytic expressions for the magnetostatic energy and the dipolar fields are derived. The influence of the aspect ratio and the spacing between the elements on the magnetostatic coupling is investigated. It is found that increasing the aspect ratio reduces the critical distance between the elements, above which the magnetostatic inter-element coupling can be neglected.

Flea News 60 (July 2000), Robert E. Lewis

Flea News

Announcement: Printed copies of Flea News discontinuing

Obituary: Franciscus Gerardus Albertus Maria Smit (June 7, 1920-January 28, 2000)

Book review: Sifonapteros do Brasil, Pedro Marcois Linardi and Lindolpho Rocha Guimaraes. Sao Paulo, Museu de Zoologia USP/FAPESP, 2000.

Flea species

Literature

Miscellanea: As of January 2000 the name of the Journal of the Helminthological Society of Washington was changed to Comparative Parasitology.

Acuta Journal Of Telecommunications In Higher Education

ACUTA Journal

In This Issue

Roundtable Discussion: Convergence: Dealing with Change

Convergence: A Framework for Change

Planning for Competition and Convergence

Voice and Telephony over IP: Risk Dimensions and Solutions

Cybervandals: Fending Off Attacks

Why Vendors See Colleges as a Key Market Segment

Campus Profile: Clarion University of Pennsylvania

Called to Serve: The Conscripted Consultant

Columns

Review Of Our Treasures/Nuestros Tesoros: A Celebration Of Nebraska's Mexican Heritage/Una Celebración De La Herencia Mexicana De Nebraska By Ralph F. Grajeda, Et Al, Juan R. García

Great Plains Quarterly (through 2013)

Our Treasures/Nuestros Tesoros is part of a grant funded project designed to collect and preserve the cultural heritage of Mexican Americans in Nebraska. The information presented is largely drawn from oral histories and interviews of residents living in Grand Island, Lincoln, Omaha, and Scottsbluff.

The published volume consists of five parts. "Mexicans in Nebraska," by Ralph Grajeda, provides a general survey of Mexican immigration into the state. Much of the information covers the period before Wodd War II and focuses on the first two waves of immigration of Mexicans into the US and Nebraska. Although those familiar with the history …

Canadian Cooperative Wildlife Health Centre Newsletter, Volume 7-1, Summer 2000

Canadian Cooperative Wildlife Health Centre: Newsletters and Publications

New Staff Member - Western/Northern Regional Centre Surveillance for West Nile Virus - Summer 2000 Dorsal-spined Larvae in Cervids Organophosphate Pesticide Poisoning of Seagulls - St. John's, Newfoundland Successful euthanasia of a juvenile fin whale Psittacosis in Wild Rock Doves Walleye myopathy The St-Lawrence beluga whale: summary of lesions detected in stranded individuals submitted for necropsy during 1999 An Epizootic of Iridovirus in Oregon Spotted Frog Tadpoles (Rana pretiosa) Epidemic Avian Cholera in Double-Crested Cormorants in Saskatchewan Winter Ticks in Stone's Sheep California Bighorn Sheep in British Columbia

American Society Of Parasitologists Newsletter, V. 22, No. 2, [Summer 2000], Scott Lyell Gardner

American Society of Parasitologists: Newsletter

An issue of the American Society of Parasitologists' quarterly newsletter, also called the Journal of Parasitology Newsletter.