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Genetic Engineering Of Livestock Improvement, Gordon E. Dickerson

World Congress on Genetics Applied to Livestock Production: 3rd (1986)

Genetic engineering requires clearly defined objectives, adequate genetic variability and effective methods of selection. In a given production-marketing System, relative importance of genetic change in alternative traits can be estimated from expected effects on cost per unit of output, using available biological and economic information. Diversity among existing breeds and their crosses is a primary and quickly usable genetic resource, and its management for future needs is a public concern. Better physiological and biochemical understanding of the genetic controls of animal development and function is important to permit more effective selection for efficiency of animal production under diverse environments. Long-term …

Columnaris Disease Of Fishes, G. L. Bullock, T. C. Hsu, E. B. Shotts Jr.

United States Fish and Wildlife Service: Publications

Columnaris disease is an acute to chronic bacterial infection that affects anadromous salmonids and virtually all species of warmwater fishes. Davis (1922), who first described the disease, named it columnaris because the causal bacterial cells seen in wet mounts of affected gills and fins were arranged in columnar aggregations. Ordal and Rucker (1944) were the first to isolate the causal organism and, based on cellular morphology, identified it as a myxobacterium. Organisms classified in the order Myxobacterales are long, thin gram-negative rods that are motile on agar media by a creeping or flexing motion. They have a life cycle composed …

G86-797 Causes Of Vaccination-Immunization Failures In Livestock, Duane Rice, E. Denis Erickson, Dale Grotelueschen

University of Nebraska-Lincoln Extension: Historical Materials

This NebGuide discusses reasons why vaccinations fail to provide immunity against disease, and how to prevent this from happening.

To comprehend the many reasons for vaccine failure, it is important to understand how animals and humans have the ability to resist infectious diseases. It is also important to know what a disease is and how it affects the animal.

According to Stedman's Dictionary, disease is an interruption, cessation or disorder of body functions, systems or organs. Diseases may be obvious even to the untrained eye, or detectable only by sophisticated testing procedures (subclinical disease). Serious irreversible damage may be …

Species-Specific Restriction Site Polymorphism In Root-Knot Nematode Mitochondrial Dna, Thomas O. Powers, E. G. Platzer, Bradley C. Hyman

Department of Plant Pathology: Faculty Publications

Research was initiated to physically characterize the mitochondrial genomes of several Meloidogyne spp. and host-races, to address questions regarding their systematics and dispersal, and to assess the possibility of developing molecular diagnostics for these nematodes. Techniques were developed for purification and rapid detection of mitochondrial DNA from root-knot nematodes. Mitochondrial DNAs among Meloidogyne spp. were demonstrated to exhibit extensive divergence. The potential for using the rapidly diverging mitochondrial genomes as a diagnostic assay for M. incognita, M. hapla, M. arenaria, and M. javanica is discussed.

Production Of Shiga-Like Toxin By Escherichia Coli, Lilian R. M. Marques, Marjorie A. Moore, Joy G. Wells, I. Kate Wachsmuth, Alison D. O'Brien

Uniformed Services University of the Health Sciences

Escherichia coli is one of several agents that cause intestinal disease in humans and animals. Four classes of E. coli have been recognized [1], Enterotoxigenic E. coli (ETEC) strains produce a heat-labile (LT) enterotoxin and/or a heat-stable( ST) enterotoxin. Enteroinvasive E . coli (EIEC) strains, like shigellae, penetrate and multiply within epithelial cells. Enteropathogenic E. coli (EPEC) strains belong to certain serogroups that have been incriminated as pathogens by epidemiological studies. Some EPEC strains have been shown to adhere to cells of the intestinal mucosa and to produce pathognomonic lesions at the site of attachment. Enterohemorrhagic E. coli (EHEC) strains …

Aging And Long-Term Care In Nebraska, James A. Thorson, Burce J. Horacek

Center for Public Affairs Research (UNO): Publications

A high proportion of Nebraska's population is age 65 or older and this is the fastest growing segment of the population. The population aged 85 years and older, which is the population most in need of long-term care, grew by over 45 percent between 1970 and 1980, and is expected to increased by 202 percent by the year 2000. This group includes individuals who are likely to require public assistance to help meet their medical and long-term health care needs. We estimate that the cost to the state to provide Medicaid assistance will increase from $60.4 million to 164.5 million …

Recovery Of Parasite Remains From Coprolites And Latrines: Aspects Of Paleoparasitological Technique, Karl J. Reinhard, Ulisses E. Confalonieri, Bernd Herrmann, Luiz F. Ferreira, Adauto J. G. De Araujo

Department of Anthropology: Faculty Publications

Standard techniques for the analysis of prehistoric soils have not been devised. It is unlikely that any single technique is applicable to all types of fecal remains. This is due to various environmental conditions which effect the preservation of helminth ova. In general, gravitational sedimentation is a useful technique for isolating helminth eggs and larvae from coprolites. Latrine soils pose greater problems for helminthological examination. Although various clinical techniques have been successfully utilized in soil study, it is important to remember that some latrine soils have not yielded helminth eggs to any clinical technique. Consequently the paleoparasitologist must be ready …

Formation Of Influenza Virus Particles Lacking Hemagglutinin On The Viral Envelope, Asit K. Pattnaik, Donald J. Brown, Debi P. Nayak

School of Veterinary and Biomedical Sciences: Faculty Publications

We investigated the intraceUular block in the transport of hemagglutinin (HA) and the role of HA in virus particle formation by using temperature-sensitive (Is) mutants (1s134 and 1s61S) of inOuenza virus AlWSN/33. We found that at the nonpermissive temperature (39.5°C), the exit of ts HA from the rough endoplasmic reticulum to the Golgi complex was blocked and that no additional block was apparent in either the exit from the Golgi complex or post-Golgi complex transport. When MDBK ceUs were infected with these mutant viruses, they produced noninfectious virus particles at 39.5°C. The efficiency of particle formation at 39.5°C was essentiaUy …

Small Mammal Community Structure And Composition In The Cerrado Province Of Central Brazil, Michael A. Mares, Kristina A. Ernest, Donald D. Gettinger

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Community structure, macrohabitat selection, and patterns of species co-occur- rence were examined during a 14-month study of small mammals in the Cerrado Province of central Brazil. Data were collected from mark-recapture grids in brejo and gallery forest, and from live-trapping and specimen collection in all habitat types within cerrado (campo limpo, campo sujo, cerrado [s.s.], cerradão, brejo, valley-side wet campo, and gallery forest). Gallery forest supported the highest species richness, most complex vertical distribution of species, highest level of trophic diversity, and highest macroniche diversity. Degree of habitat selection varied widely. All habitat types supported both rodents and marsupials, although …

Evolution And Historical Biogeography Of A Parasite-Host Assemblage: Alcataenia Spp. (Cyclophyllidea: Dilepididae) In Alcidae (Charadriiformes), Eric P. Hoberg

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The methodology of phylogenetic systematics was used to develop hypotheses for the evolution of eight species of Alcataenia, a group of host-specific cestodes of the Alcidae and, to a lesser extent, the Laridae (Charadriiformes). Concurrently, aspects of the early biogeography of alcids were reevaluated making it possible to study the historical and distributional relationships of parasites and hosts. The most parsimonious hypothesis for the phylogeny of Alcataenia suggested that sequential colonization or host switching with limited coevolution (coaccommodation) by parasites best explained the distributional patterns exhibited by Alcataenia spp. Morphological evolution of specific species of Alcataenia accompanied host switching, …

Polymorphism Of Eimerian Oocysts: A Dilemma Posed By Working With Some Naturally Infected Hosts, Brent B. Parker, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

First paragraph:

Morphological variation of sporulated oocysts within individual eimerian species is well documented (Joyner, 1982. In Biology of the Coccidia, P. L. Long (editor). University Park Press, Baltimore, pages 35-62). In some cases, oocysts of a single eimerian species are known to vary in size by as much as 40% during patency (Duszynski, 1971, Journal of Parasitology 57: 948- 952). During a survey to determine the prevalence of coccidiosis in sandhill cranes (Grus canadensis) wintering in New Mexico (Parker and Duszynski, 1986, Journal of Wildlife Diseases 21: 25-35), marked polymorphism was observed among sporulated oocysts of …

Aspects Of Ecology And Biogeography Of Acanthocephala On Antarctic Seabirds [Données Écologiques Et Biogéographiques Sur Les Acantocéphales D’Oiseaux Marins Antarctiques], Eric P. Hoberg

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Four species of acanthocephalans are reported from seabirds, nototheniid fishes and gammaridean amphipods in the western Antarctic. Corynosoma hamanni (von Linstow, 1892) was found in Phalacrocorax atriceps King, Chionis alba (Gmelin), Larus dominicanus Lichtenstein, and Catharacta lonnbergi (Mathews). The complete life cycle of this acanthocephalan was elucidated: cysticanths develop in the haemocoel of a gammaridean amphipod, Pontogeneiella sp. of the family Eusiridae and presumably, following ingestion of parasitized amphipods, encyst in the body cavity of Antarctic fishes of several species. Corynosoma singularis Skriabin and Nikol’skii, 1971 was found only in P. atriceps. Corynosoma bullosum (von Linstow, 1892) occurred in both …

Cysts Resembling Hydatids In A Chicken, Gerald D. Schmidt, Robert L. Rausch

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Two large cysts recovered from the abdominal cavity of a domestic chicken superficially resembled hydatid cysts of Echinococcus spp. No protoscolices were present. Microscopic examination of the internal lining of one of the cysts revealed a single cuboidal to columnar, ciliated epithelium, leading to a diagnosis of oviduct cysts. Earlier records of hydatids of fowl are reviewed.

Spanish abstract: Reporte de Caso--Quistes en un pollo semejantes a la hidatidosis. Se encontraron dos quistes grandes en la cavidad abdominal de un pollo doméstico. Superficialmente los quistes parecían quistes hidatidícos de Echinococcus spp. No se encontraron escólecis o cabezas de la tenia. …

Two New Species Of Litomosoides (Nematoda: Onchocercidae) From Pocket Gophers (Rodentia: Geomyidae) In Colorado, Scott Lyell Gardner, Gerald D. Schmidt

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Two new species of nematodes representing the genus Litomosoides were obtained from pocket gophers collected in the early 1960s and during 1982 in Colorado. Individuals of Litomosoides thomomydis n. sp. were recovered from the abdominal cavities of two species of pocket gopher, Thomomys tulpoides (Richardson) and T. bottae (Eydoux & Gervais). Nematodes representing L. westi n. sp. were recovered from the abdominal and pleural cavities of the plains pocket gopher Geomys bursarius (Shaw) from eastern Colorado. These two species of filarioid nematodes appear to be restricted in geographical range to those pocket gophers inhabiting the central grasslands and …

Eimeria Halleri Sp. N. (Apicomplexa: Eimeriidae) From The Round Stingray, Urolophus Halleri (Rajiformes: Dasyatidae), Steve Upton, John Bristol, Scott Lyell Gardner, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Eimeria halleri sp. n. (Apicomplexa: Eimeriidae) is described from the rectal contents ofthe round stingray, Urolophus halleri Cooper (Rajiformes: Dasyatidae) from Puerto Penasco, Mexico. Oocysts are spherical or subspherical, 16.9 x 16.8 (15.0-18.0 x 15.0-18.0) /μm, with a smooth, thin wall. Micropyle, polar granule, and oocyst residuum are absent. Sporocysts are ovoid, 11.1 x 6.8 (10.0-13.0 x 6.0-7.5) /μm and possess Stieda and substieda bodies. Sporozoites are comma-shaped, 9.9 x 3.2 (9.0-11.0 x 2.8-4.0) /μm and contain an ovoid posterior and a spherical anterior refractile body. The sporocyst residuum consists either of numerous finely granular particles scattered among the sporozoites …

Coccidiosis Of Sand Hill Cranes (Grus Canadensis) Wintering In New Mexico, Brent B. Parker, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The feces of 212 Sandhill cranes (Grus canadensis) collected in central New Mexico from October 1982 to January 1983 and from October 1983 to January 1984 were examined to determine the prevalence of coccidial oocysts. One hundred forty-five granulomatous nodules from the viscera of 64 cranes and samples of lung, small intestine, and large intestine from 58 birds were examined by light and transmission electron microscopy for the presence of intestinal or extraintestinal coccidiosis. Of the 212 fecal samples, 160 (75%) were positive for oocysts of Eimeria, including E. gruis in 139 (66%) and E. reichenowi in …

Eimerians From Different Karyotypes Of The Japanese Wood Mouse (Apodemus Spp.), With Descriptions Of Two New Species And A Redescription Of Eimeria Montgomeryae Lewis And Ball, 1983, Constance D. Wash, Donald W. Duszynski, Terry L. Yates

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Examination of 131 wood mice (Apodemus spp.) representing 2 species and 6 subspecies collected from the Japanese islands of Hokkaido, Honshu, Kyushu, and Tsushima showed that 70 mice (53%) had coccidian oocysts in their feces. These included 21 of 42 (50%) Apodemus argenteus argenteus; 7 of 14 (50%) Apodemus argenteus hokkaidi; 2 of 3 (67%) Apodemus argenteus sagax; 3 of 9 (33%) Apodemus speciosus ainu; 36 of 61 (59%) Apodemus speciosus speciosus; and 1 of 2 (50%) Apodemus speciosus tusimaensis. Four distinct coccidians were identified: Eimeria argenteus n. sp. from A. a. argenteus …

Phylogenetics And The Future Of Helminth Systematics, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Phylogenetic systematics is a relatively new formal technique that increases the precision with which one can make direct estimates of the history of phylogenetic descent. These estimates are made in the form of phylogenetic trees, or cladograms. Cladograms may be converted directly into classifications or they may be used to test various hypotheses about the evolutionary process. More than 20 phylogenetic analyses of helminth groups have been published already, and these have been used to investigate evolutionary questions in developmental biology, biogeography, speciation, coevolution, and evolutionary ecology.

American Society Of Parasitologists Henry Baldwin Ward Medal Acceptance Speech, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Transcript of American Society of Parasitologists Henry Baldwin Ward Medal acceptance speech, by Daniel R. Brooks, in 1985.

Eimeria Species (Apicomplexa: Eimeriidae) Infecting Peromyscus Rodents In The Southwestern United States And Northern Mexico With Description Of A New Species, David W. Reducker, Lynn Ann Hertel, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Of 198 deermice (Peromyscus spp.) collected from various localities in the southwestern United States and northern Mexico, 106 (54%) had eimerian oocysts in their feces when examined. These included 50 of 106 (47%) Peromyscus truei, 34 of 54 (63%) Peromyscus maniculatus, 4 of 17 (24%) Peromyscus leucopus, and 18 of 21 (86%) Peromyscus eremicus. The following Eimeria were identified from infected mice: Eimeria arizonensis and Eimeria langebarteli from P. truei; E. arizonensis, Eimeria peromysci, and Eimeria delicata from P. maniculatus; E. arizonensis and Eimeria lachrymalis n. sp. from P. eremicus; …

Synthesis And Localization Of A Development-Specific Protein In Sclerotia Of Sclerotinia Sclerotiorum, Geraldine Russo, James L. Van Etten

Department of Plant Pathology: Faculty Publications

A development-specific protein (SSP) makes up about 35 to 40% of the total protein in sclerotia of the fungus Sclerotinia sclerotiorum. The protein consists of three charge isomers, with one isomer making up 80 to 90% of the total. In vitro translation of poly(A)⁺ RNA isolated from cells in early stages of sclerotia formation revealed that 44% of the amino acids incorporated was into SSP. In vivo- and in vitro-synthesized forms of SSP migrated at identical rates on both isoelectric focusing and denaturing polyacrylamide gels, indicating that SSP was not synthesized as a larger precursor. This was significant …

Recovery Of Helminths From Prehistoric Feces: The Cultural Ecology Of Ancient Parasitism, Karl J. Reinhard

Karl Reinhard Publications

Several helminth parasites have been recovered from archaeological sites on the Colorado Plateau. These include Ascaris, Trichuris, Enterobius, Moniliformis, and tapeworms in the families Hymenolepidae and Taeniidae. The recovery of these animals demonstrates that cross-temporal: comparison of parasitism between different prehistoric lifestyles is possible. Such comparison might elucidate the effects of changing lifestyle and subsistance pattern on human parasitism.

Desiccated feces recovered from archaelogical deposits were studied. Hunter-gatherer feces were selected from Dust Devil Cave in southeastern Utah. Agriculturalist feces were studied from Antelope House, a cave site in northeastern Arizona, and Salmon Ruins, a Pueblo …

Helminth Parasites Of Thomomys Bulbivorus (Richardson) (Rodentia: Geomyidae), With The Description Of A New Species Of Hymenolepis (Cestoda), Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A cestode, Hymenolepis tualatinensis n. sp., is described from the pocket gopher, Thomomys bulbivorus (Richardson) (Rodentia: Geomyidae), from the Willamette Valley in Oregon. Helminths of four additional species were found. Trichuris fossor Hall, 1916, Ransomus rodentorum Hall, 1916, Heligmosomoides thomomyos Gardner and Jasmer, 1983, and Hymenolepis horrida (von Linstow, 1901), of which all but H. thomomyos represent new host records. A significant change in prevalence of the whipworm T. fossor in the population of T. bulbivorus from spring through summer was noted. Significant differences in prevalence of infection of helminths in pocket gophers collected from two different …

Coccidian Parasites (Apicomplexa: Eimeriidae) Of Microtus Spp. (Rodentia: Arvicolidae) From The United States, Mexico, And Japan, With Descriptions Of Five New Species, Tedman L. Vance, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Beginning in July 1980, 149 voles (Microtus spp.) representing 9 species and 14 subspecies collected in Japan, Mexico and the United States were examined for coccidia; 67 (45%) had oocysts in their feces. These included 1 of 3 (33%) M. californicus sactidiegi; 0 of 1 M. longicaudus longicaudus; 0 of 1 M. l. macrurus; 48 of 111 (43%) M. mexicanus including 11 of 26 (42%) M. m. fulviventer, 1 of 2 (50%) M. m. fundatus, 13 of 31 (42%) M. m. mexicanus, 1 of 4 (25%) M. m. mogollonensis and 22 of 48 …

Reticulotaenia N. Gen. For Lateriporus Australis Jones And Williams, 1967, And Lateriporus Mawsoni Prudhoe, 1969 (Cestoda: Dilepididae), From Sheathbills, Chionis Spp., In Antarctica, With A Consideration Of Infraspecific Variation And Speciation, Eric P. Hoberg

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Reticulotaenia n. gen. was established in the family Dilepididae for two species of cestodes, formerly referred to Lateriporus Fuhrmann, 1907, that are characteristic helminths in sheathbills, Chionis spp., in Antarctica. The new genus was most similar to Lateriporus and Dilepis Weinland, 1858, but was easily distinguished from these and other genera of Dilepidinae having a unilateral genital pore. The salient features of Reticulotaenia were a rostellum armed with a single row of 10 hooks, a vaginal sphincter, and a reticulate ovary and uterus. Reticulotaenia australis (Jones and Williams, 1967) n. comb. was redescribed on the basis of specimens from Chionis …

Eimeria From Bats Of The World: A New Species In Tomopeas Ravus From Peru, Donald W. Duszynski, Linda J. Barkley

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Two of 17 (12%) crevice bats, Tomopeas ravus (Chiroptera: Vespertilionidae), from Peru had coccidian oocysts in their feces when examined. Sporulated oocysts of Eimeria tomopea n. sp. are ellipsoid to subspheroid, 30.6 x 24.6 (26-34 x 20-28) μm with ovoid sporocysts 13.9 x 9.0 (12-15 x 8-10) μm. A micropyle and substieda body are absent, but polar bodies, oocyst and sporocyst residua, and small, indistinct Stieda bodies are present. The oocyst wall is thick, ≈1.5 μm and consists of 2 layers, the outermost being rough and mammillated. This is only the 13th eimerian to be described from bats worldwide. The …

Presidential Address: Parasitology: Retrospect And Prospect, Robert L. Rausch, Gerald D. Schmidt

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Transcript of the presidential address given by Dr. Robert L. Rausch, of the University of Washington, at the 59th Annual Meeting, American Society of Parasitologists, August 9, 1984, in Snowbird, Utah, with a transcript of introductory remarks given by Dr. Gerald D. Schmidt, of the University of Northern Colorado.

Ec85-198 Nebraska Poisonous Range Plants, Patrick E. Reece, Charles P. Moser

University of Nebraska-Lincoln Extension: Historical Materials

Extension Circular 85-198: Nebraska Poisonous Range Plants. This circular helps people identify plants that grow in Nebraska that may be poisonous.

Parasitology And Pathology Of Marine Organisms Of The World Ocean, William J. Hargis Jr.

United States Department of Commerce: Staff Publications

The Symposium in which the communications, as they were called during the meeting, comprising this volume were presented was held at the Zoological Institute of the Academy of Sciences of the U.S.S.R. in Leningrad during 13 to 16 October 1981. Conducted as part of the cooperative program of the U.S.A.-U.S.S.R. Working Group on Biological Productivity and Biochemistry of the World Ocean, the Leningrad meeting was sponsored by the Academy of Sciences of the U.S.S.R. (the Zoological Institute) and the Ministry of Fisheries of the U.S.S.R. (The Scientific Council on Fish Diseases of the Ichthyological Commission). It was an extremely interesting …

Sex Differences In The Recognition Of Infant Facial Expressions Of Emotion: The Primary Caretaker Hypothesis, Raymond B. Hames, Wayne A. Babchuk

Department of Anthropology: Faculty Publications

Although much research has been devoted to studying sex differences In functioning (e.g., Maccoby and Jacklin 1974), most efforts have been directed toward documenting or elucidating the proximate causes of sex differences. Few attempts have been made, however, to explain the ultimate causes of these differences or the selective pressures that have led to the development or psychological differences between males and females [for exception see Symons (1979) and Daly and Wilson (1983)]. Toward this end of blending psychology with evolutionary theory we develop what we call the " primary caretaker hypothesis," which predicts that the sex that through evolutionary …