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Lagarocotyle Salamandrae N. Gen., N. Sp. (Monogenoidea, Polyonchoinea, Lagarocotylidae N. Ord.) From The Cloaca Of Rhyacotriton Cascadae Good And Wake (Caudata, Rhyacotritonidae) In Washington State, Delane C. Kritsky, Eric P. Hoberg, K. B. Aubry

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Lagarocotyle salamandrae n. gen., n. sp. (Lagarocotylidea n. ord., Lagarocotylidae n. fam.) is described from the cloaca of the Cascade torrent salamander, Rhyacotriton cascadae Good and Wake (Rhyacotritonidae), from the Lewis River and Wind River drainages of south-central Washington. Lagarocotyle n. gen. is characterized, in part, by a haptor armed with 16 hooks (14 submarginal, two subcentral), blind intestinal ceca, a large testis surrounding the germarium, an open male copulatory organ (not tubular), a dextroventral vaginal pore (at level of copulatory complex), and an egg lacking filaments; eyes, head organs, seminal receptacle, and haptoral anchors and bars are lacking. Lagarocotyle …

Rapid Assessment Of Drug Susceptibilities Of Mycobacterium Tuberculosis By Means Of Luciferase Reporter Phages, William R. Jacobs Jr., Raul G. Barletta, Rupa Udani, John Chan, Gary Kalkut, Gabriel Sosne, Tobias Kieser, Gary J. Sarkis, Graham F. Hatfull, Barry R. Bloom

School of Veterinary and Biomedical Sciences: Faculty Publications

Effective chemotherapy of tuberculosis requires rapid assessment of drug sensitivity because of the emergence of multidrug-resistant Mycobacterium tuberculosis. Drug susceptibility was assessed by a simple method based on the efficient production of photons by viable mycobacteria infected with specific reporter phages expressing the firefly luciferase gene. Light production was dependent on phage infection, expression of the luciferase gene, and the level of cellular adenosine triphosphate. Signals could be detected within minutes after infection of virulent M. tuberculosis with reporter phages. Culture of conventional strains with antituberculosis drugs, including isoniazid or rifampicin, resulted in extinction of light production. In contrast, …

Canadian Cooperative Wildlife Health Centre Newsletter, Volume 2-2, May 1993

Canadian Cooperative Wildlife Health Centre: Newsletters and Publications

Work Underway at CCWHC Wildlife Pathologists at CCWHC An Outbreak of Duck Plague in New York State Snowy Owl Outbreak on the PrairiesCluster of bat rabies in red foxes in Prince Edward Island Adenoviral encephalitis in a red fox Carbofuran poisoning in herring gulls in Nova Scotia Protostrongylid infection in a Newfoundland moose Lead Poisoning in two Blue Jays Adrenal lesions in St. Lawrence Estuary beluga whale Cancer in a Red-Tailed Hawk and a Northern Goshawk Winter Mortality in Birds in Ontario Lead Poisoning in Waterfowl Sarcoptic Mange Crop necrosis and impaction in lesser Canada geese Black-tailed prairie dog mortality …

Wpa News 40 (1993), World Pheasant Association

Galliformes Specialist Group and Affiliated Societies: Newsletters

WPA News (May 1993), number 40

Published by the World Pheasant Association

The Phylogenetic Component Of Cooperative Breeding In Perching Birds: A Commentary, Deborah A. Mclennan, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The study of behavioral and ecological evolution within a phylogenetic context (historical ecology; Brooks and McLennan 1991) is an important component of comparative studies in evolutionary biology. Although the number of historical ecological studies is growing rapidly, this research field is still in its infancy--an infancy whose maturation is hampered by the absence of rigorous phylogenetic hypotheses for many of the groups that have traditionally fascinated behavioral ecologists. In the absence of such critical information, behavioral ecologists are faced with the options either of forming cooperative groups with phylogenetic systematists or of investigating their ecological data based on "trees" reconstructed …

Wildlife Health Centre Newsletter, Volume 1-2, Spring 1993

Canadian Cooperative Wildlife Health Centre: Newsletters and Publications

Wildlife Disease Investigation Manual
Directory of Wildlife Health Expertise
Our Information Specialist
Newfoundland and Labrador
Nova Scotia/New Brunswick
PEI
Pathology and Toxicology of Beluga Whales from the St. Lawrence Estuary, Quebec, Canada. Past, Present and Future.
Heartworm in Quebec Wildlife
Lyme borreliosis (Lyme Disease)
Salmonellosis in Pine Siskins in B.C.
Polioencephalomalacia (PEM) in White-tailed deer and Pronghorns
Canine Parvovirus (CPV) Enteritis in Two Coyotes
News from the National Wildlife Health Research Center, U.S. Fish & Wildlife Service, Madison, Wisconsin. Contributed by T. Roffe.

Coccidia (Apicomplexa), Genetic Diversity, And Environmental Unpredictability Of Four Chromosomal Species Of The Subterranean Superspecies Spalax Ehrenbergi (Mole-Rat) In Israel, Lee Couch, Donald W. Duszynski, Eviatar Nevo

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Forty-five mole-rats, representing 4 chromosomal species (2n = 52, 54, 58, 60) of the superspecies Spalax ehrenbergi. were collected from 12 localities in Israel in 4 distinct climatic regions. Feces were examined for coccidian oocysts and 41 (91 %) were infected; 26 (63%) had multiple infections of up to 5 coccidian species, 4 of which are described here as new species. Sporulated oocysts of Eimeria anzanensis n. sp. were ellipsoidal 18.3 x 12.5 μm (14-22 x 10-16) and had elongate-ovoidal sporocysts 7.3 x 4.9 μm (5-10 x 3-7); it occurred in 39 of 45 (87%) mole-rats, including all chromosomal …

Management Of University Biological Collections: A Framework For Policy And Practice, Patrick E. Mcguire, Lynn S. Kimsey, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A broad-based task force, chaired by Lynn Kimsey of the Department of Entomology and director of the Bohart Entomological Museum, was convened to review and assess the status of living and preserved biological collections held on the Davis campus of the University of California. The task force addressed three basic issues, forming subcommittees to 1) review the data gathered in a survey by the Genetic Resources Conservation Program of living and preserved collections and report its findings, 2) make recommendations for policy guidelines on a campus basis and an individual-collection basis, and s) propose a campus-wide administrative structure to address …

G93-1170 Bacteria In Milk Sources And Control, Gerald R. Bodman, Duane N. Rice

University of Nebraska-Lincoln Extension: Historical Materials

Bacterial contamination reduces the quality of milk and can cause serious health problems. This NebGuide discusses common causes of contamination as well as prevention.

Bacteria are single-cell organisms that multiply by dividing to form two cells (a process known as mitosis). Under optimum conditions, many bacteria can double in number each 20 minutes. That means one bacterium can result in up to 16,000,000 bacteria in just eight hours. They are microscopic in size, i.e., an individual bacterium cannot be seen without the aid of a microscope. They are so small that a single drop of water from a cow's udder …

Genetic Identificationo F A Hantavirusa Ssociated With An Outbreako F Acute Respiratoryi Llness, Stuart T. Nichol, Christina F. Spiropoulou, Sergey Morzunov, Pierre E. Rollin, Thomas G. Ksiazek, Heinz Feldmann, Anthony Sanchez, James E. Childs, Sherif Zaki, Clarence J. Peters

Zoonotics and Wildlife Disease: Publications

A mysterious respiratory illness with high mortality was recently reported in the southwestern United States. Serologic studies implicated the hantaviruses, rodent-borne RNA viruses usually associated elsewhere in the world with hemorrhagic fever with renal syndrome. A genetic detection assay amplified hantavirus-specific DNA fragments from RNA extracted from the tissues of patients and deer mice (Peromyscus maniculatus) caught at or near patient residences. Nucleotide sequence analysis revealed the associated virus to be a new hantavirus and provided a direct genetic link between infection in patients and rodents.

G93-1148 Common Infectious Diseases That Cause Abortions In Cattle, Duane N. Rice, Douglas Rogers

University of Nebraska-Lincoln Extension: Historical Materials

This NebGuide briefly discusses the common infectious diseases that cause abortions in cattle. "Abortion" is the expulsion of the fetus prior to the end of the normal gestation period. Many abortions occur very early after conception and the embryos or fetuses are so small that they may not be seen. Other abortions may occur near normal calving time and it is difficult to determine whether the cow has aborted or whether a premature birth has occurred. Abortions have many causes which may include physiological problems (such as hormonal imbalances), metabolic problems, toxicoses and/or infectious diseases caused by protozoa, bacteria or …

Infection With A Ratborne Hantavirus In Us Residents Is Consistently Associated With Hypertensive Renal Disease, Gregory E. Glass, Alan J. Watson, James W. Leduc, Gabor D. Kelen, Thomas C. Quinn, James E. Childs

Zoonotics and Wildlife Disease: Publications

A survey of 8080 subjects was conducted in Baltimore, examining the association between infection with hantaviruses and renal disease. Two groups (N = 6060) with no known risk factors were selected to establish a baseline antibody prevalence. Overall, antibody prevalence was 0.25%. Seroprevalence increased with age, without sex- or race-related differences. Patients with proteinuria showed the same patterns of infection but were more commonly seropositive (1.46%) than the reference group( OR, 3.23; P < .05). Infection among dialysis patients with end-stage renal disease was 2.76%, significantly higher than in the reference group( OR, 5.03; P < .05). In the proteinuria and the dialysis groups, hantavirus infection was consistently associated with a diagnosis of hypertensive renal disease. The association was unrelated to other chronic renal disease diagnoses. Overall, 6.5% of patients with end-stage renal disease due to hypertension were seropositive for a hantavirus. These data suggest that hantavirus infection is associated with hypertensive renal disease.

Exploiting Structural Differences Among Heteroduplex Molecules To Simplify Genotyping The Dqa1 And Dqb1 Alleles In Human Lymphocyte Typing, Peter A. Zimmerman, Mary Carrington, Thomas B. Nutman

United States Public Health Resources

A novel approach to DNA probe hybridization and heteroduplex analysis, termed directed heteroduplex analysis (DHDA) is presented here to illustrate its utility in simplification of human lymphocyte antigen (HLA)- typing. By strategic labeling of single-stranded probe sequences, DHDA allows the identification of specific heteroduplex structures that contribute to the differentiation of DQAI and DQB1 alleles. Because of the high degree of polymorphism among major histocompatibility complex class 11 second exon sequences, this analysis of 50 different heteroduplex molecules provides evidence of the importance of unpaired bases and mismatched base pairs and their effect on heteroduplex electrophoretic-mobility differences. This strategy is …

Book Review: The Baculovirus Expression System: A Laboratory Guide (1992) King, L. A. & Possee, R. D., David D. Dunigan

Nebraska Center for Virology: Faculty Publications

The power of molecular biology is unleashed with the ability to clone and sequence genes, and then express these genes in heterologous systems. This sets the stage for the full analysis of proteins that are otherwise difficult to isolate and/or purify, especially when present at very low copy number per cell or when isolated from relatively precious materials. Overexpression of protein is now possible in a number of systems including prokaryotes (e.g., E. coli) and various eukaryotes (yeast, insects, and plants). The issue then becomes, which system (1) most closely reflects the homologous expression with respect to posttranslational modifications, …

Detection Of Serum Antibody Responses In Cattle With Natural Or Experimental Neospora Infections, Patricia Conrad, Karen Sverlow, Mark Anderson, Joan Rowe, Robert Bondurant, Gwen Tuter, Richard Breitmeyer, California Department Of Food And Agriculture, Animal Health Branch, Mark Thurmond, Alex Ardans, J. P. Dubey, Gerarld Duhamel, Bradd Barr

School of Veterinary and Biomedical Sciences: Faculty Publications

Parasite-specific antibody responses were detected using an indirect fluorescent antibody (IFA) test in cattle that were naturally or experimentally infected with Neospora parasites. The test was developed using Neospora tachyzoites isolated from an aborted bovine fetus and grown in bovine cell cultures (isolate BPA1). In all cases, infections were confirmed by the identification of Neospora tachyzoites and/or bradyzoite cysts in fetal or calf tissues using an immunoperoxidase test procedure. Fifty-five naturally infected cows that aborted Neospora-infected fetuses had titers of 320-5,120 at the time of abortion. The titer of 6 cows that were serologically monitored over a prolonged period decreased …

Spiculopteragia Spiculoptera And S. Asymmetrica (Nematoda: Trichostrongyloidea) From Red Deer (Cervus Elaphus) In Texas, Lora G. Rickard, Eric P. Hoberg, Nancy M. Allen, Gary L. Zimmerman, Thomas M. Craig

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Specimens of Spiculopteragia spiculoptera and S. asymmetrica were recovered from the abomasa of five of ten naturally infected red deer (Cervus elaphus) in Texas (USA). Female specimens of Spiculopteragia were present in all five animals. Male specimens of S. spiculoptera and S. asymmetrica were present in one of five and three of five red deer, respectively. Spiculopteragia spiculoptera has not previously been recognized in the United States and the present report constitutes the first records of Spiculopteragia spp. in red deer from North America. It is likely that species of Spiculopteragia have been introduced to North America with …

Enteric Coccidia (Apicomplexa) In The Small Intestine Of The Northern Spotted Owl (Strix Occidentalis Caurina), Eric P. Hoberg, R. J. Cawthorn, O. R. Hedstrom

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Sporulated oocysts (mean dimensions = 13.0 x 10.8 μm) and sporocysts (11.3 x 5.5 μm) of a coccidian resembling Frenkelia sp. or Sarcocystis sp. were present in the lamina propria of the small intestine of a naturally-infected northern spotted owl (Strix occidentalis caurina) collected near Medford, Oregon, USA. Dimensions of these oocysts and sporocysts appear to be considerably smaller than those from other sarcocystid species with avian definitive hosts. Additionally, numerous developmental stages and unsporulated oocysts (mean dimensions 22.8 x 17.8 μm) of a possible species of Isospora also were observed in the intestinal epithelium. This constitutes the …

Book Review Of Parascript: Parasites And The Language Of Evolution By D. R. Brooks And D. A. Mclennan, Smithsonian Institution Press, Washington, D.C., 1993., Eric P. Hoberg

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Review of Parascript: Parasites and the Language of Evolution by Daniel R. Brooks and Deborah A. McLennan, with bibliographical references.

Molecular Approaches For Control Of Tomato Bushy Stunt Virus, H. B. Scholthof, M. Borja, Thomas Jack Morris, A. O. Jackson

School of Biological Sciences: Faculty Publications

I N this overview strategies are discussed that are currently being tested in our laboratory in an effort to engineer resistance against tomato bushy stunt virus (TBSV) in plants. TBSV is chosen as a model-system since this is an economically important RNA virus that is well characterized at the molecular level and the virus has some interesting and potentially useful features that can be exploited to produce resistant plants. Moreover, we anticipate that the information derived from our studies with TBSV will be applicable in developing resistance to other plant viruses. Some approaches that we are attempting to apply to …

Charles Eric "Chuck" Dawson, 1922-1993 [In Memoriam], Robin M. Overstreet, Stuart G. Poss

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Obituary of Charles Eric "Chuck" Dawson, 1922-1993, American marine ichthyologist.

Three New Species Of Nematodes Associated With Endemic Grape (Vitis) In California, Luma Al-Banna, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Three new species of nematodes were encountered during a study of natural diversity of nematode associates of native species of Vitis L. in California. Achromadora walkeri sp. n. was found in rhizosoil of the native California grape Vitis californica Bentham and is characterized by the position of the amphid (within the vicinity of both dorsal and ventral teeth), a relatively long stoma, and the absence of a prerectum. The other 2 species were plant parasitic criconematids: Criconemoides featherensis sp. n., found in association with roots of V. californica, is characterized by possessing strongly retrose annuli posterior to the vulva, …

The Probe, Issue 128 - December 1992

The Probe: Newsletter of the National Animal Damage Control Association

Contents:

Reflections On An Onerous Deed
CALENDAR OF UPCOMING EVENTS
Coyote Urine Translocated by Flora
PETA Indictments Expected Soon
Plague Claims Bobcat Hunter
Colorado Voters Ban Spring Hunt; Arizonans Defeat Trapping Ban
Charter Member Says Good-Bye
Probe Should Be Advocate for ADC Activities
Bill Fitzwater III
Next Issue Jan/Feb
NADCA Renewals and Donations
New Logo Chosen for NADCA!

Loss Of Infectivity By Progeny Virus From Alpha Interferon- Treated Human Immunodeficiency Virus Type 1-Infected T Cells Is Associated With Defective Assembly Of Envelope Gp120, Brian D. Hanson, Peter L. Nara, Radha K. Maheshwari, Girmel S. Sidhu, John G. Bernbaum, David Hoekzema, Monte S. Meltzer, Howard Gendelman

Nebraska Center for Virology: Faculty Publications

Levels of human immunodeficiency virus (HIV) DNA, RNA, or p24 antigen and reverse transcriptase activity in T-cell cultures treated with 500 IU of recombinant alpha interferon (rIFNα) per ml were comparable to those in control cultures. Radioimmunoprecipitation analysis of proteins in lysates of IFN-treated T cells documented a marked accumulation of HlV proteins. Localization of gp120 by immunofluorescence showed a diffuse pattern in IFN-treated cells quite distinct from the ring pattern in untreated control cells. That large quantities of a120 in aberrant cell compartments might affect HlV morphogenesis was confirmed in infectivity studies: virions from IFN-treated cells were 100- to …

Dynamics Of The Arctic Fox Population On St. Lawrence Island, Bering Sea, Francis H. Fay, Robert L. Rausch

Harold W. Manter Laboratory of Parasitology: Faculty Publications

English abstract: We hypothesized that the arctic fox, Alopex lagopus (Linnaeus), population on St. Lawrence Island was cyclic and that its fluctuations in size. structure, and productivity were correlated with the relative size of the population of northern voles, Microtus oeconomus Pallas, the primary prey. Based on a nine-year study, we determined that the variations in size of the fox and vole populations were similar, but they both were of low amplitude and not closely correlated. The high pregnancy rate (mean, 86%/yr) and numbers of young conceived (mean, 11.5/pregnancy) did not vary significantly among years, probably because of the consistently …

Profile: Gerald D. Schmidt (1934-1990), Scott Lyell Gardner, John E. Ubelaker

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Gerald D. Schmidt was born March 12, 1934 in Greeley, Colorado. After finishing his undergraduate degree in Biology at Colorado College in Greeley (now the University of Northern Colorado) Dr. Schmidt went on to receive his masters degree from Colorado State University (CSU) in 1962 and his PhD in 1964 also from CSU. While At CSU he worked under Dr. O. W. Olsen, first on the helminth parasites of the common snipe, and then, for his doctorate, he studied the embryological development of the Acanthocephala. He became a member of the faculty of UNC in Greeley In 1966. His collaboration …

Dynamics Of The Parasite Assemblage Of Pimephales Promelas In Nebraska, Mary Ann Mcdowell, Michael T. Ferdig, John J. Janovy Jr.

John J. Janovy, Jr. Publications

Present theory states that in species-poor assemblages interactions among species are negligible and assemblage structure is a result of host/parasite interactions. A corollary to this theory is that parasite species assemblages should behave as accumulations of multiple-kind items drawn from a population in which only the relative probability of drawing each kind varies. This study was designed to test this prediction by comparing descriptor values, including diversity indices, species density values, equitability, and prevalence to those obtained from data produced by a Monte Carlo simulation model. The parasite assemblage consisted of 7 parasite species or types found in the fathead …

Larids, Scolopacids, And Passerines Exploiting Ephemeral Prey At Talan Island, Russia, Eric P. Hoberg, Alexander I. Kondratiev, Alexander S. Kitaysky

Harold W. Manter Laboratory of Parasitology: Faculty Publications

We report an unusual multispecies foraging assemblage that included larids, shorebirds and passerines which exploited a highly concentrated and ephemeral prey source over a period of three days in July and August 1988. During studies of breeding biology, food-habits and host-parasite ecology among a diverse colonial avifauna at Talan Island in the northern Sea of Okhotsk (59°18'N; 149°02'E) we observed the formation of mixed-species flocks during extreme high tides of July 31, August 1, and August 13, 1988.

The U.S. National Parasite Collection--A Century Of Service, J. Ralph Lichtenfels, P. A. Pilitt, Eric P. Hoberg

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The U.S. National Parasite Collection will complete its first century of service to the field of animal parasitology in 1992. A brief history of the collection and a description of current policies on deposit and loan of specimens are provided. The collection, started in 1892 by Charles Wardell Stiles and Albert Hassall, now includes several constituent collections: The USNM Helminthological Collection, The USDA Parasite Collection, The Hoffman-Bangham Collection of Parasites of Freshwater Fish, and The Southeastern Cooperative Wildlife Diseases Study Collection of Parasites of White-tailed Deer. Major personal collections have been donated by F. W. Douvres, J. H. Fischthal, A. …

A New Species Of Linstowia (Cestoda: Anoplocephalidae) From Marsupials In Bolivia, Scott Lyell Gardner, Mariel L. Campbell

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A new species of cestode of the genus Linstowia (Cestoda: Anoplocephalidae) is described from marsupials of the genera Thylamys and Monodelphis. The new species (Linstowia schmidti) differs from L. iheringi Zschokke, 1904, in having a much smaller strobila and reduced number of proglottids, and in the distribution of the eggs in gravid proglottids. In Bolivia, cestodes of the genus Linstowia appear to have a restricted geographic distribution, occurring in marsupials only in southeastern Bolivia near the western margin of the Chaco. This host-parasite association may represent an ecological-historical relict.

Critical Comment: Designation And Curatorial Management Of Type Host Specimens (Symbiotypes) For New Parasite Species, Jennifer K. Frey, Terry L. Yates, Donald Duszynski, William L. Gannon, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The accurate identification of a host organism is an important component in the taxonomic recognition of a new species of parasite. Correct identification, curatorial management, and safekeeping of the host specimen from which a parasite type specimen is collected is also desirable. We recommend that the host from which the type of a new parasite species is described should be designated as a symbiotype.