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Endeavors 2003-04

Endeavors: Highlights of University of Nebraska-Lincoln Agricultural Research Division Research

CONTENTS:

ARD shares discoveries with citizens statewide
Building herbicide resistance into broadleaf crops
Carbon sequestration research points to storage potential
Identifying important genes could lead to controls for dangerous Listeria bacteria
Better understanding selenium’s benefits
A clearer look at root pests’ noshing habits
Vitamin may have role in preventing cancer
New tool helps check buffer strips’ effectiveness
Pinpointing genetic key to sterile plants
Major study yields info to enhance child care
Findings help protect allergic consumers
Insecticide resistance offers clues to controls
Making the most of bromegrass pastures
WeedSOFT takes guesswork out of weed control
New E. coli controls showing …

American Society Of Parasitologists Newsletter, V. 26, No. 4, Summer 2004, Scott Lyell Gardner

American Society of Parasitologists: Newsletter

An issue of the American Society of Parasitologists' quarterly newsletter, also called the Journal of Parasitology Newsletter.

Office Of Research And Graduate Studies -- Annual Report 2003-2004

Office of Research and Economic Development: Publications

Contents
Science and Engineering page
Recombinant Bandage 02
Advanced Computing, Everyday Life 04
Software Engineering 05
Silicon Nanotubes. 06
Nutritional Genomics and Nanomaterials. 08
Genetics of Virulence 09
Rapid PCR Device. 10
Selenium, Cancer and Aging 12
Education
Math in the Middle Institute. 14
Project Fulcrum 16
School Readiness for Parents 17
Behavioral Science
Assessing Threatening Behavior 18
Family Dynamics of Infertility 19
Arts & Humanities
Encyclopedia of the Great Plains 20
Global Politics 22
Sculpture Conservation. 22
Commission with Philip Glass 23
Rising Stars page
Luminescence. 24
Giant Thunderstorms 24
Debugging Software 25
Technology Development
Robotic Traffic Barrels 26 …

Department Of Veterinary And Biomedical Sciences 2004 Annual Report

School of Veterinary and Biomedical Sciences: Information and History

Veterinary and Biomedical Sciences Personnel
VBMS Teaching Program
VBMS Research Program
International Activities, 2004
Veterinary Extension Program. 2004
VBMS Grants and Contracts Program. 2004
Refereed Publications by VBMS Faculty in 2004
Department of Veterinary and Biomedical Sciences. Selected Committees, Editorial and Other Appointments. 2004
Articles Regarding the Department in 2004
Departmental Budget Summaries. 2004
Nebraska Agricultural Statistics. 2003/2004

Primaquine Therapy For Malaria, J. Kevin Baird, Stephen L. Hoffman

United States Public Health Resources

Primaquine is the only available drug for preventing relapse of malaria, and confusion surrounds its use. This review examines the wide range of clinical applications of primaquine described in the medical literature between 1946 and 2004. The risk of relapse of Plasmodium vivax malaria without primaquine therapy ranged from 5% to 80% or more, depending largely upon geographic location. Supervision of therapy profoundly impacts the risk of relapse, and almost all reports of malaria resistant to primaquine are associated with lack of such supervision. We nonetheless suspect that there is widespread resistance to the standard course of primaquine therapy, which …

West Nile Virus Blood Transfusion-Related Infection Despite Nucleic Acid Testing, Alexandre Macedo De Oliveira, Brady D. Beecham, Susan P. Montgomery, Robert S. Lanciotti, Jeffrey M. Linnen, Cristina Giachetti, Larry A. Pietrelli, Susan L. Stramer, Thomas J. Safranek

United States Public Health Resources

BACKGROUND: A case of West Nile virus (WNV) encephalitis associated with transfusion of blood that did not react when tested for WNV by minipool (MP) nucleic acid testing (NAT) is described. A Nebraska man developed clinical encephalitis 13 days after surgery and transfusion of 26 blood components. Antibody testing confirmed WNV infection. An investigation was initiated to determine the source of this infection.

STUDY DESIGN AND METHODS: The patient’s family members were interviewed to identify risk factors for WNV infection. Residual samples were retested for WNV RNA using transcription-mediated amplification (TMA) assay and two polymerase chain reaction (PCR) assays. …

Scwds Briefs: Volume 19, Number 4 (January 2004)

Southeastern Cooperative Wildlife Disease Study: Publications

• A highly pathogenic avian influenza (HPAI) virus (H5N1) recently has been reported among domestic poultry in Cambodia, China, Indonesia, Japan, Laos, South Korea, Thailand, and Vietnam. This virus also has been responsible for 23 confirmed human cases, including 18 deaths.
• U.S. Secretary of Agriculture Ann Veneman announced a “presumptive positive” case of bovine spongiform encephalopathy (BSE) in a dairy cow from the state of Washington.—Report on completed investigation.
• Proposed Rule in the Federal Register regarding a chronic wasting disease (CWD) herd certification program and interstate shipping requirements for captive deer and elk.
• Another BIG Year for …

Animal Science Alumni Newsletter, Winter 2004

Department of Animal Science: Departmental News

Contents:

Four Animal Science Faculty Awarded Omtvedt lnnovation Awards
Animal Science Leadership Academy
From the Department Head’s Desk
New Faculty
Animal Science Leadership Academy
Alum Earns First Degree in Culinary
Middle School Science Teacher Learns about Research First Hand
Sahs Exhibition Hall Named After Former Director
Former Department Head to Retire From University of Wisconsin
Animal Science Graduate Student Association (ASGSA)
Bud Britton Golf Classic
ASGSA Activities
Ambassador Activities
Equestrian Team Successful Again
Sixth Annual Block and Bridle Steak Fry
Recent Department Awards and Recognitions
Nold Receives Distinguished Specialist Award
Wolverton 2004 4-H Hero's Award
Notable Undergraduate
Beef Top 40 …

Analysis Of Free Analyte Fractions By Rapid Affinity Chromatography, David S. Hage, William A. Clarke

Department of Chemistry: Faculty Publications

The invention is generally directed toward an analytical method to determine the concentration of the free analyte fraction in a sample. More particularly, the method encompasses applying a sample comprising a free and bound analyte fraction to an affinity column capable of selectively extracting the free fraction in the millisecond time domain. The signal generated by the free fraction is then quantified by standard analytical detection techniques. The concentration of the free fraction may then be determined by comparison of its signal with that of a calibration curve depicting the signal of known concentration of the same analyte.

Phosphorylation Of Vesicular Stomatitis Virus Phosphoprotein P Is Indispensable For Virus Growth†, Subash C. Das, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

The phosphoprotein (P) of vesicular stomatitis virus (VSV) is an essential subunit of the viral RNAdependent RNA polymerase (RdRp) complex. It is phosphorylated at two different domains. Using defective interfering (DI) RNA or minigenomic RNA templates, we previously demonstrated that phosphorylation within the amino-terminal domain I is essential for transcription, whereas phosphorylation within the carboxyterminal domain II is necessary for replication. For the present study, we examined the role of the phosphorylation of residues in these domains in the life cycle of VSV. Various mutant P coding sequences were inserted into a full-length cDNA clone of VSV, and the virus …

The European Court Of Human Rights: Chechnya's Last Chance?, Tarik Abdel-Monem

University of Nebraska Public Policy Center: Publications

Since 1994, the wars between the Russian military and Chechen nationalist forces have resulted in "butchery and savagery on a scale and intensity recalling World War II" An estimated 100,000 persons may have been killed so far, the vast majority being civilians? In relation to other international crises, the conflict in Chechnya has been largely muted in the press, and the international community's reaction has been to marginalize the conflict as an "internal matter" of Russian affairs. In January 2003, for the first time, the Strasbourg-based European Court of Human Rights (ECIIR or the Court) declared six petitions alleging the …

Bovine Tuberculosis (Mycobacterium Bovis) In Wildlife In Spain, Alicia Aranaz, Lucía De Juan, Natalia Montero, Celia Sanchez, Margarita Galka, Consuelo Delso, Julio Alvarez, Beatriz Romero, Javier Bezos, Ana I. Vela, Victor Briones, Ana Mateos, Lucas Domínguez

Michigan Bovine Tuberculosis Bibliography and Database

Mycobacterium bovis infection in wildlife and feral species is a potential source of infection for livestock and a threat to protected and endangered species. The aim of this study was to identify Spanish wild animal species infected with M. bovis through bacteriological culture and spacer oligonucleotide typing (spoligotyping) of isolates for epidemiological purposes. This study included samples from red deer (Cervus elaphus), fallow deer (Dama dama), wild boar (Sus scrofa), Iberian lynx (Lynx pardina), hare (Lepus europaeus), and cattle (Bos taurus). They were collected in several geographical areas …

Primaquine Therapy For Malaria, J. Kevin Baird, Stephen L. Hoffman

United States Navy: Publications

Primaquine is the only available drug for preventing relapse of malaria, and confusion surrounds its use. This review examines the wide range of clinical applications of primaquine described in the medical literature between 1946 and 2004. The risk of relapse of Plasmodium vivax malaria without primaquine therapy ranged from 5% to 80% or more, depending largely upon geographic location. Supervision of therapy profoundly impacts the risk of relapse, and almost all reports of malaria resistant to primaquine are associated with lack of such supervision. We nonetheless suspect that there is widespread resistance to the standard course of primaquine therapy, which …

Effect Of Increasing Dietary Crude Protein Concentration On Growth Performance And Serum Insulin-Like Growth Factor-I Concentration, Robert L. Fischer, Phillip S. Miller

Nebraska Swine Reports

This study was conducted to investigate the effects of increasing dietary protein intake on growth performance and serum insulin-like growth factor-I (IGF-I) concentration in growing-finishing gilts. Thirty-nine crossbred gilts with an initial body weight of 74.3 lb were used in a 28-day growth study. The gilts were randomly allocated to one of five dietary treatments. The diets were standard corn soybean meal diets, which were formulated to contain 10, 14, 18, 22, or 26% crude protein by changing the ratio of corn to soybean meal in the diet. Pig and feeder weights were recorded weekly for the determination of average …

Histamine As An Aid To Biopsy Of Third Eyelid Lymphoid Tissue In Sheep, S. Bender, J. Alverson, L. M. Herrmann, Katherine I. O'Rourke

Zoonotics and Wildlife Disease: Publications

TRANSMISSIBLE spongiform encephalopathies, of which ovine scrapie is a prototype, are neurodegenerative disorders characterised by the accumulation of an abnormal isoform (PrP-scrapie or PrP^Sc) of a normal cellular protein (PrP^C) (Prusiner 1982). Characteristics of the disease include long incubation periods, and a progressive and chronic clinical course resulting in death (Fraser 1976). PrP^sc is detectable during the preclinical stages of scrapie in peripheral lymph nodes (Ikegami and others 1991, Muramatsu and others 1993), in lymphoid follicles of the tonsils (Schreuder and others 1996) and in lymphoid follicles of the nictitating membrane or third eyelid (O'Rourke …

Guidelines For Managing Lesser Prairie-Chicken Populations And Their Habitats, Christian A. Hagen, Brent E. Jamison, Kenneth M. Giesen, Terry Z. Riley

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Lesser prairie-chicken (Tympanuchus paidicinctus) populations have declined by >90% since the 1800s. These declines have concerned both biologists and private conservation groups and led to a petition to list the lesser prairie-chicken as threatened under the Endangered Species Act. Most of the land in the current range of the lesser prairie-chicken is privately owned, and declines have been primarily attributed to anthropogenic factors. Conversion of native rangeland to cropland and excessive grazing have been implicated as leading causes in the species' decline. Periodic drought probably has exacerbated these problems. Little research on habitat requirements was conducted prior to …

The Southwestern Association Of Parasitologists: The First 35 Years: 1967–2002, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

First two paragraphs:

It is likely that the idea to organize southwestern parasitologists into a regional group originated over cocktails one night in the late 1950s during a regional or national meeting. What is clear is that two men—Drs. Robert E. Kuntz and Donald V. Moore— were instrumental in turning the idea into what has become, in reality, the Southwestern Association of Parasitologists (SWAP). Dr. J. Teague Self (personal communication, 1981) stated, “The beginning of SWAP was an idea of Robert Kuntz who felt that something could be gained if several of us here in the southwest could get together …

Defeating Diplostomoid Dangers In Usa Catfish Aquaculture, Robin M. Overstreet, Stephen S. Curran

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Diplostomoid digenean metacercariae have caused widescale mortalities of channel catfish, Ictalurus punctatus (Rafinesque), at aquaculture farms in Louisiana, Mississippi and Arkansas, USA. Originally, based on a tentative diagnosis, the industry considered the primary harmful agent to be an introduced species from Europe, Bolbophorus confusus (Krause, 1914), frequently reported from the American white pelican, Pelecanus erythrorhynchos Gmelin. Our group has now shown, using ITS 1-2 plus three more-conservative gene fragments, that two sympatric species of Bolbophorus exist in the American white pelican. One, B. damnificus Overstreet et Curran, 2002, infects the musculature of catfish, and the other, probably not B. confusus …

Paneak's Plants And Animals [In A Hungry Country: Appendix 1], Robert L. Rausch

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A list of plants and animals recognized by Simon Paneak (1900-1975), a Nunamiut Eskimo. Names are given in English and Iñupiaq, and their scientific names are provided.

A Survey Of Antibiotic Resistance Among Coliform Bacteria Isolated From The Missouri River, Sara E. Mcdonnell, Amy M. Treonis

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

The prevalence of antibiotic resistant microorganisms in the environment is not well known but could represent a challenge to maintaining public health in the future. Fecal waste from livestock facilities, where antibiotics are routinely used, is recognized as a significant source of pollution to surface waters in the United States. We collected water from the Missouri River across a winter to spring seasonal change in order to survey the density of coliform bacteria. We tested bacterial isolates for resistance to penicillin, tetracycline, ampicillin, erythromycin, and ciprofloxacin. Coliform density in Missouri River water generally was high throughout the study (0-405 cfu …

Cd46-Mediated Transduction Of A Species D Adenovirus Vaccine Improves Mucosal Vaccine Efficacy, Zenaido T. Camacho, Mallory A. Turner, Michael A. Barry, Eric A. Weaver

Nebraska Center for Virology: Faculty Publications

The high levels of preexisting immunity against Adenovirus type 5 (Ad5) have deemed Ad5 unusable for translation as a human vaccine vector. Low seroprevalent alternative viral vectors may be less impacted by preexisting immunity, but they may also have significantly different phenotypes from that of Ad5. In this study we compare species D Ads (26, 28, and 48) to the species C Ad5. In vitro transduction studies show striking differences between the species C and D viruses. Most notably, Ad26 transduced human dendritic cells much more effectively than Ad5. In vivo imaging studies showed strikingly different transgene expression profiles. The …

Phylogenetic Analyses Of Texas Isolates Indicate An Evolving Subtype Of The Clade B Feline Immunodeficiency Viruses, Eric A. Weaver, Ellen W. Collisson, Margaret Slater, Guan Zhu

Nebraska Center for Virology: Faculty Publications

Rigorous phylogenetic analyses were used to compare the nucleotide sequences of feline immunodeficiency virus strains isolated from Texas and throughout the world. The envelope V3-V4 sequences and capsid gene of the Texas isolates formed a cluster between subtypes B and E. Statistical comparisons with other published sequences confirmed that the Texas group is a unique cluster, possibly a new subtype, arising from subtype B.

The Southwestern Association Of Parasitologists: The First 35 Years: 1967–2002, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

First two paragraphs:

It is likely that the idea to organize southwestern parasitologists into a regional group originated over cocktails one night in the late 1950s during a regional or national meeting. What is clear is that two men—Drs. Robert E. Kuntz and Donald V. Moore— were instrumental in turning the idea into what has become, in reality, the Southwestern Association of Parasitologists (SWAP).

Dr. J. Teague Self (personal communication, 1981) stated, “The beginning of SWAP was an idea of Robert Kuntz who felt that something could be gained if several of us here in the southwest could get together …

Some Aspects Of The Biology And Life History Of Bothitrema Bothi (Monogenea: Bothitrematidae) From The Flounder Scophthalmus Aquosus (Bothidae) From New Jersey, Usa, Sherman S. Hendrix

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Bothitrema bothi (MacCallum, 1913), a monopisthocotylean monogenean (Bothitrematidae) found on gills and occasionally on the olfactory rosette within the nares of the bothid flounder, Scophthalmus aquosus (Mitchill, 1815), is distributed along the Atlantic coast of the USA from Woods Hole, Massachusetts to Delaware Bay. This study details aspects of the morphology, microecology and biology of the oncomiracidium, juvenile and adult. Adult worms occur almost exclusively between rakers on the first and second gill arches while juveniles occupy secondary gill filaments. Analysis of variance revealed significant arch, side and position effects. Worm attachment and position is maintained largely by suction generated …

Cardicola Forsteri (Digenea: Sanguinicolidae) From The Heart Of A Northern Bluefin Tuna, Thunnus Thynnus (Scombridae), In The Northwest Atlantic Ocean, Stephen A. Bullard, Robert J. Goldstein, Robert H. Goodwin Iii, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

We report a specimen of Cardicola forsteri Cribb, Daintith, and Munday, 2000 (Digenea: Sanguinicolidae) from the lumen of the heart of a northern bluefin tuna, Thunnus thynnus (Linnaeus, 1758) (Scombridae), that was 218 cm in total length (TL) and caught in the northwest Atlantic Ocean 12 km south of Cape Lookout, North Carolina. The hearts of 12 similarly sized northern bluefin tuna (127–262 cm TL) from George’s Bank, northwest Atlantic Ocean, were not infected. This is the first report of C. forsteri from a wild host and of a sanguinicolid from any scombrid in the northwest Atlantic Ocean. Bluefin tuna …

Understanding Threats To Scientific Openness, James B. Petro, David A. Relman

United States Department of Veterans Affairs: Publications

The scientific community is being confronted by public concerns that freely available scientific information may be exploited by terrorists. Differing points of view among scientists threaten to complicate discussion intended to address these concerns. Skepticism of the existence, breadth, and severity of the threat posed by would-be bioweaponeers is compounded by the failure to find clear evidence of biological weapons in Iraq. Also, some even question the extent to which open-source scientific material contributes to the threat.

Recent public discussions regarding the potential for open-source science to enable bioterrorist activities have occurred in a vacuum, without examples of "real-world" activity. …

Bacteriolytic Activity Of Selected Vertebrate Sera For Borrelia Burgdorferi Sensu Stricto And Borrelia Bissettii, Amy J. Ullmann, Robert S. Lane, Klaus Kurtenbach, Michael Miller, Martin E. Schriefer, Nordin Zeidner, Joseph Piesman

United States Public Health Resources

An in vitro assay to evaluate the bacteriolytic activity of the complement pathway was applied to 2 strains of Borrelia bissettii, CO501 and DN127, and compared with that of B. burgdorferi sensu stricto B31. Sera from mule deer ( Odocoileus hemionus) and the Western Fence lizard ( Sceloporus occidentalis) were completely borreliacidal for B. burgdorferi and for both strains of B. bissettii. Serum from Bobwhite quail ( Colinus virginianus) was nonlytic for B. burgdorferi and partially lytic for B. bissettii strains, CO-501 and DN127. Serum from a New Zealand White rabbit ( Oryctolagus cuniculus) …

Acceptance Of The Clark P. Read Mentor Award: The Teague Self Lessons, John J. Janovy Jr.

John J. Janovy, Jr. Publications

The transcript of John J. Janovy Jr.'s speech upon acceptance of the American Society of Parasitologists' Clark P. Read Mentor Award, 2003.

A New Species Of Mathevotaenia (Cestoda: Anoplocephalidae) And Other Tapeworms From Marsupials In Argentina, Scott Lyell Gardner, Mariel L. Campbell, Graciela T. Navone

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Cestodes are reported from Didelphis albiventris Lund, 1840 and Micoureus cinereus Temminck, 1824 (Marsupialia: Didelphidae) in Argentina. These include a new species of Mathevotaenia Akhumyan, 1946 (Cestoda: Anoplocephalata) as well as M. bivittata (Janicki, 1904) and an unknown hymenolepidid cestode. Mathevotaenia argentinensis n. sp. is characterized by a relatively narrow strobila, 18–37 mm in total length and 1.0–1.5 mm in maximum width, 135–163 craspedote proglottids, 19–27 testes, and a muscular genital atrium. This species differs from M. didelphidis (Rudolphi, 1819) in the disposition of the genital ducts between the excretory canals and in the entrance of the vagina into the …

Molecular Phylogeny Of Haematoloechus Looss, 1899 (Digenea: Plagiorchiidae), With Emphasis On North American Species, Daniel R. Brooks, Virginia León-Règagnon

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Phylogenetic hypothesis of 23 populations corresponding to 18 species of the digenean Haematoloechus from America, Europe, and Africa, based on ribosomal DNA 28S partial sequences (~890 bp), is presented. Genetic divergence between the in-group and the out-groups ranged from 9.7 to 14.5% and within the in-group, from 0.9 to 12.2%. Eight most parsimonious trees 569 steps long were obtained, with a consistency index of 72%. Groups in the tree are not congruent with those in previous classification schemes of species in the genus, based on a small number of morphological characters. For this subset of Haematoloechus,/i> species, plesiomorphic hosts are …