Digital Commons Network^™

Less Activity Means Improved Welfare? How Pair Housing Influences Pinyon Jay (Gymnorhinus Cyanocephalus) Behaviour, London M. Wolff, Jeffrey R. Stevens

Center for Brain, Biology, and Behavior: Faculty Publications

The activity level and specific behaviours exhibited by captive animals are crucial indicators of welfare. Stereotypies, or repetitive behaviours that have no apparent function or goal, are performed by animals experiencing poor conditions in their environment and indicate welfare concerns. Changes in the housing environment in particular may have critical influences on behaviour and welfare. Here, we measured behavioural changes in a captive pinyon jay (Gymnorhinus cyanocephalus) population (n = 10) associated with a shift from single to pair housing. Using automated video processing, we show that pair housing greatly reduced overall activity levels in these birds. The …

Subconjunctival Antimicrobial Poloxamer Gel For Treatment Of Corneal Ulceration In Stranded California Sea Lions (Zalophus Californianus), Claire A. Simeone, Carmen M. H. Colitz, Kathleen M. Colegrove, Cara L. Field, Carlos Rios, Heather L. Chandler, Shawn P. Johnson

United States Department of Commerce: Staff Publications

Objective Corneal ulcers are commonly encountered in pinnipeds. Prolonged oral antibiotics and topical ophthalmic solutions may not be practical to administer, and novel treatment techniques are desired. Thermodynamic gels are a potential solution because they hold antimicrobials at the site of injection, slowly releasing drug. This study investigated the clinical efficacy of antibiotic-impregnated poloxamer gel in management of corneal ulceration.

Animal studied Twenty-six California sea lions undergoing rehabilitation at The Marine Mammal Center.

Procedures A poloxamer gel mixed with 2% enrofloxacin was subconjunctivally injected in the treatment group. Control animals received oral doxycycline. Systemic antiinflammatories and analgesics were administered as …

Nebline, June/July 2024

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Per- and Polyfluoroalkyl Substances (PFAS) Facts Related to Drinking Water by Becky Schuerman

Tar Spot is an Emerging Corn Disease in Eastern Nebraska by John Nelson

Nutrition Education for Refugee and Immigrant Families in Lincoln by Emily Gratopp

Recipe of the Month: Biryani by Emily Gratopp

Garden Guide: Things to Do This Month

Sudden Wilt and Death in Tomatoes by Sarah Browning

Sun's Out, Fun's Out: Keeping Your Little One Safe This Summer by Hayley Perez

Protect Yourself from Bed Bugs by Kait Chapman

Heart of 4-H Volunteer Award: Netsy Barent and Jessica Marsh

Nebraska 4-H Foundation Scholarship Winners

Learn …

Cost Modeling Of Provision C.3 Green Stormwater Infrastructure Requirements For Residential Development In Concord, California, James H. Mizutani

Community and Regional Planning Program: Professional Projects

To assist the city of Concord in achieving its housing goals as presented in its general plan, while still meeting the stormwater discharge requirements laid out in the city’s municipal discharge permit, I worked with local developers and consultants to create a spreadsheet modeling tool that allows housing developers to estimate the total cost of green stormwater infrastructure needed to comply with local regulations.

This report contains a summary of the conditions leading to the necessity of this tool, the steps the tool takes that go beyond existing models, a description of how the model operates, instructions on how to …

Cost Modeling Of Provision C.3 Green Stormwater Infrastructure Requirements For Residential Development In Concord, California, James Henry Mizutani

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

To assist the city of Concord in achieving its housing goals as presented in its general plan, while still meeting the stormwater discharge requirements laid out in the city’s municipal discharge permit, I worked with local developers and consultants to create a spreadsheet modeling tool that allows housing developers to estimate the total cost of green stormwater infrastructure needed to comply with local regulations.

This report contains a summary of the conditions leading to the necessity of this tool, the steps the tool takes that go beyond existing models, a description of how the model operates, instructions on how to …

Neuropsychological Intra-Individual Variability In Traumatic Brain Injury: Clinical Utility, Classification, And Associations With Outcomes, Jeremy A. Feiger

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Traumatic brain injury (TBI) survivors experience a range of sequelae including neurobiological brain changes, cognitive impairment, psychological difficulties, and functional changes. Neuropsychological assessment after injury is used to characterize current strengths and weaknesses to inform prognosis and interventions. There is currently no reliable and consistent method of assessing and classifying injuries in the post-acute and chronic phases of moderate to severe TBI (msTBI) that is associated with long term psychosocial outcomes. This study aimed to combine the use of within-person variability on neuropsychological test batteries and in cognitive response latency, known as intra-individual variability (IIV), with a novel cognitive phenotyping …

A Mixed Method Examination Of Bipositivity As A Factor Protecting Cis, Trans, And Gender Expansive Plurisexual Women Against Harmful Sexual Violence Outcomes, Allison E. Cipriano

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Plurisexual women are more likely to experience sexual violence, post-traumatic stress (PTS), and alcohol use problems compared to women with single gender attraction. Disproportionately high rates of sexual violence may partially explain the prevalence of PTS and alcohol problems experienced by plurisexual women. Yet, research on sexual violence, PTS, and alcohol use problems have rarely specifically investigated these phenomena among plurisexual women. Researchers have identified a positive association between binegative experiences and risk of sexual violence among plurisexual women, suggesting that sexuality-based experiences may be connected to sexual violence victimization for this population. While identifying factors predicting risk of sexual …

The Mental Health Experience Of Transgender And Gender Diverse Colombians: Que La Diversidad No Cueste La Vida, María Natalia Acosta Canchila

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Experiences of TGD people in various countries reflect difficulties accessing mental health services and significant mental health needs. In Colombia, experiences in broader healthcare systems suggest various barriers to care that include experiences of discrimination. However, specific experiences in mental health services have not been thoroughly examined. The current study aimed to understand the experiences, perceptions, and needs of TGD people in Colombia in mental health systems. Qualitative data was collected via 40 semi-structured interviews with TGD people in Colombia, data was analyzed via reflexive thematic analysis. Findings suggests great mental health needs related to needing support in the process …

Harassment, Stalking, And Violence On Campus: An Examination Of Persistent Cases, Madeline M. Eyer

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Stalking, harassment, dating violence, physical assault, and sexual assault continue to occur at elevated frequencies on college campuses. Most subjects cease engagement in these behaviors within a short period; however, a subset of individuals will engage in these intrusive behaviors over an extended length of time. While persistence has been examined in the stalking literature, only one study has examined risk factors of persistence in a college sample, and no studies have examined persistence for adverse behaviors outside of stalking (i.e., harassment, dating violence, physical assault, and sexual assault). Given the risk for physical and psychological harm toward targets as …

An Inaugural Forum On Epidemiological Modeling For Public Health Stakeholders In Arizona, Joseph R. Mihalijevic, Carmenlita Chief, Mehreen Malik, Kayode Oshinubi, Eck Doerry, Esma Gel, Crystal Hepp, Tim Lant, Sanjay Mehrotra, Samantha Sabo

Department of Supply Chain Management and Analytics: Faculty Publications

Epidemiological models—which help us understand and forecast the spread of infectious disease—can be valuable tools for public health. However, barriers exist that can make it difficult to employ epidemiological models routinely within the repertoire of public health planning. These barriers include technical challenges associated with constructing the models, challenges in obtaining appropriate data for model parameterization, and problems with clear communication of modeling outputs and uncertainty. To learn about the unique barriers and opportunities within the state of Arizona, we gathered a diverse set of 48 public health stakeholders for a day-and-a-half forum. Our research group was motivated specifically by …

Plas 439: Organic Farming And Food Systems Faculty-Led Inquiry Into Reflective Scholarly Teaching Benchmark Portfolio, Christian Stephenson

UNL Faculty Course Portfolios

Organic Farming and Food Systems is a senior and graduate level course for students in the Department of Agronomy and Horticulture. This course was previously offered but has been significantly modified as I have taken on responsibility for the course. Goals for the course include student comprehension of the methods of organic and regenerative farming and the impacts of those methods on economic, environmental, and social sustainability. An additional goal is to build student competency in the evaluation of primary, secondary, and tertiary information resources and critical thinking surrounding issues in food production. Assessment for the course was through diverse …

What Climate Change Means For Nebraska

United States Environmental Protection Agency: Publications

Nebraska’s climate is changing. In the past century, most of the state has warmed by at least one degree (F). The soil is becoming drier, and rainstorms are becoming more intense. In the coming decades, flooding is likely to increase, yet summers are likely to become increasingly hot and dry, which would reduce yields of some crops, require farmers to use more water, and amplify some risks to human health.

Our climate is changing because the earth is warming. People have increased the amount of carbon dioxide in the air by 40 percent since the late 1700s. Other heat-trapping greenhouse …

Mitigating Identity-Related Anxiety Through Humor And Immersive Storytelling With 360-Degree Video In Virtual Reality: A Study On Microaggressions’ Mental Health Effects, Changmin Yan, Alan Eno, Adam Wagler

College of Journalism and Mass Communications: Faculty Publications

Background: Microaggressions are subtle slights that can cause significant psychological distress among marginalized groups. Few studies have explored interventions that might mitigate these effects. Objective: This study aimed to investigate if and how humor-infused immersive storytelling via virtual reality (VR) could reduce identity-related psychological distress caused by microaggressions. Methods: Using a community-based participatory research approach, we developed a 7-min 360-degree VR film depicting scenarios of microaggressions across various identities. Fortysix college students participated in a controlled study where they were exposed to this immersive VR experience. We measured identity-related psychological anxiety, character identification, perceived humor, and perceived …

Adaptation To Tolerate High Doses Of Arabinoxylan Is Associated With Fecal Levels Of Bifidobacterium Longum, Edward C. Deehan, Zhengxiao Zhang, Nguyen K. Nguyen, Maria Elisa Perez-Muñoz, Janis Cole, Alessandra Riva, David Berry, Carla M. Prado, Jens Walter

Department of Food Science and Technology: Faculty Publications

Dietary fiber supplements are a strategy to close the ‘fiber gap’ and induce targeted modulations of the gut microbiota. However, higher doses of fiber supplements cause gastrointestinal (GI) symptoms that differ among individuals. What determines these inter-individual differences is insufficiently understood. Here we analyzed findings from a six-week randomized controlled trial that evaluated GI symptoms to corn bran arabinoxylan (AX; n = 15) relative to non-fermentable microcrystalline cellulose (MCC; n = 16) at efficacious supplement doses of 25 g/day (females) or 35 g/day (males) in adults with excess weight. Self-reported flatulence, bloating, and stomach aches were evaluated weekly. Bacterial taxa …

United States National Aeronautics And Space Administration (Nasa) Scientific Integrity Policy

Copyright, Fair Use, Scholarly Communication, etc.

US NASA Scientific Integrity Policy

Bagging Improves The Performance Of Deep Learning-Based Semantic Segmentation With Limited Labeled Images: A Case Study Of Crop Segmentation For High-Throughput Plant Phenotyping, Yinglun Zhan, Yuzhen Zhou, Geng Bai, Yufeng Ge

Department of Statistics: Faculty Publications

Advancements in imaging, computer vision, and automation have revolutionized various fields, including field-based high-throughput plant phenotyping (FHTPP). This integration allows for the rapid and accurate measurement of plant traits. Deep Convolutional Neural Networks (DCNNs) have emerged as a powerful tool in FHTPP, particularly in crop segmentation—identifying crops from the background—crucial for trait analysis. However, the effectiveness of DCNNs often hinges on the availability of large, labeled datasets, which poses a challenge due to the high cost of labeling. In this study, a deep learning with bagging approach is introduced to enhance crop segmentation using high-resolution RGB images, tested on the …

Identification Of Carbohydrate Gene Clusters Obtained From In Vitro Fermentations As Predictive Biomarkers Of Prebiotic Responses, Car Reen Kok, Devin J. Rose, Juan Cui, Lisa Whisenhunt, Robert Hutkins

Department of Food Science and Technology: Faculty Publications

Background Prebiotic fibers are non-digestible substrates that modulate the gut microbiome by promoting expansion of microbes having the genetic and physiological potential to utilize those molecules. Although several prebiotic substrates have been consistently shown to provide health benefits in human clinical trials, responder and non-responder phenotypes are often reported. These observations had led to interest in identifying, a priori, prebiotic responders and non-responders as a basis for personalized nutrition. In this study, we conducted in vitro fecal enrichments and applied shotgun metagenomics and machine learning tools to identify microbial gene signatures from adult subjects that could be used to predict …

Understanding The Experience Of Bullying Of Students With Disabilities: A Phenomenological Study, Miriam Patricia Brenda Crinion

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

The purpose of the dissertation study was to increase understanding of the social experiences of students with disabilities. Students with disabilities are at increased risk of being involved in the bullying dynamic and experiencing greater social difficulties relative to their typically developing peers. Consequently, students with disabilities are more likely to report adverse mental health outcomes due to bullying involvement. The current literature suggests the effectiveness of traditional bullying intervention programming for students with disabilities is limited. Students with disabilities may also perceive traditional coping strategies to be inadequate and may have their own unique coping strategies to help them …

Descspim: An Affordable And Easy-To-Build Light-Sheet Microscope Optimized For Tissue Clearing Techniques, Kohei Otomo, Takaki Omura, Yuki Nozawa, Steven J. Edwards, Yukihiko Sato, Yuri Saito, Shigehiro Yagishita, Hitoshi Uchida, Yuki Watakabe, Kiyotada Naitou, Rin Yanai, Naruhiko Sahara, Satoshi Takagi, Ryohei Katayama, Yusuke Iwata, Toshiro Shiokawa, Yoku Hayakawa, Kensuke Otsuka, Haruko Watanabe-Takano, Yuka Haneda, Shigetomo Fukuhara, Miku Fujiwara, Takenobu Nii, Chikara Meno, Naoki Takeshita, Kenta Yashiro, Juan Marcelo Rosales Rocabado, Masaru Kaku, Tatsuya Yamada, Yumiko Oishi, Hiroyuki Koike, Yinglan Cheng, Keisuke Sekine, Jun-Ichiro Koga, Kaori Sugiyama, Kenichi Kimura, Fuyuki Karube, Hyeree Kim, Ichiro Manabe, Tomomi Nemoto, Kazuki Tainaka, Akinobu Hamada, Hjalmar Brismar, Etsuo A. Susaki

Department of Biochemistry: Faculty Publications

Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktopequipped SPIM for cleared specimens), a low-cost ($20,000–50,000), lowexpertise (one-day installation by a non-expert), yet practical do-it-yourself light-sheet microscope as a solution for this bottleneck. Even the most fundamental configuration of descSPIM enables multi-color imaging of whole mouse brains and a cancer cell line-derived xenograft tumor mass for the visualization of neurocircuitry, assessment of drug distribution, and pathological examination by false-colored hematoxylin and eosin staining in a threedimensional manner. Academically open-sourced (https://github.com/dbsbjuntendo/ …

Centering Relationality And Care For Stewardship Of Indigenous Research Data, Miranda Belarde-Lewis, Sandra Littletree, Iisaaksiichaa Ross Braine, Kaitlin Srader, Nestor Guerrero, Carole L. Palmer

Copyright, Fair Use, Scholarly Communication, etc.

The CARE Principles for Indigenous Data Governance are a seminal advance in the stewardship of Indigenous data. The Data Services for Indigenous Scholarship and Sovereignty (DSISS) project is working to guide how research libraries and data repositories can apply the CARE principles to support scholars of Indigenous culture and language. Building on a set of foundational case studies of Indigenous scholarship, this paper reports on analysis of formal engagement activities with scholars, Indigenous community members, and information and data professionals. We discuss three prominent themes—ownership, trust, and relational accountability—and their implications for concrete steps toward implementation of the CARE principles …

Investigating Nutrient Biomarkers Of Healthy Brain Aging: A Multimodal Brain Imaging Study, Christopher E. Zwilling, Jisheng Wu, Aron K. Barbey

Department of Psychology: Faculty Publications

The emerging field of Nutritional Cognitive Neuroscience aims to uncover specific foods and nutrients that promote healthy brain aging. Central to this effort is the discovery of nutrient profiles that can be targeted in nutritional interventions designed to promote brain health with respect to multimodal neuroimaging measures of brain structure, function, and metabolism. The present study therefore conducted one of the largest and most comprehensive nutrient biomarker studies examining multimodal neuroimaging measures of brain health within a sample of 100 older adults. To assess brain health, a comprehensive battery of well-established cognitive and brain imaging measures was administered, along with …

Impact Of The Department Of Defense Maternity Leave Policy Change On Retention And Postpartum Depression And The Air Force Postpartum Physical Fitness Policy Change On Physical Readiness Outcomes, Minette S. R. Herrick

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Understanding the implications of health policy is paramount to support continued execution and further wide-spread distribution. The Department of Defense has made many recent family friendly policy changes in effort to improve the health and morale of the force. Two such policies were analyzed in this study. The first was the change in maternity leave, in 2016, from allotting women six weeks time off to twelve weeks time off in the postpartum period. The second was executed by the Air Force to permit postpartum women a longer duration for their required physical fitness assessment (an increase from six months to …

Comprehensive In Vitro And In Vivo Evaluation Of Therapeutic Potential Of Bacopa-Derived Asiatic Acid Against A Human Oral Pathogen Streptococcus Mutans, Rajendran Jeyasri, Pandiyan Muthuramalingam, Arumugam Priya, Rajaiah Alexpandi, N. R. Siva Shanmugam, Saminathan Nivetha, Hyunsuk Shin, Shunmugiah Karutha Pandian, Arumugam Veera Ravi, Manikandan Ramesh

Food for Health: Publications

Dental caries is a common human oral disease worldwide, caused by an acid-producing bacteria Streptococcus mutans. The use of synthetic drugs and antibiotics to prevent dental caries has been increasing, but this can lead to severe side effects. To solve this issue, developing and developed countries have resorted to herbal medicines as an alternative to synthetic drugs for the treatment and prevention of dental caries. Therefore, there is an urgent need for plant-derived products to treat such diseases. Bacopa monnieri, a well-documented medicinal plant, contains 52 phytocompounds, including the pentacyclic triterpenoid metabolite known as asiatic acid (ASTA). Hence, …

Early Intervention Within Orthodontics: The Sociological Aspects, Trenton Hammond

Honors Program: Senior Projects (Public)

Orthodontic treatment is one of the final phases of dental treatment. Its use is to provide aesthetics in some cases, yet the quality-of-life aspect that it can provide is greatly underestimated. Orthodontic care can provide proper function of teeth, improve facial structure, avoid tooth decay/loss, and improve gum health (AAO, 2024). Within this research the overarching goal is to understand the sociological aspects of families looking to start orthodontic care, what the reasons behind starting or not starting are, and providing information to the public about orthodontics that may be misunderstood or might push people away from searching for orthodontic …

Unraveling The Role Of Lipid Droplets And Perilipin 2 In Bovine Luteal Cells, Michele R. Plewes, Heather A. Talbott, Micah B. Schott, Jennifer R. Wood, Andrea S. Cupp, John S. Davis

Department of Animal Science: Faculty Publications

Steroidogenic tissues contain cytosolic lipid droplets that are important for steroidogenesis. Perilipin 2 (PLIN2), a structural coat protein located on the surface of lipid droplets in mammalian cells, plays a crucial role in regulating lipid droplet formation and contributing to various cellular processes such as lipid storage and energy homeostasis. Herein, we examine the role that PLIN2 plays in regulating progesterone synthesis in the bovine corpus luteum. Utilizing gene array databases and Western blotting, we have delineated the expression pattern of PLIN2 throughout the follicular to luteal transition. Our findings reveal the presence of PLIN2 in both ovarian follicular and …

Investigating Small Drinking Water System Technical Capacity To Treat For Pfas, Chloe J. Yoder, Kaycie Lane

Department of Civil and Environmental Engineering: Faculty Publications

Per- and polyfluoroalkyl substances (PFAS) presents technical challenges in small systems where advanced drinking water treatment implementation is difficult. Publicly available data were used to examine technical treatment capacity for PFAS in the US, using Nebraska as a pilot. Of 1312 PWSs in Nebraska, 441 have technologies capable of removing PFAS from drinking water. Reverse osmosis was the most common treatment technology in Nebraska with 277 total systems using this technology with, 194 PWSs with RO serving populations of <= 100 people. Fifty-two PWSs had granular activated carbon, 47 had ion exchange and 62 had ultraviolet, with UV being primarily used for disinfection. We found PWSs had different technology deployment methods, several systems had “significant deficiencies” reported in management and operation evaluations, and age and previous water quality violations were not correlated to treatment evaluations. The developed methodology models utilizing publicly available data for contaminants across the US.

Cereal Rye Cover Crop Termination At Or Before Soybean Planting Has Minimal Effect On Soybean Yield Across The Midwestern Us, Tatiane Severo Silva, Spyridon Mourtzinis, Anthony Justin Mcmechan, Gabriela Inveninato Carmona, Bruce D. Potter, Kelley J. Tilmon, Louis S. Hesler, Nicholas J. Seiter, Robert Wright, Shannon L. Osborne, Thomas E. Hunt, Shawn P. Conley

Department of Entomology: Faculty Publications

Context: Interest is growing among farmers in the Midwestern US region in the potential benefits of establishing cereal rye (Secale cereale L.) as a cover crop. However, they are concerned about the implications and potential trade-offs due to this practice. Objective: We aimed to evaluate the effects of cereal rye cover crop termination at or before soybean planting on soybean (Glycine max [L.] Merr.) seed yield. Methods: Field experiments were conducted from 2019 to 2021 across 28 location-years in the Midwestern US. Treatments consisted of no cereal rye, cereal rye termination before soybean planting, and termination at planting. …

Analysis Of Deck-Building Games For The Development In A Digital Medium, Eric Ian Dundas

Honors Program: Senior Projects (Public)

This thesis discusses the game design justifications of Rift Colosseum, a deckbuilder card game developed by Eric Dundas. The research conducted intends to reinforce the design decisions for the game. The research includes systems from Trading Card Games Magic: The Gathering, the Pokémon Trading Card Game, and Flesh and Blood. Additionally, research is conducted on Slay the Spire, a deckbuilder roguelike. The analysis helped to find unique mechanics that could be utilized further when combined with other systems, especially digital-only mechanics inspired by Slay the Spire.

Several concluding materials were developed, such as the card types, card restrictions and …

Understanding Older People’S Voice Interactions With Smart Voice Assistants: A New Modified Rule-Based Natural Language Processing Model With Human Input, Zhengxu Yan, Victoria Dube, Judith Heselton, Kate Johnson, Changmin Yan, Valerie Jones, Julie Blaskewicz Boron, Marcia Shade

College of Journalism and Mass Communications: Faculty Publications

The COVID-19 pandemic has expedited the integration of Smart Voice Assistants (SVA) among older people. The qualitative data derived from user commands on SVA is pivotal for elucidating the engagement patterns of older individuals with such systems. However, the sheer volume of user-generated voice interaction data presents a formidable challenge for manual coding. Compounding this issue, age-related cognitive decline and alterations in speech patterns further complicate the interpretation of older users’ SVA voice interactions. Conventional dictionary-based textual analysis tools, which count word frequencies, are inadequate in capturing the evolving and communicative essence of these interactions that unfold over a series …

An Analysis Of Attending Industry Events As An Independent Game Developer: My Experience At The 2024 Game Developers Conference, Ben Laube

Honors Program: Senior Projects (Public)

The game development industry, known for its rapid evolution and competitive nature, presents significant challenges and opportunities for both novice and veteran developers. This paper provides a personal account and reflection on attending the 2024 Game Developers Conference (GDC) in San Francisco, aiming to demonstrate the substantial impact such events can have on independent developers. Through detailed descriptions of attended sessions and networking opportunities, including “GDC Pitch: Day 2,” “Getting Hired With a Personal Touch,” “Learn From Our Mistakes,” “Speed Networking,” and the “GDC Expo” this paper explores the value of industry events in terms of knowledge gained, connections made, …