Digital Commons Network^™

Assessment Of Foam Decontamination In Tomato Washing Process, Basim M. Alohali

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

The work of the dissertation investigated the efficacy of foam-based decontamination treatments to enhance microbial safety in Roma tomatoes, specifically targeting natural microflora and Salmonella contamination. Growing concerns over foodborne illnesses linked to fresh tomatoes and the limitations of conventional decontamination techniques, such as organic matter built-up and limited microbial reduction, are addressed. The primary objectives were to characterize the physiochemical properties of foams formed using various concentrations of Tween 20 (T20) and sodium dodecyl sulfate (SDS) with and without the addition of sodium hypochlorite (NaOCl) and peracetic acid (PAA), compare the efficacy of foam and traditional dump tank (DT) …

Manipulations Of The Caenorhabditis Elegans Microbiome Reveal Influences Of Detrimental Members On Host Life History Traits, Ashley Elizabeth Foltz

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Microbiomes are a fundamental part of eukaryotic organisms and play a role in both host health and fitness. Although microbiomes are often associated with health and positive effects on the host, certain microbiome compositions are also associated with disease. We used Caenorhabditis elegans and its experimental microbiome, CeMbio to explore the interactions that individual microbiome members have with other microbes, with the host, and how the entire microbiome community interacts with the host. We found that individual CeMbio members have varying effects on host health and fitness compared to the whole community. This suggested that there is synergy occurring in …

Development Of A Durable And Low-Dose Universal Vaccine Against Swine Influenza A Virus, Erika Petro-Turnquist

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Swine Influenza A Virus (IAV-S) imposes a significant burden on the pork industry and poses a great threat to public health due to its zoonotic potential. Susceptibility to human, avian, and swine influenza make pigs the ultimate mixing vessels for reassorted influenza A virus strains. Current vaccination strategies induce short-lived and strain-specific responses against IAV-S. Further, the substantial genetic diversity of IAV-S necessitates consistent revaccination and updating of commercial vaccines. Computational platforms have recently emerged as a promising strategy to develop new-age vaccines against infectious diseases in livestock animals. Here, we describe a computationally derived set of vaccine immunogens, called …

Estranged Temporality: How Time Tells Stories In Science Fiction, Phillip H. Howells

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

According to Darko Suvin in his influential critical treatise, Metamorphoses of Science Fiction, estrangement in Science Fiction (SF) gives authors the ability to build worlds related to but distant from our own using specific metaphors. This dissertation takes up this term and applies it to the fulcrum of time in SF as a method of creating possible futures and imaginative pasts in order to illuminate the realities of the present. The realities of the present are congruent with the material circumstances of the past, and this can be seen in the kinds of SF worlds built by members of …

Spatial Ecology Of Mountain Lions Throughout Their Distribution In California, Kyle Duncan Dougherty

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Mountain lions (Puma concolor) have been studied extensively throughout their wide geographic distribution in the Western Hemisphere, but most research has been conducted at relatively small spatial scales. We used GPS telemetry data contributed by researchers in 16 study areas throughout California to advance understanding of mountain lion spatial ecology. First, we investigated multi-scale resource selection of mountain lions relative to spatially varying risk of intentional human-caused mortality associated with livestock depredation management. We show that individuals that proactively avoided mortality risk when selecting home ranges were able to relax their avoidance of risk when making resource selection …

Genetically Modified Bovine Milk-Derived Small Extracellular Vesicles In Drug Delivery, Alice Kah Hui Ngu

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Small extracellular vesicles (SEVs, “exosomes”) are natural nanoparticles that can be endogenously synthesized and also obtained from dietary sources, such as bovine milk SEVs (mEVs). mEVs have been extensively studied and discovered with contributive features that potentially play a role in drug delivery and other therapeutic purposes, such as diagnostic tools. Current studies have shown that mEVs have been used preclinically to carry in targeting drugs, nucleotides, biomolecules, and labels to target metabolic diseases and cancers. Extensive studies have focused on further modifying SEVs to enhance their versatility in pharmaceutical use. In this dissertation, we exhibited one of the ways …

Advancements In Microscale Tensile Testing: A Novel Device For The High Throughput Analysis Of Two-Photon Polymerized Microfibers In Liquid, Grayson D. Minnick

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Two-photon polymerization (TPP) enables the 3D fabrication of micro- and nanostructures from biocompatible materials with high resolutions, reaching into the realm of hundreds of nanometers. Such capabilities are crucial for advancing applications in fields like mechanobiology. As microscale technologies evolve, the precise characterization of material properties becomes increasingly essential. This is especially true for TPP-printed microstructures serving as scaffolds for 3D cell culture and intended for studying the mechanics of tissues. Despite the importance, traditional micro-scale tensile testing methods typically lack scalability and fail to replicate relevant environmental conditions. This dissertation introduces a new device that is specifically designed to …

Risk Factors For Online And In-Person Stalking, Intrusive Harassment, And Violence Within A Community Sample, Elisha J. C. Chan

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Stalking and intrusive harassment (SIH) and stalking violence are prevalent and serious public health concerns associated with significant physical, psychological, financial, and social consequences. The growing proliferation of technology and the internet has only exacerbated the problem as SIH behaviors can be easily perpetrated via electronic means. Given its prevalence and negative impact, it is imperative to investigate the risk factors for online and in-person SIH and stalking violence to better predict and mitigate its detrimental effects. The current study therefore aimed to 1) examine the descriptive characteristics associated with online and in-person SIH and stalking violence; and 2) identify …

Virtual Fencing In Livestock Management: Effects On Beef Cattle Stress And Enhancing Wildlife Conservation, Kaitlyn E. Dozler

School of Natural Resources: Dissertations, Theses, and Student Research

I examined the effects of virtual fencing on beef cattle stress and how implementing the technology can aid wildlife habitat conservation at the University of Nebraska Gudmundsen Sandhills laboratory (GSL) near Whitman, NE. First, I examined how virtual fencing influences acute heart rate changes in beef cattle fitted with the virtual fencing collars. No significant difference in the average heart rate of a cow (P > 0.05) was observed between the 30 min control and virtual fenced periods for both trial dates. Second, I examined the influence of topography on wildlife species richness and presence at GSL using wildlife camera …

Spatial Ecology Of Mountain Lions Throughout Their Distribution In California, Kyle D. Dougherty

School of Natural Resources: Dissertations, Theses, and Student Research

Mountain lions (Puma concolor) have been studied extensively throughout their wide geographic distribution in the Western Hemisphere, but most research has been conducted at relatively small spatial scales. We used GPS telemetry data contributed by researchers in 16 study areas throughout California to advance understanding of mountain lion spatial ecology. First, we investigated multi-scale resource selection of mountain lions relative to spatially varying risk of intentional human-caused mortality associated with livestock depredation management. We show that individuals that proactively avoided mortality risk when selecting home ranges were able to relax their avoidance of risk when making resource selection …

My Ai Companion: An Examination Of The Removal Of Erotic Role Play From Replika Through User Discussion On Reddit, Chelsee M. Allen

Department of Sociology: Dissertations, Theses, and Student Research

The development of artificial intelligence (AI) software has expanded rapidly in recent years, and thus has emerged the importance of exploring human relationships with AI chatbots. Replika, an app which uses AI to mimic human conversation, removed a function called Erotic Role Play (ERP) that allowed for sexual conversation with users’ customizable chatbots in February of 2023. This exploratory qualitative study examines the aftermath of ERP’s removal through an analysis of user interactions on Reddit. Five overarching themes emerged through the analysis of top posts to a Replika-specific subreddit, encompassing topics around mental health, stigma, coping, sex work and gendered …

Structural Analysis Of Dj-1 Glyoxalase Activity By Mix-And-Inject Serial Synchrotron Crystallography, Coleman Dolamore

Department of Biochemistry: Dissertations, Theses, and Student Research

Observing enzyme structures while they are catalyzing a reaction has been a major goal of both enzymology and structural biology for decades. With the advent of time-resolved serial X-ray crystallography using X-ray free electron lasers (XFELs) and synchrotron sources, enzyme reactions can be monitored in real time in crystallo. Here, we use two different approaches to study related enzymes involved in methylglyoxal detoxification. For human DJ-1, we used pink beam mix-and-inject serial crystallography (MISC) at the Advanced Photon Source (BioCARS 14-ID) to probe the controversial mechanism of DJ-1’s action on methylglyoxal by using serial Laue diffraction. The high flux …

Data-Driven Drug Repurposing For Immune System-Related Diseases, Sabyasachi Mohanty

Department of Biochemistry: Dissertations, Theses, and Student Research

In this research, we explore a data-driven approach for drug repurposing to enhance the immunomodulatory effect by integrating pattern-based search and genome-scale metabolic modeling. This research helps to find a solution to the preexisting problems of drug discovery which includes expenses and a high amount of time consumption. By leveraging the pre-existing data of the approved drugs to identify the major metabolic pathway changes, we can find new drugs with similar effects with less off-target effects.

The focus on the immune system modulating drugs is due to the high prevalence of immune system-involved diseases and the growing demand for effective …

Enhancing Water Sustainability In North Africa: Literature Review And Synthesis Of Current Knowledge Gaps In Sudan, Osman M. A. Adam, Aaron R. Mittelstet, Derek M. Heeren, Troy E. Gilmore

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

This study delves into the critical role of groundwater in addressing global water challenges, with a focus on the Nubian Sandstone Aquifer System (NSAS) in North Africa. Groundwater constitutes a source of potable water, irrigation, and industrial use, especially in arid regions where surface water is limited. We analyzed the status of water quantity, withdrawals, recharge, and geological characteristics in the NSAS, specifically in Sudan, Egypt, Libya, and Chad. Though the NSAS is largely an untapped resource, we evaluated various scenarios to determine the quantity of cropland that can be sustainably irrigated. The NSAS is located in an arid region, …

Investigating The Abilities Of Potentially Beneficial Bacteria For Increasing Nitrogen-Use Efficiency In Maize, Lexie R. Foster

School of Biological Sciences: Dissertations, Theses, and Student Research

Global agriculture relies heavily on the use of synthetic nitrogen fertilizer to meet the current global food demand. Unfortunately, the average nitrogen-use efficiency (NUE) of maize (Zea mays) is approximately 50 percent. Improving the NUE of maize is essential for meeting the growing global food demand while also decreasing the negative environmental impacts caused by losses of nitrogen fertilizer due to runoff and volatilization. Harnessing the symbiotic relationship between plants and the soil microbiome may be one method for increasing the NUE in crops such as maize. In the present study, a set of potentially beneficial bacterial species …

Archaeoentomology: Arthropods And Humans, Current Works In Archaeoentomology, Paz Vigil

Department of Entomology: Distance Master of Science Projects

This paper presents a brief literature review, exploring human and insect interaction, and how such research aids the ongoing construction of the archaeological and entomological record. This field, a combined subset of both entomology and archaeology, is both complex and rich as well as young and still growing. This literature review is not meant to be an exhaustive or comprehensive exploration of this subject, but it will serve as part of a foundation for those interested in the topic or wanting to conduct further exploratory study in this field. The intent is to highlight the practical applications of Archaeoentomology, using …

Heat Stress Changes The Bovine Methylome And Transcriptome And Investigation Of Two Novel Genetic Defects In Cattle, Rachel Renae Reith

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Heat stress is a major concern for livestock producers due to its negative impact on animal health and productivity. Heat stress does so by altering expression of genes through different regulatory mechanisms such as DNA methylation. Understanding how heat stress alters gene expression will help elucidate the genetic basis of physiological changes as well as identify targets for possible heat stress mitigation. The purpose of the first study was to understand how heat stress alters the adipose and skeletal muscle transcriptomes in zilpaterol-fed Brahman, as zilpaterol improves muscle growth and may mitigate the effects of heat stress. Differential expression and …

Exploring The Associations Of Afterschool Science Participation And Friendships With Science Identities, Patricia Wonch Hill, Grace M. Kelly, Julia Mcquillan, Jorge Ledesma, Miranda Melson, G. Robin Gauthier

Worlds of Connections Resources

Building on previous research that demonstrates the association of youth experiences in afterschool science and higher science identities, this paper presents a network study of 421 middle school students that examines afterschool science participation, friendship ties, and science identities. Participation in afterschool science clubs is associated with higher science identity, but the mechanisms and order of causality are unclear. Youth form friendships inside and outside of school, and peers may influence participation in afterschool activities, as empirical research on friendships shows that they are associated with youth interests. These peer interactions also have the potential to shape identity development during …

Evaluation Of An Autonomous Acoustic Surveying Technique For Grassland Bird Communities In Nebraska, Grace E. Schuster, Leroy J. Walston, Andrew R. Little

School of Natural Resources: Faculty Publications

Monitoring trends in wildlife communities is integral to making informed land management decisions and applying conservation strategies. Birds inhabit most niches in every environment and because of this they are widely accepted as an indicator species for environmental health. Traditionally, point counts are the common method to survey bird populations, however, passive acoustic monitoring approaches using autonomous recording units have been shown to be cost-effective alternatives to point count surveys. Advancements in automatic acoustic classification technologies, such as BirdNET, can aid in these efforts by quickly processing large volumes of acoustic recordings to identify bird species. While the utility of …

Daily Eicosapentaenoic Acid Infusion In Iugr Fetal Lambs Reduced Systemic Inflammation, Increased Muscle Adrβ2 Content, And Improved Myoblast Function And Muscle Growth, Haley Beer, Taylor A. Lacey, Rachel L. Gibbs, Micah S. Most, Zena M. Hicks, Pablo C. Grijalva, Eileen S. Marks-Nelson, Ty B. Schmidt, Jessica Lynn Petersen, Dustin T. Yates

Department of Animal Science: Faculty Publications

Intrauterine growth-restricted (IUGR) fetuses exhibit systemic inflammation that contributes to programmed deficits in myoblast function and muscle growth. Thus, we sought to determine if targeting fetal inflammation improves muscle growth outcomes. Heat stress-induced IUGR fetal lambs were infused with eicosapentaenoic acid (IUGR+EPA; n = 9) or saline (IUGR; n = 8) for 5 days during late gestation and compared to saline-infused controls (n = 11). Circulating eicosapentaenoic acid was 42% less (p < 0.05) for IUGR fetuses but was recovered in IUGR+EPA fetuses. The infusion did not improve placental function or fetal O2 but resolved the 67% greater (p < 0.05) circulating TNFα observed in IUGR fetuses. This improved myoblast function and muscle growth, as the 23% reduction (p < 0.05) in the ex vivo differentiation of IUGR myoblasts was resolved in IUGR+EPA myoblasts. Semitendinosus, longissimus dorsi, and flexor digitorum superficialis muscles were 24–39% lighter (p < 0.05) for IUGR but not for IUGR+EPA fetuses. Elevated (p < 0.05) IL6R and reduced (p < 0.05) β2 adrenoceptor content in IUGR muscle indicated enhanced inflammatory sensitivity and diminished β2 adrenergic sensitivity. Although IL6R remained elevated, β2 adrenoceptor deficits were resolved in IUGR+EPA muscle, demonstrating a unique underlying mechanism for muscle dysregulation. These findings show that fetal inflammation contributes to IUGR muscle growth deficits and thus may be an effective target for intervention.

Nebraska Farm Real Estate Market Highlights 2023-2024, Jim Jansen, Jeff Stokes

Nebraska Farm Real Estate Reports

For the fifth consecutive year, the all-land average value in Nebraska rose for the year ending February 1, 2024, averaging approximately 5% higher than the prior year. Figure 2 summarizes these figures and trends along with the percent changes over the preceding year’s all-land average for the eight districts in the state.

The statewide all-land average value for the year ending February 1, 2024, averaged $4,015 per acre, or about a 5% ($180 per acre) increase from the prior year’s value of $3,835 per acre. Rates of increase were the highest in the North and Northeast Districts, as these areas …

Perspectives On Computational Modeling Of Biological Systems And The Significance Of The Sysmod Community, Bhanwar Lal Puniya, Meghna Verma, Chiara Damiani, Shaimaa Bakr, Andreas Dräger

Department of Biochemistry: Faculty Publications

Motivation: In recent years, applying computational modeling to systems biology has caused a substantial surge in both discovery and practical applications and a significant shift in our understanding of the complexity inherent in biological systems.

Results: In this perspective article, we briefly overview computational modeling in biology, highlighting recent advancements such as multiscale modeling due to the omics revolution, single-cell technology, and integration of artificial intelligence and machine learning approaches. We also discuss the primary challenges faced: integration, standardization, model complexity, scalability, and interdisciplinary collaboration. Lastly, we highlight the contribution made by the Computational Modeling of Biological Systems …

Sensitization Of Europium Oxide Nanoparticles Enhances Signal-To- Noise Over Autofluorescence With Time-Gated Luminescence Detection, Hunter Miller, Jessica Q. Wallace, Hui Li, Xing-Zhong Li, Ana De Bettencourt-Dias, Forrest Kievit

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Clinical translation of nanoparticle-based therapeutics has been limited, and a lack of preclinical delivery characterization is partly to blame, limiting our understanding of the mechanisms of failure. The improvement of the preclinical delivery assessment requires nanoparticles with higher detectability. This work focused on the exploration of several aromatic carboxylic ligands (terephthalic acid, quinaldic acid, and kynurenic acid) for the sensitization of europium oxide nanoparticles with a long emission lifetime to overcome cellular autofluorescence, a key confounder of detection in luminescence-based bioimaging. A facile one-pot synthesis and ligand exchange process generated and sensitized ultrasmall Eu₂O₃ cores. As reflected …

A Comprehensive Study On The Impact Of Human Hair Fiber And Millet Husk Ash On Concrete Properties: Response Surface Modeling And Optimization, Naraindas Bheel, Muhammad Alamgeer Shams, Samiullah Sohu, Abdul Salam Buller, Taoufik Najeh, Fouad Ismail Ismail, Omrane Benjeddou

Department of Civil and Environmental Engineering: Faculty Publications

Revolutionizing construction, the concrete blend seamlessly integrates human hair (HH) fibers and millet husk ash (MHA) as a sustainable alternative. By repurposing human hair for enhanced tensile strength and utilizing millet husk ash to replace sand, these materials not only reduce waste but also create a durable, eco-friendly solution. This groundbreaking methodology not only adheres to established structural criteria but also advances the concepts of the circular economy, representing a significant advancement towards environmentally sustainable and resilient building practices. The main purpose of the research is to investigate the fresh and mechanical characteristics of concrete blended with 10–40% MHA as …

From Australia To Nebraska: Researching Social Value, Marilyn R. Schlake, Cheryl A. Burkhart-Kriesel, Jean Ann Fischer, Michelle Krehbiel, Randy Saner

Cornhusker Economics

Globally, there is a growing body of research on social capital through the lens of social value, or how the social connections translate into actions with tangible outcomes impacting livelihoods and well-being. In the United States, social value is a relatively new perspective that will add to the social capital discussion.

Concepts In Animal Parasitology, Scott L. Gardner, Sue Ann Gardner

Zea E-Books Collection

Note that this version of the textbook has been superceded by the one updated in 2026, available at the UNL Pressbooks site.

The hard copy version that is for sale at lulu.com is the old version.

"This is a textbook covering concepts in animal parasitology. It is meant to be used by students, teachers, professors, researchers, and members of the public who are interested in learning about animal parasite biology, systematics, taxonomy, zoogeography, and ecology. The primary intended audience is upper-level undergraduate or graduate university students who have knowledge of basic biology and, particularly, basic animal biology. (863 pages, …

Protein Nanoparticles As Vaccine Platforms For Human And Zoonotic Viruses, Kush Kumar Pandey, Bikash R. Sahoo, Asit K. Pattnaik

Nebraska Center for Virology: Faculty Publications

Vaccines are one of the most effective medical interventions, playing a pivotal role in treating infectious diseases. Although traditional vaccines comprise killed, inactivated, or liveattenuated pathogens that have resulted in protective immune responses, the negative consequences of their administration have been well appreciated. Modern vaccines have evolved to contain purified antigenic subunits, epitopes, or antigen-encoding mRNAs, rendering them relatively safe. However, reduced humoral and cellular responses pose major challenges to these subunit vaccines. Protein nanoparticle (PNP)-based vaccines have garnered substantial interest in recent years for their ability to present a repetitive array of antigens for improving immunogenicity and enhancing protective …

Tsunami Exposure And Mental Health Consequences: Protective Role Of Cultural Coping Strategies, Thulitha Wickrama, Michael J. Merten, K. A.S. Wickrama, Amanda Terrell

Department of Child, Youth, and Family Studies: Faculty Publications

There is a knowledge gap regarding the link between disaster exposure and adolescent mental health problems in developing countries. This study examines the case of Sri Lanka to investigate (a) the immediate and long-term mental health impact of the 2004 tsunami disaster on adolescents and (b) the potential moderating effects of unique cultural and family practices that prevail in Sri Lanka. This study used a random sample of 160 adolescents (ages 12–19) and their mothers who were exposed to the tsunami disaster while living in a southern Sri Lankan village and provided prospective data immediately after the disaster (2005) and …

Sustainable Sensing With Paper Microfluidics: Applications In Health, Environment, And Food Safety, Sanjay Kumar, Jyoti B. Kaushal, Heow Pueh Lee

Durham School of Architectural Engineering and Construction: Faculty Publications

This manuscript offers a concise overview of paper microfluidics, emphasizing its sustainable sensing applications in healthcare, environmental monitoring, and food safety. Researchers have developed innovative sensing platforms for detecting pathogens, pollutants, and contaminants by leveraging the paper’s unique properties, such as biodegradability and affordability. These portable, low-cost sensors facilitate rapid diagnostics and on-site analysis, making them invaluable tools for resource-limited settings. This review discusses the fabrication techniques, principles, and applications of paper microfluidics, showcasing its potential to address pressing challenges and enhance human health and environmental sustainability.

Violent Conflict Drives World Hunger And Food Insecurity, E. Wesley F. Peterson

Cornhusker Economics

The World Food Program (WFP) with support from the United States and other countries works to provide food to those in need as a result of conflict, adverse climate effects, poverty, and government mismanagement. It is limited in what it can do, however, by budget constraints and rising food prices. On top of the financial issues, the delivery of food assistance is particularly complicated in areas of conflict where warring adversaries often hijack food supplies intended for the other side. While it is illegal under International Humanitarian Law to use starvation as a weapon of war, Dzerowicz (2023) has identified …