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Il-10 As A Th2 Cytokine: Differences Between Mice And Humans, Mahima T. Rasquinha, Meghna Sur, Ninaad Lasrado, Jay Reddy Jan 2021

Il-10 As A Th2 Cytokine: Differences Between Mice And Humans, Mahima T. Rasquinha, Meghna Sur, Ninaad Lasrado, Jay Reddy

School of Veterinary and Biomedical Sciences: Faculty Publications

The discovery of interleukin (IL)-10 more than 30 years ago marked the beginning of our understanding of how cytokines regulate immune responses, based on cross-regulation between T helper (Th)1 and Th2 cytokines. Although multiple cell types were shown to produce IL-10, its identity as a Th2 cytokine remained strong since it was rigidly associated with Th2 clones in mice, whereas both Th1 and Th2 clones could secrete IL-10 in humans. However, as new Th1/Th2 cell functionalities emerged, anti-inflammatory action of IL-10 gained more attention than its inhibitory effect on Th1 cells, which may occur as an indirect consequence of suppression …


Terrestrial Ecological Risk Analysis Via Dietary Exposure At Uranium Mine Sites In The Grand Canyon Watershed (Arizona, Usa), Jo Ellen Hinck, Danielle Cleveland, Bradley E. Sample Jan 2021

Terrestrial Ecological Risk Analysis Via Dietary Exposure At Uranium Mine Sites In The Grand Canyon Watershed (Arizona, Usa), Jo Ellen Hinck, Danielle Cleveland, Bradley E. Sample

United States Geological Survey: Staff Publications

The U.S. Department of the Interior recently included uranium (U) on a list of mineral commodities that are considered critical to economic and national security. The uses of U for commercial and residential energy production, defense applications, medical device technologies, and energy generation for space vehicles and satellites are known, but the environmental impacts of uranium extraction are not always well quantified. We conducted a screening-level ecological risk analysis based on exposure to miningrelated elements via diets and incidental soil ingestion for terrestrial biota to provide context to chemical characterization and exposures at breccia pipe U mines in northern Arizona. …


Managing For Radical Ecosystem Change: Applying The Resist-Accept- Direct (Rad) Framework, Abigail J. Lynch, Laura M. Thompson, Erik A. Beever, David N. Cole, Augustin C. Engman, Cat Hawkins Hoffman, Stephen T. Jackson, Trevor J. Krabbenhoft, David J. Lawrence, Douglas Limpinsel, Robert T. Magill, Tracy A. Melvin, John M. Morton, Robert A. Newman, Jay O. Peterson, Mark T. Porath, Frank J. Rahel, Gregor W. Schuurman, Suresh A. Sethi, Jennifer L. Wilkening Jan 2021

Managing For Radical Ecosystem Change: Applying The Resist-Accept- Direct (Rad) Framework, Abigail J. Lynch, Laura M. Thompson, Erik A. Beever, David N. Cole, Augustin C. Engman, Cat Hawkins Hoffman, Stephen T. Jackson, Trevor J. Krabbenhoft, David J. Lawrence, Douglas Limpinsel, Robert T. Magill, Tracy A. Melvin, John M. Morton, Robert A. Newman, Jay O. Peterson, Mark T. Porath, Frank J. Rahel, Gregor W. Schuurman, Suresh A. Sethi, Jennifer L. Wilkening

United States Geological Survey: Staff Publications

Ecosystem transformation involves the emergence of persistent ecological or social–ecological systems that diverge, dramatically and irreversibly, from prior ecosystem structure and function. Such transformations are occurring at increasing rates across the planet in response to changes in climate, land use, and other factors. Consequently, a dynamic view of ecosystem processes that accommodates rapid, irreversible change will be critical for effectively conserving fish, wildlife, and other natural resources, and maintaining ecosystem services. However, managing ecosystems toward states with novel structure and function is an inherently unpredictable and difficult task. Managers navigating ecosystem transformation can benefit from considering broader objectives, beyond a …


Convolutional Neural Networks Can Decode Eye Movement Data: A Black Box Approach To Predicting Task From Eye Movements, Zachary J. Cole, Karl M. Kuntzelman, Michael D. Dodd, Matthew R. Johnson Jan 2021

Convolutional Neural Networks Can Decode Eye Movement Data: A Black Box Approach To Predicting Task From Eye Movements, Zachary J. Cole, Karl M. Kuntzelman, Michael D. Dodd, Matthew R. Johnson

Department of Psychology: Faculty Publications

Previous attempts to classify task from eye movement data have relied on model architectures designed to emulate theoretically defined cognitive processes and/or data that have been processed into aggregate (e.g., fixations, saccades) or statistical (e.g., fixation density) features. Black box convolutional neural networks (CNNs) are capable of identifying relevant features in raw and minimally processed data and images, but difficulty interpreting these model architectures has contributed to challenges in generalizing lab-trained CNNs to applied contexts. In the current study, a CNN classifier was used to classify task from two eye movement datasets (Exploratory and Confirmatory) in which participants searched, memorized, …


Social Connectedness And Negative Affect Uniquely Explain Individual Differences In Response To Emotional Ambiguity, Maital Neta, Rebecca L. Brock Jan 2021

Social Connectedness And Negative Affect Uniquely Explain Individual Differences In Response To Emotional Ambiguity, Maital Neta, Rebecca L. Brock

Department of Psychology: Faculty Publications

Negativity bias is not only central to mood and anxiety disorders, but can powerfully impact our decision-making across domains (e.g., financial, medical, social). This project builds on previous work examining negativity bias using dual-valence ambiguity. Specifically, although some facial expressions have a relatively clear negative (angry) or positive valence (happy), surprised expressions are interpreted negatively by some and positively by others, providing insight into one’s valence bias. Here, we examine putative sources of variability that distinguish individuals with a more negative versus positive valence bias using structural equation modeling. Our model reveals that one’s propensity toward negativity (operationalized as temperamental …


Much Ado About Missingness: A Demonstration Of Full Information Maximum Likelihood Estimation To Address Missingness In Functional Magnetic Resonance Imaging Data, Timothy D. Nelson, Rebecca L. Brock, Sonja Yokum, Cara C. Tomaso, Cary R. Savage, Eric Stice Jan 2021

Much Ado About Missingness: A Demonstration Of Full Information Maximum Likelihood Estimation To Address Missingness In Functional Magnetic Resonance Imaging Data, Timothy D. Nelson, Rebecca L. Brock, Sonja Yokum, Cara C. Tomaso, Cary R. Savage, Eric Stice

Department of Psychology: Faculty Publications

The current paper leveraged a large multi-study functional magnetic resonance imaging (fMRI) dataset (N = 363) and a generated missingness paradigm to demonstrate different approaches for handling missing fMRI data under a variety of conditions. The performance of full information maximum likelihood (FIML) estimation, both with and without auxiliary variables, and listwise deletion were compared under different conditions of generated missing data volumes (i.e., 20, 35, and 50%). FIML generally performed better than listwise deletion in replicating results from the full dataset, but differences were small in the absence of auxiliary variables that correlated strongly with fMRI task data. However, …


Skills-Based Intervention To Enhance Collaborative Decision-Making: Systematic Adaptation And Open Trial Protocol For Veterans With Psychosis, Emily B.H> Treichler, Borsika A. Rabin, William D. Spaulding, Michael L. Thomas, Michelle P. Salyers, Eric L. Granholm, Amy N. Cohen, Gregory A. Light Jan 2021

Skills-Based Intervention To Enhance Collaborative Decision-Making: Systematic Adaptation And Open Trial Protocol For Veterans With Psychosis, Emily B.H> Treichler, Borsika A. Rabin, William D. Spaulding, Michael L. Thomas, Michelle P. Salyers, Eric L. Granholm, Amy N. Cohen, Gregory A. Light

Department of Psychology: Faculty Publications

Background: Collaborative decision-making is an innovative decision-making approach that assigns equal power and responsibility to patients and providers. Most veterans with serious mental illnesses like schizophrenia want a greater role in treatment decisions, but there are no interventions targeted for this population. A skills-based intervention is promising because it is well-aligned with the recovery model, uses similar mechanisms as other evidence-based interventions in this population, and generalizes across decisional contexts while empowering veterans to decide when to initiate collaborative decision-making. Collaborative Decision Skills Training (CDST) was developed in a civilian serious mental illness sample and may fill this gap …


High Prevalence Of Pre-Existing Serological Cross-Reactivity Against Severe Acute Respiratory Syndrome Coronavirus-2 (Sars- Cov-2) In Sub-Saharan Africa, For Yue Tso, Salum Lidenge, Phoebe B. Peña, Ashley A. Clegg, John R. Ngowi, Julius Mwaiselage, Owen Ngalamika, Peter Julius, John T. West, Charles Wood Jan 2021

High Prevalence Of Pre-Existing Serological Cross-Reactivity Against Severe Acute Respiratory Syndrome Coronavirus-2 (Sars- Cov-2) In Sub-Saharan Africa, For Yue Tso, Salum Lidenge, Phoebe B. Peña, Ashley A. Clegg, John R. Ngowi, Julius Mwaiselage, Owen Ngalamika, Peter Julius, John T. West, Charles Wood

Nebraska Center for Virology: Faculty Publications

Objective: Significant morbidity and mortality have occurred in the USA, Europe, and Asia due to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), whereas the numbers of infections and deaths in sub-Saharan Africa (SSA) have remained comparatively low. It has been hypothesized that exposure of the population in SSA to other coronaviruses prior to the COVID-19 pandemic resulted in some degree of cross-protection against SARS-CoV-2 infection and pathogenesis. We evaluated this hypothesis by comparing SARS-CoV-2 cross-reactive antibodies in pre-pandemic plasma samples collected from SSA and the USA.

Method: Pre-COVID-19 pandemic plasma samples from SSA and the USA were collected and tested by …


The 20th Anniversary Meeting Of The Rocky Mountain Virology Association, Joel Rovnak, Laura A. St Clair, Carley Mcalister, Chinemerem P. Ogbu, Jessica Smolenske, Randall J. Cohrs, Rushika Perera Jan 2021

The 20th Anniversary Meeting Of The Rocky Mountain Virology Association, Joel Rovnak, Laura A. St Clair, Carley Mcalister, Chinemerem P. Ogbu, Jessica Smolenske, Randall J. Cohrs, Rushika Perera

Nebraska Center for Virology: Faculty Publications

Due to the COVID-19 pandemic and multiple devastating forest fires, the 2020 meeting of the Rocky Mountain Virology Association was held virtually. The three-day gathering featured talks describing recent advances in virology and prion research. The keynote presentation described the measles virus paradox of immune suppression and life-long immunity. Special invited speakers presented information concerning visualizing antiviral effector cell biology in mucosal tissues, uncovering the T-cell tropism of Epstein-Barr virus type 2, a history and current survey of coronavirus spike proteins, a summary of Zika virus vaccination and immunity, the innate immune response to flavivirus infections, a discussion concerning prion …


Chlorovirus Pbcv-1 Multidomain Protein A111/114r Has Three Glycosyltransferase Functions Involved In The Synthesis Of Atypical N-Glycans, Eric Noel, Anna Notaro, Immacolata Speciale, Garry A. Duncan, Cristina De Castro, James L. Van Etten Jan 2021

Chlorovirus Pbcv-1 Multidomain Protein A111/114r Has Three Glycosyltransferase Functions Involved In The Synthesis Of Atypical N-Glycans, Eric Noel, Anna Notaro, Immacolata Speciale, Garry A. Duncan, Cristina De Castro, James L. Van Etten

Nebraska Center for Virology: Faculty Publications

The structures of the four N-linked glycans from the prototype chlorovirus PBCV-1 major capsid protein do not resemble any other glycans in the three domains of life. All known chloroviruses and antigenic variants (or mutants) share a unique conserved central glycan core consisting of five sugars, except for antigenic mutant virus P1L6, which has four of the five sugars. A combination of ge- netic and structural analyses indicates that the protein coded by PBCV-1 gene a111/114r, conserved in all chloroviruses, is a glycosyltransferase with three putative domains of approximately 300 amino acids each. Here, in addition to in silico sequence …


Strategies Targeting Hemagglutinin As A Universal Influenza Vaccine, Brianna L. Bullard, Eric A. Weaver Jan 2021

Strategies Targeting Hemagglutinin As A Universal Influenza Vaccine, Brianna L. Bullard, Eric A. Weaver

Nebraska Center for Virology: Faculty Publications

Influenza virus has significant viral diversity, both through antigenic drift and shift, which makes development of a vaccine challenging. Current influenza vaccines are updated yearly to in- clude strains predicted to circulate in the upcoming influenza season, however this can lead to a mismatch which reduces vaccine efficacy. Several strategies targeting the most abundant and im- munogenic surface protein of influenza, the hemagglutinin (HA) protein, have been explored. These strategies include stalk-directed, consensus-based, and computationally derived HA immunogens. In this review, we explore vaccine strategies which utilize novel antigen design of the HA protein to improve cross-reactive immunity for development …


Zika Virus Infection Induces Endoplasmic Reticulum Stress And Apoptosis In Placental Trophoblasts, Philma Glora Muthuraj, Prakask K. Sahoo, Madison Kraus, Taylor Bruett, Arun S. Annamalai, Aryamav Pattnaik, Asit K. Pattnaik, Siddappa N. Byrareddy, Sathish Kumar Natarajan Jan 2021

Zika Virus Infection Induces Endoplasmic Reticulum Stress And Apoptosis In Placental Trophoblasts, Philma Glora Muthuraj, Prakask K. Sahoo, Madison Kraus, Taylor Bruett, Arun S. Annamalai, Aryamav Pattnaik, Asit K. Pattnaik, Siddappa N. Byrareddy, Sathish Kumar Natarajan

Nebraska Center for Virology: Faculty Publications

Zika virus (ZIKV) infection to a pregnant woman can be vertically transmitted to the fetus via the placenta leading to Congenital Zika syndrome. This is characterized by microcephaly, retinal defects, and intrauterine growth retardation. ZIKV induces placental trophoblast apoptosis leading to severe abnormalities in the growth and development of the fetus. However, the molecular mechanism behind ZIKV-induced apoptosis in placental trophoblasts remains unclear. We hypothesize that ZIKV infection induces endoplasmic reticulum (ER) stress in the trophoblasts, and sustained ER stress results in apoptosis. HTR-8 (HTR-8/SVneo), a human normal immortalized trophoblast cell and human choriocarcinoma-derived cell lines (JEG-3 and JAR) were …


Antiretroviral Therapy For Hiv-Associated Cutaneous Kaposi’S Sarcoma: Clinical, Hiv-Related, And Sociodemographic Predictors Of Outcome, Owen Ngalamika, Sody Munsaka, Salum J. Lidenge, John T. West, Charles Wood Jan 2021

Antiretroviral Therapy For Hiv-Associated Cutaneous Kaposi’S Sarcoma: Clinical, Hiv-Related, And Sociodemographic Predictors Of Outcome, Owen Ngalamika, Sody Munsaka, Salum J. Lidenge, John T. West, Charles Wood

Nebraska Center for Virology: Faculty Publications

Kaposi’s sarcoma (KS) is an AIDS-defining malignancy that can improve or worsen with antiretroviral therapy (ART). We aimed at identifying clinical, HIV-related, and sociodemographic factors associated with either progression or nonprogression (regression or stable disease) of ART-treated HIV-associated KS in patients with limited cutaneous disease. We conducted a prospective cohort study of ART-treated HIV-associated KS cases. Clinical, HIV-related, and sociodemographic variables were collected at baseline, and patients were followed up to determine treatment outcomes. Cox regression, linear mixed effects model, and Spearman’s rank correlation were used for analysis. Half (50%) of the study participants had KS regression or stable disease, …


Social Integration And Domestic Violence Support In An Indigenous Community: Women’S Recommendations Of Formal Versus Informal Sources Of Support, G. Robin Gauthier, Sara C. Francisco, Bilal Khan, Kirk Dombrowski Jan 2021

Social Integration And Domestic Violence Support In An Indigenous Community: Women’S Recommendations Of Formal Versus Informal Sources Of Support, G. Robin Gauthier, Sara C. Francisco, Bilal Khan, Kirk Dombrowski

Department of Sociology: Faculty Publications

Throughout North America, indigenous women experience higher rates of intimate partner violence and sexual violence than any other ethnic group, and so it is of particular importance to understand sources of support for Native American women. In this article, we use social network analysis to study the relationship between social integration and women’s access to domestic violence support by examining the recommendations they would give to another woman in need. We ask two main questions: First, are less integrated women more likely to make no recommendation at all when compared with more socially integrated women? Second, are less integrated women …


Practical Problems And Positive Experiences With Ecological Momentary Assessment: Reflections From People Who Use Drugs, Kelly Markowski, Jeffrey A. Smith, G. Robin Gauthier, Sela R. Harcey Jan 2021

Practical Problems And Positive Experiences With Ecological Momentary Assessment: Reflections From People Who Use Drugs, Kelly Markowski, Jeffrey A. Smith, G. Robin Gauthier, Sela R. Harcey

Department of Sociology: Faculty Publications

Background: Ecological momentary assessment (EMA) is an increasingly popular and feasible form of data collection, but it can be intensive and intrusive. Especially for at-risk, vulnerable populations like people who use drugs (PWUD), poor experiences with EMA may exacerbate existing chronic struggles while decreasing response rates. However, little research queries participants’ experiences with EMA studies.

Objectives: We explore participants’ positive and negative experiences with EMA, identifying what they liked about the study, the problems they experienced, and suggested solutions to these problems.

Methods: Results come from semi-structured interviews from 26 PWUD (6 women; 20 men) in Nebraska …


Constructing Pornography Addiction’S Harms In Science, News Media, And Politics, Kelsy Burke, Alice Millermacphee Jan 2021

Constructing Pornography Addiction’S Harms In Science, News Media, And Politics, Kelsy Burke, Alice Millermacphee

Department of Sociology: Faculty Publications

In order to describe pornography’s harms in the twenty-first century, an age of unprecedented access to Internet technology, some advocates—including activists, religious leaders, politicians, and scientists—use a medical/scientific framework to claim that pornography is biologically addictive. This article examines public discourse on “pornography addiction” to extend theories of sociology of science and sociology of sexualities about scientific knowledge and the biomedicalization of sex and sexuality. Using content analysis of over 600 documents, including scientific studies, newspaper articles, and state government resolutions, we show how references to pornography as addictive emerged in the twenty-first century and grew most substantially in the …


Litigating Child Sex Abuse In Domestic Relations Cases, Mike Mchenry Jan 2021

Litigating Child Sex Abuse In Domestic Relations Cases, Mike Mchenry

Court Review: Journal of the American Judges Association

IN RULING ON PARENTING TIME DISPUTES COURTS ARE SOMETIMES CALLED UPON TO MAKE FINDINGS REGARDING WHETHER A PARENT HAS ABUSED A CHILD, INCLUDING SEXUAL ABUSE.

Sometimes these abuse allegations are handled in criminal court, and if a conviction enters then the matter is considered resolved. However, if the accused parent is acquitted, or if the prosecuting authority feels the evidence is inadequate to justify a criminal prosecution, then the obligation to assess the allegation’s merits falls to the domestic relations court, where busy dockets and inexperienced practitioners can present a challenge for the judge.


Molecular Identification Of Parasites In An Intestinal Coprolite From A Mummified Religious Dignitary Of The Piraino Mother Church Crypt, Sicily, Amanda Rollins, Krystiana Krupa, Georgia Millward, Dario Piombino-Mascali, Karl Reinhard, Frederika Kaestle Jan 2021

Molecular Identification Of Parasites In An Intestinal Coprolite From A Mummified Religious Dignitary Of The Piraino Mother Church Crypt, Sicily, Amanda Rollins, Krystiana Krupa, Georgia Millward, Dario Piombino-Mascali, Karl Reinhard, Frederika Kaestle

Karl Reinhard Publications

Intestinal contents were sampled from a spontaneously enhanced mummy from the Sepulcher of the Priests of the Piraino Mother Church in the Province of Messina, Sicily. This adult male mummy, Piraino 1, is an unidentified religious dignitary dating from the late-18th to mid-19th centuries. Immunological and molecular diagnostics were used to test for common and clinically significant parasites. A morphological diagnosis of Trichuris trichiura (whipworm) was confirmed genetically. A previously undetected Enterobius vermicularis (pinworm) infection was also identified genetically. These data indicate that the Piraino 1 individual was simultaneously infected with multiple intestinal parasite species indicative of poor hygiene. This …


Differential Effects Of Whole Red Raspberry Polyphenols And Their Gut Metabolite Urolithin A On Neuroinflammation In Bv-2 Microglia, Ashley Mulcahy Toney, Mahaa Albusharif, Duncan Works, Luke Polenz, Stacie Schlange, Virginia Chaidez, Amanda E. Ramer-Tait, Soonkyu Chung Jan 2021

Differential Effects Of Whole Red Raspberry Polyphenols And Their Gut Metabolite Urolithin A On Neuroinflammation In Bv-2 Microglia, Ashley Mulcahy Toney, Mahaa Albusharif, Duncan Works, Luke Polenz, Stacie Schlange, Virginia Chaidez, Amanda E. Ramer-Tait, Soonkyu Chung

Department of Nutrition and Health Sciences: Faculty Publications

Whole red raspberry polyphenols (RRW), including ellagic acid, and their gut-derived metabolite, urolithin A (UroA), attenuate inflammation and confer health benefits. Although results from recent studies indicate that polyphenols and UroA also provide neuroprotective effects, these compounds differ in their bioavailability and may, therefore, have unique effects on limiting neuroinflammation. Accordingly, we aimed to compare the neuroprotective effects of RRW and UroA on BV-2 microglia under both 3 h and 12 and 24 h inflammatory conditions. In inflammation induced by lipopolysaccharide (LPS) and ATP stimulation after 3 h, RRW and UroA suppressed pro- inflammatory cytokine gene expression and regulated the …


Covid-19 Contagion Among Communities With Limited English Proficiency: Lesson From Volunteerism, Falah N. Rashoka M.S Jan 2021

Covid-19 Contagion Among Communities With Limited English Proficiency: Lesson From Volunteerism, Falah N. Rashoka M.S

Department of Nutrition and Health Sciences: Faculty Publications

The COVID-19 affects all communities in the United States, but its impact differs from one community to another. Undoubtedly, the vulnerable populations are more impacted than others, especially refugees and immigrants. This study seeks to understand the impact of COVID-19 on marginalized communities and the need to communicate health-related information equally. A lesson learned from observation during an ongoing pandemic, including what I learned through my volunteer work between mid-March and August 2020. The study finds that neighbors with limited English Proficiency (LEP) and limited education could quickly transfer the COVID-19 virus to another neighbor with high income and well-educated. …


The Effects Of Phosphocreatine Disodium Salts Plus Blueberry Extract Supplementation On Muscular Strength, Power, And Endurance, John Paul V. Anders, Tyler J. Neltner, Robert W. Smith, Joshua L. Keller, Terry J. Housh, F Joseph Daugherty, Michael S. Tempesta, Alekha K. Dash, Daniel J. Munt, Richard J. Schmidt, Glen O. Johnson Jan 2021

The Effects Of Phosphocreatine Disodium Salts Plus Blueberry Extract Supplementation On Muscular Strength, Power, And Endurance, John Paul V. Anders, Tyler J. Neltner, Robert W. Smith, Joshua L. Keller, Terry J. Housh, F Joseph Daugherty, Michael S. Tempesta, Alekha K. Dash, Daniel J. Munt, Richard J. Schmidt, Glen O. Johnson

Department of Nutrition and Health Sciences: Faculty Publications

Background: Numerous studies have demonstrated the efficacy of creatine supplementation for improvements in exercise performance. Few studies, however, have examined the effects of phosphocreatine supplementation on exercise performance. Furthermore, while polyphenols have antioxidant and anti-inflammatory properties, little is known regarding the influence of polyphenol supplementation on muscular strength, power, and endurance. Thus, the purpose of the present study was to compare the effects of 28 days of supplementation with phosphocreatine disodium salts plus blueberry extract (PCDSB), creatine monohydrate (CM), and placebo on measures of muscular strength, power, and endurance.

Methods: Thirty-three men were randomly assigned to consume either PCDSB, CM, …


Essential Role Of Systemic Iron Mobilization And Redistribution For Adaptive Thermogenesis Through Hif2-Α/Hepcidin Axis, Jin-Seon Yook, Mikyoung You, Jiyoung Kim, University Of Nebraska-Lincoln, Rong Fan, Bhanwar Lal Puniya, Tomáš Helikar, Sophie Vaulont, Jean-Christophe Deschemin, Meshail Oklar, Liwei Xie, Manik C. Ghosh, Tracey A. Rouault, Jaekwon Lee, Soonkyu Chung Jan 2021

Essential Role Of Systemic Iron Mobilization And Redistribution For Adaptive Thermogenesis Through Hif2-Α/Hepcidin Axis, Jin-Seon Yook, Mikyoung You, Jiyoung Kim, University Of Nebraska-Lincoln, Rong Fan, Bhanwar Lal Puniya, Tomáš Helikar, Sophie Vaulont, Jean-Christophe Deschemin, Meshail Oklar, Liwei Xie, Manik C. Ghosh, Tracey A. Rouault, Jaekwon Lee, Soonkyu Chung

Department of Nutrition and Health Sciences: Faculty Publications

Iron is an essential biometal, but is toxic if it exists in excess. Therefore, iron content is tightly regulated at cellular and systemic levels to meet metabolic demands but to avoid toxicity. We have recently reported that adaptive thermogenesis, a critical metabolic pathway to maintain whole-body energy homeostasis, is an irondemanding process for rapid biogenesis of mitochondria. However, little information is available on iron mobilization from storage sites to thermogenic fat. This study aimed to determine the iron-regulatory network that underlies beige adipogenesis. We hypothesized that thermogenic stimulus initiates the signaling interplay between adipocyte iron demands and systemic iron liberation, …


Test-Retest Reliability And Minimal Detectable Change Of The Computerized Dynamic Posturography Proprio For Adults With Chronic Traumatic Brain Injury, Guilherme Manna Cesar, Thad W. Buster, Judith M. Burnfield Jan 2021

Test-Retest Reliability And Minimal Detectable Change Of The Computerized Dynamic Posturography Proprio For Adults With Chronic Traumatic Brain Injury, Guilherme Manna Cesar, Thad W. Buster, Judith M. Burnfield

Department of Mechanical and Materials Engineering: Faculty Publications

Purpose: Balance deficits after brain injury, including reactive recovery from unexpected perturbations, can persist well after rehabilitation is concluded. While traditional clinical assessments are practical, the anticipatory nature of the tasks may mask perceptible balance control. Computerized dynamic posturography can directly quantify capacity to respond to unexpected, external perturbations. This study examined the reliability of the computerized dynamic posturography assessment with the device PROPRIO® 4000 in adults with traumatic brain injury and created the minimal detectable change for its standardized test.

Methods: Ten adults (ages 21–55 years) with chronic (average 10 ± 6 years post-injury) severe (loss of consciousness 2–75 …


Large-Scale Synthesis Of Compressible And Re-Expandable Three-Dimensional Nanofiber Matrices, Alec Mccarthy, Lorenzo Saldana, Daniel Mcgoldrick, Johnson V. John, Mitchell Kuss, Shixuan Chen, Bin Duan, Mark A. Carlson, Jingwei Xie Jan 2021

Large-Scale Synthesis Of Compressible And Re-Expandable Three-Dimensional Nanofiber Matrices, Alec Mccarthy, Lorenzo Saldana, Daniel Mcgoldrick, Johnson V. John, Mitchell Kuss, Shixuan Chen, Bin Duan, Mark A. Carlson, Jingwei Xie

Department of Mechanical and Materials Engineering: Faculty Publications

Due to their biomimetic properties, electrospun nanofibers have shown great potential in many biomedical fields. However, traditionally-produced nanofibers are typically two-dimensional (2D) membranes limiting their applications. Herein, we report a large-scale synthesis of compressible and reexpandable three-dimensional (3D) nanofiber matrices for potential biomedical applications. The reproducible mass production of such matrices is achieved using a multiple-emitter electrospinning machine with a controlled environment (e.g., temperature, humidity, and air flow rate) followed by an innovative gas-foaming expansion. The modified 20-emitter circular array with 3D-printed needle caps is capable of maintaining stable Taylor cones under extremely high flow rates. The introduction of such …


A Novel Large Animal Model Of Smoke Inhalation-Induced Acute Respiratory Distress Syndrome, Premila D. Leiphrakpam, Hannah R. Weber, Andrea Mccain, Roser Romaguera Matas, Ernesto Martinez Duarte, Keely L. Buesing Jan 2021

A Novel Large Animal Model Of Smoke Inhalation-Induced Acute Respiratory Distress Syndrome, Premila D. Leiphrakpam, Hannah R. Weber, Andrea Mccain, Roser Romaguera Matas, Ernesto Martinez Duarte, Keely L. Buesing

Department of Mechanical and Materials Engineering: Faculty Publications

Background: Acute respiratory distress syndrome (ARDS) is multifactorial and can result from sepsis, trauma, or pneumonia, amongst other primary pathologies. It is one of the major causes of death in critically ill patients with a reported mortality rate up to 45%. The present study focuses on the development of a large animal model of smoke inhalation-induced ARDS in an effort to provide the scientific community with a reliable, reproducible large animal model of isolated toxic inhalation injury-induced ARDS. Methods: Animals (n=21) were exposed to smoke under general anesthesia for 1 to 2 h (median smoke exposure=0.5 to 1 L of …


A Study On The Pathological Effects Of Trypanorhyncha Cestodes In Dusky Groupers Epinephelus Marginatus From The Canary Islands, Carolina De Sales-Ribeiro, Miguel A. Rivero, Antonio Fernández, Natalia García-Álvarez, Jorge Francisco González, Oscar Quesada-Canales, María José Caballero Jan 2021

A Study On The Pathological Effects Of Trypanorhyncha Cestodes In Dusky Groupers Epinephelus Marginatus From The Canary Islands, Carolina De Sales-Ribeiro, Miguel A. Rivero, Antonio Fernández, Natalia García-Álvarez, Jorge Francisco González, Oscar Quesada-Canales, María José Caballero

Harold W. Manter Laboratory of Parasitology: Library Materials

Trypanorhyncha are cestodes commonly infecting marine fish. Numerous studies have detailed the biology of Trypanorhyncha species, but information on the pathological changes produced by these parasites is limited. Dusky groupers are keystone species necessary for the preservation of several marine ecosystems. Considering their vulnerable state of conservation and the efforts being made to culture them, identification of the effects caused by Trypanorhyncha is vital. Here, we aimed to determine the prevalence and pathological changes produced by Trypanorhyncha in dusky groupers from the Canary Islands. The prevalence of trypanorhynch plerocerci was 96%. Grossly, in the abdominal cavity, there were numerous larvae-filled …


Predicting College Students’ Future Intentions To Engage In Public-Sphere Water Conservation Behaviors, Austin Wise, Donald Johnson, George Wardlow, Kathi Jogan Jan 2021

Predicting College Students’ Future Intentions To Engage In Public-Sphere Water Conservation Behaviors, Austin Wise, Donald Johnson, George Wardlow, Kathi Jogan

Department of Earth and Atmospheric Sciences: Faculty Publications

The purpose of this study was to describe selected college students’ (N = 252) perceptions of and future intentions (FI) to engage in public-sphere water conservation behaviors, and to determine if FI could be predicted by a single or linear combination of student demographic characteristics and latent variables. A majority of respondents agreed a growing population will negatively affect water quantity (90.5%) and there is a need for water resource management (85.6%). A majority disagreed or strongly disagreed that they (53.4%), their family (57.1%), or their friends (67.5%) practiced water conservation, or that people in their hometowns were concerned about …


Phospholipid R Ecovery From Sweet Whey And Whey Protein Concentrate: Use Of Electrodialysis With Bipolar Membrane Combined With A Dilution Factor As An Ecoefficient Method, Mélanie Faucher, Véronique Perreault, Ozan Nazim Ciftci, Sami Gaaloul, Laurent Bazinet Jan 2021

Phospholipid R Ecovery From Sweet Whey And Whey Protein Concentrate: Use Of Electrodialysis With Bipolar Membrane Combined With A Dilution Factor As An Ecoefficient Method, Mélanie Faucher, Véronique Perreault, Ozan Nazim Ciftci, Sami Gaaloul, Laurent Bazinet

Department of Food Science and Technology: Faculty Publications

Electrodialysis with bipolar membrane (EDBM) is promising to recover phospholipids (PLs) from sweet whey and whey protein concentrate (WPC), as it promotes lipoprotein complex formation following a decrease in pH and ionic strength. The aim of this work was to study the impact of dilution factor (without dilution, with a 2X, 4X and 6X dilution) after EDBM on the process performances. For both products, a 4X dilution, which cor- responds to a decrease in ionic strength of 81.4 ± 1.5 % for sweet whey and 79.4 ± 0.4 % for WPC, seemed sufficient to maximize lipoprotein complex formation as a …


Mapping Collaborations And Partnerships In Sdg Research, Jane Payumo, Guangming He, Anusha Chintamani Manjunatha, Devin Higgins, Scout Calvert Jan 2021

Mapping Collaborations And Partnerships In Sdg Research, Jane Payumo, Guangming He, Anusha Chintamani Manjunatha, Devin Higgins, Scout Calvert

University of Nebraska-Lincoln Libraries: Faculty Publications

Collaboration has become an essential paradigmin sustainable development research and in strategies for meeting the United Nations Sustainable Development Goals (SDGs). This study uses bibliometric methods and network analysis to examine research output and collaboration supporting the SDGs and explores means to detect and analyze research collaboration beyond the traditional definition of multiple, one-time co-authorship. We employed two additional lenses of collaboration: repeat collaboration and collaboration time point to quantify and visualize co-authorship data sourced from Microsoft Academic Graph. Our results show an increased collaboration rate over time at the author and institutional levels; however they also indicate that the …


A Hybrid Biological-Adsorption Approach For The Treatment Of Contaminated Groundwater Using Immobilized Nanoclay-Algae Mixtures, Sara Mollamohammada, Ashraf Aly Hassan, Mohamed Dahab, Sandeep Kumar Jan 2021

A Hybrid Biological-Adsorption Approach For The Treatment Of Contaminated Groundwater Using Immobilized Nanoclay-Algae Mixtures, Sara Mollamohammada, Ashraf Aly Hassan, Mohamed Dahab, Sandeep Kumar

Department of Civil and Environmental Engineering: Faculty Publications

Mixing the Scenedesmus species with nanoclay and immobilizing in sodium was evaluated as a sustainable treatment method for removing nitrate, atrazine, and metals from groundwater. Gel beads containing the hybrid mixture removed 100% of 10 mg/L N nitrate and 98% of 100 μg/L atrazine from synthetic groundwater in three days. The optimal amount of nanoclay was found to be 0.30 mg per bead. The experimental data fit well into a Freundlich adsorption iso- therm and followed pseudo first-order kinetics. When tested in actual groundwater, 91% of nitrate and 100% of Cr, Se, and V were eliminated in three days without …