Digital Commons Network^™

Considering The Needs, Challenges, And Assets Of School Systems In Rural Meatpacking Communities During Covid-19: The Roles Of Schools As Vehicles Of Community, Edmund T. Hamann, Amanda R. Morales, Ricardo Martinez

Department of Teaching, Learning, and Teacher Education: Faculty Publications

For more than 40 years, the relocation of meatpacking from urban to rural communities and the related change of who works in these high-risk, modestly compensated positions has brought Spanish-speaking and African and Asian refugee populations to communities like the seven focal Nebraska communities we consider here. These seven, five rural and two part of multi-county metropolitan areas, all have majority Latinx school enrollments and they all have been particularly hard hit by both the first and third waves of the COVID-19 pandemic. They were each also foci of a special new course we co-taught in December 2020 called: “Pandemics, …

Barriers To Summer Food Service Program (Sfsp) Implementation Before And After Covid-19: A Qualitative, Collective Case Study, Masoomeh Hajizadeh Oghaz, Falah N. Rashoka, Megan S. Kelley

Department of Nutrition and Health Sciences: Faculty Publications

Objectives: SFSP, as a federal food assistance program, could ensure that children have sufficient nutritious food during summer. According to a 2016 USDA report, only 0.4–0.6% of eligible children participated in this program in Nebraska, which makes for one of the lowest SFSP participation rates in the U.S. This study aims to better understand how the COVID-19 pandemic has impacted SFSP implementation in urban and rural settings across the state.

Conclusions: Insights from this study may inform the development of policies that expand food access to vulnerable families. On the organizational level, this includes providing participating kids and their parents …

“Many People Have No Idea”: A Qualitative Analysis Of Healthcare Barriers Among Yazidi Refugees In The Midwestern United States, Falah N. Rashoka, Megan S. Kelley, Jeong‑Kyun Choi, Marc A. Garcia, Weiwen Chai, Hazim N. Rashawka

Department of Nutrition and Health Sciences: Faculty Publications

Background: The COVID-19 pandemic has shed new light on inequities in healthcare access faced by immigrant and refugee communities. To address ongoing disparities, there is an urgent need for ecological approaches to better understand the barriers that hinder and resources that facilitate access to healthcare. This study investigates barriers to healthcare system access faced by Yazidi refugees in the Midwestern United States.

Methods: Informed by the Interpretative Phenomenological Approach, three focus group meetings with a community advisory board were conducted between September 2019 and January 2020. The nine-member focus group included social workers, healthcare providers, and members of the Yazidi …

The Impact Of Covid-19 On Perceived Barriers And Facilitators To The Healthfulness Of Communities With Low-Income, Elder Varela, Jamie Zeldman, Alison Kuch, Yanli Wang, Mohammad Shushari, Elizabeth L. Hall, Jade Mcnamara, Jesse Stabile Morrell, Terezie Tolar-Peterson, Onikia Brown, Melissa D. Olfert, Lisa Franzen-Castle, Tandalayo Kidd, Sarah Colby, Geoffrey Greene, Karla P. Shelnutt

Department of Nutrition and Health Sciences: Faculty Publications

Background: The COVID-19 pandemic brought new challenges affecting the wellbeing of individuals in communities with low income. Understanding where people live and how those environments can facilitate or hinder living a healthy lifestyle is essential for developing interventions that target behavior change and health promotion. Objective: This study compares Extension Nutrition Educators’ (NEs) perceptions of the barriers and facilitators impacting the healthfulness of the environment of communities with low income in eleven states before and during the COVID-19 pandemic.

High Expression Of Glycolytic Genes In Clinical Glioblastoma Patients Correlates With Lower Survival, Kimberly M. Stanke, Carrick Wilson, Srivatsan Kidambi

Department of Chemical and Biomolecular Engineering: Faculty Publications

Glioblastoma (GBM), the most aggressive brain tumor, is associated with a median survival at diagnosis of 16–20 months and limited treatment options. The key hallmark of GBM is altered tumor metabolism and marked increase in the rate of glycolysis. Aerobic glycolysis along with elevated glucose consumption and lactate production supports rapid cell proliferation and GBM growth. In this study, we examined the gene expression profile of metabolic targets in GBM samples from patients with lower grade glioma (LGG) and GBM. We found that gene expression of glycolytic enzymes is up-regulated in GBM samples and significantly associated with an elevated risk …

Evaluation Of Salmonella Biofilm Cell Transfer From Common Food Contact Surfaces To Beef Products, Rong Wong, David A. King, Norasak Kalchayanand

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Meat contamination by Salmonella enterica is a serious public health concern. Available data have suggested that biofilm formation at processing plants and contaminated contact surfaces might contribute to meat contamination. Because transfer from contact surfaces to food products via direct contact has been deemed the most common bacteria transmission route that can lead to contamination, we evaluated the effect of Salmonella biofilm forming ability, contact surface material, and beef surface tissue type on Salmonella biofilm transfer from hard surfaces to beef products. Salmonella biofilms developed on the common contact surfaces stainless steel (SS) and polyvinylchloride (PVC) were transferred consecutively via …

Caregiver Participation Engagement In Child Mental Health Prevention Programs: A Systematic Review, Rachel Haine‑Schlagel, Kelsey S. Dickson, Teresa Lind, Joanna J. Kim, Gina C. May, Natalia Escobar Walsh, Vanja Lazarevic, Brent R. Crandal, May Yeh

Department of Psychology: Faculty Publications

Prevention programs are a key method to reduce the prevalence and impact of mental health disorders in childhood and adolescence. Caregiver participation engagement (CPE), which includes caregiver participation in sessions as well as follow-through with homework plans, is theorized to be an important component in the effectiveness of these programs. This systematic review aims to (1) describe the terms used to operationalize CPE and the measurement of CPE in prevention programs, (2) identify factors associated with CPE, (3) examine associations between CPE and outcomes, and (4) explore the effects of strategies used to enhance CPE. Thirty-nine articles representing 27 unique …

Sustainability Around The World, Haley Withuhn, Ethan Halman Gonzalez

Honors Program: Expanded Learning Clubs

No abstract provided.

Workplace Incivility, Megan Paul

Umbrella Summaries

What is workplace incivility?

Workplace incivility refers to “low-intensity deviant behavior with ambiguous intent to harm the target, in violation of workplace norms for mutual respect. Uncivil behaviors are characteristically rude and discourteous, displaying a lack of regard for others” (Anderson & Pearson, 1999, p. 457). Though there is some conceptual and empirical overlap between incivility and ostracism, bullying, and abusive supervision, incivility is considered distinct (Yao et al., 2021). Incivility has been examined from the perspective of both the victim and the instigator, exploring factors related to being the target of incivility and factors related to engaging in uncivil …

Genetic Diversity Of Huaya India (Melicoccus Oliviformis Kunth), A Neglected Neotropical Fruit Crop, Mónica I. Jiménez-Rojas, Rubén H. Andueza-Noh, Obed I. Noh-Ake, Daniel Potter, Matilde M. Ortiz-García, Renee S. Arias, Jaime Martínez-Castillo

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Currently, some species of Sapindaceae are important fruit crops worldwide. The Huaya India (Meliccocus oliviformis, Sapindaceae) is a neglected Neotropical fruit tree consumed locally in the Maya Lowlands of Mexico, where it exists in both wild and domesticated forms. Our objective was to evaluate the genetic diversity of the Huaya India in its possible domestication area and thus generate knowledge that serves as the basis for a commercial management. A total of 450 individuals collected from 15 natural vegetation sites and 15 Maya villages, were characterized using nine microsatellite loci and population genetics approaches were applied. STRUCTURE, Neighbor-Joining and PCoA …

Low-Level Groundwater Atrazine In High Atrazine Usage Nebraska Counties: Likely Effects Of Excessive Groundwater Abstraction, Moses New-Aaron, Olufemi P. Abimbola, Raheleh Mohammadi, Oluwaseun Famojuro, Zaeema Naveed, Azar M Abadi, Jesse E. Bell, Shannon Bartelt-Hunt, Eleanor G. Rogan

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Recent studies observed a correlation between estrogen-related cancers and groundwater atrazine in eastern Nebraska counties. However, the mechanisms of human exposure to atrazine are unclear because low groundwater atrazine concentration was observed in counties with high cancer incidence despite having the highest atrazine usage. We studied groundwater atrazine fate in high atrazine usage Nebraska counties. Data were collected from Quality Assessed Agrichemical Contaminant Nebraska Groundwater, Parameter–Elevation Regressions on Independent Slopes Model (PRISM), and water use databases. Descriptive statistics and cluster analysis were performed. Domestic wells (59%) were the predominant well type. Groundwater atrazine was affected by well depth. Clusters consisting …

Comparison Of Methods To Estimate Ruminal Degradation And Intestinal Digestibility Of Protein In Hydrolyzed Feather Meal With Or Without Blood, Kassidy Buse, D. L. Morris, H. L. Diaz, O. R. Drehmel, Paul J. Kononoff

Department of Animal Science: Faculty Publications

Hydrolyzed feather meal (HFM) is a feedstuff high in rumen undegraded protein (RUP) that can be used as an effective source of metabolizable protein for dairy cattle. Because the production process may vary, the rumen degradability and intestinal digestibility of HFM may also vary. Additionally, some processes may incorporate additional blood into the final product to result in feather meal with poultry blood. To determine the rumen degradability and intestinal digestibility of these products, several laboratory assays can be used; the common assays are the mobile bag (MOB), modified three-step (MTS), and Ross (ROS) assays. Although all 3 assays determine …

Electrochemical Beacon Method To Quantify 10 Attomolar Nucleic Acids With A Semilog Dynamic Range Of 7 Orders Of Magnitude, Rahul Tevatia, Alicia Chan, Lance Oltmanns, Jau Min Lim, Ander Christensen, Michael Stroller, Ravi Saraf

Department of Chemical and Biomolecular Engineering: Faculty Publications

Change in the dynamics of single-stranded DNA or RNA probes tethered to an Au electrode on immunospecific binding to the analyte is a versatile approach to quantify a variety of molecules, such as heavy metal ions, pesticides, proteins, and nucleic acids (NAs). A widely studied approach is the electrochemical beacon method where the redox of a dye attached to the probe decreases as its proximity to the underlying electrode changes on binding. The limit of quantification (LOQ) defined by the semilog dependence of the signal on target concentration is in the picomolar range. Here, a method was studied where, by …

A Link Between Methylglyoxal And Heart Failure During Hiv-1 Infection, Prasanta K. Dasg, Fadhel A. Alomar, Jesse L. Cox, Joellyn Mcmillan, Bryan T. Hackfort, Edward Makarov, Brenda Morsey, Howard S. Fox, Howard E. Gendelman, Santhi Gorantla, Keshore R. Bidasee

Department of Biochemistry: Faculty Publications

Early-onset heart failure (HF) continues to be a major cause of morbidity and mortality in people living with human immunodeficiency virus type one (HIV-1) infection (PLWH), yet the molecular causes for this remain poorly understood. Herein NOD.Cg- Prkdc^scidIl2rg^tm1Wjl/SzJ humanized mice (Hu-mice), plasma from PLWH, and autopsied cardiac tissues from deceased HIV seropositive individuals were used to assess if there is a link between the glycolysis byproduct methylglyoxal (MG) and HF in the setting of HIV-1 infection. At five weeks post HIV infection, Hu-mice developed grade III-IV diastolic dysfunction (DD) with an associated two-fold increase in plasma …

Inflammatory Mediation Of Heat Stress-Induced Growth Deficits In Livestock And Its Potential Role As A Target For Nutritional Interventions: A Review, Micah S. Most, Dustin T. Yates

Department of Animal Science: Faculty Publications

Heat stress is detrimental to well-being and growth performance in livestock, and systemic inflammation arising during chronic heat stress contributes to these poor outcomes. Sustained exposure of muscle and other tissues to inflammation can impair the cellular processes that facilitate muscle growth and intramuscular fat deposition, thus reducing carcass quality and yield. Climate change is expected to produce more frequent extreme heat events, increasing the potential impact of heat stress on sustainable livestock production. Feedlot animals are at particularly high risk for heat stress, as confinement limits their ability to seek cooling from the shade, water, or breeze. Economically practical …

I Am A Cat, Natsume Sōseki

Zea E-Books Collection

This English version of 吾輩は猫である (Wagahai-wa neko de aru: I Am a Cat), Chapters I and II, written by Natsume Sōseki, pseudonym of Natsume Kinnosuke (1867–1916), and translated by Kan-ichi Ando (1878-1924), was published by Hattori Shoten, Tokyo, in 1906.

It begins: "I am a cat; but as yet I have no name." Its sardonic feline narrator describes his origins, his settlement in the household of a Meiji teacher-intellectual, and the goings-on and conversations among the cats and humans about the neighborhood. Of the men he concludes: "They are miserable creatures in the eyes of a cat."

Japanese novelist Natsume …

A Review Of Transformative Strategies For Climate Mitigation By Grasslands, Nuria Gomez-Casanovas, Elena Blanc-Betes, Caitlin E. Moore, Carl J. Bernacchi, Ilsa Kantola, Evan H. Delucia

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Grasslands can significantly contribute to climate mitigation. However, recent trends indicate that human activities have switched their net cooling effect to a warming effect due to management intensification and land conversion. This indicates an urgent need for strategies directed to mitigate climate warming while enhancing productivity and efficiency in the use of land and natural (nutrients, water) resources. Here, we examine the potential of four innovative strategies to slow climate change including: 1) Adaptive multi-paddock grazing that consists of mimicking how ancestral herds roamed the Earth; 2) Agrivoltaics that consists of simultaneously producing food and energy from solar panels on …

Audiovisual Metadata Platform Pilot Development (Amppd), Final Project Report, Jon W. Dunn, Ying Feng, Juliet L. Hardesty, Brian Wheeler, Maria Whitaker, Thomas Whittaker, Shawn Averkamp, Bertram Lyons, Amy Rudersdorf, Tanya Clement, Liz Fischer

Copyright, Fair Use, Scholarly Communication, etc.

This report documents the experience and findings of the Audiovisual Metadata Platform Pilot Development (AMPPD) project, which has worked to enable more efficient generation of metadata to support discovery and use of digitized and born-digital audio and moving image collections. The AMPPD project was carried out by partners Indiana University Libraries, AVP, University of Texas at Austin, and New York Public Library between 2018-2021.

Ten Simple Rules For Creating A Scientific Web Application, Jessica L. Burnett, Renee Dale, Chung-Yi Hou, Gabriela Palomo-Muoz, Kaitlin Stack Whitney, Steve Aulenbach, Robert Sky Bristol, Denis Valle, Tristan P. Wellman

School of Natural Resources: Faculty Publications

The use of scientific web applications (SWApps) across biological and environmental sciences has grown exponentially over the past decades or so. Although quantitative evidence for such increased use in practice is scant, collectively, we have observed that these tools become more commonplace in teaching, outreach, and in science coproduction (e.g., as decision support tools). Despite the increased popularity of SWApps, researchers often receive little or no training in creating such tools. Although rolling out SWApps can be a relatively simple and quick process using modern, popular platforms like R shiny apps or Tableau dashboards, making them useful, usable, and sustainable …

Source Apportionment And Health Risk Assessment Of Nitrate In Foothill Aquifers Of Western Ghats, South India, Banajarani Panda, S. Chidambaram, Daniel D. Snow, Arindam Malakar, Dhiraj Kr Singh, L. Ramanathan

Nebraska Water Center: Faculty Publications

The present research reports the level of nitrate (NO₃^-), associated health risks and possible sources of contamination in groundwater from south India. Many samples (32%) are above or approaching the recommended level of NO₃^- for safe drinking water. The correlation analysis indicates different sources of NO₃^- contamination in different regions rather than a common origin. The isotopic measurements provide information about potential nitrogen sources contributing NO₃^- to the groundwater. Based on isotope analysis, the sources of NO₃^- in the groundwater of this region are likely to be from (a) …

The Price Of Surviving On Adrenaline: Developmental Programming Responses To Chronic Fetal Hypercatecholaminemia Contribute To Poor Muscle Growth Capacity And Metabolic Dysfunction In Iugr-Born Offspring, Rachel L. Gibbs, Dustin T. Yates

Department of Animal Science: Faculty Publications

Maternofetal stress induces fetal programming that restricts skeletal muscle growth capacity and metabolic function, resulting in intrauterine growth restriction (IUGR) of the fetus. This thrifty phenotype aids fetal survival but also yields reduced muscle mass and metabolic dysfunction after birth. Consequently, IUGR-born individuals are at greater lifelong risk for metabolic disorders that reduce quality of life. In livestock, IUGR-born animals exhibit poor growth efficiency and body composition, making these animals more costly and less valuable. Specifically, IUGR-associated programming causes a greater propensity for fat deposition and a reduced capacity for muscle accretion. This, combined with metabolic inefficiency, means that these …

Smooth Muscle Cells Affect Differential Nanoparticle Accumulation In Disturbed Blood Flow-Induced Murine Atherosclerosis, Hunter Miller, Morgan A. Schake, Badrul Alam Bony, Evan T. Curtis, Conner C. Gee, Ian Mccue, Thomas J. Ripperda Jr., Yiannis S. Chatzizisis, Forrest Kievit, Ryan M. Pedrigi

Department of Mechanical and Materials Engineering: Faculty Publications

Atherosclerosis is a lipid-driven chronic inflammatory disease that leads to the formation of plaques in the inner lining of arteries. Plaques form over a range of phenotypes, the most severe of which is vulnerable to rupture and causes most of the clinically significant events. In this study, we evaluated the efficacy of nanoparticles (NPs) to differentiate between two plaque phenotypes based on accumulation kinetics in a mouse model of atherosclerosis. This model uses a perivascular cuff to induce two regions of disturbed wall shear stress (WSS) on the inner lining of the instrumented artery, low (upstream) and multidirectional (downstream), which, …

Injectable Decellularized Nucleus Pulposus Tissue Exhibits Neuroinhibitory Properties, Logan M. Piening, David J. Lillyman, Fei San Lee, Alvaro Moreno Lozano, Jeremy R. Miles, Rebecca A. Wachs

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background: Chronic low back pain (LBP) is a leading cause of disability, but treatments for LBP are limited. Degeneration of the intervertebral disc due to loss of neuroinhibitory sulfated glycosaminoglycans (sGAGs) allows nerves from dorsal root ganglia to grow into the core of the disc. Treatment with a decellularized tissue hydrogel that contains sGAGs may inhibit nerve growth and prevent discassociated LBP.

Methods: A protocol to decellularize porcine nucleus pulposus (NP) was adapted from previous methods. DNA, sGAG, α-gal antigen, and collagen content were analyzed before and after decellularization. The decellularized tissue was then enzymatically modified to be …

Evidence Of Western Corn Rootworm (Diabrotica Virgifera Virgifera Leconte) Field-Evolved Resistance To Cry3bb1 + Cry34/ 35ab1 Maize In Nebraska, Jordan D. Reinders, Emily E. Reinders, Emily Robinson, Bryan W. French, Lance J. Meinke

Department of Entomology: Faculty Publications

BACKGROUND: Western corn rootworm (WCR; Diabrotica virgifera virgifera) field-evolved resistance to transgenic maize expressing the Cry3Bb1 protein derived from Bacillus thuringiensis (Bt) has been confirmed across the United States Corn Belt. Although use of pyramided hybrids expressing Cry3Bb1 + Cry34/35Ab1 has increased in recent years to mitigate existing WCR Bt resistance, susceptibility of Nebraska WCR populations to this rootworm–Bt pyramid has not been assessed. Plant-based bioassays were used to characterize the susceptibility of WCR populations to Cry3Bb1 and Cry3Bb1 + Cry34/35Ab1 maize. Populations were collected from areas of northeastern Nebraska with a history of planting Bt maize that expressed …

Irrigation Systems Management, Dean Eisenhauer, Derrel L. Martin, Derek M. Heeren, Glenn J. Hoffman

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Management of irrigation systems should be based on the desired objectives or outcomes consistent with economic, energy, environmental, labor, water, and resource constraints. Goals can vary from maximizing profit, producing a contracted yield, optimizing water resource use, maintaining the quality of produce, or assuring an attractive landscape. Managers cannot achieve these goals without considering the performance of the irrigation system. This chapter discusses the basic characteristics of various irrigation systems, defines terms that quantify performance, describes basic requirements all systems must provide, gives a range of attributes for systems, and discusses how water supply requirements are governed by ET and …

Mechanical Overload-Induced Muscle-Derived Extracellular Vesicles Promote Adipose Tissue Lipolysis, Ivan J. Vechetti Jr, Bailey D. Peck, Yuan Wen, R Grace Walton, Taylor R. Valentino, Alexander P. Alimov, Cory M. Dungan, Douglas W. Van Pelt, Ferdinand Von Walden, Bjorn Alkner, Charlotte A. Peterson, John J. Mccarthy

Department of Nutrition and Health Sciences: Faculty Publications

How regular physical activity is able to improve health remains poorly understood. The release of factors from skeletal muscle following exercise has been proposed as a possible mechanism mediating such systemic benefits. We describe a mechanism wherein skeletal muscle, in response to a hypertrophic stimulus induced by mechanical overload (MOV), released extracellular vesicles (EVs) containing muscle-specific miR-1 that were preferentially taken up by epidydimal white adipose tissue (eWAT). In eWAT, miR-1 promoted adrenergic signaling and lipolysis by targeting Tfap2α, a known repressor of Adrβ3 expression. Inhibiting EV release prevented the MOV-induced increase in eWAT miR-1 abundance and expression of …

Novel Approaches For Enhancing Cell Survival And Function In Vivo, Ou Wang

Department of Chemical and Biomolecular Engineering: Dissertations, Theses, and Student Research

FDA has approved several cell-based therapeutics and hundreds of cell therapy clinical trials are ongoing. Cells will be a significant type of medicine after small molecule and protein drugs. However, several obstacles need to be addressed to achieve the widespread use of cellular therapeutics. The first challenge is the low efficacy of cell transplantation due to low retention, survival, integration, and function of cells in vivo. The second challenge is producing a massive number of cells for clinical treatment with cost-effectively and reproducibly technologies.

In this thesis, we proposed and investigated two approaches to address these challenges. To begin …

Validation Of A Brazilian Portuguese Measure Of Family Resources In A Sample Of Parents To Children With Congenital Zika Virus Syndrome, Sarah Barker Ladd

College of Education and Human Sciences: Dissertations, Theses, and Student Research

In the aftermath of the 2015 Zika virus outbreak in Brazil, thousands of children and their families continue to face challenges associated with Congenital Zika Syndrome, a developmental condition associated with microcephaly and other serious birth defects affecting the central nervous system. Family-centered rehabilitative care is critical to optimize the trajectories of children who have significant developmental disabilities like CZS. These services involve assessing for the presence of family resources that may help to promote positive outcomes for children. Little is known regarding family resources in the context of caring for a child with CZS in Brazil due to the …

The Molecular Basis For Natural Competence In Acinetobacter, Yafan Yu

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Bacteria use Horizontal Gene Transfer (HGT) to acquire genetic material, leading to the development of novel traits, such as the spread and development of antibiotic resistance. Natural competence is one type of HGT accomplished by DNA uptake from the environment and incorporation into the genome. Bacteria from Acinetobacter are wildly distributed in the environment and are naturally competent. This propensity is a key factor to the steady increase in drug resistance in Acinetobacter, which is a cause for concern in human health as Acinetobacter is a major source of nosocomial infections. During natural competence, type IV pili (T4P) and related …

The Application Of Mathematical Optimization And Flavor-Detection Technologies For Modeling Aroma Of Hops, Yutong Liu

Department of Food Science and Technology: Dissertations, Theses, and Student Research

In recent years, proprietary hops (Citra, Simcoe, and Mosaic) become the most sought-after hops among brewers due to their excellent aroma. However, they are restricted to the owners unless other growers purchase the costly licensing agreements. Many public hops are available to the growers without any additional costs, but their aroma is difficult to match to the proprietary hops. Although proprietary and public hop varieties are unique in their aroma profiles, all hops varieties contain similar volatile compounds, merely differ in the quantity of different individual compounds. The main objective of this thesis was to investigate the feasibility of matching …