Digital Commons Network^™

Evaluation Of The Feeding Value Of Proso Millet In Growing-Finishing Diets For Pigs And Effects Of Feed Ingredients And Medium-Chain Fatty Acids On Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Survivability, Khang Nguyen

Department of Animal Science: Dissertations, Theses, and Student Research

The objective of the project were to: 1) evaluate the feeding value of proso millet as a potential substitute for corn in corn-soybean meal-based diets for pigs; and 2) investigate the relationship between feed ingredients and virus survivability, followed by examining feed additive candidates that could mitigate the risk of transmission of viral pathogens.

In Exp. 1, 36 barrows were fed 1 of 4 dietary treatments, including: Diet 1, corn-soybean meal-based (control), and Diets 2, 3, and 4 had proso millet replacing 33%, 67%, and 100% of corn in the control diet, respectively. During Phase 3, pigs consuming the proso …

Impact Of Increasing Level Of Milk Production On Cow And Calf Behavior And Performance In The Nebraska Sandhills, Selby L. Boerman

Department of Animal Science: Dissertations, Theses, and Student Research

Selection for increased milk production across the United States has resulted in variable cow and calf production responses. Better understanding of calf nursing and grazing behaviors may provide opportunities to help estimate how milk and grazed forage intake influence variability in calf performance. In a 2-yr study, cow-calf pairs (n = 65) were equipped with neck collars containing global positioning system (GPS) units to validate the efficacy of high-frequency GPS data to accurately identify calf nursing events and grazing behavior. Data were collected during 3-wk tracking periods during early lactation (calf age 65.4 ± 10.0 d; EARLY) and late …

Investigating Bacterial Community Composition And Antimicrobial Resistance Genes In Beef Cattle, Arena E. See

Department of Animal Science: Dissertations, Theses, and Student Research

While antibiotics have been critical over the last decade for curbing infectious diseases, they have contributed to the rise of antimicrobial resistance (AMR). Infections resulting from antimicrobial-resistant pathogens are correlated with severe morbidity, mortality, and healthcare costs. With the increasing concerns regarding AMR and its emergence, it is paramount to understand not only the effects of antibiotics on microbial community and diversity, but also to develop a novel tool that gives us a better understanding of the spread and functional traits of AMR genes.

To this end, rumen, fecal, and pen surface samples were collected from 72 animals at United …

Implications Of Ancestry Estimation: An Analysis Of Identification Rates In Unidentified Persons Cases, Gabrielle Mace

Department of Anthropology: Theses and Student Research

Recently, attention has been drawn to the biases present in the methodologies employed by Forensic Anthropologists, and in the medicolegal system, towards People of Color throughout the identification process. As one of the important contributors to the medicolegal system, it is essential that forensic anthropologists understand the impact of their analyses on the identification rate of marginalized unidentified decedents. Thus, through the utilization of positive identification records from Wayne and Ingham Counties in Michigan, U.S., this research investigated the disparities in identification rates between decedents reported as White and those reported as People of Color (POC). The data indicated that …

Non-Nutritive Suck Burst Pattern Stability In Extremely Premature Infants, Alejandra Marquez

College of Education and Human Sciences: Dissertations, Theses, and Student Research

The development of non-nutritive suck (NNS) burst dynamics in preterm infants reflects the integrity of the brain and is used clinically to assess feeding readiness and orofacial motor development (Mizuno and Ueda, 2005). The application of NNS analytics in the present report represents one outcome measurement set that is part of an ongoing clinical trial involving extremely preterm infants (EPI’s,[GA]) randomized to receive either pulsed orocutaneous stimulation therapeutics or a sham (blind pacifier), in conjunction with salivary sampling twice weekly to map gene expression of key proteins involved in neural development and molecular sensing of feeding related pathways in the …

Whiteflies (Hemiptera: Aleyrodidae) Intercepted On Plant Product Imported To South Korea From 2013–2021, Suhyeon Lee, Soo-Jung Suh

Insecta Mundi

Current globalization and technological progress has facilitated and increased the international trade of plant products worldwide and has promoted the long-distance movement of immobile sucking pests such as whiteflies attached on plants. Therefore, being able to compile and update information on intercepted insect pests will help to improve the inspection procedures, to detect, identify and mitigate the damage caused by exotic invasive pests. Records of whiteflies (Hemiptera: Aleyrodidae) intercepted on import plants from 2013 to 2021 in the Pest Information System (PIS) database of South Korea were analyzed. A total of 32 species belonging to 19 genera were intercepted on …

Natural History Of Cassida Sphaerula Boheman, 1854 (Coleoptera: Chrysomelidae: Cassidinae: Cassidini) On Arctotheca Prostrata (Salisb.) Britten (Asteraceae: Arctotidinae) In South Africa, With A Checklist Of South African Cassidinae (Leaf-Mining And Tortoise Beetles), Sally Adam, Mariana Campos, Hugh D.C. Heron, C. L. Staines, Rob Westerduijn, Caroline Simmrita Chaboo

Insecta Mundi

The tortoise beetle, Cassida sphaerula Boheman, 1854 (Coleoptera: Chrysomelidae: Cassidinae: Cassidini) is endemic to South Africa. Its endemic host, Arctotheca prostrata (Salisb.) Britten (Asteraceae) has been introduced in other countries where it is becoming invasive. Cassida sphaerula could provide a potential biocontrol of Arctotheca weeds as it spends the entire life cycle on this host. An intensive field study, with rearing, photography, and short films of C. sphaerula was conducted in its native habitat to document the life cycle. A checklist of Cassidinae genera in South Africa, along with 19 new host records for Cassidini species in South Africa are …

Quantification And Machine Learning Based N2o–N And Co2–C Emissions Predictions From A Decomposing Rye Cover Crop, Deepak R. Joshi, David E. Clay, Sharon A. Clay, Janet Moriles-Miller, Aaron L. M. Daigh, Graig Reicks, Shaina Westhoff

Department of Agronomy and Horticulture: Faculty Publications

Cover crops improve soil health and reduce the risk of soil erosion. However, their impact on the carbon dioxide equivalence (CO_2e) is unknown. Therefore, the objective of this 2-yr study was to quantify the effect of cover crop-induced differences in soil moisture, temperature, organic C, and microorganisms on CO_2e, and to develop machine learning algorithms that predict daily N₂O–N and CO₂–C emissions. The prediction models tested were multiple linear regression, partial least square regression, support vector machine, random forest (RF), and artificial neural network. Models’ performance was accessed using R², …

Studies On Air Injection In A Wastewater-Fed Subsurface Drip Irrigation System, Henry A. Gonzalez Hernandez

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

In 2020, the Ogallala High Plains Aquifer supplied irrigation water for approximately 3.6 million hectares in Nebraska, making it the most irrigated state in the United States. Although Nebraska is highly dependent in groundwater, locations included in the Gering-Fort Laramie Irrigation District (border of Nebraska and Wyoming) rely on surface water resources. In addition to these conventional water sources, using non-conventional alternatives for crop production can be just as important during water shortage times such as the one that e occurred in 2019 with the collapse of the Goshen/Gering-Fort Laramie Canal. Feedlot runoff presents a great opportunity in Nebraska, but …

Development Of A Decellularized Hydrogel Composite And Its Application In A Novel Model Of Disc-Associated Low Back Pain In Female Sprague Dawley Rats, David Lillyman

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Chronic low back pain is a global socioeconomic crisis compounded by an absence of reliable, curative treatments. The predominant pathology associated with chronic low back pain is degeneration of intervertebral discs in the lumbar spine. During degeneration, nerves can sprout into the intervertebral disc tissue and be chronically subjected to inflammatory and mechanical stimuli, resulting in pain. Pain arising from the intervertebral disc, or disc-associated pain, is a complex, multi-faceted disorder which necessitates valid animal models to screen therapeutics and study pathomechanisms of pain.

While many research teams have created animal models of disc degeneration, the translation of these platforms …

Efficiency Of Bank Filtration In The Removal Of Cyanotoxins Under Different Levels Of Nutrients, Luke Walkenhorst

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Harmful Algal Blooms (HABs) are an increasing concern not only in Nebraska, but across the world. Increasing global temperatures in conjunction with overuse of synthetic fertilizers are leading causes to the increasing frequency and concern of HABs. In the present work, the effectiveness of simulated bank filtration (BF), as an economical water treatment option, to remove cyanotoxins under different levels of nutrients (nitrates and phosphates) was investigated. Vertical flow-through columns pre-acclimated with two levels of nutrients (e.g., nitrate and phosphate—10 vs. 50 mg/L) were exposed to two different levels of cyanotoxins (10 vs. 75 μg/L). Results from the study confirmed …

Coupling Dendrochronology And Remote Sensing Techniques To Assess The Biophysical Traits Of Juniperus Virginiana And Pinus Ponderosa Within Grassland Communities In The Semi-Arid Grasslands Of The Nebraska Sandhills, Reece Allen

School of Natural Resources: Dissertations, Theses, and Student Research

Woody species encroachment is occurring within the sandhills region in Nebraska, primarily driven by Juniperus virginiana and Pinus ponderosa, altering ecosystems and the services they provide. Effective, low cost, and cross-scale monitoring of woody species growth and performance is necessary for integrated grassland and forest management in the face of climate variability and change. In this study, we sought to establish a relationship between remote sensing-derived vegetation indices (VIs) and dendrochronological (raw and standardized tree ring width) measurements to assess the performance of encroaching woody J. virginiana and P. ponderosa located within the Nebraska National Forest in the sandhills. …

Simulating Sub-Threshold Communication Channels Through Neurons, Richard Maina

School of Computing: Dissertations, Theses, and Student Research

Molecular Communication is an emerging paradigm with the potential to revolutionize the technology behind wearable and implantable devices and the broad range of functions they support, from tracking physical activity to medical diagnostics. This can be achieved through intra-body communication networks that take advantage of natural biological processes as a means of transmitting, propagating and receiving information. In this thesis we focus particularly on using the neuron as a means to facilitate information transfer for interconnected wearable or implantable devices through a technique known as sub-threshold electrical stimulation. We develop upon a prior work by introducing a linear model of …

Understanding The Role Of Antioxidant Nanoparticles In Improving The Outcome Of Secondary Injury In Traumatic Brain Injury, Aria W. Tarudji

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Following traumatic brain injury (TBI), excess reactive oxygen species (ROS) and other free radicals are released, inducing the cascade of secondary injury that exacerbate the outcomes of TBI. Antioxidant nanoparticles (ANPs) have shown promising outcomes in reducing the progression of TBI, which may be due to the higher accumulation and retention of ANPs in the injured brain. However, there is limited knowledge of: 1) antioxidant activities needed in TBI treatment, 2) correlation between longer retention, bioavailability, and target engagement with antioxidant treatments, and 3) sexual dimorphism to ANP treatments.

This dissertation assesses multiple ANPs with various scavenging activities and durations …

Role Stress, Sarah Stepanek, Megan Paul

Umbrella Summaries

What is role stress?

A work role consists of “a pattern of behaviors perceived by an employee as behaviors that are expected” (Tubre & Collins, 2000, p. 156). When perceived work role expectations are unclear, incompatible with other expected behaviors, or too much to handle, role stress is said to occur (Tubre & Collins, 2000). Role stress is typically broken down into three main types: role ambiguity, role conflict, and role overload. Role ambiguity refers to a situation in which employees are unsure about what their responsibilities are, what behaviors are expected of them, and what the standards are for …

Association Analyses Of Host Genetics, Root-Colonizing Microbes, And Plant Phenotypes Under Different Nitrogen Conditions In Maize, Michael A. Meier, Gen Xu, Martha G. Lopez-Guerrero, Guangyong Li, Christine Smith, Brandi Sigmon, Joshua R. Herr, James R. Alfano, Yufeng Ge, James C. Schnable, Jinliang Yang

Department of Agronomy and Horticulture: Faculty Publications

The root-associated microbiome (rhizobiome) affects plant health, stress tolerance, and nutrient use efficiency. However, it remains unclear to what extent the composition of the rhizobiome is governed by intraspecific variation in host plant genetics in the field and the degree to which host plant selection can reshape the composition of the rhizobiome. Here, we quantify the rhizosphere microbial communities associated with a replicated diversity panel of 230 maize (Zea mays L.) genotypes grown in agronomically relevant conditions under high N (+N) and low N (-N) treatments. We analyze the maize rhizobiome in terms of 150 abundant and consistently reproducible …

Spatial Stream Modeling Of Louisiana Waterthrush (Parkesia Motacilla) Foraging Substrate And Aquatic Prey In A Watershed Undergoing Shale Gas Development, Mack W. Frantz, Petra B. Wood, Steven C. Latta

United States Geological Survey: Staff Publications

We demonstrate the use of spatial stream network models (SSNMs) to explore relationships between a semiaquatic bioindicator songbird, Louisiana Waterthrush (Parkesia motacilla), and stream monitoring and benthic macroinvertebrate data in an area undergoing shale gas development. SSNMs allowed us to account for spatial autocorrelation inherent to these environmental data types and stream properties that traditional modeling approaches cannot capture to elucidate factors that affect waterthrush foraging locations. We monitored waterthrush along 58.1 km of 1st- and 2nd-order headwater stream tributaries (n = 14) in northwestern West Virginia over a two year period (2013–2014), sampled benthic macroinvertebrates in waterthrush …

Symbolic Annihilation And Stereotyping Of Native American Women In News: A Content Analysis Of Health, Safety, And Economic Status Related News, Shreyoshi Ghosh

College of Journalism and Mass Communications: Professional Projects

This is an exploratory study on the safety, economic, and health challenges of Native American women who constitute about 1.5% of the American population. With the symbolic annihilation and stereotyping of Native American people and women of color, there was a need to study the portrayal of Native American women in news. The findings indicated there was a growth in news coverage during 2018-19 and safety, including missing and murdered, emerged as a key topic. But symbolic annihilation in health and economic status including pay gap news was significant. Health news mostly covered maternal health and deaths but excluded most …

Social Connectedness In Schizotypy: The Role Of Cognitive And Affective Empathy, Jessica Stinson, Rebecca Wolfe, Will Spaulding

Department of Psychology: Faculty Publications

Social connectedness is increasingly understood to be a resilience factor that moderates vulnerability to poor physical and mental health. This study examines cognitive and affective processes that support normal socialization and social connectedness, and the impact of schizotypy, in well-functioning college students. In this study, a total of 824 college students completed a series of self-report questionnaires, and structural equation modeling was then employed to identify relationships between cognitive and affective empathy, alexithymia, distress tolerance, social connectedness, and schizotypy. Schizotypy is a trait-like condition, presumed to be genetic in origin, associated with the risk for schizophrenia. Like schizophrenia, schizotypy is …

Sweet Taste Signaling: The Core Pathways And Regulatory Mechanisms, Sunil Kumar Sukumaran, Salin Raj Palayyan

Department of Nutrition and Health Sciences: Faculty Publications

Sweet taste, a proxy for sugar-derived calories, is an important driver of food intake, and animals have evolved robust molecular and cellular machinery for sweet taste signaling. The overconsumption of sugar-derived calories is a major driver of obesity and other metabolic diseases. A fine-grained appreciation of the dynamic regulation of sweet taste signaling mechanisms will be required for designing novel noncaloric sweeteners with better hedonic and metabolic profiles and improved consumer acceptance. Sweet taste receptor cells express at least two signaling pathways, one mediated by a heterodimeric G-protein coupled receptor encoded by taste 1 receptor members 2 and 3 (TAS1R2 …

The Role Of Parental Health And Distress In Assessing Children’S Health Status, Sherrie H. Kaplan, Marilou Shaughnessy, Michelle A. Fortier, Marla Vivero‑Montemayor, Sergio Gago Masague, Dylan Hayes, Hal Stern, Maozhu Dai, Lauren Heim, Zeev Kain

Department of Psychology: Faculty Publications

Purpose The purpose of the study was to examine the contributions of parents’ health and distress to parent’s and children’s assessments of children’s health.

Methods We used baseline data from a longitudinal study of 364 children (ages 4–12) about to undergo surgery and their parents in a Southern California pediatric hospital. We used the 20-item child self-reported CHRIS 2.0 general health and the parallel parent-reported measure of the child’s health, along with a measure of parental distress about the child’s health were administered in the perioperative period. Other measures included parents’ physical and mental health, quality of life, distress over …

Implicit Trauma Identity Associations In Treatment-Seeking U.S. Military Personnel Do Not Predict Or Change In Response To Cognitive Processing Therapy For Ptsd, Kristen P. Lindgren, Anna E. Jaffe, Debra Kaysen, Bethany A. Teachman, Stacey Young-Mccaughan, Alan L. Peterson, Patricia A. Resick, Jennifer Schuster Wachen

Department of Psychology: Faculty Publications

Objective: This study evaluated implicit associations (i.e., associations in memory that are automatically activated and difficult to control consciously) related to trauma and one’s self in the context of a clinical trial for active duty service members seeking treatment for posttraumatic stress disorder (PTSD). Previous studies with nontreatment-seeking community samples found that implicit trauma identity associations were associated with PTSD symptoms even after controlling for amount of trauma exposure and self-reported negative cognitions about the self. This study extended prior work by evaluating whether trauma-related implicit associations were associated with PTSD and depressive symptoms in a clinical sample seeking …

Optimising Response To An Introduction Of African Swine Fever In Wild Pigs, Kim M. Pepin, Vienna R. Brown, Anni Yang, James C. Beasley, Raoul Boughton, Kurt C. Vercauteren, Ryan S. Miller, Sarah N. Bevins

United States Department of Agriculture Wildlife Services: Staff Publications

African swine fever virus (ASFv) is a virulent pathogen that threatens domestic swine industries globally and persists in wild boar populations in some countries. Persistence in wild boar can challenge elimination and prevent disease-free status, making it necessary to address wild swine in proactive response plans. In the United States, invasive wild pigs are abundant and found across a wide range of ecological conditions that could drive different epidemiological dynamics among populations. Information on the size of the control areas required to rapidly eliminate the ASFv in wild pigs and how this area should change with management constraints and local …

Nmr And Metabolomics—A Roadmap For The Future, David S. Wishart, Leo L. Cheng, Valérie Copié, Arthur S. Edison, Hamid R. Eghbalnia, Jeffrey C. Hoch, Goncalo J. Gouveia, Wimal Pathmasiri, Robert Powers, Tracey B. Schock, Lloyd W. Sumner, Mario Uchimiya

Department of Chemistry: Faculty Publications

Metabolomics investigates global metabolic alterations associated with chemical, biological, physiological, or pathological processes. These metabolic changes are measured with various analytical platforms including liquid chromatography-mass spectrometry (LC-MS), gas chromatographymass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR). While LC-MS methods are becoming increasingly popular in the field of metabolomics (accounting for more than 70% of published metabolomics studies to date), there are considerable benefits and advantages to NMR-based methods for metabolomic studies. In fact, according to PubMed, more than 926 papers on NMR-based metabolomics were published in 2021—the most ever published in a given year. This suggests that NMR-based metabolomics …

Replacing Fallow With Field Pea In Wheat Production Systems Across Western Nebraska, Samuel T. Koeshall, Amanda Easterly, Rodrigo Werle, Strahinja Stepanovic, Cody F. Creech

Department of Agronomy and Horticulture: Faculty Publications

Integration of field pea (Pisum sativum L.) (FP) into dryland cropping systems has increased due to ecological and economic benefits, paired with a growing market for pea-derived products. Challenges exist in the High Plains that limit the integration of crop rotations to replace fallow periods with FP in wheat (Triticum aestivum L.)- based systems. This experiment compares chemical summer fallow to FP in a fallow– wheat rotation at two locations in western Nebraska. Soil water content, soil fertility, N mineralization, FP yield, and subsequent hard red winter wheat (HWW) yields were recorded. Subsequent HWW yields were not different …

Profiling A Community-Specific Function Landscape For Bacterial Peptides Through Protein-Level Meta-Assembly And Machine Learning, Mitra Vajjala, Brady Johnson, Lauren Kasparek, Michael Leuze, Qiuming Yao

School of Computing: Faculty Publications

Small proteins, encoded by small open reading frames, are only beginning to emerge with the current advancement of omics technology and bioinformatics. There is increasing evidence that small proteins play roles in diverse critical biological functions, such as adjusting cellular metabolism, regulating other protein activities, controlling cell cycles, and affecting disease physiology. In prokaryotes such as bacteria, the small proteins are largely unexplored for their sequence space and functional groups. For most bacterial species from a natural community, the sample cannot be easily isolated or cultured, and the bacterial peptides must be better characterized in a metagenomic manner. The bacterial …

The Future Of Synbiotics: Rational Formulation And Design, David Fabian Gomez Gomez, Car Reen Kok, Robert Hutkins

Department of Food Science and Technology: Faculty Publications

Synbiotics, mixtures of live microbes and substrates selectively utilized by host organisms, are of considerable interest due to their ability to improve gastrointestinal health. However, formulating synbiotics remains challenging, due in part, to the absence of rational strategies to assess these products for synbiotic activities prior to clinical trials. Currently, synbiotics are formulated as either complementary or synergistic. Complementary synbiotics are made by combining probiotics and prebiotics, with each component acting independently and with the combination shown to provide a clinical health benefit. Most commercial synbiotics as well as those used in clinical trials have been of the complementary type. …

Immobilization Of A Bifidobacterial Endo-Ss-N-Acetylglucosaminidase To Generate Bioactive Compounds For Food Industry, Burcu Pekdemir, Hatice Duman, Ayşenur Arslan, Merve Kaplan, Melda Karyelioğlu, Tolgahan Özer, Hacı Mehmet Kayılı, Bekir Salih, Bethany M. Henrick, Rebbeca M. Duar, Sercan Karav

Department of Food Science and Technology: Faculty Publications

Conjugated N-glycans are considered next-generation bioactive prebiotic compounds due to their selective stimulation of beneficial microbes. These compounds are glycosidically attached to proteins through N-acetylglucosamines via specific asparagine residue (AsN-X-Ser/Thr). Certain bacteria such as Bifidobacterium longum subspecies infantis (B. infantis) have been shown to be capable of utilizing conjugated N-glycans, owing to their specialized genomic abilities. B. infantis possess a unique enzyme, Endo-ß-N-acetylglucosaminidase (EndoBI-1), which cleaves all types of conjugated N-glycans from glycoproteins. In this study, recombinantly cloned EndoBI- 1 enzyme activity was investigated using various immobilization methods: 1) adsorption, 2) entrapment-based alginate immobilization, …

Discrimination In The Employment Search: Narratives From International Students Of Color, Yi Xuen Tay

Department of Educational Administration: Dissertations, Theses, and Student Research

International students are “taking away jobs from Americans” (Citizenship and Immigration Services Ombudsman, 2020). Such a narrative popularized by the previous Trump administration positioned international students in the U.S. as threats to the country, and in this case, American workers. This narrative also targeted existing immigration policies, Optional Practical Training (OPT) and H-1B Specialty Occupations work visa, for allowing international students/nonimmigrants to work in the U.S. Yet, this narrative failed to account to the employment search experiences of international students, or international Students of Color, the subject of this study. While OPT and H-1B present as opportunities for international students, …

Construction And Operation Of A Pilot-Scale Odor Control Device, Brendan Bunker

Department of Environmental Engineering: Theses and Other Student Research

Hydrogen sulfide (H₂S) is a naturally occurring by-product of anaerobic digestion and causes an odor that can be a nuisance and a public health risk at higher concentrations. This compound can be treated through various means, but one of particular interest is a biotrickling filter (BTF). Such devices have excellent H₂S treatment efficiencies, but start-up costs can be steep. The primary goal of this study is to explain the design and operation of a low-cost odor control device for improving environmental air quality in Nebraska using a BTF. This device has the potential for application as …