Digital Commons Network^™

Co-Infection And Co-Localization Of Kaposi Sarcoma-Associated Herpesvirus And Epstein-Barr Virus In Hiv-Associated Kaposi Sarcoma: A Case Report, Peter Julius, Guobin Guobin, Stepfanie Siyumbwa, Jane Musumali, For Yue Tso, Owen Ngalamika, Trevor Kaile, Fred Maate, Phyllis Moonga, John T. West, Peter Angeletti, Charles Wood

Nebraska Center for Virology: Faculty Publications

Kaposi sarcoma (KS), a multifocal vascular neoplasm frequently observed in HIVpositive individuals, primarily affects the skin, mucous membranes, visceral organs, and lymph nodes. KS is associated primarily with Kaposi sarcomaassociated herpesvirus (KSHV) infection. In this case report, we present a rare occurrence of co-infection and co-localization of KSHV and Epstein-Barr virus (EBV) in KS arising from the conjunctiva, which, to our knowledge, has not been reported previously. Immunohistochemistry (IHC), DNA polymerase chain reaction (PCR), and EBV-encoded RNA in situ hybridization (EBER-ISH) were utilized to demonstrate the presence of KSHV and EBV infection in the ocular KS lesion. Nearly all KSHV-positive …

Rural District Leaders And Place In The Shadow Of The Pandemic: Refining The Conceptualization Of Leadership Of Place As Caring, Jeff Walls, Sarah Zuckerman

Department of Educational Administration: Faculty Publications

The COVID-19 pandemic heightened the tensions between rural community needs and politicized state-level school closure mandates. District leaders faced competing demands of meeting the basic needs of vulnerable families, supporting the mental health of students and teachers, protecting the health of all community members, and creating new opportunities for learning. This study examines how rural district leaders responded to these challenges through the lens of caring. This lens highlights how district leaders responded to their contexts, as well as their perceptions of student, family, and staff needs in ethically grounded and politically savvy ways. We draw on semi-structured interviews with …

Assessing The Toxicity Of Selected Pesticides To Trichogramma Ostriniae (Hymenoptera: Trichogrammatidae) Pupae As A First Step In The Development Of A Potential Novel Deployment Programme, Jeffrey D. Cluever, Clint W. Beiermann, Nevin C. Lawrence, Jeff Bradshaw

Panhandle Research and Extension Center

Trichogramma spp. have been used or have been proposed for use in biological control programmes of lepidopteran pests, including the European corn borer, Ostrinia nubilalis, the cotton bollworm, Helicoverpa armigera, and the spruce budworm, Choristoneura fumiferana. Releases are typically made by placing cards with parasitised eggs at set points in the field. However, this method can be cost-prohibitive due to its laborious nature. As a result, labour-saving mechanised release programmes have been developed, including distribution by spray equipment. However, few have investigated applying Trichogramma with a standard pesticide application (i.e. ‘tank mix’). As a first step to …

A Single-Dose Intramuscular Immunization Of Pigs With Lipid Nanoparticle Dna Vaccines Based On The Hemagglutinin Antigen Confers Complete Protection Against Challenge Infection With The Homologous Influenza Virus Strain, The N. Nguyen, Sushmita Kumari, Sarah Vitosh-Sillman, Jayeshbhai Chaudhari, Danh C. Lai, Hiep Vu

Nebraska Center for Virology: Faculty Publications

The Influenza A virus of swine (IAV-S) is highly prevalent and causes significant economic losses to swine producers. Due to the highly variable and rapidly evolving nature of the virus, it is critical to develop a safe and versatile vaccine platform that allows for frequent updates of the vaccine immunogens to cope with the emergence of new viral strains. The main objective of this study was to assess the feasibility of using lipid nanoparticles (LNPs) as nanocarriers for delivering DNA plasmid encoding the viral hemagglutinin (HA) gene in pigs. The intramuscular administration of a single dose of the LNP-DNA vaccines …

Statistical And Machine Learning Approaches To Describe Factors Affecting Preweaning Mortality Of Piglets, Md Towfiqur Rahman, Tami M. Brown-Bandl, Gary A. Rohrer, Sudhendu R. Sharma, Vamsi Manthena, Yeyin Shi

Department of Agricultural and Biological Systems Engineering: Faculty Publications

High preweaning mortality (PWM) rates for piglets are a significant concern for the worldwide pork industries, causing economic loss and well-being issues. This study focused on identifying the factors affecting PWM, overlays, and predicting PWM using historical production data with statistical and machine learning models. Data were collected from 1,982 litters from the U.S. Meat Animal Research Center, Nebraska, over the years 2016 to 2021. Sows were housed in a farrowing building with three rooms, each with 20 farrowing crates, and taken care of by well-trained animal caretakers. A generalized linear model was used to analyze the various sow, litter, …

Statistical And Machine Learning Approaches To Describe Factors Affecting Preweaning Mortality Of Piglets, Md Towfiqur Rahman, Tami M. Brown-Bandl, Gary A. Rohrer, Sudhendu R. Sharma, Vamsi Manthena, Yeyin Shi

Department of Agricultural and Biological Systems Engineering: Faculty Publications

High preweaning mortality (PWM) rates for piglets are a significant concern for the worldwide pork industries, causing economic loss and well-being issues. This study focused on identifying the factors affecting PWM, overlays, and predicting PWM using historical production data with statistical and machine learning models. Data were collected from 1,982 litters from the U.S. Meat Animal Research Center, Nebraska, over the years 2016 to 2021. Sows were housed in a farrowing building with three rooms, each with 20 farrowing crates, and taken care of by well-trained animal caretakers. A generalized linear model was used to analyze the various sow, litter, …

Bola3 And Nfu1 Link Mitoribosome Iron–Sulfur Cluster Assembly To Multiple Mitochondrial Dysfunctions Syndrome, Hui Zhong, Alexandre Janer, Oleh Khalimonchuk, Hana Antonicka, Eric A. Shoubridge, Antoni Barrientos

Department of Biochemistry: Faculty Publications

The human mitochondrial ribosome contains three [2Fe–2S] clusters whose assembly pathway, role, and implications for mitochondrial and metabolic diseases are unknown. Here, structure-function correlation studies show that the clusters play a structural role during mitoribosome assembly. To uncover the assembly pathway, we have examined the effect of silencing the e xpression of Fe–S cluster biosynthetic and delivery factors on mitoribosome stability. We find that the mitoribosome receives its [²Fe–2S] clusters from the GLRX5-BOLA3 node. Additionally, the assembly of the small subunit depends on the mitoribosome biogenesis factor METTL17, recently reported containing a [⁴Fe–4S] cluster, which we …

Milk-Borne Small Extracellular Vesicles: Kinetics And Mechanisms Of Transport, Distribution, And Elimination, Alice Ngu, Javaria Munir, Janos Zempleni

Department of Nutrition and Health Sciences: Faculty Publications

Small extracellular vesicles (sEVs) in milk have the qualities desired for delivering therapeutics to diseased tissues. The production of bovine milk sEVs is scalable (10²¹ annually per cow), and they resist degradation in the gastrointestinal tract. Most cells studied to date internalize milk sEVs by a saturable process that follows Michaelis-Menten kinetics. The bioavailability of oral milk sEVs is approximately 50%. In addition to crossing the intestinal mucosa, milk sEVs also cross barriers such as the placenta and blood-brain barrier, thereby enabling the delivery of therapeutics to hard-to-reach tissues. In time course studies, levels of milk sEVs peaked in …

Less Computer Access: Is It A Risk Or A Protective Factor For Cyberbullying And Face-To-Face Bullying Victimization Among Adolescents In The United States?, Jun Sung Hong, Miao Wang, Rekha Negi, Dexter R. Voisin, Lois M. Takahashi, Andre Iadipaolo

Department of Educational Psychology: Faculty Publications

The present study investigates whether less computer access is associated with an increase or decrease in cyberbullying and face-to-face bullying victimization. Data were derived from the 2009–2010 Health Behavior in School-Aged Children U.S. Study, consisting of 12,642 adolescents aged 11, 13, and 15 years (Mage = 12.95). We found that less computer usage was negatively associated with cyberbullying victimization and face-to-face bullying victimization. The findings from the study have implications for research and practice.

Dbapis: A Database Of Anti-Prokaryotic Immune System Genes, Yuchen Yan, Jinfang Zheng, Xinpeng Zhang, Yanbin Yin

Food for Health: Publications

Anti-prokary otic immune sy stem (APIS) proteins, typically encoded b y phages, prophages, and plasmids, inhibit prokaryotic immune systems (e.g. restriction modification, to xin-antito xin, CRISPR-Cas). A gro wing number of APIS genes ha v e been characterized and dispersed in the literature. Here w e de v eloped dbAPIS ( https:// bcb.unl.edu/ dbAPIS ), as the first literature curated data repository for experimentally verified APIS genes and their associated protein f amilies. T he k e y features of dbAPIS include: (i) e xperimentally v erified APIS genes with their protein sequences, functional annotation, PDB or AlphaFold predicted str …

Dbapis: A Database Of Anti-Prokaryotic Immune System Genes, Yuchen Yan, Jinfang Zheng, Xinpeng Zhang, Yanbin Yin

Department of Food Science and Technology: Faculty Publications

Anti-prokaryotic immune system (APIS) proteins, typically encoded b y phages, prophages, and plasmids, inhibit prokaryotic immune systems (e.g. restriction modification, to xin-antito xin, CRISPR-Cas). A growing number of APIS genes have been characterized and dispersed in the literature. Here we developed dbAPIS ( https:// bcb.unl.edu/ dbAPIS ), as the first literature curated data repository for experimentally verified APIS genes and their associated protein families. The key features of dbAPIS include: (i) experimentally verified APIS genes with their protein sequences, functional annotation, PDB or AlphaFold predicted structures, genomic context, sequence and structural homologs from different microbiome / virome databases; (ii) classification …

Assembly Of An Inexpensive Rat Jugular Catheter Button Based On A Split-Septum Needleless Intravenous System, Mauricio Suarez, Elizabeth J. Cantrell, Ken T. Wakabayashi, Caroline E. Bass

Department of Psychology: Faculty Publications

Rat intravenous self-administration is a widely-used animal model in the study of substance use disorders. Rats are tethered to a drug delivery system usually through a port or button that inter- faces the drug delivery system with a chronic indwelling jugular vein catheter. These buttons can be purchased commercially but are costly, presenting a significant economic barrier for many researchers. Many researchers manufacture buttons in-house from a combination of individual custom made and commercially available components, resulting in large variation in terms of how the animals are handled and the longevity of catheter patency. We have developed a jugular catheter …

Evaluation Of Land Preparation Methods For Soil Stabilization, Revegetation, And Renewed Productivity In Semi-Arid Climates, Jarrett Lardy, North Dakota State University, Miranda Meehan, Aaron L. M. Daigh, James Staricka, Nathan Derby

Department of Agronomy and Horticulture: Faculty Publications

In the Williston Basin, land-preparation treatments have not been compared in side-by-side trials to evaluate performance for parameters such as runoff, erosion, and vegetation establishment. Thus, four treatments of wheat-straw crimping, land imprinting, wood-fiber hydromulch, and the combination of land imprinting and hydromulch were evaluated against a bare soil control in a replicated and randomized completed block field experiment near Williston, ND. Rainfall simulations were performed in September 2020 and June 2021 to examine the effectiveness of the treatments to reduce runoff and sediment losses. Vegetation establishment was also evaluated in August 2021. The wheat straw treatment reduced the equivalent …

Kluyveromyces Marxianus Prepared As A Ready To Use Supplemental Food (Rusf), Zachary Christman

Applied Science Program: Theses

Ready to Use Supplemental Food (RUSF) is a nutrient dense paste or compressed bar used to supplement a person’s nutritional needs because of malnutrition or due to food shortages. The purpose of this article is to demonstrate some methods of how the dairy organism Kluyveromyces marxianus can be used to enrich the protein value of bread or ferment a substrate such as wheat bran into a more digestible form.

D-Shaped Nematode Eggs In The Feces Of Rangifer Tarandus: A Story In Pictures, Olga A. Loginova

MANTER: Journal of Parasite Biodiversity

D-shaped nematode eggs in the feces of Rangifer tarandus are expected to be oxyurid nematodes (Nemata: Oxyurida) of the genus Skrjabinema. The species S. tarandi is considered species-specific for this host. There is no consensus regarding the cross-infection of reindeer and sheep with S. ovis and S. tarandi. The drawings proposed by descriptors complicate differential diagnostics. Micrographs of S. tarandi eggs obtained via light microscopy and scanning electron microscopy as well as photographs of S. ovis eggs and drawings made on their basis are proposed to confidently distinguish between representatives of these two species, taking into account morphometric data. Thus, …

Astr 204

Department of Physics and Astronomy: Syllabi

No abstract provided.

Nebline, October 2023

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Super Fair Showcases 4-H’ers Learning Experiences

4-H Youth Use Videovoice to Address Health Equity

Recipe of the Month: Jajic Middle-Eastern Cucumber Salad by Emily Gratopp

American Bittersweet Brightens Up Fall by Sarah Browning

Garden Guide: Things to Do This Month

Helping Children Calm Down by Hayley Jackson

Adding Wheat to Your Corn-Soybean Rotation Has Many Benefifits by John Nelson

Heart of 4-H Volunteer Award: Kari Schepers

Be Part of Something Big! Join 4-H!

State 4-H Horse Expo Top Results

13-Year-Old Tenley Bauman Embraces 4-H Learning Experience at Fairs

4-H Announcements for 4-H'ers and Volunteers

Super Fair 4-H/FFA Top Results

State …

Specialized Late Cingulo-Opercular Network Activation Elucidates The Mechanisms Underlying Decisions About Ambiguity, Jordan E. Pierce, Nathan M. Petro, Elizabeth Clancy, Caterina Gratton, Steven E. Petersen, Maital Neta

Center for Brain, Biology, and Behavior: Faculty Publications

Cortical task control networks, including the cingulo-opercular (CO) network play a key role in decision-making across a variety of functional domains. In particular, the CO network functions in a performance reporting capacity that supports successful task performance, especially in response to errors and ambiguity. In two studies testing the contribution of the CO network to ambiguity processing, we presented a valence bias task in which masked clearly and ambiguously valenced emotional expressions were slowly revealed over several seconds. This slow reveal task design provides a window into the decision-making mechanisms as they unfold over the course of a trial. In …

Les Pollinator Habitat, Chloe Herbert, Dillon Richert, Melissa Landis

Environmental Engagement in the Community (ENVR 319): Student Projects

Environmental Engagement in the Community projects, fall 2023

A poster titled LES Pollinator Habitat for client Lincoln Electric System (C. Herbert and D. Richert with M. Landis).

Local Energy For Local Water: Developing Off-Grid Backup Power For Rural Communities In Nebraska, Brian Mock, Michael Ryan, Clement Konmegne Magaptche, James Persson

Environmental Engagement in the Community (ENVR 319): Student Projects

Environmental Engagement in the Community projects, fall 2023

A poster titled Local Energy for Local Water: Developing Off-grid Backup Power for Rural Communities in Nebraska for clients Nebraska local water operators (prepared by B. Mock, M. Ryan, and C. Konmegne Magaptche with J. Persson).

Lincoln Business Sustainability Assessment And Incentive Strategies For Emission Reduction, Jacson Farho, Moira Gonzales, Isabella Mccollum

Environmental Engagement in the Community (ENVR 319): Student Projects

Environmental Engagement in the Community projects, fall 2023

A poster titled Lincoln Business Sustainability Assessment and Incentive Strategies for Emission Reduction for client Coalition for Environmental Improvement (C. Eichorn and G. Hanlon).

Water Current, Volume 55, No. 3, Fall 2023

Water Current Newsletter

No abstract provided.

The Future Of U.S. Agricultural Biotechnology And Trade: Summary Of A Roundtable Discussion

Yeutter Institute Publications

The United States is one of the largest and highest-yield agricultural producers and exporters in the world. But the world’s population is growing, and growing conditions themselves are changing. Farms face shifting weather patterns, more frequent and severe storms, floods, and drought, and changing pest and disease pressures. What farmers plant and how they plant it are evolving to keep pace with these changes. Future global food security, sustainability, and U.S. leadership in agriculture will depend on the domestic and international policy and regulatory landscapes for agricultural biotechnology innovation. The Clayton Yeutter Institute of International Trade and Finance at the …

Phys 201

Department of Physics and Astronomy: Syllabi

No abstract provided.

Phys 441/841

Department of Physics and Astronomy: Syllabi

No abstract provided.

Gsl Researcher (Fall 2023)

GSL Researcher

GSL Researcher (Fall 2023)

Greetings

People

Range and pasture update

Technical note

Ranch update

Highlighted research

GSL Open House agenda

GSL spotlight

Nebraska Transcript (University Of Nebraska College Of Law), Fall 2023

Nebraska Transcript

Dean's message

Inclusive Leadership

Team earns $1M to bridge connections between history, civil rights

New student organizations highlight varied law student experiences

Inclusive Leadership Fellows guide 1L class 8 Bishop, ’92, named multicultural homecoming honoree

Mangram, ’24, named Inspire Scholarship Finalist

Student wellness initiatives expand

Ruiz, ’25, finds balance on and off the track

Advancing Justice Professor Danielle Jefferis: Protecting the Rights of the Incarcerated and Confined

Storer, ’17, works to combat human trafficking

2023 Strategic Plan Update

Inaugural Public Interest Scholars named

New clinic at Nebraska Law will defend First Amendment rights

Children’s Justice Clinic receives grant from Women …

Cultivating Capacity With A Mentoring Guild: Constellations Continued, Tracy W. Smith, Jennifer R. Luetkemeyer, Rachel Wilson, Aftynne E. Cheek, Denise Brewer

To Improve the Academy: A Journal of Educational Development

This article describes the rationale, development process, and initial activities and outcomes of a faculty mentoring guild that engages experienced faculty volunteers in supporting their near peers in navigating their teaching, research, service, and life expectations and obligations. The purposes of this article are to (1) describe the mentoring guild model; (2) provide a research-based rationale for a craft model of faculty support; (3) detail examples of mentoring guild activities, including the benefits and challenges; (4) recount explicit and implicit personal and professional outcomes for mentoring guild members; and (5) offer activities and lessons learned that faculty developers and institutions …

A Collaborative Model For Faculty Development: Helping Faculty Develop Inclusive Teaching Practices, Christina Bifulco, Christopher Drue

To Improve the Academy: A Journal of Educational Development

Inclusive pedagogy, an intentional approach to creating learning environments that meet the instructional needs of diverse student populations, is critically important and a moral imperative in contemporary higher education. Among the biggest challenges for creating faculty development programming to address the development and use of inclusive pedagogy are the lack of financial resources to support the effort and a lack of centralized institutional staff expertise and resources to inform and support such efforts. This case study gives a model for an institutional collaboration that incorporates experts from a variety of offices to deliver a series of inclusive teaching workshops and …

An Exploration Of Trauma-Inclusive Pedagogy And Students’ Perceptions Of Academic Success, R. Jason Lynch, Krista Wojdak

To Improve the Academy: A Journal of Educational Development

While trauma-inclusive approaches to student learning have been well documented in K–12 contexts, postsecondary education has done little to incorporate trauma-inclusive pedagogy into college classrooms. Using a sample (n = 529) of graduate and undergraduate students at a public rural-serving regional serving university, this study aims to explore differences in students’ perception of academic success in courses where trauma-inclusive practices were used and courses where these practices were not. Findings provide evidence that students felt more successful in courses where trauma-inclusive practices were used. Additionally, researchers were able to demonstrate that differences in perceptions of success were more pronounced …