Digital Commons Network^™

Lactic Acid Bacterial Surface Display Of Scytovirin Inhibitors For Anti-Ebolavirus Infection, Joshua Wiggins, Ngan Nguyen, Wenzhong Wei, Leah Liu Wang, Haley Hollingsead Olson, Shi-Hua Xiang

Nebraska Center for Virology: Faculty Publications

Scytovirin (SVN) is a lectin from cyanobacteria which has a strong inhibitory activity against Ebola virus infection. We engineered scytovirin as the inhibitor for surface display of lactic acid bacteria to block Ebola virus infection. Two different bacterial strains (Lactobacillus casei and Lactococcus lactis) were successfully engineered for scytovirin expression on the bacterial surface. These bacteria were found to be effective at neutralizing pseudotyped Ebolavirus in a cell-based assay. This approach can be utilized for prophylactic prevention, as well as for treatment. Since lactic acid bacteria can colonize the human body, a long-term efficacy could be achieved. Furthermore, …

Current Use Of Cigarettes In The United States: The Joint Role Of Race/Ethnicity And Health Insurance Coverage, Julia N. Soulakova, Lisa J. Crockett

Department of Psychology: Faculty Publications

The goal of this study was to assess the joint role of race/ethnicity and a health insurance coverage type (private, Medicare, Medicaid) in current cigarette use among adults in the U.S. Data from the 2019 Tobacco Use Supplement and the 2019 Annual Social and Economic Supplement of the Current Population Survey were merged (n = 39,882). Bivariate associations between each coverage type and smoking prevalence were examined within each of six racial/ethnic groups. A multiple logistic regression model (for the odds of current cigarette use) was estimated to explore the interactions between race/ethnicity and an indicator of each type of …

3dgaunet: 3d Generative Adversarial Networks With A 3d U-Net Based Generator To Achieve The Accurate And Effective Synthesis Of Clinical Tumor Image Data For Pancreatic Cancer, Yu Shi, Hannah Tang, Michael J. Baine, Michael A. Hollingsworth, Huijing Du, Dandan Zheng, Chi Zhang, Hongfeng Yu

School of Computing: Faculty Publications

Pancreatic ductal adenocarcinoma (PDAC) presents a critical global health challenge, and early detection is crucial for improving the 5-year survival rate. Recent medical imaging and computational algorithm advances offer potential solutions for early diagnosis. Deep learning, particularly in the form of convolutional neural networks (CNNs), has demonstrated success in medical image analysis tasks, including classification and segmentation. However, the limited availability of clinical data for training purposes continues to represent a significant obstacle. Data augmentation, generative adversarial networks (GANs), and cross-validation are potential techniques to address this limitation and improve model performance, but effective solutions are still rare for 3D …

Disruption Of Semiochemical-Mediated Movement By The Immature Trogoderma Variabile Baillon And Trogoderma Inclusum Le Conte (Coleoptera: Dermestidae) After Exposure To Long-Lasting Insecticide-Incorporated Netting, Sabita Ranabhat, Michael J. Domingue, Luke Lebar, Georgina Bingham, Kun Yan Zhu, William R. Morrison Iii

Department of Entomology: Faculty Publications

BACKGROUND: Highly mobile stored product insects may be able to readily orient in response to food cues and pheromones to attack durable commodities at each link of the postharvest supply chain. A 0.4% deltamethrin-incorporated long-lasting insecticide-incorporated netting (LLIN) is a successful novel preventative integrated pest management (IPM) tactic to intercept dispersing insects after harvest. However, it is unknown whether exposure to LLIN may affect olfaction and orientation to important semiochemicals by immature stored product dermestids, therefore the aim of this study was to assess whether exposure to LLIN disrupts the normal olfactory and chemotactic behavior of warehouse beetle, Trogoderma variabile …

Mechanisms And Opportunities For Rational In Silico Design Of Enzymes To Degrade Per- And Polyfluoroalkyl Substances (Pfas), Melissa Marciesky, Diana S. Aga, Ian M. Bradley, Nirupam Aich, Carla Ng

Department of Civil and Environmental Engineering: Faculty Publications

Per and polyfluoroalkyl substances (PFAS) present a unique challenge to remediation techniques because their strong carbon−fluorine bonds make them difficult to degrade. This review explores the use of in silico enzymatic design as a potential PFAS degradation technique. The scope of the enzymes included is based on currently known PFAS degradation techniques, including chemical redox systems that have been studied for perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) defluorination, such as those that incorporate hydrated electrons, sulfate, peroxide, and metal catalysts. Bioremediation techniques are also discussed, namely the laccase and horseradish peroxidase systems. The redox potential of known reactants and …

Tulathromycin Metaphylaxis Increases Nasopharyngeal Isolation Of Multidrug Resistant Mannheimia Haemolytica In Stocker Heifers, William B. Crosby, Brandi B. Karisch, Lari M. Hiott, Lee J. Pinnell, Alexandra Pittman, Jonathan G. Frye, Charlene R. Jackson, John Dustin Loy, William B. Epperson, John Blanton Jr., Sarah F. Capik, Paul S. Morley, Amelia R. Woolums

School of Veterinary and Biomedical Sciences: Faculty Publications

Bovine respiratory disease (BRD) is a leading cause of disease in feedlot and stocker calves with Mannheimia haemolytica (MH) as one of the most common etiologies. One of the most effective means of controlling BRD is through metaphylaxis, which involves administering antimicrobials to all animals at high risk of developing BRD. However, increasing prevalence of multidrug resistant (MDR) MH may reduce efficacy of metaphylaxis due to decreased susceptibility to drugs used for metaphylaxis. Primarily, this study aimed to determine the effect of tulathromycin metaphylaxis and subsequent BRD treatment on antimicrobial resistance (AMR) in MH isolated from stocker calves. Secondary objectives …

Root-Associated Bacterial Communities And Root Metabolite Composition Are Linked To Nitrogen Use Efficiency In Sorghum, Yen Ning Chai, Yunhui Qi, Emily Goren, Dawn Chiniquy, Amy M. Sheflin,, Susannah G. Tringe, Jessica E. Prenni, Peng Liu, Daniel P. Schachtman

Department of Agronomy and Horticulture: Faculty Publications

The development of cereal crops with high nitrogen use efficiency (NUE) is a priority for worldwide agriculture. In addition to conventional plant breeding and genetic engineering, the use of the plant microbiome offers another approach to improving crop NUE. To gain insight into the bacterial communities associated with sorghum lines that differ in NUE, a field experiment was designed comparing 24 diverse Sorghum bicolor lines under sufficient and deficient nitrogen (N). Amplicon sequencing and untargeted gas chromatography–mass spectrometry were used to characterize the bacterial communities and the root metabolome associated with sorghum genotypes varying in sensitivity to low N. We …

Radiofrequency Inactivation Of Salmonella In Black Pepper And Dried Basil Leaves Using In-Package Steaming, Surabhi Wason, Rossana Villa-Rojas, Jeyamkondan Subbiah

Department of Food Science and Technology: Faculty Publications

Radiofrequency (RF) heating has been extensively studied for pasteurizing low‐moisture foods. Currently, bulk foods are treated with radiofrequency; potential cross‐contamination may occur during packaging of pasteurized products. As an alternative, in‐package RF processing was evaluated for Salmonella inactivation on black peppercorns and dried basil leaves and prevention of cross‐contamination during storage postprocessing. Inpackage steaming refers to the process in which the samples were heated in a steam vent package to generate and retain steam during the treatment. This treatment achieved good heating uniformity which could be because of the circulation of steam within the package. One‐way steam vent allowed the …

Pathogenicity In Chickens And Turkeys Of A 2021 United States H5n1 Highly Pathogenic Avian Influenza Clade 2.3.4.4b Wild Bird Virus Compared To Two Previous H5n8 Clade 2.3.4.4 Viruses, Mary J. Pantin-Jackwood, Erica Spackman, Christina Leyson, Sungsu Youk, Scott A. Lee, Linda M. Moon, Mia K. Torchetti, Mary L. Killian, Julianna B. Lenoch, Darrell R. Kapczynski, David E. Swayne, David L. Suarez

United States Department of Agriculture Wildlife Services: Staff Publications

Highly pathogenic avian influenza viruses (HPAIVs) of subtype H5 of the Gs/GD/96 lineage remain a major threat to poultry due to endemicity in wild birds. H5N1 HPAIVs from this lineage were detected in 2021 in the United States (US) and since then have infected many wild and domestic birds. We evaluated the pathobiology of an early US H5N1 HPAIV (clade 2.3.4.4b, 2021) and two H5N8 HPAIVs from previous outbreaks in the US (clade 2.3.4.4c, 2014) and Europe (clade 2.3.4.4b, 2016) in chickens and turkeys. Differences in clinical signs, mean death times (MDTs), and virus transmissibility were found between chickens and …

An Analysis Of Racially Disparate Impacts On Housing For The City Of Tukwila, Washington, Neil Tabor

Community and Regional Planning Program: Professional Projects

The housing crisis within the Pacific Northwest of the United States has prompted action from Washington legislators, resulting in Governor Inslee identifying housing as a key priority and the legislature passing a number of forward-thinking bills in the last half decade, requiring the allocation of more resources, and asking more of jurisdictions to address current housing needs and anticipated future growth. Layered within the planning efforts for future growth are concerns and considerations for the potential of new housing development to perpetuate and exacerbate societal inequities built on discriminatory housing policies and practices of generations past and present. In an …

University Of Nebraska-Lincoln Agricultural Research Division 121st Annual Report. July 1, 2006 To June 30, 2007., Institute Of Agriculture And Natural Resources

Agricultural Research Division: News and Annual Reports

Our Mission..... 4

Foreword..... 5

Research Highlights..... 6

Faculty Awards and Recognitions....14

Graduate Student Awards and Recognitions...17

Undergraduate Honors Student Research Program...22

Variety and Germplasm Releases....23

Patents.....24

Administration.....25

Administrative Personnel....25

Organizational Chart....26

Administrative Units....27

IANR Research Facilities....28

Faculty.....29

Agricultural/Natural Resources Units....30

Education and Human Sciences Departments...39

Off-Campus Research Centers....40

Interdisciplinary Activities....41

Visiting Scientists/Research Associates....42

Research Projects.....47

Agricultural/Natural Resources Units....47

Education and Human Sciences Departments...52

Off-Campus Research Centers....52

Interdisciplinary Activities....53

Publications.....55

Agricultural/Natural Resources Units....60

Education and Human Sciences Departments...82

Off-Campus Research Centers....85

Research Expenditures....88

Analyzing Major Elements Of Crash Injury Severity Involving Priority-I Detriments Of Vision-Zero Plan, Muhammad Umer Farooq, Cole Madson, Aemal Khattak

Mid-America Transportation Center: Final Reports and Technical Briefs

Road traffic crashes result in significant economic losses for individuals, their families, and entire nations. These losses stem from the expenses associated with injury treatment, as well as the productivity lost due to fatalities or disabilities caused by these injuries. The 2030 Agenda for Sustainable Development set an ambitious target of halving the global number of fatalities and injuries from road traffic crashes by 2020 and achieving zero deaths by 2030, commonly referred to as the 'Target Zero Plan.' The Target Zero Plan prioritizes traffic safety issues into three distinct levels. The three priority levels are determined based on the …

Integrated Flood Risk Assessment Of Properties And Associated Population At County Scale For Nebraska, Usa, Shivendra Srivastava, Tirthankar Roy

Department of Civil and Environmental Engineering: Faculty Publications

Risk assessment of properties and associated population was conducted for the state of Nebraska, leveraging only open-source datasets. The flood risk framework consisted of interactions among drivers, i.e. hazard, exposure, vulnerability, and response, to assess the risks related to properties and associated populations. To quantify hazard on a county scale, we considered properties at risk of flooding based on a flood score (a higher score represents a greater chance of flooding). Exposure was quantified by considering population density at the county level. We quantified vulnerability under four categories: social, ecological, economic, and health. Response, a relatively newer component in flood …

Integrated Flood Risk Assessment Of Properties And Associated Population At County Scale For Nebraska, Usa, Shivendra Srivastava, Tirthankar Roy

Department of Civil and Environmental Engineering: Faculty Publications

Risk assessment of properties and associated population was conducted for the state of Nebraska, leveraging only open-source datasets. The flood risk framework consisted of interactions among drivers, i.e. hazard, exposure, vulnerability, and response, to assess the risks related to properties and associated populations. To quantify hazard on a county scale, we considered properties at risk of flooding based on a flood score (a higher score represents a greater chance of flooding). Exposure was quantified by considering population density at the county level. We quantified vulnerability under four categories: social, ecological, economic, and health. Response, a relatively newer component in flood …

Factors Affecting Forage Quality And The Subsequent Response In Production And Energy Metabolism In Lactating Jersey Cows, Kassidy Kate Buse

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Forage quality is the potential of a forage to produce a production response in animals. Because of how essential they are in dairy cattle rations, forages can have major influence on the behavior and production of dairy cattle. In experiment 1, the effect of forage fragility on the rumen environment and digestibility was evaluated. Increasing the inclusion of a less fragile forage led to greater rumen retention time, which resulted in lower rumen pH and greater NDF digestibility. Experiment 2 evaluated the effects of diets high in either corn silage or alfalfa haylage supplemented with either corn- or soy-based protein …

Raccoon Spatial Ecology In The Rural Southeastern United States, Jacob E. Hill, Madison L. Miller, James L. Helton, Richard B. Chipman, Amy Gilbert, University Of Georgia, Guha Dharmarajan, Olin E. Rhodes Jr.

United States Department of Agriculture Wildlife Services: Staff Publications

The movement ecology of raccoons varies widely across habitats with important implications for the management of zoonotic diseases such as rabies. However, the spatial ecology of raccoons remains poorly understood in many regions of the United States, particularly in the southeast. To better understand the spatial ecology of raccoons in the southeastern United States, we investigated the role of sex, season, and habitat on monthly raccoon home range and core area sizes in three common rural habitats (bottomland hardwood, upland pine, and riparian forest) in South Carolina, USA. From 2018–2022, we obtained 264 monthly home ranges from 46 raccoons. Mean …

Environmental Sustainability Of Small Wastewater Treatment Systems And Pathways For Improvement, Matthew James Thompson

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Wastewater treatment protects society and the environment by reducing pollutants generated from communities prior to discharge into local water bodies. Small water resource recovery facilities (WRRFs) represent the majority of centralized wastewater treatment systems, are resource intensive, and often lack resources needed to evaluate sustainability improvements. The goal of this dissertation was to identify primary sources and drivers of environmental impacts in small WRRFs and exploration of pathways to improve their environmental sustainability.

Case study data collected from small WRRFs in Nebraska was analyzed using environmental life cycle assessment (LCA) to quantify the environmental impact of facilities and existing opportunities. …

Rates Of Recent Adverse Childhood Experiences Among Indigenous Children, Emily A. Waterman, Katie Edwards, Natira Mullet, Ramona Herrington, Skyler Hopfauf, Preciouse Trujillo, Naomi Even-Aberle, Lorey Wheeler

Department of Educational Psychology: Faculty Publications

The current paper describes rates of recent (past six months) adverse childhood experiences (ACEs) and examines the association of ACEs with cultural connection and depressive symptoms among Indigenous children aged 10 to 14 (N = 177; mean age = 11.8; 48.3% boys; 44.3% girls; 7.4% another gender identity). Children completed baseline surveys as part of a larger evaluation of a culturally grounded, strengths-focused, family-based program to prevent ACEs. Surveys included an inclusive measure of ACEs developed for the current study, an adapted measure of connection to culture, and the Children’s Depression Screener. Results for ACEs indicated that 18.6% of …

Raccoon Spatial Ecology In The Rural Southeastern United States, Jacob E. Hill, Madison L. Miller, James L. Helton, Richard B. Chipman, Amy T. Gilbert, James C. Beasley, Guha Dharmarajan, Olin E. Rhodes Jr.

United States Department of Agriculture Wildlife Services: Staff Publications

The movement ecology of raccoons varies widely across habitats with important implications for the management of zoonotic diseases such as rabies. However, the spatial ecology of raccoons remains poorly understood in many regions of the United States, particularly in the southeast. To better understand the spatial ecology of raccoons in the southeastern US, we investigated the role of sex, season, and habitat on monthly raccoon home range and core area sizes in three common rural habitats (bottomland hardwood, upland pine, and riparian forest) in South Carolina, USA. From 2018–2022, we obtained 264 monthly home ranges from 46 raccoons. Mean monthly …

Covid‑19‑Related Risk, Resilience, And Mental Health Among Mexican American Mothers Across The First Year Of The Pandemic, Amy L. Non, Elizabeth S. Clausing, Sandraluz Lara‑Cinisomo, Kimberly L. D’Anna Hernandez

Center for Brain, Biology, and Behavior: Faculty Publications

Background Latina mothers have been especially affected by the pandemic and historically exhibit high rates of depression and anxiety. However, few longitudinal studies have assessed the effect of the pandemic on this vulnerable population. We hypothesized that COVID-19-related stressors would associate with psychological distress among Latina mothers across the first year of the pandemic.

Methods We investigated COVID-19-related impact, stigma, and fears across two critical time points and changes in these measures in relation to changes in maternal anxiety and depression among mothers of Mexican descent living in Southern California (n=152). Surveys were administered within 5–16 weeks of …

Value-Added Green Processing Of Tomato Waste To Obtain A Stable Free-Flowing Powder Lycopene Formulation Using Supercritical Fluid Technology, Purlen Sezer Okur, Ozan Ciftci

Department of Food Science and Technology: Faculty Publications

The tomato processing industry generates large amounts of waste, which creates disposal problems and negatively impacts the environment. Tomato waste contains lycopene, high-value health- and wellness-promoting bioactive, and it is a potential source of natural lycopene for food and nutraceutical applications. However, isolation of lycopene from its source requires toxic solvents, and lycopene is unstable during storage. The objective of this study was to extract lycopene from tomato waste and to convert it to a shelf-stable and easy-to-use free-flowing powder form using green extraction and particle formation processes based on supercritical carbon dioxide (SC-CO2) technology. Lycopene was extracted using SC-CO2 …

Organic Electronics In Biosensing: A Promising Frontier For Medical And Environmental Applications, Jyoti Bala Kaushal, Pratima Raut, Sanjay Kumar

Durham School of Architectural Engineering and Construction: Faculty Publications

The promising field of organic electronics has ushered in a new era of biosensing technology, thus offering a promising frontier for applications in both medical diagnostics and environmental monitoring. This review paper provides a comprehensive overview of organic electronics’ remarkable progress and potential in biosensing applications. It explores the multifaceted aspects of organic materials and devices, thereby highlighting their unique advantages, such as flexibility, biocompatibility, and low-cost fabrication. The paper delves into the diverse range of biosensors enabled by organic electronics, including electrochemical, optical, piezoelectric, and thermal sensors, thus showcasing their versatility in detecting biomolecules, pathogens, and environmental pollutants. Furthermore, …

Onboarding Program Eastern Band Of Cherokee Indians - Final Summary, Quality Improvement Center For Workforce Development

Other QIC-WD Products

The Eastern Band of Cherokee Indians (EBCI) is a federally-recognized tribe. The Family Safety Program (FSP) is the child welfare program that serves the Eastern Band of Cherokee Indians, under the tribal Division of Public Health and Human Services. FSP was established in 2015 with around 10 staff members and has grown to about 60 staff, all located in one office in Cherokee, North Carolina. Since FSP was a relatively new agency when they began working with the Quality Improvement Center for Workforce Development (QIC-WD), turnover had not been a signficant problem, however, vacancies were a challenge. In October 2017, …

Frontline Job Redesign Louisiana - Final Summary, Quality Improvement Center For Workforce Development

Other QIC-WD Products

The Louisiana Department of Children and Family Services (DCFS) Child Welfare Division (CWD) is a state-operated child welfare system. Louisiana has 64 parishes statewide organized into nine regions. The local parish offices provide child protective service (CPS), family services (FS), foster care (FC), and adoption services. CWD has approximately 1,500 employees including about 640 frontline caseworkers. In 2017, when Louisiana applied to become a QIC-WD site, CWD estimated its turnover rate across the state to be 24%, with one region having a turnover rate of 51%, while another had a turnover rate of only 8%.

This video discusses the challenges …

Frontline Job Redesign Louisiana - Prevention Worker Role Cards, Quality Improvement Center For Workforce Development

Other QIC-WD Products

Lists the different tasks given to types of prevention workers. View the download file.

Frontline Job Redesign Louisiana - Permanency Worker Role Cards, Quality Improvement Center For Workforce Development

Other QIC-WD Products

View the download file.

Organizational Change Process Milwaukee - Final Summary, Quality Improvement Center For Workforce Development

Other QIC-WD Products

Organizational Change in Action

The Division of Milwaukee Child Protective Services (DMCPS) is a state-operated child welfare system and is the only county system in Wisconsin run by the state. The Division is housed under the Department of Children and Families and has just under 200 staff, including: administrative/managerial staff, initial assessment and access (hotline) frontline workers, and fiscal and operations support. In 2016, DMCPS had an external turnover rate of 37% and they applied to be a Quality Improvement Center for Workforce Development (QIC-WD) site, with the goal of strengthening their child welfare workforce.

When DMCPS started working with …

Organizational Change Process Milwaukee - Site Intervention Logic Model, Quality Improvement Center For Workforce Development

Other QIC-WD Products

Each QIC-WD site developed a logic model to serve as a visual representation of their selected intervention. All logic models included four main components: inputs, activities, outputs, and outcomes. Collectively, these demonstrate the resource and actions required to implement the program, as well as the associated result of changes anticipated through implementation of the program. The hypothesized relationships are represented by the pathways connecting the listed activities and anticipated outcomes.

Addressing Work-Related Traumatic Stress Nebraska - Final Summary, Quality Improvement Center For Workforce Development

Other QIC-WD Products

Building a Resilient Workforce

Nebraska Division of Child and Family Services (DCFS) is a state-operated child welfare system. The Division is housed in the Department of Health and Human services and is divided into five service areas. DCFS has about 400 frontline child welfare workers (Child and Family Services Specialists or CFSS), 70 supervisors, and 15 field administrators. In 2017, DCFS had an annual turnover rate of about 30%. They applied to be a QIC-WD site with the goal of strengthening their child welfare workforce.

When DCFS started working with the Quality Improvement Center for Workforce Development (QIC-WD), an Implementation …

Supportive Supervision And Resiliency Ohio - Summary, Quality Improvement Center For Workforce Development

Other QIC-WD Products

Ohio Department of Job and Family Services (ODJFS) is a state-led, county-administered child welfare system. Ohio’s 83 single-county agencies and two multi-county agencies are responsible for the delivery of child protective services and ongoing case management in Ohio’s 88 counties. In 2017, ODJFS had an annual turnover rate of about 27%. They applied to be a Quality Improvement Center for Workforce Development (QIC-WD) site with the goal of strengthening their child welfare workforce. When ODJFS started working with the QICWD, a Workforce Implementation Team (WIT) was established to participate in a needs assessment process, determine an intervention, and support implementation. …