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An Event-Level Evaluation Of Women’S Self-Medicated Drinking: The Role Of Sexual Assault Severity, Affect, And Drinking Motives, Cynthia A. Stappenbeck, Anna E. Jaffe, Jessica A. Blayney, Mitchell Kirwan, William H. George, Kelly Cue Davis Mar 2024

An Event-Level Evaluation Of Women’S Self-Medicated Drinking: The Role Of Sexual Assault Severity, Affect, And Drinking Motives, Cynthia A. Stappenbeck, Anna E. Jaffe, Jessica A. Blayney, Mitchell Kirwan, William H. George, Kelly Cue Davis

Department of Psychology: Faculty Publications

Objective.—Women with sexual assault (SA) histories report heavier and more frequent drinking. Consistent with the motivational model of alcohol use, women with SA histories may consume alcohol to both down-regulate negative emotions and up-regulate positive ones. The present event-level study used a Bayesian multilevel moderated mediation approach to examine the extent to which women’s alcohol use and intoxication was influenced by coping and enhancement drinking motives to down-regulate or up-regulate affect, respectively.

Method.—Women ages 21–30 were recruited from the community to participate in a larger study that included a 32-day daily diary assessment of affect, drinking motives, and …


Influence Of Maternal Supplementation With Vitamins, Minerals, And (Or) Protein/Energy On Placental Development And Angiogenic Factors In Beef Heifers During Pregnancy, Bethania J. Dávila Ruiz, Carl R. Dahlen, Kacie L. Mccathy, Joel S. Caton, Jennifer L. Hurlbert, Friederike Baumgaertner, Ana Clara B. Menezes, Wellison J. S. Diniz, Sarah R. Underdahl, James D. Kirsch, Kevin K. Sedivec, Kerri A. Bochantin, Pawel P. Borowicz, Sebastián Canovas, Lawrence P. Reynolds Mar 2024

Influence Of Maternal Supplementation With Vitamins, Minerals, And (Or) Protein/Energy On Placental Development And Angiogenic Factors In Beef Heifers During Pregnancy, Bethania J. Dávila Ruiz, Carl R. Dahlen, Kacie L. Mccathy, Joel S. Caton, Jennifer L. Hurlbert, Friederike Baumgaertner, Ana Clara B. Menezes, Wellison J. S. Diniz, Sarah R. Underdahl, James D. Kirsch, Kevin K. Sedivec, Kerri A. Bochantin, Pawel P. Borowicz, Sebastián Canovas, Lawrence P. Reynolds

Department of Animal Science: Faculty Publications

The effect of vitamins and minerals supplementation (VTM) and/or two rates of body weight gain (GAIN) on bovine placental vascular development and angiogenic factors gene expression were evaluated in two experiments: In Exp. 1, crossbred Angus heifers (n = 34) were assigned to VTM/NoVTM treatments at least 71 days before breeding to allow changes in the mineral status. At breeding, through artificial insemination (AI), heifers were assigned to low-gain (LG) 0.28 kg/d or moderate-gain (MG) 0.79 kg/d treatments, resulting in NoVTM-LG (Control; n = 8), NoVTM-MG (n = 8), VTM-LG (n = 9), and VTM-MG (n = …


The Casnr L.I.N.K.S. Newsletter: Learning Innovation Network For K-12 Schools, Volume 3, Edition 3, March 2024, Bailey Feit, Tammy Mittelstet Mar 2024

The Casnr L.I.N.K.S. Newsletter: Learning Innovation Network For K-12 Schools, Volume 3, Edition 3, March 2024, Bailey Feit, Tammy Mittelstet

CASNR L.I.N.K.S. Newsletter

The purpose of this L.I.N.K.S. newsletter is to provide a monthly update of opportunities and resources for K-12 schools to connect with the University of Nebraska's CASNR Food, Energy, Water, and Societal Systems (FEWS²) programs and partners.

Included in this edition:

K-12 curriculum and resources

CASNR Ag Tech Day, April 23

2024 Summer Soybean Institute, May 29-30 and July 16-18

Youth Energy Leadership Camp July 22-26

Other upcoming events

Analytic investigators spotlight: UNL Statstics, Forensic Science, Ag Economics

Educators workshops

Student opportunities including NAYI, Native American Coalition, Cultivando Caminos, Big Red Camps, and more

Teacher opportunities including Economic tidbits from …


A Novel System To Culture Human Intestinal Organoids Under Physiological Oxygen Content To Study Microbial-Host Interaction, Tatiana Y. Fofanova, Umesh C. Karandikar, Jennifer M. Auchtung, Reid L. Wilson, Antonio J. Valentin, Robert A. Britton, K. Jane Grande-Allen, Mary K. Estes, Kristi Hoffman, Sashirekha Ramani, Christopher J. Stewart, Joseph F. Petrosino Mar 2024

A Novel System To Culture Human Intestinal Organoids Under Physiological Oxygen Content To Study Microbial-Host Interaction, Tatiana Y. Fofanova, Umesh C. Karandikar, Jennifer M. Auchtung, Reid L. Wilson, Antonio J. Valentin, Robert A. Britton, K. Jane Grande-Allen, Mary K. Estes, Kristi Hoffman, Sashirekha Ramani, Christopher J. Stewart, Joseph F. Petrosino

Food for Health: Publications

Mechanistic investigation of host-microbe interactions in the human gut are hindered by difficulty of co-culturing microbes with intestinal epithelial cells. On one hand the gut bacteria are a mix of facultative, aerotolerant or obligate anaerobes, while the intestinal epithelium requires oxygen for growth and function. Thus, a coculture system that can recreate these contrasting oxygen requirements is critical step towards our understanding microbial-host interactions in the human gut. Here, we demonstrate Intestinal Organoid Physoxic Coculture (IOPC) system, a simple and cost-effective method for coculturing anaerobic intestinal bacteria with human intestinal organoids (HIOs). Using commensal anaerobes with varying degrees of oxygen …


Nebline, March/April 2024 Mar 2024

Nebline, March/April 2024

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Seven Garden Gaffes to Avoid This Year by Sarah Browning

Youth Advance Health Equity with Community-Based Research by Emily Gratopp

Recipe of the Month: Crunchy Kohlrabi Salad by Emily Gratopp

GROBigRed Virtual Learning Series

Garden Guide: Things to Do This Month

2024 Lincoln Early Childhood Conference

2024 Chemigation Training

Corn Yield Challenge for 4-H and FFA Members

Lancaster County Ag VIP Awards

4-H Teens Work Together to Lead the Lock-In

4-H Horse/Livestock Judging Teams at Nationals

Heart of 4-H Volunteer Award: Sheridan Swotek and Kim Bowen

4-H Club Gives Youth With Disabilities Opportunity to Show Lambs

4-H Rabbit Show, March …


The Adverse Childhood Experiences Identification Gap In Speech Language Pathology, Mallory Prior Mar 2024

The Adverse Childhood Experiences Identification Gap In Speech Language Pathology, Mallory Prior

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Children exposed to adverse childhood experiences, a variety of potentially traumatic events occurring within the first 18 years of life, are at increased risk for speech and language disorders. Due to the high prevalence of trauma and its lasting effects, it is almost guaranteed that children who are experiencing the ongoing effects from adversity will be found on practicing Speech Language Pathologists’ caseloads. This scoping review was designed to identify current screening practices of Speech Language Pathologists (SLP) in comparison to other professionals (e.g., allied health and education), as well as additional information related to screening procedures for SLPs. The …


Pollinator Communities And Their Ecosystem Services At Conservation Grasslands And Adjacent Croplands, Araceli Gomez Villegas Mar 2024

Pollinator Communities And Their Ecosystem Services At Conservation Grasslands And Adjacent Croplands, Araceli Gomez Villegas

Department of Entomology: Dissertations, Theses, and Student Research

Pollinators are intrinsically linked to the success of unmanaged and managed ecosystems by providing pollination services that aid in the reproduction of wildflowers and many crops. Land use change, habitat loss, fragmentation, and related landscape-level phenomena (for example, increased pesticide exposure) threaten pollinators and have been associated with population declines. In the Midwestern region of the United States, land conversion of native prairies and grasslands to row-crop agriculture has been one of the largest contributors to pollinator habitat loss. Conservation programs, such as the Conservation Reserve Program, have worked towards removing environmentally sensitive lands from agriculture production and enrolling them …


The Great Resignation: Narratives Of Mid-Career Teacher Burnout And Attrition, Nicole Burton Mar 2024

The Great Resignation: Narratives Of Mid-Career Teacher Burnout And Attrition, Nicole Burton

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

The COVID-19 pandemic has increased the issues of teacher attrition. According to the U.S. Bureau of Labor and Statistics, from January to November 2021, there was a 40% increase in the quit levels of teachers within the state and local education agencies (John, 2022). While prior research has examined early-career teachers leaving the profession, mid-career teachers have largely been left out of the conversation. This narrative inquiry investigates the reasons that mid-career teachers are leaving the profession. Semi-structured interviews were conducted with four mid-career teachers hailing from the Midwest region. Interviews focused on understanding how teachers have experienced stress and …


Narratives Of Reproductive Control In The American Eugenics Movement, Cassandra M. Provost Mar 2024

Narratives Of Reproductive Control In The American Eugenics Movement, Cassandra M. Provost

Honors Program: Senior Projects (Public)

In this paper, I will explore the eugenics movement as a pseudo-scientific political, social, and legal phenomenon which had a devastating historical impact on America’s most vulnerable women, as well as briefly discuss its residual effects on contemporary reproductive rights conversations, through the lens of literature. Using an interdisciplinary discourse and narrative analysis approach, I identify two distinct themes within the explored narratives: (1) the importance of a government’s attempt to override a person’s autonomy by destroying the person’s ability to reproduce, and (2) the impropriety of actions based on a negative attitude toward disabled or undesirable persons. In my …


Familismo And Familial Support On The Psychological Effects Of Traumatic Event Exposure In Latinx Populations In The Midwest, Elizabeth Miller Mar 2024

Familismo And Familial Support On The Psychological Effects Of Traumatic Event Exposure In Latinx Populations In The Midwest, Elizabeth Miller

Honors Program: Senior Projects (Public)

Embedded within Latinx culture is Familismo, which serves as a source of strength and support for family members, while also shedding light on how familial support influences mental health. This study explores how Familismo and familial support impact the psychological effects of traumatic event exposure among Latinx populations in the Midwest. Analyzing data from 80 Latinx residents, I examined the relationship between familial support, traumatic event exposure, and mental health outcomes, focusing on depression and posttraumatic stress disorder (PTSD) symptoms. Results indicated a negative association between familial support and PTSD symptoms, indicating that higher support levels correlated with reduced PTSD …


Soil Nitrate Prediction Using Ftir-Atr, Vis-Nir, And Raman Spectroscopy, Sabiha Ferdous Mar 2024

Soil Nitrate Prediction Using Ftir-Atr, Vis-Nir, And Raman Spectroscopy, Sabiha Ferdous

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Fourier transform infrared spectroscopy (FTIR) combined with Attenuated total reflectance (ATR), Visible-Near Infrared spectroscopy (Vis-NIR), and Raman spectroscopy (RS) are non-destructive techniques for rapid determination of nitrogen compounds in soil. Leveraging FTIR-ATR and Vis-NIR spectra using partial least squares regression (PLSR) modeling, the study aims to predict soil nitrate content and explored the feasibility of Raman spectroscopy to detect nitrate (NO3-), nitrite (NO2-), and ammonium (NH4+) in soil. Soil samples were collected from four different fields, dried, sieved (2mm), and then used for collecting spectra (FTIR-ATR and Vis-NIR). Laboratory analysis was …


Cancer-Associated Fibroblast-Derived Acetate Promotes Pancreatic Cancer Development By Altering Polyamine Metabolism Via The Acss2–Sp1–Sat1 Axis, Divya Murthy, Kuldeep S. Attri, Surendra K. Shukla, Ravi Thakur, Nina V. Chaika, Chunbo He, Dezhen Wang, Kanupriya Jha, Aneesha Dasgupta, Ryan J. King, Scott E. Mulder, Joshua Souchek, Teklab Gebregiworgis, Vikant Rai, Rohit Patel, Tuo Hu, Sandeep Rana, Sai Sundeep Kollala, Camila Pacheco, Paul M. Grandgenett, Fang Yu, Vikas Kumar, Audrey J. Lazenby, Adrian R. Black, Susanna Ulhannan, Ajay Jain, Barish H. Edil, David L. Klinkebiel, Robert Powers, Amarnath Natarajan, Michael A. Hollingsworth, Kamiya Mehla2,, Quan Ly, Sarika Chaudhary, Rosa F. Hwang, Kathryn E. Wellen, Pankaj K. Singh Mar 2024

Cancer-Associated Fibroblast-Derived Acetate Promotes Pancreatic Cancer Development By Altering Polyamine Metabolism Via The Acss2–Sp1–Sat1 Axis, Divya Murthy, Kuldeep S. Attri, Surendra K. Shukla, Ravi Thakur, Nina V. Chaika, Chunbo He, Dezhen Wang, Kanupriya Jha, Aneesha Dasgupta, Ryan J. King, Scott E. Mulder, Joshua Souchek, Teklab Gebregiworgis, Vikant Rai, Rohit Patel, Tuo Hu, Sandeep Rana, Sai Sundeep Kollala, Camila Pacheco, Paul M. Grandgenett, Fang Yu, Vikas Kumar, Audrey J. Lazenby, Adrian R. Black, Susanna Ulhannan, Ajay Jain, Barish H. Edil, David L. Klinkebiel, Robert Powers, Amarnath Natarajan, Michael A. Hollingsworth, Kamiya Mehla2,, Quan Ly, Sarika Chaudhary, Rosa F. Hwang, Kathryn E. Wellen, Pankaj K. Singh

Robert Powers Publications

The ability of tumour cells to thrive in harsh microenvironments depends on the utilization of nutrients available in the milieu. Here we show that pancreatic cancer-associated fibroblasts (CAFs) regulate tumour cell metabolism through the secretion of acetate, which can be blocked by silencing ATP citrate lyase (ACLY) in CAFs. We further show that acetyl-CoA synthetase short-chain family member 2 (ACSS2) channels the exogenous acetate to regulate the dynamic cancer epigenome and transcriptome, thereby facilitating cancer cell survival in an acidic microenvironment. Comparative H3K27ac ChIP–seq and RNA–seq analyses revealed alterations in polyamine homeostasis through regulation of SAT1 gene expression and enrichment …


Rural Student-Home Community Engagement Impact As A Result Of Study Abroad Experiences: From Preparation Through Return, Todd M. Karr Mar 2024

Rural Student-Home Community Engagement Impact As A Result Of Study Abroad Experiences: From Preparation Through Return, Todd M. Karr

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This qualitative study utilized a constructivist grounded theory approach to understand how rural students engaged with people in their home community as it related to before, during, and after their study abroad experience and what impact that engagement had on their experiences. Participants included 15 students who self-identified as being from a rural background that studied abroad for a minimum of one semester. Participants included students from one of two large, public universities in the state of New Mexico. Data collection included two semi-structured, in-depth interviews with each participant.

The study used a constant comparison method to generate a theory …


Bermad: Batch Effect Removal For Single-Cell Rna-Seq Data Using A Multi-Layer Adaptation Autoencoder With Dual-Channel Framework, Xiangxin Zhan, Yanbin Yin, Han Zhang Feb 2024

Bermad: Batch Effect Removal For Single-Cell Rna-Seq Data Using A Multi-Layer Adaptation Autoencoder With Dual-Channel Framework, Xiangxin Zhan, Yanbin Yin, Han Zhang

Department of Food Science and Technology: Faculty Publications

Motivation: Removal of batch effect between multiple datasets from different experimental platforms has become an urgent problem, since single-cell RNA sequencing (scRNA-seq) techniques developed rapidly. Although there have been some methods for this problem, most of them still face the challenge of under-correction or over-correction. Specifically, handling batch effect in highly nonlinear scRNA-seq data requires a more powerful model to address under-correction. In the meantime, some previous methods focus too much on removing difference between batches, which may disturb the biological signal heterogeneity of datasets generated from different experiments, thereby leading to over-correction.

Results: In this article, we …


Relation Of Life Sciences Students’ Metacognitive Monitoring To Neural Activity During Biology Error Detection, Mei Grace Behrendt, Caron Clark, Mckenna Elliott, Joseph Dauer Feb 2024

Relation Of Life Sciences Students’ Metacognitive Monitoring To Neural Activity During Biology Error Detection, Mei Grace Behrendt, Caron Clark, Mckenna Elliott, Joseph Dauer

Department of Educational Psychology: Faculty Publications

Metacognitive calibration—the capacity to accurately self-assess one’s performance—forms the basis for error detection and self-monitoring and is a potential catalyst for conceptual change. Limited brain imaging research on authentic learning tasks implicates the lateral prefrontal and anterior cingulate brain regions in expert scientific reasoning. This study aimed to determine how variation in undergraduate life sciences students’ metacognitive calibration relates to their brain activity when evaluating the accuracy of biological models. Fifty undergraduate students enrolled in an introductory life sciences course completed a biology model error detection task during fMRI. Students with higher metacognitive calibration recruited lateral prefrontal regions linked in …


Psychological Distress And Behavioral Vigilance In Response To Minority Stress And Threat Among Members Of The Asian American And Pacific Islander Community During The Covid-19 Pandemic, Andrew S. Franks, Rin Nguyen, Y. Jenny Xiao, Dena Abbott Feb 2024

Psychological Distress And Behavioral Vigilance In Response To Minority Stress And Threat Among Members Of The Asian American And Pacific Islander Community During The Covid-19 Pandemic, Andrew S. Franks, Rin Nguyen, Y. Jenny Xiao, Dena Abbott

Department of Educational Psychology: Faculty Publications

Stigmatization, hostility, and violence towards the Asian American and Pacific Islander (AAPI) community have increased sharply during the COVID-19 pandemic. It is important to conduct research to promote understanding of the effects of such stigmatization on the AAPI community. Accordingly, the present study used a combined minority stress and integrated threat framework to examine whether factors related to AAPI identity would moderate the relationship between stigmatization/threat associated with AAPI identity and increased psychological distress and behavioral vigilance. AAPI individuals were recruited online from both Turk Prime and Reddit and completed measures of perceived stigmatization; integrated threat; depression, anxiety, and stress; …


Vitamin E Biofortification: Enhancement Of Seed Tocopherol Concentrations By Altered Chlorophyll Metabolism, Ping Qin, Peng Chen, Yuanwei Zhou, Wei Zhang, Yunyun Zhang, Jingjing Xu, Lu Gan, Yingnan Liu, Jill Romer, Peter Dörmann, Edgar B. Cahoon, Chunyu Zhang Feb 2024

Vitamin E Biofortification: Enhancement Of Seed Tocopherol Concentrations By Altered Chlorophyll Metabolism, Ping Qin, Peng Chen, Yuanwei Zhou, Wei Zhang, Yunyun Zhang, Jingjing Xu, Lu Gan, Yingnan Liu, Jill Romer, Peter Dörmann, Edgar B. Cahoon, Chunyu Zhang

Department of Biochemistry: Faculty Publications

Homogentisate Phytyltransferase (HPT) catalyzes condensation of homogentisate (HGA) and phytyl diphosphate (PDP) to produce tocopherols, but can also synthesize tocotrienols using geranylgeranyl diphosphate (GGDP) in plants engineered for deregulated HGA synthesis. In contrast to prior tocotrienol biofortification efforts, engineering enhanced tocopherol concentrations in green oilseeds has proven more challenging due to the integral role of chlorophyll metabolism in supplying the PDP substrate. This study show that RNAi suppression of CHLSYN coupled with HPT overexpression increases tocopherol concentrations by >two-fold in Arabidopsis seeds. We obtained additional increases in seed tocopherol concentrations by engineering increased HGA production via overexpression of …


African Swine Fever Virus Early Protein Pi73r Suppresses The Type-I Ifn Promoter Activities, Danh C. Lai, Jayeshbhai Chaudhari, Hiep L.X. Vu Feb 2024

African Swine Fever Virus Early Protein Pi73r Suppresses The Type-I Ifn Promoter Activities, Danh C. Lai, Jayeshbhai Chaudhari, Hiep L.X. Vu

Department of Animal Science: Faculty Publications

African swine fever virus is known to suppress type-I interferon (IFN) responses. The main objective of this study was to screen early-expressed viral genes for their ability to suppress IFN production. Out of 16 early genes examined, I73R exhibited robust suppression of cGAS-STING-induced IFN-β promoter activities, impeding the function of both IRF3 and NF-κB transcription factors. As a result, I73R obstructed IRF3 nuclear translocation following the treatment of cells with poly(dA:dT), a strong inducer of the cGAS-STING signaling pathway. Although the I73R protein exhibits structural homology with the Zα domain binding to the left-handed helical form of DNA known as …


Isothiocyanates Potentiate Tazemetostat-Induced Apoptosis By Modulating The Expression Of Apoptotic Genes, Members Of Polycomb Repressive Complex 2, And Levels Of Tri-Methylating Lysine 27 At Histone 3 In Human Malignant Melanoma Cells, Ioannis Anestopoulos, Ioannis Paraskevaidis, Sotiris Kyriakou, Lambrini E. Giova, Dimitrios T. Trafalis, Sotiris Botaitis, Rodrigo Franco, Aglaia Pappa, Mihalis L. Panayiotidis Feb 2024

Isothiocyanates Potentiate Tazemetostat-Induced Apoptosis By Modulating The Expression Of Apoptotic Genes, Members Of Polycomb Repressive Complex 2, And Levels Of Tri-Methylating Lysine 27 At Histone 3 In Human Malignant Melanoma Cells, Ioannis Anestopoulos, Ioannis Paraskevaidis, Sotiris Kyriakou, Lambrini E. Giova, Dimitrios T. Trafalis, Sotiris Botaitis, Rodrigo Franco, Aglaia Pappa, Mihalis L. Panayiotidis

School of Veterinary and Biomedical Sciences: Faculty Publications

In this study, we utilized an in vitro model consisting of human malignant melanoma as well as non-tumorigenic immortalized keratinocyte cells with the aim of characterizing the therapeutic effectiveness of the clinical epigenetic drug Tazemetostat alone or in combination with various isothiocyanates. In doing so, we assessed markers of cell viability, apoptotic induction, and expression levels of key proteins capable of mediating the therapeutic response. Our data indicated, for the first time, that Tazemetostat caused a significant decrease in viability levels of malignant melanoma cells in a dose- and time-dependent manner via the induction of apoptosis, while non-malignant keratinocytes were …


Growing Through Adversity: The Relation Of Early Childhood Educator Post-Traumatic Growth To Young Children’S Executive Function, Caron A. C. Clark, Holly Hatton-Bowers, Kimia Akhavein, Sarah Rasby, Gilbert R. Parra Feb 2024

Growing Through Adversity: The Relation Of Early Childhood Educator Post-Traumatic Growth To Young Children’S Executive Function, Caron A. C. Clark, Holly Hatton-Bowers, Kimia Akhavein, Sarah Rasby, Gilbert R. Parra

Department of Educational Psychology: Faculty Publications

Introduction: Early childhood educators (ECEs) play a critical role in supporting the development of young children’s executive functions (EF). EF, in turn, underpins lifelong resilience and well-being. Unfortunately, many ECEs report adverse childhood experiences (ACEs) that may compound high stress levels associated with an emotionally and physically demanding profession. ACEs have well-established negative implications for adult well-being and may dampen ECEs’ capacities to engage in emotionally responsive interactions with children. However, many individuals who experience ACEs also report post-traumatic growth experiences that foster empathy, self-determination, and resilience. Such post-traumatic growth may equip teachers with skills to engage in responsive …


Antibody Profiling And Predictive Modeling Discriminate Between Kaposi Sarcoma And Asymptomatic Kshv Infection, Sydney J. Bennett, Dicle Yalcin, Sara R. Privatt, Owen Ngalamika, Salum J. Lidenge, John T. West, Charles Wood Feb 2024

Antibody Profiling And Predictive Modeling Discriminate Between Kaposi Sarcoma And Asymptomatic Kshv Infection, Sydney J. Bennett, Dicle Yalcin, Sara R. Privatt, Owen Ngalamika, Salum J. Lidenge, John T. West, Charles Wood

School of Biological Sciences: Faculty Publications

Protein-level immunodominance patterns against Kaposi sarcoma-associated herpesvirus (KSHV), the aetiologic agent of Kaposi sarcoma (KS), have been revealed from serological probing of whole protein arrays, however, the epitopes that underlie these patterns have not been defined. We recently demonstrated the utility of phage display in high-resolution linear epitope mapping of the KSHV latency-associated nuclear antigen (LANA/ORF73). Here, a VirScan phage immunoprecipitation and sequencing approach, employing a library of 1,988 KSHV proteome-derived peptides, was used to quantify the breadth and magnitude of responses of 59 sub-Saharan African KS patients and 22 KSHV-infected asymptomatic individuals (ASY), and ultimately to support an application …


The Impact Of Iron Supplementation On The Preterm Neonatal Gut Microbiome: A Pilot Study, Matthew Van Ormer, Maranda Thompson, Melissa K. Thoene, Jean-Jack Riethoven, Sathish Kumar Natarajan, Ann Anderson-Berry Feb 2024

The Impact Of Iron Supplementation On The Preterm Neonatal Gut Microbiome: A Pilot Study, Matthew Van Ormer, Maranda Thompson, Melissa K. Thoene, Jean-Jack Riethoven, Sathish Kumar Natarajan, Ann Anderson-Berry

Department of Nutrition and Health Sciences: Faculty Publications

Objective The gastrointestinal microbiome in preterm infants exhibits significant influence on optimal outcomes–with dysbiosis shown to substantially increase the risk of the life-threatening necrotizing enterocolitis. Iron is a vital nutrient especially during the perinatal window of rapid hemoglobin production, tissue growth, and foundational neurodevelopment. However, excess colonic iron exhibits potent oxidation capacity and alters the gut microbiome–potentially facilitating the proliferation of pathological bacterial strains. Breastfed preterm infants routinely receive iron supplementation starting 14 days after delivery and are highly vulnerable to morbidities associated with gastrointestinal dysbiosis. Therefore, we set out to determine if routine iron supplementation alters the preterm gut …


A Systematic Survey On 5g And 6g Security Considerations, Challenges, Trends, And Research Areas, Paul Scalise, Matthew Boeding, Hamid Sharif, Joseph Delloiacovo, John Reed Feb 2024

A Systematic Survey On 5g And 6g Security Considerations, Challenges, Trends, And Research Areas, Paul Scalise, Matthew Boeding, Hamid Sharif, Joseph Delloiacovo, John Reed

Department of Electrical and Computer Engineering: Faculty Publications

With the rapid rollout and growing adoption of 3GPP 5thGeneration (5G) cellular services, including in critical infrastructure sectors, it is important to review security mechanisms, risks, and potential vulnerabilities within this vital technology. Numerous security capabilities need to work together to ensure and maintain a sufficiently secure 5G environment that places user privacy and security at the forefront. Confidentiality, integrity, and availability are all pillars of a privacy and security framework that define major aspects of 5G operations. They are incorporated and considered in the design of the 5G standard by the 3rd Generation Partnership Project (3GPP) with the goal …


Blood Lead, Cadmium And Hair Mercury Concentrations And Association With Soil, Dust And Occupational Factors In E-Waste Recycling Workers In Bangladesh, Sarker Masud Parvez, Farjana Jahan, Jaynal Abedin, Mahbubur Rahman, Shaikh Sharif Hasan, Nafisa Islam, Nirupam Aich, Mohammad Moniruzzaman, Zahir Islam, Masatake Fujimura, Rubhana Raqib, Luke D. Knibbs, Peter D. Sly Feb 2024

Blood Lead, Cadmium And Hair Mercury Concentrations And Association With Soil, Dust And Occupational Factors In E-Waste Recycling Workers In Bangladesh, Sarker Masud Parvez, Farjana Jahan, Jaynal Abedin, Mahbubur Rahman, Shaikh Sharif Hasan, Nafisa Islam, Nirupam Aich, Mohammad Moniruzzaman, Zahir Islam, Masatake Fujimura, Rubhana Raqib, Luke D. Knibbs, Peter D. Sly

Department of Civil and Environmental Engineering: Faculty Publications

Background: Electronic waste (e-waste) recycling activities release toxic metals, which pose substantial hazard to the environment and human health. We evaluated metal concentrations in biological and environmental samples, and examined the associations between biological lead (Pb), cadmium (Cd), and mercury (Hg) with soil and dust metals, and other possible determinants, among populations exposed and non-exposed to e-waste in Bangladesh.

Methods: A total of 199 e-waste workers and 104 non-exposed individuals were recruited. We measured blood Pb (BPb) and Cd (BCd) concentrations and total Hg (THg) from hair samples. Data were collected on occupational, and behavioral factors. We fitted …


Impact Of Covid‑19 On Food Security And Diet Quality In Chilanga District, Zambia, Shela Sridhar, Janella Kang, Joyce Makasa, Sally Bell‑Cross, Isabel Madzorera, Ethan Zulu, Davidson H. Hamer Feb 2024

Impact Of Covid‑19 On Food Security And Diet Quality In Chilanga District, Zambia, Shela Sridhar, Janella Kang, Joyce Makasa, Sally Bell‑Cross, Isabel Madzorera, Ethan Zulu, Davidson H. Hamer

Department of Child, Youth, and Family Studies: Faculty Publications

Introduction Food security and nutrition have been severely impacted during the COVID-19 pandemic, particularly in low- and middle-income countries (LMICs). We aimed to quantify the impacts of the pandemic on food security and diet diversity within Chilanga District in Zambia and identify target areas for high-impact social protection and safety net programs.

Methods We conducted a cross-sectional study in Chilanga district immediately after the Omicron variant surge in February 2022. Diet quality and food security were assessed based on a household diet questionnaire and a Minimum Dietary Diversity-Women (MDD-W) score was calculated. A paired t-test was used to determine whether …


Development Of The Quantitative Modelling Observation Protocol (Qmop) For Undergraduate Biology Courses: Validity Evidence For Score Interpretation And Uses, Lyrica Lucas, Anum Khushal, Robert Mayes, Brian Couch, Joseph Dauer Feb 2024

Development Of The Quantitative Modelling Observation Protocol (Qmop) For Undergraduate Biology Courses: Validity Evidence For Score Interpretation And Uses, Lyrica Lucas, Anum Khushal, Robert Mayes, Brian Couch, Joseph Dauer

School of Natural Resources: Faculty Publications

Educational reform priorities such as emphasis on quantitative modelling (QM) have positioned undergraduate biology instructors as designers of QM experiences to engage students in authentic science practices that support the development of datadriven and evidence-based reasoning. Yet, little is known about how biology instructors adapt to the pedagogical movement towards incorporating QM opportunities for students in the courses they teach. This study presents the development of the Quantitative Modelling Observation Protocol (QMOP), a classroom observation instrument designed to support the need to characterise various approaches that instructors use to implement QM instruction in undergraduate biology. QMOP provides information about the …


Disruption Of The Sorghum Circadian Clock Impacts Sorghum-Sugarcane Aphid Interaction Dynamics And Aphid Feeding Behavior, Kumar Shrestha, Prince Zogli, Lise Pingault, Sajjan Grover, Juan Betancurt Cardona, Joe Louis Feb 2024

Disruption Of The Sorghum Circadian Clock Impacts Sorghum-Sugarcane Aphid Interaction Dynamics And Aphid Feeding Behavior, Kumar Shrestha, Prince Zogli, Lise Pingault, Sajjan Grover, Juan Betancurt Cardona, Joe Louis

Department of Entomology: Faculty Publications

Circadian clocks play a pivotal role in orchestrating metabolic rhythms in response to environmental stress. Sorghum bicolor (sorghum), a multipurpose cereal crop, suffers severe growth and yield reduction due to feeding by sugarcane aphids (SCA; Melanaphis sacchari), which are phloem-feeding pests. Previously, it has been shown that sorghum utilizes a multitude of defense mechanisms to curb SCA colonization. However, our understanding of the impact of circadian clock on sorghum-aphid interaction dynamics is limited. To explore this, a time-series transcriptomics was conducted on sorghum plants in disrupted circadian rhythm with and without SCA infestation. Transcriptomic analysis revealed a total of …


A Systematic Review Of Fourth Industrial Revolution Technologies In Smart Irrigation: Constraints, Opportunities, And Future Prospects For Sub-Saharan Africa, Joshua Wanyama, Erion Bwambale, Shafik Kiraga, Abia Katimbo, Prossie Nakawuka, Isa Kabenge, Isaac Oluk Feb 2024

A Systematic Review Of Fourth Industrial Revolution Technologies In Smart Irrigation: Constraints, Opportunities, And Future Prospects For Sub-Saharan Africa, Joshua Wanyama, Erion Bwambale, Shafik Kiraga, Abia Katimbo, Prossie Nakawuka, Isa Kabenge, Isaac Oluk

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The adoption of Fourth Industrial Revolution (4IR) technologies has revolutionized agricultural practices worldwide. However, their application in the context of sub-Saharan Africa remains a critical challenge. This study presents a systematic review that investigates the potential of 4IR technologies in smart irrigation. Sub- Saharan Africa faces multiple agricultural challenges, exacerbated by climate change, water scarcity, and inefficient irrigation practices. The need for sustainable, water-efficient, and data-driven irrigation systems is urgent to ensure food security and economic development in the region. This study addresses a crucial knowledge gap by assessing the constraints, opportunities, and prospects of implementing 4IR technologies for smart …


Relationship Of Tissue Dimensions And Three Captive Bolt Application Sites On Cadaver Heads From Mature Swine (Sus Scrofa Domesticus) < 200 Kg Body-Weight, Karly N. Anderson, Ashlynn A. Kirk, Jennifer Berger, Arquimides A. Reyes, Ruth Woiwode, Perle E. Zhitnitskiy, Kurt D. Vogel Feb 2024

Relationship Of Tissue Dimensions And Three Captive Bolt Application Sites On Cadaver Heads From Mature Swine (Sus Scrofa Domesticus) < 200 Kg Body-Weight, Karly N. Anderson, Ashlynn A. Kirk, Jennifer Berger, Arquimides A. Reyes, Ruth Woiwode, Perle E. Zhitnitskiy, Kurt D. Vogel

Department of Animal Science: Faculty Publications

Penetrating captive bolt (PCB) is a common method of euthanasia for swine but has not been evaluated for mature swine < 200 kg body weight (BW). The objectives were to determine tissue depth, brain contact plane, and visible brain tissue damage (brain damage[BD]) for the common FRONTAL (F) and alternative TEMPORAL (T) and BEHIND EAR (BE) placements for PCB use on sows and boars weighing < 200 kg. Cadaver heads were obtained from 30 sows and 30 boars (estimated BW, mean ± SD; sows: 165.8 ± 22.4 kg; boars: 173.6 ± 21.4 kg) from a slaughter establishment after electrical stunning and exsanguination. Heads were cooled at 2 to 4 °C for approximately 64 h. A Jarvis PAS-Type P 0.25R PCB with a Long Stunning Rod Nosepiece Assembly and a 3.5 GR power load was used for all PCB applications at the following placements: F–3.5 cm superior to the optic orbits at midline, T–at the depression posterior to the lateral canthus of the eye within the plane between the lateral canthus and the base of the ear, or BE–directly caudal to the pinna of the ear on the same plane as the eyes and targeting the middle of the opposite eye. For sows, the bolt path was in the brain for 10/10 (100.0%, 95% CI: 69.2% to 100.0%) F, T, and BE heads. In heads that could reliably be assessed for BD, BD was detected in 10/10 (100.0%, 95% CI: 69.2% to 100.0%) F heads, 9/9 (100.0%, 95% CI: 66.4% to 100.0%) T heads, and 0/10 (0.0%, 95% CI: 0.0% to 30.1%) BE heads. For boars, the bolt path was in the plane of the brain for 8/9 (88.9%, 95% CI: 51.8% to 99.7%) F heads, 9/10 (90.0%, 95% CI: 55.5% to 99.7%) T heads, and 11/11 (100.0%, 95% CI: 71.5% to 100.0%) BE heads. In heads that could reliably be assessed for BD, BD was detected in 8/9 (88.9%, 95% CI: 51.7% to 99.7%) F heads, 7/10 (70.0%, 95% CI: 34.8% to 93.3%) T heads, and 4/11 (36.4%, 95% CI: 10.9% to 69.2%) BE heads. Tissue depth was reported as mean ± SE followed by 95% one-sided upper reference limit (URL). For sows, total tissue thickness differed (P < 0.05) between placements (F: 49.41 ± 2.74 mm, URL: 70.0 mm; T: 62.83 ± 1.83 mm, URL: 76.6 mm; BE: 84.63 ± 3.67 mm; URL: 112.3 mm). Total tissue thickness differed (P < 0.05) between placements for boars (F: 54.73 ± 3.23 mm, URL: 77.6 mm; T: 70.72 ± 3.60 mm, URL: 96.3 mm; BE: 92.81 ± 5.50 mm; URL: 135.3 mm). For swine between 120 and 200 kg BW, the F placement may have the greatest likelihood for successful euthanasia due to the least total tissue thickness and may present less risk for failure than the T and BE placements.


Hymenopteran Parasitoid Complex And Fall Armyworm: A Case Study In Eastern India, Subhajit Pal, Swarnali Bhattacharya, Tapamay Dhar, Ankita Gupta, Arunava Ghosh, Sandip Debnath, Nikhitha Gangavarapu, Prajna Pati, Nilanjana Chaudhuri, Hirak Chatterjee, Sabita Kumar Senapati, Prateek Madhab Bhattacharya, Mahesh Kumar Gathala, Alison M. Laing Feb 2024

Hymenopteran Parasitoid Complex And Fall Armyworm: A Case Study In Eastern India, Subhajit Pal, Swarnali Bhattacharya, Tapamay Dhar, Ankita Gupta, Arunava Ghosh, Sandip Debnath, Nikhitha Gangavarapu, Prajna Pati, Nilanjana Chaudhuri, Hirak Chatterjee, Sabita Kumar Senapati, Prateek Madhab Bhattacharya, Mahesh Kumar Gathala, Alison M. Laing

Department of Entomology: Faculty Publications

Fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith) has significantly affected maize crop yields, production efficiency, and farmers’ incomes in the Indian Eastern Gangetic Plains region since it was first observed in India in 2018. A lack of awareness by maize growers of the appropriate selection, method, and timing of insecticide application not only creates a barrier to sustainable FAW control but also contributes to increased environmental pollution, reduced human health and increased production costs. We demonstrated that FAW inflicted the most damage in early whorl growth stage of maize, regardless of whether chemical insecticides were applied. FAW egg masses and …