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Enhancing Urban Water Quality Through Biological-Chemical Treatment: Aquatic Macroinvertebrate Community And Temporal Chlorophyll-A Response, Matthew Chaffee Dec 2023

Enhancing Urban Water Quality Through Biological-Chemical Treatment: Aquatic Macroinvertebrate Community And Temporal Chlorophyll-A Response, Matthew Chaffee

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

With a growing human population, urbanization is impeding a plethora of natural waterways. Of these, urban ponds play a vital role in nutrient sequestration, flood prevention, and habitat sanctuaries. However, nutrient loading can reduce habitat effectiveness and promote harmful algae blooms. To reduce internal nutrient loads, a biological-chemical treatment strategy consisting of floating treatment wetlands (FTWs) and lanthanum were applied to two urban retention ponds, Densmore and Wilderness Ridge Ponds. To measure effectiveness, chlorophyll-a samples were collected and correlated with Sentinel-2. A novel band algorithm termed 3BR1 produced a strong correlation (R2 = 0.72) to physical chlorophyll-a …


Spatial Release From Masking In Anechoic And Reverberant Environments, Drake Andrew Hintz Dec 2023

Spatial Release From Masking In Anechoic And Reverberant Environments, Drake Andrew Hintz

Durham School of Architectural Engineering and Construction: Dissertations, Theses, and Student Research

Listening with both ears provides children with access to binaural and monaural cues that are helpful for understanding speech in competing babbles. Specifically, when the target and masker are spatially separated, children can gain an intelligibility benefit which is known as spatial release from masking (SRM). Recent work [Peng et al., 2021 JASA] suggested that school-age children demonstrated immature SRM using binaural cues that are distorted by reverberation. In this follow-up study, we further investigate the effect of reverberant distortion on individual auditory spatial cues, namely binaural and monaural head shadow cues. We compare SRM between adults and school-age children …


Early Childhood Language Gains, Kindergarten Readiness, And Grade 3 Reading Achievement, Jessica A.R. Logan, Shayne B. Piasta, Kelly M. Purtell, Robert Nichols, Rachel E. Schachter Dec 2023

Early Childhood Language Gains, Kindergarten Readiness, And Grade 3 Reading Achievement, Jessica A.R. Logan, Shayne B. Piasta, Kelly M. Purtell, Robert Nichols, Rachel E. Schachter

Department of Child, Youth, and Family Studies: Faculty Publications

In this preregistered study, we used latent change score models to address two research aims: (1) whether preschool-aged children's language gains, over a year of early childhood education, were associated with later performance on state-mandated, literacy-focused kindergarten readiness and Grade 3 reading achievement assessments, and (2) whether gains in language, a more complex skill, predicted these outcomes after controlling for more basic emergent literacy skills. There were 724 participating children (mean = 57 months; 51% male; 76% White, 12% Black, 6% multiple races, and 5% Hispanic or Latino). We found that language gains significantly predicted kindergarten readiness when estimated in …


Aicropcam: Deploying Classification, Segmentation, Detection, And Counting Deep-Learning Models For Crop Monitoring On The Edge, Nipuna Chamara, Geng (Frank) Bai, Yufeng Ge Dec 2023

Aicropcam: Deploying Classification, Segmentation, Detection, And Counting Deep-Learning Models For Crop Monitoring On The Edge, Nipuna Chamara, Geng (Frank) Bai, Yufeng Ge

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Precision Agriculture (PA) promises to meet the future demands for food, feed, fiber, and fuel while keeping their production sustainable and environmentally friendly. PA relies heavily on sensing technologies to inform site-specific decision supports for planting, irrigation, fertilization, spraying, and harvesting. Traditional point-based sensors enjoy small data sizes but are limited in their capacity to measure plant and canopy parameters. On the other hand, imaging sensors can be powerful in measuring a wide range of these parameters, especially when coupled with Artificial Intelligence. The challenge, however, is the lack of computing, electric power, and connectivity infrastructure in agricultural fields, preventing …


Determining The Presence And Size Of Shoulder Lesions In Sows Using Computer Vision, Shubham Bery, Tami M. Brown-Brandl, Bradley T. Jones, Gary A. Rohrer, Sudhendu Raj Sharma Dec 2023

Determining The Presence And Size Of Shoulder Lesions In Sows Using Computer Vision, Shubham Bery, Tami M. Brown-Brandl, Bradley T. Jones, Gary A. Rohrer, Sudhendu Raj Sharma

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Shoulder sores predominantly arise in breeding sows and often result in untimely culling. Reported prevalence rates vary significantly, spanning between 5% and 50% depending upon the type of crate flooring inside a farm, the animal’s body condition, or an existing injury that causes lameness. These lesions represent not only a welfare concern but also have an economic impact due to the labor needed for treatment and medication. The objective of this study was to evaluate the use of computer vision techniques in detecting and determining the size of shoulder lesions. A Microsoft Kinect V2 camera captured the top-down depth and …


Accelerated Evolution Of Sars-Cov-2 In Free-Ranging White-Tailed Deer, Dillon S. Mcbride, Sofya K. Garushyants, John Franks, Andrew F. Magee, Steven H. Overend, Devra Huey, Amanda M. Williams, Seth A. Faith, Ahmed Kandeil, Sanja Trifkovic, Lance Miller, Trushar Jeevan, Anami Patel, Jacqueline M. Nolting, Michael J. Tonkovich, J. Tyler Genders, Andrew J. Montoney, Kevin Kasnyik, Timothy J. Linder, Sarah N. Bevins, Julianna B. Lenoch, Jeffrey C. Chandler, Thomas J. Deliberto, Eugene V. Koonin, Marc A. Suchard, Philippe Lemey, Richard J. Webby, Martha I. Nelson, Andrew S. Bowman Dec 2023

Accelerated Evolution Of Sars-Cov-2 In Free-Ranging White-Tailed Deer, Dillon S. Mcbride, Sofya K. Garushyants, John Franks, Andrew F. Magee, Steven H. Overend, Devra Huey, Amanda M. Williams, Seth A. Faith, Ahmed Kandeil, Sanja Trifkovic, Lance Miller, Trushar Jeevan, Anami Patel, Jacqueline M. Nolting, Michael J. Tonkovich, J. Tyler Genders, Andrew J. Montoney, Kevin Kasnyik, Timothy J. Linder, Sarah N. Bevins, Julianna B. Lenoch, Jeffrey C. Chandler, Thomas J. Deliberto, Eugene V. Koonin, Marc A. Suchard, Philippe Lemey, Richard J. Webby, Martha I. Nelson, Andrew S. Bowman

United States Department of Agriculture Wildlife Services: Staff Publications

The zoonotic origin of the COVID-19 pandemic virus highlights the need to fill the vast gaps in our knowledge of SARS-CoV-2 ecology and evolution in non-human hosts. Here, we detected that SARS-CoV-2 was introduced from humans into white-tailed deer more than 30 times in Ohio, USA during November 2021-March 2022. Subsequently, deer-to-deer transmission persisted for 2–8 months, disseminating across hundreds of kilometers. Newly developed Bayesian phylogenetic methods quantified how SARS-CoV-2 evolution is not only three-times faster in white-tailed deer compared to the rate observed in humans but also driven by different mutational biases and selection pressures. The long-term effect of …


Analysis Of Mitochondrial Dna Sequence Data Demonstrates That Monophyly Of Myotis Occultus Is Complicated By Greater Sampling Of Myotis Lucifugus, Jeffrey M. Lorch, Daniel R. Taylor, Antoinette J. Piaggio Dec 2023

Analysis Of Mitochondrial Dna Sequence Data Demonstrates That Monophyly Of Myotis Occultus Is Complicated By Greater Sampling Of Myotis Lucifugus, Jeffrey M. Lorch, Daniel R. Taylor, Antoinette J. Piaggio

United States Department of Agriculture Wildlife Services: Staff Publications

Abstract

The validity of Myotis occultus as a species unique from Myotis lucifugus has been a source of debate. Most recently, many authorities treat M. occultus as a distinct species, at least in part because a previous study showed that M. occultus and M. l. carissima (the subspecies that occurs in closest geographic proximity to M. occultus) form separate monophyletic clades based on sequences of two mitochondrial genes (cytochrome-b [cytb] and cytochrome oxidase subunit II [COII]). We re-evaluated the phylogenetic relationship between M. occultus and M. lucifugus based on mitochondrial sequences …


First Draft Genome Of Thecaphora Frezii, Causal Agent Of Peanut Smut Disease, Renee S. Arias, Cinthia Conforto, Valerie A. Orner, Edgardo J. Carloni, Juan H. Soave, Alicia N. Massa, Marshall C. Lamb, Nelson Bernardi-Lima, Alejandro M. Rago Dec 2023

First Draft Genome Of Thecaphora Frezii, Causal Agent Of Peanut Smut Disease, Renee S. Arias, Cinthia Conforto, Valerie A. Orner, Edgardo J. Carloni, Juan H. Soave, Alicia N. Massa, Marshall C. Lamb, Nelson Bernardi-Lima, Alejandro M. Rago

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Objectives: The fungal pathogen Thecaphora frezii Carranza & Lindquist causes peanut smut, a severe disease currently endemic in Argentina. To study the ecology of T. frezii and to understand the mechanisms of smut resistance in peanut plants, it is crucial to know the genetics of this pathogen. The objective of this work was to isolate the pathogen and generate the first draft genome of T. frezii that will be the basis for analyzing its potential genetic diversity and its interaction with peanut cultivars. Our research group is working to identify peanut germplasm with smut resistance and to understand the genetics …


Predicting Body Weight Of Cattle And Nutrient Digestion Of Individual Sweet Bran Components To Improve Beef Cattle Production Efficiency, Dalton J. Anderson Dec 2023

Predicting Body Weight Of Cattle And Nutrient Digestion Of Individual Sweet Bran Components To Improve Beef Cattle Production Efficiency, Dalton J. Anderson

Department of Animal Science: Dissertations, Theses, and Student Research

Efficiency is of high importance to beef cattle producers to ensure that beef cattle are being raised without unnecessary resource use. Two separate experiments were conducted to evaluate potential strategies to improve efficiency in beef cattle production. A metabolism study was conducted to evaluate digestion parameters of individual Sweet Bran components. The objective was to determine the effects of diet components on nutrient digestion and rumen fermentation parameters. The individual components of Sweet Bran (Corn bran, solvent-extracted germ meal, and corn steep liquor) were included at 40% diet dry matter (DM) in a steam-flaked corn (SFC) based diet. A SFC-based …


Investigation Of Breeding Objectives And Indexes-In-Retrospect, Hunter F. Valasek Dec 2023

Investigation Of Breeding Objectives And Indexes-In-Retrospect, Hunter F. Valasek

Department of Animal Science: Dissertations, Theses, and Student Research

This thesis presents two projects that investigate the impacts of breeding objectives and indexes-in-retrospect within the beef cattle industry. The first project synthesized how changes due to planning horizon, breeding system, and sale endpoint in the economic breeding goal of cattle influence the relative emphasis of traits as well as the re-ranking of selection candidates. A total of three breeding systems that represent straightbred and crossbred cattle were used along with six planning horizons (2, 5, 10, 20, 30, and 50 yrs.) for both a weaning and slaughter point of sale. The combination of these different breeding objective attributes resulted …


The Evaluation Of Feed Additives On Reducing Enteric Methane Production From Cattle, Reba L. Colin Dec 2023

The Evaluation Of Feed Additives On Reducing Enteric Methane Production From Cattle, Reba L. Colin

Department of Animal Science: Dissertations, Theses, and Student Research

Environmental sustainability can be positively impacted by the inclusion of feed additives to reduce enteric methane production from cattle. Methane production can be affected by feed additives that either alter the rumen environment or act as methanogenesis inhibitors. A reduction in methane from cattle can contribute to meeting carbon neutrality.

A metabolism study was conducted to evaluate Alga 1.0, a product containing bromoform, fed to cattle to evaluate the effects on gas emissions. Treatments were (0, 69, or 103 g/d Alga 1.0) fed in a corn-based diet. Headbox-style indirect calorimeters were used to measure gas emissions. Feeding Alga 1.0 linearly …


Forages And Technology Management In Growing And Finishing Beef Cattle Systems, Kelton Cole Adair Dec 2023

Forages And Technology Management In Growing And Finishing Beef Cattle Systems, Kelton Cole Adair

Department of Animal Science: Dissertations, Theses, and Student Research

A systems study evaluated the effects of an implant [25.7 mg estradiol (Compudose; Elanco Animal Health)] at two rates of gain (LOW: 0.45 kg/d and HIGH: 0.9 kg/d) applied during the winter backgrounding phase in drylot and winter grazing systems and its effects on subsequent phases of production. HIGH supplementation with an implant during the winter backgrounding phase in the drylot system yielded the greatest ending body weight (EBW) during the summer backgrounding phase (P = 0.04). LOW supplementation, without an implant, improved summer backgrounding average daily gain (ADG; P = 0.05) while achieving similar carcass adjusted final body …


Performing Motorized-To-Non-Motorized Crash Analysis Using Multi-Model Lbs Traffic Data Calibrated Through Random Forest Models, Nicholas Aldridge Dec 2023

Performing Motorized-To-Non-Motorized Crash Analysis Using Multi-Model Lbs Traffic Data Calibrated Through Random Forest Models, Nicholas Aldridge

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Traditionally, traffic prediction has been an engineering challenge due to insufficient quantities of data. In this thesis, the use of a new type of data called location-based service (LBS) data from StreetLight (StL) and a large database of multi-modal traffic counts are used to infer vehicle, pedestrian, and bicycle volumes on all road segments in Lincoln, Nebraska. Additional land use features are incorporated into the model from the EPA Smart Location Database (EPA-SLD). The use of random forest models provides strong measures of fit for all modes, with R2 scores of 0.98 for both the bicycle and pedestrian modes …


Bridge Deck Overhang Design: Effective Vehicle Impact Loads, Ultimate Strength, And Implications On Bridge Rail Performance, Andrew Eric Loken Dec 2023

Bridge Deck Overhang Design: Effective Vehicle Impact Loads, Ultimate Strength, And Implications On Bridge Rail Performance, Andrew Eric Loken

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

When an errant vehicle strikes a bridge railing on a typical girder bridge, impact loads are transmitted downward through the railing and inward through the deck overhang to the primary superstructure elements. In this research effort, design demands for deck overhangs supporting concrete railings were characterized using physical testing and complementary modeling in LS-DYNA. Physical test and modeling results were also used to better characterize the ultimate strength of overhangs supporting post-and-beam railings. Ultimately, a novel design methodology for deck overhangs was developed and proposed for adoption to the AASHTO LRFD Bridge Design Specifications (BDS).

For overhangs with solid concrete …


An Icfd Fluid Model Used In A Mash Tl-6 Vehicle Model, Md Zunayed Habib Dec 2023

An Icfd Fluid Model Used In A Mash Tl-6 Vehicle Model, Md Zunayed Habib

Department of Mechanical and Materials Engineering: Dissertations, Theses, and Student Research

The objective of this thesis was to develop an ICFD finite element model of a partially filled deformable container suitable for impact scenarios. This model will be later incorporated into the existing TL-6 vehicle model, which is a tractor-tank trailer vehicle model. Previous finite element fluid models for the TL-6 vehicle used an elastic fluid model, which could not predict the fluid behavior correctly.

A study was conducted on the ICFD modeling and an improved ICFD model has been developed using the LS-DYNA, a finite element analysis software. Different properties and parameters of the fluid and the container were adopted …


Characterization Of Interlayer Laser Shock Peening During Fused Filament Fabrication Of Polylactic Acid (Pla), Fabien Denise Dec 2023

Characterization Of Interlayer Laser Shock Peening During Fused Filament Fabrication Of Polylactic Acid (Pla), Fabien Denise

Department of Mechanical and Materials Engineering: Dissertations, Theses, and Student Research

The field of additive manufacturing (AM) has gained a significant amount of popularity due to the increasing need for more sustainable manufacturing techniques and the adaptive development of complex product geometries. The problem is that AM parts routinely exhibit flaws or weaknesses that affect functionality or performance. Over the years, surface treatments have been developed to compensate certain flaws or weaknesses in manufactured products. Combining surface treatments with the modularity of additive manufacturing could lead to more adaptable and creative improvements of product functions in the future. The current work evaluates the feasibility of pursuing a new research axis in …


Experimental Analysis Of Nonlinear Wave Propagation In Bistable Mechanical Metamaterials With A Defect, Samuel R. Harre Dec 2023

Experimental Analysis Of Nonlinear Wave Propagation In Bistable Mechanical Metamaterials With A Defect, Samuel R. Harre

Department of Mechanical and Materials Engineering: Dissertations, Theses, and Student Research

Mechanical metamaterials built up of compliant units can support the propagation of linear and nonlinear waves. A popular architecture consists of a one-dimensional chain of bistable elements connected by linear springs. This type of chain can support nonlinear transition waves that switch each element from one stable state to the other as they propagate along the chain. One way to manipulate the propagation of such waves is via introduction of a local inhomogeneity, i.e., a defect in the otherwise periodic chain. Recent analytical and numerical work has shown that based on its initial velocity, a transition wave may be reflected, …


Capturing The Generation And Structural Transformations Of Molecular Ions, Jun Heo, Doeyeong Kim, Alekos Segalina, Hosung Ki, Doo-Sik Ahn, Seonggon Lee, Jungmin Kim, Yongjun Cha, Kyung Won Lee, Jie Yang, J. Pedro F. Nunes, Xijie Wang, Hyotcherl Ihee Nov 2023

Capturing The Generation And Structural Transformations Of Molecular Ions, Jun Heo, Doeyeong Kim, Alekos Segalina, Hosung Ki, Doo-Sik Ahn, Seonggon Lee, Jungmin Kim, Yongjun Cha, Kyung Won Lee, Jie Yang, J. Pedro F. Nunes, Xijie Wang, Hyotcherl Ihee

Department of Physics and Astronomy: Faculty Publications

Molecular ions are ubiquitous and play pivotal roles1–3 in many reactions, particularly in the context of atmospheric and interstellar chemistry4–6. However, their structures and conformational transitions7,8, particularly in the gas phase, are less explored than those of neutral molecules owing to experimental difficulties. A case in point is the halonium ions9–11, whose highly reactive nature and ring strain make them short-lived intermediates that are readily attacked even by weak nucleophiles and thus challenging to isolate or capture before they undergo further reaction. Here we show that megaelectronvolt ultrafast electron diffraction (MeV-UED)12–14, …


Measurements Of Azimuthal Anisotropy Of Nonprompt D0 Mesons In Pbpb Collisions At √𝑠Nn = 5.02 Tev, A. Tumasyan Nov 2023

Measurements Of Azimuthal Anisotropy Of Nonprompt D0 Mesons In Pbpb Collisions At √𝑠Nn = 5.02 Tev, A. Tumasyan

Department of Civil and Environmental Engineering: Faculty Publications

Measurements of the elliptic (𝑣2) and triangular (𝑣3) azimuthal anisotropy coefficients are presented for D0 mesons produced in b hadron decays (nonprompt D0 mesons) in lead-lead collisions at √ 𝑠NN = 5.02 TeV. The results are compared with previously published charm meson anisotropies measured using prompt D0 mesons. The data were collected with the CMS detector in 2018 with an integrated luminosity of 0.58 nb−1. Azimuthal anisotropy is sensitive to the interactions of quarks with the hot and dense medium created in heavy ion collisions. Comparing results for prompt and …


Disentangling Trend Risk And Basis Risk With Functional Time Series, Yanxin Liu, Johnny Siu-Hang Li Nov 2023

Disentangling Trend Risk And Basis Risk With Functional Time Series, Yanxin Liu, Johnny Siu-Hang Li

Department of Finance: Faculty Publications

In recent multi-population stochastic mortality models, one critical scientific issue is the vague distinction between trend risk and population basis risk. In particular, the cross- and auto-correlations between the innovations of the latent factors representing the common trend and the population-specific trends are often assumed to be non-existent, although they are possibly statistically significant. While it is theoretically possible to capture such correlations by treating the latent factors as a vector time series, the resulting model would contain a large number of parameters, which may in turn lead to robustness problems. In this paper, we address these issues by the …


Do Organic Farming Practices Improve Soil Physical Properties?, Humberto Blanco-Canqui, Sabrina J. Ruis, Charles A. Francis Nov 2023

Do Organic Farming Practices Improve Soil Physical Properties?, Humberto Blanco-Canqui, Sabrina J. Ruis, Charles A. Francis

Department of Agronomy and Horticulture: Faculty Publications

Organic farming (OF) is a reemerging system that could address food security and adverse environmental footprints of conventional farming (CF). However, how OF affects the soil physical environment, an essential pillar for soil ecosystem service delivery, is not well understood. This paper (1) reviews published global literature up to 13 July 2023 regarding the impacts of OF on soil physical properties compared with CF and (2) underlines research needs. Literature indicates OF improves some soil physical properties relative to CF although studies on some properties were few. Specifically, OF increased wet aggregate stability, saturated hydraulic conductivity, and plant available water …


Lactic Acid Bacterial Surface Display Of Scytovirin Inhibitors For Anti-Ebolavirus Infection, Joshua Wiggins, Ngan Nguyen, Wenzhong Wei, Leah Liu Wang, Haley Hollingsead Olson, Shi-Hua Xiang Nov 2023

Lactic Acid Bacterial Surface Display Of Scytovirin Inhibitors For Anti-Ebolavirus Infection, Joshua Wiggins, Ngan Nguyen, Wenzhong Wei, Leah Liu Wang, Haley Hollingsead Olson, Shi-Hua Xiang

Nebraska Center for Virology: Faculty Publications

Scytovirin (SVN) is a lectin from cyanobacteria which has a strong inhibitory activity against Ebola virus infection. We engineered scytovirin as the inhibitor for surface display of lactic acid bacteria to block Ebola virus infection. Two different bacterial strains (Lactobacillus casei and Lactococcus lactis) were successfully engineered for scytovirin expression on the bacterial surface. These bacteria were found to be effective at neutralizing pseudotyped Ebolavirus in a cell-based assay. This approach can be utilized for prophylactic prevention, as well as for treatment. Since lactic acid bacteria can colonize the human body, a long-term efficacy could be achieved. Furthermore, …


Tulathromycin Metaphylaxis Increases Nasopharyngeal Isolation Of Multidrug Resistant Mannheimia Haemolytica In Stocker Heifers, William B. Crosby, Brandi B. Karisch, Lari M. Hiott, Lee J. Pinnell, Alexandra Pittman, Jonathan G. Frye, Charlene R. Jackson, John Dustin Loy, William B. Epperson, John Blanton Jr., Sarah F. Capik, Paul S. Morley, Amelia R. Woolums Nov 2023

Tulathromycin Metaphylaxis Increases Nasopharyngeal Isolation Of Multidrug Resistant Mannheimia Haemolytica In Stocker Heifers, William B. Crosby, Brandi B. Karisch, Lari M. Hiott, Lee J. Pinnell, Alexandra Pittman, Jonathan G. Frye, Charlene R. Jackson, John Dustin Loy, William B. Epperson, John Blanton Jr., Sarah F. Capik, Paul S. Morley, Amelia R. Woolums

School of Veterinary and Biomedical Sciences: Faculty Publications

Bovine respiratory disease (BRD) is a leading cause of disease in feedlot and stocker calves with Mannheimia haemolytica (MH) as one of the most common etiologies. One of the most effective means of controlling BRD is through metaphylaxis, which involves administering antimicrobials to all animals at high risk of developing BRD. However, increasing prevalence of multidrug resistant (MDR) MH may reduce efficacy of metaphylaxis due to decreased susceptibility to drugs used for metaphylaxis. Primarily, this study aimed to determine the effect of tulathromycin metaphylaxis and subsequent BRD treatment on antimicrobial resistance (AMR) in MH isolated from stocker calves. Secondary objectives …


Root-Associated Bacterial Communities And Root Metabolite Composition Are Linked To Nitrogen Use Efficiency In Sorghum, Yen Ning Chai, Yunhui Qi, Emily Goren, Dawn Chiniquy, Amy M. Sheflin,, Susannah G. Tringe, Jessica E. Prenni, Peng Liu, Daniel P. Schachtman Nov 2023

Root-Associated Bacterial Communities And Root Metabolite Composition Are Linked To Nitrogen Use Efficiency In Sorghum, Yen Ning Chai, Yunhui Qi, Emily Goren, Dawn Chiniquy, Amy M. Sheflin,, Susannah G. Tringe, Jessica E. Prenni, Peng Liu, Daniel P. Schachtman

Department of Agronomy and Horticulture: Faculty Publications

The development of cereal crops with high nitrogen use efficiency (NUE) is a priority for worldwide agriculture. In addition to conventional plant breeding and genetic engineering, the use of the plant microbiome offers another approach to improving crop NUE. To gain insight into the bacterial communities associated with sorghum lines that differ in NUE, a field experiment was designed comparing 24 diverse Sorghum bicolor lines under sufficient and deficient nitrogen (N). Amplicon sequencing and untargeted gas chromatography–mass spectrometry were used to characterize the bacterial communities and the root metabolome associated with sorghum genotypes varying in sensitivity to low N. We …


Enzyme Kinetics Of Deoxyuridine Triphosphatase From Western Corn Rootworm, Carlos Riera‑Ruiz, Hideaki Moriyama Nov 2023

Enzyme Kinetics Of Deoxyuridine Triphosphatase From Western Corn Rootworm, Carlos Riera‑Ruiz, Hideaki Moriyama

School of Biological Sciences: Faculty Publications

Objective The Western corn rootworm (WCR), Diabrotica virgifera virgifera, is a highly adaptable insect pest that has evolved resistance to a variety of control strategies, including insecticides. Therefore, it is interesting to examine how housekeeping proteins in WCR have been changed under WCR-controlling strategies. In this study, we focused on one of such proteins in WCR, a ubiquitous enzyme 5’-triphosphate nucleotidohydrolase (dUTPase). In the thymidine synthetic pathway, dUTPase hydrolyzes deoxyuridine triphosphate (dUTP) and supplies the substrate, deoxyuridine monophosphate, for the thymidylate synthase (TS). It decreases the cellular content of uracil, reducing uracil misincorporation into DNA. Suppressing the dUTPase activity, …


University Of Nebraska-Lincoln Agricultural Research Division 121st Annual Report. July 1, 2006 To June 30, 2007., Institute Of Agriculture And Natural Resources Nov 2023

University Of Nebraska-Lincoln Agricultural Research Division 121st Annual Report. July 1, 2006 To June 30, 2007., Institute Of Agriculture And Natural Resources

Agricultural Research Division: News and Annual Reports

Our Mission..... 4

Foreword..... 5

Research Highlights..... 6

Faculty Awards and Recognitions....14

Graduate Student Awards and Recognitions...17

Undergraduate Honors Student Research Program...22

Variety and Germplasm Releases....23

Patents.....24

Administration.....25

Administrative Personnel....25

Organizational Chart....26

Administrative Units....27

IANR Research Facilities....28

Faculty.....29

Agricultural/Natural Resources Units....30

Education and Human Sciences Departments...39

Off-Campus Research Centers....40

Interdisciplinary Activities....41

Visiting Scientists/Research Associates....42

Research Projects.....47

Agricultural/Natural Resources Units....47

Education and Human Sciences Departments...52

Off-Campus Research Centers....52

Interdisciplinary Activities....53

Publications.....55

Agricultural/Natural Resources Units....60

Education and Human Sciences Departments...82

Off-Campus Research Centers....85

Research Expenditures....88


Analyzing Major Elements Of Crash Injury Severity Involving Priority-I Detriments Of Vision-Zero Plan, Muhammad Umer Farooq, Cole Madson, Aemal Khattak Nov 2023

Analyzing Major Elements Of Crash Injury Severity Involving Priority-I Detriments Of Vision-Zero Plan, Muhammad Umer Farooq, Cole Madson, Aemal Khattak

Mid-America Transportation Center: Final Reports and Technical Briefs

Road traffic crashes result in significant economic losses for individuals, their families, and entire nations. These losses stem from the expenses associated with injury treatment, as well as the productivity lost due to fatalities or disabilities caused by these injuries. The 2030 Agenda for Sustainable Development set an ambitious target of halving the global number of fatalities and injuries from road traffic crashes by 2020 and achieving zero deaths by 2030, commonly referred to as the 'Target Zero Plan.' The Target Zero Plan prioritizes traffic safety issues into three distinct levels. The three priority levels are determined based on the …


Exploring Statistical And Machine Learning-Based Missing Data Imputation Methods To Improve Crash Frequency Prediction Models For Highway-Rail Grade Crossings, Muhammad Umer Farooq, Aemal Khattak Nov 2023

Exploring Statistical And Machine Learning-Based Missing Data Imputation Methods To Improve Crash Frequency Prediction Models For Highway-Rail Grade Crossings, Muhammad Umer Farooq, Aemal Khattak

Mid-America Transportation Center: Final Reports and Technical Briefs

Highway-rail grade crossings (HRGCs) are critical spatial locations of transportation safety because crashes at HRGCs are often catastrophic, potentially causing several injuries and fatalities. Every year in the United States, a significant number of crashes occur at these crossings, prompting local and state organizations to engage in safety analysis and estimate crash frequency prediction models for resource allocation. These models provide valuable insights into safety and risk mitigation strategies for HRGCs. Furthermore, the estimation of these models is based on inventory details of HRGCs, and their quality is crucial for reliable crash predictions. However, many of these models exclude crossings …


Raccoon Spatial Ecology In The Rural Southeastern United States, Jacob E. Hill, Madison L. Miller, James L. Helton, Richard B. Chipman, Amy Gilbert, University Of Georgia, Guha Dharmarajan, Olin E. Rhodes Jr. Nov 2023

Raccoon Spatial Ecology In The Rural Southeastern United States, Jacob E. Hill, Madison L. Miller, James L. Helton, Richard B. Chipman, Amy Gilbert, University Of Georgia, Guha Dharmarajan, Olin E. Rhodes Jr.

United States Department of Agriculture Wildlife Services: Staff Publications

The movement ecology of raccoons varies widely across habitats with important implications for the management of zoonotic diseases such as rabies. However, the spatial ecology of raccoons remains poorly understood in many regions of the United States, particularly in the southeast. To better understand the spatial ecology of raccoons in the southeastern United States, we investigated the role of sex, season, and habitat on monthly raccoon home range and core area sizes in three common rural habitats (bottomland hardwood, upland pine, and riparian forest) in South Carolina, USA. From 2018–2022, we obtained 264 monthly home ranges from 46 raccoons. Mean …


Raccoon Spatial Ecology In The Rural Southeastern United States, Jacob E. Hill, Madison L. Miller, James L. Helton, Richard B. Chipman, Amy T. Gilbert, James C. Beasley, Guha Dharmarajan, Olin E. Rhodes Jr. Nov 2023

Raccoon Spatial Ecology In The Rural Southeastern United States, Jacob E. Hill, Madison L. Miller, James L. Helton, Richard B. Chipman, Amy T. Gilbert, James C. Beasley, Guha Dharmarajan, Olin E. Rhodes Jr.

United States Department of Agriculture Wildlife Services: Staff Publications

The movement ecology of raccoons varies widely across habitats with important implications for the management of zoonotic diseases such as rabies. However, the spatial ecology of raccoons remains poorly understood in many regions of the United States, particularly in the southeast. To better understand the spatial ecology of raccoons in the southeastern US, we investigated the role of sex, season, and habitat on monthly raccoon home range and core area sizes in three common rural habitats (bottomland hardwood, upland pine, and riparian forest) in South Carolina, USA. From 2018–2022, we obtained 264 monthly home ranges from 46 raccoons. Mean monthly …