Digital Commons Network^™

Communication In The Science-Policy Interface: Evidence From A Boundary Organization In Nebraska, Usa, Sechindra Vallury, Brian C. Chaffin, Samantha L. Hamlin, Craig R. Allen

School of Natural Resources: Faculty Publications

Boundary organizations have a crucial function in environmental governance by facilitating the processes through which scientists and decision-makers generate, exchange, evaluate, and utilize knowledge to identify societal problems, propose potential solutions, and make decisions on appropriate courses of action. This support for evidence-informed decision making is essential in addressing environmental challenges effectively. Despite the growing popularity of boundary organizations, there remains a significant challenge in designing information dissemination platforms to bridge the communication divide between scientific experts and non-experts. To address this gap, we used natural language processing tools to analyze the communication strategies of a specific boundary organization — …

Nebline, August/September 2023

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Understanding the “WHY” of Challenging Behavior in Young Children Can Help With Strategies by Hayley Jackson

5 Reasons to Support Your Farmers Market by Tara Dunker

Recipes of the Month: Chocolate Zucchini Muffins and Fresh Salsa by Kayla Colgrove

Time for Fall Lawn Seeding by Sarah Browning

Garden Guide: Things to Do This Month

Using Water Resources More Efficiently by Becky Schuerman

Parasitoids: Nature’s Biocontrol by Kait Chapman

Lancaster County Super Fair, Aug. 3-12, 2023

Webinars Addressing Biting Pests in Early Childhood and School Settings

Ag Landlord/Tenant Workshop

4-H Announcements for 4-H'ers or 4-H Volunteers

Heart of 4-H Volunteer Award: …

A Manual For The Recovery Of Cbrne (Chemical, Biological, Radiological, Nuclear, And Explosive) Contaminated Human Remains, Patrick Barchett

Department of Anthropology: Theses and Student Research

Despite many international agreements and treaties prohibiting the use of CBRNE (Chemical, Biological, Radiological, Nuclear, and Explosive) weaponry, the proliferation of contaminated human remains displays the historic and continued use of inhumane war efforts. Due to the complications of recovery, contaminated human remains are often left unrecovered and unidentified. This thesis works to create a framework for the recovery of contaminated human remains for organizations with a focus on creating safe and sustainable working environments for those conducting the recovery. Much of this thesis assumes the remains are being recovered from interred mass graves. This thesis provides an introduction to …

Seek And Classify: End-To-End Joint Multi-Signal Detection And Classification Using Deep Learning, Prashant Subedi

School of Computing: Dissertations, Theses, and Student Research

The rise in the use of wireless communication has led to the problem of spectrum scarcity in licensed bands. The popularity of Internet of Things (IoT) requires innovative solutions that maximize the use of available spectrum to support the increasing number of connected devices. This thesis tackles two significant problems in wireless communication: the need for efficient spectrum sensing techniques and the scarcity of large, diverse raw in-phase (I) and quadrature (Q) datasets.

The ability to detect and classify modulation of the signals efficiently can enable a cognitive radio to monitor the spectrum activity in real time and utilize unused …

Influence Of Overcooking On Food Digestibility And In Vitro Fermentation, Wensheng Ding

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Areas of char or overcooking commonly appear in foods people consume. It has been reported that overcooked food is harmful to human health. However, little research exists on the effect of overcooking on in vitro protein and starch digestibility and gut microbial fermentation. This study aimed to reveal the connection between overcooking and in vitro protein and starch digestibility and gut microbial fermentation. In vitro protein digestibility of an overcooked ground beef patty was almost half that of a standard cooked sample (27 ± 2% versus 48 ± 6%, respectively; p = 0.02). Whole wheat bread protein digestibility was also …

Services Provided To Aging Individuals With Intellectual Disabilities: Survey Of Speech Language Pathologists, Claire H. Gatewood

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Purpose: The present study explored current speech-language pathology service provision for aging individuals with intellectual disabilities in the United States, including areas and domains of services, factors restricting service provision, and reported knowledge and confidence of individuals within the field of speech-language pathology in providing services, to identify possible gaps of service and needed improvement.

Methods: Participants (n = 272) from across the United States completed an online survey to gather descriptive information about current speech-language pathology service provision for aging individuals with intellectual disabilities. Participants were recruited through state speech-language hearing associations and universities with speech-language pathology clinics …

Understanding The Factors Affecting Nebraskan Farmers’ And Landowners' Decision To Adopt Precision Agricultural Technologies And Programs, Corrin C. Winter

School of Natural Resources: Dissertations, Theses, and Student Research

Advisors: Andrew Little, Christopher Chizinski

To gain insights into the challenges faced by Nebraska farmers and landowners when adopting precision agriculture technologies, I analyzed data collected from a 2022 survey involving 7,503 participants, consisting of producers and farmland owners from across the state of Nebraska. The primary objective was to provide valuable insights for agencies seeking to improve their precision agriculture and conservation outreach efforts for the benefit of conservation and Nebraskan farmers and landowners. This study aimed to understand key factors influencing adoption behaviors by evaluating the constraints to precision agriculture adoption, assessing the impact of producer and landowner …

Adverse Childhood Experiences Among Adolescent Girls In Residential Treatment: Relationship With Trauma Symptoms, Substance Use, And Delinquency, Akemi E. Mii

Department of Psychology: Dissertations, Theses, and Student Research

Adverse childhood experiences (ACEs) refer to negative events during childhood or adolescence including abuse, maltreatment, and exposure to household dysfunction (Kalmakis & Chandler, 2014). ACEs are associated with negative outcomes including mental and behavioral health concerns and offending (Fox et al., 2015). The risk of negative outcomes associated with ACEs increases when an individual experiences polyvictimization (experiencing multiple types of adverse events; Felitti et al., 1998; Finkelhor et al., 2011). A majority of adolescents served by residential treatment programs (RTPs) have experienced polyvictimization (Briggs et al., 2013). Research examining juvenile offending and youth delinquency has focused on boys. Thus, research …

Positioning Undergraduate Learning Assistants In Instruction: A Case Study Of The La Role In Active Learning Mathematics Classrooms At The University Of Nebraska–Lincoln, Rachel Funk

Department of Mathematics: Dissertations, Theses, and Student Research

Research suggests learning assistant (LA) programs can be a change lever to support the institutionalization of active learning in postsecondary education. Some research suggests LAs offer unique benefits for STEM courses, independent from other change levers, but more research needs to be done to understand how LAs support active learning classrooms, specifically in mathematics. Research on mathematics instruction and the use of reform resources suggests that the successful implementation of reforms is impacted by perceptions individuals hold about that resource, such as the LA role. Yet, there is little research about the LA role in mathematics, particularly where the instructor …

Wild Bee Utilization Of Engineered Pollinator Habitat In Eastern Nebraska Agroecosystems, Shianne Lindsay

Department of Entomology: Dissertations, Theses, and Student Research

Habitat loss is a leading cause of biodiversity decline globally. Further, loss of natural and semi-natural habitat is a major factor contributing to declines in wild pollinator populations. Among intensive agroecosystems in the Midwestern United States, corn, soy, and small grain fields dominate the landscape and leave limited space available for forb-rich habitat that supports diverse pollinator assemblages to exist and thrive. There are numerous barriers and challenges to integrating pollinator habitat into such agroecosystems and quantitative assessments of impacts on pollinator communities are largely lacking. The establishment of high-quality pollinator habitat can provide both high flower abundance and species …

Data-Driven Framework For Predicting And Scheduling Household Charging Of Evs, Ahmad Almaghrebi

Durham School of Architectural Engineering and Construction: Dissertations, Theses, and Student Research

The increasing prevalence of EV charging poses challenges for power grid stability and quality due to high charging load demands. Without effective energy management strategies for EV charging, the simultaneous power demand from numerous EVs can strain the electric grid, impacting power quality and the wholesale electricity market. To address these challenges, this dissertation presents a comprehensive framework comprising five critical tasks: analyzing EV charging behavior, optimizing charging schedules, developing predictive models, analyzing aggregated impacts, and evaluating implications of predicted user behavior on scheduling. By examining EV charging behavior at household and public charging stations, this study aims to understand …

Framework Development For Evaluating The Impacts Of Nursing Home Acoustics On Older Adults, Brian J. Puckett

Durham School of Architectural Engineering and Construction: Dissertations, Theses, and Student Research

Nursing homes are complex, multi-faceted environments which provide necessary medical supervision and housing for older adults in need of long-term care. With cognitive and sensory impairments being common adversities faced throughout the aging process, research is needed to better understand characteristics of the built environment which may contribute to difficulties for nursing home residents. Noise, in particular, has been shown to be problematic for nursing home residents in a variety of ways including disrupting sleep, associating with behavioral and psychological symptoms of dementia, and leading to increased readmission to hospitals due to verbal communication misunderstandings. As the older adult population …

Drinking Water Quality Management Progress In Ontario, Two Decades After Walkerton, Megan Fuller, Emma Wells, Laith Furatian, Ian Douglas, Kaycie Lane

Department of Civil and Environmental Engineering: Faculty Publications

Following the waterborne disease outbreak in Walkerton, Ontario, the province made significant efforts to implement recommendations of the public inquiry that resulted. As Ontario reformed its drinking water sector, other jurisdictions were advancing risk-based quality management frameworks for drinking water, including the World Health Organization (WHO) through its water safety plan (WSP) framework. Two decades after the Walkerton tragedy, this paper seeks to: (i) evaluate alignment of Ontario’s Drinking Water Quality Management Standard (DWQMS) with the WSP framework (ii) review readily available data for evidence that Ontario’s DWQMS implementation has improved drinking water safety and promoted a preventive approach through …

Differential Membrane Binding Of Α/Β-Peptide Foldamers: Implications For Cellular Delivery And Mitochondrial Targeting, Tzong-Hsien Lee, James W. Checco, Tess Malcolm, Chelcie H. Eller, Ronald T. Raines, Samuel H. Gellman, Erinna F. Lee, W. Douglas Fairlie, Marie-Isabel Aguilar

Department of Chemistry: Faculty Publications

The intrinsic pathway of apoptosis is regulated by the Bcl-2 family of proteins. Inhibition of the anti-apoptotic members represents a strategy to induce apoptotic cell death in cancer cells. We have measured the membrane binding properties of a series of peptides, including modified α/β-peptides, designed to exhibit enhanced membrane permeability to allow cell entry and improved access for engagement of Bcl-2 family members. The peptide cargo is based on the pro-apoptotic protein Bim, which interacts with all anti-apoptotic proteins to initiate apoptosis. The α/β-peptides contained cyclic β-amino acid residues designed to increase their stability and membrane-permeability. Dual polarisation interferometry was …

Loss Of Flockmates Weakens Winter Site Fidelity In Golden-Crowned Sparrows (Zonotrichia Atricapilla), Anastasia E. Madsen, Bruce E. Lyon, Alexis S. Chaine, Theadora A. Block, Daizaburo Shizuka

School of Biological Sciences: Faculty Publications

Animal social interactions have an intrinsic spatial basis as many of these interactions occur in spatial proximity. This presents a dilemma when determining causality: Do individuals interact socially because they happen to share space, or do they share space because they are socially linked? We present a method that uses demographic turnover events as a natural experiment to investigate the links between social associations and space use in the context of interannual winter site fidelity in a migratory bird. We previously found that golden-crowned sparrows (Zonotrichia atricapilla) show consistent flocking relationships across years, and that familiarity between individuals …

Public Mediations Of Accountability In The #Metoo Era, Amanda Brand

Department of Communication Studies: Dissertations, Theses, and Student Research

Tarana Burke initially launched the Me Too movement to cultivate solidarity among sexual assault survivors in 2006, and public appropriations of this effort have resulted in a kairotic moment of accountability in sexual assault cases. Particularly, the 2017 hashtag, #MeToo populates media platforms as the public invokes it to make sense of sexual assault cases, bearing witness to victim-survivors, assigning blame, or disavowing culpability. Challenging legacies of public denial, #MeToo marks a cultural shift in which victim-survivors are not only speaking out, they are also being heard and believed. I argue that accountability is rhetorically-constructed, negotiated, and imposed through …

A Synopsis Of The New World Genera Of Phileurini (Coleoptera: Scarabaeidae: Dynastinae), With English And Spanish Keys To The Genera, Brett C. Ratcliffe, Ronald D. Cave

Department of Entomology: Faculty Publications

The 22 genera of New World Phileurini are briefly reviewed and characterized, and English and Spanish keys to all the genera are provided for the first time, thus facilitating generic identifications. Chiliphileurus Endrödi (a junior synonym of the Asian genus Eophileurus Arrow), Moraguesia Dechambre (transferred to Oxyligyrus Arrow in the tribe Pentodontini), and Platyphileurus Ohaus (transferred to the tribe Oryctini) are no longer part of the New World phileurine fauna. The monobasic Phileucourtus Dechambre is considered a junior synonym of Hemiphileurus Kolbe.

Nebraska Extension: Supporting The Well-Being Of Farmers And Ranchers, Jessica Groskopf

Cornhusker Economics

Nebraska Extension is a proud member of the North Central Farm and Ranch Stress Assistance Center. A regional center funded by USDA NIFA, it delivers farm and ranch stress and mental health resources and services in 12 states, including Nebraska.

Since 2021, several programs delivered by Nebraska Extension and its partners have been supported with these funds. This article will review the key programs supported in Nebraska.

Analysis Of Syndrome-Based Iterative Decoder Failure Of Qldpc Codes, Kirsten D. Morris, Tefjol Pllaha, Christine A. Kelley

Department of Mathematics: Faculty Publications

Iterative decoder failures of quantum low density parity check (QLDPC) codes are attributed to substructures in the code’s graph, known as trapping sets, as well as degenerate errors that can arise in quantum codes. Failure inducing sets are subsets of codeword coordinates that, when initially in error, lead to decoding failure in a trapping set. In this paper we examine the failure inducing sets of QLDPC codes under syndrome-based iterative decoding, and their connection to absorbing sets in classical LDPC codes.

Signal Classification Based On Analog Computing Using Mems Network, Mohammad Okour

Durham School of Architectural Engineering and Construction: Dissertations, Theses, and Student Research

The rising complexity of machine learning algorithms and Artificial Intelligence in many applications, such as smart building, has prompted the development of alternate computing options. Because of their compact size, low power consumption, and diverse functionality, microelectromechanical systems (MEMS) have emerged as a possible candidate. This thesis focuses on using MEMS networks as computing units to classify a simple signal classification task using neural network methodology. The study intends to show the potential of using MEMS as an analog computing unit by discussing the advantage of the bi-stability pull-in behavior and hysteresis to create an accurate classifier of these waveforms. …

Twin-Solute, Twin-Dislocation And Twin-Twin Interactions In Magnesium, Materials Yue, Jian Wang, Jian-Feng Nie

Department of Mechanical and Materials Engineering: Faculty Publications

Magnesium alloys have received considerable research interest due to their lightweight, high specific strength and excellent castability. However, their plastic deformation is more complicated compared to cubic materials, primarily because their low-symmetry hexagonal closepacked (hcp) crystal structure. Deformation twinning is a crucial plastic deformation mechanism in magnesium, and twins can affect the evolution of microstructure by interacting with other lattice defects, thereby affecting the mechanical properties. This paper provides a review of the interactions between deformation twins and lattice defects, such as solute atoms, dislocations and twins, in magnesium and its alloys. This review starts with interactions between twin boundaries …

Waterbody Size Predicts Bank- And Boat-Angler Efforts, Derek S. Kane, Kevin L. Pope, Keith D. Koupal, Mark A. Pegg, Christopher J. Chizinski, Mark A. Kaemingk

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Bank- and boat-angler efforts are logistically difficult and costly to estimate, preventing landscape-scale estimates that are required to address current and future challenges (e.g., climate change, invasive species) for inland recreational fisheries. Using a large Nebraska, USA, recreational fishery dataset (N = 67 waterbodies), we demonstrate that waterbody size can be used to predict bank- and boat-angler efforts across a heterogeneous landscape of extra small (< 104 ha) and large (> 647 ha) waterbodies. Bank and boat anglers respond to waterbody size, however these relationships appear to be unique between the two angler types. Boat-angler efforts increased as a function of waterbody size, whereas bank-angler …

An Ai-Based Intervention For Improving Undergraduate Stem Learning, Mohammad Rashedul Hasan, Bilal Khan

Department of Electrical and Computer Engineering: Faculty Publications

We present results from a small-scale randomized controlled trial that evaluates the impact of just-in-time interventions on the academic outcomes of N = 65 undergraduate students in a STEM course. Intervention messaging content was based on machine learning forecasting models of data collected from 537 students in the same course over the preceding 3 years. Trial results show that the intervention produced a statistically significant increase in the proportion of students that achieved a passing grade. The outcomes point to the potential and promise of just-in-time interventions for STEM learning and the need for larger fully-powered randomized controlled trials.

Chloroquine And Cytosolic Galectins Affect Endosomal Escape Of Antisense Oligonucleotides After Stabilin-Mediated Endocytosis, Ekta Pandey, Ed Harris

Department of Biochemistry: Faculty Publications

Non-DNA-binding Stabilin-2/HARE receptors expressed on liver sinusoidal endothelial cells specifically bind to and internalize several classes of phosphorothioate antisense oligonucleotides (PS-ASOs). After Stabilin-mediated uptake, PS-ASOs are trafficked within endosomes (>97%–99%), ultimately resulting in destruction in the lysosome. The ASO entrapment in endosomes lowers therapeutic efficacy, thereby increasing the overall dose for patients. Here, we use confocal microscopy to characterize the intracellular route transverse by PS-ASOs after Stabilin receptor-mediated uptake in stable recombinant Stabilin-1 and -2 cell lines. We found that PS-ASOs as well as the Stabilin-2 receptor transverse the classic path: clathrincoated vesicle-early endosome-late endosome-lysosome. Chloroquine exposure facilitated endosomal …

Agricultural Research Service Weed Science Research: Past, Present, And Future, Stephen L. Young, James V. Anderson, Scott R. Baerson, Joanna Bajsa-Hirschel, Dana M. Blumenthal, Chad S. Boyd, Clyde D. Boyette, Eric B. Brennan, Charles L. Cantrell, Wun S. Chao, Joanne C. Chee-Sanford, Charlie D. Clements, F. Allen Dray, Stephen O. Duke, Kayla M. Eason, Reginald S. Fletcher, Michael R. Fulcher, Brenda J. Grewell, Erik P. Hamerlynck, Robert E. Hoagland, David P. Horvath, Eugene P. Law, Daniel E. Martin, Clint Mattox, Steven B. Mirsky, Patrick J. Moran, Rebecca C. Mueller, Vijay K. Nandula, Beth A. Newingham, Zhiqiang Pan, Lauren M. Porensky, Paul D. Pratt, Andrew J. Price, Brian G. Rector, Krishna N. Reddy, Roger L. Sheley, Lincoln Smith, Melissa C. Smith, Keirith A. Snyder, Matthew A. Tancos

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS) has been a leader in weed science research covering topics ranging from the development and use of integrated weed management (IWM) tactics to basic mechanistic studies, including biotic resistance of desirable plant communities and herbicide resistance. ARS weed scientists have worked in agricultural and natural ecosystems, including agronomic and horticultural crops, pastures, forests, wild lands, aquatic habitats, wetlands, and riparian areas. Through strong partnerships with academia, state agencies, private industry, and numerous federal programs, ARS weed scientists have made contributions to discoveries in the newest fields of robotics and genetics, as well …

Collaborative Decision Making Improves Interpersonal Psychotherapy Efficiency: A Randomized Clinical Trial With Postpartum Women, Scott Stuart, Rebecca L. Brock, Erin Ramsdell, Stephan Arndt, Michael W. O'Hara

Department of Psychology: Faculty Publications

Background: Randomized controlled trials of Interpersonal Psychotherapy (IPT) and other psychotherapies for depression have required strict adherence to protocol and do not allow for clinical judgment in deciding frequency of sessions. To determine if such protocols were more effective than allowing therapists to use their clinical judgment, we compared “Clinician- Managed” IPT (CM-IPT), in which clinicians and patients with postpartum depression were allotted 12 sessions and determined collaboratively when to use them, to a once weekly 12 session protocol (“Standard IPT”). We hypothesized that CM-IPT would be more efficient, requiring fewer sessions to reach an equivalent acute outcome, and …

A Protein Kinase Coordinates Cycles Of Autophagy And Glutaminolysis In Invasive Hyphae Of The Fungus Magnaporthe Oryzae Within Rice Cells, Gang Li, Ziwen Gong, Nawaraj Dulal, Margarita Marroquin-Guzman, Raquel O. Rocha, Michael Richter, Richard A. Wilson

Department of Plant Pathology: Faculty Publications

The blast fungus Magnaporthe oryzae produces invasive hyphae in living rice cells during early infection, separated from the host cytoplasm by plantderived interfacial membranes. However, the mechanisms underpinning this intracellular biotrophic growth phase are poorly understood. Here, we show that the M. oryzae serine/threonine protein kinase Rim15 promotes biotrophic growth by coordinating cycles of autophagy and glutaminolysis in invasive hyphae. Alongside inducing autophagy, Rim15 phosphorylates NADdependent glutamate dehydrogenase, resulting in increased levels of α- ketoglutarate that reactivate target-of-rapamycin (TOR) kinase signaling, which inhibits autophagy. Deleting RIM15 attenuates invasive hyphal growth and triggers plant immunity; exogenous addition of α-ketoglutarate prevents these …

A Protein Kinase Coordinates Cycles Of Autophagy And Glutaminolysis In Invasive Hyphae Of The Fungus Magnaporthe Oryzae Within Rice Cells, Gang Li, Ziwen Gong, Nawaraj Dulal, Margarita Marroquin-Guzman, Raquel O. Rocha, Michael Richter, Richard Wilson

Department of Plant Pathology: Faculty Publications

The blast fungus Magnaporthe oryzae produces invasive hyphae in living rice cells during early infection, separated from the host cytoplasm by plantderived interfacial membranes. However, the mechanisms underpinning this intracellular biotrophic growth phase are poorly understood. Here, we show that the M. oryzae serine/threonine protein kinase Rim15 promotes biotrophic growth by coordinating cycles of autophagy and glutaminolysis in invasive hyphae. Alongside inducing autophagy, Rim15 phosphorylates NADdependent glutamate dehydrogenase, resulting in increased levels of α- ketoglutarate that reactivate target-of-rapamycin (TOR) kinase signaling, which inhibits autophagy. Deleting RIM15 attenuates invasive hyphal growth and triggers plant immunity; exogenous addition of α-ketoglutarate prevents these …

A Protein Kinase Coordinates Cycles Of Autophagy And Glutaminolysis In Invasive Hyphae Of The Fungus Magnaporthe Oryzae Within Rice Cells, Gang Li, Ziwen Gong, Nawaraj Dulal, Margarita Marroquin-Guzman, Raquel O. Rocha, Michael Richter, Richard Wilson

Department of Plant Pathology: Faculty Publications

The blast fungus Magnaporthe oryzae produces invasive hyphae in living rice cells during early infection, separated from the host cytoplasm by plantderived interfacial membranes. However, the mechanisms underpinning this intracellular biotrophic growth phase are poorly understood. Here, we show that the M. oryzae serine/threonine protein kinase Rim15 promotes biotrophic growth by coordinating cycles of autophagy and glutaminolysis in invasive hyphae. Alongside inducing autophagy, Rim15 phosphorylates NADdependent glutamate dehydrogenase, resulting in increased levels of α- ketoglutarate that reactivate target-of-rapamycin (TOR) kinase signaling, which inhibits autophagy. Deleting RIM15 attenuates invasive hyphal growth and triggers plant immunity; exogenous addition of α-ketoglutarate prevents these …

Evaluating Salmonella Cross Contamination In Raw Chicken Thighs In Simulated Post-Chill Tanks, Raziya Sadat

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Salmonella contamination poses a significant risk to food safety, particularly in poultry products and public health. This study aims to investigate the effectiveness of peracetic acid (PAA) treatment in mitigating Salmonella cross-contamination in chicken thighs within simulated post-chill tanks, considering different inoculum concentrations and organic matter (OM) levels.

The experiment involved the use of five distinct Salmonella serovars to inoculate chicken thighs, which were categorized into two groups: higher inoculation level (ca. 7 log CFU/mL) and lower inoculation level (ca, 4 log CFU/mL). These groups underwent treatment with four different solutions, including 540 ppm PAA with 1.5% OM, 540 ppm …