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Experience Improves The Reliability Of Subjective Measurements Of Temperament In Beef Cattle, Jaime T. Praham, Amy E. Tanner, Mark L. Wahlberg Wahlberg, W. S. Swecker Jr., Ronald M. Lewis

Nebraska Beef Cattle Reports

Reliability of experienced and inexperienced observers when assessing the behavior of cattle when restrained in a squeeze chute (chute score), and when exiting the chute (exit score), was compared. Overall, experienced observers had higher reliability than inexperienced observers. Increasing the number of individuals scoring an animal decreased the degree of agreement. However, within an acceptable tolerance for difference in scores, such disagreement may be beneficial; it allows for subtlety in interpretations of temperament, which when averaged, may better reflect docility. Reliabilities were higher for exit score than chute score. This may reflect the complexity of the trait being evaluated, with …

Antimicrobial Interventions And Application Time Effects On Ground Beef Quality, Ashley R. Mccoy, Dennis E. Burson, Gary A. Sullivan Sullivan

Nebraska Beef Cattle Reports

Small business meat processors can use organic acid antimicrobial interventions to control Shiga toxin-producing E. coli (STEC) when producing ground beef; however, many small producers are concerned about the impact on ground beef quality. The effects of two commonly used organic acids, lactic acid and peroxyacetic acid, were evaluated at short (15 seconds) or extended (3 minutes) raw material dip times on ground beef quality parameters. Beef trim dipped in lactic acid for 3 minutes had a reduction in total aerobic bacteria plate count, but also increased ground beef discoloration and lipid oxidation during retail display. Use of a shorter …

Steer Performance Grazing Corn Residue And Supplemented With Modified Distillers Grains Plus Solubles With Or Without Urea, Robert M. Jones, Robby G. Bondurant, F. Henry Hilscher Hilscher, Jim C. Macdonald

Nebraska Beef Cattle Reports

A growing study was conducted to evaluate the effects of supplementing growing calves grazing corn residue with modified distillers grains plus solubles (MDGS; 3 or 5 lb/d) and with or without urea on growth performance. There were no significant MDGS × urea inclusion interactions observed. Urea inclusion level (0 and 0.12 lb/d) did not affect supplemental intake, ADG, or ending BW. Steers fed 5 lb of MDGS had an increased ADG and a heavier ending BW compared to steers fed 3 lb MDGS daily. Supplemental urea is not necessary when supplementing at least 3 lb MDGS to steers grazing corn …

Evaluation Of Corn Distillers Solubles On Growing Steer Performance, Benjamin H. Hansen, Robert G. Bondurant, F. Henry Hilscher Hilscher, Galen E. Erickson, Jim C. Macdonald

Nebraska Beef Cattle Reports

A growing study evaluated increasing inclusions of corn distillers solubles (CDS) at 10, 20, 30, and 40%, or increasing wet distillers grains plus solubles (WDGS) at 10, 20, 30, and 40% compared to a corn control. Corn was replaced by CDS or WDGS in each forage-based diet. Increasing CDS resulted in a quadratic increase for both DMI and F:G. Increasing WDGS linearly increased both DMI and ADG with no effect on F:G. The energy value of CDS is less than that of corn, whereas WDGS had an energy value similar to corn in growing diets with 50% brome hay. A …

Analyses Of Birthdate And Growth In Beef Heifers Categorized By Puberty And Pregnancy Status, Aline Gomes Da Silva, Andy Roberts, T. L. Meyers, Richard N. Funston

Nebraska Beef Cattle Reports

Heifer records were retrospectively evaluated to see if Julian birthdate, cycling status prior to breeding, and body weight collected from weaning through final pregnancy diagnosis differed when heifers were categorized by 5 different approaches: 1) pubertal status prior to estrous synchronization, 2) whether or not detected in estrus at AI, 3) heifers impregnated by AI vs all other heifers, 4) final pregnancy status, and 5) a 5-way classification accounting for AI and pregnancy status (AI pregnant, heifers subjected to AI that subsequently conceived to bull, heifers not AI that were impregnated by bull, heifers subjected to AI that were not …

The Effect Of Harvest Method And Ammoniation Of Corn Residue On Growing Calf Performance, Ashley C. Conway, Robert G. Bondurant, F. Henry Hilscher Hilscher, James C. Macdonald, Terry J. Klopfenstein, Mary Drewnoski

Nebraska Beef Cattle Reports

A growing trial was conducted to determine the effect of feeding baled corn residue harvested using three different methods, with and without ammoniation of the residue. Residue harvested with the New Holland Cornrower™ with two rows of chopped stem added to the windrow resulted in a 9% increase in ADG compared to conventional rake and bale harvest or turning off the combine spreader and baling tailing. Ammoniation of residue increased ADG 67% (increase of 1.1 lb/d) over non-ammoniated residue. Feed efficiency was not affected by harvest method, but ammoniation decreased F:G by 13% compared to cattle fed non-ammoniated residue. Although …

Impact Of Corn Oil Removal From Modified Distillers Grains Plus Solubles And Supplemental Corn Oil On Finishing Cattle Performance, Jordan E. Burhoop, Curtis J. Bittner, F. Henry Hilscher Hilscher, Matt K. Luebbe, Jim C. Macdonald, Galen E. Erickson

Nebraska Beef Cattle Reports

A finishing study was conducted to evaluate removal of corn oil from modified distillers grains plus solubles (MDGS) and replacement of supplemental corn oil on finishing cattle performance. Four treatments were evaluated: a corn control diet, 40% de-oiled MDGS, 38% de-oiled MDGS plus 2% corn oil to equal the fat content of full fat MDGS, or 40% full fat MDGS. There was a significant improvement in ADG and F:G for cattle fed de-oiled MDGS plus oil compared to other treatments. Cattle fed full fat MDGS had numerically lower ADG and numerically poorer F:G (3.7%) compared with cattle fed MDGS plus …

Genomic Selection In Preliminary Yield Trials In A Winter Wheat Breeding Program, Vikas Belamkar, Mary J. Guttieri, Waseem Hussain, Diego Jarquin, Ibrahim El-Basyoni, Jesse Poland, Aaron J. Lorenz, P. Stephen Baenziger

Department of Agronomy and Horticulture: Faculty Publications

Genomic prediction (GP) is now routinely performed in crop plants to predict unobserved phenotypes. The use of predicted phenotypes to make selections is an active area of research. Here, we evaluate GP for predicting grain yield and compare genomic and phenotypic selection by tracking lines advanced. We examined four independent nurseries of F_3:6 and F_3:7 lines trialed at 6 to 10 locations each year. Yield was analyzed using mixed models that accounted for experimental design and spatial variations. Genotype-by-sequencing provided nearly 27,000 high-quality SNPs. Average genomic predictive ability, estimated for each year by randomly masking lines as missing …

Women’S Work? The Relationship Between Farmwork And Gender Self-Perception, Jolene Smyth, Alexis Swendener, Emily Kazyak

Department of Sociology: Faculty Publications

Women have long been involved in agricultural production, yet farming and ranching have been associated with masculinity and men. In recent years women have become more involved and more likely to take active and equal roles on farms and ranches and thus increasingly are doing tasks that have been associated with masculinity. Prior work indicates that women are perceived by others as more masculine when they do these tasks, but less work has focused on the association between women’s involvement in farming and women’s own perceptions of their gender (i.e., how masculine or feminine they feel). Using 2006 survey data …

Intermolecular Rna Recombination Occurs At Different Frequencies In Alternate Forms Of Brome Mosaic Virus Rna Replication Compartments, Hernan Garcia-Ruiz, Arturo Diaz, Paul Ahlquist

Department of Plant Pathology: Faculty Publications

Positive-strand RNA viruses replicate their genomes in membrane-bound replication compartments. Brome mosaic virus (BMV) replicates in vesicular invaginations of the endoplasmic reticulum membrane. BMV has served as a productive model system to study processes like virus-host interactions, RNA replication and recombination. Here we present multiple lines of evidence showing that the structure of the viral RNA replication compartments plays a fundamental role and that recruitment of parental RNAs to a common replication compartment is a limiting step in intermolecular RNA recombination. We show that a previously defined requirement for an RNA recruitment element on both parental RNAs is not to …

Metagenomic Analysis Of Viruses Associated With Maize Lethal Necrosis In Kenya, Mwathi Jane Wamaitha, Deepti Nigam, Solomon Maina, Francesca Stomeo, Anne Wangai, Joyce Njoki Njuguna, Timothy A. Holton, Bramwel W. Wanjala, Mark Wamalwa, Tanui Lucas, Appolinaire Djikeng, Hernan Garcia Ruiz

Department of Plant Pathology: Faculty Publications

Background: Maize lethal necrosis is caused by a synergistic co-infection of Maize chlorotic mottle virus (MCMV) and a specific member of the Potyviridae, such as Sugarcane mosaic virus (SCMV), Wheat streak mosaic virus (WSMV) or Johnson grass mosaic virus (JGMV). Typical maize lethal necrosis symptoms include severe yellowing and leaf drying from the edges. In Kenya, we detected plants showing typical and atypical symptoms. Both groups of plants often tested negative for SCMV by ELISA. Methods: We used next-generation sequencing to identify viruses associated to maize lethal necrosis in Kenya through a metagenomics analysis. Symptomatic and asymptomatic leaf samples …

Susceptibility Genes To Plant Viruses, Hernan Garcia Ruiz

Department of Plant Pathology: Faculty Publications

Plant viruses use cellular factors and resources to replicate and move. Plants respond to viral infection by several mechanisms, including innate immunity, autophagy, and gene silencing, that viruses must evade or suppress. Thus, the establishment of infection is genetically determined by the availability of host factors necessary for virus replication and movement and by the balance between plant defense and viral suppression of defense responses. Host factors may have antiviral or proviral activities. Proviral factors condition susceptibility to viruses by participating in processes essential to the virus. Here, we review current advances in the identification and characterization of host factors …

Profile Of Genetically Modified Plants Authorized In Mexico, Mayra Teresa Garcia Ruiz, Aaron N. Knapp, Hernan Garcia Ruiz

Department of Plant Pathology: Faculty Publications

Mexico is a center of origin for several economically important plants including maize, cotton, and cocoa. Maize represents more than a food crop, has been declared a biological, cultural, agricultural and economic patrimony, and is linked to the national identity of Mexicans. In this review, we describe the historic and current use of genetically modified plants in Mexico and factors that contributed to the development of the biosafety regulation. We developed a database containing all permit applications received by the government to release genetically modified plants. A temporal and geographical analysis identified the plant species that have been authorized for …

Genomic-Enabled Prediction Models Using Multi-Environment Trials To Estimate The Effect Of Genotype × Environment Interaction On Prediction Accuracy In Chickpea, Manish Roorkiwal, Diego Jarquin, Muneendra K. Singh, Pooran M. Gaur, Chellapilla Bharadwaj, Abhishek Rathore, Reka Howard, Samineni Srinivasan, Ankit Jain, Vanika Garg, Sandip Kale, Annapurna Chitikineni, Shailesh Tripathi, Elizabeth Jones, Kelly R. Robbins, José Crossa, Rajeev K. Varshney

Department of Agronomy and Horticulture: Faculty Publications

Genomic selection (GS) by selecting lines prior to field phenotyping using genotyping data has the potential to enhance the rate of genetic gains. Genotype × environment (G × E) interaction inclusion in GS models can improve prediction accuracy hence aid in selection of lines across target environments. Phenotypic data on 320 chickpea breeding lines for eight traits for three seasons at two locations were recorded. These lines were genotyped using DArTseq (1.6 K SNPs) and Genotyping-by-Sequencing (GBS; 89 K SNPs). Thirteen models were fitted including main effects of environment and lines, markers, and/or naïve and informed interactions to estimate prediction …

Gene Expression Profiling Of Iron Deficiency Chlorosis Sensitive And Tolerant Soybean Indicates Key Roles For Phenylpropanoids Under Alkalinity Stress, Brian M. Waters, Keenan Amundsen, George L. Graef

Department of Agronomy and Horticulture: Faculty Publications

Alkaline soils comprise 30% of the earth and have low plant-available iron (Fe) concentration, and can cause iron deficiency chlorosis (IDC). IDC causes soybean yield losses of $260 million annually. However, it is not known whether molecular responses to IDC are equivalent to responses to low iron supply. IDC tolerant and sensitive soybean lines provide a contrast to identify specific factors associated with IDC.We used RNA-seq to compare gene expression under combinations of normal pH (5.7) or alkaline pH (7.7, imposed by 2.5mM bicarbonate, or pH 8.2 imposed by 5mM bicarbonate) and normal (25μM) or low (1μM) iron conditions from …

European Spaces And The Roma: Denaturalizing The Naturalized In Online Reader Comments, Theresa Catalano, Grace E. Fielder

Department of Teaching, Learning, and Teacher Education: Faculty Publications

With the entry of several Eastern European nations into the European Union (EU), a “third” space has developed in the discourse for nations perceived as not fully integrated “inside” the EU system. This article investigates the construction of this “third space” in the resultant “moral panic” about undesired immigration from other EU countries and its potential drain on the social services of the United Kingdom and links it to Euroskeptic discourse in British media. The article uses construal operations from cognitive linguistics combined with critical discourse studies as a way of denaturalizing the discourse in online comments that focus on …

Reduction In Musca Domestica Fecundity By Dsrna-Mediated Gene Knockdown, Neil D. Sanscrainte, Hanayo Arimoto, Christy M. Waits, Lucy Y. Li, Dana Johnson, Chris Geden, James Becnel, Alden S. Estep

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

House flies (Musca domestica) are worldwide agricultural pests with estimated control costs at $375 million annually in the U.S. Non-target effects and widespread resistance challenge the efficacy of traditional chemical control. Double stranded RNA (dsRNA) has been suggested as a biopesticide for M. domestica but a phenotypic response due to the induction of the RNAi pathway has not been demonstrated in adults. In this study female house flies were injected with dsRNA targeting actin-5C or ribosomal protein (RP) transcripts RPL26 and RPS6. Ovaries showed highly reduced provisioning and clutch reductions of 94±99% in RP dsRNA treated flies but …

Detection Of Exogenous Double-Stranded Rna Movement In In Vitro Peanut Plants, P. C. Faustinelli, I. L. Power, R. S. Arias

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

New technologies are needed to eliminate mycotoxins and/or fungal pathogens from agricultural products. RNA interference (RNAi) has shown potential to control fungi associated with crops. In RNAi, double-stranded RNA (dsRNA) targets homologous mRNA for cleavage, and can reach the mRNA of pathogens in contact with the plant. The key element in this process is the movement of RNA signals cell-to-cell and over long distances within the plant, and between host plants and parasites.

• In this study, we selected a regulatory gene in the aflatoxin biosynthesis pathway, aflS/ aflR, necessary for the production of aflatoxins in Aspergillus spp. We …

Effects Of Triclosan In Breast Milk On The Infant Fecal Microbiome, Candace S. Bever, Amy A. Rand, Malin Nording, Diana Taft, Karen M. Kalanetra, David A. Mills, Melissa A. Breck, Jennifer T. Smilowitz, J. Bruce German, Bruce D. Hammock

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Triclosan is frequently used for its antimicrobial properties and has been detected in human serum, urine, and breast milk. Animal and molecular studies have shown that triclosan exerts a wide range of adverse health effects at both high (ppm) and low (ppb) concentrations. Since triclosan is of growing concern to human and environmental health, there is a need to improve extraction procedures and to study additional effects from triclosan exposure. In this study, we have improved triclosan extraction from breast milk by using salt (MgSO4) to reduce emulsion formation and increase water polarity and water (~80%) to enhance the overall …

Application Of The Biosafety Ram And Eprotocol Software Programs To Streamline Institutional Biosafety Committee Processes At The Usda-National Animal Disease Center, John P. Bannantine, Karen B. Register, David M. White

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The National Animal Disease Center (NADC) conducts basic and applied research on endemic animal diseases of high priority that adversely affect US livestock production or trade. Experiments conducted at this center vary in range and scope with a subset involving synthetic or recombinant nucleic acids (DNA), microorganisms, and/or animals. Historically, the NADC used hard copy paper and filing systems to catalog and monitor these types of experiments, but to improve communication, tracking, searching, reporting, and documentation of Institutional Biosafety Committee (IBC) actions, this institution has transitioned to using a commercially available software tool to digitally manage protocols in our ongoing …

Knowledge Gaps That Hamper Prevention And Control Of Mycobacterium Avium Subspecies Paratuberculosis Infection, Herman W. Barkema, Karin Orsel, S.S. Nielsen, A.P. Koets, V.P.M.G. Rutten, J. P. Bannantine, G.P. Keefe, D.F. Kelton, Scott J. Wells, Richard J. Whittington, Colin G. Mackintosh, E.J. Manning, M.F. Weber, C. Heuer, T.L. Forde, C. Ritter, S. Roche, C.S. Corbett, R. Wolf, P.J. Griebel, J.P. Kastelic, J. De Buck

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In the last decades, many regional and country-wide control programmes for Johne’s disease (JD) were developed due to associated economic losses, or because of a possible association with Crohn’s disease. These control programmes were often not successful, partly because management protocols were not followed, including the introduction of infected replacement cattle, because tests to identify infected animals were unreliable, and uptake by farmers was not high enough because of a perceived low return on investment. In the absence of a cure or effective commercial vaccines, control of JD is currently primarily based on herd management strategies to avoid infection of …

A Simple And Scalable Hydrogel-Based System For Culturing Protein-Producing Cells, Qiang Li, Qiaofeng Wang, Ou Wang, Kaifeng Shao, Haishuang Lin, Yuguo Lei

Department of Chemical and Biomolecular Engineering: Faculty Publications

Recombinant protein therapeutics have become important components of the modern medicine. Majority of them are produced with mammalian cells that are cultured either through adherent culturing, in which cells are cultured on substrates, or suspension culturing, in which cells are suspended and cultured in agitated cell culture medium in a culture vessel. The adherent cell culturing method is limited by its low yield. In suspension culturing, cells need extensive genetic manipulation to grow as single cells at high density, which is time and labor-consuming. Here, we report a new method, which utilizes a thermoreversible hydrogel as the scaffold for culturing …

Improving The Physical Activity And Outdoor Play Environment Of Family Child Care Homes In Nebraska Through Go Nutrition And Physical Activity Self-Assessment For Child Care, Danae Dinkel, Dipti A. Dev, Yage Guo, Emily Hulse, Zainab Rida, Ami Sedani, Brian Coyle

Department of Child, Youth, and Family Studies: Faculty Publications

Background: The purpose of this study was to determine if the Go Nutrition and Physical Activity Self-Assessment in Child Care (Go NAP SACC) intervention was effective in improving best practices in the areas of infant and child physical activity and outdoor play and learning in family child care homes (FCCHs) in Nebraska. Methods: FCCHs (n = 201) participated in a pre– post evaluation using the Infant and Child Physical Activity and Outdoor Play and Learning assessments from the Go NAP SACC validated measure to assess compliance with best practices. Results: At post, FCCHs demonstrated significant differences in 85% of the …

Resilience In American Indian And Alaska Native Public Health: An Underexplored Framework, Nicolette I. Teufel-Shone, Julie A. Tippens, Hilary C. Mccrary, John E. Ehiri, Priscilla R. Sanderson

Department of Child, Youth, and Family Studies: Faculty Publications

Objective: To conduct a systematic literature review to assess the conceptualization, application, and measurement of resilience in American Indian and Alaska Native (AIAN) health promotion.

Data Sources: We searched 9 literature databases to document how resilience is discussed, fostered, and evaluated in studies of AIAN health promotion in the United States.

Study Inclusion and Exclusion Criteria: The article had to (1) be in English; (2) peer reviewed, published from January 1, 1980, to July 31, 2015; (3) identify the target population as predominantly AIANs in the United States; (4) describe a nonclinical intervention or original research that identified resilience as …

Perfluorinated Alkyl Acids And Fecundity Assessment In Striped Mullet (Mugil Cephalus) At Merritt Island National Wildlife Refuge, Jacqueline T. Bangma, Jessica L. Reiner, Russell H. Lowers, Theresa M. Cantu, Jacob Scott, Jeffrey E. Korte, Doug M. Scheidt, Chris Mcdonough, Jonathan Tucker, Brenton Back, Douglas H. Adams, John A. Bowden

United States National Aeronautics and Space Administration: Publications

This study investigated wild caught striped mullet (Mugil cephalus) at Merritt Island National Wildlife Refuge (MINWR) for levels of 15 perfluoroalkyl acids (PFAA) in tandem with individual fecundity measurements (Oocyte sub-stage 2 late, n=42) and oocyte reproductive stages (Stages 1–5, n=128). PFAAmeasurementswere quantified in stripedmullet liver (n=128),muscle (n=49), and gonad (n=10). No significant negative impacts of liver PFAA burden on wild-caught,mullet fecundity endpoints were observed in this study; however, changes in PFAAwere observed in the liver asmullet progressed through different sub-stages of oocyte development. Of the PFAA with significant changes …

Gene Expression Profiles Of The Small Intestinal Mucosa Of Dogs Repeatedly Infected With The Cestode Echinococcus Multilocularis, Hirokazu Kouguchi, Takao Irie, Jun Matsumoto, Hidefumi Furuoka, Kenji Ishiwata, Ryo Nakao, Kinpei Yagi

Harold W. Manter Laboratory of Parasitology: Library Materials

The data set presented in this article is related to a previous research article entitled, The timing of worm exclusion in dogs repeatedly infected with the cestode Echinococcus multilocularis (Kouguchi et al., 2016). This article describes the genes >2 fold up- or down-regulated in the first- and repeated-infection groups compared to the healthy controls group. The gene expression profiles were generated using the Agilent-021193 Canine (V2) Gene Expression Microarray (GPL15379). The raw and normalized microarray data have been deposited with the Gene Expression Omnibus (GEO) database under accession number GSE105098.

The Interface Between Wheat And The Wheat Curl Mite, Aceria Tosichella, The Primary Vector Of Globally Important Viral Diseases, Anna Skoracka, Brian G. Rector, Gary L. Hein

Department of Entomology: Faculty Publications

Wheat production and sustainability are steadily threatened by pests and pathogens in both wealthy and developing countries. This review is focused on the wheat curl mite (WCM), Aceria tosichella, and its relationship with wheat. WCM is a major pest of wheat and other cereals and a vector of at least four damaging plant viruses (Wheat streak mosaic virus, High plains wheat mosaic virus, Brome streak mosaic virus, and Triticum mosaic virus). The WCM–virus pathosystem causes considerable yield losses worldwide and its severity increases significantly when mixed-virus infections occur. Chemical control strategies are largely ineffective because WCM occupies …

The Influence Of Spatiotemporally Decoupled Land Use On Honey Bee Colony Health And Pollination Service Delivery, Matthew D. Smart, Clint R. V. Otto, Benjamin L. Carlson, Cali L. Roth

Department of Entomology: Faculty Publications

Societal dependence on insects for pollination of agricultural crops has risen amidst concerns over pollinator declines. Habitat loss and lack of forage have been implicated in the decline of both managed and native pollinators. Land use changes in the Northern Great Plains of the US, a region supporting over 1 million honey bee colonies annually, have shifted away from historical grassland ecosystems bees rely on for forage toward landscapes dominated by corn, soybeans, and other row crops. We investigated how land use impacts honey bee colony population size during the growing season and subsequent colony population size for almond pollination …

Parental Rnai Suppression Of Chromatin Remodeling Genes To Control Coleopteran Pests, Blair D. Siegfried, Kenneth E. Narva, Kanika Arora, Sarah E. Worden, Chitvan Khajuria, Elane Fishilevich, Nicholas P. Storer, Meghan Frey, Ronda L. Hamm, Ana Maria Velez

Department of Entomology: Faculty Publications

This disclosure concerns nucleic acid molecules and meth ods of use thereof for control of hemipteran pests through RNA interference - mediated inhibition of target coding and transcribed non - coding sequences in hemipteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of hemipteran pests, and the plant cells and plants obtained thereby.

Mirdis: A Web Tool For Endogenous And Exogenous Microrna Discovery Based On Deep-Sequencing Data Analysis, Hanyuan Zhang, Bruno Vieira Resende E Silva, Juan Cui

School of Computing: Faculty Publications

Small RNA sequencing is the most widely used tool for microRNA (miRNA) discovery, and shows great potential for the efficient study of miRNA cross-species transport, i.e., by detecting the presence of exogenous miRNA sequences in the host species. Because of the increased appreciation of dietary miRNAs and their far-reaching implication in human health, research interests are currently growing with regard to exogenous miRNAs bioavailability, mechanisms of cross-species transport and miRNA function in cellular biological processes. In this article, we present microRNA Discovery (miRDis), a new small RNA sequencing data analysis pipeline for both endogenous and exogenous miRNA detection. Specifically, we …