Digital Commons Network^™

Phenotypes Of Macrophages Present In The Intestine Are Impacted By Stage Of Disease In Cattle Naturally Infected With Mycobacterium Avium Subsp. Paratuberculosis, Caitlin J. Jenvey, Adrienne L. Shircliff, J. P. Bannantine, Judith R. Stabel

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Macrophages play an important role in the host immune response to Mycobacterium avium subsp. paratuberculosis (MAP) infection, however, MAP is able to disrupt normal macro- phage functions to avoid destruction. It is unclear whether the phenotypes of macrophages present in the target tissue play a role in the inability to clear MAP infection. The aim of this study was to identify macrophage phenotypes (host defense or resolution and repair) present within the bovine ileum of naturally infected cattle, as well as to ascertain abundance of each macrophage phenotype present during different stages of MAP infection. Immunofluo- rescent (IF) labeling was …

Predicting Spatial Risk Of Wolf-Cattle Encounters On Rugged And Extensive Grazing Lands, Patrick R. Clark, Joe Chigbrow, Douglas E. Johnson, John Williams, Larry L. Larson, Tyanne Roland

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Cattle grazing lands in the mountainous western United States are rugged, complex, and extensive. Terrain, vegetation, and other landscape features vary greatly across space. Risk of wolf-cattle encounters and potential for depredation loss certainly differ spatially as consequence of this variability. Yet, our understanding of this spatial risk is quite poor and this knowledge gap severely hampers our abilities to manage wolf-livestock interactions and mitigate conflicts. During 2009-2011, a research study was conducted at four study areas (USFS cattle grazing allotments) in western Idaho to evaluate and predict risk of wolf-cattle encounters. Each year, a random sample of 10 lactating …

Characterization Of Ethanol Extracted Cell Wall Components Of Mycobacterium Avium Subsp. Paratuberculosis, John P. Bannantinea, Ashutosh Wadhwa, Judith R. Stabel, Shigetoshi Eda

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Antigens extracted using ethanol (EtOH) and incorporated in the EtOH vortex ELISA (EVELISA) test have previously shown high specificity and sensitivity for detecting Mycobacterium avium subspecies paratuberculosis (Map) and M. bovis infections in cattle. The objective of this study is to define the components present in the EtOH extract. We show that this extract is composed of lipid, carbohydrate, and proteins on the surface of the bacilli, and that EtOH removes the outer layer structure of Map which comprise these elements. To identify proteins, polyclonal antibodies to the EtOH prep were produced and used to screen a Map genomic expression …

Wearable Devices For Single Cell Sensing And Transfection, Lingqian Chang, Yu-Chieh Wang, Faheem Ershad, Ruiguo Yang, Cunjiang Yu, Yubo Fan

Department of Mechanical and Materials Engineering: Faculty Publications

Transdermal drug delivery systems have been successful for decades. Now these devices can be further scaled down, and their applications have been extended to wide selections of cargo, ranging from natural molecules (e.g., insulin and glucose) to bioengineered molecules (e.g., nanoparticles and vaccines). Some emerging nanopatches show promise for precise single-cell gene transfection in vivo and have advantages over conventional tools in terms of delivery efficiency, safety, and controllability of delivered dose. In this review, we discuss recent technical advances in wearable micro/nano devices with unique capabilities or potential for single-cell biosensing and transfection in the skin or other organs, …

The Genera Of Agaocephalini (Coleoptera: Scarabaeidae: Dynastinae) Of Colombia, With Description Of A New Species Of Lycomedes Breme, Jhon César Neita-Moreno, Brett C. Ratcliffe

Department of Entomology: Faculty Publications

The six genera of Agaocephalini in Colombia are distinguished in a dichotomous key and illustrated. Lycomedes enigmaticus Neita-Moreno and Ratcliffe, new species, is described and differentiated by character comparisons with other previously known Colombian species. Lycomedes lydiae Arnaud, 2012 is proposed as a new junior synonym of Lycomedes reichei Brême, 1844.

Los seis géneros de Agaocephalini en Colombia son incluidos e ilustrados en una clave dicotómica. Se describe Lycomedes enigmaticus Neita-Moreno y Ratcliffe, especie nueva y es diferenciada por comparación de caracteres con otras especies previamente conocidas de Colombia. Se propone Lycomedes lydiae Arnaud, 2012 como un nuevo sinónimo …

Insights Into Gemcitabine Resistance And The Potential For Therapeutic Monitoring, Teklab Gebregiworgis, Fatema Bhinderwala, Vinee Purohit, Nina V. Chaika, Pankaj K. Singh, Robert Powers

Department of Chemistry: Faculty Publications

Introduction—Gemcitabine is an important component of pancreatic cancer clinical management. Unfortunately, acquired gemcitabine resistance is widespread and there are limitations to predicting and monitoring therapeutic outcomes.

Objective—To investigate the potential of metabolomics to differentiate pancreatic cancer cells that develops resistance or respond to gemcitabine treatment.

Results—We applied 1D ¹H and 2D ¹H-¹³C HSQC NMR methods to profile the metabolic signature of pancreatic cancer cells. ¹³C₆-glucose labeling identified thirty key metabolites uniquely altered between wild-type and gemcitabine-resistant cells upon gemcitabine treatment. Gemcitabine resistance was observed to reprogram glucose metabolism and to enhance the pyrimidine …

Masthead & Table Of Contents, Vol. 97, No. 4

Nebraska Law Review

No abstract provided.

Amex And Post-Cartesian Antitrust, Justin (Gus) Hurwitz

Nebraska Law Review

I. Introduction

II. Situating American Express ... A. Different Sides of the American Express Opinion ... 1. Two-Sided Markets ... 2. The American Express Opinion ... B. The Real Issue Is Messy, Not Two-Sided, Markets … C. The Many Messes of Modern Markets

III. Competition in Messy Markets ... A. Simple Competition in Simple Markets ... B. More Complex Competition in Messier Markets ... C. American Express: The Competition Is in the Pudding

IV. The Many Sides of AmEx’s Rightness ... A. A Burden Best Born by Plaintiffs ... B. Economic Theory as a Question of Law or …

Nebraska's Advantage: Productive Agriculture And Bountiful Natural Resources, Laura E. Young, Mary Garbacz, Michael J. Boehm, Bethany Karlberg, John Carroll, Kathryn Bagniewski, Daran Rudnick, Halle Ramsey, Marc Libault, Cadrien Livingston, Jamilynn Poletto, Leanne Gamet, Troy Gilmore, Natalie Jones, Lisa Pennisi, Elizabeth Vanwormer, Abby Steffen, Andrea Basche, Rylie Kalb, Rick Koelsch, Bethany Karlberg, Martha Shulski, Michael Hayes, Peter Mccornick, Amit Jhala, Walter Schacht, Stanosh Pitla, Michael Ferguson, John Gamon, Joe Luck, Tami M. Brown-Brandl, Bertrand Clarke, Taro Mieno

Strategic Discussions for Nebraska

Nebraska's Advantage: Productive Agriculture and Bountiful Natural Resources

You can’t talk about healthy production systems and natural resource systems without also thinking about the resilience. We need to harmonize production agriculture in a way that ensures the resilience of our natural ecosystems and rural communities—all while addressing bundles of grand challenges.

Nebline, January 2019

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

2018 Extension highlights

Benefit to people

Teaching, growing and making a difference

36,520 youth engaged in 4-H programs

Extension’s volunteers worth nearly $700,000

New Year, new you: Nutrition tips to a healthier you

RECIPE OF THE MONTH

Pesticide applicator 2019 training dates

Ag Estate and Succession Planning workshop, Jan. 23 & March 5

Successful Farmer Series

Upcoming green industry conferences

Extension’s 2019 Master Gardener trainings, two sites available

Garden Guide Things to Do This Month

Sticky situation: Using glue boards to monitor pests

HEART OF 4-H Volunteer Award: Susan Bulling

IT’S TIME TO RE-ENROLL IN 4-H!

Meet 2018–2019 4-H Council …

Ncuwm Talk Abstracts 2019

Nebraska Conference for Undergraduate Women in Mathematics

No abstract provided.

Past Invasive Species Advisory Committee (Isac) Members, National Invasive Species Committee

National Invasive Species Council

United States Department of the Interior

National Invasive Species Council

Invasive Species Advisory Committee (ISAC)

Past ISAC Members:

Class 9 (2016-2019)

Class 8 (2011-2016/2017)

Class 7 (2011-2012/2014)

Class 6 (2009-2011/2012)

Class 5 (2008/2009-2010)

Class 4 (2006-2008)

Class 3 (2004-2006)

Class 2 (2002-2004)

Class 1 (1999-2001)

Age-0 Walleye Sander Vitreus Display Length-Dependent Diet Shift To Piscivory, Christopher S. Uphoff, Casey W. Schoenebeck, Keith D. Koupal, Kevin L. Pope, W. Wyatt Hoback

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

The ontogenetic diet shift to piscivory can be energetically beneficial for fish growth and allows larger, more energetically profitable prey to be consumed. A shift to piscivory may be easier for longer individuals within a cohort due to larger gape size, and an early shift is likely advantageous, potentially leading to increased growth rates and survival. Such length-dependent ontogenetic diet shifts may explain the intracohort variability in length that is common for age-0 walleye (Sander vitreus). The objectives of this study were to describe seasonal intracohort variability in length, identify the timing of the shift to piscivory and …

Ecosystem Size Predicts Social-Ecological Dynamics, Mark A. Kaemingk, Christopher J. Chizinski, Craig R. Allen, Kevin L. Pope

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Recreational fisheries are complex adaptive systems that are inherently difficult to manage because of heterogeneous user groups (consumptive vs. nonconsumptive) that use patchily distributed resources on the landscape (lakes, rivers, coastlines). There is a need to identify which system components can effectively predict and be used to manage nonlinear and cross-scale dynamics within these systems. We examine how ecosystem size or water body size can be used to explain complicated and elusive angler-resource dynamics in recreational fisheries. Water body size determined angler behavior among 48 Nebraska, U.S.A. water bodies during an 11- year study. Angler behavior was often unique and …

Representation Of Unaccompanied Migrant Children From Central America In The United States: Media Vs. Migrant Perspectives, Theresa Catalano, Jessica Mitchell-Mccollough

Department of Teaching, Learning, and Teacher Education: Faculty Publications

This chapter examines the representation of unaccompanied minors fleeing Central America (namely Honduras, Guatemala and El Salvador) in U.S. online national news sources over a one-year period and compares this to the way these children talk about their own perceptions of migration and their motivation for moving. Data collection consisted of online news reports on unaccompanied minors from Central America in the United States as well as interviews with children collected from the United Nations Refugee Agency (UNHCR) and other humanitarian organizations. Multimodal critical discourse analysis reveals a qualitative difference in discourse (e.g., use of metaphor, metonymy, deixis and visual …

2019 Annual Report, Ben Bohall, John Erixson

Nebraska Forest Service: Publications

No abstract provided.

2019 Annual Report Nebraska Game And Parks

Nebraska Game and Parks Commission: Publications

No abstract provided.

How Do Teachers Use Comics To Promote Engagement, Equity, And Diversity In Science Classrooms?, Camilla Matuk, Talia Hurwich, Amy Spiegel, Judy Diamond

World of Viruses

Equitable learning opportunities are critical to the goals of science education. However, major curriculum standards are vague on how to achieve equity goals, and educators must often develop their own resources and strategies to achieve equity goals. This study examines how educators used a comic book series designed to interest youth in virology as a way to make science more broadly appealing to their diverse students. We begin with the notion of Pedagogical Design Capacity, which describes a dynamic relationship between teachers and their tools and the ability for teachers to perceive and leverage affordances of artifacts as tools in …

Drought Impacts And Management In Prairie And Sandhills State Parks, Theresa M. Jedd, Devarati Bhattacharya, Cara Pesek, Michael J. Hayes

National Oceanographic and Atmospheric Administration: Technical Reports and Related Materials

The combination of decreased water availability and increased temperatures can interfere with outdoor activities, particularly with surface water decline and the increased risk of wildfire. Drought is a longer-term climate trend, but there is a tendency toward short-term reactions only when a drought occurs. Policy mechanisms for drought, where present, are often left to managerial discretion because they are not needed every year, and lack specific indicators. Recreator choices contribute to adaptation and public lands managers also shape drought response by monitoring meteorological trends and managing resources wisely. Despite these trends, no singular recreational drought definition exists. To understand socio-environmental …

Drought Impacts And Management In Prairie And Sandhills State Parks, Theresa M. Jedd, Devarati Bhattacharya, Cara Pesek, Michael J. Hayes

National Oceanographic and Atmospheric Administration: Technical Reports and Related Materials

The combination of decreased water availability and increased temperatures can interfere with outdoor activities, particularly with surface water decline and the increased risk of wildfire. Drought is a longer-term climate trend, but there is a tendency toward short-term reactions only when a drought occurs. Policy mechanisms for drought, where present, are often left to managerial discretion because they are not needed every year, and lack specific indicators. Recreator choices contribute to adaptation and public lands managers also shape drought response by monitoring meteorological trends and managing resources wisely. Despite these trends, no singular recreational drought definition exists. To understand socio-environmental …

Melanism As A Potential Thermal Benefit In Eastern Fox Squirrels (Sciurus Niger), Amanda K. Ciurej, Ashley Oblander, Andrew W. Swift, James A. Wilson

United States Fish and Wildlife Service: Publications

Melanistic fox squirrels (Sciurus niger) have expanded westward and increased in frequency in the Omaha, Nebraska, and Council Bluffs, Iowa, metropolitan areas. The selective advantage of melanism is currently unknown, but thermal advantages have been hypothesized, especially in winter. No difference in metabolic response curves were measured between melanistic (black) and rufus (orange) fox squirrels. When exposed to sunny skies, both melanistic and rufus squirrels had higher surface (skin and fur) temperature as ambient temperatures increased. Melanistic squirrel surface temperatures did not differ when squirrels were exposed to sunny or cloudy skies. However, rufus individuals showed significantly lower …

Multifocal Necrotizing Myopathy In Northern Elephant Seal (Mirounga Angustirostris) Pups, San Miguel Island, California, Terry R. Spraker, Tetiana A. Kuzmina, Eugene T. Lyons, Robert L. Delong, Claire Simeone, D. N. Rao Veeramachaneni

United States Department of Commerce: Staff Publications

A field study addressing causes of mortality in freshly dead northern elephant seals (Mirounga angustirostris, Gill, 1866) was conducted on San Miguel Island, California, in February 2015. Necropsies were performed on 18 pups ranging in age from stillbirths to approximately 7 to 8 weeks. The primary gross diagnoses in these pups included trauma, myopathy, starvation/ emaciation, infections, congenital anomalies, and perinatal mortality. However, 6 (33%) had a previously unrecognized myopathy characterized by multiple white streaks that were most obvious within the inner layer of the abdominal wall and the small innermost ventral intercostal muscles. Following histological examination, 2 …

Novel Amphiphilic Cyclobutene And Cyclobutane Cis-C18 Fatty Acid Derivatives Inhibit Mycobacterium Avium Subsp. Paratuberculosis Growth, Denise K. Zinniel, Wantanee Sittiwong, Darrell D. Marshall, Govardhan Rathnaiah, Isin T. Sakallioglu, Robert Powers, Patrick H. Dussault, Raul G. Barletta

School of Veterinary and Biomedical Sciences: Faculty Publications

Mycobacterium avium subspecies paratuberculosis (Map) is the etiologic agent of Johne’s disease in ruminants and has been associated with Crohn’s disease in humans. An effective control of Map by either vaccines or chemoprophylaxis is a paramount need for veterinary and possibly human medicine. Given the importance of fatty acids in the biosynthesis of mycolic acids and the mycobacterial cell wall, we tested novel amphiphilic C₁₀ and C₁₈ cyclobutene and cyclobutane fatty acid derivatives for Map inhibition. Microdilution minimal inhibitory concentrations (MIC) with 5 or 7 week endpoints were measured in Middlebrook 7H9 base broth media. We compared the …

Investigation Of Congestive Heart Failure In Beef Cattle In A Feedyard At A Moderate Altitude In Western Nebraska, Rodney A. Moxley, David R. Smith, Dale M. Grotelueschen, Tom Edwards, David Steffen

School of Veterinary and Biomedical Sciences: Faculty Publications

Right-sided congestive heart failure (brisket disease) commonly occurs in cattle raised at elevations >2,500– 3,500 m. We investigated clinical cases resembling brisket disease at a western Nebraska feedyard at a moderate altitude (1,369 m). Over a 15-mo period (2009–2010), we examined 17 cases (16 steers and 1 heifer), all purebred Angus. All animals had clinical right-sided heart failure: brisket and ventral abdominal edema, and severe chronic passive congestion of the liver. Gross examination confirmed right ventricular hypertrophy (left ventricle plus septum: right ventricle weight ratio mean: 1.33 vs. 2.8–4.0 reference interval). Microscopically, all 17 cases had interstitial fibrosis (mean score: …

Droughtscape, Winter 2019, National Drought Mitigation Center

DroughtScape: Quarterly Newsletter of the National Drought Mitigation Center (2007–2023)

Contents

From the Director

Drought intensified in California and Nevada, eased elsewhere

Year in Review: Drought spread and intensified in the West; record precipitation in the East

Drought impact summary for 4th quarter 2018

Drought impact summary 2018

Five states began drought plan updates in 2018

New web-based form makes submitting drought observations easier

FEMA risk assessment process tailored for drought

Upcoming events

Thymic Expression Of Il-4 And Il-15 After Systemic Inflammatory Or Infectious Th1 Disease Processes Induce The Acquisition Of"Innate" Characteristics During Cd8 + T Cell Development, Natalia S. Baez, Fabio Cerbán, Constanza Savid-Frontera, Deborah L. Hodge, Jimena Tosello, Eva Acosta-Rodriguez, Laura Almada, Adriana Gruppi, Maria Estefania Viano, Howard A. Young, Maria Cecilia Rodriguez-Galan

United States Public Health Resources

Innate CD8 + T cells express a memory-like phenotype and demonstrate a strong cytotoxic capacity that is critical during the early phase of the host response to certain bacterial and viral infections. These cells arise in the thymus and depend on IL-4 and IL-15 for their development. Even though innate CD8 + T cells exist in the thymus of WT mice in low numbers, they are highly enriched in KO mice that lack certain kinases, leading to an increase in IL-4 production by thymic NKT cells. Our work describes that in C57BL/6 WT mice undergoing a Th1 biased infectious disease, …

Insect Profiles, Eric Honerlaw

Department of Entomology: Distance Master of Science Projects

Profiled species: Aerial Yellow Jacket • American Carrion Beetle • Black Imported Fire Ant • Blue Mud Dauber • Camel Cricket • Case Making Clothes Moth - Done • Colorado Potato Beetle • Dark Eyed Fruit Fly • Downy Yellow Jacket • Eastern Treehole Mosquito • European Hornet • Evergreen Bagworm • Four-Lined Silverfish • Fruit Fly • German Yellow Jacket • Gray Silverfish • Great Golden Digger Wasp2 • Ground Yellow Jacket • Hister Beetle • Kissing Bug • Larder Beetle • Larger Yellow Ant • Lesser House Fly • Margined Blister Beetle • Masked Hunter • Mason Bee …

Teaching Insetcs: A Four Lesson Plan To Introduce Insects To Kids, Heather Wilkins

Department of Entomology: Distance Master of Science Projects

This teaching guide is meant to be flexible for grades, time, and scope. The lesson plan is designed for grades 2 – 3, but with additional materials can be expounded on for higher grades or simplified for lower grades. It can be used by utilizing all the suggested materials, assignments, and supplements, or can be easily pared down if say you can’t find a book or don’t have internet access for videos. It is a compilation of videos, books, printable pages, interactive activities, and experiments to teach children using fun and interesting methods. It approaches insects with methods for kinetic, …

Integrated Pest Management Manual For Greenhouses, Evy Z. Santiago

Department of Entomology: Distance Master of Science Projects

The original definition of Integrated Pest Management (IPM) was introduced by Stern et al. in 1959 as Integrated Control. It was defined as applied pest control which combines and integrates biological and chemical control (Stern et al. 1959). Entomologists began exploring insect management alternatives to reduce reliance on insecticides in the 1950’s after resistance and pest resurgence in agriculture was first noted. Today, the European Union follows a definition of IPM largely inspired from the definition given by the Food and Agriculture Organization of the United Nations (FAO) (Barzman et al. 2015). Integrated Pest Management means the careful consideration of …

Building An Integrated Pest Management Plan For A Unique Urban Farming Program, Jeff Coco

Department of Entomology: Distance Master of Science Projects

The summer of 2018 led to an opportunity with a local non-profit called Fleet Farming. This non-profit had different departments available for internships which included greenhouse and farming. I was familiar with the program in that I knew they farmed in people’s front yards, in ‘farmlettes’ that had been converted from lawns. I was most interested in the job description under farming that said “practicing IPM”. I had developed an interest in IPM when I took Dr. Tom Weissling’s class on the Management of horticultural crop insects in the Spring of 2018. I wanted more ‘hands on’ learning and this …