Digital Commons Network^™

Injury Scores And Spatial Responses Of Wolves Following Capture: Cable Restraints Versus Foothold Traps, Eric M. Gese, Patricia A. Terletzky, John D. Erb, Kevin C. Fuller, Jeffery P. Grabarkewitz, John P. Hart, Carolin Humpal, Barry A. Sampson, Julie K. Young

United States Department of Agriculture Wildlife Services: Staff Publications

Wolves (Canis lupus) have been captured with foothold traps for several decades to equip them with radiocollars for population monitoring. However, trapping in most areas is limited to spring, summer, and autumn as cold winter temperatures can lead to frozen appendages in trapped animals. In addition, conflicts arise when domestic dogs encounter these traps in nonwinter seasons. An alternative capture method is the use of cable restraint devices (modified neck snares) in the winter. We evaluated injury scores, movement patterns, and space use of wolves captured in cable restraint devices and foothold traps in north central Minnesota, USA, during 2012–2016. …

Assessing Public Support For Restrictions On Transport Of Invasive Wild Pigs (Sus Scrofa) In The United States, Meredith J. Grady, Erin E. Harper, Keith M. Carlisle, Karina H. Ernst, Stephanie A. Shwiff

United States Department of Agriculture Wildlife Services: Staff Publications

Wild pigs (Sus scrofa) are a non-native invasive species in the United States that cause significant economic loss, transmit disease, and inflict damage upon natural resources, agriculture, livestock, and property. Geographic distribution of wild pigs in the United States has nearly tripled since 1982, with anthropogenic influences playing a significant role in the expansion. In this regard, there is speculation that a driver of the expansion may be human-mediated movement of wild pigs to new areas for the purpose of sport hunting. In response, states have implemented a variety of wild pig control policies, including legal restrictions on their transport. …

Race-Of-Virtual-Interviewer Effects, Frederick Conrad, Michael Schober, Daniel Nielsen, Heidi Reichert

Interviewer Workshop, 2019: Interviewers and Their Effects from a Total Survey Error Perspective

In developing self-administered interviewing systems that go beyond text, survey designers are faced with choices about how to represent the interviewing agent. In speech-dialog systems like ACASI and IVR, designers must decide if the voice that presents the spoken questions is unambiguously male or female, whether the pronunciation is regionally marked, etc. Any visual representation of an interviewer (e.g., a photograph, a video) requires designers to choose features that visually convey demographic features like race, gender, age, etc. Here we investigate whether the representation of animated virtual interviewers (VIs) affects responses in the same way that analogous attributes of human …

Limits To The World’S Green Water Resources For Food, Feed, Fiber, Timber, And Bioenergy, Joep F. Schyns, Arjen Y. Hoekstra, Martijn J. Booij, Rick J. Hogeboom, Mesfin Mekonnen

Daugherty Water for Food Global Institute: Faculty Publications

Green water––rainfall over land that eventually flows back to the atmosphere as evapotranspiration––is the main source of water to produce food, feed, fiber, timber, and bioenergy. To understand how freshwater scarcity constrains production of these goods, we need to consider limits to the green water footprint (WFg), the green water flow allocated to human society. However, research traditionally focuses on scarcity of blue water––groundwater and surface water. Here we expand the debate on water scarcity by considering green water scarcity (WSg). At 5 × 5 arc-minute spatial resolution, we quantify WFg and the maximum sustainable level to this footprint (WFg,m), …

Effects Of Land Use On Greenhouse Gas Flux In Playa Wetlands And Associated Watersheds In The High Plains, Usa, Dale W. Daniel, Loren M. Smith, Scott T. Mcmurry, Brian A. Tangen, Charles F. Dahl, Ned H. Euliss Jr., Ted Lagrange

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

In the High Plains, U.S., native prairie conversion to cropland agriculture has resulted in a loss of service delivery capabilities from most depressional wet-lands as a result of sedimentation. Restoring historic hydrological conditions to affected wetlands may rejuvenate some services, however, there may be tradeoffs due to emissions of CH4 and N2O. We evaluated the influence of two predominant conservation programs (Wetlands Reserve Program, WRP and Conservation Reserve Program, CRP) on gas emissions (CO2, CH4, N2O) from 42 playas and uplands in the High Plains of Nebraska. Because playa restoration through the WRP is most prevalent in the Rainwater Basin …

Sodium-Activated Potassium Channels Shape Peripheral Auditory Function And Activity Of The Primary Auditory Neurons In Mice, Daniël O.J. Reijntjes, Jeong Han Lee, Seojin Park, Nick M.A. Schubert, Marcel Van Tuinen, Sarath Vijayakumar, Timothy A. Jones, Sherri M. Jones, Michael Anne Gratton, Xiao-Ming Xia, Ebenezer N. Yamoah, Sonja J. Pyott

Department of Special Education and Communication Disorders: Faculty Publications

potassium (K+) channels shape the response properties of neurons. Although enormous progress has been made to characterize K+ channels in the primary auditory neurons, the molecular identities of many of these channels and their contributions to hearing in vivo remain unknown. Using a combination of RNA sequencing and single molecule fluorescent in situ hybridization, we localized expression of transcripts encoding the sodium-activated potassium channels KNa1.1 (SLO2.2/Slack) and KNa1.2 (SLO2.1/Slick) to the primary auditory neurons (spiral ganglion neurons, SGNs). To examine the contribution of these channels to function of the sGNs in vivo, we measured auditory brainstem responses in KNa1.1/1.2 double …

A New Genus And Seven New Species Of Onciderini Thomson, 1860 (Coleoptera: Cerambycidae: Lamiinae) From Central And South America, Eugenio H. Nearns, Francisco E. De L. Nascimento

Insecta Mundi

Thomsonista Nearns and Nascimento, a new genus of Onciderini Thomson, 1860 (Coleoptera: Cerambycidae: Lamiinae), is described and illustrated. Seven new species of Onciderini are described and illustrated: Hesychotypa antonkozlovi from Ecuador; Hesychotypa danilevskyi from Panama; Lingafelteria pandolfii from Brazil; Oncideres antonkozlovi and Oncideres erwini from Peru; Oncideres johnmarvini from Costa Rica and Panama; and Thomsonista antonkozlovi from Colombia.

Thomsonista Nearns y Nascimento, un nuevo género de Onciderini Thomson, 1860 (Coleoptera, Cerambycidae, Lamiinae), es descrito e ilustrado. Siete nuevas especies de Onciderini son descritas e ilustradas: Hesychotypa antonkozlovi de Ecuador; Hesychotypa danilevskyi de Panamá; Lingafelteria pandolfii de Brasil; Oncideres antonkozlovi y …

Global Responses Of Resistant And Susceptible Sorghum (Sorghum Bicolor) To Sugarcane Aphid (Melanaphis Sacchari), Hannah M. Tetreault, Sajjan Grover, Erin Scully, Tammy Gries, Nathan A. Palmer, Gautam Sarath, Joe Louis, Scott E. Sattler

Department of Entomology: Faculty Publications

The sugarcane aphid (Melanaphis sacchari) has emerged as a significant pest for sorghum. The use of sugarcane aphid-resistant sorghum germplasm with integrated pest management strategies appears to be an excellent solution to this problem. In this study, a resistant line (RTx2783) and a susceptible line (A/BCK60) were used to characterize the differences in plant responses to the sugarcane aphid through a series of experiments, which examined global sorghum gene expression, aphid feeding behavior and inheritance of aphid resistance. The global transcriptomic responses to sugarcane aphids in resistant and susceptible plants were identified using RNA-seq and compared to the …

Maternal Inflammation At Midgestation Impairs Subsequent Fetal Myoblast Function And Skeletal Muscle Growth In Rats, Resulting In Intrauterine Growth Restriction At Term, Caitlin N. Cadaret, Robert J. Posont, Kristin A. Beede, Hannah E. Riley, John Dustin Loy, Dustin T. Yates

Department of Animal Science: Faculty Publications

Maternal inflammation induces intrauterine growth restriction (MI-IUGR) of the fetus, which compromises metabolic health in human offspring and reduces value in livestock. The objective of this study was to determine the effect of maternal inflammation at midgestation on fetal skeletal muscle growth and myoblast profiles at term. Pregnant Sprague-Dawley rats were injected daily with bacterial endotoxin (MI-IUGR) or saline (controls) from the 9th to the 11th day of gestational age (dGA; term = 21 dGA). At necropsy on dGA 20, average fetal mass and upper hindlimb cross-sectional areas were reduced (P < 0.05) in MI-IUGR fetuses compared with controls. MyoD+ and myf5+ myoblasts were less abundant (P < 0.05), and myogenin+ myoblasts were more abundant (P < 0.05) in MI-IUGR hindlimb skeletal muscle compared with controls, indicating precocious myoblast differentiation. Type I and Type II hindlimb muscle fibers were smaller (P < 0.05) in MI-IUGR fetuses than in controls, but fiber type proportions did not differ between experimental groups. Fetal blood plasma TNFα concentrations were below detectable amounts in both experimental groups, but skeletal muscle gene expression for the cytokine receptors TNFR1, IL6R, and FN14 was greater (P < 0.05) in MI-IUGR fetuses than controls, perhaps indicating enhanced sensitivity to these cytokines. Maternal blood glucose concentrations at term did not differ between experimental groups, but MI-IUGR fetal blood contained less (P < 0.05) glucose, cholesterol, and triglycerides. Fetal-to-maternal blood glucose ratios were also reduced (P < 0.05), which is indicative of placental insufficiency. Indicators of protein catabolism, including blood plasma urea nitrogen and creatine kinase, were greater (P < 0.05) in MI-IUGR fetuses than in controls. From these findings, we conclude that maternal inflammation at midgestation causes muscle-centric fetal programming that impairs myoblast function, increases protein catabolism, and reduces skeletal muscle growth near term. Fetal muscle sensitivity to inflammatory cytokines appeared to be enhanced after maternal inflammation, which may represent a mechanistic target for improving these outcomes in MI-IUGR fetuses.

Aesthetics, Ethics, And Lose-Lose Dilemmas In The Anthropocen, Adam Liska

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Anthropogenic climate change will cause violence to increase globally, and nonlinear increases in sea level could cause a major escalation in global conflict. After 2100, a 22-meter sea-level rise is estimated here to dislocate two billion people from coastal areas. These impending civilization-changing events require us to again reevaluate our prevalent aesthetic preferences for luxury that produce a significant fraction of anthropogenic greenhouse gas emissions. Due to industrial inertia, a central dilemma discussed here is the contradictions between the goals and impacts of aesthetics and ethics. Either we lose our own well-being (a loss of high-emission aesthetics) for the benefit …

Next-Generation Sequencing Identifies Polyunsaturated Fatty Acid Responsive Genes In The Juvenile Rat Cerebellum, Aaron A. Mehus, Aaron M. Dickey, Timothy P. L. Smith, Kathleen M. Yeater, Matthew Picklo

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Dietary n-3 polyunsaturated fatty acids (PUFA) influence postnatal brain growth and development. However, little data exist regarding the impacts of dietary n-3 PUFA in juvenile animals post weaning, which is a time of rapid growth. We tested the hypothesis that depleting dietary n-3 PUFA would result in modifications to the cerebellar transcriptome of juvenile rats. To test this hypothesis, three week old male rats (an age that roughly corresponds to an 11 month old child in brain development) were fed diets containing either soybean oil (SO) providing 1.1% energy from α-linolenic acid (ALA; 18:3n-3; ALA-sufficient) or corn oil (CO) providing …

Divergent Switchgrass Cultivars Modify Cereal Aphid Transcriptomes, Kyle G. Koch, Erin D. Scully, Nathan A. Palmer, Scott M. Geib, Gautam Sarath, Tiffany Heng-Moss, Jeff D. Bradshaw

Department of Entomology: Faculty Publications

Schizaphis graminum Rondani (Hemiptera: Aphididae) and Sipha flava Forbes (Hemiptera: Aphididae) are two common pests of bioenergy grasses. Despite the fact that they are both considered generalists, they differ in their ability to colonize Panicum virgatum cultivars. For example, S. flava colonizes both P. virgatum cv. Summer and P. virgatum cv. Kanlow whereas S. graminum can only colonize Summer. To study the molecular responses of these aphids to these two switchgrass cultivars, we generated de novo transcriptome assemblies and compared the expression profiles of aphids feeding on both cultivars to profiles associated with feeding on a highly susceptible sorghum host …

Wyoming’S Ucross Ranch: Its Birds, History, And Natural Environment, Jacqueline Lee Canterbury, Paul Johnsgard

Zea E-Books Collection

This book profiles 60 of the most abundant, characteristic, and interesting birds that have been regularly reported from the Ucross Ranch and the adjacent Powder River Basin. The 20,000-acre Ucross Ranch lies on the western edge of the Powder River Basin of northeastern Wyoming. Ucross is a textbook example of the prairie grassland/ shrubland habitat type referred to as the sagebrush steppe, a landscape that is an icon of Wyoming’s vast open spaces. We focus especially on those species that occur year-round or are present as breeders during the summer months, and we place emphasis on a unique group of …

Global Disease Outbreaks Associated With The 2015–2016 El Niño Event, Assaf Anyamba, Jean-Paul Chretien, Seth C. Britch, Radina P. Soebiyanto, Jennifer L. Small, Rikke Jepsen, Brett M. Forshey, Jose L. Sanchez, Ryan D. Smith, Ryan Harris, Compton J. Tucker, William B. Karesh, J. Linthicum

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Interannual climate variability patterns associated with the El Niño-Southern Oscillation phenomenon result in climate and environmental anomaly conditions in specific regions worldwide that directly favor outbreaks and/or amplification of variety of diseases of public health concern including chikungunya, hantavirus, Rift Valley fever, cholera, plague, and Zika. We analyzed patterns of some disease outbreaks during the strong 2015–2016 El Niño event in relation to climate anomalies derived from satellite measurements. Disease outbreaks in multiple El Niño-connected regions worldwide (including Southeast Asia, Tanzania, western US, and Brazil) followed shifts in rainfall, temperature, and vegetation in which both drought and flooding occurred in …

The Nature Of Science As A Foundation For Fostering A Better Understanding Of Evolution, Craig E. Nelson, Lawrence C. Scharmann, Jean Beard, Lawrence I. Flammer

Department of Teaching, Learning, and Teacher Education: Faculty Publications

Misunderstandings of the nature of science (NOS) contribute greatly to resistance to evolutionary theory especially among non-scientific audiences. Here we delineate three extended instructional examples that make extensive use of NOS to establish a foundation upon which to more successfully introduce evolution. Specifically, these instructional examples enable students to consider evolutionary biology using NOS as a lens for interpretation of evolutionary concepts. We have further found, through our respective research efforts and instructional experiences, that a deep understanding of NOS helps students understand and accept the scientific validity of evolution and, conversely, that evolution provides an especially effective context for …

Yap1-Lats2 Feedback Loop Dictates Senescent Or Malignant Cell Fate To Maintain Tissue Homeostasis, Chunbo He, Xiangmin Lv, Cong Huang, Guohua Hua, Bowen Ma, Xingcheng Chen, Peter C. Angeletti, Jixin Dong, Jin Zhou, Zhengfeng Wang, Bo R. Rueda, John S. Davis, Cheng Wang

Nebraska Center for Virology: Faculty Publications

Dysfunction of the homeostasis-maintaining systems in specific cell types or tissues renders the organism susceptible to a range of diseases, including cancers. One of the emerging mechanisms for maintaining tissue homeostasis is cellular senescence. Here, we report that the Hippo pathway plays a critical role in controlling the fate of ovarian cells. Hyperactivation of Yes-associated protein 1 (YAP1), the major effector of the Hippo pathway, induces senescence in cultured primary human ovarian surface epithelial cells (hOSEs). Large tumor suppressor 2 (LATS2), the primary upstream negative regulator of YAP1, is elevated in both YAP1-induced and natural replicative-triggered senescence. Deletion of LATS2 …

After Irma, Disaster Capitalism Threatens Cultural Heritage In Barbuda, Rebecca Boger, Sophia Perdikaris

School of Global Integrative Studies: Faculty Publications

After Hurricane Irma, disaster capitalism threatens cultural heritage in Barbuda. A year and a half after the storm hit, efforts to exploit Barbuda to benefit the rich and powerful threaten to erode culture, identity, and traditional land relations in the name of “development.”

Metabolomics Of Sorghum Roots During Nitrogen Stress Reveals Compromised Metabolic Capacity For Salicylic Acid Biosynthesis, Amy M. Sheflin, Dawn Chiniquy, Chaohui Yuan, Emily Goren, Indrajit Kumar, Max Braud, Thomas Brutnell, Andrea L. Eveland, Susannah Tringe, Peng Liu, Stephen Kresovich, Ellen Marsh, Daniel P. Schachtman, Jessica E. Prenni

Nebraska Center for Biotechnology: Faculty and Staff Publications

Sorghum (Sorghum bicolor [L.] Moench) is the fifth most productive cereal crop worldwide with some hybrids having high biomass yield traits making it promising for sustainable, economical biofuel production. To maximize biofuel feedstock yields, a more complete understanding of metabolic responses to low nitrogen (N) will be useful for incorporation in crop improvement efforts. In this study, 10 diverse sorghum entries (including inbreds and hybrids) were field-grown under low and full N conditions and roots were sampled at two time points for metabolomics and 16S amplicon sequencing. Roots of plants grown under low N showed altered metabolic profiles at …

Comparison Of Different Functions To Describe Growth From Weaning To Maturity In Crossbred Beef Cattle, Madeline J. Zimmermann, Larry A. Kuehn, Matthew L. Spangler, R. Mark Thallman, Warren M. Snelling, Ronald M. Lewis

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Cow mature weight (MWT) has increased in the past 30 yr. Larger cows cost more to maintain, but their efficiency—and thus profitability— depends on the production environment. Incorporating MWT effectively into selection and mating decisions requires understanding of growth to maturity. The objective of this study was to describe growth to maturity in crossbred beef cattle using Brody, spline, and quadratic functions. Parameter estimates utilized data on crossbred cows from cycle VII and continuous sampling phases of the Germplasm Evaluation Program at the U.S. Meat Animal Research Center. The MWT were estimated at 6 yr from the fitted parameters obtained …

Spatio-Temporal Patterns And Characteristics Of Swine Shipments In The U.S. Based On Interstate Certificates Of Veterinary Inspection, Erin E. Gorsich, Ryan S. Miller, Holly M. Mask, Clayton Hallman, Katie Portacci, Colleen T. Webb

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Domestic swine production in the United States is a critical economic and food security industry, yet there is currently no large-scale quantitative assessment of swine shipments available to support risk assessments. In this study, we provide a national-level characterization of the swine industry by quantifying the demographic (i.e. age, sex) patterns, spatio-temporal patterns, and the production diversity within swine shipments. We characterize annual networks of swine shipments using a 30% stratified sample of Interstate Certificates of Veterinary Inspection (ICVI), which are required for the interstate movement of agricultural animals. We used ICVIs in 2010 and 2011 from eight states that …

Cooking Frequency Associated With Dietary Quality In Icook-4h Youth Participants At Baseline, Amber D. Ford, Sarah E. Colby, Marissa Mcelrone, Lisa Franzen-Castle, Melissa D. Olfert, Kendra K. Kattelmann, Adrienne A. White

Department of Nutrition and Health Sciences: Faculty Publications

Background: Increased intakes of ready-made and fast foods paralleled with decreased homemade food consumption have been associated with increased rates of obesity. Researchers have shown associations between cooking self-efficacy (SE) and cooking frequency (CF) with dietary quality and weight status. Some cooking interventions have shown positive associations with dietary outcomes, such as increased fruit and vegetable intake and decreased fast food consumption. There is still much unknown about SE and CF, especially among youth.

Objective: Determine baseline SE and CF and the associations with dietary quality and body mass index (BMI) of youth enrolled in iCook 4H.

Methods: Youth (n …

Autodissemination Of Pyriproxyfen As A Method For Controlling The House Fly Musca Domestica, Haim Biale, Elad Chiel, Christopher J. Geden

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

House fly (Musca domestica) control is a major challenge in animal agriculture. Here, we tested the feasibility of applying pyriproxyfen (PPF), an insect-growth regulator that controls house flies effectively, using autodissemination methods, in which the flies themselves deliver PPF to their oviposition sites. First, we tried baiting gravid female flies to walk-through stations, where flies would self-treat with PPF and distribute it. This concept worked well in laboratory and indoor cage experiments, but not in the field, as flies appeared reluctant to alight on and collect PPF. Therefore, we tested a different concept of actively coating flies with PPF and …

Micrornas In Bovine Milk Exosomes Are Bioavailable In Humans But Do Not Elicit A Robust Pro-Inflammatory Cytokine Response, Ezra Mutai, Amanda E. Ramer-Tait, Janos Zempleni

Department of Nutrition and Health Sciences: Faculty Publications

Background: Bovine milk exosomes are studied for their roles as bioactive food compounds and as vehicles for drug delivery. Both lines of investigation converge on immune function, e.g., immune regulation by absorption of microRNAs encapsulated in milk exosomes across species boundaries, and the possibility of exosomes and their cargos triggering an immune response if used in drug delivery. This study assessed the bioavailability of immune-related microRNAs from bovine milk and changes in plasma cytokine concentrations after milk consumption in humans, and the secretion of cytokines by human peripheral blood mononuclear cells (PBMCs) cultured with milk exosomes transfected with immune-relevant microRNAs. …

Environmental And Agricultural Relevance Of Humic Fractions Extracted By Alkali From Soils And Natural Waters, D. C. Olk, P. R. Bloom, E. M. Perdue, D. M. Mcknight, Y. Chen, A. Farenhorst, N. Senesi, Y.-P Chin, P. Schmitt-Kopplin, N. Hertkorn, M. Harir

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

To study the structure and function of soil organic matter, soil scientists have performed alkali extractions for soil humic acid (HA) and fulvic acid (FA) fractions for more than 200 years. Over the last few decades aquatic scientists have used similar fractions of dissolved organic matter, extracted by resin adsorption followed by alkali desorption. Critics have claimed that alkaliextractable fractions are laboratory artifacts, hence unsuitable for studying natural organic matter structure and function in field conditions. In response, this review first addresses specific conceptual concerns about humic fractions. Then we discuss several case studies in which HA and FA were …

Invasive Feral Swine Damage To Globally Imperiled Steephead Ravine Habitats And Influences From Changes In Population Control Effort, Climate, And Land Use, Richard M. Engeman, Erica Laine, John Allen, Jeremy Preston, William Pizzolato, Brett Williams, Amanda Stevens Kreider, Dennis Teague

United States Department of Agriculture Wildlife Services: Staff Publications

Steephead ravines are unusual geological features primarily occurring in Florida’s panhandle, a biodiversity hotspot. The unique habitats formed by steepheads are extremely valuable biodiversity resources within this larger area of great biodiversity. Eglin Air Force Base (EAFB) is essential for global conservation of steepheads because this vast area holds the greatest number under single ownership. Steepheads are significantly threatened by feral swine rooting damage. A decade-long investigation of EAFB’s steepheads assessed the following: (1) severity of swine damage to steepheads, (2) changing levels of swine control on swine population and damage, (3) changing climatic conditions on damage, (4) changing military …

Blood Parasites Infecting The Hoatzin (Opisthocomus Hoazin), A Unique Neotropical Folivorous Bird, M. Andreína Pacheco, M. Alexandra García-Amado, Jaime Manzano, Nubia E. Matta, Ananias A. Escalante

Harold W. Manter Laboratory of Parasitology: Library Materials

The Hoatzin (Opisthocomus hoazin) is the only extant member of the order Opisthocomiformes. This unique South American bird lives in the riparian lowland vegetation characteristic of the Amazon and Orinoco basins. Hoatzins nest in communal social units close to water bodies; they are strictly folivores being the only bird with pregastric fermentation in the crop. Because of the complex logistics involved in capturing this bird, there is a knowledge gap on its parasites. This study documents two distant lineages of haemosporidian parasites (Plasmodium spp.) in a juvenile and two adults sampled in the Cojedes state, Venezuela. Although …

Critical Role Of Post-Transcriptional Regulation For Ifn-Γ In Tumor-Infiltrating T Cells, Fiamma Salerno, Aurelie Guislain, Julian J. Freen-Van Heeren, Benoit P. Nicolet, Howard A. Young, Monika C. Wolkers

United States Public Health Resources

Protective T cell responses against tumors require the production of Interferon gamma (IFN-γ). However, tumor-infiltrating T cells (TILs) gradually lose their capacity to produce IFN-γ and therefore fail to clear malignant cells. Dissecting the underlying mechanisms that block cytokine production is thus key for improving T cell products. Here we show that although TILs express substantial levels of Ifng mRNA, post-transcriptional mechanisms impede the production of IFN-γ protein due to loss of mRNA stability. CD28 triggering, but not PD1 blocking antibodies, effectively restores the stability of Ifng mRNA. Intriguingly, TILs devoid of AU-rich elements within the 3ʹuntranslated region maintain stabilized …

Winter Field Report, December 2018 To February 2019, W. Ross Silcock

Nebraska Bird Review

As has been the trend in recent winters, and despite the relative coolness of this winter, there were numerous examples of species north of expected midwinter ranges or present in higher numbers than usual in winter. The long list includes unexpected midwinter records of Tundra Swan, American Wigeon, Ring-necked Duck, Lesser Scaup, White-winged Dove, Sandhill Crane, Clark’s Grebe, Doublecrested Cormorant, Red-shouldered Hawk, and Yellow-bellied Sapsucker. Startling was the state’s first winter record of Sedge Wren, perhaps the biggest rarity of the season. And Eastern Meadowlark was documented in midwinter for the first time, although its presence in the southeast has …

Bird Monitoring At Effigy Mounds National Monument, Iowa, Status Report 2009–2017, David G. Peitz, Lloyd W. Morrison, Kristen L. Mecke

United States National Park Service: Publications

Executive Summary

In 2009, the Heartland Inventory and Monitoring (I&M) Network) initiated breeding bird surveys on Effigy Mounds National Monument (NM), Iowa, to address two objectives: (1) to monitor changes in bird community composition and abundance, and (2) to improve our understanding of relationships between breeding birds and habitat and the effects of management actions on such relationships. This report evaluates trends in the park’s breeding bird populations in the context of trends observed within the North American Bird Conservation Initiative’s (NABCI) Prairie Hardwood Transition Bird Conservation Region, the bird conservation region in which the park is located. By doing …

Plant Community Composition And Structure Monitoring At Agate Fossil Beds National Monument, 2018 Data Report, Ryan M. Manuel

United States National Park Service: Publications

Abstract

This report presents the results of vegetation monitoring efforts in 2018 at Agate Fossil Beds National Monument (AGFO) by the Northern Great Plains Inventory and Monitoring Network (NGPN) and the Northern Great Plains Fire Effects Program (NGPFire). This was the eighth year of field work conducted by NGPN at AGFO.

In 2018, crew members from NGPN visited six long-term plant community monitoring (PCM) plots, four legacy plant community monitoring (LPCM) plots, and NGPFire visited one additional PCM plot to collect data on plant communities at AGFO. This collaborative effort is part of a long-term monitoring program established to better …