Digital Commons Network^™

Evolution Of An Institutional Repository: A Case History From Nebraska, Paul Royster

University of Nebraska-Lincoln Libraries: Faculty Publications

The 13-year history of the institutional repository (IR) at the University of Nebraska–Lincoln is recounted with emphasis on local conditions, administrative support, recruitment practices, and management philosophy. Practices included offering new services, hosting materials outside the conventional tenure stream, using student employees, and providing user analytics on global dissemination. Acquiring trust of faculty depositors enhanced recruitment and extra-library support. Evolution of policies on open access, copyright, metadata, and third-party vendors are discussed, with statistics illustrating the growth, contents, and outreach of the repository over time. A final section discusses future directions for scholarly communications and IRs in particular.

An Integrated Genomics And Phenomics Approach To Study The Evolution Of C4 Photosynthesis, Daniel Santana De Carvalho

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The C4 photosynthetic pathway was first described over 50 years ago. Today, it is known that C4 evolved independently > 60 in plant lineages, which involves understanding not only the genetic, but also the metabolic features and differences involved in this process. Also, several adaptations are involved in the evolution of this type of photosynthesis, for example: changes in leaf anatomy and the evolution of kranz anatomy, physiology and metabolic pathways. In order to further investigate this pathway, different technologies and methods have been developed to unravel genes involved in C4 photosynthesis. With the advances in molecular biology and bioinformatics tools …

Enhancing Self-Monitoring With Differential Negative Reinforcement Of Alternative Behavior For Increasing Students’ Writing Production, Meghann Torchia

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Writing is a difficult task for many students who find it aversive, and who attempt to escape the task. Self-monitoring and differential negative reinforcement of alternative behavior (DNRA) are two approaches that have been shown to improve quantity of performance, but no studies were found that combined the two methods to determine whether they are more effective in combination than in isolation. The purpose of the present study was to evaluate the effectiveness of using DNRA to enhance self-monitoring for increasing writing productivity using a multiple probe, across participants, design. Number of words and number of sentences were measured. For …

Promoting Bee Communities Through Habitat Enhancements On Public And Private Lands In Nebraska, Kayla A. Mollet

Department of Entomology: Dissertations, Theses, and Student Research

Wild and managed bees are the most effective pollinators, accounting for about 80% of the pollination of flowering plants and 75% of fruits, nuts, and vegetables in the United States (USDA, 2019; USFWS, 2019). An estimated 4,000 species of bees reside in North America, the majority of which are wild and unmanaged. Wild bee communities are critical for maintaining healthy ecosystems, as they sustain native flora that provides soil stability and habitat for other wildlife. In a changing landscape, floral enhancements on privately and publicly-owned lands may have great impact for improving habitat for pollinators across the United States. Planting …

Environmental Sampling Techniques For Herd-Level Surveillance Of Bovine Viral Diarrhea Virus, Jaden Carlson

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Control of Bovine Viral Diarrhea Virus (BVDV) relies on resource-intensive individual animal sampling to detect and remove persistently infected (PI) cattle. Herd-level surveillance tools would be useful for herds with unknown BVDV status and for monitoring herds with BVDV-free status. The overall objective of this thesis is to explore the viability of BVDV surveillance at a herd-level using samples collected without handling individual animals. The first objective was to determine the feasibility of using stable flies as a sampling tool to detect BVDV. The second objective was to determine the feasibility of using drinking water to detect BVDV. To accomplish …

3-Hydroxy Fatty Acids Induce Retinal Pigment Epithelial Cell Lipoapoptosis, Mona Hadidi

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Mitochondrial fatty acid oxidation (FAO) plays an important role in maintaining metabolic homeostasis, especially during catabolic stress. Genetic mutation in gene that encodes for mitochondrial FAO enzymes could result in metabolic stress especially during fasting or hypoglycemic condition. Mutation in the active site residue (1528G>C) of long chain 3-hydroxy acyl CoA dehydrogenase (LCHAD) results in the loss of LCHAD enzyme activity. Children’s with LCHAD mutation were known to accumulate 3-hydroxy fatty acids such as 3-hydroxy palmitic acid and 3-hydroxy myristic acids, long chain free fatty acids, acyl carnitine and 3-hydroxy dicarboxylic acid due to their defective enzyme activity. These …

Inhibitory Effects Of Shiitake-Derived Exosome-Like Nanoparticles On Nlrp3 Inflammasome Activation, Yizhu Lu

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

The NLRP3 inflammasome is a critical mediator of inflammation and consists of the sensor NOD-like receptor family, pyrin domain containing 3 (NLRP3), the adaptor apoptotic speck protein containing a caspase recruitment domain (ASC), and the effector caspase-1. Dysregulated or excessive activation of the NLRP3 inflammasome contributes to pathogenesis of diverse inflammatory diseases such as Type 2 diabetes and atherosclerosis. Therefore, the NLRP3 inflammasome is a promising therapeutic target for treating these diseases. Extracellular vesicles (EVs) are membrane-enclosed tiny particles released by almost any type of cells and they are involved in the intercellular communication by transferring their cargos including RNAs, …

Transport And Distribution Of Bovine Milk Exosomes And Mir-34a Cargo In Murine Cerebral Cortex Endothelial Bend.3 Cells And Bv2 Microglia, Pearl Ebea

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

The blood-brain barrier (BBB) poses an obstacle in the delivery of drugs to the brain. Bovine milk exosomes (BME) are explored for delivering antisense oligonucleotides to tumors, because BME are bioavailable and protect RNA cargos against degradation in the gastrointestinal tract. This study had the following objectives: 1) assess the transport kinetics of BME and their RNA cargos and secretion of RNA across the apical membrane in murine cerebral cortex endothelial bEnd.3 cells and 2) determine whether murine brain BV2 microglia have the potential to accumulate and, therefore, eliminate BME that crossed the BBB. The uptake of BME labeled with …

Cooperative Learning For The Consensus Of Multi-Agent Systems, Qishuai Liu

Department of Electrical and Computer Engineering: Dissertations, Theses, and Student Research

Due to a lot of attention for the multi-agent system in recent years, the consensus algorithm gained immense popularity for building fault-tolerant systems in system and control theory. Generally, the consensus algorithm drives the swarm of agents to work as a coherent group that can reach an agreement regarding a certain quantity of interest, which depends on the state of all agents themselves. The most common consensus algorithm is the average consensus, the final consensus value of which is equal to the average of the initial values. If we want the agents to find the best area of the particular …

Influence Of Stem Lessons On Critical Thinking, Brooke Waddell

Department of Teaching, Learning, and Teacher Education: Theses and Other Student Research

The study of science, technology, engineering, and math (STEM) is leading educators into a new world of teaching. The classroom roles have been reversed and students are now in charge of their own learning. Students are learning how to engineer and solve real-world problems through critical thinking. Integrated STEM lessons are teaching students to use their prior knowledge across subject areas to prepare themselves for the workforce needed in the 21^st century. This study was conducted in a rural second grade classroom with the number of students ranging from 16 to 21. Throughout this study qualitative data was collected. …

Which Credence Production Attributes Do Consumers Prefer? The Case Of Milk, Emmanouil Petrakis

Department of Agricultural Economics: Dissertations, Theses, and Student Research

The interest of consumers for non-traditional food production attributes like antibiotics free and animal welfare have made consumer preferences more complex. These new attributes, usually non-identifiable by inspection or experience of the product are called credence attributes. The objectives of this study are to analyze consumer preferences for milk production attributes by eliciting the relative importance of these attributes for consumers, and to identify possible sources of heterogeneity related to consumer characteristics and actual purchase data. The research method used to elicit consumer preferences is Paired Comparison Method. Consumers from California and Texas were administered the online survey questionnaire in …

How Does Identifying As Gluten-Free Impact Information Choice Regarding The Gluten-Free Diet?, Pratiksha Baishya

Department of Agricultural Economics: Dissertations, Theses, and Student Research

The market for gluten-free products is a multi-billion-dollar industry in the United States and has seen tremendous growth in the recent years. The retail sales of gluten-free foods in the United States almost tripled between 2011 and 2015, although rates of diagnosed gluten-related health problems have not risen. In addition to people who suffer from Celiac Disease, Wheat Allergy and Non-Celiac Gluten Sensitivity, there is a category or people known as PWAG (people who avoid gluten) who seem to have significantly contributed to this boom in the market for gluten-free foods. With more people choosing to adopt the gluten-free diet, …

Dual Biological Control: Characterization Of Fungi And Bacteria To Control Granary Weevil And Fungal Pathogens Of Stored Grain, Gülçin Ercan

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Cereals are main food sources for humans and animals. However, during storage, cereal grains can be infested by insects and fungi. One of the most important insect storage pests is Sitophilus granarius (L., Coleoptera: Curculionidae). Adults and larvae can cause serious grain losses. In addition to insect pests, fungal pathogens may also invade the grain and cause economic loss, including contamination with mycotoxins, which threaten mammal health by causing serious disease. The most common mycotoxigenic grain fungi are species that belong to the genera Fusarium, Aspergillus and Penicillium. Currently, the most commonly used management strategies for insect and …

The Value Of Inspection Stations For Detecting Nonindigenous Species Lacking Agricultural Significance: The Mexican Treefrog, Smilisca Baudinii Complex (Duméril And Bibron 1841) (Hylidae), Interdicted In Florida, Usa, From A Shipment Of Peppers, Louis A. Somma

Papers in Herpetology

A Mexican Treefrog, Smilisca baudinii, a nonindigenous species, was interdicted for the first time from an imported shipment of peppers. The value of agriculture inspection stations used to make these interdictions is discussed. This is a single cargo interception (Stage 1: Colautti and MacIsaac 2004) and the first record for S. baudinii intercepted in Florida. Currently no evidence suggests that S. baudinii has been successfully introduced into and established in Florida, although this species could survive climatic conditions in the southern part of the state and at the scheduled destination of this shipment.

Cargo transport of alien species is a …

Effects Of Alternative Carbohydrate Sources And Soybean-Derived Isoflavones On The Microbiome And Immune System Of Nursery Piglets And Intestinal Porcine Epithelial Cell Line J-2, Sydney Kinstler

Department of Animal Science: Dissertations, Theses, and Student Research

Four experiments were conducted to characterize the microbiome of the piglet and observe effects of diet on growth performance and immunity. In Exp. 1, 96 nursery piglets were fed varying carbohydrate sources and in Exp. 2, 64 piglets were fed a soy protein concentrate or soybean meal as-is or supplemented with an isoflavone concentrate. Body and feeder weights were measured for growth performance estimation. Luminal contents and scrapings were isolated for microbial composition analysis. Fecal and blood samples were extracted in Exp. 2 for microbiome and immunity analysis. Diets did not affect growth performance (P > 0.10); however, Exp. 1 …

Evaluation Of Alpha Amylase Containing Corn On Beef Cattle Performance And Digestibility And Double-Cropped Annual Forages Following Corn Harvest, Mckenna Brinton

Department of Animal Science: Dissertations, Theses, and Student Research

One digestion and three feedlot trials evaluated the effect of a new corn hybrid containing an α-amylase enzyme trait, Syngenta Enogen Feed Corn (SYT-EFC) on site and extent of digestion, ruminal fermentation parameters, and feedlot performance. Experiments utilized corn containing the enzymatic gene (SYT-EFC) compared to commercially available corn (CON), processed as corn silage (CS) kernel processed or not (KP), dry-rolled corn (DRC), high-moisture corn (HMC), or a blend of DRC and HMC. Growing calves fed high inclusions of CS, displayed increased G:F when CS was KP, resulting in a 6.5% improvement in G:F. Hybrid and kernel processing did not …

Validation Of A Death Assay For Angiostrongylus Cantonensis Larvae (L3) Using Propidium Iodide In A Rat Model (Rattus Norvegicus), Susan I. Jarvi, John Jacob, Robert T. Sugihara, Israel L. Leinbach, Ina H. Klasner, Lisa M. Kaluna, Kirsten A. Snook, M. Kathleen Howe, Steven H. Jacquier, Ingo Lange, Abigail L. Atkinson, Ashley R. Deane, Chris N. Niebuhr, Shane R. Siers

United States Department of Agriculture Wildlife Services: Staff Publications

Angiostrongylus cantonensis is a pathogenic nematode and the cause of neuroangiostrongyliasis, an eosinophilic meningitis more commonly known as rat lungworm disease. Transmission is thought to be primarily due to ingestion of infective third stage larvae (L3) in gastropods, on produce, or in contaminated water. The gold standard to determine the effects of physical and chemical treatments on the infectivity of A. cantonensis L3 larvae is to infect rodents with treated L3 larvae and monitor for infection, but animal studies are laborious and expensive and also raise ethical concerns. This study demonstrates propidium iodide (PI) to be a reliable marker of …

The Value Of Grazed Corn Residue For Crop And Cattle Producers, Jay Parsons, D. D. Redfearn, Mary E. Drewnoski

Cornhusker Economics

The Western Corn Belt has historically been corn and wheat cropping systems with cattle integrated on nearby grasslands. Recent agricultural production data ranked Nebraska, South Dakota, Kansas, and North Dakota in the top 10 states for both corn and beef cattle production in the United States. In 2017, these four states had about 20% of the beef cow inventory in the United States with Nebraska alone having 1.9 million beef cows (USDA National Agricultural Statistics Service, 2018). Forage-based livestock production is a fundamental component of these agricultural economies. However, a large quantity of grasslands in this region were converted into …

High Pressure Thawing Of Raw Poultry Meats, Ali Alqaraghuli

Department of Food Science and Technology: Dissertations, Theses, and Student Research

The melting temperature of water reaches its minimum when pressurized from 0 °C at atmospheric pressure down to -22 °C at 220 MPa, showing that frozen products at atmospheric pressure (0.1 MPa) can be thawed rapidly by simply increasing the pressure to 210-250 MPa. High pressure thawing (HPT) of poultry meats (ground chicken, chicken breast, thigh, liver, gizzard, and heart) commonly used in the raw pet food industry was evaluated at 240 MPa, two processing fluid temperatures (refrigerated, 0-10 °C and room, 20-30 °C), and four holding times (1, 180, 360, and 540 s). Changes in color via * * …

Changes In Mammalian Abundance Through The Eocene–Oligocene Climate Transition In The White River Group Of Nebraska, Usa, Robert Gillham

Department of Earth and Atmospheric Sciences: Dissertations, Theses, and Student Research

Marine records show major cooling during the Eocene-Oligocene Climate Transition (EOCT). Most proxy studies in the White River Group suggest drying across the EOCT, and some suggest cooling. The lower resolution continental record has hindered a direct correlation of the marine climate record to Nebraska. I explore various correlation schemes and what they imply for faunal changes. This study compiles and analyzes data from 4,875 specimens in the University of Nebraska State Museum (UNSM) collection to test the hypothesis that climate change across the Eocene-Oligocene (E-O) boundary caused significant abundance changes in mammals. A series of binning schemes was created. …

Type X Strains Of Toxoplasma Gondii Are Virulent For Southern Sea Otters (Enhydra Lutris Nereis) And Present In Felids From Nearby Watersheds, Karen Shapiro, Elizabeth Vanwormer, Andrea Packham, Erin Dodd, Patricia A. Conrad, Melissa Miller

School of Veterinary and Biomedical Sciences: Faculty Publications

Why some Toxoplasma gondii-infected southern sea otters (Enhydra lutris nereis) develop fatal toxoplasmosis while others have incidental or mild chronic infections has long puzzled the scientific community. We assessed robust datasets on T. gondii molecular characterization in relation to detailed necropsy and histopathology results to evaluate whether parasite genotype influences pathological outcomes in sea otters that stranded along the central California coast. Genotypes isolated from sea otters were also compared with T. gondii strains circulating in felids from nearby coastal regions to assess land-to-sea parasite transmission. The predominant T. gondii genotypes isolated from 135 necropsied sea otters were atypical Type …

Reproductive Ecology And Movement Of Pallid Sturgeon In The Upper Missouri River, Montana, Luke M. Holmquist, Christopher S. Guy, Anne Tews, David J. Trimpe, Molly A.H. Webb

United States Fish and Wildlife Service: Publications

Successful recruitment of endangered pallid sturgeon has not been documented in the upper Missouri River basin for decades, and research on the reproductive ecology of pallid sturgeon has been hindered by low sample size. A conservation propagation program was initiated in the 1990s, and the oldest age class of hatchery‐origin pallid sturgeon are becoming sexually mature increasing the number of reproductively‐ active fish in the system. However, it is currently unknown how the reproductive ecology of hatchery‐origin pallid sturgeon relates to the few remaining wild fish. Following spring reproductive assessments, weekly relocations were recorded for each individual from late‐May to …

Contact Rates With Nesting Birds Before And After Invasive Snake Removal: Estimating The Effects Of Trap-Based Control, Amy A. Yackel Adams, Melia G. Nafus, Page E. Klug, Björn Lardner, M.J. Mazurek, Julie A. Savidge, Robert N. Reed

United States Department of Agriculture Wildlife Services: Staff Publications

Invasive predators are responsible for almost 60% of all vertebrate extinctions worldwide with the most vulnerable faunas occurring on islands. The brown treesnake (Boiga irregularis) is a notorious invasive predator that caused the extirpation or extinction of most native forest birds on Guam. The success of avian reintroduction efforts on Guam will depend on whether snake-control techniques sufficiently reduce contact rates between brown treesnakes and reintroduced birds. Mouse-lure traps can successfully reduce brown treesnake populations at local scales. Over a 22-week period both with and without active snake removal, we evaluated snake-trap contact rates for mouse- and bird-lure traps. Bird-lure …

Osl And Ceramic Analysis At The Humphrey Site, Ryan Mathison

Department of Anthropology: Theses and Student Research

The Sand Hills of Nebraska are a unique environment located in the west-central portion of Nebraska. This portion of North America has long supported human life. One group in particular that called the Sand Hills home are the Dismal River people. Dismal River is the name that archaeologists gave to a group of horticulturalists that lived in circular structures on the sand dunes, often near the rivers, in the Sand Hills. This group, while generally known through archaeology, also has a potential historic or ethnographic presence in the form of the Cuartalejo Apache visited by Ulibarri, and potentially mentioned by …

An Evaluation Of The Registration And Use Prospects For Four Candidate Toxicants For Controlling Invasive Mongooses (Herpestes Javanicus Auropunctatus), Emily W. Ruell, Chris N. Niebuhr, Robert T. Sugihara, Shane R. Siers

United States Department of Agriculture Wildlife Services: Staff Publications

The eradication or control of invasive small Indian mongooses from islands likely requires toxic baiting when removal by trapping proves insufficient. The one toxic bait currently registered for mongooses in the United States has relatively low palatability and efficacy for mongooses. Developing and registering a new pesticide can be very expensive, while funding for developing toxicants for mongooses is limited. Once registered, use of a toxic bait may be hindered by other factors, such as public opposition to an inhumane toxicant, poorer efficacy than expected, or if the toxic bait is difficult for applicators to apply or store. Therefore, we …

Network‐Based Feature Selection Reveals Substructures Of Gene Modules Responding To Salt Stress In Rice, Qian Du, Malachy Campbell, Huihui Yu, Kan Liu, Harkamal Walia, Qi Zhang, Chi Zhang

Center for Plant Science Innovation: Faculty Publications

Rice, an important food resource, is highly sensitive to salt stress, which is directly related to food security. Although many studies have identified physiological mechanisms that confer tolerance to the osmotic effects of salinity, the link between rice genotype and salt tolerance is not very clear yet. Association of gene co‐expression network and rice phenotypic data under stress has penitential to identify stress‐responsive genes, but there is no standard method to associate stress phenotype with gene co‐expression network. A novel method for integration of gene co‐expression network and stress phenotype data was developed to conduct a system analysis to link …

How Characteristic Is The Species Characteristic Selection Scale?, Erica F. Stuber, Joseph J. Fontaine

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Aim: The importance of framing investigations of organism–environment relationships to interpret patterns at relevant spatial scales is increasingly recognized. However, most research related to environmental relationships is single‐scaled, implicitly or explicitly assuming that a “species characteristic selection scale” exists. We tested the premise that a single characteristic scale exists to understand species– environment relationships within species by asking (a) what are the characteristic scales of species’ relationships with environmental predictors, and (b) is within species, cross‐predictor consistency in characteristic scales a general phenomenon.

Location: Nebraska, USA.

Time period: 2016.

Major taxa studied: Birds.

Methods: We used data from 86 species …

Understanding Preservice Teachers' Spatial Reasoning And How It Affects Their Work With Elementary Students, Michelle R. Metzger,

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Spatial reasoning involves those skills that allow one to mentally picture and manipulate objects which plays a unique role in learning and succeeding in science, technology, engineering, and mathematics fields (STEM). Despite the urgent need for strong spatial reasoning skills, our current education system spends little time fostering elementary students’ visual and spatial reasoning skills. This is becoming increasingly problematic as the need to become literate in the STEM fields has never been greater.

The purpose of this qualitative study was to examine the spatial reasoning skills that preservice teachers demonstrated and how their spatial reasoning skills were used in …

The Relationship Between Socioeconomic Status And White Matter Microstructure In Pre-Reading Children: A Longitudinal Investigation, Ola Ozernov-Palchik, Elizabeth S. Norton, Yingying Wang, Sara D. Beach, Jennifer Zuk, Maryanne Wolf, John D.E. Gabrieli, Nadine Gaab

Center for Brain, Biology, and Behavior: Faculty Publications

Reading is a learned skill crucial for educational attainment. Children from families of lower socioeconomic status (SES) tend to have poorer reading performance and this gap widens across years of schooling. Reading relies on the orchestration of multiple neural systems integrated via specific white-matter pathways, but there is limited understanding about whether these pathways relate differentially to reading performance depending on SES background. Kindergarten white-matter FA and second-grade reading outcomes were investigated in an SES-diverse sample of 125 children. The three left-hemisphere white-matter tracts most associated with reading, and their right-hemisphere homologs, were examined: arcuate fasciculus (AF), superior longitudinal fasciculus …

Operationalizing Ecological Resilience Concepts For Managing Species And Ecosystems At Risk, Jeanne C. Chambers, Craig R. Allen, Samuel A. Cushman

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

This review provides an overview and integration of the use of resilience concepts to guide natural resources management actions. We emphasize ecosystems and landscapes and provide examples of the use of these concepts from empirical research in applied ecology. We begin with a discussion of definitions and concepts of ecological resilience and related terms that are applicable to management. We suggest that a resilience-based framework for management facilitates regional planning by providing the ability to locate management actions where they will have the greatest benefits and determine effective management strategies. We review the six key components of a resilience-based framework, …