Digital Commons Network^™

Differential Longitudinal Establishment Of Human Fecal Bacterial Communities In Germ-Free Porcine And Murine Models, Nirosh Aluthge, Wesley A. Tom, Alison Bartenslager, Thomas E. Burkey, Phillip S. Miller, Kelly D. Heath, Craig Kreikemeier-Bower, Hatem Kittana, Robert Schmaltz, Amanda Ramer-Tait, Samodha Fernando

Department of Animal Science: Faculty Publications

The majority of microbiome studies focused on understanding mechanistic relationships between the host and the microbiota have used mice and other rodents as the model of choice. However, the domestic pig is a relevant model that is currently underutilized for human microbiome investigations. In this study, we performed a direct comparison of the engraftment of fecal bacterial communities from human donors between human microbiotaassociated (HMA) piglet and mouse models under identical dietary conditions. Analysis of 16S rRNA genes using amplicon sequence variants (ASVs) revealed that with the exception of early microbiota from infants, the more mature microbiotas tested established better …

Age Related Extracellular Matrix And Interstitial Cell Phenotype In Pulmonary Valves, Shaohua Wu, Vikas Kumar, Peng Xiao, Mitchell Kuss, Jung Yul Lim, Chittibabu Guda, Jonathan Butcher, Bin Duan

Department of Animal Science: Faculty Publications

Heart valve disease is a common manifestation of cardiovascular disease and is a significant cause of cardiovascular morbidity and mortality worldwide. The pulmonary valve (PV) is of primary concern because of its involvement in common congenital heart defects, and the PV is usually the site for prosthetic replacement following a Ross operation. Although effects of age on valve matrix components and mechanical properties for aortic and mitral valves have been studied, very little is known about the age-related alterations that occur in the PV. In this study, we isolated PV leaflets from porcine hearts in different age groups (~ 4–6 …

Rod-Shape Theranostic Nanoparticles Facilitate Antiretroviral Drug Biodistribution And Activity In Human Immunodeficiency Virus Susceptible Cells And Tissues, Bhavesh D. Kevadiya, Brendan Ottemann, Insiya Z. Mukadam, Laura Castellanos, Kristen Sikora, James R. Hilaire, Jatin Machhi, Jonathan Herskovitz, Dhruvkumar Soni, Mahmudul Hasan, Wenting Zhang, Anandakumar Sarella, Jered Garrison, Joellyn Mcmillan, Benson Edagwa, R. Lee Mosley, Richard W. Vachet, Howard E. Gendelman

Nebraska Center for Materials and Nanoscience: Faculty Publications

Human immunodeficiency virus theranostics facilitates the development of long acting (LA) antiretroviral drugs (ARVs) by defining drug-particle cell depots. Optimal drug formulations are made possible based on precise particle composition, structure, shape and size. Through the creation of rod-shaped particles of defined sizes reflective of native LA drugs, theranostic probes can be deployed to measure particle-cell and tissue biodistribution, antiretroviral activities and drug retention.

Methods: Herein, we created multimodal rilpivirine (RPV) ¹⁷⁷lutetium labeled bismuth sulfide nanorods (¹⁷⁷LuBSNRs) then evaluated their structure, morphology, configuration, chemical composition, biological responses and adverse reactions. Particle biodistribution was analyzed by single …

Svat4: A Computer Program For Visualization And Analysis Of Crystal Structures, Xingzhong Li

Nebraska Center for Materials and Nanoscience: Faculty Publications

SVAT4 is a computer program for interactive visualization of three-dimensional crystal structures, including chemical bonds and magnetic moments. A wide range of functions, e.g. revealing atomic layers and polyhedral clusters, are available for further structural analysis. Atomic sizes, colors, appearance, view directions and view modes (orthographic or perspective views) are adjustable. Customized work for the visualization and analysis can be saved and then reloaded. SVAT4 provides a template to simplify the process of preparation of a new data file. SVAT4 can generate high-quality images for publication and animations for presentations. The usability of SVAT4 is broadened by a software suite …

A Global Database Of Soil Nematode Abundance And Functional Group Composition, Johan Van Den Hoogen, Stefan Geisen, Thomas W. Crowther, Peter Mullin, Many Co-Authors

Department of Plant Pathology: Faculty Publications

As the most abundant animals on earth, nematodes are a dominant component of the soil community. They play critical roles in regulating biogeochemical cycles and vegetation dynamics within and across landscapes and are an indicator of soil biological activity. Here, we present a comprehensive global dataset of soil nematode abundance and functional group composition. This dataset includes 6,825 georeferenced soil samples from all continents and biomes. For geospatial mapping purposes these samples are aggregated into 1,933 unique 1-km pixels, each of which is linked to 73 global environmental covariate data layers. Altogether, this dataset can help to gain insight into …

Phenotypic Divergence In Two Sibling Species Of Shorebird: Common Snipe And Wilson’S Snipe (Charadriiformes: Scolopacidae), Tiago M. Rodrigues, Edward H. Miller, Sergei V. Drovetski, Robert M. Zink, Jon Fjeldså, David Gonçalves

School of Biological Sciences: Faculty Publications

Natural and social selection are among the main shapers of biological diversity but their relative importance in divergence remains understudied. Additionally, although neutral evolutionary processes may promote phenotypic divergence, their potential contribution in speciation is often overlooked in studies of comparative morphology. In this study, we investigated phenotypic differentiation in two allopatric shorebirds: the Palaearctic Common Snipe Gallinago gallinago and the Nearctic Wilson’s Snipe Gallinago delicata. Specimens of Common Snipe (n = 355 skins, n = 163 skeletons) and Wilson’s Snipe (n = 403 skins, n = 141 skeletons) in natural history collections were examined to quantify differences in skeletal …

Comparative Genome Analysis Of Bifidobacterium Longum Subsp. Infantis Strains Reveals Variation In Human Milk Oligosaccharide Utilization Genes Among Commercial Probiotics, Rebbeca M. Duar, Giorgio Casaburi, Ryan D. Mitchell, Lindsey N.C. Scofield, Camila A. Ortega Ramirez, Daniela Barile, Bethany M. Henrick, Steven A. Frese

Department of Food Science and Technology: Faculty Publications

Dysbiosis is associated with acute and long-term consequences for neonates. Probiotics can be effective in limiting the growth of bacteria associated with dysbiosis and promoting the healthy development of the infant microbiome. Given its adaptation to the infant gut, and promising data from animal and in vitro models, Bifidobacterium longum subsp. infantis is an attractive candidate for use in infant probiotics. However, strain-level differences in the ability of commercialized strains to utilize human milk oligosaccharides (HMOs) may have implications in the performance of strains in the infant gut. In this study, we characterized twelve B. infantis probiotic strains and identified …

Uncoverings: The Research Papers Of The American Quilt Study Group, Volume 41 (2020), Joanna E. Evans, Deanna Allred, Linda Baumgarten, Hallie Bond, Mary Bywater Cross, Gretchen Guidess, Kimberley Smith Ivey, Charlene Bongiorno Stephens, William G. Stephens, Rosa Thornley

Uncoverings Journal

Preface by Joanna E. Evans

Unraveling the Mysteries of a White Quilt One Quilt, Two Cultures, and ­ree Approaches by Linda Baumgarten, Gretchen Guidess, and Kimberly Ivey

“Nothing Thou Love Be Lost or Die”: An 1846 Friendship Quilt Made at the Vermont Asylum for the Insane by Charlene Bongiorno Stephens and William G. Stephens

Seeking the Evidence: ­The Solar System Quilt and Artist Ellen Harding Baker by Mary Bywater Cross

“For Top-Cover Warmth” North Country Comforters, 1800–1970s by Hallie E. Bond

“Reach Out and Touch Someone”: Examining How Instagram Has Become a Conduit for Quiltmaking Communities by Deanna Allred and …

Larkspur Poisoning Of Cattle: Plant And Animal Factors That Influence Plant Toxicity, Benedict T. Green, Dale R. Gardner, Clint A. Stonecipher, Stephen T. Lee, James A. Pfister, Kevin D. Welch, Daniel Cook, T. Zane Davis, Bryan L. Strgelmeier

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Toxic larkspurs (Delphinium species) poison and kill cattle in western North America, and cattle deaths from larkspur poisoning can be as high as 15%.¹ Millions of dollars are lost from ranching communities due to animal deaths, increased management costs, and the underutilization of nutritious rangelands. Economic costs of larkspur poisoning to ranchers is not only associated with cow deaths, but, for example, other costs include loss of that cow nursing a healthy calf. The rancher must keep the calf alive, and if it survives there is lost income from marketing an early weaned, poor-performing calf. Ranchers must also replace …

Genetic Diversity Among Mycobacterium Avium Subspecies Revealed By Analysis Of Complete Genome Sequences, John Bannantine, Cyril Conde, Darrell O. Bayles, Maxime Branger, Franck Biet

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Mycobacterium avium comprises four subspecies that contain both human and veterinary pathogens. At the inception of this study, twenty-eight M. avium genomes had been annotated as RefSeq genomes, facilitating direct comparisons. These genomes represent strains from around the world and provided a unique opportunity to examine genome dynamics in this species. Each genome was confirmed to be classified correctly based on SNP genotyping, nucleotide identity and presence/absence of repetitive elements or other typing methods. The Mycobacterium avium subspeciesparatuberculosis (Map) genome size and organization was remarkably consistent, averaging 4.8 Mb with a variance of only 29.6 kb among the 13 strains. …

Plin-Amir, A Pre-Microrna-Based Technology For Controlling Herbivorous Insect Pests, Julia Bally, Elane Fishilevich, Rachel L. Doran, Karen Lee, Samanta Bolzan De Campos, Marcelo A. German, Kenneth E. Narva, Peter M. Waterhouse

Department of Entomology: Faculty Publications

The cotton bollworm, Helicoverpa armigera, is a major insect pest for a wide range of agricultural crops. It causes significant yield loss through feeding damage and by increasing the crop’s vulnerability to bacterial and fungal infections. Although expression of Bacillus thuringiensis (Bt) toxins in transgenic crops has been very successful in protecting against insect pests, including H. armigera, field-evolved resistance has occurred in multiple species. To manage resistant populations, new protection strategies must be continuously developed. Trans-kingdom RNA interference (TK-RNAi) is a promising method for controlling herbivorous pests. TK-RNAi is based on delivering dsRNA or hairpin RNA containing essential insect …

Do The Quality And Quantity Of Honey Bee-Collected Pollen Vary Across An Agricultural Land-Use Gradient?, Michael P. Simanonok, Clint R. V. Otto, Matthew D. Smart

Department of Entomology: Faculty Publications

Pollen is the source of protein for most bee species, yet the quality and quantity of pollen is variable across landscapes and growing seasons. Understanding the role of landscapes in providing nutritious forage to bees is important for pollinator health, particularly in areas undergoing significant land-use change such as in the Northern Great Plains (NGP) region of the United States where grasslands are being converted to row crops. We investigated how the quality and quantity of pollen collected by honey bees (Apis mellifera L. [Hymenoptera: Apidae]) changed with land use and across the growing season by sampling bee-collected pollen …

The Public School As The Preeminent Site Of Constitutional Law, Justin Driver

Nebraska Law Review

This speech, delivered as the Lane Lecture in Lincoln, Nebraska, on Friday, November 8, 2019, is drawn from the book The Schoolhouse Gate: Public Education, the Supreme Court, and the Battle for the American Mind, which Pantheon Books published in 2018. This book examines the intersection of two distinctively American institutions: the public school and the Supreme Court. The United States has long exhibited an uncommonly strong belief in the importance of public education and its centrality to national identity. As Adlai Stevenson once remarked, “The free common school system is the most American thing about America.” But many …

Job Training Mythologies: Stitching Up Labor Markets, Henry H. Perritt Jr.

Nebraska Law Review

I. Introduction

II. Creative Destruction

III. The Failure of Government “Adjustment Assistance” ... A. Components of Training ... 1. Elements of Training ... 2. Tools for Training ... 3. Economics of Training ... 4. Institutions for Training ... 5. Barriers to Private Sector Skills Training ... B. Weaknesses of Existing Programs ... 1. Janesville, WI: A Case Study ... 2. Registered Apprenticeships Are Not the Answer ... C. Labor Department Proposed Rule for Industry-Recognized Apprenticeships

IV. Textile Industry: A Case Study ... A. Mechanizing the Basics: Teaching in Surrogate Families (1800–1840) … B. The Speedup and Stretchout—Rings and Draper Looms: …

Masthead And Table Of Contents

Nebraska Law Review

No abstract provided.

Water And Power In Architecture: Charleston Sc, Charles Dowd

Masters in Architecture Program: Theses

Charleston has not always been the peninsula it is today. In fact over time it has increased in size. It took a tremendous leap forward between 1670 and 1802. The city has been gaining ground through the use of landfill techniques.

This project explores the relationship of ground and power. Through examining Charleston’s role in the slave trade it is revealed that expansion of the city is influenced by the political and economic values of the time and vice versa.

Under the Supervision of Professor John Craig Babe

Intelligent Design And Darwinian Evolution, Raul Sanchez

Nebraska College Preparatory Academy: Senior Capstone Projects

Darwinian Evolution has many fundamental flaws that are only being discussed in the technical papers and yet it’s portrayed as an unequivocal theory. Intelligent Design is being censored In Academia and represented poorly in public media. Even with leading scientists at the forefront of discovery and logical reasoning Intelligent Design is portrayed as a religious way of infiltrating the science classroom but in reality its far from it. Ultimately it comes down to what the evidence points to and if it points to God then let it be so.

How Animal Abuse Feeds Into Acts Of Human Violence, Elisa Soto

Nebraska College Preparatory Academy: Senior Capstone Projects

Copyright (c) 2020 by the author.

Mitogenome Of Northern Long-Eared Bat, Sarah Gaughan, Kevin L. Pope, Jeremy A. White, Cliff Lemen, Patricia Freeman

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

The complete mitogenome of the northern long-eared bat (Myotis septentrionalis) was determined to be 17,362 bp and contained 22 tRNA genes, 2 rRNA genes and one control region. The whole genome base composition was 33.8% GC. Phylogenetic analysis suggests that M. septentrionalis be positioned next to M. auriculus in the Nearctic subclade of the Myotis genus. This complete mitochondrial genome provides essential molecular markers for resolving phylogeny and future conservation efforts.

Use And Expenditures On Public Access Hunting Lands, Lyndsie S. Wszola, Lutz F. Gruber, Erica F. Stuber, Lindsey N. Messinger, Christopher J. Chizinski, Joseph J. Fontaine

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

The recreational and economic benefits of hunting on traditional permanent public lands are well-established. Increasingly popular “open fields” hunting access programs temporarily open private lands to public hunting through public-private partnerships. Open fields programs have the potential to create public hunting opportunities and economic development in rural communities, but the extent to which open fields programs compare to traditional public lands at providing benefits to hunters and rural communities has not yet been evaluated. We compared hunter use and expenditures on open fields lands and traditional public lands in Nebraska, USA. We used Convolution Likelihood Ecological Abundance Regression, a novel …

Exit Here: Strategies For Dealing With Aging Dams And Reservoirs, Henry H. Hansen, Emily Forzono, Alisha Grams, Lindsay Ohlman, Christine Ruskamp, Mark A. Pegg, Kevin L. Pope

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Aging infrastructure is prevalent throughout the world, but water control management structures, specifically dams, are of growing concern. Dams and their corresponding reservoirs have inherent, but separate, lifespans. The proportion of dams around the world that continue operation beyond their intended lifespans is growing at an alarming rate. Society will not only have to navigate the tradeoffs associated with the deterioration of services provided by reservoirs and dams, but also impending structural failures. Society is nearing a critical pinch point where we will have to decide how to deal with dams and reservoirs at scales that range from a single …

Learning Science With Mobile Technologies: Opportunities For Enhancing Preservice Elementary Teachers’ Science Conceptual Understanding, Deepika Menon, Zarah Salas, Allison Mellendick, Meera Chandrasekhar, Dorina Kosztin

Department of Teaching, Learning, and Teacher Education: Faculty Publications

The use of technology is increasing rapidly in our society, and classroom teachers must recognize the impact and importance of technology in the lives of their students. It is crucial that college faculty involved in teacher training design courses that integrate mobile technologies to prepare the next generation of teachers. In this study, we investigate the effectiveness of an iPadbased curriculum app, Exploring Physics, to enhance preservice elementary teachers’ physics conceptual understanding in a physical science content course. Data were collected using a pre-and postPhysics Conceptual Understanding (PCU) survey and open-ended questionnaires. We found significant statistical gains in participants’ (N …

2020 Annual Report, Nebraska Game And Parks

Nebraska Game and Parks Commission: Publications

No abstract provided.

Threatened And Endangered Species Listing/Delisting Recommendations

Nebraska Game and Parks Commission: Publications

The Nebraska Game and Parks Commission has the statutory responsibility of administering Nebraska’s Nongame and Endangered Species Conservation Act. One of the Commission’s duties under the Act is to determine which species are endangered or threatened in the state. According to Nebraska Revised Statute § 37-806, determinations regarding endangered and threatened species shall be made on the basis of the best scientific, commercial, and other data available to the Commission. Revisions to the list of endangered and threatened species were last completed in 2000, and since then much new information was gained regarding the abundance, distribution, and imperilment status of …

Sharp‐Tailed Grouse In The Nebraska Sandhills Select Residual Cover Patches For Nest Sites, William L. Vodehnal, Gregory L. Schenbeck, Daniel W, Uresk

Nebraska Game and Parks Commission: Staff Research Publications

We evaluated selection and availability of residual cover (dead standing herbage) by sharptailed grouse (Tympanuchus phasianellus) at time of nest‐site selection in an intact and annually grazed grassland. We used radiotelemetry in 1988–1990 to locate 147 nests in the sandhills of Nebraska, USA, and classified 121 as initial nests and 26 as renests. We used visual obstruction readings (VOR) to measure the height and density of residual cover at nests and 373 landscape‐scale transects around leks (trap sites). We excluded 77 nests from vegetation analysis because green herbage or early livestock grazing compromised residual cover measurements. Most females …

Obituary: Thomas Henry Kunz (1938–2020), Allen Kurta, Winifred F. Frick, M. Brock Fenton, Polly Campbell, Gary F. Mccracken, Robert M. Timm, Hugh H. Genoways

University of Nebraska State Museum: Mammalogy Papers

Dr. Thomas Henry Kunz, an internationally recognized expert on the ecology and behavior of bats and Professor at Boston University, passed away on April 13, 2020 in Dedham, Massachusetts, at the age of 81 as the result of complications from COVID-19. “Tom,” to his many friends and colleagues, was born on June 11, 1938 in Independence, Missouri, to William H. and Edna F. (Dornfeld) Kunz. He married Margaret Louise Brown on December 27, 1962 in Faucett, Missouri, Margaret’s hometown. Two children were born to Margaret and Tom—Pamela Kunz (Jeffrey Kwan) and David Kunz (Nicole, née D’Angelo), and five grandchildren.

As …

Nefdc Exchange, Volume 33, Special Edition 2020, New England Faculty Development Consortium

New England Faculty Development Consortium Exchange

Contents

Notes from the guest editor, Eric Matte (Landmark College)

Rescuing the canary in the coal mine: Addressing mental health on college campuses

Five )not ten) teaching practices inclusive of students with anxiety

Resilience through discomfort: Reframing anxiety and helpful strategies for the mathematics classroom

Learning and wellbeing: A collaborative approach to course design

Simulations offer transformational learning and anxiety reduction

NEFDC Board members

Population And Harvest Dynamics Of Midcontinent Sandhill Cranes, Aaron T. Pearse, Glen A. Sargeant, Gary L. Krapu, David A. Brandt

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Sandhill cranes (Antigone canadensis) inhabiting the midcontinent of North America have been hunted since the 1960s under management goals of maintaining abundance, retaining geographic distribution, and maximizing sustainable harvest. Some biologists have raised concerns regarding harvest sustainability because sandhill cranes have lower reproductive rates than other game birds. We summarized demographic information in an age‐structured matrix model to better understand population dynamics and harvest. Population indices and recovered harvest since the early 1980s suggest midcontinent sandhill cranes have experienced an average long‐term annual growth of 0.9%; meanwhile, harvest has increased 1.8% annually. Adult survival and recruitment rates estimated from field …

Host Transcriptional Response To Persistent Infection With A Live-Attenuated Porcine Reproductive And Respiratory Syndrome Virus Strain, Jayeshbhai Chaudhari, Chia-Sin Liew, Aspen M. Workman, Jean-Jack Riethoven, David J. Steffen, Sarah Vitosh-Sillman, Hiep Vu

Nebraska Center for Virology: Faculty Publications

Both virulent and live-attenuated porcine reproductive and respiratory syndrome virus (PRRSV) strains can establish persistent infection in lymphoid tissues of pigs. To investigate the mechanisms of PRRSV persistence, we performed a transcriptional analysis of inguinal lymphoid tissue collected from pigs experimentally infected with an attenuated PRRSV strain at 46 days post infection. A total of 6404 differentially expressed genes (DEGs) were detected of which 3960 DEGs were upregulated and 2444 DEGs were downregulated. Specifically, genes involved in innate immune responses and chemokines and receptors associated with T-cell homing to lymphoid tissues were down regulated. As a result, homing of virus-specific …

First Records Of Established Populations Of Ixodes Scapularis (Acari: Ixodidae) Collected From Three Nebraska Counties, Lindsey E. Nielsen, Roberto Cortinas, Paul D. Fey, Peter C. Iwen, David H. Nielsen

School of Veterinary and Biomedical Sciences: Faculty Publications

Reported cases of Lyme disease in Nebraska have been assumed to be imported from other endemic areas. Previous surveillance efforts provided no evidence of established populations as only individual specimens of Ixodes scapularis (Say) had been collected. In the winter of 2018, adult I. scapularis were found on a dog at Two Rivers State Recreation Area, Douglas County, prompting tick collection at the site and nearby natural areas. In May 2019, all life stages of host-seeking I. scapularis were collected using dragging and flagging techniques in sites located near the Platte River in Douglas, Sarpy, and Saunders counties. This is …