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Nebline, August 2020

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Be an Active Family with Outdoor Activities

EXTENSION OFFICE OPEN

Recipes of the month

What You Need to Know About Having a Well Drilled

Almost Time for Fall Lawn Seeding

Chiggers: Itching in all the Wrong Places

Collect – Share – Practice: Family Emergency Communication Plans

August Blooming Perennial Flowers

Garden Guide: Things to Do This Month

Creating Reading Routines During the Summer Months

HEART OF 4-H

4-H Youth and Volunteers Sew 1,789 Hats and Masks for Bryan Health

4-H’ers Problem Solve Real-Life Situations at County Life Challenge

Virtual State Public Speaking Contest Results

State Fair to Focus on Showcasing …

Detection Of Food Proteins In Human Serum Using Mass Spectrometry Methods, Abigail S. Burrows

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Allergenic peanut proteins are highly resistant to digestion and are detectable by immunoassays after gastrointestinal digestion. The application of liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods for in vivo detection of peptides originating from allergenic food proteins has not been thoroughly studied. The aim of this work was to develop an in vivo detection method for peanut proteins in serum using LC-MS/MS. The method(s) were validated by analyzing subject serum collected after peanut consumption.

Three de-complexation strategies were evaluated including (1) MS acquisition settings (i.e. inclusion, exclusion lists), (2) commercial depletion kits, and (3) organic solvent fractionation by discovery LC-MS/MS. Overall, …

Characterization Of Human Pyrroline-5-Carboxylate Reductase Enzymes Responsible For L-Proline Biosynthesis, Sagar Patel

Department of Biochemistry: Dissertations, Theses, and Student Research

Pyrroline-5-carboxylate reductases (EC 1.5.1.2) are important housekeeping enzymes of L-proline biosynthesis, which generate L-proline and influence redox cycling of NAD(P)H/NAD(P)⁺ to support cellular growth in all domains of life. Structural evidence from X-ray crystal structures of HsPYCR1 (PDB codes 5UAT, 5UAU, and 5UAV) shows both NADPH bound in the N-terminal Rao-Rossmann fold motif and an important hydrogen bond or proton donor role for Thr238 with L-P5C. The Thr238Ala mutation results in 10-fold loss in catalytic efficiency with varied L-P5C relative to the wild-type enzyme, thus indicating Thr238’s potential hydrogen bond and proton donation to L-P5C is critical for catalysis. …

Development Of Novel Catalysts For Hydrogen Gas Production From Biomass Compounds, Boanerges Bamaca

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Hydrothermal biomass gasification technologies (sub- and supercritical water gasification and aqueous-phase reforming) have considerable economic, environmental, and technical advantages over other energy-extensive technologies (e.g. natural gas reforming) for hydrogen gas production. However, lack of economically feasible and highly active catalysts is a main challenge that impedes upscaling of these technologies for hydrogen gas production.

The goal of this study was to develop innovative, economically feasible and active heterogeneous supported metal catalysts for hydrothermal processes for producing hydrogen gas from biomass-derived compounds. Because of its stable structure and chemical inertness, graphene was used as catalyst support. Graphene supported metal catalysts were …

Nitrate Dynamics And Source Within Nested Watersheds Of An Agricultural Stream, Nebraska, Usa, Galen Richards

School of Natural Resources: Dissertations, Theses, and Student Research

Nitrate concentrations have been increasing throughout the globe, primarily due to heightened agricultural activity. Nitrate concentrations in Bazile Creek, located in Northeastern Nebraska, have been steadily rising since the early 2000s. Groundwater nitrate concentrations within the Bazile Creek watershed are high (> 10 mg/L nitrate-N), and there is strong connectivity between groundwater and surface water systems. This study aimed to better understand temporal nitrate concentration variability within the watershed through sampling tributaries and the main channel during baseflow conditions. Nitrate source was also investigated though the use of dual δ¹⁵N and δ¹⁸O nitrate isotopes, with samples …

Feral Swine, Michael P. Glow, Nathan P. Snow, Kurt C. Vercauteren

Wildlife Damage Management Technical Series

Feral swine (Sus scrofa; Figure 1), also known as feral hogs, feral pigs, wild pigs, wild boar, or other similar derivations, are a non-native species considered to be one of the most destructive invasive terrestrial vertebrates in North America. While feral swine populations remained relatively small and confined in the continental United States following initial introductions by European explorers during the 15th century, substantial range expansion has occurred across every geographical region of the United States (Figure 2). This expansion has primarily been attributed to human-mediated movements, predominately for the purpose of establishing populations for recreational hunting, and facilitated by …

Application Of Software Engineering Principles To Synthetic Biology And Emerging Regulatory Concerns, Justin Firestone

School of Computing: Dissertations, Theses, and Student Research

As the science of synthetic biology matures, engineers have begun to deliver real-world applications which are the beginning of what could radically transform our lives. Recent progress indicates synthetic biology will produce transformative breakthroughs. Examples include: 1) synthesizing chemicals for medicines which are expensive and difficult to produce; 2) producing protein alternatives; 3) altering genomes to combat deadly diseases; 4) killing antibiotic-resistant pathogens; and 5) speeding up vaccine production.

Although synthetic biology promises great benefits, many stakeholders have expressed concerns over safety and security risks from creating biological behavior never seen before in nature. As with any emerging technology, there …

Life Cycle Assessment Of The Us Beef Processing Through Integrated Hybrid Approach, Shaobin Li, Yuwei Qin, Jeyamkondan Subbiah, Bruce Dvorak

Department of Civil and Environmental Engineering: Faculty Publications

Hybrid life cycle assessment (LCA) incorporating process-based and economic input-output (EIO)–based inventory data has been applied in various industries (e.g., wind energy, biofuel). Few hybrid LCA studies have been found in the food industry. This work analyzes the life cycle environmental impacts of the US beef processing industry using process-based and integrated hybrid LCA. The process-based inventory includes all resource inputs and waste outputs associated with a beef processing plant. The EIO-based inventory includes key activities missing in the process-based inventory, such as technical and management service, wood and paper, and industrial equipment. Ten environmental impact categories from TRACI v2.1 …

Taxonomy Of Echinostoma Revolutum And 37-Collar-Spined Echinostoma Spp.: A Historical Review, Jong-Yil Chai, Jaeeun Cho, Taehee Chang, Bong-Kwang Jung, Woon-Mok Sohn

Harold W. Manter Laboratory of Parasitology: Library Materials

Echinostoma flukes armed with 37 collar spines on their head collar are called as 37-collar-spined Echinostoma spp. (group) or ‘Echinostoma revolutum group’. At least 56 nominal species have been described in this group. However, many of them were morphologically close to and difficult to distinguish from the other, thus synonymized with the others. However, some of the synonymies were disagreed by other researchers, and taxonomic debates have been continued. Fortunately, recent development of molecular techniques, in particular, sequencing of the mitochondrial (nad1 and cox1) and nuclear genes (ITS region; ITS1-5.8S-ITS2), has enabled us to obtain highly …

An Evaluation Of Bird And Bat Mortality At Wind Turbines In The Northeastern United States, Daniel Y. Choi, Thomas W. Wittig, Bryan M. Kluever

United States Department of Agriculture Wildlife Services: Staff Publications

Wind energy offers substantial environmental benefits, but wind facilities can negatively impact wildlife, including birds and bats. Researchers and managers have made major efforts to chronicle bird and bat mortality associated with wind facilities, but few studies have examined the patterns and underlying mechanisms of spatial patterns of fatalities at wind facilities. Understanding the horizontal fall distance between a carcass and the nearest turbine pole is important in designing effective search protocols and estimating total mortality. We explored patterns in taxonomic composition and fall distance of bird and bat carcasses at wind facilities in the Northeastern United States using publicly …

Estimation Of Wildlife Damage From Federal Crop Insurance Data, Sophie Mckee, Stephanie A. Shwiff, Aaron M. Anderson

United States Department of Agriculture Wildlife Services: Staff Publications

BACKGROUND: Wildlife damage to crops is a persistent and costly problem for many farmers in the USA. Most existing estimates of crop damage have relied on direct assessment methods such as field studies conducted by trained biologists or surveys distributed to farmers. In this paper, we describe a new method of estimating wildlife damage that exploits federal crop insurance data. We focused our study on four crops: corn, soybean, wheat, and cotton, chosen because of their economic importance and their vulnerability to wildlife damage.

RESULTS: We determined crop-raiding hot spots across the USA over the 2015–2019 period and identified the …

Climate Change And Market-Based Insurance Feedbacks, Eric R. Holley, Adam Liska, Cory Walters, Geoffrey C. Friesen, Michael Hayes, Max J. Rudolph, Donald A. Wilhite

Adam Liska Papers

Climatic events have accounted for 91% of $1.05 trillion in insured costs for global catastrophic events from 1980 to 2016. Costs are driven by socio-economic development and increased frequency and severity of climatic disasters driven by climate change. Government policies to reduce systemic risk (e.g., cap-and-trade, carbon tax) have been a predominant approach for mitigation and adaptation. Alternatively, market-based incentives for climate change adaptation and mitigation already operate via the insurance industry to lessen impacts on society. Insurance feedbacks include changes in 1) premiums and insurance policies, 2) non-coverage, and 3) policy making and litigation. Alongside government policies, insurance feedbacks …

Metagenomic, Viral And Host Genetic Analyses Of Congenital Tremor In Pigs, Kylee Marie Sutton

School of Biological Sciences: Dissertations, Theses, and Student Research

The Pestivirus genus contains several viral species having a major impact on the livestock species. While there were only four major pestiviral species for a time, recent metagenomic sequencing approaches identified additional species, such as atypical porcine pestivirus (APPV). Congenital tremor was first identified almost a hundred years ago and since still has an impact on swine health. There are two main types of congenital tremor, type A and B, with type A congenital tremor further subcategorized based on causative agent. Until recently, type A-II congenital tremor did not have a known cause. Recent viral sequencing of affected samples, including …

Characterization Of Protein And Fat In Dairy Feeds And Implications On Digestibility And Milk Composition, Kassidy Kate Buse

Department of Animal Science: Dissertations, Theses, and Student Research

A variety of feedstuffs are used to comprise dairy rations, each with their own nutritional contributions. In order to best utilize these feedstuffs to maximize production, characterization of their chemical composition is needed. Through the use of both in vitro and in situ methods, not only can the composition of the feed be determined, but also its digestibility. Also, because of the important role they play in human health, omega 3 fatty acids have received increasing attention. Most individuals consuming Western diets do not meet the recommended requirement for omega 3 fatty acids, and one way to improve that is …

Advancing The Science Of Dry-Aged Beef, Felipe Azevedo Ribeiro

Department of Animal Science: Dissertations, Theses, and Student Research

In order to tightly control dry aging environmental conditions, we designed and built a computerized dry aging system that is capable of measuring and precisely controlling relative humidity (RH; ± 1%), temperature (± 0.5 °C), air flow (± 0.015m³/min), and mass loss (± 5 g). This dissertation addressed the effects of RH and ultimate pH on meat quality and sensory attributes of dry-aged beef. In study one, we learned that wet-aged steaks had higher L* (P = 0.01), a* (P = 0.03), and b* values (P < 0.001), lower discoloration scores (P < 0.05), and lower TBARS (thiobarbituric acid reactive substance) values (P = 0.03) than dry-aged treatments. Under prolonged …

Influence Of Strategic Supplementation And Genetic Potential For Milk Yield On Forage Digestibility, Amino Acid Utilization, And Livestock Production, Tasha M. King

Department of Animal Science: Dissertations, Theses, and Student Research

When consuming low-quality forages or at times when nutrient demands are high, ruminants may be unable to meet these requirements resulting in a state of negative energy balance. With feed costs contributing a large portion of production costs, providing dietary nutrients to maintain energy balance must be done so strategically. Additionally, producers must ensure consistent reproductive performance and offspring weaned to maintain a successful livestock enterprise. The objective of these studies was to evaluate the effect of glucogenic precursor supplementation and milk yield on forage digestibility, amino acid utilization, and livestock production. In experiment 1, a metabolism study was conducted …

A New Species Of Buprestis (S. Str.) Linnaeus, 1758 (Coleoptera: Buprestidae) From The Southwestern United States, Richard L. Westcott, Clint Burfitt

Insecta Mundi

A new species of Buprestis L., 1758, B. pinyoni Westcott and Burfitt (Coleoptera: Buprestidae), is described from Arizona, New Mexico, and Utah. It is compared to other species in the subgenus Buprestis.

Beetles (Coleoptera) In Cones Of Cycads (Cycadales) Of The Northern Hemisphere: Diversity And Evolution, William Tang, Guang Xu, Thomas Marler, Jibankumar Singh Khuraijam, Rita Singh, Anders J. Lindström, P. Radha, Stephen Rich, Khang Sinh Nguyen, Paul E. Skelley

Insecta Mundi

Three groups of beetles inhabit cones of cycads (Cycadales) in the northern hemisphere and are believed to be involved in their pollination. The primitive weevil subtribe Allocorynina (Coleoptera: Belidae) is restricted to the New World cycad genera Dioon Lindl. and Zamia L. One group of weevils (Curculionidae), found only in Cycas L., appears to be a relatively recent colonizer of northern hemisphere cycads. Members of the beetle subfamily Pharaxonothinae (Erotylidae) occur in all Asian and New World cycad genera. Phylogenetic trees of these beetles, based on DNA analysis and supported with morphological studies, are compared to patterns of continental drift …

Spatial Ecology And Resource Selection Of Bighorn Sheep (Ovis Canadensis) Ewes In A Prairie Badlands Population, Erin Wood

School of Natural Resources: Dissertations, Theses, and Student Research

The basic spatial ecology and habitat relationships of female bighorn sheep in Nebraska are poorly understood. Establishing seasonal patterns of space use and resource selection for this population at the margin of their historical and current range addresses a key knowledge gap and provides important baseline information for ongoing conservation efforts in Nebraska. We deployed GPS radio-collars on 56 adult ewes in western Nebraska to quantify seasonal space use, movements, and resource selection of ewes. To investigate spatial ecology, we quantified movements of ewes and the factors that influence home range size, seasonal use, and spatial stability across seasons. Home …

Pesticide Occurrence And Persistence Entering Recreational Lakes Residing In Watersheds Of Various Land Uses, Jessica Satiroff

Department of Environmental Engineering: Theses and Other Student Research

Common Use Pesticides (CUPs) are important for agricultural producers to sustain food production. As a result, regions with high rates of agricultural production, such as the Midwest, often have ubiquitous occurrences of pesticides in surface and groundwater ¹. Once pesticides are introduced into an ecosystem, pesticides have the potential for creating unwanted effects on non-target species and downstream environments ^2,3. However, pesticides do not come strictly from agricultural practices. Therefore, the research presented in this thesis focuses on the neonicotinoid and fungicide concentrations detected in recreational lakes as well as their persistence in these aquatic environments during the …

Eat My Quartz, Neil Celani-Morrell

School of Art, Art History, and Design: Theses and Student Creative Work

Pottery has a remarkable ability to tap into the human collective experience, so it is no surprise I express my own understanding of the world by working within the boundaries of “the pot”. With the capacity to communicate conceptual meaning and to serve as objects of utility, pottery not only dances in the notorious territory between art and craft, it embodies the human spirit.

My pots are totems of my existence and this work is an authentic representation of my creative journey. They are an homage to the key influences in my life which have informed and encouraged my creativity …

Formal Language Constraints In Deep Reinforcement Learning For Self-Driving Vehicles, Tyler Bienhoff

School of Computing: Dissertations, Theses, and Student Research

In recent years, self-driving vehicles have become a holy grail technology that, once fully developed, could radically change the daily behaviors of people and enhance safety. The complexities of controlling a car in a constantly changing environment are too immense to directly program how the vehicle should behave in each specific scenario. Thus, a common technique when developing autonomous vehicles is to use reinforcement learning, where vehicles can be trained in simulated and real-world environments to make proper decisions in a wide variety of scenarios. Reinforcement learning models, however, have uncertainties in how the vehicle acts, especially in a previously …

On-Farm Research And Student Engagement To Assess And Promote The Use Of Organic Amendments To Improve Agricultural Soil Health And Resilience Of Crop Production Systems In Nebraska, Agustin Jose Olivo

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

In nearly every production environment, there are opportunities to capture profits if waste streams can be further processed or enhanced to create “value added” products. On-farm research studies were initiated in 2019 at four locations across Nebraska to assess the impacts of livestock manure, cedar mulch from forestry management and coal char from sugar beet processing, on agricultural cropland. Study treatments included beef cattle manure (CM), beef cattle slurry (CS), coal char (CC), woody biomass (WB) CM+WB, CS+WB, CM+CC and control (CON; no organic amendment). Soil properties and corn yield were evaluated after a single growing season. Results indicate that …

Snowy Plover Activity In The Central Platte River Valley In May 2019, Bethany L. Ostrom, Andrew J. Caven, Jenna M. Malzahn, Alyx Vogel

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

During five of eight site visits between 14 and 27 May 2019 we detected up to four Snowy Plovers (Charadrius nivosus nivosus) in the Central Platte River Valley (CPRV) near Mormon Island, Hall County, Nebraska, and recorded their behavior using an instantaneous scan sampling approach. We recorded loafing (47%), foraging (43%), mating (4%), flying (4%), and external threat (1%) related behavior. Most notably, we documented a copulation event on 23 May. During the 10-day span from 14 to 23 May when Snowy Plovers were detected, river stage and discharge were near median levels, but from 23 to 27 …

His And Hers: Gender-Specific Design In Mid-Twentieth Century North America Through Film And Television, Morgan O'Shaughnessy

Architecture Masters of Science Program: Theses

The built environment exists as a variety of ‘spaces’ which are constructed by, and for, the people who occupy them. What is the relationship between social constructs of gender and our built environment in the 20^th century? How does film and television representing the mid-20^th century exemplify this relationship? The overall theme of this topic will include a positive relationship between social gender constructs and our built environment. Through the exploration of select film and television, this study attempts to answer the question of how gender-focused design reinforces traditional gender roles in North American society in mid-20^{th …}

Spatial And Temporal Patterns Of Bat Activity In A Southeast Nebraska Agricultural Landscape, Christopher Fill

School of Natural Resources: Dissertations, Theses, and Student Research

Intensive agriculture is associated with biodiversity loss and species decline. Yet wild species, such as bats, may provide critical ecosystem services to agriculture, even in transformed landscapes. In the United States, bats have been estimated to save the agricultural industry billions of dollars per year. However, white-nose syndrome and habitat loss have led to the decline of many bat species in North America, including the federally threatened northern long-eared bat, Myotis septentrionalis.

To better evaluate the effectiveness of these pest-controlling services, and to increase understanding of bat foraging behavior in these extreme landscapes, I deployed 11 grids of 24 detectors, …

Estimating A Fair Value For Standing Forage, Jay Parsons, Daren Redfearn, Mary E. Drewnoski

Cornhusker Economics

Several things need to be considered when deciding what value to place on standing forage. Forage prices reflect current inventories, demand, expected current season production and associated yield risk, and quality characteristics. Standing forage can be harvested in a number of different ways which need to be considered when pricing it. Below, we provide a few general thoughts and questions concerning harvesting method and comparable feed value to consider when pricing standing forage. We follow with a few examples illustrating pricing calculations for forage mechanically harvested as hay, haylage, and silage. We close with pricing considerations for forage harvested by …

Statistical Methodology To Establish A Benchmark For Evaluating Antimicrobial Resistance Genes Through Real Time Pcr Assay, Enakshy Dutta

Department of Statistics: Dissertations, Theses, and Student Research

Novel diagnostic tests are usually compared with gold standard tests for evaluating diagnostic accuracy. For assessing antimicrobial resistance (AMR) to bovine respiratory disease (BRD) pathogens, phenotypic broth microdilution method is used as gold standard (GS). The objective of the thesis is to evaluate the optimal cycle threshold (Ct) generated by real-time polymerase chain reaction (rtPCR) to genes that confer resistance that will translate to the phenotypic classification of AMR. Data from two different methodologies are assessed to identify Ct that will discriminate between resistance (R) and susceptibility (S). First, the receiver operating characteristic (ROC) curve was used to determine the …

Feeder Cattle Future Price Spreads: Opportunities To Hedge?, Elliott James Dennis

Extension Farm and Ranch Management News

First paragraph:

Feeder cattle future price spreads across all months have recovered to near pre-COVID-19 levels as quarantine restrictions and packing plant capacity issues have been mostly sorted out. For example, for the week of July 17, 2010 [sic], prices reached levels not seen since the beginning of March 2020. Pasture and corn progress are two factors that have the potential to push prices lower in the next coming months. Given current market conditions, producers have some options to lock in a margin.

Beneficial Effect Of Injected Air Into Subsurface Drip Irrigation (Sdi) On Plant Growth Using Runoff From A Feedlot, D. Dissanayake

School of Natural Resources: Dissertations, Theses, and Student Research

Due to water scarcity and increasing food demand, nonconventional water sources (e.g., human and animal wastewater) represent a valuable alternative to traditional water resources for agricultural use. Among these alternatives, treated animal wastewater, particularly feedlot runoff may represent a valuable solution in states like Nebraska due to its abundance. Subsurface drip irrigation (SDI) is a low-pressure micro-irrigation system that delivers water to the crop root zone through buried drip tapes with embedded emitters at fixed intervals. Despite multiple advantages (great water application uniformity, high water use efficiency, and improve fertilizer application), SDI can lead to poor aeration in the rhizosphere …