Digital Commons Network^™

Evaluating The Untapped Potential Of U.S. Conservation Investments To Improve Soil And Environmental Health, Andrea Basche, Katherine Tully, Nora L. Alvarez-Berrios, Julian Reyes, Laura Lengnick, Tabitha Brown, Jennifer M. Moore, Rachel E. Schattman, Lana Koepke Johnson, Gabrielle Roesch-Mcnally

Department of Agronomy and Horticulture: Faculty Publications

There is increasing enthusiasm around the concept of soil health, and as a result, new public and private initiatives are being developed to increase soil health-related practices on working lands in the United States. In addition, billions of U.S. public dollars are dedicated annually toward soil conservation programs, and yet, it is not well quantified how investment in conservation programs improve soil health and, more broadly, environmental health. The Environmental Quality Incentives Program (EQIP) is one of the major U.S. public conservation programs administered on privately managed lands for which public data are available. In this research, we developed a …

Validating A Common Tick Survey Method: Cloth‑Dragging And Line Transects, Pia L. Kjellander, Malin Aronsson, Ulrika A. Bergvall, Josep L. Carrasco, Madeleine Christensson, Per-Eric Lindgren, Mikael Åkesson, Petter Kjellander

Harold W. Manter Laboratory of Parasitology: Library Materials

Cloth-dragging is the most widely-used method for collecting and counting ticks, but there are few studies of its reliability. By using cloth-dragging, we applied a replicated line transects survey method, in two areas in Sweden with different Ixodes ricinus tick-densities (low at Grimsö and high at Bogesund) to evaluate developmental stage specific repeatability, agreement and precision in estimates of tick abundance. ‘Repeatability’ was expressed as the Intraclass Correlation Coefficient (ICC), ‘agreement’ with the Total Deviation Index (TDI) and ‘precision’ by the coefficient of variation (CV) for a given dragging distance. Repeatability (ICC) and agreement (TDI) were higher for the most …

Linking Mosquito Surveillance To Dengue Fever Through Bayesian Mechanistic Modeling, Clinton B. Leach, Jennifer A. Hoeting, Kim M. Pepin, Alvaro E. Eiras, Mevin B. Hooten, Colleen T. Webb

United States Department of Agriculture Wildlife Services: Staff Publications

Our ability to effectively prevent the transmission of the dengue virus through targeted control of its vector, Aedes aegypti, depends critically on our understanding of the link between mosquito abundance and human disease risk. Mosquito and clinical surveillance data are widely collected, but linking them requires a modeling framework that accounts for the complex non-linear mechanisms involved in transmission. Most critical are the bottleneck in transmission imposed by mosquito lifespan relative to the virus’ extrinsic incubation period, and the dynamics of human immunity. We developed a differential equation model of dengue transmission and embedded it in a Bayesian hierarchical …

Economic Analyses Of Federal Scientific Collections: Methods For Documenting Costs And Benefits, David E. Schindel, Economic Study Group Of The Interagency Working Group On Scientific Collections

Copyright, Fair Use, Scholarly Communication, etc.

Federal object-based scientific collections have been created to serve agency missions and, in a few cases, to comply with legislative and regulatory mandates. “Project collections” (those managed by the researchers who obtained them for restricted use) and their costs and benefits were considered too varied for standard methodologies that assess costs and benefits. In a few cases, departments and agencies are required by legislation or regulations to retain objects in long-term “institutional collections.” In most cases, decisions to retain objects are based on long-term costs relative to the perceived potential for benefits to taxpayers. Federal collections vary in their philosophies …

Flowers Are Essential To Maintain High Beetle Diversity (Coleoptera) In A Neotropical Rainforest Canopy, Susan Kirmse, Caroline S. Chaboo

University of Nebraska State Museum: Staff and Affiliates, Publications

Many hypotheses have been proposed to explain the megadiversity of angiosperms and insects. Flowers and their pollinators represent the most common terrestrial mutualistic interaction today and this is thought to have driven the evolution of angiosperms and their visitors. Within the framework of that interaction, this paper develops and tests two new hypotheses: 1) megadiversity of canopy beetles in tropical rainforests is largely based on flower resources, and 2) the majority of adult canopy beetles are adapted to visit flowers. To test hypothesis 1, the beetle fauna associated with 23 canopy tree species (13 families, 45 individuals) in a 1.4 …

Comparative Analyses Of Variable And Fixed Rate Irrigation And Nitrogen Management For Maize In Different Soil Types: Part I. Impact On Soil-Water Dynamics And Crop Evapotranspiration, Vasudha Sharma, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Understanding the soil-water dynamics and maize evapotranspiration (ETc) under variable rate irrigation (VRI) and variable rate fertigation (VRF) management with respect to soil spatial variability constitutes the basis for developing effective variable rate water and nitrogen management strategies. This long-term research was designed to quantify and compare the soil-water dynamics, including available water (AW), and ETc during vegetative and reproductive growth periods of VRI, fixed rate irrigation (FRI) and no-irrigation (NI) under fixed rate fertigation (FRF), VRF and pre-plant (PP) nitrogen management in three different soil types [Crete silt loam (S1); Hastings silty clay loam (S2) and Hastings silt loam …

The Birds Of The Nebraska Sandhills, Paul A. Johnsgard, Josef Kren

Zea E-Books Collection

This book provides basic information on all the species of birds that have been reliably reported from the Nebraska Sandhills region as of 2020. They include 46 permanent residents, 125 summer breeders, 125 migrants, and 102 rare or accidental species, totaling 398 species. Information on status, migration, and habitats is provided for all but the very rare and accidental species. There are also descriptions of 46 refuges, preserves, and other public-access natural areas in the region and seven suggested birding routes. The text contains more than 90,000 words and over 250 literature references along with more than 20 drawings, 9 …

Leaving The Twilight Zone: A Congressional Check On Treaty Termination, Cormac C. Broeg

Nebraska Law Bulletin (Selected Issues)

This essay argues that Congress can—by a statute expressing the will of both houses—protect a treaty from presidential termination. Under this framework, when Congress expressly states its opposition to the termination of a particular treaty, it moves the president’s order to terminate the treaty into this third category where the president can rely only upon enumerated powers. In Goldwater v. Carter, the Supreme Court found a challenge to presidential treaty termination non-justiciable without forming a majority opinion, However, a statutory protection of a treaty creates a different dispute which fails to implicate any of the political question factors articulated by …

Abundance And Diversity Of Dung Beetles (Coleoptera: Scarabaeoidea) As Affected By Grazing Management In The Nebraska Sandhills Ecosystem, Patrick M. Wagner, Gandura Omar Abagandura, Martha Mamo, Thomas Weissling, Ana Wingeyer, Jeffrey D. Bradshaw

Department of Entomology: Faculty Publications

Dung beetles (Coleoptera: Scarabaeoidea) serve a significant role in regulating ecosystem services on rangelands. However, the influence of grazing management on dung beetle communities remains largely unknown. The purpose of this study was to investigate dung beetle abundance and diversity throughout the grazing season in the Nebraska Sandhills Ecoregion. Grazing treatments included: continuous grazing (CONT), low-stocking rotational grazing (LSR), high-stocking rotational grazing (HSR), and no grazing (NG). The abundance and diversity of dung beetles were measured in the 2014 and 2015 grazing seasons using dung-baited pitfall traps. Dung beetle abundance for each grazing treatment was characterized through four indices: peak …

Effective Hair Styling Compositions And Processes, Yiqi Yang, Helan Xu, Kaili Song

Department of Textiles, Merchandising and Fashion Design: Faculty Publications

This disclosure relates to hair styling compositions and processes , and more particularly to compositions for disentangling or crosslinking hair that are useful in hair styling processes.

A Transgenic Pig Model Expressing A Cmv-Zsgreen1 Reporter Across An Extensive Array Of Tissues, Amy T. Desaulniers, Rebecca A. Cederberg, Elizabeth P. Carreiro, Channabasavaiah B. Gurumurthy, Brett R. White

Department of Animal Science: Faculty Publications

Since genetic engineering of pigs can benefit both biomedicine and agriculture, selecting a suitable gene promoter is critically important. The cytomegalovirus (CMV) promoter, which can robustly drive ubiquitous transgene expression, is commonly used at present, yet recent reports suggest tissue-specific activity in the pig. The objective of this study was to quantify ZsGreen1 protein (in lieu of CMV promoter activity) in tissues from pigs harboring a CMV-ZsGreen1 transgene with a single integration site. Tissue samples (n=35) were collected from neonatal hemizygous (n=3) and homozygous (n=3) piglets and ZsGreen1 abundance was determined via immunoblotting. ZsGreen1 was detected in all tissues, except …

Formal Concept Analysis Applications In Bioinformatics, Sarah Roscoe

School of Computing: Dissertations, Theses, and Student Research

Bioinformatics is an important field that seeks to solve biological problems with the help of computation. One specific field in bioinformatics is that of genomics, the study of genes and their functions. Genomics can provide valuable analysis as to the interaction between how genes interact with their environment. One such way to measure the interaction is through gene expression data, which determines whether (and how much) a certain gene activates in a situation. Analyzing this data can be critical for predicting diseases or other biological reactions. One method used for analysis is Formal Concept Analysis (FCA), a computing technique based …

Genomic Evidence Suggests Further Changes Of Butterfly Names, Jing Zhang, Qian Cong, Jinhui Shen, Pal A. Opler, Nick V. Grishin

The Taxonomic Report of the International Lepidoptera Survey

Further genomic sequencing of butterflies by our research group expanding the coverage of species and specimens from different localities, coupled with genome-scale phylogenetic analysis and complemented by phenotypic considerations, suggests a number of changes to the names of butterflies, mostly those recorded from the United States and Canada. Here, we present evidence to support these changes. The changes are intended to make butterfly classification more internally consistent at the genus, subgenus and species levels. That is, considering all available evidence, we attempt to assign similar taxonomic ranks to the clades of comparable genetic differentiation, which on average is correlated with …

Distribution Of Antibiotic-Resistant Enterobacteriaceae Pathogens In Potable Spring Water Of Eastern Indian Himalayas: Emphasis On Virulence Gene And Antibiotic Resistance Genes In Escherichia Coli, Ashish Kumar Singh, Saurav Das, Santosh Kumar, Varsha Rani Gajamer, Ishfaq Nabi Najar, Yanchen D. Lepcha, Hare Krishna Tiwari, Samer Singh

Department of Agronomy and Horticulture: Faculty Publications

Every year millions of people die due to fatal waterborne diseases around the world especially in developing countries like India. Sikkim, a northeastern state of India, greatly depends on natural water sources. About 80% of the population of Sikkim depends on natural spring water for domestic as well as agricultural use. Recent waterborne disease outbreaks in the state raises a concerning question on water quality. In this study, we analyzed water quality especially for the detection of Enterobacteriaceae members from four districts of the state. Isolation with selective culture media techniques and taxonomic characterization of Enterobacteriaceae bacteria with 16S rRNA …

Trophic Cascades Alter Eco-Evolutionary Dynamics And Body Size Evolution, Thomas M. Luhring, John Delong

School of Biological Sciences: Faculty Publications

Trait evolution in predator–prey systems can feed back to the dynamics of interacting species as well as cascade to impact the dynamics of indirectly linked species (eco-evolutionary trophic cascades; EETCs). A key mediator of trophic cascades is body mass, as it both strongly influences and evolves in response to predator–prey interactions. Here, we use Gillespie ecoevolutionary models to explore EETCs resulting from top predator loss and mediated by body mass evolution. Our four-trophic-level food chain model uses allometric scaling to link body mass to different functions (ecological pleiotropy) and is realistically parameterized from the FORAGE database to mimic the parameter …

Assessing The Environmental Impacts Of Effluent From Aerobic Treatment Units, Sarah Deeb, Tamylles Souza Da Costa, Zahaan Eswani, Christopher Struchtemeyer

RURALS: Review of Undergraduate Research in Agricultural and Life Sciences

Aerobic treatment units (ATUs) serve as an alternative to traditional septic systems in many rural homes that lack access to municipal utilities. ATU effluent often flows directly into ditches that contain overgrown vegetation and rely on sunlight for disinfection. To date, it is unclear if effluent gets properly disinfected in these ditches, which is problematic since they often flow directly into major recreational water bodies. The goal of this work was to determine if ATUs negatively impact the environment by quantifying Escherichia coli and thermotolerant coliforms in: 1) ATU effluent, 2) Disposal ditches that received ATU effluent, and 3) Water …

Using Triallelic Snps For Determining Parentage In North American Yak (Bos Grunniens) And Estimating Cattle (B. Taurus) Introgression, Theodore S. Kalbfleisch, Jessica L. Petersen, R. G. Tait Jr., Jiansheng Qiu, Veronica Basnayake, Peter H. Hackett, Michael P. Heaton

Department of Animal Science: Faculty Publications

Background: Genetic testing for pedigree accuracy is critical for managing genetic diversity in North American (NA) yak (Bos grunniens ), a population expanded mostly from imported zoological park specimens. DNA testing also enhances species conservation by identifying recent B. taurus F1 hybrid ancestors (within three generations). Biallelic single nucleotide polymorphisms (SNPs) can accomplish either task, but increases the marker count and costs necessary to achieve both. Our aim was to identify novel, multifunctional, triallelic yak SNPs (tySNPs), with each having two alleles for yak parentage testing, and a third allele for identifying recent cattle introgression.

Methods: Genome sequences were …

Seasonal Grassland Productivity Forecast For The U.S. Great Plains Using Grass-Cast, Melannie D. Hartman, William J. Parton, Justin D. Derner, Darin K. Schulte, William K. Smith, Dannele E. Peck, Ken A. Day, Stephen J. Del Grosso, Susan Lutz, Brian Fuchs, Maosi Chen, Wei Gao

National Drought Mitigation Center: Faculty Publications

Every spring, ranchers in the drought-prone U.S. Great Plains face the same difficult challenge —trying to estimate how much forage will be available for livestock to graze during the upcoming summer grazing season. To reduce this uncertainty in predicting forage availability, we developed an innovative new grassland productivity forecast system, named Grass-Cast, to provide science-informed estimates of growing season aboveground net primary production (ANPP). Grass-Cast uses over 30 yr of historical data including weather and the satellite-derived normalized vegetation difference index (NDVI)—combined with ecosystem modeling and seasonal precipitation forecasts—to predict if rangelands in individual counties are likely to produce below-normal, …

“This Is How We Do It”: A Descriptive Analysis Of The Musical Elements And The Black Church Cultural Influences In Adolphus Hailstork’S I Will Lift Up Mine Eyes: A Cantata For Tenor, Choir, And Chamber Orchestra, Alfonzo Cooper Jr

Glenn Korff School of Music: Dissertations, Theses, Student Creative Work, and Performances

This document analyzes Adolphus Hailstork’s cantata, I Will Lift Up Mine Eyes, featuring text from Psalms 121, 13, and 23. The author contends that Hailstork uses cultural musical elements of the Black Church throughout the entire composition. Beginning with a brief biography of the composer, the author then provides a movement-by-movement examination of salient musical features and Black Church cultural traditions Hailstork employs to highlight the text of his selected psalms.

Advisor: William Shomos

Transcendentalist Sympathies: A Contextual Study Of The Wound-Dresser, Jared Hiscock

Glenn Korff School of Music: Dissertations, Theses, Student Creative Work, and Performances

My document takes as its subject The Wound-Dresser by American composer John Coolidge Adams (b. 1947). Published in 1988, this twenty minute work for baritone voice and orchestra remains Adams’s sole contribution to the non-operatic solo voice repertoire.

In The Wound-Dresser Adams grapples with the historical churning of his own times by looking to Ralph Waldo Emerson, Walt Whitman, and Charles Ives. A brief biography of Ralph Waldo Emerson begins the document in order to elucidate Emerson’s connection to Adams and Whitman. I then introduce Walt Whitman, the author of the text used in The Wound-Dresser and suggest that Whitman’s …

The Granulate Cutworm (Lepidoptera: Noctuidae): Biological Parameters Under Controlled Conditions, Host Plants, And Distribution In The Americas, Alexandre Specht, Fernando M. S. Dias, Germán San Blas, Vânia F. Roque-Specht, Mirna M. Casagrande, Olaf H. H. Mielke, Débora Goulart Montezano, Izailda Barbosa Santos, Silvana Vieira De Paula Moraes, Thomas E. Hunt, Juaci V. Malaquias, Felipe A. D. Bonfin, Paulo V. M. Vieira

Department of Entomology: Faculty Publications

Feltia subterranea (Fabricius), commonly known as the granulate cutworm, is a common species of owlet moths (Noctuidae) of major agricultural importance, widely distributed in Nearctic and Neotropical regions. This study was conducted to determine the species biological parameters, gather information about its larval host plants, and assess the agricultural significance of this species in the Americas. The viability of the egg, larval, pupal stages, and prepupal period was 98, 98, and 100%, respectively, under laboratory conditions. The average duration of the egg, larval, pupal stages, and prepupal period was 3, 17, 4, and 13 d, respectively. All laboratory-reared larvae developed …

Assessing The Quantification Of Soy Protein In Incurred Matrices Using Targeted Lc-Ms/Ms, Jenna Krager

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Soy-derived ingredients are commonly added as auxiliary components to a diverse range of food products. The versatile end-applications of commercially processed soy ingredients is concerning for the safety of allergic consumers. Immunological detection of soy proteins in food matrices has some drawbacks, including loss of epitope binding and matrix masking. Therefore, the aim of this study was to assess naturally incurred matrices with an existing liquid-chromatography tandem mass spectrometry (LC-MS/MS) workflow to determine the recovery of total soy protein.

The existing LC-MS/MS workflow was time intensive. The peptide responses of five soy protein targets were compared between two dilution methods …

Career And Technical Education Teachers’ Beliefs About Developing Students’ Motivation To Write, Katie Graham

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Students in the U.S. write poorly. Although postsecondary and workplace writing expectations continue to rise, writing instruction has largely remained unchanged over time. As a result, student writing outcomes continue to fall below expectations from both an assessment and hirable proficiency standpoint. Career and Technical Education (CTE) provides a unique opportunity to motivate students who are otherwise disengaged in writing (ACTE, 2009). The benefits of helping students develop their writing-self efficacy has well-documented support (Bruning & Horn, 2009; Bandura, 1997; Pajares 2003; Pajares & Valiante, 2006). However, little is known about the writing opportunities provided in CTE classes or CTE …

Dissecting The Regulatory Network Of Sphingolipid Biosynthesis In Plants, Ariadna Gonzalez-Solis

Department of Biochemistry: Dissertations, Theses, and Student Research

Sphingolipids are a diverse group of lipids recognized as important components of cellular membranes and regulators of processes during development and in response to environmental stresses. Serine palmitoyltransferase (SPT) catalyzes the first step in sphingolipid biosynthesis and is a primary regulatory point for homeostasis. ORM proteins have been identified as negative regulators of SPT activity, however the mechanistic details of the regulation are only beginning to be understood. In this work, we show that ORM1 and ORM2 are essential for life cycle completion in Arabidopsis thaliana. Furthermore, the study of a structural ORM1 variant provided information about a transmembrane …

Investigating Microbial And Host Factors That Modulate Severity Of Clostridioides Difficile Associated Disease, Armando Lerma

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Clostridioides difficile is recognized as one of the most important pathogens in hospital and community healthcare settings. The clinical outcome of infection of toxigenic C. difficile infection (CDI) ranges from asymptomatic colonization to fulminant pseudomembranous colitis and death. In recent studies, it has been suggested that a high proportion of nosocomial CDI cases are transmitted from asymptomatic carriers which might be acting as infection reservoirs. Understanding what causes the different responses to infection could lead to the development of novel prevention and treatment strategies. Although several explanations have been proposed to explain variations in susceptibility, understanding of the exact mechanisms …

Coyotes, Rick Tischaefer

Wildlife Damage Management Technical Series

The coyote (Canis latrans; Figure 1) is a medium-sized member of the canid family. Once primarily found in western deserts and grasslands, coyotes have expanded their range across North America and into diverse habitats, including urban areas. This expansion occurred during a time of extensive habitat change and efforts by people to suppress coyote populations to prevent damage. Coyotes can cause a variety of conflicts related to agriculture, natural resources, property, and human health and safety. This document highlights a variety of methods for reducing those conflicts. Coyotes are a highly adaptable species and may become habituated to some management …

Spotted Owls And Forest Fire: Comment, Gavin M. Jones, R. J. Gutiérrez, William M. Block, Peter C. Carlson, Emily J. Comfort, Samuel A. Cushman, Raymond J. Davis, Stephanie A. Eyes, Alan B. Franklin, Joseph L. Ganey, Shaula Hedwall, John J. Keane, Rodd Kelsey, Damon B, Lesmeister, Malcolm P. North, Susan L. Roberts, Jeremy T. Rockweit, Jamie S. Sanderlin, Sarah C. Sawyer, Ben Solvesky, Douglas J. Tempel, Ho Yi Wan, A. Leroy Westerling, Gary C. White, M. Zachariah Peery

United States Department of Agriculture Wildlife Services: Staff Publications

Western North American forest ecosystems are experiencing rapid changes in disturbance regimes because of climate change and land use legacies (Littell et al. 2018). In many of these forests, the accumulation of surface and ladder fuels from a century of fire suppression, coupled with a warming and drying climate, has led to increases in the number of large fires (Westerling 2016) and the proportion of areas burning at higher severity (Safford and Stevens 2017, Singleton et al. 2018). While the annual area burned by fire is still below historical levels (Taylor et al. 2016), some forest types in the west …

Trypa-No! Contributes To The Elimination Of Gambiense Human African Trypanosomiasis By Combining Tsetse Control With “Screen, Diagnose And Treat” Using Innovative Tools And Strategies, Joseph Mathu Ndung’U, Alain Boulangé, Albert Picado, Albert Mugenyi, Allan Mortensen, Andrew Hope, Brahim Guihini Mollo, Bruno Bucheton, Charles Wamboga, Charles Waiswa, Dramane Kaba, Enock Matovu, Fabrice Courtin, Gala Garrod, Geoffrey Gimonneau, Georgina Bingham, Hassane Mahamat Hassane, Inaki Tirados, Isabel Saldanha, Jacques Kabore, Jean Baptiste Rayaisse, Jean Mathieu Bart, Jessica Lingley, Johan Esterhuizen, Joshua Longbottom, Justin Pulford, Lingue Kouakou, Lassina Sanogo, Lucas Cunningham, Mamadou Camara, Mathurin Koffi, Michelle Stanton, Mike Lehane, Moise Saa Kagbadouno, Oumou Camara, Paul Bessell, Peka Mallaye, Philippe Solano, Richard Selby, Sophie Dunkley

Department of Entomology: Faculty Publications

Gambiense human African trypanosomiasis (g-HAT) is the chronic form of sleeping sickness caused by Trypanosoma brucei gambiense in West and Central Africa, while Trypanosoma brucei rhodesiense causes an acute form in eastern Africa. g-HAT is targeted for elimination as a public health problem by 2020 and 0 transmission by 2030 [1,2]. Control of g-HAT is largely based on identification and treatment of infected individuals, supplemented by control of the tsetse fly vectors [3]. There has been growing evidence that when both tsetse control and case identification activities are carried out simultaneously in the same geographies, elimination of the disease is …

Exploring A Novel Substrate-Borne Vibratory Signal In The Wolf Spider Schizocosa Floridana, Malcolm F. Rosenthal, Eileen Hebets, Rowan H. Mcginley, Cody Raiza, James Starrett, Lin Yan, Damian O. Elias

Eileen Hebets Publications

Animals communicate using a diversity of signals produced by a wide array of physical structures. Determining how a signal is produced provides key insights into signal evolution. Here, we examine a complex vibratory mating display produced by male Schizocosa floridana wolf spiders. This display contains three discrete substrate-borne acoustic components (known as “thumps”, “taps”, and “chirps”), each of which is anecdotally associated with the movement of a different body part (the pedipalps, legs, and abdomen respectively). In order to determine the method of production, we employ a combination of high-speed video/audio recordings and SEM imaging of possible sound-producing structures. Previous …

Testing The Hypothesized Antipredator Defence Function Of Stridulation In The Spiny Orb-Weaving Spider, Micrathena Gracilis, Tyler B. Corey, Eileen A. Hebets

Eileen Hebets Publications

The observable diversity of antipredator defenses across organisms demonstrates predation’s impact on trait evolution. The functions of many traits that are presumed to have an antipredator function have never been directly tested. The spiny orb-weaving spider, Micrathena gracilis, for example, stridulates when grasped. While stridulation was first hypothesized to be an antipredator defense nearly 50 years ago, no data exist to support this hypothesis. To explore the form and function of M. gracilis stridulation, we first quantified the behavioral and acoustical properties of sound production. Next, using laboratory assays, we directly tested the effect of stridulation on survival with …