Digital Commons Network^™

Elucidation Of The Microrna Transcriptome In Western Corn Rootworm Reveals Its Dynamic And Evolutionary Complexity, Xiaozeng Yang, Elane Fishilevich, Marcelo A. German, Premchand Gandra, Robert E. Mcewan, André Billion, Eileen Knorr, Andreas Vilcinskas, Kenneth E. Narva

Department of Entomology: Faculty Publications

Diabrotica virgifera virgifera (western corn rootworm, WCR) is one of the most destructive agricultural insect pests in North America. It is highly adaptive to environmental stimuli and crop protection technologies. However, little is known about the underlying genetic basis of WCR behavior and adaptation. More specifically, the involvement of small RNAs (sRNAs), especially microRNAs (miRNAs), a class of endogenous small non-coding RNAs that regulate various biological processes, has not been examined, and the datasets of putative sRNA sequences have not previously been generated for WCR. To achieve a comprehensive collection of sRNA transcriptomes in WCR, we constructed, sequenced, and analyzed …

Multi-Level Movement Response Of Invasive Wild Pigs (Sus Scrofa) To Removal, Guillaume Bastille-Rousseau, Peter E. Schlichting, David A. Keiter, Joshua B. Smith, John C. Kilgo, George Wittemyer, Kurt C. Vercauteren, James C. Beasley, Kim M. Pepin

United States Department of Agriculture Wildlife Services: Staff Publications

BACKGROUND: Lethal removal of invasive species, such as wild pigs (Sus scrofa), is often the most efficient approach for reducing their negative impacts. Wild pigs are one of the most widespread and destructive invasive mammals in the USA. Lethal management techniques are a key approach for wild pigs and can alter wild pig spatial behavior, but it is unclear how wild pigs respond to the most common removal technique, trapping.We investigated the spatial behavior of wild pigs following intensive removal of conspecifics via trapping at three sites within the Savannah River Site, SC, USA. We evaluated changes in …

Evaluating The Effects Of Mountain Beaver (Aplodontia Rufa) Management On Conifer Stocking In Western Oregon, Jimmy D. Taylor, Vanessa M. Petro

United States Department of Agriculture Wildlife Services: Staff Publications

Mountain beaver (Aplodontia rufa) is the most primitive rodent species in North America and is endemic to the Pacific Northwest, USA. Within their range, mountain beaver cause more conflict with conifer forest regeneration than any other vertebrate species. Most damage occurs as a result of clipping and browsing new seedlings, which reduces stocking density and delays stand development. An integrated approach using trapping and a registered toxicant (baiting) has been suggested as the most efficacious means to reduce seedling loss during stand initiation. We evaluated this management strategy in intensively managed conifer stands across two mountain ranges in …

Principal Economic Effects Of Cormorant Predation On Catfish Farms, Carole R. Engle, Terrel W. Christie, Brian S. Dorr, Ganesh Kumar, Luke A. Roy, Anita M. Kelly

United States Department of Agriculture Wildlife Services: Staff Publications

Substantial economic losses of farmed catfish to fish-eating birds such as the double-crested cormorant, Phalacrocorax auritus, continue to be reported on U.S. catfish farms. An economic analysis was conducted of the on-farm effects of both the increased expenditures to scare fish-eating birds from catfish farms and of the value of the catfish that were consumed by cormorants. A survey was conducted of U.S. catfish farmers in the Delta region of Mississippi and Arkansas, to obtain farm-level data on expenditures to scare birds. Estimations of the lost revenue from catfish consumed by cormorants were developed from a concurrent study on …

Variation In Angiostrongylus Cantonensis Infection In Definitive And Intermediate Hosts In Hawaii, A Global Hotspot Of Rat Lungworm Disease, Chris Niebuhr, Shane R. Siers, Israel Leinbach, Lisa M. Kaluna, Susan I. Jarvi

United States Department of Agriculture Wildlife Services: Staff Publications

Angiostrongylus cantonensis (rat lungworm) is a tropical and subtropical parasitic nematode, with infections in humans causing angiostrongyliasis (rat lungworm disease), characterized by eosinophilic meningitis. Hawaii has been identified as a global hotspot of infection, with recent reports of high infection rates in humans, as well as rat definitive and snail intermediate hosts. This study investigated variation in A. cantonensis infection, both prevalence and intensity, in wild populations of two species of rats (Rattus exulans and R. rattus) and one species of snail (Parmarion martensi). An overall infection prevalence of 86.2% was observed in P. martensi and …

Peptide Elisa And Fret-Qpcr Identified A Significantly Higher Prevalence Of Chlamydia Suis In Domestic Pigs Than In Feral Swine From The State Of Alabama, Usa, Md Monirul Hoque, Folasade Adekanmbi, Subarna Barua, Kh. Shamsur Rahman, Virginia Aida, Brian Anderson, Anil Poudel, Anwar Kalalah, Sara Bolds, Steven Madere, Steven Kitchens, Stuart Price, Vienna Brown, B. Graeme Lockaby, Constantinos S. Kyriakis, Bernhard Kaltenboeck, Chengming Wang

United States Department of Agriculture Wildlife Services: Staff Publications

Chlamydia suis is an important, highly prevalent, and diverse obligate intracellular pathogen infecting pigs. In order to investigate the prevalence and diversity of C. suis in the U.S., 276 whole blood samples from feral swine were collected as well as 109 fecal swabs and 60 whole blood samples from domestic pigs. C. suis-specific peptide ELISA identified anti-C. suis antibodies in 13.0% of the blood of feral swine (26/276) and 80.0% of the domestic pigs (48/60). FRET-qPCR and DNA sequencing found C. suis DNA in 99.1% of the fecal swabs (108/109) and 21.7% of the whole blood (13/60) of …

Behavioral State Resource Selection In Invasive Wild Pigs In The Southeastern United States, Lindsay M. Clontz, Kim M. Pepin, Kurt C. Vercauteren, James C. Beasley

United States Department of Agriculture Wildlife Services: Staff Publications

Elucidating correlations between wild pig (Sus scrofa) behavior and landscape attributes can aid in the advancement of management strategies for controlling populations. Using GPS data from 49 wild pigs in the southeastern U.S., we used hidden Markov models to define movement path characteristics and assign behaviors (e.g., resting, foraging, travelling). We then explored the connection between these behaviors and resource selection for both sexes between two distinct seasons based on forage availability (i.e., low forage, high forage). Females demonstrated a crepuscular activity pattern in the high-forage season and a variable pattern in the low-forage season, while males exhibited nocturnal activity …

Efficacy And Risks From A Modified Sodium Nitrite Toxic Bait For Wild Pigs, Nathan P. Snow, Jason Wishart, Justin A. Foster, Linton D. Staples, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

BACKGROUND: Wild pigs (Sus scrofa) are a destructive invasive species throughout many regions of the world. In 2018, a field evaluation of an early prototype of a sodium nitrite (SN) toxic bait in the United States revealed wild pigs dropped large amounts of the toxic bait outside the pig-specific bait stations while feeding, and thus subsequent hazards for non-target animals. We modified the SN-toxic bait formulation, the design of the bait station, and the baiting strategy to reduce dropped bait. We tested the modifications in Queensland, Australia (December 2018), Alabama, USA (August 2019), and Texas, USA (March 2020) …

Daily And Landscape Influences Of Species Visitation To Toxic Bait Sites For Wild Pigs, Nathan P. Snow, Joseph M. Halseth, Michael P. Glow, Michael Lavelle, Justin Fischer, Eric H. Vannatta, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Toxic baiting of wild pigs (Sus scrofa) is a potential new tool for population control and damage reduction in the United States. Use of toxic bait sites by non‐target species is concerning because of the risks posed from exposure to a toxic bait. A 2018 field trial in northern Texas, USA, examining the efficacy of a prototype toxic bait (HOGGONE®, containing 10% sodium nitrite) revealed unexpected hazards to non‐target species, primarily passerine birds, from consuming toxic bait spilled outside of bait stations by wild pigs. The hazards jeopardize the ability to register HOGGONE as a tool for controlling …

Effects Of Social Structure And Management On Risk Of Disease Establishment In Wild Pigs, Anni Yang, Peter E. Schlichting, Bethany Wight, Wesley M. Anderson, Sarah M. Chinn, Mark Q. Wilber, Ryan S. Miller, James C. Beasley, Raoul Boughton, Kurt C. Vercauteren, George Wittemyer, Kim M. Pepin

United States Department of Agriculture Wildlife Services: Staff Publications

1. Contact heterogeneity among hosts determines invasion and spreading dynamics of infectious disease, thus its characterization is essential for identifying effective disease control strategies. Yet, little is known about the factors shaping contact networks in many wildlife species and how wildlife management actions might affect contact networks.

2. Wild pigs in North America are an invasive, socially structured species that pose a health concern for domestic swine given their ability to transmit numerous devastating diseases such as African swine fever (ASF). Using proximity loggers and GPS data from 48 wild pigs in Florida and South Carolina, USA, we employed a …

Distribution And Abundance Of Scaup Using Baitfish And Sportfish Farms In Eastern Arkansas, Stephen A. Clements, Brian S. Dorr, Brian Davis, Luke A. Roy, Carole R. Engle, Katie C. Hanson-Dorr, Anita M. Kelly

United States Department of Agriculture Wildlife Services: Staff Publications

Arkansas' bait- and sportfish facilities are commonly used by various piscivorous bird species, including lesser scaup (Aythya affinis) and greater scaup (A. marila) that consume substantial quantities of fish. To mediate this predation, farmers implement extensive bird harassment programs that create additional costs to fish loss, thus research investigating the distribution and abundance of scaup is needed to help farmers allocate their bird harassment efforts more efficiently. In winters 2016–2017 and 2017–2018 we conducted 1,368 pond surveys to investigate pond use by scaup on farms during birds' regular wintering period (i.e., November–March). We used intrinsic and …

Avian Influenza A Viruses Reassort And Diversify Differently In Mallards And Mammals, Ketaki Ganti, Anish Bagga, Juliana Dasilva, Samuel S. Shepard, John R. Barnes, Susan A. Shriner, Katia Koelle, Anice C. Lowen

United States Department of Agriculture Wildlife Services: Staff Publications

Reassortment among co-infecting influenza A viruses (IAVs) is an important source of viral diversity and can facilitate expansion into novel host species. Indeed, reassortment played a key role in the evolution of the last three pandemic IAVs. Observed patterns of reassortment within a coinfected host are likely to be shaped by several factors, including viral load, the extent of viral mixing within the host and the stringency of selection. These factors in turn are expected to vary among the diverse host species that IAV infects. To investigate host differences in IAV reassortment, here we examined reassortment of two distinct avian …

Recolonizing Carnivores: Is Cougar Predation Behaviorally Mediated By Bears?, Kristin N. Engebretsen, Jon P. Beckmann, Carl W. Lackey, Alyson M. Andreasen, Cody Schroeder, Pat Jackson, Julie K. Young

United States Department of Agriculture Wildlife Services: Staff Publications

Conservation and management efforts have resulted in population increases and range expansions for some apex predators, potentially changing trophic cascades and foraging behavior. Changes in sympatric carnivore and dominant scavenger populations provide opportunities to assess how carnivores affect one another. Cougars (Puma concolor) were the apex predator in the Great Basin of Nevada, USA, for over 80 years. Black bears (Ursus americanus) have recently recolonized the area and are known to heavily scavenge on cougar kills. To evaluate the impacts of sympatric, recolonizing bears on cougar foraging behavior in the Great Basin, we investigated kill sites of 31 cougars between …

The Consequences Of Predators Without Prey, Mitchell A. Parsons, Thomas M. Newsome, Julie K. Young

United States Department of Agriculture Wildlife Services: Staff Publications

Rapid and ongoing environmental change is leading to scenarios where marine and terrestrial predators are persisting without prey, either by scavenging or using anthropogenic foods. Despite investigations into the effects of predator presence or absence on prey behavior and ecology, little research has assessed the effect of prey absence on predators. Here, we synthesize research on scavenging and the use of anthropogenic resources by marine and terrestrial predators; hypothesize how the use of these resources may change predator behavior with respect to their social structure, space use, life history, and individual behavioral traits; and illustrate how these changes are likely …

Improving Evaluation Of Nonlethal Tools For Carnivore Management And Conservation: Evaluating Fladry To Protect An Endangered Species From A Generalist Mesocarnivore, R. M. Windell, L. L. Bailey, J. K. Young, T. M. Livieri, D. A. Eads, S. W. Breck

United States Department of Agriculture Wildlife Services: Staff Publications

Carnivore conservation and management are global research priorities focused on reversing population declines of imperiled species and identifying more effective and humane management of generalist carnivores with thriving populations. Nonlethal methods to mitigate conflict are increasingly used to advance conservation objectives; however, there is limited knowledge about the effectiveness of many nonlethal methods. We tested a nonlethal tool (fladry), that serves as a barrier to deter wolves Canis lupus and coyotes Canis latrans, for its efficacy at preventing coyotes from using prairie dog Cynomys ludovicianus colonies, the primary prey for critically endangered black-footed ferrets Mustela nigripes. We used …

Social Media As A Window Into Human-Wildlife Interactions And Zoonotic Disease Risk: An Examination Of Wild Pig Hunting Videos On Youtube, Hailey E. Mclean, Lauren M. Jaebker, Aaron M. Anderson, Tara L. Teel, Alan D. Bright, Stephanie A. Shwiff, Keith M. Carlisle

United States Department of Agriculture Wildlife Services: Staff Publications

Wild pigs (Sus scrofa) damage agriculture and the environment, as well as transmit diseases to animals and people. Hunters are particularly vulnerable to zoonotic disease risks when harvesting wild pigs. Management agencies have endeavored to inform the public about disease risks associated with wild pigs and best practices for mitigating such risks. However, the extent that this guidance has reached hunters and influenced their practices is unclear. We approached this topic through an analysis of wild pig hunting videos on YouTube. We found evidence of relatively few behaviors and communications regarding disease risks and best practices for personal …

Relative Acceptance Of Brodifacoum Pellets And Soft Bait Sachets By Polynesian Rats (Rattus Exulans) On Wake Atoll, Peter J. Kappes, Shane R. Siers

United States Department of Agriculture Wildlife Services: Staff Publications

Removing invasive rats from island ecosystems using rodenticides has proven conservation benefits and is an important management tool for conserving and restoring island ecosystems. However, rodenticide-based eradications can fail if not all rats consume enough bait to result in lethal toxicosis. A recent post-operational review of a failed attempt to eradicate rats from Wake Atoll suggested that some individuals may not have ingested a lethal dose of rodenticide due to potential dietary and/or sensory preferences developed via regular access to anthropogenic food sources. These food sources may be higher in fats and oils, possessing different sensory properties (e.g., softer, chewier, …

Welfare Performance Of Three Foothold Traps For Capturing North American River Otters Lontra Canadensis, Matthew J. Lovallo, H. B. White, J. D. Erb, M. S. Peek, Thomas J. Deliberto

United States Department of Agriculture Wildlife Services: Staff Publications

Foothold traps are effective tools for the live capture and restraint of wildlife for management and research. Successful river otter Lontra canadensis restoration programs throughout North America used them extensively. Restoration programs used a variety of methods and models of foothold traps, but comprehensive efforts to describe and quantify injuries associated with river otter captures have been limited. We evaluated injuries of river otters caught in three commercially available models of foothold traps including the number 11 double long-spring with standard jaws, the number 11 double long-spring with double jaws, and the number 2 coil-spring trap. Based on examinations of …

Scaup Depredation On Arkansas Baitfish And Sportfish Aquaculture, Stephen A. Clements, Brian S. Dorr, J. Brian Davis, Luke A. Roy, Carole R. Engle, Katie C. Hanson-Dorr, Anita M. Kelly

United States Department of Agriculture Wildlife Services: Staff Publications

Lesser scaup (Aythya affinis) and greater scaup (A. marila), hereafter scaup, consume a variety of aquatic invertebrates, plants, and occasionally small fish. Scaup have foraged on commercial aquaculture farms in the southern United States for decades. However, the types, abundance, and rate of fish exploitation by scaup on baitfish and sportfish farms are not well documented. Thus, information is needed to understand how fish and other foods influence scaup use of aquatic resources, and any potential economic effects of depredation of fish. From November–March in winters 2016–2017 and 2017–2018, we conducted 1,458 pond surveys to estimate …

Impact Of Covid-19 On The Us And Texas Economy: A General Equilibrium Approach, Lirong Liu, Steven S. Shwiff, Stephanie A. Shwiff, Maryfrances Miller

United States Department of Agriculture Wildlife Services: Staff Publications

This paper examines the impact of COVID-19 on the US and Texas economy using a computable general equilibrium model, REMI PI+. We consider three scenarios based on economic forecasts from various sources, including the University of Michigan’s RSQE (Research Seminar in Quantitative Economics), IMF, and the Wi orld Bank. We report a GDP loss of $106 million (a 6% decline) with 1.2 million jobs lost (6.6%) in Texas in 2020. At the national level, GDP loss is $996 billion (a 5% decline) with 11.5 million jobs lost (5.5%) in the same year. By 2026, the aggregate total GDP loss in …

Conflict, Coexistence, Or Both? Cougar Habitat Selection, Prey Composition, And Mortality In A Multiple-Use Landscape, David C. Stoner, Mark A. Ditmer, Dustin L. Mitchell, Julie K. Young, Michael L. Wolfe

United States Department of Agriculture Wildlife Services: Staff Publications

Western North America is experiencing remarkable human population growth and land-use change. Irrigation and associated cultivation have led to colonization of urban-wildland interface (UWI) environments by mule deer (Odocoileus hemionus), and consequently, cougars (Puma concolor). In the wake of these changes, human-wildlife conflicts have increased in tandem with questions about long-term species conservation. To address these concerns, we fit 79 cougars with radio-telemetry collars in the Oquirrh Mountains near Salt Lake City, Utah (2002–2010). Our goal was to evaluate variation in cougar habitat selection, diet, and cause-specific mortality in a landscape dominated by urban, military, and industrial activities. We used …

Global Distribution, Host Range And Prevalence Of Trypanosoma Vivax: A Systematic Review And Meta-Analysis, Eyerusalem Fetene, Samson Leta, Fikru Regassa, Philippe Büscher

Harold W. Manter Laboratory of Parasitology: Library Materials

Background

Trypanosomosis caused by Trypanosoma vivax is one of the diseases threatening the health and productivity of livestock in Africa and Latin America. Trypanosoma vivax is mainly transmitted by tsetse fies; however, the parasite has also acquired the ability to be transmitted mechanically by hematophagous dipterans. Understanding its distribution, host range and prevalence is a key step in local and global efforts to control the disease.

Methods

The study was conducted according to the methodological recommendations of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) checklist. A systematic literature search was conducted on three search engines, namely PubMed, …

Visual Growth Tracking For Automated Leaf Stage Monitoring Based On Image Sequence Analysis, Srinidhi Bashyam, Sruti Das Choudhury, Ashok Samal, Tala Awada

School of Computing: Faculty Publications

In this paper, we define a new problem domain, called visual growth tracking, to track different parts of an object that grow non-uniformly over space and time for application in image- based plant phenotyping. The paper introduces a novel method to reliably detect and track individual leaves of a maize plant based on a graph theoretic approach for automated leaf stage monitoring. The method has four phases: optimal view selection, plant architecture determination, leaf tracking, and generation of a leaf status report. The method accepts an image sequence of a plant as the input and automatically generates a leaf status …

Influence Of Irrigation Drivers Using Boosted Regression Trees: Kansas High Plains, Susan E. Lamb, Erin M.K. Haacker, Samuel J. Smidt

Department of Earth and Atmospheric Sciences: Faculty Publications

Groundwater levels across parts of western Kansas have been declining at unsustainable rates due to pumping for agricultural irrigation despite water-saving efforts. Accelerating this decline is the complex agricultural landscape, consisting of both categorical (e.g., management boundaries) and numerical (e.g., crop prices) factors that drive irrigation decisions, making integrated water budget management a challenge. Furthermore, these factors frequently change through time, rendering management strategies outdated within relatively short time scales. This study uses boosted regression trees to simultaneously analyze categorical and numerical data against annual irrigation pumping to determine the relative influence of each factor on groundwater pumping across both …

Honey Bee Foraged Pollen Reveals Temporal Changes In Pollen Protein Content And Changes In Forager Choice For Abundant Versus High Protein Flowers, Gabriela Quinlan, Meghan Milbrath, Clint Otto, Autumn Smart, Deborah D. Iwanowicz, Robert Cornman, Rufus Isaacs

Department of Entomology: Faculty Publications

Protein derived from pollen is an essential component of healthy bee diets. Protein content in honey bee foraged pollen varies temporally and spatially, but the drivers underlying this variation remain poorly characterized. We assessed the temporal and spatial variation in honey bee collected pollen in 12 Michigan apiaries over 3 summers (2015–2017). We simultaneously monitored forage in flowering habitats (uncultivated floristically-rich areas and conservation program land) near these apiaries throughout the growing season. We used these data, along with data from the literature on plant pollen protein content, to determine if honey bees collected a greater proportion of pollen from …

A First Flight In North Carolina—But Not At Kitty Hawk, Robert J. Wright

Department of Entomology: Faculty Publications

The southern corn billbug is a univoltine insect most common in the Coastal Plains region of North Carolina, Virginia, Georgia, and South Carolina. However, this insect can also be found in the midwestern U.S. (Van Duyn and Wright 1999). Early in the growing season, the adults can be found feeding at the base of corn plants—typically with their head pointed downward (Fig. 1)—by inserting their beak into the base of the plant. They can kill or stunt seedlings or injure the developing leaves inside the plant, causing twisted leaves as they emerge or a series of small holes across the …

Transference Of Citizen Science Program Impacts: A Theory Grounded In Public Participation In Scientific Research, Louise I. Lynch-O’Brien, Wayne A. Babchuk, Jenny M. Dauer, Tiffany Heng-Moss, Doug Golick

Department of Entomology: Faculty Publications

Citizen science is known for increasing the geographic, spatial, and temporal scale from which scientists can gather data. It is championed for its potential to provide experiential learning opportunities to the public. Documentation of educational outcomes and benefits for citizen scientists continues to grow. This study proposes an added benefit of these collaborations: the transference of program impacts to individuals outside of the program. The experiences of fifteen citizen scientists in entomology citizen science programs were analyzed using a constructivist grounded theory methodology. We propose the substantive-level theory of transference to describe the social process by which the educational and …

Development And First Evaluation Of An Attractant Impregnated Adhesive Tape Against Blood-Sucking Flies, Junwei J. Zhu, Gwang-Hyun Roh, Yosuke Asamoto, Kujtim Bizati, Jen-Chieh Liu, Alexander Lehmann, Kyle Harrison, David B. Taylor, Hironao Otake

Department of Entomology: Faculty Publications

Stable flies are one of the most important arthropod pests of livestock that reduce cattle weight gain and milk production leading to annual economic losses in excess of $2 billion to the US cattle industry. The host-seeking behavior is primarily mediated by associated odors from stable fly larval development environments and host animals. The present paper reports the development and evaluation of attractant-impregnated adhesive tapes to reduce stable fly attacks on cattle. Laboratory bioassays showed that only m-cresol impregnated adhesive tapes caught significantly more stable flies (16 ± 1) than the control tape without attractant added (7 ± 1), with …

Hitchhiking Or Hang Gliding? Dispersal Strategies Of Two Cereal-Feeding Eriophyoid Mite Species, Agnieszka Majer, Alicja Laska, Gary Hein, Lechosław Kuczyński, Anna Skoracka

Department of Entomology: Faculty Publications

Dispersal shapes the dynamics of populations, their genetic structure and species distribution; therefore, knowledge of an organisms’ dispersal abilities is crucial, especially in economically important and invasive species. In this study, we investigated dispersal strategies of two phytophagous eriophyoid mite species: Aceria tosichella (wheat curl mite, WCM) and Abacarus hystrix (cereal rust mite, CRM). Both species are obligatory plant parasites that infest cereals and are of economic significance. We investigated their dispersal success using different dispersal agents: wind and vectors. We hypothesised that in both mite species the main mode of dispersal is moving via wind, whereas phoretic dispersal is …

A Comprehensive Review On Bioactive Peptides Derived From Milk And Milk Products Of Minor Dairy Species, Snigdha Guha, Heena Sharma, Gaurav Kr Deshwal, Priyanka Singh Rao

Department of Food Science and Technology: Faculty Publications

Milk from different species has been exploited for the isolation of various functional ingredients for decades. Irrespective of the source, milk is considered as a complete food, as it provides essential nutrients required by the human body. Proteins and their fractions are valuable sources of bioactive peptides that might exert a health beneficial role in the human body such as immune-modulation, antioxidant activity, ACE-inhibitory activity, anti- neoplastic, anti-microbial, etc. In milk, bioactive peptides may either be present in their natural form or released from their parental proteins due to enzymatic action. The increasing interest in bioactive peptides among researchers has …