Digital Commons Network^™

In Vivo Printing Of Growth Factor-Eluting Adhesive Scaffolds Improves Wound Healing, Kristo Nuutila, Mohamadmahdi Samandari, Yori Endo, Yuteng Zhang, Jacob Quint, Tannin A. Schmidt, Ali Tamayol, Indranil Sinha

Department of Mechanical and Materials Engineering: Faculty Publications

Acute and chronic wounds affect millions of people around the world, imposing a growing financial burden on patients and hospitals. Despite the application of current wound management strategies, the physiological healing process is disrupted in many cases, resulting in impaired wound healing. Therefore, more efficient and easy-to-use treatment modalities are needed. In this study, we demonstrate the benefit of in vivo printed, growth factor-eluting adhesive scaffolds for the treatment of full-thickness wounds in a porcine model. A custom-made handheld printer is implemented to finely print gelatin-methacryloyl (GelMA) hydrogel containing vascular endothelial growth factor (VEGF) into the wounds. In vitro and …

Novel Scalable And Simplified System To Generate Microglia-Containing Cerebral Organoids From Human Induced Pluripotent Stem Cells, Brittany Bodnar, Yongang Zhang, Jinbiao Liu, Yuan Lin, Peng Wang, Zhengyu Wei, Sami Saribas, Yuanjun Zhu, Fang Li, Xu Wang, Wenli Yang, Qingsheng Li, Wen-Zhe Ho, Wenhui Hu

Nebraska Center for Virology: Faculty Publications

Human cerebral organoid (CO) is a three-dimensional (3D) cell culture system that recapitulates the developing human brain. While CO has proved an invaluable tool for studying neurological disorders in a more clinically relevant matter, there have still been several shortcomings including CO variability and reproducibility as well as lack of or underrepresentation of certain cell types typically found in the brain. As the technology to generate COs has continued to improve, more efficient and streamlined protocols have addressed some of these issues. Here we present a novel scalable and simplified system to generate microglia-containing CO (MCO). We characterize the cell …

Hospital Sketches, Louisa May Alcott

Zea E-Books in American Studies

In November 1862, Louisa May Alcott (1832–1888) signed up as a volunteer nurse for the Sanitary Commission charged with caring for the Civil War’s mounting casualties. From 13 December 1862 until 21 January 1863, Miss Alcott served at the Union Hotel Hospital in Georgetown in the District of Columbia, where she ultimately contracted typhoid and pneumonia and very nearly died. This book is her account of her journey south from Concord and her six weeks in the nation’s wartime capital. Styling herself by the fanciful name “Tribulation Periwinkle,” she brought humor as well as pathos to her subject, making this …

Complete Closed Genome Sequence Of The Extremely Heat- Resistant Strain Escherichia Coli Aw1.7, Manita Guragain, James L. Bono, Joseph M. Bosilevac

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Escherichia coli isolate AW1.7 is an extremely heat-resistant bacterium and has been widely used as a reference strain in extreme heat resistance studies for almost a decade. Here, we report its complete closed genome sequence.

Using Genomics To Measure Phenomics: Repeatability Of Bull Prolificacy In Multiple-Bull Pastures, Gary L. Bennett, John W. Keele, Larry A. Kuehn, Warren M. Snelling, Robert A. Cushman, Tara G. Mcdaneld, Aaron M. Dickey, Darrell Light

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Phenotypes are necessary for genomic evaluations and management. Sometimes genomics can be used to measure phenotypes when other methods are difficult or expensive. Prolificacy of bulls used in multiple-bull pastures for commercial beef production is an example. A retrospective study of 79 bulls aged 2 and older used 141 times in 4–5 pastures across 4 years was used to estimate repeatability from variance components. Traits available before each season’s use were tested for predictive ability. Sires were matched to calves using individual genotypes and evaluating exclusions. A lower-cost method of measuring prolificacy was simulated for five pastures using the bulls’ …

Diverse University Students Across The United States Reveal Promising Pathways To Hunter Recruitment And Retention, Victoria R. Vayer, Lincoln R. Larson, M. Nils Peterson, Kangjae Jerry Lee, Richard Von Furstenberg, Daniel Y. Choi, Kathryn Stevenson, Adam A. Ahlers, Christine Anhalt-Depies, Taniya Bethke, Jeremy Bruskotter, Christopher J. Chizinski, Brian Clark, Ashley A. Dayer, Benjamin Ghasemi, Larry Gigliotti, Alan Graefe, Kris Irwin, Samuel J. Keith, Matt Kelly, Gerard Kyle, Elizabeth Metcalf, Wayde Morse, Mark D. Needham, Neelam Poudyal, Michael Quartuch, Shari Rodriguez, Chelsie Romulo, Ryan L. Sharp, William Siemer, Matt Springer, Richard Stedman, Taylor Stein, Tim Van Deelen, Jason Whiting, Richelle L. Winkler, Kyle Maurice Woosnam

School of Natural Resources: Faculty Publications

Declining participation in hunting, especially among young adult hunters, affects the ability of state and federal agencies to achieve goals for wildlife management and decreases revenue for conservation. For wildlife agencies hoping to engage diverse audiences in hunter recruitment, retention, and reactivation (R3) efforts, university settings provide unique advantages: they contain millions of young adults who are developmentally primed to explore new activities, and they cultivate a social atmosphere where new identities can flourish. From 2018 to 2020, we surveyed 17,203 undergraduate students at public universities across 22 states in the United States to explore R3 potential on college campuses …

Center For Grassland Studies, July 2021

Center for Grassland Studies: Newsletters

Contents

21st Nebraska Grazing Conference on the Horizon by Daren Redfearn

Center for Grassland Studies Policy Advisory Committee

Director's Column by Walt Schacht (Interim Director)

21st Annual Nebraska Grazing Conference Schedule, August 9-11, 2021

Club Officers Elected for FY 21-22 by Jessica Windh

2021 Fall Seminar Series Schedule

CGS Scholarships and Fellowship Awarded for FY 21-22

Congratulations May 2021 Graduates

Production Performance Of Cows Raised With Different Postweaning Growth Patterns, Harvey C. Freetly, Robert A. Cushman, Gary L. Bennett

Roman L. Hruska U.S. Meat Animal Research Center: Reports

The period of heifer development is a relatively small fraction of a cow's life; however, her pattern of growth may have permanent effects on her productivity as a cow. We hypothesized that altering the growth pattern during the peri-pubertal period would increase life-time productivity across genetic types of Bos taurus cows. The objective was to determine the stayability, calf production, and weight of calf weaned across six calf crops. Heifers (n = 685) were placed on one of two developmental programs at 256 ± 1 d of age. Control heifers received a diet that provided 228 kcal ME·(body weight [BW], …

Afterschool Sustainability Club, Ethan Halman Gonzalez

Honors Program: Expanded Learning Clubs

In-depth lesson plans used at Riley Elementary to educate students about the environment around them and how to preserve it.

The Usda-Ars Ag100pest Initiative: High-Quality Genome Assemblies For Agricultural Pest Arthropod Research, Anna K. Childers, Scott M. Geib, Sheina B. Sim, Monica F. Poelchau, Brad S. Coates, Tyler J. Simmonds, Erin D. Scully, Timothy P. L. Smith, Christopher P. Childers, Renee L. Corpuz, Kevin Hackett, Brian Scheffler

Roman L. Hruska U.S. Meat Animal Research Center: Reports

The phylum Arthropoda includes species crucial for ecosystem stability, soil health, crop production, and others that present obstacles to crop and animal agriculture. The United States Department of Agriculture’s Agricultural Research Service initiated the Ag100Pest Initiative to generate reference genome assemblies of arthropods that are (or may become) pests to agricultural production and global food security. We describe the project goals, process, status, and future. The first three years of the project were focused on species selection, specimen collection, and the construction of lab and bioinformatics pipelines for the efficient production of assemblies at scale. Contig-level assemblies of 47 species …

Nebline, July 2021

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Smart Techniques Conserve Water in Your Yard

Building a Healthy Community: What Lincolnites Say

Recipe of the Month

Saving and Sing and Storing Garden Seeds

Garden Guide: Things to Do This Month

Naturally Occurring Elements in Groundwater: Last in a Series - Uranium

UNL East Campus Discovery Days

Nebraska 4rs Nutrient Stewardship Field Day

Ants in the Home & Landscape

Lawn Care and Young Children: Creating a Safe and Fun Place to Play

Heart of 4-H Volunteer Award: Marti Thomson

Summer Programming 4-H Interns

4-H Announcements for 4H'ers and Volunteers

Extension News

Extension Calendar

Wpa News 115 (2021), World Pheasant Association

Galliformes Specialist Group and Affiliated Societies: Newsletters

WPA News (Summer 2021), number 115

Published by the World Pheasant Association

Model-Based Localization Of Deep-Diving Cetaceans Using Towed Line Array Acoustic Data, Yvonne M. Barkley, Eva-Marie Nosal, Eerin M. Oleson

United States Department of Commerce: Staff Publications

Passive acoustic monitoring using a towed line array of hydrophones is a standard method for localizing cetaceans during line-transect cetacean abundance surveys. Perpendicular distances estimated between localized whales and the trackline are essential for abundance estimation using acoustic data. Uncertainties in the acoustic data from hydrophone movement, sound propagation effects, errors in the time of arrival differences, and whale depth are not accounted for by most two-dimensional localization methods. Consequently, location and distance estimates for deep-diving cetaceans may be biased, creating uncertainty in abundance estimates. Here, a model-based localization approach is applied to towed line array acoustic data that incorporates …

Water Current, Volume 53, No. 1, Summer 2021

Water Current Newsletter

No abstract provided.

Droughtscape, Summer 2021, National Drought Mitigation Center

DroughtScape: Quarterly Newsletter of the National Drought Mitigation Center (2007–2023)

DroughtScape, Summer 2021

Understanding The Roles Of Nutrient-Niche Dynamics In Clostridioides Difficile Colonization In Human Microbiome Colonized Minibioreactors, Xiaoyun Huang

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Clostridioides difficile is a Gram-positive pathogen that is one of the most common causes of health care-associated infections in the U.S. Because of its importance to human health, researchers are trying to understand risk factors for infection and identify new therapies to treat disease. A “healthy” gut microbiome has the ability to protect the host against colonization of C. difficile or other pathogens by providing colonization resistance. One proposed mechanism for colonization resistance is through competition for nutrients with pathogens by the members of the healthy microbiome. We tested whether competition for nutrients was important for colonization resistance in human …

The Differences Of Prokaryotic Pan-Genome Analysis On Complete Genomes And Simulated Metagenome-Assembled Genomes, Tang Li

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Metagenomic assembly is often used in microbiome research. In metagenomic assembly, contigs are binned based on the shared nucleotide composition. These contig bins are called metagenome-assembled genomes (MAGs), each representing a unique bacterial genome recovered from metagenome sequencing. Hundreds of thousands of high-quality MAGs of various ecological environments have been published since 2017, and increasingly more MAGs are being used in pan-genome analyses where unculturable species or species without reference genomes are studied in microbiome research. However, compared to the traditional pan-genome analysis that uses isolate genomes (from a pure strain isolated from a mixed bacterial population), it is not …

Protective Role Of Palmitoleate Against Zika Virus-Induced Apoptosis In Placental Trophoblasts, Philma Glora Muthuraj

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Zika virus (ZIKV) infection in pregnant women causes congenital Zika syndrome which involves birth defects such as intrauterine growth restriction (IUGR), retinal defects and microcephaly in the fetus. ZIKV infection results in placental pathology which plays a crucial role in disease transmission from mother to fetus. Currently, there is no Food and Drug Administration (FDA) approved vaccine or therapeutic drug against ZIKV. In this thesis, we have elucidated the cell signaling pathways connected to ZIKV-induced apoptosis in trophoblasts and also the protective effect of palmitoleate against ZIKV-induced apoptosis. First, we demonstrated the molecular mechanism behind ZIKV-induced apoptosis using an in …

Examining The Effects Of The Write Sounds Intervention With First Grade Students, Brittany Wambold

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Students who have difficulty with reading and writing are at risk to continue having difficulty throughout their schooling. Lack of time and resources may be a contributing factor for students not receiving additional instruction for both skills. However, there is evidence that balanced reading and writing programs can be effective. The purpose of this study was to examine the effectiveness of the Write Sounds intervention for students who had deficits in reading and writing. This study was a multiple baseline across participants design with three first-grade students who showed difficulty with reading, spelling, and phonemic awareness. Students received 40 minutes …

Navigating Non-Physical Borders: An Examination Of The Boundaries Of Exclusion And Ideas About Inclusion Of Immigrants In American Communities, Alison O'Toole

Department of Political Science: Dissertations, Theses, and Student Research

Are exclusionary boundaries drawn by those who aren’t accepting of immigrants malleable? Do beliefs about inclusion on the part of those who tend to be more accepting toward immigrants have limits? To address these questions, I look at the major factors that I believe influence reactions to immigrants: national identity and trust, and values. This dissertation contributes to two important goals. The first is to help ensure that long- term residents in communities accept people from diverse cultures and backgrounds. The second is softening the divisive power of the immigration issue to make it less of a staple in the …

Understanding And Contextualizing Foraging Among Recreational Opportunities In The North Central United States, Iris I. Mcfarlin

School of Natural Resources: Dissertations, Theses, and Student Research

Over the past few decades, there has been a resurgence in popularity and recognition of foraging for wild products and foods. Despite the cultural importance and ubiquity of foraging, there have been relatively few scientific investigations (as compared to other consumptive outdoor activities such as hunting and fishing) of the social factors influencing foraging behavior, landscape preferences, and the types of materials foraged in the United States. As such, there is a fundamental need to understand more about the practice and about those who participate. We conducted two surveys to gather information on foragers’ motivations and demographic characteristics and to …

Cover Guys: Masculinity, Sexuality, And Representations Of Men's Bodies In Popular Magazines For Men, Trenton M. Haltom

Department of Sociology: Dissertations, Theses, and Student Research

Scant research puts magazines into conversation with sociological theories of masculinity or sexuality. Yet, magazines have long projected idealized images of masculinities, the male body, and men’s sexuality. In this dissertation, I examine representations of men in popular magazines, highlighting the multifaceted ways magazines have marketed masculinity and the sexualization of men.

Using an explanatory sequential mixed method content analysis, I analyze 38 years (1980–2018; N=2,750) of magazine covers from GQ (n=516), Men’s Health (n=277), and Sports Illustrated (n=1,671). Each cover was coded using a standardized coding form developed for this dissertation. The coding …

Targeted Violence On Campus: A Comparison Of Exposure And Response To Bias And Otherwise Motivated Potential Pre-Incident Behavior On A College Campus, Kyle Siddoway

Department of Psychology: Dissertations, Theses, and Student Research

Acts of targeted violence are of great concern to college administrators. Additionally, targeted violence motivated by bias (e.g., racism, sexism, homophobia, xenophobia, etc.) is occurring at an increasing rate on campuses across the country. Previous research has identified potential pre-incident behaviors which may serve as indicators that an individual is escalating towards violent action. However, very limited research has been conducted which examines pre-incident behaviors which occur in bias motivated violence or aggression. With an undergraduate population (n = 1342), this study utilized a survey asking about exposure and response to both otherwise and biased motivated potential pre-incident behaviors on …

Range-Wide Declines Of Northern Spotted Owl Populations In The Pacific Northwest: A Meta-Analysis, Alan B. Franklin, Katie M. Dugger, Damon B. Lesmeister, Raymond J. Davis, J. David Wiens, Gary C. White, James D. Nichols, James E. Hines, Charles B. Yackulic, Carl J. Schwarz, Steven H. Ackers, L. Steven Andrews, Larissa L. Bailey, Robin Bown, Jesse Burgher, Kenneth P. Burnham, Peter C. Carlson, Tara Chestnut, Mary M. Conner, Krista E. Dilione, Eric D. Forsman, Elizabeth M. Glenn, Scott A. Gremel, Keith A. Hamm, Dale R. Herter, J. Mark Higley, Rob B. Horn, Julianna M. Jenkins, William L. Kendall, David W. Lamphear, Christopher Mccafferty, Trent L. Mcdonald, Janice A. Reid, Jeremy T. Rockweit, David C. Simon, Stan G. Sovern, James K. Swingle, Heather Wise

United States Department of Agriculture Wildlife Services: Staff Publications

The northern spotted owl (Strix occidentalis caurina) inhabits older coniferous forests in the Pacific Northwest and has been at the center of forest management issues in this region. The immediate threats to this federally listed species include habitat loss and competition with barred owls (Strix varia), which invaded from eastern North America. We conducted a prospective meta-analysis to assess population trends and factors affecting those trends in northern spotted owls using 26 years of survey and capture-recapture data from 11 study areas across the owls' geographic range to analyze demographic traits, rates of population change, and occupancy parameters for spotted …

Development Of Vaccines And Antivirals Against Zika Virus, Aryamav Pattnaik

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

The re-emergence of Zika virus (ZIKV), an arbovirus, poses a major global human health concern because of its ability to cause congenital abnormalities and neurological diseases. While many candidate vaccines and antiviral drugs are in the developmental pipeline, none have been approved for use against ZIKV infection. This dissertation describes the characterization of one vaccine and two antiviral drug candidates against ZIKV infection. A bacterial ferritin-based nanoparticle vaccine, termed zDIII-F, is designed to display multiple copies of ZIKV E protein domain III on its surface. These stable nanoparticles are shown to induce robust antibody-mediated protection against lethal ZIKV infection in …

Determination Of Freedom-From-Rabies For Small Indian Mongoose Populations In The United States Virgin Islands, 2019–2020, A. Springer Browne, Hannah M. Cranford, Clint N. Morgan, James A. Ellison, Are Berentsen, Nicholas Wiese, Alexandra Medley, John Rossow, Leanne Jankelunas, Alan S. Mckinley, Claudia D. Lombard, Nicole F. Angeli, Thomas Kelley, Jennifer Valiulus, Bethany Bradford, Valicia J. Burke-France, Cosme J. Harrison, Irene Guendel, Marissa Taylor, Gerard L. Blanchard, Jeffrey B. Doty, David J. Worthington, David Horner, Keith R. Garcia, Joseph Roth, Brett R. Ellis, Kristine M. Bisgard, Ryan Wallace, Esther M. Ellis

United States Department of Agriculture Wildlife Services: Staff Publications

Mongooses, a nonnative species, are a known reservoir of rabies virus in the Caribbean region. A cross-sectional study of mongooses at 41 field sites on the US Virgin Islands of St. Croix, St. John, and St. Thomas captured 312 mongooses (32% capture rate). We determined the absence of rabies virus by antigen testing and rabies virus exposure by antibody testing in mongoose populations on all three islands. USVI is the first Caribbean state to determine freedom-from-rabies for its mongoose populations with a scientifically-led robust cross-sectional study. Ongoing surveillance activities will determine if other domestic and wildlife populations in USVI are …

Testing A Bite-Shortened Hook To Minimize Fish Handling In A Recreational Fishery, Holden E. Harris, Brian K. Whalen, Andrew G. Gude, Michael S. Allen

United States Department of Agriculture Wildlife Services: Staff Publications

Discard mortality can make fishing unsustainable, even in catch-and-release or highly regulated fisheries. However, fishing practices and gears that minimize hook injury, handling, and air exposure can considerably improve fitness and survival in released fish. This study tested whether modified hooks could allow anglers to successfully land and then release fish in the water and without handling. Standard, barbless, and bite-shortened hooks were used to catch Spotted Seatrout Cynoscion nebulosus (n > 75 per hook type), and logistic models were used to assess the differences in landing success and hook self-release success by hook type and categorical fish size. Average landing …

Development And First Evaluation Of An Attractant Impregnated Adhesive Tape Against Blood-Sucking Flies, Junwei J. Zhu, Gwang-Hyun Roh, Yosuke Asamoto, Jen-Chieh Liu, Alexander Lehmann, Kyle Harrison, David B. Taylor, Hironao Otake

Department of Entomology: Faculty Publications

Stable flies are one of the most important arthropod pests of livestock that reduce cattle weight gain and milk production leading to annual economic losses in excess of $2 billion to the US cattle industry. The host-seeking behavior is primarily mediated by associated odors from stable fly larval development environments and host animals. The present paper reports the development and evaluation of attractant-impregnated adhesive tapes to reduce stable fly attacks on cattle. Laboratory bioassays showed that only m-cresol impregnated adhesive tapes caught significantly more stable flies (16 ± 1) than the control tape without attractant added (7 ± 1), with …

Evidence That Toxin Resistance In Poison Birds And Frogs Is Not Rooted In Sodium Channel Mutations And May Rely On “Toxin Sponge” Proteins, Fayal Abderemane-Ali, Nathan D. Rossen, Megan E. Kobiela, Robert A. Craig Ii, Catherine E. Garrison, Zhou Chen, Claire M. Colleran, Lauren A. O'Connell, J Du Bois, John P. Dumbacher, Daniel L. Minor Jr

School of Biological Sciences: Faculty Publications

Many poisonous organisms carry small-molecule toxins that alter voltage-gated sodium channel (Na_V) function. Among these, batrachotoxin (BTX) from Pitohui poison birds and Phyllobates poison frogs stands out because of its lethality and unusual effects on Na_V function. How these toxin-bearing organisms avoid autointoxication remains poorly understood. In poison frogs, a NaV DIVS6 pore-forming helix N-to-T mutation has been proposed as the BTX resistance mechanism. Here, we show that this variant is absent from Pitohui and poison frog NaVs, incurs a strong cost compromising channel function, and fails to produce BTX-resistant channels in poison frog NaVs. We also …

Stockpiling Versus Composting: Effectiveness In Reducing Antibiotic-Resistant Bacteria And Resistance Genes In Beef Cattle Manure, Zachery R. Staley, Bryan L. Woodbury, Bobbi S. Stromer, Amy Schmidt, Daniel D. Snow, Shannon Bartelt-Hunt, Bing Wang, Xu Li

Department of Civil and Environmental Engineering: Faculty Publications

Manure storage methods can affect the concentration and prevalence of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in cattle manure prior to land application. The objective of this study was to compare stockpiling and composting with respect to their effectiveness in reducing ARB and ARGs in beef cattle manure in a field-scale study. Field experiments were conducted in different seasons with different bulking agents for composting. For both the winter-spring cycle and the summer-fall cycle, ARB concentrations declined below the limit of quantification rapidly in both composting piles and stockpiles; however, ARB prevalence was significantly greater in the composting …