Digital Commons Network^™

The Role Of Fatty Acids In Ruminant Diets And Novel Feed Ingredients High In Omega-3 Fatty Acids Fed In Feedlot Diets, Mitchell M. Norman

Department of Animal Science: Dissertations, Theses, and Student Research

A finishing study evaluated the performance, carcass characteristics and fatty acid profiles of steers fed four inclusions of a novel feedstuff Green Grass. Green Grass is a product comprised of sesame meal, giant kelp, cassava, and sorghum containing high concentrations of polyunsaturated fatty acids. No differences were observed in initial BW, final BW, BW gain, HCW, LM area, 12^th rib fat depth, calculated YG, or liver abscess %. Dry matter intake linearly increased as Green Grass inclusion increased in the diet. Steers fed Green Grass had lower G:F than control cattle, and steers fed 30% Green Grass had a …

Low-Oxygen Dry Aging Of Beef, Joseph A. Sonderman

Department of Animal Science: Dissertations, Theses, and Student Research

The objective of this research project was to determine the effects of removing oxygen from the beef dry aging process. Boneless upper 2/3 Choice beef strip loins (n = 18) were randomly assigned to three aging treatments: wet aging, traditional (aerobic) dry aging, and low-oxygen dry aging. All treatments were aged for 41 days at 2 ± 1°C. The dry-aged treatments were held at 50% relative humidity (RH) with a fan speed of 2,200 revolutions per minute (RPM). Low-oxygen dry-aged samples were aged in chambers housed within an oxygen impermeable film bubble. After aging, yield was determined for the …

Estimation Of Breed Effects And Genetic Parameters For Age At Slaughter And Days To Finish In A Multibreed Beef Cattle Population, Lindsay Rae Upperman

Department of Animal Science: Dissertations, Theses, and Student Research

Cattle efficiency during the finishing phase is a crucial factor in determining profit in the beef cattle industry. Economically relevant traits associated with efficient production include age at slaughter (AAS) and days to finish (DtF). Selection to reduce the number of days an animal takes to reach a finish endpoint would ultimately reduce production costs, increase net profits, and result in a more sustainable production system. However, most harvested animals are from commercial herds, necessitating the use of indicator traits from seedstock animals for selection. Potential indicator traits include ultrasound measurements that could be genetically correlated to DtF traits. The …

Afterschool Club: German For K-5, Justin Solheim

Honors Program: Expanded Learning Clubs

The lessons found in this paper were designed for a group of 20-25 elementary schools students in downtown Lincoln, NE. The students were split into two groups: K-2 and 3-5. Through a variety of activities, the students learned the basics of the German language and culture. Many of the lessons are simple and do not require difficult thinking, as this was a summer club. Instead, they allow students to collaborate, move around, color, and solve puzzles.

Nocturnal Multi-Species Roosts Of Cicindelidae (Coleoptera) In A Neotropical Lowland Rainforest, Susan Kirmse, Jürgen Wiesner

Insecta Mundi

Tiger beetles (Coleoptera: Cicindelidae) are frequent predators on the forest floor of the Amazon rainforest. We report on five diurnal sympatric tiger beetle species belonging to the genera Odontocheila Laporte de Castelnau and Poecilochila Rivalier in a terra firme rainforest in South Venezuela. We observed adult beetles for a full year and monitored their nocturnal roosts along two forest paths during the rainy season in 1998. We found up to four species communally roosting on low vegetation along the paths during the night. Multi-species roosts were more often observed than conspecific communal roosts. Although the individual composition of the nocturnal …

The Impacts Of Cover Crops Species And Termination Dates On Arthropod Activity In A Corn Production System, Julia N.D. Campos

Department of Entomology: Dissertations, Theses, and Student Research

Cover crops can reduce soil erosion, increase water infiltration, and provide an alternative strategy for weed management in cropping systems. Cover crops change the landscape composition by increasing plant biodiversity, potentially impacting pests and beneficial insects. Ecological principles suggest that cover crop management can influence the arthropods in the subsequent cash crop. However, the impact of management strategies such as cover crop species and termination date on arthropod activity in corn systems is not well understood. The objective of this study was to determine the impact of grass cover crop species (wheat, cereal rye, and triticale) in combination with four …

Effects Of Cultivars And Nitrogen Management On Wheat Grain Yield And Protein, Deepak Ghimire, Saurav Das, Nathan D. Mueller, Cody F. Creech, Dipak Santra, P Stephen Baenziger, Amanda C. Easterly, Brian Maust, Bijesh Maharjan

Department of Agronomy and Horticulture: Faculty Publications

Low grain protein in hard red winter (HRW) wheat (Triticum aestivum L.) is a serious challenge for rainfed wheat growers, particularly in years with elevated grain yield. Proper nitrogen (N) management with adequate N rate and application timing is critical for optimizing grain yield and protein content. This 2-yr experiment evaluated the effects of different N rates and application timings (fall, spring, and split) on grain yield and protein of two HRW wheat cultivars. Field studies were conducted at four different sites across Nebraska under rainfed conditions in 2018/2019 (Year 1) and 2019/2020 (Year 2). A split plot randomized complete …

Using The Modern Classrooms Project Instructional Model To Address Post-Covid Challenges In The Classroom, Mary Clare Murray

Department of Teaching, Learning, and Teacher Education: Theses and Other Student Research

The trauma and upheaval that students and families suffered due to the Covid pandemic will impact the classroom in the 2021-2022 school year in two significant ways: in the uncertain social-emotional health of students and in the dramatic disparities in their recent learning experiences. What is the most effective method for teachers to address these issues during instruction? The Modern Classrooms teaching method emphasizes self-paced, mastery-based, blended learning that could be a successful method to address post-Covid challenges in the classroom. This paper refers to studies that show the success of each of these instructional aspects: self-paced, mastery-based, and blended …

Pre-Milling Interventions For Improving The Microbiological Quality Of Wheat, Shpresa Musa

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Development of pre-milling intervention strategies to improve the microbial quality of wheat and wheat-based products is an emerging concern for the food industry. When steam tempering conditions for hard and soft wheat were applied, the microbial population associated with the grain was significantly reduced compared to controls. Further reductions were observed when acid was applied as part of the intervention which is an added benefit since the addition of a tempering solution is required for both wheat classes since the desired milling moisture cannot be reached by applying steam alone. On average, the highest reductions for hard wheat were achieved …

Spatiotemporal Trends In Bacterial Diversity Across Three Watersheds Within The Platte River Basin, Nebraska, Esther Perisho

School of Natural Resources: Dissertations, Theses, and Student Research

River bacteria are understudied despite being critical components of river ecosystems. There are even fewer studies considering bacteria communities at large spatiotemporal scales, which may provide insight into drivers of community assembly. We investigated differences in bacterial diversity across environmental gradients within three sub-basins nested in the Platte River Basin, Nebraska. Surface water samples were collected weekly at 36 sites from May to September by the Nebraska Department of Environment and Energy (NDEE) in 2019. Bacterial communities were sequenced using the Illumina MiSeq platform. Sub-basins had similar counts of unique amplicon sequence variants (ASVs) but different community structures. These structural …

Right Side Up: Payment For Ecosystem Services On Privately Owned Grasslands In Nebraska, Kyle Martens

School of Natural Resources: Dissertations, Theses, and Student Research

Grasslands are an important ecological and economic resource in the United States. As part of a natural system, these landscapes can provide income for ranching operations and employment in rural communities; habitat for grassland plants, animals, and migratory species; and offer other services not always readily observed such as improved soil health, clean water, and carbon sequestration. Despite the overarching benefits, the conversion of grasslands to other uses remains widespread throughout much of the remaining Great Plains ecosystem.

Shifting from livestock ranching to another land use often reflects a tipping point. This occurs when the alternative land use is perceived …

Biomaterials With Structural Hierarchy And Controlled 3d Nanotopography Guide Endogenous Bone Regeneration, Shixuan Chen, Hongjun Wang, Valerio Luca Mainardi, Giuseppe Talò, Alec Mccarthy, Johnson V. John, Matthew J. Teusink, Liu Hong, Jingwei Xie

Department of Chemistry: Faculty Publications

Biomaterials without exogenous cells or therapeutic agents often fail to achieve rapid endogenous bone regeneration with high quality. Here, we reported a class of three-dimensional (3D) nanofiber scaffolds with hierarchical structure and controlled alignment for effective endogenous cranial bone regeneration. 3D scaffolds consisting of radially aligned nanofibers guided and promoted the migration of bone marrow stem cells from the surrounding region to the center in vitro. These scaffolds showed the highest new bone volume, surface coverage, and mineral density among the tested groups in vivo. The regenerated bone exhibited a radially aligned fashion, closely recapitulating the scaffold’s architecture. The organic …

Metabolic Modeling And Omics-Integrative Analysis Of Single And Multi-Organism Systems: Discovery And Redesign, Mohammad Mazharul Islam

Department of Chemical and Biomolecular Engineering: Dissertations, Theses, and Student Research

Computations and modeling have emerged as indispensable tools that drive the process of understanding, discovery, and redesign of biological systems. With the accelerating pace of genome sequencing and annotation information generation, the development of computational pipelines for the rapid reconstruction of high-quality genome-scale metabolic networks has received significant attention. These models provide a rich tapestry for computational tools to quantitatively assess the metabolic phenotypes for various systems-level studies and to develop engineering interventions at the DNA, RNA, or enzymatic level by careful tuning in the biophysical modeling frameworks. in silico genome-scale metabolic modeling algorithms based on the concept of optimization, …

Genetic Parameter Estimates For Bull Prolificacy And Its Relationship With Scrotal Circumference In A Commercial Beef Cattle Population, Chad A. Russell, E John Pollak, Matthew L. Spangler

Department of Animal Science: Faculty Publications

The commercial beef cattle industry relies heavily on the use of natural service sires. When artificial insemination is deemed difficult to implement, multisire breeding pastures are used to increase reproductive rates in large breeding herds or to safe-guard against bull injury during the breeding season. Although each bull might be given an equal opportunity to produce offspring, evidence suggest that there is substantial variation in the number of calves sired by each bull in a breeding pasture. With the use of DNA-based paternity testing, correctly assigning calves to their respective sires in multisire pastures is possible and presents an opportunity …

U.S. Geological Survey Science For The Wyoming Landscape Conservation Initiative—2018 Annual Report, Patrick J. Anderson, Cameron L. Aldridge, Jason S. Alexander, Timothy J.` Assal, Steven Aulenbach, Zachary H. Bowen, Anna D. Chalfoun, Geneva W. Chong, Holly Copeland, David R. Edmunds, Steve Germaine, Tabitha Graves, Julie A. Heinrichs, Collin G. Homer, Christopher G. Huber, Aaron N. Johnston, Matthew J. Kauffman, Daniel J. Manier, Ryan R. Mcshane, Cheryl A. Eddy-Miller, Kirk A. Miller, Adrian P. Monroe, Michael S. O'Donnell, Anna Ortega, Annika W. Walters, Daniel J. Wieferich, Teal B. Wyckoff, Linda Zeigenfuss

United States Geological Survey: Water Reports and Publications

The Wyoming Landscape Conservation Initiative (WLCI) was established in 2007 as a collaborative interagency partnership to develop and implement science-based conservation actions. During the past 11 years, partners from U.S. Geological Survey (USGS), State and Federal land management agencies, universities, and the public have collaborated to implement a long-term (more than 10 years) science-based program that assesses and enhances the quality and quantity of wildlife habitats in the southwest Wyoming region while facilitating responsible development. The USGS WLCI Science Team completes scientific research and develops tools that inform and support WLCI partner planning, decision making, and on-the-ground management actions.

In …

Bovine Mammary Alveolar Mac-T Cells Afford A Tool For Studies Of Bovine Milk Exosomes In Drug Delivery, Mojisola Ogunnaike

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Bovine milk exosomes (BMEs) have attracted attention as vehicles for delivering RNA therapeutics. BMEs originate in mammary alveolar cells. Here, we determined whether bovine mammary alveolar MAC-T cells are a promising tool to assess RNA delivery by BMEs. MAC-T cells exosomes (MAC-T BMEs) and BMEs were harvested by differential ultracentrifugation. Exosome size, morphology, microRNA content and marker proteins were assessed using nanoparticle tracking analysis, transmission electron microscopy, real-time PCR and immunoblot analysis, respectively. MAC-T cells were genetically engineered to secrete MAC-T BMEs endogenously labeled with a near-infrared fluorescent protein and tissue distribution was compared to fluorophore-labeled BMEs following intravenous injection …

Alcohol Use And History Of Traumatic Brain Injury In College Students: An Analysis Of Neuropsychological And Behavioral Factors, Jessie Tibbs

Department of Psychology: Dissertations, Theses, and Student Research

Approximately 69,000,000 individuals sustain TBIs each year worldwide. Alcohol use is a known risk factor for TBI. Nearly 50% of individuals with TBI endorse binge drinking, alcohol-related negative consequences, or meet criteria for AUDs. One of the most pressing issues within the alcohol-TBI literature is predicting post-TBI alcohol-related problems, as this results in poorer recovery, rehabilitation, and functioning. Evidence for TBIs causing post-injury alcohol use in those without pre-injury problematic alcohol use is mixed; not all TBI survivors experience alcohol-related problems post-injury. Evidence is mounting that one of the most robust predictors of post-injury alcohol problems is childhood or adolescent …

An Assessment Of The Factors Influencing The Prediction Accuracy Of Genomic Prediction Models Across Multiple Environments, Sarah Widener, George Graef, Alexander E. Lipka, Diego Jarquin

Department of Agronomy and Horticulture: Faculty Publications

The effects of climate change create formidable challenges for breeders striving to produce sufficient food quantities in rapidly changing environments. It is therefore critical to investigate the ability of multi-environment genomic prediction (GP) models to predict genomic estimated breeding values (GEBVs) in extreme environments. Exploration of the impact of training set composition on the accuracy of such GEBVs is also essential. Accordingly, we examined the influence of the number of training environments and the use of environmental covariates (ECs) in GS models on four subsets of n = 500 lines of the soybean nested association mapping (SoyNAM) panel grown in …

Ruminant Milk-Derived Extracellular Vesicles: A Nutritional And Therapeutic Opportunity?, Siew Ling Ong, Cherie Blenkiron, Stephen Haines, Alejandra Acevedo-Fani, Juliana A.S. Leite, Janos Zempleni, Rachel C. Anderson, Mark J. Mccann

Department of Nutrition and Health Sciences: Faculty Publications

Milk has been shown to contain a specific fraction of extracellular particles that are reported to resist digestion and are purposefully packaged with lipids, proteins, and nucleic acids to exert specific biological effects. These findings suggest that these particles may have a role in the quality of infant nutrition, particularly in the early phase of life when many of the foundations of an infant’s potential for health and overall wellness are established. However, much of the current research focuses on human or cow milk only, and there is a knowledge gap in how milk from other species, which may be …

A Smartphone App For Individual Xylazine/Ketamine Calculation Decreased Anesthesia-Related Mortality In Mice, Carlos Poblete Jara, Rodrigo S. Carraro, Ariane Zanesco, Beatriz Andrade, Karina Moreira, Guilherme Nogueira, Bruno L. Souza, Thais Paulino Prado, Valeria Povoa, William Velander, Licio A. Velloso, Eliana P. Araujo

Department of Chemical and Biomolecular Engineering: Faculty Publications

Currently, experimental animals are widely used in biological and medical research. However, the scientific community has raised several bioethical concerns, such as the number of animals required to achieve reproducible and statistically relevant results. These concerns involve aspects related to pain, discomfort, and unwanted animal loss. Retrospectively, we compare two different approaches for anesthesia dosage: a mobile app for dose calculation and a standard dose calculation. A total of 939 C57BL/6J and Swiss mice were analyzed. We collected data on intraoperative and anesthesia-related mortality as described in electronic or physical handwritten records. Our results showed that the mobile app approach …

Generalist Bird Exhibits Site-Dependent Resource Selection, Samantha M. Cady, Craig A. Davis, Samuel D. Fuhlendorf, Rheinhardt Scholtz, Daniel R. Uden, Dirac Twidwell

Department of Agronomy and Horticulture: Faculty Publications

Quantifying resource selection (an organism's disproportionate use of available resources) is essential to infer habitat requirements of a species, develop management recommendations, predict species responses to changing conditions, and improve our understanding of the processes that underlie ecological patterns. Because study sites, even within the same region, can differ in both the amount and the arrangement of cover types, our objective was to determine whether proximal sites can yield markedly different resource selection results for a generalist bird, northern bobwhite (Colinus virginianus). We used 5 years of telemetry locations and newly developed land cover data at two, geographically distinct but …

Lrp-Feeder Cattle Insurance Usage Up With Recent Changes, Jay Parsons

Cornhusker Economics

Market uncertainties pose a significant risk to cattle producers, who have a substantial amount of money invested in breeding livestock, land, and other infrastructure. Live-stock Risk Protection (LRP) insurance became available in the early 2000s from the USDA Risk Management Agency to provide cattle and swine producers with a simple price risk management tool to protect against unexpected down-swings in the national market price. LRP is a single-peril insurance product that provides an indemnity to insured producers if a national price index falls below a selected coverage price on the end date of the policy. LRP insurance functions like a …

Oxygen Reactive Polymers For Treatment Of Traumatic Brain Injury, Parick S. Stayton, Menko P. Ypma, Peter A. Chiarelli, Joshua Sang Hun Park, Richard G. Ellenbogen, Julia Mengyun Xu, Pierre D. Mourad, Donghoon Lee, Anthony Convertime, Forrest M. Kievit

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Methods and compositions for treating traumatic brain injury . The methods and compositions utilize a multi - functional oxygen reactive polymer ( ORP ) that includes repeating units that include a reactive oxygen species ( ROS ) scavenging group and a polyalkylene oxide group . For theranostic applications , the oxygen reactive polymer fur ther includes a diagnostic group .

Developing Pandemic Comics For Youth Audiences, Judy Diamond, Amy Spiegel, Patricia Wonch Hill, Elizabeth Vanwormer, Judi M. Gaiashkibos, Bob Hall, Aaron Sutherlen, Julia Mcquillan

University of Nebraska State Museum: Staff and Affiliates, Publications

In spring 2020 our team received funding from the Rapid Response Research program of the National Science Foundation to develop comics that would help youth understand the COVID-19 pandemic. Our project built on a decade of expertise creating comics about the biology of viruses. In collaboration with virologists and artists, we developed three comic stories about COVID-19 during the pandemic and posted them on-line during the last half of 2020. The fictional narratives address fundamental issues in biology, virology, and network science, in order to help readers understand the complexities of living through a viral pandemic. The stories focus on …

Data From A Public–Industry Partnership For Enhancing Corn Nitrogen Research, C J. Ransom, J D. Clark, G M. Bean, C J. Bandura, M Shafer, N R. Kitchen, J J. Camberato, P R. Carter, R B. Ferguson, F G. Fernandez, D W. Franzen, C.A.M. Laboski, D B. Myers, E D. Nafziger, J E. Sawyer, J F. Shanahan

Department of Agronomy and Horticulture: Faculty Publications

Improving corn (Zea mays L.) N management is pertinent to economic and environmental objectives. However, there are limited comprehensive data sources to develop and test N fertilizer decision aid tools across a wide geographic range of soil and weather scenarios. Therefore, a public-industry partnership was formed to conduct standardized corn N rate response field studies throughout the U.S. Midwest. This research was conducted using a standardized protocol at 49 site-years across eight states over the 2014 to 2016 growing seasons with many soil, plant, and weather related measurements. This note provides the data (found in supplemental files), outlines the data, …

Thermal And Mechanical Analysis Of Bombyx Mori Silk Nanofibers, Justin Busnot

Department of Mechanical and Materials Engineering: Dissertations, Theses, and Student Research

This thesis presents a study on the thermomechanical properties of Bombyx Mori silk nanofibers. These nanofibers were obtained from silkworm cocoons which were degummed to separate the fibroin and the sericin, the two proteins that make up silk. The fibroin was then centrifuged to remove insoluble particles and stored and 4°C before the electrospinning process. A parametric study of the electrospinning process was carried out in order to identify the factors allowing to obtain optimal mechanical properties. The current as well as the flow rate applied, the diameter of the syringe, the distance separating the syringe from collector or even …

Soil Greenhouse Gas Responses To Biomass Removal In The Annual And Perennial Cropping Phases Of An Integrated Crop Livestock System, Elizabeth Christenson, Virginia L. Jin, Marty R. Schmer, Robert B. Mitchell, Daren D. Redfearn

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Diversifying agronomic production systems by combining crops and livestock (i.e., Integrated Crop Livestock systems; ICL) may help mitigate the environmental impacts of intensive single-commodity production. In addition, harvesting row-crop residues and/or perennial biomass could increase the multi-functionality of ICL systems as a potential source for second-generation bioenergy feedstock. Here, we evaluated non-CO2 soil greenhouse gas (GHG) emissions from both row-crop and perennial grass phases of a field-scale model ICL system established on marginally productive, poorly drained cropland in the western US Corn Belt. Soil emissions of nitrous oxide (N2O) and methane (CH4 ) were measured during the 2017–2019 growing seasons …

Cerebrum, Liver, And Muscle Regulatory Networks Uncover Maternal Nutrition Effects In Developmental Programming Of Beef Cattle During Early Pregnancy, Wellison J.S. Diniz, Matthew S. Crouse, Robert A. Cushman, Kyle J. Mclean, Joel S. Caton, Carl R. Dahlen, Lawrence P. Reynolds, Alison K. Ward

Roman L. Hruska U.S. Meat Animal Research Center: Reports

The molecular basis underlying fetal programming in response to maternal nutrition remains unclear. Herein, we investigated the regulatory relationships between genes in fetal cerebrum, liver, and muscle tissues to shed light on the putative mechanisms that underlie the effects of early maternal nutrient restriction on bovine developmental programming. To this end, cerebrum, liver, and muscle gene expression were measured with RNA-Seq in 14 fetuses collected on day 50 of gestation from dams fed a diet initiated at breeding to either achieve 60% (RES, n= 7) or 100% (CON, n= 7) of energy requirements. To build a tissue-to-tissue gene network, we …

Call Repertoire And Inferred Ecotype Presence Of Killer Whales (Orcinus Orca) Recorded In The Southeastern Chukchi Sea, Brijonnay C. Madrigal, Jessica L. Crance, Catherine L. Berchok, Alison K. Stimpert

United States Department of Commerce: Staff Publications

Killer whales occur in the Arctic but few data exist regarding the ecotypes present. The calling behavior differs among ecotypes, which can be distinguished based on pulsed call type, call rate, and bandwidth. In this study, a passive acoustic recorder was deployed 75 km off Point Hope, Alaska, in the southeastern Chukchi Sea to identify which ecotypes were present. A total of 1323 killer whale pulsed calls were detected on 38 of 276 days during the summers (June–August) of 2013–2015. The majority of calls (n = 804, 61%) were recorded in 2013 with the most calls recorded in July …

Distilling Professional Opinion To Gauge Vulnerability Of Guam Avifauna To Brown Treesnake Predation, Robert M. Mcelderry, Eben H. Paxton, Andre V. Nguyen, Shane R. Siers

United States Department of Agriculture Wildlife Services: Staff Publications

The avifauna of Guam was devastated by the introduction of the Brown Treesnake, and the restoration of native birds would need to address the problem with eradication or suppression of BTS. With eradication of the snake unlikely in the near term, and suppression capabilities limited to specific finite areas, key information for reintroductions is how low BTS abundance will likely need to be for each bird species to be re-established based on their vulnerability to BTS predation. Here, we estimate vulnerability, which can no longer be measured directly, so biologists who are familiar with one or more of seven Guam …