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Articles 31831 - 31860 of 38026
Full-Text Articles in Entire DC Network
Ec88-1534 Pest Management Of Farm-Stored Grain, Leroy L. Peters, Ben Doupnik, Richard Pierce
Ec88-1534 Pest Management Of Farm-Stored Grain, Leroy L. Peters, Ben Doupnik, Richard Pierce
University of Nebraska-Lincoln Extension: Historical Materials
Extension circular 88-1534 is about pest management of farm-stored grain.
Ec89-103 Nebraska Fall-Sown Small Grain Variety Tests 1989, Lenis Alton Nelson
Ec89-103 Nebraska Fall-Sown Small Grain Variety Tests 1989, Lenis Alton Nelson
University of Nebraska-Lincoln Extension: Historical Materials
Extension circular 89-103 Nebraska fall-sown small grain variety tests 1989.
Ec88-106 Nebraska Grain Sorghum Hybrid Tests 1988, Lenis Alton Nelson, Roger Wesley Elmore, P. T. Nordquist
Ec88-106 Nebraska Grain Sorghum Hybrid Tests 1988, Lenis Alton Nelson, Roger Wesley Elmore, P. T. Nordquist
University of Nebraska-Lincoln Extension: Historical Materials
Extension circular 88-106 is about Nebraska grain sorghum hybrid tests in 1988.
Ec88-102 Nebraska Spring Small Grain Variety Tests 1988, Lenis Alton Nelson, Russell S. Moomaw, P. Stephen Baenziger
Ec88-102 Nebraska Spring Small Grain Variety Tests 1988, Lenis Alton Nelson, Russell S. Moomaw, P. Stephen Baenziger
University of Nebraska-Lincoln Extension: Historical Materials
Extension circular 88-102 is about Nebraska spring small grain variety tests in 1988.
Annual Report: 1988
Cherry County (Nebraska) Extension: Historical Documents
Includes:
News Clippings
Water Quality in the Home
Horse Packet
Clothing and Textiles
4-H News
Cherry P.I.T.
Homemaker Reflections
Hydrocarbon For Identification And Phenetic Comparisons: Cockroaches, Honey Bees, And Tsetse Flies, David A. Carlson
Hydrocarbon For Identification And Phenetic Comparisons: Cockroaches, Honey Bees, And Tsetse Flies, David A. Carlson
Entomology Papers from Other Sources
The hydrocarbon components of Asian and German cockroaches showed consistent differences by gas chromatography (GC) that did not depend on geographic origin, sex or age, and that did reliably identify individuals of these otherwise morphologically similar species. European honey bee workers and drones showed consistent GC patterns. Race-specific similarities in GC patterns were present in Africanized workers and drones from Central and South America. Principal components analysis separated data from different races. Comb waxes reflected the genetic ancestry of the workers that produced that wax. GC data was used to construct phenetic comparisons of 26 species and subspecies of tsetse …
Adoption Of Newly-Mated Queens: A Mechanism For Proliferation And Perpetuation Of Polygynous Red Imported Fire Ants, B. Michael Glancey, Clifford S. Lofgren
Adoption Of Newly-Mated Queens: A Mechanism For Proliferation And Perpetuation Of Polygynous Red Imported Fire Ants, B. Michael Glancey, Clifford S. Lofgren
Entomology Papers from Other Sources
The polygynous form of the red imported fire ant, Solenopsis invicta Buren was first reported from Mississippi in 1973; however, the source of the numerous fertile queens in polygynous colonies has remained an enigma. In 1987, 400 queens from a mating flight were marked with a durable paint and released in an area heavily populated with the polygynous form. None were recaptured after one week, but 9 months later, 4 clearly-marked queens were found in a nest 65 meters from their release point. This finding clearly points to adoption. About 5 weeks later, 37 of 107 fertile queens collected from …
Use Of Chemical Characters In Defining Populations Of Fire Ants, Robert K. Vander Meer, Clifford S. Lofgren
Use Of Chemical Characters In Defining Populations Of Fire Ants, Robert K. Vander Meer, Clifford S. Lofgren
Entomology Papers from Other Sources
The fire ants, Solenopsis invicta and S. richteri, were accidentally imported into the United States in the first half of this century from South America. In their adopted habitat the imported fire ants have thrived causing considerable medical and agricultural problems in the nine widely infested states of the south and southeast. The red imported fire ant, S. invicta was considered the dominant ant in the infested areas, having displaced the black imported fire ant, S. richteri, into a small enclave in north- eastern Mississippi. However, a large reproductively viable S. invicta/S. richteri hybrid population was recently …
Do Ant Brood Pheromones Exist?, Laurence Morel, Robert K. Vander Meer
Do Ant Brood Pheromones Exist?, Laurence Morel, Robert K. Vander Meer
Entomology Papers from Other Sources
A brood pheromone is a chemical or mixture of chemicals released by immature stages that elicits a behavioral response in conspecific adults. The current literature devoted to brood pheromones in several ant species does not prove the existence of such a pheromone or even of brood-specific chemicals. The possibility of a food response or a nestmate recognition response, two of the most confounding factors, has not been eliminated. Two alternative hypotheses involving brood behavior, morphology, cuticular chemicals, and associative learning are proposed.
Diets Of Darkling Beetles (Coleoptera: Tenebrionidae) Within A Shrub-Steppe Ecosystem, L. E. Rogers, N. E. Woodley, J. K. Sheldon, P. A. Beedlow
Diets Of Darkling Beetles (Coleoptera: Tenebrionidae) Within A Shrub-Steppe Ecosystem, L. E. Rogers, N. E. Woodley, J. K. Sheldon, P. A. Beedlow
Entomology Papers from Other Sources
The diets of 13 species of darkling beetles inhabiting shrub-steppe communities of southcentral Washington were analyzed. Forty-seven vascular plant species, including 7 grasses, 34 forbs, and 6 shrubs, were fed upon. Cluster analysis was used to determine the extent of overlap in food habits among beetle species. The beetle species were separated into five food preference groups. Partitioning of food resources may act to refine niche space of these beetles.
Gonotrophic Development In Hypoderma Lineatum And H. Bovis (Diptera: Oestridae), With Notes On Reproductive Capacity, P. J. Scholl, J. Weintraub
Gonotrophic Development In Hypoderma Lineatum And H. Bovis (Diptera: Oestridae), With Notes On Reproductive Capacity, P. J. Scholl, J. Weintraub
Entomology Papers from Other Sources
Gonotrophic development in Hypoderma lineatum (Villers) and Hypoderma bovis (L.) is characterized by initiation and complete growth in larval and pupal stages. Developing oocytes through six stages to the definitive egg are described. Polytrophic ovarian development is modified; only primary and secondary complements of eggs survive and develop simultaneously. The female emerges from the puparium with its life-long complement of eggs fully developed. Capability to mate and immediately oviposit on host cattle is well suited to the short life span of the nonfeeding fly. Mean reproductive capacity of H. lineatum is 516 ± 54 (SD) eggs, significantly higher than the …
Effect Of The Insect Growth Regulator Fenoxycarh On The Ovaries Of Queens Of The Red Imported Fire Ant (Hymenoptera: Formicidae), B. Michael Glancey, W. A. Banks
Effect Of The Insect Growth Regulator Fenoxycarh On The Ovaries Of Queens Of The Red Imported Fire Ant (Hymenoptera: Formicidae), B. Michael Glancey, W. A. Banks
Entomology Papers from Other Sources
The insect growth regulator, fenoxycarb, ethyl [2-(4-phenoxyphenoxy)ethyl] carbamate, causes retrogression of the ovaries of mated queens of the red imported fire ant, Solenopsis invicta Buren. Fenoxycarb completely suppresses growth of the follicular epithelium, nurse cells, and cytoplasm/yolkplasm. Most developing eggs are resorbed and no new workers are produced. Thus, prolonged exposure of the queen to the compound causes death of the entire colony. The ovaries return to normal if the queen is removed from exposure to the chemical.
Ecology, Behavior, And Distribution Of Blattella Asahinai (Orthoptera: Blattellidae) In Central Florida, Richard J. Brenner, R. S. Patterson, P. G. Koehler
Ecology, Behavior, And Distribution Of Blattella Asahinai (Orthoptera: Blattellidae) In Central Florida, Richard J. Brenner, R. S. Patterson, P. G. Koehler
Entomology Papers from Other Sources
The Asian cockroach, Blattella asahinai Mizukubo, a close relative of the German cockroach, B. germanica (L.), has been discovered in over 900 km2 from Tampa to Lakeland, Fla., and is prevalent in citrus groves, wooded areas, lawns, and homes. Differentiation of the two species is best accomplished by the diagnostic behavior of the Asian cockroach-it is crepuscular (sunset) and nocturnal, readily flies, is attracted at sunset to light-colored or brightly lit surfaces, and lives outdoors in large numbers. Analyses of microhabitat composition of 86 trap sites indicate that the principal habitat of the Asian cockroach is leaf litter in …
Hydrocarbon-Based Discrimination Of Three North American Blattella Cockroach Species (Orthoptera: Blattellidae) Using Gas Chromatography, D. A. Carlson, R. J. Brenner
Hydrocarbon-Based Discrimination Of Three North American Blattella Cockroach Species (Orthoptera: Blattellidae) Using Gas Chromatography, D. A. Carlson, R. J. Brenner
Entomology Papers from Other Sources
The identification of various life stages of three species of cockroaches was accomplished by using gas chromatography for the quantitative determination of cuticular hydrocarbon components. Dried and frozen specimens of wild and laboratory samples of the closely related species, Blattella asahinai Mizukubo and B. germanica (L.), were identified by inspection of chromatographic data. The cuticular hydrocarbons of each species were identified by mass spectrometry and found to be quite unlike those of the distantly related B. vaga Hebard. A statistical technique is described that provides a quantitative approach to numerical pattern recognition of chromatograms for identifying Asian and German cockroaches. …
Studies Of The Neural Basis Of Evasive Flight Behavior In Response To Acoustic Stimulation In Heliothis Zea (Lepidoptera: Noctuidae): Motoneuronal Innervation Of Flight Muscles, Edward Orona, Herndon R. Agee
Studies Of The Neural Basis Of Evasive Flight Behavior In Response To Acoustic Stimulation In Heliothis Zea (Lepidoptera: Noctuidae): Motoneuronal Innervation Of Flight Muscles, Edward Orona, Herndon R. Agee
Entomology Papers from Other Sources
The organization of the motoneurons that control the indirect flight muscles of the fore- and hindwings was investigated in the corn earworm, Heliothis zea (Boddie). Cut axons of the nerves supplying the dorsolongitudinal and dorsoventral muscles were infiltrated with cobalt chloride. The cell bodies of the labeled motoneurons are located throughout all thoracic ganglia. Their respective dendritic fields are organized similarly and distributed in a segmented fashion, i.e., the somata and dendrites are restricted to a rather narrow region of the neuropil. The relevance of this organization for the neural basis of behavior is discussed, focusing on the divergent axonal …
Studies Of The Neural Basis Of Evasive Flight Behavior In Response To Acoustic Stimulation In Heliothis Zea (Lepidoptera: Noctuidae): Organization Of The Tympanic Nerves, H. R. Agee, E. Orona
Studies Of The Neural Basis Of Evasive Flight Behavior In Response To Acoustic Stimulation In Heliothis Zea (Lepidoptera: Noctuidae): Organization Of The Tympanic Nerves, H. R. Agee, E. Orona
Entomology Papers from Other Sources
The organization of the tympanic nerve within the thoracic ganglia of Heliothis zea (Boddie) was investigated. Cobalt chloride infiltration of cut axons was used to investigate the central terminations of the tympanic nerves. The axonal terminals of the A2 acoustic cell were confined to the meso-metathoracic ganglia, whereas the Al acoustic and the nonacoustic B cell were found in the thoracic ganglia. The relevance of this organization for neural circuitry of evasive flight behavior to acoustic stimulation is discussed.
Species Distribution And Characterization Studies Of Sex-Limited Esterases Within The Virgifera Group Of Diabrotica (Coleoptera: Chrysomelidae), Susan K. Ruud, I. C. Mcdonald, D. A. Gabrielson, O. A. Johnson
Species Distribution And Characterization Studies Of Sex-Limited Esterases Within The Virgifera Group Of Diabrotica (Coleoptera: Chrysomelidae), Susan K. Ruud, I. C. Mcdonald, D. A. Gabrielson, O. A. Johnson
Entomology Papers from Other Sources
Isozyme studies revealed the existence of an autosomal, male-limited esterase locus (Est-m) in species of the virgifera group of Diabrotica found in the United States. The locus is characterized by the presence of active and null alleles, and particular taxa are monomorphic or polymorphic for the presumptive Est-m variants. Investigations of the properties of an Est-m form present in the western corn rootworm, Diabrotica virgifera virgifera LeConte, produced data to indicate that the enzyme is a carboxylesterase and a glycoprotein with a relative molecular weight of 78,000 ± 8,680 SD. Additional work has shown that Est-m activity is …
Queen Pheromone Production And Its Physiological Correlates In Fire Ant Queens (Hymenoptera: Formicidae) Treated With Fenoxycarb, Martin S. Obin, B. Michael Glancey, W. A. Banks, Robert K. Vander Meer
Queen Pheromone Production And Its Physiological Correlates In Fire Ant Queens (Hymenoptera: Formicidae) Treated With Fenoxycarb, Martin S. Obin, B. Michael Glancey, W. A. Banks, Robert K. Vander Meer
Entomology Papers from Other Sources
Biological assays were used to evaluate the effect of the insect growth regulator fenoxycarb (ethyl (2-p-phenoxyphenoxy) ethyl carbamate) on production of queen-derived dealation inhibitory pheromone and the queen recognition pheromone of Solenopsis invicta Buren. Treated virgin and mated queens weighed less and were less capable of inhibiting dealation by female alates than were nontreated controls. Virgin queens that gained weight during the experimental trials produced more dealation inhibitory pheromone and more queen recognition pheromone than virgin queens that lost weight. Treated virgin queens with fenoxycarb-induced ovary dysfunction inhibited de alation in proportionally fewer alates than treated virgin queens of similar …
Insect Succession In A Stored-Corn Ecosystem In Southeast Georgia, R. T. Arbogast, M. A. Mullen
Insect Succession In A Stored-Corn Ecosystem In Southeast Georgia, R. T. Arbogast, M. A. Mullen
Entomology Papers from Other Sources
Insect populations infesting a small bulk of corn stored in southeastern Georgia were followed for 8 yr by monthly sampling beginning 8 mo after the corn was placed in storage. The species composition of the insect community changed as the corn deteriorated. Nine species became dominant at some stage of succession. Sitotroga cerealella (Olivier) was the dominant species when the first sample was taken. It was succeeded a month later by Oryzaephilus surinamensis (L.), which declined rapidly after 2 mo of dominance and died out during the third year. Cryptolestes ferrugineus (Stephens) was dominant at various times during the first …
Ec88-219 1988 Nebraska Swine Report, Steven M. Neal, Rodger K. Johnson, Roger J. Kittok, Colleen Kelly, Greg Bussler, Franklin Eldridge, Julie Farver-Koenig, Dwane Zimmerman, Donald G. Levis, J.J. Ford, R. K. Christenson, Murray Danielson, J.B. Morgan, Chris R. Calkins, Roger W. Mandigo, M. .. Brumm, David P. Shelton, Larry Bitney, Duane E. Reese, E.R. Peo Jr., J.D. Hancock, A. J. Lewis, William Ahlschwede, Daniel A. Burosh
Ec88-219 1988 Nebraska Swine Report, Steven M. Neal, Rodger K. Johnson, Roger J. Kittok, Colleen Kelly, Greg Bussler, Franklin Eldridge, Julie Farver-Koenig, Dwane Zimmerman, Donald G. Levis, J.J. Ford, R. K. Christenson, Murray Danielson, J.B. Morgan, Chris R. Calkins, Roger W. Mandigo, M. .. Brumm, David P. Shelton, Larry Bitney, Duane E. Reese, E.R. Peo Jr., J.D. Hancock, A. J. Lewis, William Ahlschwede, Daniel A. Burosh
University of Nebraska-Lincoln Extension: Historical Materials
This 1988 Nebraska Swine Report was prepared by the staff in Animal Science and cooperating departments for use in the Extension and Teaching programs at the University of Nebraska-Lincoln. Authors from the following areas contributed to this publication: Swine Nutrition, swine diseases, pathology, economics, engineering, swine breeding, meats, agronomy, and diagnostic laboratory. It covers the following areas: breeding, disease control, feeding, nutrition, economics, housing and meats.
Agate Fossil Beds National Monument Fossil Management Plan And Environmental Assessment
Agate Fossil Beds National Monument Fossil Management Plan And Environmental Assessment
United States National Park Service: Publications
Agate Fossil Beds National Monument is in Sioux County, north western Nebraska, T28; R55W, 6thPM. It includes portions of Sections 3 through 10 inclusive, plus a small portion of Section 12 (Stenomylus Quarry) . (APPENDIX A )
Relationships Among Calcium-Dependent Protease, Cathepsins B And H, Meat Tenderness And The Response Of Muscle To Aging, Chris R. Calkins, S. C. Seideman
Relationships Among Calcium-Dependent Protease, Cathepsins B And H, Meat Tenderness And The Response Of Muscle To Aging, Chris R. Calkins, S. C. Seideman
Department of Animal Science: Faculty Publications
This study was conducted to compare the relative importance of Ca-dependent protease (CDP) and cathepsins B and H to meat tenderness and changes in tenderness in response to postmortem cooler aging. Charolais bulls (n = 8) and steers (n = 7) were slaughtered at 15 mo of age, and total activities of CDP-I (a protease with neutral pH optimum that requires micromolar amounts of Ca for activity) and cathepsins B and H (lysosomal proteases with acidic pH optima) were determined within 1 h. Shear-force values were obtained after 1, 3, 6, 9 and 14 d of aging. Data were pooled …
Escape Protein Supplementation Of Yearling Steers Grazing Smooth Brome Pastures, S. J. Anderson, Terry J. Klopfenstein, V. Wilkerson
Escape Protein Supplementation Of Yearling Steers Grazing Smooth Brome Pastures, S. J. Anderson, Terry J. Klopfenstein, V. Wilkerson
Department of Animal Science: Faculty Publications
Two grazing trials utilizing individually supplemented yearling steers were conducted to study the effect of supplemental escape protein on steer performance during the active growth periods, spring and fall, of smooth brome (Bromus inermis). Graded levels (0, .11, .23 and .34 kg x head-1 x d-1) of an equal-protein-basis mixture of bloodmeal and corn gluten meal were offered daily, replacing corn starch, which was used as the negative control. All steers received 582 g supplemental dry matter per day. Supplementation with escape protein improved daily performance in both spring (P<.01) and fall (P<.02). Analysis of pooled data from both trials indicated a linear (P<.01) and quadratic (P<.05) increase in steer performance with increasing level of escape protein in the diet. Analysis of grass samples collected throughout and composited over each trial demonstrated that grass protein was highly degraded in the rumen. Using a modified dacron bag technique, 12-h degradability was found to be 80 to 90% of the potentially digestible protein fraction. Rates of protein degradability were 14 and 11.7%/h. Assuming 5%/h rate of passage, escape protein was calculated to be 9.2 and 13.1% of total protein. As a result of the significant growth response observed above that of the energy-supplemented controls and the high ruminat protein degradabilities of the grass observed in the laboratory, it was concluded that growing ruminants grazing actively growing smooth brome pastures were deficient in metabolizable protein.
Relationship Between Herd Management Practices In The Midwest On Milk And Fat Yield, Jeffrey F. Keown
Relationship Between Herd Management Practices In The Midwest On Milk And Fat Yield, Jeffrey F. Keown
Department of Animal Science: Faculty Publications
A dairy management survey was conducted in fall 1985 and spring 1986 in the nine-state area served by the Midstates Dairy Records Processing Center in Ames, IA. The questionnaire, consisting of 57 questions with 254 possible choices, was used to survey dairy producers on DHI testing in Arkansas, Illinois, Iowa, Kansas, Missouri, Nebraska, North Dakota, Oklahoma, and South Dakota. The questions covered housing pactices, milking equipment and practices, feeding regimens, calf rearing and feeding, feed additives, various management categories, and artificial insemination usage. The survey responses were merged with the 1985 year end rolling herd production averages from the processing …
Relative Economic Values Assigned To Milk, Fat Test, And Type In Pricing Of Bull Semen, J. S. Wilder, L. Dale Van Vleck
Relative Economic Values Assigned To Milk, Fat Test, And Type In Pricing Of Bull Semen, J. S. Wilder, L. Dale Van Vleck
Department of Animal Science: Faculty Publications
Data on 324 Holstein bulls from the July 1983 USDA Sire Summary and Holstein Association type evaluations were analyzed using multiple regression to determine which traits or combination of traits in a sire evaluation are most important in determining the price of a straw of semen. Over all bull studs, type was a major economic influence in determining price of semen. When bulls with outlier prices ($100 or more) were excluded, relative economic values over all buII studs were 4.31:2.63:1 for milk, type, and fat test. For three bull studs with more than 35 bulls with evaluations for milk, type, …
Genetic Groups In An Animal Model, R. A. Westell, R. L. Quaas, L. Dale Van Vleck
Genetic Groups In An Animal Model, R. A. Westell, R. L. Quaas, L. Dale Van Vleck
Department of Animal Science: Faculty Publications
Rules are presented for assigning coefficients to the genetic group portion(s) of the mixed model equations after transformation to solve directly for total genetic value (group plus animal solutions) simultaneously for sires and cows using an animal model. Inclusion of all known relationships seems to reduce the need for groups to account for genetic selection and genetic trend. Migration of animals into a population, however, results in a need for grouping to account for genetic merit of the migrants. Selection of parents on which records are not available also creates a need for grouping. Group solutions represent the average genetic …
Comparisons Of Mitochondrial Dna From The Sibling Species Heterodera Glycines And H. Schachtii, A. D. Radice, Thomas O. Powers, Laurie J. Sandall, R. D. Riggs
Comparisons Of Mitochondrial Dna From The Sibling Species Heterodera Glycines And H. Schachtii, A. D. Radice, Thomas O. Powers, Laurie J. Sandall, R. D. Riggs
Department of Plant Pathology: Faculty Publications
Restriction fragment patterns of mitochondrial DNA from sibling species of cyst nematodes Heterodera glycines and H. schachtii were examined. Fourteen restriction endonucleases recognizing four, five, and six base-pair sequences yielded a total of 90 scorable fragments of which 10% were shared by both species. Mitochondrial genome sizes for H. glycines and H. schachtii were estimated to be 22.5-23.5 kb and 23.0 kb, respectively. A single wild type mitochondrial genome was identified in all populations of H. glycines examined, although other mitochondrial genomes were present in some populations. The H. schachtii genome exhibited 57 scorable fragments, compared with 33 identified in …
Estimation Of Genetic Divergence In Meloidogyne Mitochondrial Dna, Thomas O. Powers, Laurie J. Sandall
Estimation Of Genetic Divergence In Meloidogyne Mitochondrial Dna, Thomas O. Powers, Laurie J. Sandall
Department of Plant Pathology: Faculty Publications
Restriction fragments from purified mitochondrial DNA can be readily detected following rapid end-labeling with [α-32P] nucleoside triphosphates and separation by gel electropboresis. Mitochondrial DNA from 12 populations of Meloidogyne species was digested with 12 restriction enzymes producing more than 60 restriction fragments for each species. The mitochondrial genome of M. arenaria is the most genetically distinct of the four species compared. M. arenaria shows approximately 2.1-3.1% nucleotide sequence divergence from the mitochondrial genomes of M. javanica, M. incognita, and M. hapla. Among the latter three species, interspecific estimates of sequence divergence range from 0.7 to …
Taxonomy, Systematics, And Ecology Of Helminth Parasites Of The Ringed Seal, Phoca Hispida Schreber, In Alaskan Waters, Ann Marie Adams
Taxonomy, Systematics, And Ecology Of Helminth Parasites Of The Ringed Seal, Phoca Hispida Schreber, In Alaskan Waters, Ann Marie Adams
Harold W. Manter Laboratory of Parasitology: Library Materials
The genus Orthosplaochnus Odhner, 1905 (Trematoda: Campulidae) was revised, with eight species recognized. Odhneriella Skriabin, 1915 was placed in synonymy with Orthosplanchnus, and Hadwenius was shown to be valid. Orthosplanchnus rossicus (Skriabin, 1915) (syn. Odhneriella rossica) was redescribed.
Inconsistencies in the diagnosis of the Anophryocephalus Baylis, 1922 (Cestoda: Tetrabothriidae) were discussed, along with the basis for generis revision. The taxonomic histories of certain genera of nematodes (e.g., Contracaecum Railliet and Henry, 1912; Dipetalonema Diesing; 1861; and Pseudoterranova Mosgovoi, 1950) were given to explain their present status.
The helminth-fauna of the ringed seal in the North Pacific was compared …
Roles Of A 67-Kda Polypeptide In Reversal Of Protein Synthesis Inhibition In Heme-Deficient Reticulocyte Lysate, Bansidhar Datta, Debopam Chakrabarti, Ananda L. Roy, Naba K. Gupta
Roles Of A 67-Kda Polypeptide In Reversal Of Protein Synthesis Inhibition In Heme-Deficient Reticulocyte Lysate, Bansidhar Datta, Debopam Chakrabarti, Ananda L. Roy, Naba K. Gupta
Department of Chemistry: Faculty Publications
During heme deficiency in reticulocyte lysates, the heme-regulated protein synthesis inhibitor, HRI, phosphorylates the a subunit of eukaryotic initiation factor 2 (eIF-2) and thus inhibits protein synthesis. Two factors, eIF-2 and a reticulocyte-lysate supernatant factor that we term RF, reverse this inhibition. We now report the following. (i) An active eIF-2 preparation contained, in addition to the three subunits (α,β, and γ), a 67-kDa polypeptide. Pretreatment of eIF-2 with polyclonal antibodies against either isolated a subunit or 67-kDa polypeptide almost completely inhibited the reversal activity. Upon further fractionation, three-subunit eIF-2 and the 67-kDa polypeptide were resolved. Neither the …