Digital Commons Network^™

Genotype Classification Of Moraxella Bovis Using Maldi-Tof Ms Profiles, Hannah G. Olson, John Dustin Loy, Michael L. Clawson, Emily L. Wynn, Matthew M. Hille

School of Veterinary and Biomedical Sciences: Faculty Publications

Moraxella bovis (M. bovis) is regarded as a causative agent of infectious bovine keratoconjunctivitis (IBK), the most common ocular disease of cattle. Recently, whole genome sequencing identified the presence of two distinct genotypes within M. bovis that differ in chromosome content, potential virulence factors, as well as prophage and plasmid profiles. It is unclear if the genotypes equally associate with IBK or if one is more likely to be isolated from IBK lesions. We utilized 39 strains of M. bovis that had previously undergone whole genome sequencing and genotype classification to determine the utility of matrix-assisted laser desorption/ionization …

Intestinal Gas Production By The Gut Microbiota: A Review, Erasme Mutuyemungu, Mukti Singh, Sean Liu, Devin J. Rose

Department of Food Science and Technology: Faculty Publications

In addition to causing embarrassment, intestinal gas can be associated with more serious symptoms. This review provides an overview of gas production by the human gut microbiome and outlines foods associated with intestinal gas. Bacteroides, Ruminococcus, Roseburia, Clostridium, Eubacterium, Desulfovibrio, and Methanobrevibacter are among the most abundant microbes responsible for intestinal gas. More than 99% of intestinal gas is composed of hydrogen, carbon dioxide, and methane, while less than 1% is composed of other odiferous compounds. Food groups associated with intestinal gas include pulses, vegetables, fruits, grains, and, for some individuals, dairy. These foods are …

Isolation And Characterization Of Chi-Like Salmonella Bacteriophages Infecting Two Salmonella Enterica Serovars, Typhimurium And Enteritidis, Addisu D. Teklemariam, Mona G. Alharbi, Rashad R. Al-Hindi, Ibrahim Alotibi, Abdullah A. Aljaddawi, Sheren A. Azhari, Ahmed Esmael

Nebraska Center for Virology: Faculty Publications

Salmonella enterica Serovar Typhimurium and Salmonella enterica Serovar Enteritidis are well-known pathogens that cause foodborne diseases in humans. The emergence of antibiotic-resistant Salmonella serovars has caused serious public health problems worldwide. In this study, two lysogenic phages, STP11 and SEP13, were isolated from a wastewater treatment plant in Jeddah, KSA. Transmission electron microscopic images revealed that both phages are new members of the genus “Chivirus” within the family Siphoviridae. Both STP11 and SEP13 had a lysis time of 90 min with burst sizes of 176 and 170 PFU/cell, respectively. The two phages were thermostable (0 ^◦C …

Environment-Specific Selection Alters Flowering-Time Plasticity And Results In Pervasive Pleiotropic Responses In Maize, Nicole E. Choquette, James B. Holland, Teclemariam Weldekidan, Justine Drouault, Natalia De Leon, Sherry Flint-Garcia, Nick Lauter, Seth C. Murray, Wenwei Xu, Randall J. Wisser

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

• Crop genetic diversity for climate adaptations is globally partitioned. We performed experimental evolution in maize to understand the response to selection and how plant germplasm can be moved across geographical zones.
• Initialized with a common population of tropical origin, artificial selection on flowering time was performed for two generations at eight field sites spanning 25° latitude, a 2800 km transect. We then jointly tested all selection lineages across the original sites of selection, for the target trait and 23 other traits.
• Modeling intergenerational shifts in a physiological reaction norm revealed separate components for flowering-time plasticity. Generalized and local modes of …

Comparative Study Of Differentiating Human Pluripotent Stem Cells Into Vascular Smooth Muscle Cells In Hydrogel-Based Culture Methods, Qing Liu, Zhen Liu, Hongyu Gu, Yuxia Ge, Xuesheng Wu, Fuxing Zuo, Qian Du, Yuguo Lei, Zhanqi Wang, Haishuang Lin

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Vascular smooth muscle cells (VSMCs), which provides structural integrity and regulates the diameter of vasculature, are of great potential for modeling vascular-associated diseases and tissue engineering. Here, we presented a detailed comparison of differentiating human pluripotent stem cells (hPSCs) into VSMCs (hPSCs-VSMCs) in four different culture methods, including 2-dimensional (2D) culture, 3-dimensional (3D) PNIPAAm-PEG hydrogel culture, 3-dimensional (3D) alginate hydrogel culture, and transferring 3- dimensional alginate hydrogel culture to 2-dimensional (2D) culture. Both hydrogel-based culture methods could mimic in vivo microenvironment to protect cells from shear force, and avoid cells agglomeration, resulting in the extremely high culture efficiency (e.g., high …

Exposure Of Egyptian Rousette Bats (Rousettus Aegyptiacus) And A Little Free-Tailed Bat (Chaerephon Pumilus) To Alphaviruses In Uganda, Rebekah C. Kading, Erin M. Borland, Eric C. Mossel, Teddy Nakayiki, Betty Nalikka, Jeremy P. Ledermann, Mary B. Crabtree, Nicholas A. Panella, Luke Nyakarahuka, Amy T. Gilbert, Julian C. Kerbis-Peterhans, Jonathan S. Towner, Brian R. Amman, Tara K. Sealy, Barry R. Miller, Julius J. Lutwama, Robert M. Kityo, Ann M. Powers

United States Department of Agriculture Wildlife Services: Staff Publications

The reservoir for zoonotic o’nyong-nyong virus (ONNV) has remained unknown since this virus was first recognized in Uganda in 1959. Building on existing evidence for mosquito bloodfeeding on various frugivorous bat species in Uganda, and seroprevalence for arboviruses among bats in Uganda, we sought to assess if serum samples collected from bats in Uganda demonstrated evidence of exposure to ONNV or the closely related zoonotic chikungunya virus (CHIKV). In total, 652 serum samples collected from six bat species were tested by plaque reduction neutralization test (PRNT) for neutralizing antibodies against ONNV and CHIKV. Forty out of 303 (13.2%) Egyptian rousettes …

On The Firefly (Coleoptera: Lampyridae) Species Of Carl Linnaeus, Oliver Keller

Insecta Mundi

The firefly species described by Carl Linnaeus in 1758 and 1767 (Coleoptera: Lampyridae) were checked to determine the actual dates of publication. Nine out of twelve species were originally described in 1758 and not in 1767 as published in the majority of firefly literature. Lampyris hespera Linnaeus, 1767 as a junior synonym of Aspisoma lampyris (Linnaeus, 1758) is established.

Se revisaron las especies de luciérnagas descritas por Carl Linnaeus in 1758 y 1767 (Coleoptera: Lampyridae) para la determinación de fechas de publicación. Nueve de las doce especies fueron originalmente descritas en 1758 y no en 1767 como aparece publicado en …

Antimicrobial Efficacy Of A Citric Acid/Hydrochloric Acid Blend, Peroxyacetic Acid, And Sulfuric Acid Against Salmonella On Inoculated Non-Conventional Raw Chicken Products, Emma Nakimera

Department of Food Science and Technology: Dissertations, Theses, and Student Research

The utilization of non-conventional chicken parts as human food varies widely across countries. The high prevalence of Salmonella, especially in the giblets, poses a high risk to public health. Poultry processors have implemented multiple hurdle technology to reduce this gram-negative pathogen in chicken parts. This study was conducted to evaluate the antimicrobial efficacy of a commercial blend of citric acid/ hydrochloric acid (CP), peroxyacetic acid (PAA), and sulfuric acid (SA) in reducing Salmonella inoculated on two chicken giblets: livers and hearts. Chicken hearts and livers were inoculated (6 log CFU/ml of rinsate) by individually immersing them in a cocktail …

Sturgeon Chub Distributional Patterns And Habitat Use And Benthic Fish Assemblage Structure In Missouri River Tributaries Of South Dakota, Mitchell R. Magruder

School of Natural Resources: Dissertations, Theses, and Student Research

Native species of the Missouri River drainage inhabiting benthic habitats dominate state and federal lists of species at risk. Sicklefin Chub Macrhybopsis meeki and Sturgeon Chub Macrhybopsis gelida are two native Missouri River benthic minnows that are currently under review for federal listing under the Endangered Species Act (ESA) due to extensive population declines and local extirpations within their native range. Substantial alterations to the Missouri River threaten Macrhybopsis spp. and other benthic fishes; however, large, less impacted tributaries in South Dakota may act as refugia for native species. The extent of Sicklefin Chub and Sturgeon Chub populations is largely …

Semantic Segmentation Based Deep Learning Approaches For Weed Detection, Puranjit Singh

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Global increase in herbicide use to control weeds has led to issues such as evolution of herbicide-resistant weeds, off-target herbicide movement, etc. Precision agriculture advocates Site Specific Weed Management (SSWM) application to achieve precise and right amount of herbicide spray and reduce off-target herbicide movement. Recent advancements in Deep Learning (DL) have opened possibilities for adaptive and accurate weed recognitions for field based SSWM applications with traditional and emerging spraying equipment; however, challenges exist in identifying the DL model structure and train the model appropriately for accurate and rapid model applications over varying crop/weed growth stages and environment. In our …

Descriptive Dna Barcoding Of Argas (Persicargas) Arboreus And Argas (Persicargas) Persicus Ticks (Ixodida: Argasidae) Infesting Birds In Egypt, Enas H. Ghallab, Ayat Yousery, Mona G. Shaalan

Harold W. Manter Laboratory of Parasitology: Library Materials

Argas ticks are primary parasites of birds with veterinary importance. Nevertheless, these ticks have received little attention regarding molecular identification studies. DNA barcoding is a powerful technique for identifying tick species besides traditional morphological identification. The present work is a first effort to divulge DNA sequences of Argas (Persicargas) arboreus from Egypt and worldwide. We used cytochrome c oxidase subunit I (COI) from A. arboreus infesting herons, and from the fowl tick Argas (Persicargas) persicus. Our results pointed out another success for the Folmer primers that are widely used in DNA barcoding, permitting the discrimination …

Memoirs Of The Foreign Legion, Maurice Magnus, D.H. Lawrence

Zea E-Books Collection

Maurice Magnus was 39 years old when he enlisted in the French Foreign Legion to join the fight against Germany in World War I. Magnus was an American expatriot living in Rome—a theatrical agent, tutor, newspaper correspondent, writer, editor, and literary entrepreneur. He soon discovered his error—the Legion he found consisted largely of German exiles, prison-avoiding felons, and contemptuous French officers. Magnus spent about six weeks training in North Africa before a transfer to southern France provided the opportunity to desert and flee back to Italy. The Memoirs recounts his brief disenchanted tenure as a Legionnaire. After his military service …

Geographic Distribution: Anolis Sagrei (Brown Anole). Usa: Arizona., Addie Leimroth, Louis A. Somma, Dyrana N. Russell, Logan P. Cutts, Mason Ryan, Randall D. Babb, Karen E. Hajek, Andrew T. Holycross

Papers in Herpetology

We discovered the first distributional records for the exotic, Caribbean lizard, Anolis sagrei (Brown Anole), in Arizona.

Interdisciplinary Design Studio: Programming Document Visioning For A Robotic Demonstration, Research, And Engagement Dairy, Steve(N) Hardy, Nate Bicak, Sarah Alduaylij, Noor Al-Maamari, Devyn Beekman, Kelsey Belgum, Lauren Chubb, Nicholas Forte, Mitchell Hill, Joshua Holstein, Dylan Lambe, Phuong Le, Mia Leriger, Elizabeth Loftus, Josh Lorenzen, Megan Lovci, Alex Martino, Zade Miller, Hannah Morgan, Annabelle Nichols, Collin Shearman, Rebecca Sowl, Nalin Theplikhith, Angela Vu, Shaylee Wagner, Ethan Watermeier, Trever Zelenka

Architecture Program: Student Creative Activity

The 2022 COLLABORATE Design Studio brought together students from various disciplines to address a complex, real-world project which required collaborative input from different perspectives. The studio worked to advance the co-creation of knowledge between external stakeholders, students, and instructors. The course was co-taught by faculty from different disciplines, and areas of expertise. During the semester, Nate Bicak and Steven Hardy worked with students from Architecture and Interior Design in collaboration with students in Dr. Tami Brown-Brandl’s students in Biological Systems Engineering and Animal Science to explore the values, spatial qualities, and area requirements of a Robotic Demonstration, Research, and Engagement …

Why Energy Efficiency Is Needed In Rwanda, Jean Ishinzibigwi

Op-Eds from ENSC230 Energy and the Environment: Economics and Policies

Perspective on the benefits of energy efficiency and cost savings on energy was changed by the experience of working with the Nebraska Industrial Assessment Center. Visiting various industrial locations in Nebraska to see how different energy-saving techniques are employed to save money on energy costs and reduce energy waste. Rwanda can put several ideas into action to lessen the energy loss, which is too much now.

The government's Ministry of Infrastructure (MININFRA) and other departments are crucial to Rwanda's energy sector. The primary ministry in charge of developing renewable energy is MININFRA (methane, peat, geothermal, solar and wind energy). The …

Stop Methane Emissions, Jacob Anderson

Op-Eds from ENSC230 Energy and the Environment: Economics and Policies

We all know of the worldly issue called global warming. A huge cause of global warming is the emission and buildup of greenhouse gasses in the atmosphere. Carbon dioxide is the leading contributor to global emissions at 74.4% and methane is second at 17.3% (Our World in Data 2019). On August 16, 2022, President Biden signed the Inflation Reduction Act of 2022

which includes a charge that starts in 2024 at $900 per metric ton of methane emitted and will raise to $1500 by 2028 (CRS). This should introduce an incentive for facilities to update their plant, machinery, and equipment …

Bridgeport Poised To Be Site Of Nebraska’S Next Environmental Catastrophe, Kjersten Hyberger

Op-Eds from ENSC230 Energy and the Environment: Economics and Policies

Bridgeport Ethanol LLC is Nebraska’s first ethanol plant to be under contract for Carbon Capture and Sequestration (CCS) in the state. Despite ongoing safety concerns by federal regulators and organized opposition from affected landowners, Carbon America is pushing forward with their plans to have the Bridgeport CCS facility operational by 2024. At a time when Nebraska has barely begun cleanup of the AltEn disaster in Mead, and the agrochemical contamination levels in our soil and groundwater are at historic levels, we cannot carelessly add one more pollutant to the mix.

In May of this year the Legislature passed the Nebraska …

Big Blue Wind Project: Why Solar May Be A Better Solution For Nebraska's Energy Future Than Wind, Page Nippert

Op-Eds from ENSC230 Energy and the Environment: Economics and Policies

With nonrenewable resources such as coal and oil causing greenhouse gas emissions, a shift to renewable resources is needed not only to reduce CO₂ emissions, but also to allow energy independence. The switch to renewable resources is vital, and in Nebraska, these resources are being introduced commonly as solar and wind farms depending on the area.

NextEra Energy is proposing The Big Blue Wind project in Jefferson County. I’m opposing this project for reasons similar to why it’s being proposed – for the environment and the community.

The project aims to benefit the community economically by creating jobs, county …

Schramm Park Fall Field Days, Janis Paseka

Nebraska Bird Review

The 2022 NOU Fall Field Days were held at Schramm Park State Recreation Area, south of Gretna in Sarpy County, on September 21-23, with approximately 60 in attendance. The meeting had been scheduled and cancelled twice in the two previous years due to coronavirus concerns. The total species count was 119, including a Sandhill Crane, Prairie Falcon (early for away from breeding area), Winter Wren, and early Snow Bunting. A Limpkin, which had been found in late June at Wehrspann Wetlands mitigation pond in Sarpy County and was still seen through late November in the Wehrspann Lake area, was not …

The Nebraska Bird Review, Index To Volume 90

Nebraska Bird Review

From: Abbott, Sydney 56

To: Zonotrichia leucophrys oriantha 66, 146

The Nebraska Bird Review Volume 90 December 2022 Number 4

Nebraska Bird Review

Fall Field Report, August - November 2022 by W. Ross Silcock ...126

Remembering Clem Klaphake by Don Paseka ...151

Schramm Park Fall Field Days, Sept. 21-23, 2022 ... 154

Index to Volume 90 ...159

Subscription and Organization Information …175

Remembering Clem Klaphake, Don Paseka

Nebraska Bird Review

Clement (Clem) Klaphake passed away on December 30, 2022, from complications of Parkinson’s disease, which he struggled with for several years. He was born in Melrose, Minnesota, in 1943 and grew up working on his parents’ dairy farm, which included the usual menagerie of other farm animals typical of that time. Perhaps it was the routine of milking twice a day, seven days a week, or the impressive scar on his leg from a boar tusk, but Clem was destined to leave the farm and become a life-long educator. ... After getting his degree in 1971, Clem was hired to …

Urban Wildlife Management Planning Process And Conflict Mitigation: A Case Study Of Denver’S Canada Goose Management Plan, Brent Johannes

Community and Regional Planning Program: Theses

In 2019 and 2020, the USDA in coordination with Denver Parks and Recreation removed 2,174 geese from 6 different parks within the City of Denver. The removals and use of lethal methods to manage the concerns related to the geese population in Denver’s parks caused a public conflict and resulted in multiple legal challenges with the City of Denver. The opposition group claimed that the city did not sufficiently engage with the public in the formation of the goose management plan, and did not provide any public notification about the plan to remove geese. City officials have claimed that attempts …

Timing Of Implants Use In Backgrounding System, Kylie M. Butterfield

Applied Science Program: Theses

A study was conducted over two years to determine the interactions of winter rate of gain at a high gain (HG) of 0.91 kg and low gain (LG) 0.45 kg, and implant strategy in the backgrounding and subsequent effects in the finishing phases. There were three phases in this study, the winter phase (~148 days), the summer phase (~60 days) and the finishing phase (~120 days). This was a 2 x 3 factorial design. The first factor is a rate of gain at either 0.91 kg (HG) or 0.45 kg (LG). The next factor is the timing of implant where …

Bevers: A General, Simple, And Performant Framework For Automatic Fact Verification, Mitchell Dehaven

School of Computing: Dissertations, Theses, and Student Research

Fact verification has become an important process, primarily done manually by humans, to verify the authenticity of claims and statements made online. Increasingly, social media companies have utilized human effort to debunk false claims on their platforms, opting to either tag the content as misleading or false, or removing it entirely to combat misinformation on their sites. In tandem, the field of automatic fact verification has become a subject of focus among the natural language processing (NLP) community, spawning new datasets and research. The most popular dataset is the Fact Extraction and VERification (FEVER) dataset. In this thesis an end-to-end …

Eco-Dystopian Novels Written By Women: Second, Third, And Fourth Wave Feminism, Trystyn Cox

Department of Environmental Studies: Undergraduate Student Theses

The project is thematic; analysis of eco-dystopian novels written by American female authors in the 20th and 21st centuries in order to determine if the different waves of feminism experienced in the United States had any influence over the books. By doing an analysis of eco-dystopian novels the study identifies if there were any outside societal movements or issues going on at the time that may have influenced how the book was created or if a particular book was able to open innovative ideas to the society at the time it was published. I researched the second, third, and fourth …

Food Security & Urban Agriculture - Considerations For Soil Improvements And Crop Planning, Aaron Sedivy

Doctor of Plant Health Program: Dissertations and Student Research

Urban agriculture provides many benefits, such as energy conservation, rainwater remediation, increased aesthetic and property value, and therapeutic use for physical, cognitive, and emotional wellbeing. Urban agriculture can also provide critical integrity to each pillar of food security: availability, access, utilization, and stability. As urban agricultural activities increase, social interactions will follow in concert. This will provide opportunities for building trust through the two-way communication of contrasting perspectives, cultures, and beliefs using impartial narratives. Growers can use knowledge and understanding gained from these positive social interactions to formulate their personal paradigms for practice and better meet the needs of each …

The North Platte River Valley: The Intersectionality Between Water Quality And People, Anni Poetzl

School of Natural Resources: Dissertations, Theses, and Student Research

The North Platte River (NPR) Valley of western Nebraska is a semi-arid watershed with row crop production, livestock production, and urban land use activity and has a population of diverse stakeholders. These land use activities contribute to the enrichment of surface waters, such as streams, which can affect human and ecosystem health, as well as economic development and recreational activities. The project objectives are to: (1) quantify the movement of dissolved inorganic nutrients from the land within the NPR Valley to the NPR via tributaries and canals, (2) identify spatiotemporal variability of nutrient limitation of periphyton growth within the NPR, …

Human And Hydrologic Influences On Nebraska's Endangered Rainwater Basin Wetlands, Sarah Thompson

Department of Earth and Atmospheric Sciences: Dissertations, Theses, and Student Research

Over half of wetlands in North America have been lost or degraded. Rainwater Basin (RWB) wetlands, located in south-central Nebraska, are a primary example of such loss; an estimated 90% have been destroyed by land conversion for agriculture. Remaining RWB wetlands are often embedded in row-crop fields, where they are threatened by altered surface water runoff flow, drainage features, and excess sediment inputs. Efforts at the state and federal level have been made to preserve this wetland complex due to the critical stopover habitat these wetlands provide for migratory birds. Land managers work to maintain sufficient water levels during migratory …

Sequence-Based Bioinformatics Approaches To Predict Virus–Host Relationships In Archaea And Eukaryotes, Yingshan Li

School of Computing: Dissertations, Theses, and Student Research

Viral metagenomics is independent of lab culturing and capable of investigating viromes of virtually any given environmental niches. While numerous sequences of viral genomes have been assembled from metagenomic studies over the past years, the natural hosts for the majority of these viral contigs have not been determined. Different computational approaches have been developed to predict hosts of bacteria phages. Nevertheless, little progress has been made in the virus-host prediction, especially for viruses that infect eukaryotes and archaea. In this study, by analyzing all documented viruses with known eukaryotic and archaeal hosts, we assessed the predictive power of four computational …