Digital Commons Network^™

Developing Guidelines For The Use Of Lightweight Materials In Culvert Preservation, Charlie Sun, Kean H. Ashurst Jr.

Kentucky Transportation Center Research Report

This study addresses challenges that arise during highway embankment construction on road widening projects when additional fill is placed above existing culverts. Researchers conducted reduced-scale model laboratory tests to simulate culvert behavior under different loading conditions and to determine how well lightweight materials (LWMs) perform when subjected to changing loads. Testing showed that proximity of LWM to the culvert’s top surface strongly influences the magnitude of strain reductions or increases. Placing LWMs with relatively low elastic modulus closer to the culvert’s top surface led to reduced culvert ceiling strain and increased culvert wall strain. LWMs with relatively high elastic modulus …

Addressing Multimodal Transportation Needs Through Complete Streets Implementation, Eirini Stavropoulou, Nikiforos Stamatiadis, William Staats, Teng Wang, Reginald Souleyrette

Kentucky Transportation Center Research Report

Since the early 2000s, over 1,600 transportation agencies in the United States have adopted Complete Streets policies. Recently, the Kentucky Transportation Cabinet (KYTC) published its Complete Streets, Roads, and Highways Manual, which aims to implement a safe and equitable transportation system throughout the state, as well as a Complete Streets policy. The manual and policy offer guidance on integrating Complete Streets principles into road design, however, KYTC currently lacks tools or methods to evaluate how well specific projects address Complete Streets goals. This is problematic because systematic assessments are needed to effectively prioritize projects and allocate scarce funding. Based …

Estimation Of Benefits From Pedestrian And Bicycle Improvements, Dionysios Tzamakos, Nikiforos Stamatiadis, William Staats, Teng Wang, Reginald Souleyrette

Kentucky Transportation Center Research Report

Delivering safe mobility for all users of the transportation system is a key priority of the Kentucky Transportation Cabinet (KYTC). Because many system users rely on pedestrian and bicycle facilities, it is critical for KYTC to balance pedestrian and bicycle facility accommodations with those for motorized users. Currently, the Strategic Highway Investment Formula for Tomorrow (SHIFT) — the Cabinet’s systematic approach to scoring and prioritizing proposed roadway projects — awards up to five points (out of a possible total of 100) for pedestrian and bicycle projects. But the agency lacks a robust method for estimating the benefits of pedestrian and …

Rees Algebras Of Unit Interval Determinantal Facet Ideals, Ayah Almousa, Kuei Nuan Lin, Whitney Liske

Mathematics Faculty Publications

Using SAGBI basis techniques, we find Gröbner bases for the presentation ideals of the Rees algebras and special fiber rings of unit interval determinantal facet ideals. In particular, we show that unit interval determinantal facet ideals are of fiber type and that their special fiber rings are Koszul. Moreover, their Rees algebras and special fiber rings are normal Cohen-Macaulay domains and have rational singularities.

Balancing Environmental Clearances With Construction Work Zone Needs, Chris Van Dyke, Steve Waddle, Ying Li

Kentucky Transportation Center Research Report

On small, single-span bridge projects (< 100 feet), the Kentucky Transportation Cabinet (KYTC) during project development works to establish an environmentally clear area (ECA) large enough to accommodate contractor operations. An adequately sized ECA gives contractors sufficient room to have materials delivered, park vehicles, store materials, and maneuver equipment around the project site (e.g., cranes, drill rig). If a contractor finds an ECA is too small to support their operations, they are responsible for obtaining additional environmental clearances. Clearing additional areas following the project award can slow down project delivery. To reduce the likelihood of contractors needing to enlarge an ECA, this report includes four documents KYTC can implement immediately to streamline and clarify the environmental clearances process. They include a checklist that project managers can use to coordinate the work of project team members and verify environmental clearances are on track, a combined environmental clearances workflow and best practices document for use by environmental subject-matter experts (SMEs), proposed language that can be inserted into project proposals that describes the environmental clearance process and specifies materials the Cabinet will hand off to contractors showing the location of the ECA, and a one-page explainer for contractors describing the environmental clearances process. Taken together, these deliverables emphasize the importance of early and ongoing coordination among project team members, clearing as much area as practical in and around bridges, and being transparent with contractors about their responsibilities.

Are “10 Grams Of Protein” Better Than “Ten Grams Of Protein”? How Digits Versus Number Words Influence Consumer Judgments, Marisabel Romero, Adam W. Craig, Milica Mormann, Anand Kumar

Marketing & Supply Chain Faculty Publications

Numerical information can be communicated using different number formats, such as digits (“5”) or number words (“five”). For example, a battery product may claim to last for “5 hours” or “five hours.” And while these two formats are used interchangeably in the marketplace, it is not clear how they influence consumer judgments and behavior. Via six experimental studies, two online ad campaigns, and one large secondary dataset analysis, we find that digits, compared to number words, positively affect consumer behavior. We refer to this phenomenon as the number format effect. We further show that the number format effect occurs because …

Forage News [2024-05], University Of Kentucky

Forage News

No abstract provided.

Matching Study Design To Prescribing Intention, Bethany L. Diprete, Gyeon Oh, Daniela C. Moga, Nabarun Dasgupta, Svetla Slavova, Emily Slade, Chris Delcher, Brian W. Pence, Shabbar I. Ranapurwala

Biostatistics Faculty Publications

Purpose: In drug studies, research designs requiring no prior exposure to certain drug classes may restrict important populations. Since abuse-deterrent formulations (ADF) of opioids are routinely prescribed after other opioids, choice of study design, identification of appropriate comparators, and addressing confounding by “indication” are important considerations in ADF post-marketing studies. Methods: In a retrospective cohort study using claims data (2006–2018) from a North Carolina private insurer [NC claims] and Merative MarketScan [MarketScan], we identified patients (18–64 years old) initiating ADF or non-ADF extended-release/long-acting (ER/LA) opioids. We compared patient characteristics and described opioid treatment history between treatment groups, classifying patients as …

Quantifying Image Quality In Aoslo Images Of Photoreceptors, Brea D. Brennan, Heather Heitkotter, Joseph Carroll, Sergey Tarima, Robert F. Cooper

Biostatistics Faculty Publications

The use of “quality” to describe the usefulness of an image is ubiquitous but is often subject to domain specific constraints. Despite its continued use as an imaging modality, adaptive optics scanning light ophthalmoscopy (AOSLO) lacks a dedicated metric for quantifying the quality of an image of photoreceptors. Here, we present an approach to evaluating image quality that extracts an estimate of the signal to noise ratio. We evaluated its performance in 528 images of photoreceptors from two AOSLOs, two modalities, and healthy or diseased retinas. The algorithm was compared to expert graders’ ratings of the images and previously published …

Identification Of Rnai-Related Genes And Transcriptome Assembly Of Loblolly Pine (Pinus Taeda, L.) Seedlings Exposed To Insect-Specific Dsrna, Zachary Bragg, Lynne K. Rieske

Entomology Faculty Publications

Considerable research has focused on gene silencing in tree-feeding insects, but how trees recognize and process double-stranded RNA (dsRNA) engineered to target plant pests is unknown. We performed transcriptomic assembly, preliminary differential expression analysis, and in silico annotation on loblolly pine (Pinus taeda, L.) seedlings exposed to southern pine beetle-specific dsRNA. This pilot study sought to elucidate the baseline response of seedlings challenged with insect-specific dsRNA. Treated and untreated seedlings were sequenced and following transcriptome assembly 20 RNAi-related proteins (RRPs) were annotated. Differential gene expression analysis conducted using DESeq2 followed by pathway enrichment revealed 7131 differentially expressed transcripts, of which …

Effects Of Ferrite And Graphite Phases On Scratch Characteristics Of Nodular Cast Iron, Zhitong Xu, Ming Liu, Chenghui Gao, Fuqian Yang

Chemical and Materials Engineering Faculty Publications

In this work, we conduct micro-scratch tests of nodular cast iron with Vickers indenter under constant normal load and investigate the effects of individual phases (i.e., ferrite and graphite phases) on the scratch charac- teristics of nodular cast iron. Using a bimodal Gaussian model to fit the experimental data of penetration depth, residual depth, elastic recovery rate, and scratch hardness, we calculate the contribution percentages of indi- vidual phases to scratch variables. The scratch variables are independent of the scratch speed used in this work, while the penetration depth increases linearly with increasing the normal load. There exists a large …

A Mechanical Model For Stress Relaxation Of Polylactic Acid/Thermoplastic Polyurethane Blends, Yi-Sheng Jhao, Hao Ouyang, Chien-Chao Huang, Fuqian Yang, Sanboh Lee

Chemical and Materials Engineering Faculty Publications

Polylactic acid (PLA) is considered a promising biodegradable polymer alternative. Due to its high brittleness, composite materials made by melt blending thermoplastic polyurethane (TPU) with PLA can enhance the toughness of PLA. To understand the forced aging caused by stress relaxation in polymer materials, this study explains the stress relaxation experiments of PLA/TPU blends with different mass ratios under applied strain through mechanical model simulations. The Kelvin representation of the standard linear solid model (SLSM) is used to analyze the stress relaxation data of TPU/PLA blends, successfully explaining that the Young’s moduli (E1 and E2) of springs decrease with increasing …

Plant Model Of Α-Synucleinopathy: Expression Of Α-Synuclein A53t Variant In Hairy Root Cultures Leads To Proteostatic Stress And Dysregulation Of Iron Metabolism, Jasmina Kurepa, Kristen A. Bruce, Greg A. Gerhardt, Jan A. Smalle

Neurology Faculty Publications

Synucleinopathies, typified by Parkinson’s disease (PD), entail the accumulation of α- synuclein (αSyn) aggregates in nerve cells. Various αSyn mutants, including the αSyn A53T variant linked to early-onset PD, increase the propensity for αSyn aggregate formation. In addition to disrupting protein homeostasis and inducing proteostatic stress, the aggregation of αSyn in PD is associated with an imbalance in iron metabolism, which increases the generation of reactive oxygen species and causes oxidative stress. This study explored the impact of αSyn A53T expression in transgenic hairy roots of four medicinal plants (Lobelia cardinalis, Artemisia annua, Salvia miltiorrhiza, and Polygonum multiflorum). In all …

Regulation Of Rorα Stability Through Prmt5-Dependent Symmetric Dimethylation, Gaofeng Xiong, Brynne Obringer, Austen Jones, Elise Horton, Ren Xu

Markey Cancer Center Faculty Publications

Retinoic acid receptor-related orphan receptor alpha (RORα), a candidate tumor suppressor, is prevalently downregulated or lost in malignant breast cancer cells. However, the mechanisms of how RORα expression is regulated in breast epithelial cells remain incompletely understood. Protein arginine N-methyltransferase 5 (PRMT5), a type II methyltransferase catalyzing the symmetric methylation of the amino acid arginine in target proteins, was reported to regulate protein stability. To study whether and how PRMT5 regulates RORα, we examined the direct interaction between RORα and PRMT5 by immunoprecipitation and GST pull-down assays. The results showed that PRMT5 directly bound to RORα, and PRMT5 mainly symmetrically …

A Cautionary Tale About Properly Vetting Datasets Used In Supervised Learning Predicting Metabolic Pathway Involvement, Erik D. Huckvale, Hunter N. B. Moseley

Markey Cancer Center Faculty Publications

The mapping of metabolite-specific data to pathways within cellular metabolism is a major data analysis step needed for biochemical interpretation. A variety of machine learning approaches, particularly deep learning approaches, have been used to predict these metabolite-to-pathway mappings, utilizing a training dataset of known metabolite-to-pathway mappings. A few such training datasets have been derived from the Kyoto Encyclopedia of Genes and Genomes (KEGG). However, several prior published machine learning approaches utilized an erroneous KEGG-derived training dataset that used SMILES molecular representations strings (KEGG-SMILES dataset) and contained a sizable proportion (~26%) duplicate entries. The presence of so many duplicates taint the …

Structural Changes Of Flaxseed Protein Modified By Fermentation And The Gel Properties And Swallowing Characteristics Of Its Composite System With Mung Bean Starch, Cong Min, Jingzhang Geng, Chengxin Liu, Yuhan Wang, Junrong Huang, Youling L. Xiong

Animal and Food Sciences Faculty Publications

The fermentation modification of plant-based proteins has attracted extensive attention recently. This study was to investigate the effect of fermentation on molecular structures of flaxseed protein (FP), and evaluate the gel properties and swallowing characteristics of the composite gel composed of mung bean starch (MBS) and fermented FP (FFP). After 10 h of fermentation, the content of free amino acids in FFP increased ∼14.3 times over that of the unfermented FP, while the proportion of small peptides increased by ∼37.2%. Fermentation reduced the α-helical conformation in FFP. After heating, FFP was easier to cross-link with MBS to form strengthened composite …

Ileal Phosphorus Digestibility Of Soybean Meal For Broiler Chickens Remains Consistent Across Institutions In A Collaborative Study Regardless Of Non-Phytate Phosphorus Concentration In The Pre-Experimental Starter Diet, V. S. Haetinger, J. Y. Sung, S. A. Adedokun, W. A. Dozier, C. M. Parsons, M. Rodehutscord, O. Adeola

Animal and Food Sciences Faculty Publications

The same experimental protocol was used in 4 institutions to evaluate the impact of non-phytate phosphorus (nPP) concentration in the starter diet on regression method-derived ileal P digestibility of soybean meal (SBM) during the subsequent grower phase. A total of 1,536 Ross 308 male broiler chickens on d 0 post hatching were allotted to 2 pre-experimental starter diets that contained 3.5 or 4.5 g nPP/kg (96 replicate cages per diet, 8 birds per cage) for 18 d. Subsequently, 576 birds from each starter diet were selected and allocated to 3 experimental semi-purified grower diets containing 400, 510, or 620 g …

Effect Of Simulated Gastrointestinal Digestion On Antioxidant, And Anti-Inflammatory Activities Of Bioactive Peptides Generated In Sausages Fermented With Staphylococcus Simulans Qb7, Hongying Li, Hongbing Fan, Zihan Wang, Qiujin Zhu, Jianping Wu

Animal and Food Sciences Faculty Publications

Dry-fermented sausages are a good source of bioactive peptides, whose stability against gastrointestinal (GI) digestion determines their bioaccessibility. This study focused on evaluating the effect of peptide extracts from sausages fermented with Staphylococcus simulans QB7 during in vitro simulated GI digestion, including peptide profi les and antioxidant and anti-infl ammatory activities. Peptides present in sausages were degraded during digestion, with molecular weight reduced from > 12 kDa to < 1.5 kDa. Besides, the content of amino acids increased from 381.15 to 527.07 mg/g, especially tyrosine being found only after GI digestion. The anti-infl ammatory activities were increased after GI digestion, however, the changes in antioxidant activities were the opposite. A total number of 255, 252 and 386 peptide sequences were identified in undigested, peptic-digested and GI-digested samples, respectively. PeptideRanker, BIOPEP-UWM and admetSAR were used to further predict the functional properties and intestinal absorption of the identified peptide sequences from GI digestion. Finally, 18 peptides were discovered to possess either antioxidant or anti-infl ammatory capacities.

Southern Region Produce Safety Alliance Grower Training, Keith R. Schneider, Mari Schroeder, Alan Gutierrez, Karuna Kharel, Renée Goodrich Schneider, Amy Harder, Amanda Philyaw Perez, Kristin Woods, Laurel L. Dunn, Paul Priyesh, Christopher Gunter, Elena Rogers, Chip Simmons, Lynette Johnston, Chad Carter, Thomas M. Taylor, Alejandro Castillo, Juan Anciso, Joseph Masabni, Laura K. Strawn, Amber Vallotton, Katelynn Stull, Taylor O'Bannon, Michelle D. Danyluk

Animal and Food Sciences Faculty Publications

The Produce Safety Alliance (PSA) grower training was introduced in 2016 as the standardized curriculum to meet the training requirements of the Food and Drug Administration's (FDA) Food Safety Modernization Act's (FSMA) Produce Safety Rule (PSR). The PSR states that at least one supervisor or responsible party from each farm must have successfully completed this food safety training or one equivalent to the standardized curriculum, as recognized by the FDA. This study evaluated the effectiveness of PSA trainings conducted between 2017 and 2019 in the Southern United States by the Southern Regional Center for Food Safety Training, Outreach, and Technical …

Ensuring Equitable Application Of Interventions To Vulnerable Subpopulations In The Kentucky Consortium For Accountable Health Communities (Kc-Ahc), Jing Li, Jessica M. Clouser, Akosua Adu, Aiko Weverka, Nikita Vundi, Terry D. Stratton, Mark V. Williams

Journal of Appalachian Health

Introduction: The Centers for Medicare and Medicaid Services (CMS) has funded the Accountable Health Communities (AHC) model to test whether systematically identifying and addressing the health-related social needs (HRSNs) of individuals would impact healthcare utilization and total cost of care for Medicare and Medicaid beneficiaries. Toward this effort, AHCs implement screening, referral, and community navigation services in their local areas. There are 28 CMS-funded AHCs nationwide, including the Kentucky Consortium for Accountable Health Communities (KC-AHC).

Purpsoe: This study aims to assess the equity of KC-AHC model activities in three vulnerable sub-populations: dual enrollees, disabled individuals, and women.

Methods: Twenty-eight primary …

Maternal Age And Inadequate Prenatal Care In West Virginia: A Project Watch Study, Madelin Gardner, Amna Umer, Brian Hendricks, Toni Marie Rudisill, Candice Lefeber, Collin John, Christa Lilly

Journal of Appalachian Health

Introduction: Adequate prenatal care (PNC) is essential to the overall health of mother and infant. Teen age and advanced maternal age (AMA) are known risk factors for poor birth outcomes. However, less is known about whether these age groups are associated with inadequate PNC.

Purpose: This study sought to determine the potential association between maternal age (in groups, aged 20–24, 25–29, 30–34, 35–39, and >40) and inadequate PNC (visits).

Methods: West Virginia (WV) Project WATCH population-level data (May 2018–March 2022) were used for this study. Multiple logistic regressions were performed on inadequate PNC (less than 10 visits) with maternal age …

Trilogies: Lessons From 50 Years Facilitating Community-Based Health Assessments And Planning In Appalachia, Bruce Behringer

Journal of Appalachian Health

Involvement of community and organizational groups is fundamental to most public ventures. Most social, health, economic, and educational improvements in Appalachia have been characterized by successfully integrating community input and finding ways to encourage organizational change and collaboration.

Managing group process and related facilitation skills are fundamental competencies for public health professionals and others guiding change efforts. Groups from communities and organizations can get stalled in their deliberations; a facilitator frequently must think quickly to diagnose the situation and propose alternative approaches. Creative and flexible approaches, learned through practice experiences, can blend with theories and frameworks learned in academic preparation …

Overexpression Of Fatty Acid Synthase Upregulates Glutamine–Fructose-6-Phosphate Transaminase 1 And O-Linked N-Acetylglucosamine Transferase To Increase O-Glcnac Protein Glycosylation And Promote Colorectal Cancer Growth, James Drury, Mariah E. Geisen, Josiane Weber Tessmann, Piotr G. Rychahou, Courtney O. Kelson

Markey Cancer Center Faculty Publications

Fatty acid synthesis has been extensively investigated as a therapeutic target in cancers, including colorectal cancer (CRC). Fatty acid synthase (FASN), a key enzyme of de novo lipid synthesis, is significantly upregulated in CRC, and therapeutic approaches of targeting this enzyme are currently being tested in multiple clinical trials. However, the mechanisms behind the pro- oncogenic action of FASN are still not completely understood. Here, for the first time, we show that overexpression of FASN increases the expression of glutamine–fructose-6-phosphate transaminase 1 (GFPT1) and O-linked N-acetylglucosamine transferase (OGT), enzymes involved in hexosamine metabolism, and the level of O-GlcNAcylation in vitro …

Treatment At An Academic Medical Center Eliminates Survival Disparities For Appalachian Kentuckians With Pancreatic Ductal Adenocarcinoma, Emily Cassim, Hannah Mcdonald, Megan Harper, Quan Chen, Miranda Lin, Reema Patel, Michael Cavnar, Prakash Pandalai, Bin Huang, Pamela C. Hull, Joseph Kim, Erin Burke

Journal of Appalachian Health

Introduction: Rates of cancer mortality in Appalachian Kentucky is among the highest in the nation. It is unknown whether geographic location of treatment for pancreatic ductal adenocarcinoma (PDAC), one of the deadliest cancers worldwide, influences survival in Appalachian Kentuckians.

Purpose: This study compares outcomes among Appalachian Kentuckians with PDAC who received treatment at an academic medical center (AMC) or community facility (CF).

Methods: Using the Kentucky Cancer Registry, patients diagnosed with PDAC between 2003 and 2018 were identified. Patients were categorized according to treatment location (AMC v. CF) and county of residence (Appalachian v. non-Appalachian). Kaplan-Meier curves were constructed to …

Conscientious Objection: Understanding When And Why Primary Care Physicians Object To Providing Health Care To Transgender And Gender-Diverse Patients In An Appalachian Medical Center, Mili S. Patel, Kelley A. Jones, Laura Davisson, Elizabeth Miller, Nicole Kahn, Pamela J. Murray, Kacie M. Kidd

Journal of Appalachian Health

Introduction: Transgender and gender-diverse (TGD) individuals face barriers to accessing primary and gender-affirming care, especially in rural regions where a national shortage of medical providers with skills in caring for TGD people is further magnified. This care may also be impacted by individual providers’ strongly held personal or faith beliefs and associated conscientious objection to care.

Purpose: This study assesses the prevalence of conscientious objection to providing care and gender-affirming hormone (GAH) therapy to TGD individuals among physicians in an Appalachian academic medical center.

Methods: An anonymous, online, cross-sectional survey of physicians was distributed to resident and faculty physicians in …

Check With The Intended Audience First! Content Validation As A Method For Inclusive Research For Primary Care Engagement In Rural Appalachia, Sydeena E. Isaacs, Jennifer Schroeder Tyson, Ashley Parks, Danielle Adams

Journal of Appalachian Health

Introduction: To date, referral practices based on social determinants of health (SDOH) among primary care providers (PCPs) and clinic staff in rural regions, including Appalachian North Carolina (NC), are not well understood.

Purpose: This study aims to develop and content validate a primary care engagement (PCE) survey to assess (1) engagement and burnout; (2) referral practices; and (3) self-efficacy and confidence in making referrals based on SDOH among PCPs and clinic staff in Appalachian NC.

Methods: Using the Social-Ecological Model as a theoretical framework, researchers developed a 37-item PCE survey. Content validation was completed by a panel of experts recruited …

Building A Roadmap For Web Archiving: Organizational Sustainability In An American Research University Library, Ruth E. Bryan, Emily B. Collier

Library Presentations

The presenters, archivists in an academic university Library, launched a web archiving program for a public university in the United States in 2018 with a three-year Archive-it contract. In the first six years of the web archiving program, we have laid the groundwork for an ongoing web archiving program through robust documentation built in anticipation of potential loss of resources, especially personnel. In this presentation, we report on a sustainability review of the program using a practical framework and a conceptual framework. The practical framework is the University of Pittsburg's Socio-Technical Sustainability Roadmap (https://sites.haa.pitt.edu/sustainabilityroadmap/). The conceptual framework is Kristin R. …

Preserving The Uncrawlable: Serving The Server, Andrew Mcdonnell

Library Presentations

Many of the challenges inherent in archiving dynamic web content revolve around the capture and playback of ever-evolving social media sites. However, older dynamic web content that has ceased to evolve continues to elude the tools and conventions most widely available to web archivists. This presentation will share work conducted to preserve an online digital humanities project that defied preservation via Archive-It and other crawler-based web archiving tools. The presentation will, moreover, offer an exploration of alternative options for web archivists struggling to preserve sites dependent on server-side processes for their essential functionality.

Suda On Line is a collaborative digital …

Decistifying Trans And Gender Diverse Inclusion In Library Work: A Literature Review, Stephen G. Krueger, Keahi Adolpho

Library Faculty and Staff Publications

This comprehensive review illuminates the current state of scholarly literature on trans and gender diverse inclusion in libraries, with the intention to provide a foundation and identify gaps for further research. Covering 50 works published between 2002 and 2023 in the areas of general inclusion, public libraries, academic libraries, experiences of library workers, archives, information behavior, and cataloging, we found that, with the exception of works on archives and cataloging, little scholarly literature goes beyond introductory talking points on basic information about trans and gender diverse people. We conclude with a call for much more in-depth research on this essential …

Influence Of Low-Level Condensed Tannins Concentrations In Temperate Forages On Sheep Performace, F Montossi, F Liu, J Hodgson, S T. Morris, T N. Barry, D F. Risso

IGC Proceedings (1977-2023)

Results from a series of six comparative lamb grazing trials with temperate forages are summarised, and compared with results from the literature. Condensed tannin (CT) concentrations in the diet ranged from 0.12% to 0.47% on a dry matter (DM) basis. Values were generally similar for Yorkshire fog (Holcus lanatus), perennial ryegrass (Lolium perenne) and annual ryegrass (L. multiflorum), each grown with white clover (Trifolium repens), in similar circumstances. Concentrations for tall fescue (Festuca arundinacea)/white clover pastures were relatively low, but may have been artefacts. Drenching with polyethylene glycol (PEG) to bind …