Digital Commons Network^™

Holding Space For Two Truths: Thematic Analysis Of Speech Language Pathologists’ And Teachers’ Philosophies And Practices In Reading Comprehension, Ryan Foote

Theses and Dissertations--Communication Sciences and Disorders

Introduction: Reading is a doorway to life participation. Only about one-third of fourth graders have read at grade level for the last three decades. Multiple views on what constitutes skilled reading emphasize decoding and greater variability among comprehension-related skills. Scarborough’s Reading Rope is the most specific in defining discrete skills necessary for adequate reading comprehension. This study aims to understand how elementary-level general education teachers and speech-language pathologists define “reading comprehension” and describe their practices in assessing and developing these skills. Methodology: Elementary school speech-language pathologists and teachers were interviewed about their conceptualizations and applications of the discrete skills in …

Practicing Clinicians' Views Of The Life Participation Approach To Aphasia Therapy, Stephanie H. Mays

Theses and Dissertations--Communication Sciences and Disorders

The Life Participation Approach to Aphasia therapy (LPAA) aligns with a person-centered, functional approach to speech therapy. Past research has demonstrated that persons with aphasia (PWA) benefit from this approach and prefer it when compared to the traditional medical model to therapy. However, a disconnect between PWA preferences and SLP treatment approaches in therapy persists. This study aims to identify both barriers and facilitators that SLPs face when implementing LPAA. Five CCC-SLPs practicing in the state of KY with experience working with PWA were recruited as participants. Participants were placed into two focus groups, with each group meeting for 2-3 …

M1 Macrophage-Engineered Vesicles Have Anti-Cancer Activity In Ovarian Cancer, Connie Du Cao

Theses and Dissertations--Clinical and Translational Science

Ovarian cancer typically presents at an advanced stage, has a poor prognosis, and is a leading cause of cancer-related deaths in women. Extracellular vesicles (EVs) are cell-derived membrane-bound nanoparticles that function in specific cell-to-cell communication and are under development as novel drug delivery vehicles and modulators of the tumor microenvironment. Artificial cell-derived vesicles (ACDVs) derived from M1 macrophages are able to repolarize M2 macrophages to the M1 phenotype and target tumor cells in in vitro studies.

In this study, we generated engineered EVs (EEVs) by membrane disruption of M1 macrophages (MEVs) in the presence and absence of cisplatin to generate …

Zebrafish Models To Uncover Targetable Mechanisms Of Self-Renewal In T-Cell Acute Lymphoblastic Leukemia, Majd Al-Hamaly

Theses and Dissertations--Pharmacology and Nutritional Sciences

Relapsed T-cell Acute Lymphoblastic Leukemia (T-ALL) patients have dismal prognosis, with 5-year overall survival rates of 10% for adults and 36% for children. Relapse is attributed to the inability of chemotherapy agents to eliminate Leukemia Stem Cells (LSCs) that can repopulate the leukemia. Those cells exist with a unique genetic, transcriptomic, and metabolic profile compared to the rest of the leukemia and thus, finding targeted therapies toward this population of cells can result in disease eradication and elimination of relapse. However, investigating LSCs is challenging-particularly in T-ALL- for two reasons: 1) the lack of robust surface markers that define LSCs …

Cell-Type Specific Apoe4 To Apoe2 ‘Switching’ In Astrocytes And Microglia Alters Alzheimer’S Disease Neuropathology, Lesley R. Golden

Theses and Dissertations--Physiology

Apolipoprotein E (APOE) is the strongest genetic risk factor for late-onset Alzheimer’s disease (LOAD). APOE exists in three common protein isoforms throughout the population: ApoE2 (E2), ApoE3 (E3), and ApoE4 (E4). While APOE allele frequencies and the degree of AD-associated risk vary across different ethnic groups, when compared to the ‘neutral’ and most common E3 allele, the E4 allele confers up to a 33-fold increase in Alzheimer’s Disease (AD) risk. Conversely, the neuroprotective E2 allele decreases AD risk by up to 67%. Here, we aimed to determine the therapeutic potential of cell-type specific APOE allele ‘switching’ and explore …

Biochemical And Biophysical Properties Of Failing Myocardium In Rare Patient Populations, Gregory Nathaniel Milburn

Theses and Dissertations--Physiology

Heart failure has diverse underlying etiologies, complicating research. While some types, like ischemic disease or inherited mutations, are well-studied, research on rarer forms has lagged. Understanding the molecular drivers in these populations may enhance diagnostics and therapies. This work examines two rare groups: patients with ATTR cardiac amyloidosis and those receiving mechanical assist device support. We show that myocardium from patients with amyloidosis has increased fibrosis and reduced force production without changes in cross-bridge kinetics. Additionally, we show that the passive stiffness of amyloidosis myocardium is not different from non-failing myocardium, indicating increased ventricular wall thickness may cause restrictive filling. …

Biochemical Properties Of Cardiac Sarcomeres In Human Heart Failure, Austin Grey Wellette-Hunsucker

Theses and Dissertations--Physiology

Heart failure (HF) exhibits considerable heterogeneity, posing significant challenges for research involving human cohorts. Diastolic dysfunction is a common pathophysiological feature; however, its molecular mechanisms remain incompletely elucidated. Sarcomeric proteins are integral to cardiac contraction and relaxation. Nonetheless, the phosphoproteome and the structural remodeling effects of these proteins on diastolic function within the context of HF have not been comprehensively characterized. This study examined sarcomeric modifications in diastolic dysfunction in human HF, focusing on Dilated and Ischemic HF, with non-failing donor hearts as controls. Analyzing myocardial samples from ~150 patients using electrophoretic and biochemical techniques, it found significant titin remodeling …

Development And Clinical Validation Of Automated Same-Day Stereotactic Treatment Procedures For Multiple Brain Metastases And Recurrent Head And Neck Cancers, Shane Mccarthy

Theses and Dissertations--Radiation Medicine

As technology advances, more and more cancer patients are benefiting from the use of stereotactic radiosurgery and radiotherapy (SRS/SRT) to treat their disease. One such technology, Varian’s HyperArc high-definition radiosurgery module, a tool to help facilitate the treatment of brain metastases on a medical linear accelerator (LINAC), has been demonstrated to achieve comparable low dose spillage and target conformity compared to the current gold standard for intracranial malignancies, the Elekta Gamma Knife. Furthermore, due to HyperArc’s single-isocenter multiple lesion approach, the treatment can be delivered in a significantly shorter amount of time. Unfortunately, the manual process of creating these stereotactic …

Assessing The Effectiveness Of A Modified Cook Together Eat Together Program On Food Resource Management Behaviors In A Rural Setting, Johanna K. Poysti

Theses and Dissertations--Nutrition and Food Systems

This study evaluated the effectiveness of a modified, hybrid Cook Together, Eat Together (CTET) program on food resource management behaviors in a rural setting. A six-week intervention combining virtual and in-person sessions aimed to improve health behaviors, food resource management skills, and self-efficacy. Although most behavioral changes were not statistically significant, participants reported increased confidence in cooking healthy meals on a budget. Positive qualitative feedback from focus group discussions indicated program feasibility and engagement. Strengths and limitations of the study were discussed as well as recommendations for future research were given.

Therapist Perspective Of Therapeutic Alliance And Treatment Outcome In Systemic Psychotherapy, Benjamin Larkin Mize Patterson

Theses and Dissertations--Family Sciences

One of the most robust findings in psychotherapy outcome research has been the relationship of a strong therapeutic alliance with improvement in treatment. Most research, however, has measured therapeutic alliance from the client’s point of view, disregarding the therapist’s perspective of this phenomenon. In this naturalistic study of adults in systemic individual, couple, or family psychotherapy (N = 627), both client- and therapist-ratings of alliance were assessed for their associations with treatment outcomes. Multilevel modeling was employed to analyze data from the Marriage and Family Therapy Practice Research Network (MFT-PRN). Client-ratings of the therapeutic alliance and general functioning/relationship satisfaction …

Causes And Consequences Of Reproductive Plasticity In The Seed Beetle, Callosobruchus Maculatus, Josiah P. Ritchey

Theses and Dissertations--Entomology

Competition for resources has direct effects on traits that can influence an individual’s reproductive success in competitive mating environments. When population density is high during development, competition for resources can reduce growth and body size at maturation, influencing resources available for reproduction. In this thesis, I investigate how the developmental environment affects plasticity in male reproductive traits, as well as the consequences of male reproductive plasticity on female reproductive behavior, in the seed beetle, Callosobruchus maculatus. My results show that increasing larval density causes male beetles to emerge small and transfer small ejaculates to females. However, small males transfer …

Dinotefuran Resistance In The German Cockroach (Blattella Germanica L.), Hannah F. Blevins

Theses and Dissertations--Entomology

German cockroaches (Blattella germanica L.) are one of the most prevalent and pervasive indoor pest species in the world. Infestations can lead to many negative impacts on humans, including sensitization to allergens, increase in asthmatic symptoms, mental health declines, and potentially, the spread of diseases. While insecticides remain the most effective and affordable option for German cockroach control, resistance to many commonly used insecticide active ingredients (AIs), such as pyrethroids, has been observed globally at high levels across different populations. Given such resistance to pyrethroids, inclusion of insecticides with alternative modes of action into insecticidal treatments could potentially improve cockroach …

Bed Bugs: Health Risks And Control, Simona Principato

Theses and Dissertations--Entomology

Bed bugs are blood-feeding hemipterans strongly associated with humans. Their detection in indoor environments, such as homes, hotels, trains, and public facilities, has increased gradually over the past 20 years. The exact cause of their resurgence is still unclear, but the increase of international travel and insecticide resistance are thought to have contributed to it.

Although bed bugs are not known to transmit diseases, the EPA, CDC, and USDA have classified them as a pest of significant public health concern, mainly due to the dermatitis that they can cause. Beyond the dermatological burden of the bites, other health implications often …

Family And Social Factors In Emotion Regulation And Adjustment In Adolescents, Julia J. Morgan

Theses and Dissertations--Family Sciences

Adolescents face many challenges during this unique phase of development. Because of this, it is important to identify how to promote resilience and positive adjustment for these individuals. The present dissertation includes three studies which focused on familial and social factors in promoting emotion regulation and ultimately adjustment among middle and late adolescents.

The first study focused on the importance of alexithymia in the relationships between closeness and communication and internalizing problems, namely depression, anxiety, and self-esteem. Results showed that closeness was negatively associated with depression and positively associated with self-esteem. Communication was not associated with any of the studied …

Childhood Chronic Illness: Assessing Emerging Adults’ Engagement In Social Activities And Predicted Money For Comfort In The Future, Mediated By Money Responsibility And Comparison To Others, Austin B. Mathews

Theses and Dissertations--Family Sciences

The purpose of this study is to examine the relationships between emerging adults’ engagement in social activities and their prediction of having money for comfort in the future. Data used was from the Panel Study of Income Dynamics (PSID) with individuals selected having at least one chronic illness that began during childhood. The study tested a series of hypothesis which tested the relationships between engagement in social activities and predicted money for comfort in the future, as well as, money responsibility and comparison to others as mediating variables. Significant associations were found between frequency of engagement in sports and money …

Debt Through The Decade: Exploring Connections Between Debt Type, Psychological Stress, Spending Habits, And Life Satisfaction, Emily Johnson

Theses and Dissertations--Family Sciences

Average households in the United States holds $101,915 in consumer debt, contributing to the greater total of America’s $17.3 trillion of total consumer debt (Experian, 2024). Accruing some amount of debt is considered normal throughout a financial lifespan, however, many Americans find themselves completely overwhelmed by keeping up with personal debts and increasing costs of necessities due to inflation. (George & Hansen, 2018; Kim et al., 2019). Following the financial crisis in 2020, many individuals felt increasing financial hardship, with many Americans heavily impacted by their debt holdings. The purpose of the present study is to examine the impact of …

The Role Of Nutrition In Managing Honey Bee Viral Pathogen, Caroline Wylie Kane

Theses and Dissertations--Entomology

This study evaluated the impact of nutritional supplements on viral infections in honey bee colonies, focusing on Lake Sinai Virus 2 (LSV2), Deformed Wing Virus A (DWVA), and Deformed Wing Virus B (DWVB). I partnered with 32 hobbyist beekeepers across Kentucky, who volunteered 138 colonies for a month-long field study. Half of the colonies received weekly commercial supplements, while the other half served as controls. Viral titers were measured before and after the supplementation period using qPCR. Supplements did not significantly reduce viral titers for any of the three viruses, suggesting that short-term supplementation may not be effective in lowering …

Exportin 1 Is The Central Hub In Antiviral Defensive Mobilization Of Nuclear Factors And The Target Of Counter-Defence By Tombusviruses, Biao Sun Dr.

Theses and Dissertations--Plant Pathology

Positive-strand RNA viruses, such as coronaviruses and flaviviruses, are obligate parasites whose genomic RNAs function as mRNAs. These viruses infect a wide range of hosts-including plants, animals, and humans-resulting in significant economic losses and posing serious threats to public health. Due to the relatively simple structure of the genome of positive-strand RNA viruses, many host factors and physiological processes are co-opted to support virus infection and replication. In our previous studies, we identified numerous host factors and associated physiological processes involved in the replication of tombusviruses, a cytosolic-replicating model that includes tomato bushy stunt virus (TBSV) and carnation Italian ringspot …

Role Of Chemotype In Aggressiveness, Toxicity, And Competitive-Ness Of Fusarium Graminearum On Wheat, Simran Goyal

Theses and Dissertations--Plant Pathology

Fusarium Head Blight (FHB) of wheat, caused by the fungus Fusarium graminearum, is a devastating disease that led to epidemics in the U.S. during the 1990s, resulting in economic losses exceeding $2.6 billion. Typical symptoms include bleached spikelets and shriveled grains, often contaminated with trichothecene mycotoxins that make them unsafe for food or feed. FHB severity and mycotoxin production are influenced by a complex interplay between host genotype, environmental factors, and pathogen genotype. Integrated management practices that include the use of more resistant cultivars, crop rotation, and fungicide applications help to reduce disease incidence and severity. Disease forecasting models …

Gene Expression In Relation To Bone Fractures In Throughbred Racehorses, Anna M. Rauber-Ramos

Theses and Dissertations--Veterinary Science

Evidence that fractures in Thoroughbred racehorses are outcomes of chronic bone microdamage has led to extensive research into identifying horses at increased risk of fracture. This includes the use of biomarkers and messenger RNA (mRNA) as a measure of bone turnover. Previous research has found expression differences in horses with fractures compared to controls. Utilizing pre-race whole blood samples collected over an 18-month period from Thoroughbred racehorses in Southern California, RNA-sequencing was conducted on horses with bone fractures and race-matched controls to identify differences in gene expression. Focusing on third metacarpal (MC3) and proximal sesamoid bone (PSB) fractures, differentially expressed …

Engineering Novel Biomimetic Models Of Equine Host-Microbe Interactions, Lynn M. Leedhanachoke

Theses and Dissertations--Veterinary Science

One major limitation to the study of equine disease is the lack of in vitro models that accurately reflect the dynamic physiology of the horse. Thus, there is a pressing need to develop in vitro systems that recapitulate diverse equine tissue and organ microenvironments. To overcome this deficiency, we developed multiple biomimetic equine organoid systems that can be employed for a variety of investigations aimed at resolving outstanding questions in infectious disease pathogenesis, therapeutic development and drug metabolism, host defense mechanisms, immune-modulated disease, and equine developmental biology. Our innovative platform incorporates novel equine intestinal enteroid and airway organoid models derived …

Avian Response To Legacy Coal Mine Reforestation In The Cumberland Plateau, Kentucky, Madoline J. Varias

Theses and Dissertations--Forestry and Natural Resources

Surface coal mining and reclamation processes alter the ecological characteristics of landscapes, which affects the presence and distribution of native wildlife. The Forestry Reclamation Approach (FRA) is a coal mine reclamation method that aims to establish native forests on surface mines and has been widely implemented across the Central Appalachian region. Avian response the FRA, in particular, is unknown in Kentucky and limited throughout Appalachia. Thus, the goal of this project was to assess the effects of the FRA on avian community occupancy, species richness, and abundance in the Cumberland Plateau of Kentucky. Point count surveys were conducted across three …

Predicting Patterns Of Species Presence In Eastern Kentucky Using Fine- And Broad-Scale Site-Species Relationships, Kyra L. Shrout

Theses and Dissertations--Forestry and Natural Resources

Understanding site-species relationships is integral to effective forest management, as landscape factors influence both the presence of mature trees and the regeneration of future trees. This study examined the influence of topographic, soil, and canopy variables on species presence at both fine (i.e., stand-level) and broad (i.e., forest-wide) scales within Robinson Forest, Kentucky. At the fine-scale, using vegetation data from permanent sampling plots, LiDAR-derived topographic data, and historical soils data, the relationships between the species of interest and the landscape variables within a single watershed were analyzed using conditional inference tree (CTree) models. Elevation and slope aspect were the most …

Does Participation In An Innovative Livestock Exhibition Program Foster The Development Of Grit In Youth Across Rural, Micro, And Metropolitan Communities?, Reba V. Prather

Theses and Dissertations--Community & Leadership Development

This research examined whether livestock exhibition could be used to foster grit in youth across rural, micropolitan, and metropolitan communities. More specifically, the researcher sought to evaluate the impact an innovative livestock exhibition program had on youth resilience. Through partnerships with 4-H clubs and agricultural organizations, the initiative encourages community-driven involvement, allowing youth to take ownership of their growth and development. The program utilized agricultural animal husbandry experiences as a mechanism for youth development, exploring the broader impact of livestock engagement on character-building. By assessing the outcomes of these experiences, the research highlights the trials and tribulations associated with fostering …

Mapping The Impact Of Leadership Training: A Ripple Effects Mapping Of The Kentucky Extension Homemaker Association’S First Leadership Academy, Kelly May

Theses and Dissertations--Community & Leadership Development

This research study evaluates the first Kentucky Extension Homemaker Association (KEHA) Leadership Academy using the participatory, qualitative evaluation tool Ripple Effects Mapping (REM). This study explored the impact and outcomes of the training, both intended and unintended.

REM is a participatory evaluation method that begins with appreciative inquiry, then continues with a group discussion where stories are “mapped” or illustrated in real time. Using the Theming and Rippling Method of REM, the research team coded the information on site with inductive theming and those themes were then shared and confirmed with the group. After the event, the mapped data was …

Researching Dementia Indicators Among Rural Populations, Justin S. Yount

Theses and Dissertations--Community & Leadership Development

The study explored potential dementia indicators and risk factors among older adults residing in rural areas who are actively engaged in farming operations. Data was collected in three components a questionnaire, an audiogram test, and a memory impairment screening. 104 people responded to the questionnaire, 133 participants took the audiogram test, and 94 participated in the memory impairment screening. The findings revealed that 80.9% of participants showed no sign of cognitive impairment while 19.1% showed signs of possible impairment. Participants reported moderate levels of physical and social activity, but common risk factors such as elevated BMI, diabetes, hearing loss, and …

Exploring Belongingness, Loneliness, And Distinctiveness On A College Of Agriculture Campus, Sophia Jaramillo-Vasconez

Theses and Dissertations--Community & Leadership Development

The purpose of this study was to explore how sense of belonging, loneliness, and distinctiveness affect students in the Martin-Gatton College of Agriculture, Food and Environment (M-G CAFE). By examining the intersection of student demographics and their experiences, this research aimed to provide a deeper understanding of the dynamics that shape students’ agricultural experiences at the postsecondary level. This study investigates the relationship between student characteristics and their reported levels of belonging and loneliness, and how these factors influence perceptions of their representation and distinctiveness within the M-G CAFE program. An explanatory sequential mixed methods design was employed, beginning with …

Influence Of Agrochemical Mixtures On Nitrogen Transformations In Floating Treatment Wetlands, Matthew Russell

Theses and Dissertations--Biosystems and Agricultural Engineering

Controlling losses of nitrate-N from agricultural fields to ground and surface water environments is one of the most pressing water resources problems today. Increasing levels of nitrate-N pollution in surface and groundwater is a critical threat to human and environmental health across the globe. To mediate nitrate-N losses, natural and constructed wetlands have been increasingly used as nutrient mitigation technologies and strategies. The use of wetlands as a treatment approach for nitrogen in runoff is a common practice in agroecosystems, however, nitrate-N is not the sole constituent present in agricultural runoff, and other biologically active contaminants have the potential to …

Nanopesticide Fate And Transport In Agroecosystems: A Field Study, John C. Stickney

Theses and Dissertations--Biosystems and Agricultural Engineering

Nanopesticides are an emerging class of pesticides associated with engineered nanoparticles that provide agricultural and environmental benefits. However, nanopesticide fate, transport, and persistence remain poorly understood. As do their potential impacts on the health of agroecosystems and influence on nutrient release. Therefore, this project investigated the fate, transport, and persistence of two widely available nanopesticides, an insecticide, nano-imidacloprid, and a fungicide, nano-copper hydroxide. The fate and transport in runoff water and soils of these nanopesticides, as well as their impacts on nutrients retention, were assessed through a field plot study over 2 growing seasons. Runoff from rainfall events and soil …

Safety Monitoring & Intelligent Power Distribution Systems For Compact Agricultural Equipment, Mason O. Bradley

Theses and Dissertations--Biosystems and Agricultural Engineering

The agricultural industry continues to be one of the most dangerous career fields in the United States of America with compact agricultural equipment being a common source for casualties in the industry. The primary focus of this work is to develop new technology that improves operational safety of compact agricultural equipment through the use of low-cost embedded electronics. This work presents the design of three systems in alignment with this goal.

The first system aims to integrate electrical intelligence and passive safety monitoring into the power distribution system of a small piece of agricultural equipment. The system known as the …