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Articles 2881 - 2910 of 52418

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Introducing Social Determinants Of Health To The Alzheimer's Disease Research Center Network, Megan Zuelsdorff, Erin L. Abner, Joyce E. Balls-Berry, Gregory A. Jicha, Serggio Lanata, Gladys E. Maestre, Monica Rosselli, Shana D. Stites, Rachel A. Whitmer, Consuelo H. Wilkins, Lisa L. Barnes May 2025

Introducing Social Determinants Of Health To The Alzheimer's Disease Research Center Network, Megan Zuelsdorff, Erin L. Abner, Joyce E. Balls-Berry, Gregory A. Jicha, Serggio Lanata, Gladys E. Maestre, Monica Rosselli, Shana D. Stites, Rachel A. Whitmer, Consuelo H. Wilkins, Lisa L. Barnes

Epidemiology and Environmental Health Faculty Publications

The Alzheimer's Disease Research Centers (ADRCs) consortium represents a critical locus of research on Alzheimer's disease and related disorders (ADRD) prevention, diagnosis, and intervention. Through the National Alzheimer's Coordinating Center's (NACC) standardized protocol, the Uniform Data Set (UDS), ADRCs have collected rich, harmonizable clinical and cognitive data. However, the collection of social data has been sparse and Center specific, constraining ADRD science that addresses research priorities on social determinants of health (SDOH) and health equity. Capitalizing on the transition to a revised UDS version 4, an interdisciplinary committee representing 10 ADRCs reviewed the literature and instrumentation, ultimately creating a brief …

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Pharmacokinetic Modeling And Model-Based Hypothesis Generation For Dose Optimization Of Clonidine In Neonates With Neonatal Opioid Withdrawal Syndrome, Fei Tang, Chee M. Ng, Jamie Horn, Henrietta S. Bada, Markos Leggas May 2025

Pharmacokinetic Modeling And Model-Based Hypothesis Generation For Dose Optimization Of Clonidine In Neonates With Neonatal Opioid Withdrawal Syndrome, Fei Tang, Chee M. Ng, Jamie Horn, Henrietta S. Bada, Markos Leggas

Epidemiology and Environmental Health Faculty Publications

The No-POPPY study (NCT03396588), a double-blind, randomized trial compared morphine with clonidine therapy for neonatal opioid withdrawal syndrome (NOWS) and found that the duration of treatment was similar across groups. This is significant because perinatal use of morphine has the potential for neurodevelopmental consequences. Still, the clonidine group reached symptom stabilization (Finnegan score (FS) < 8) later than the morphine group and had a more significant number of patients who required adjunct therapy. However, the mean FS was consistently lower in the clonidine group after day 6. This prompted us to use pharmacokinetic (PK) and parametric time-to-event (TTE) modeling to simulate dosage schedules that may decrease the time to stabilization and reduce the need for adjunct therapy. Population PK (popPK) analysis was conducted, and the final model was a one-compartment model with first-order absorption and elimination, incorporating allometric scaling and age effect on apparent clearance (CL/F) and apparent volume (V/F). The population estimates for CL/F and V/F were 13.6 L/h/70 kg and 416 L/70 kg, respectively, similar to the reported values. A Weibull model described the TTE data best, followed by incorporating predicted average concentrations to yield the final Weibull accelerated failure time model. Simulations of dosing strategies showed that increasing both the starting and maximum doses could potentially shorten the time to stabilization, and thus, length of treatment and hospital stay. Given the hypothesis-generating nature of this analysis, the recommended dosing regimens should be tested prospectively to evaluate their benefits.

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Hypercoagulable And Autoimmune Factors In Patients With Moyamoya Syndrome, Justin F. Fraser, Min Jian Hou Ma, Christopher Mclouth, Laura Whitnel, Jessica Lasslo, David Dornbos Iii, Jill Roberts May 2025

Hypercoagulable And Autoimmune Factors In Patients With Moyamoya Syndrome, Justin F. Fraser, Min Jian Hou Ma, Christopher Mclouth, Laura Whitnel, Jessica Lasslo, David Dornbos Iii, Jill Roberts

Neuroscience Research Data

Moyamoya is a progressive cerebrovascular condition leading to stenosis of the internal carotid arteries (ICA) and formation of abnormal vasculature at the base of the brain. Patients ultimately suffer from ischemic and/or hemorrhagic stroke. In Kentucky, patients often present with comorbidities, which classifies them as having moyamoya syndrome (MMS). In this prospective observational study, we measured coagulable- and autoimmune-related factors in MMS subjects to identify possible therapeutic targets and help inform clinical care. Venous blood samples were collected from adult subjects diagnosed with MMS and analyzed by the UK Healthcare Clinical Laboratory. Blood samples, demographics, and medical records were obtained …

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Beyond The Legislature’S Website: Strategies And Tools For State Legislative Research That Informs Advocacy Work, Jennifer Horne May 2025

Beyond The Legislature’S Website: Strategies And Tools For State Legislative Research That Informs Advocacy Work, Jennifer Horne

Library Faculty and Staff Publications

As the nation’s state legislatures increasingly decide the issues that affect citizens most directly, the ability to identify, track, and understand state legislation is key to successful advocacy efforts. While all state legislative websites provide the status and authoritative text of the bill, this may be insufficient to understand what the bill seeks to do and why the change is being proposed. To find plain English summaries and necessary context and background information on proposed bills, advocates may also need to find appropriate news sources and identify key stakeholders—those who care the most about the bill or are the most …

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Genetic Identification Of Lamprey Genera And Anadromous Ecotypes In Watersheds Of The Northeastern Pacific Ocean, G. S. Silver, R. T. Lampman, N. Percival, N. Timoshevskaya, J. J. Smith, K. T. Bentley, J. Wade, S. R. Narum, J. E. Hess May 2025

Genetic Identification Of Lamprey Genera And Anadromous Ecotypes In Watersheds Of The Northeastern Pacific Ocean, G. S. Silver, R. T. Lampman, N. Percival, N. Timoshevskaya, J. J. Smith, K. T. Bentley, J. Wade, S. R. Narum, J. E. Hess

Biology Faculty Publications

Nonparasitic, nonmigratory Western Brook Lamprey (WBL; Lampetra ayresii), and parasitic, anadromous Western River Lamprey (WRL; L. ayresii) are sympatric lampreys that likely represent different life history variations of a single species. Novel genetic tools are critical for differentiating WBL and WRL, whose larvae preclude morphological identification (ID) and will enable comprehensive assessment of imperiled native lampreys of the Northeastern Pacific (including WBL, WRL, and Pacific Lamprey, Entosphenus tridentatus). We developed 47 candidate single nucleotide polymorphism (SNP) markers using whole genome resequencing of WBL (N = 24) and WRL (N = 15) from Ksi Ts'oohl Ts'ap Creek (Nass River, British Columbia, …

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On The Optimal Design Of Coreless Afpm Machines With Halbach Array Rotors For Electric Aircraft Propulsion, Matin Vatani, John F. Eastham, Xiaoze Pei, Dan M. Ionel May 2025

On The Optimal Design Of Coreless Afpm Machines With Halbach Array Rotors For Electric Aircraft Propulsion, Matin Vatani, John F. Eastham, Xiaoze Pei, Dan M. Ionel

Electrical and Computer Engineering Graduate Research

This paper provides a comprehensive analysis of the winding factor and winding harmonic distribution in coreless stator axial flux permanent magnet (AFPM) machines. The topology of this type of machine and its similarities to conventional cored AFPM machines are discussed. Popular pole/coil number combinations in coreless stator AFPM machines are identified and analyzed in detail. It is demonstrated that the winding factor in these machines varies with radius and can be expressed as the product of the conductor distribution factor, pitch factor, and coil group factor. The formulas for each factor are derived and presented in multiple forms based on …

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Hybrid Fea And Meta-Modeling For De Optimization Of A Highly Saturated Spoke Ipm, Oluwaseun A. Badewa, Marcelo Silva, Rosemary E. Alden, Pedram Asef, Dan M. Ionel May 2025

Hybrid Fea And Meta-Modeling For De Optimization Of A Highly Saturated Spoke Ipm, Oluwaseun A. Badewa, Marcelo Silva, Rosemary E. Alden, Pedram Asef, Dan M. Ionel

Electrical and Computer Engineering Graduate Research

This paper introduces a novel approach for high performance electric motor design that combines machine learning (ML)-based meta-modeling with a differential evolution (DE) optimization algorithm. The method leverages finite element analysis (FEA) results to train the ML meta-model, enabling efficient design optimization for high-power density cored machines, such as spoke interior permanent magnet motors (IPM), which exhibit complex nonlinearities and saturation effects. This hybrid ML-DE framework seeks to provide an alternative for physics-based electric motor design and optimization, offering significant reductions in computational effort while maintaining accuracy. The meta-model’s accuracy in capturing the nonlinear relationships between design parameters, core losses, …

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Optimal Design Of Coreless Axial Flux Pm Machines Using A Hybrid Machine Learning And Differential Evolution Method, Matin Vatani, David R. Stewart, Pedram Asef, Dan M. Ionel May 2025

Optimal Design Of Coreless Axial Flux Pm Machines Using A Hybrid Machine Learning And Differential Evolution Method, Matin Vatani, David R. Stewart, Pedram Asef, Dan M. Ionel

Electrical and Computer Engineering Graduate Research

Coreless stator axial flux permanent magnet (AFPM) machines require computationally intensive three dimensional finite element analysis (FEA) for accurate performance evaluation, making optimization time-consuming and impractical for large-scale design studies. This paper presents a hybrid optimization approach that integrates differential evolution (DE) with artificial neural networks (ANNs) to accelerate the optimization of coreless AFPM machines. In this method, DE driven FEA simulations generate a dataset used to train an ANN surrogate model, significantly reducing reliance on direct FEA computations. The effectiveness of this approach is demonstrated through a multi-objective DE optimization, where the ANN’s predictions are validated against FEA results. …

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Winding Factors And Harmonics Of Coreless Axial Flux Pm Machines, Matin Vatani, John F. Eastham, Xiaoze Pei, Dan M. Ionel May 2025

Winding Factors And Harmonics Of Coreless Axial Flux Pm Machines, Matin Vatani, John F. Eastham, Xiaoze Pei, Dan M. Ionel

Electrical and Computer Engineering Graduate Research

This paper provides a comprehensive analysis of the winding factor and winding harmonic distribution in coreless stator axial flux permanent magnet (AFPM) machines. The topology of this type of machine and its similarities to conventional cored AFPM machines are discussed. Popular pole/coil number combinations in coreless stator AFPM machines are identified and analyzed in detail. It is demonstrated that the winding factor in these machines varies with radius and can be expressed as the product of the conductor distribution factor, pitch factor, and coil group factor. The formulas for each factor are derived and presented in multiple forms based on …

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Probing The Critical Element Chemistry Of Coal-Combustion Fly Ash, Debora Berti, John G. Groppo, Prakash Joshi, Dorin V. Preda, David P. Gamliel, Todd Beers, Michael Schrock, Shelley D. Hopps, Tonya D. Morgan, Bernd Zechmann, James C. Hower May 2025

Probing The Critical Element Chemistry Of Coal-Combustion Fly Ash, Debora Berti, John G. Groppo, Prakash Joshi, Dorin V. Preda, David P. Gamliel, Todd Beers, Michael Schrock, Shelley D. Hopps, Tonya D. Morgan, Bernd Zechmann, James C. Hower

Earth and Environmental Sciences Faculty Publications

Along with the principal rare earth (REE) minerals such as monazite, xenotime, and bastnasite, Y-and REE-bearing zircon and associated minerals survive the combustion process and are found in coal-combustion fly ash. Beneficiated fly ash from a power plant burning an eastern-Kentucky-sourced coal blend was found to have zircon (ZrSiO4), baddeleyite (ZrO2), fergusonite (YNbO4), yttriaite (Y2O3), and xenotime (YPO4). Previous studies of the same fly had also identified monazite with a broad REE suite. Scanning electron microscopy–electron dispersive spectroscopy (EDS) and transmission electron microscopy (TEM)–EDS as well as other …

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Resistance Exercise And Mechanical Overload Upregulate Vimentin For Skeletal Muscle Remodeling, Joshua S. Godwin, J. Max Michel, Cleiton A. Libardi, Andreas N. Kavazis, Christopher S. Fry, Andrew D. Frugé, Mariah Mccashland, Ivan J. Vechetti, John J. Mccarthy, C. Brooks Mobley, Michael D. Roberts May 2025

Resistance Exercise And Mechanical Overload Upregulate Vimentin For Skeletal Muscle Remodeling, Joshua S. Godwin, J. Max Michel, Cleiton A. Libardi, Andreas N. Kavazis, Christopher S. Fry, Andrew D. Frugé, Mariah Mccashland, Ivan J. Vechetti, John J. Mccarthy, C. Brooks Mobley, Michael D. Roberts

Physiology Faculty Publications

We adopted a proteomic and follow-through approach to investigate how mechanical overload (MOV) potentially affects novel targets in skeletal muscle, and how a perturbation in this response could potentially affect the adaptive response. First, we determined that 10 wk of resistance training in 15 college-aged females increased sarcolemmal-associated protein content (+10.1%, P < 0.05). Sarcolemmal protein isolates were then queried using mass spectrometry-based proteomics, ∼10% (38/387) of proteins putatively associated with the sarcolemma or extracellular matrix (ECM) were upregulated (>1.5-fold, P < 0.05), and one target (intermediate filament vimentin; VIM) warranted further investigation due to its correlation to myofiber hypertrophy (r = 0.652, P = 0.009). VIM expression was then examined in 4-mo-old C57BL/6J mice following 10 and 20 days of plantaris MOV via synergist ablation. Relative to Sham (control) mice, VIM mRNA and protein content was significantly higher in MOV mice, and immunohistochemistry indicated that VIM predominantly resided in the ECM. MOV experiments were replicated in Pax7-DTA (satellite cell depleted) mice, which reduced VIM in the ECM by ∼74%. A third MOV experiment was performed in C57BL/6 mice intramuscularly injected with either AAV9-scrambled (control) or AAV9-VIM-shRNA. Although VIM-shRNA mice possessed lower VIM in the ECM (∼45%), plantaris masses in response to MOV were similar between groups. However, VIMshRNA mice possessed smaller and more centrally nucleated MyHCemb-positive fibers in response to MOV. In summary, skeletal muscle VIM appears to be enriched in the ECM following MOV, satellite cells may regulate its expression, and a disruption in expression during MOV leads to an excessive regenerative phenotype.

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Therapeutic Applications Of A Novel Humanized Monoclonal Antibody Targeting Chemokine Receptor Ccr9 In Pancreatic Cancer, Hannah G. Mcdonald, Anna M. Reagan, Charles J. Bailey, Mei Gao, Muqiang Gao, Angelica L. Solomon, Michael J. Cavnar, Prakash Pandalai, Mautin Barry-Hundeyin, Megan Harper, Justin A. Rueckert, Ángela Turrero, Araceli Tobio, Anxo Vidal, Daniel Roca-Lema, Elia Álvarez-Coiradas, Pablo Garrido, Laureano Simón, Joseph Kim May 2025

Therapeutic Applications Of A Novel Humanized Monoclonal Antibody Targeting Chemokine Receptor Ccr9 In Pancreatic Cancer, Hannah G. Mcdonald, Anna M. Reagan, Charles J. Bailey, Mei Gao, Muqiang Gao, Angelica L. Solomon, Michael J. Cavnar, Prakash Pandalai, Mautin Barry-Hundeyin, Megan Harper, Justin A. Rueckert, Ángela Turrero, Araceli Tobio, Anxo Vidal, Daniel Roca-Lema, Elia Álvarez-Coiradas, Pablo Garrido, Laureano Simón, Joseph Kim

Markey Cancer Center Faculty Publications

The relative failure of immune checkpoint inhibitors in pancreatic ductal adenocarcinoma (PDAC) despite having a dense, immunosuppressive tumor microenvironment highlights the need to target alternate/escape pathways. We have previously examined C–C chemokine receptor type 9 (CCR9) as a candidate immune checkpoint and developed a targeted, humanized monoclonal antibody (SRB2). Cytotoxicity of SRB2 was evaluated in vitro and in vivo. CCR9 expression on PDAC cells/tissues, immune components of patient-derived organoids (PDOs), and antibody-dependent cell-mediated cytotoxicity were examined. In PANC-1 and MIA PaCa-2 cell lines, we demonstrated highest CCR9 expression; however, no direct cytotoxic effect was observed with SRB2 treatment. In PANC-1 …

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Correction To: Putting Biomonitors To Work: Native Moss As A Screening Tool For Solid Waste Incineration, Sarah E. Jovan, Eleonore Jacobson, Jason M. Unrine, Nasser Jalili‑Jahani, Bruce Mccune May 2025

Correction To: Putting Biomonitors To Work: Native Moss As A Screening Tool For Solid Waste Incineration, Sarah E. Jovan, Eleonore Jacobson, Jason M. Unrine, Nasser Jalili‑Jahani, Bruce Mccune

Markey Cancer Center Faculty Publications

Correction to: Environ Monit Assess (2024) 196:1177 https://doi.org/10.1007/s10661-024-13354-y

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An Agrin-Yap/Taz Rigidity Sensing Module Drives Egfr-Addicted Lung Tumorigenesis., Reza Bayat Mokhtari, Divyaleka Sampath, Paige Eversole, Melissa Ong Yu Lin, Dmitriy A. Bosykh, Gandhi T. K. Boopathy, Aravind Sivakumar, Cheng-Chun Wang, Ramesh Kumar, Joe Yeong Poh Sheng, Ellen Karasik, Barbara A. Foster, Han Yu, Xiang Ling, Wenjie Wu, Fengzhi Li, Zoë Weaver Ohler, Christine Fillmore Brainson, David W. Goodrich, Wanjin Hong, Sayan Chakraborty May 2025

An Agrin-Yap/Taz Rigidity Sensing Module Drives Egfr-Addicted Lung Tumorigenesis., Reza Bayat Mokhtari, Divyaleka Sampath, Paige Eversole, Melissa Ong Yu Lin, Dmitriy A. Bosykh, Gandhi T. K. Boopathy, Aravind Sivakumar, Cheng-Chun Wang, Ramesh Kumar, Joe Yeong Poh Sheng, Ellen Karasik, Barbara A. Foster, Han Yu, Xiang Ling, Wenjie Wu, Fengzhi Li, Zoë Weaver Ohler, Christine Fillmore Brainson, David W. Goodrich, Wanjin Hong, Sayan Chakraborty

Markey Cancer Center Faculty Publications

Despite epidermal growth factor receptor (EGFR) is a pivotal oncogene for several cancers, including lung adenocarcinoma (LUAD), how it senses extracellular matrix (ECM) rigidity remain elusive in the context of the increasing role of tissue rigidity on various hallmarks of cancer development. Here it is shown that EGFR dictates tumorigenic agrin expression in lung cancer cell lines, genetically engineered EGFR-driven mouse models, and human specimens. Agrin expression confers substrate stiffness-dependent oncogenic attributes to EGFR-reliant cancer cells. Mechanistically, agrin mechanoactivates EGFR through epidermal growth factor (EGF)-dependent and independent modes, thereby sensitizing its activity toward localized cancer cell-ECM adherence and bulk rigidity …

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Support Effects On Fe- Or Cu-Promoted Ni Catalysts Used In The Catalytic Deoxygenation Of Tristearin To Fuel-Like Hydrocarbons, Great C. Umenweke, Robert Pace, Thomas Récalt, Olivier Heintz, Gilles Caboche, Eduardo Santillan-Jimenez May 2025

Support Effects On Fe- Or Cu-Promoted Ni Catalysts Used In The Catalytic Deoxygenation Of Tristearin To Fuel-Like Hydrocarbons, Great C. Umenweke, Robert Pace, Thomas Récalt, Olivier Heintz, Gilles Caboche, Eduardo Santillan-Jimenez

Chemistry Faculty Publications

Previous studies have shown that fats, oils, and greases (FOG) can be deoxygenated to fuel-like hydrocarbons over inexpensive alumina-supported Ni catalysts promoted with Cu or Fe to afford excellent yields of renewable diesel (RD). In this study, supports other than alumina—namely, SiO2-Al2O3, Ce0.8Pr0.2O2, and ZrO2—were investigated to develop catalysts showing improved RD yields and resistance to coke-induced deactivation relative to Al2O3-supported catalysts. Results showed that catalysts supported on Ce0.8Pr0.2O2 and ZrO2 outperformed SiO2-Al2 …

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Temporal Immune Profiling In The Cerebrospinal Fluid And Blood Of Patients With Aneurysmal Subarachnoid Hemorrhage, Thomas A. Ujas, Katie L. Anderson, Jenny Lutshumba, Samantha N. Hart, Jadwiga Turchan-Cholewo, Kevin W. Hatton, Adam D. Bachstetter, Barbara S. Nikolajczyk, Ann M. Stowe May 2025

Temporal Immune Profiling In The Cerebrospinal Fluid And Blood Of Patients With Aneurysmal Subarachnoid Hemorrhage, Thomas A. Ujas, Katie L. Anderson, Jenny Lutshumba, Samantha N. Hart, Jadwiga Turchan-Cholewo, Kevin W. Hatton, Adam D. Bachstetter, Barbara S. Nikolajczyk, Ann M. Stowe

Surgery Faculty Publications

Delayed cerebral ischemia (DCI) is a significant complication of aneurysmal subarachnoid hemorrhage (aSAH). This study profiled immune responses after aSAH and evaluated their association with DCI onset. Twelve aSAH patients were enrolled. Leukocyte populations and cytokine levels were analyzed in cerebrospinal fluid (CSF) and peripheral blood (PB) on days 3, 5, 7, 10, and 14 post-aSAH. PB mononuclear cells (PBMCs) were collected, and their cytokine production quantified following stimulation. Mixed-effects models reveal distinct immune cell dynamics in CSF compared with blood. Monocyte/macrophage numbers continue to increase in both CSF and PBMCs for days post-aSAH. CD4+ human leukocyte antigen II+ …

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Training, Validating, And Testing Machine Learning Prediction Models For Endometrial Cancer Recurrence, Jesus Gonzalez Bosquet, Andrew Polio, Erin George, Ahmad A. Tarhini, Casey M. Cosgrove, Marilyn S. Huang, Bradley Corr, Aliza L. Leiser, Bodour Salhia, Kathleen Darcy, Christopher M. Tarney, Rob L. Dood, Lauren E. Dockery, Stephen B. Edge, Michael J. Cavnar, Lisa Landrum, Rob J. Rounbehler, Michelle Churchman, Vincent M. Wagner May 2025

Training, Validating, And Testing Machine Learning Prediction Models For Endometrial Cancer Recurrence, Jesus Gonzalez Bosquet, Andrew Polio, Erin George, Ahmad A. Tarhini, Casey M. Cosgrove, Marilyn S. Huang, Bradley Corr, Aliza L. Leiser, Bodour Salhia, Kathleen Darcy, Christopher M. Tarney, Rob L. Dood, Lauren E. Dockery, Stephen B. Edge, Michael J. Cavnar, Lisa Landrum, Rob J. Rounbehler, Michelle Churchman, Vincent M. Wagner

Surgery Faculty Publications

PURPOSEEndometrial cancer (EC) is the most common gynecologic cancer in the United States with rising incidence and mortality. Despite optimal treatment, 15%-20% of all patients will recur. To better select patients for adjuvant therapy, it is important to accurately predict patients at risk for recurrence. Our objective was to train, validate, and test models of EC recurrence using lasso regression and other machine learning (ML) and deep learning (DL) analytics in a large, comprehensive data set.METHODSData from patients with EC were downloaded from the Oncology Research Information Exchange Network database and stratified into low risk, The International Federation of Gynecology …

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Outcomes Associated With Absent Blood Product Utilization In Jehovah’S Witness Patients Compared To The Standard Of Care In Cardiac Surgery, Nathan Fields, Ayesha Ather, Dan Davenport, Sadiq Ahmed, Michael Sekela May 2025

Outcomes Associated With Absent Blood Product Utilization In Jehovah’S Witness Patients Compared To The Standard Of Care In Cardiac Surgery, Nathan Fields, Ayesha Ather, Dan Davenport, Sadiq Ahmed, Michael Sekela

Surgery Faculty Publications

Introduction: For Jehovah’s Witness (JW) patients requiring cardiac surgery, various strategies such as preoperative use of erythropoietin stimulating agents (ESAs), intravenous iron (IVI), and non-pharmacologic interventions have emerged to prevent complications from blood loss given transfusion is not acceptable in this population. Methods: Retrospective case-control of cardiac surgeries performed by the same surgeon between 1/1/2011 and 8/30/2021. JW patients were matched to non-JW who received blood products and non-JW who did not receive blood products on a 1:2:2 basis. Patients were matched on procedure, age, gender, and Society of Thoracic Surgeons morbidity score. Eligible patients were aged >18 years and …

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Shared Roles Of Immune And Stromal Cells In The Pathogenesis Of Human Bronchiolitis Obliterans Syndrome, Patrick W. Mellors, Ana N. Lange, Bruno Casino Remondo, Maksim Shestov, Joseph D. Planer, Andrew R. Peterson, Yun Ying, Su Zhou, Jason D. Christie, Joshua M. Diamond, Edward Cantu, Maria C. Basil, Saar Gill May 2025

Shared Roles Of Immune And Stromal Cells In The Pathogenesis Of Human Bronchiolitis Obliterans Syndrome, Patrick W. Mellors, Ana N. Lange, Bruno Casino Remondo, Maksim Shestov, Joseph D. Planer, Andrew R. Peterson, Yun Ying, Su Zhou, Jason D. Christie, Joshua M. Diamond, Edward Cantu, Maria C. Basil, Saar Gill

Surgery Faculty Publications

Bronchiolitis obliterans syndrome (BOS) is a progressive, fatal obstructive lung disease that occurs following lung transplant, where it is termed chronic lung allograft dysfunction BOS (CLAD-BOS), or as the primary manifestation of pulmonary chronic graft versus host disease (cGVHD-BOS) following allogeneic hematopoietic stem cell transplant. Disease pathogenesis is poorly understood; however, chronic alloreactivity is common to both conditions, suggesting a shared pathophysiology. We performed single-cell RNA-Seq (scRNA-Seq) on explanted human lungs from 4 patients with CLAD-BOS, 3 patients with cGVHD-BOS, and 3 deceased controls to identify cell types, genes, and pathways enriched in BOS to better understand disease mechanisms. In …

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Reproductive Performance And Milk Composition Of Sows Fed Diets Supplemented With An Immunomodulator, Mark J. Estienne, Jung W. Lee, R. Tyler Niblett, Brooke D. Humphrey, H. James Monegue, Merlin D. Lindemann May 2025

Reproductive Performance And Milk Composition Of Sows Fed Diets Supplemented With An Immunomodulator, Mark J. Estienne, Jung W. Lee, R. Tyler Niblett, Brooke D. Humphrey, H. James Monegue, Merlin D. Lindemann

Animal and Food Sciences Faculty Publications

A cooperative study involving 189 litters from 114 sows (initial BW of 200.8 ± 37.1 kg) at two experiment stations was conducted to investigate the effects of dietary supplementation with OmniGen-AF (OG) (Phibro Animal Health Co., Teaneck, NJ, USA), a nutritional product formulated to improve immune function of animals, on sow reproductive performance and milk composition. Dietary treatments were (1) corn–soybean meal-based control diets or (2) control diets supplemented with OG at 0.75% (~9 g of OG/100 kg BW/d). Supplementation of diets with OG resulted in lesser (p < 0.05) BW changes of sows during lactation (−12.1 vs. −8.2 kg). Litter sizes for control and OG-fed sows were similar, but sows fed OG-based diets had greater (p < 0.05) litter weight for total born (18.3 vs. 19.3 kg) and weaned (63.2 vs. 67.0 kg) and lactation litter gain (47.8 vs. 50.7 kg). Lactation feed intake for the controls and OG-fed sows (5.32 vs. 5.52 kg/d, respectively) did not differ. Supplementing diets with OG increased lactose content (5.78 vs. 5.84%; p = 0.05) and reduced protein content (4.77 vs. 4.68%; p = 0.04) in sow milk. In conclusion, dietary supplementation with OG at 0.75% reduced weight loss during lactation and improved litter weights with marginal effects on the milk composition of sows.

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Performance, Energy, And Nutrient Utilization Benefits With Exogenous Enzyme Supplementation Of Wheat-Soybean Meal-Based Diets Fed To 22-Day-Old Broiler Chickens, M. M. Bauer, T. Ao, J. P. Jacob, M. J. Ford, A. J. Pescatore, R. F. Power, S. A. Adedokun May 2025

Performance, Energy, And Nutrient Utilization Benefits With Exogenous Enzyme Supplementation Of Wheat-Soybean Meal-Based Diets Fed To 22-Day-Old Broiler Chickens, M. M. Bauer, T. Ao, J. P. Jacob, M. J. Ford, A. J. Pescatore, R. F. Power, S. A. Adedokun

Animal and Food Sciences Faculty Publications

This study was designed to investigate the performance, bone quality, and nutrient and energy utilization in broiler chickens fed wheat-soybean meal-based diets with a dietary multi-enzyme complex. The positive control (PC) diet met or exceeded energy and nutrient needs, while the negative control (NC) diet was formulated to contain 3.0, 16.7, and 33.3% less of AMEn, Ca, and avP, respectively. Enzyme complex was supplemented to the NC diet at 0, 150, 200, or 250 mg/kg during the pre-starter (d 0-9) and starter (d 9-22) phases. A total of 300 d-old male broiler chicks were assigned to the 5 treatments in …

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Sex- And Tissue-Specific Effects Of Leukemia Inhibitory Factor On Mitochondrial Bioenergetics Following Ischemic Stroke, Hemendra J. Vekaria, Sarah J. Shelley, Sarah J. Messmer, Prashant D. Kunjadia, Christopher J. Mclouth, Patrick G. Sullivan, Justin F. Fraser, Keith R. Pennypacker, Chirayu D. Pandya May 2025

Sex- And Tissue-Specific Effects Of Leukemia Inhibitory Factor On Mitochondrial Bioenergetics Following Ischemic Stroke, Hemendra J. Vekaria, Sarah J. Shelley, Sarah J. Messmer, Prashant D. Kunjadia, Christopher J. Mclouth, Patrick G. Sullivan, Justin F. Fraser, Keith R. Pennypacker, Chirayu D. Pandya

Biostatistics Faculty Publications

Oxidative stress due to increased reactive oxygen species (ROS) formation and/or inflammation is considered to play an important role in ischemic stroke injury. Leukemia inhibitory factor (LIF) has been shown to protect both oligodendrocytes and neurons from ischemia by upregulating endogenous anti-oxidants, though the effect of ischemia and the protective role of LIF treatment in mitochondrial function have not been studied. The goal of this study was to determine whether LIF protects ischemia-induced altered mitochondrial bioenergetics in reproductively senescent aged rats of both sexes (≥18 months old), approximately equivalent to the average age of human stroke patients. Animals were euthanized …

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Glycosylation Of Serine/Threonine-Rich Intrinsically Disordered Regions Of Membrane-Associated Proteins In Streptococci., Mohammad M Rahman, Svetlana Zamakhaeva, Jeffrey S. Rush, Catherine T Chaton, Cameron W Kenner, Yin Mon Hla, Ho-Ching Tiffany Tsui, Vladimir N Uversky, Malcolm E Winkler, Konstantin V Korotkov, Natalia Korotkova Apr 2025

Glycosylation Of Serine/Threonine-Rich Intrinsically Disordered Regions Of Membrane-Associated Proteins In Streptococci., Mohammad M Rahman, Svetlana Zamakhaeva, Jeffrey S. Rush, Catherine T Chaton, Cameron W Kenner, Yin Mon Hla, Ho-Ching Tiffany Tsui, Vladimir N Uversky, Malcolm E Winkler, Konstantin V Korotkov, Natalia Korotkova

Molecular and Cellular Biochemistry Faculty Publications

Proteins harboring intrinsically disordered regions (IDRs) lacking stable secondary or tertiary structures are abundant across the three domains of life. These regions have not been systematically studied in prokaryotes. Here, our genome-wide analysis identifies extracytoplasmic serine/threonine-rich IDRs in several biologically important membrane-associated proteins in streptococci. We demonstrate that these IDRs are glycosylated with glucose by glycosyltransferases GtrB and PgtC2 in Streptococcus pyogenes and Streptococcus pneumoniae, and with N-acetylgalactosamine by a Pgf-dependent mechanism in Streptococcus mutans. The absence of glycosylation leads to a defect in biofilm formation under ethanol-stressed conditions in S. mutans. We link this phenotype to the C-terminal IDR …

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Pparα Regulates Er–Lipid Droplet Protein Calsyntenin-3Β To Promote Ketogenesis In Hepatocytes, Lauren F. Uchiyama, Alexander Nguyen, Kevin Qian, Liujuan Cui, Khoi T. Pham, Xu Xiao, Yajing Gao, Yuta Shimanaka, Marcus J. Tol, Laurent Vergnes, Karen Reue, Peter Tontonoz Apr 2025

Pparα Regulates Er–Lipid Droplet Protein Calsyntenin-3Β To Promote Ketogenesis In Hepatocytes, Lauren F. Uchiyama, Alexander Nguyen, Kevin Qian, Liujuan Cui, Khoi T. Pham, Xu Xiao, Yajing Gao, Yuta Shimanaka, Marcus J. Tol, Laurent Vergnes, Karen Reue, Peter Tontonoz

Physiology Faculty Publications

Ketogenesis requires fatty acid flux from intracellular (lipid droplets) and extrahepatic (adipose tissue) lipid stores to hepatocyte mitochondria. However, whether interorganelle contact sites regulate this process is unknown. Recent studies have revealed a role for Calsyntenin-3β (CLSTN3β), an endoplasmic reticulum–lipid droplet contact site protein, in the control of lipid utilization in adipose tissue. Here, we show that Clstn3b expression is induced in the liver by the nuclear receptor PPARα in settings of high lipid utilization, including fasting and ketogenic diet feeding. Hepatocyte-specific loss of CLSTN3β in mice impairs ketogenesis independent of changes in PPARα activation. Conversely, hepatic overexpression of CLSTN3β …

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Light-Driven Co2 Reduction With Substituted Imidazole-Pyridine Re Catalysts Favoring Formic Acid Production, Ryan Chafin, Majharul Islam Sujan, Sean Parkin, Jonah W. Jurss, Aron J. Huckaba Apr 2025

Light-Driven Co2 Reduction With Substituted Imidazole-Pyridine Re Catalysts Favoring Formic Acid Production, Ryan Chafin, Majharul Islam Sujan, Sean Parkin, Jonah W. Jurss, Aron J. Huckaba

Chemistry Faculty Publications

Removing carbon dioxide from the atmosphere is an attractive way to mitigate the greenhouse gas effect that contributes to climate change. A series of donor-pi (D-π), acceptor-pi (A-π), and π Re(i) pyridyl imidazole complexes have been synthesized and examined under photocatalytic conditions for the photocatalytic reduction of CO2. The catalytic activity of the complexes was further supported by cyclic voltammetry through the presence of a catalytic current under CO2 atmosphere. The D-π, π, and A-π complexes were studied to elucidate the effects of incorporating conjugated electron donating vs. withdrawing groups on the catalytic rates and product selectivity. …

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Pyroptosis Of Pulmonary Fibroblasts And Macrophages Through Nlrc4 Inflammasome Leads To Acute Respiratory Failure, Yan Zhang, Guoying Zhang, Brittany Dong, Ankit Pandeya, Jian Cui, Samuel Dos Santos Valenca, Ling Yang, Jiaqian Qi, Zhuodong Chai, Congqing Wu, Daniel Kirchhofer, Toshihiko Shiroishi, Fadi Khasawneh, Min Tao, Feng Shao, Christopher M. Waters, Yinan Wei, Zhenyu Li Apr 2025

Pyroptosis Of Pulmonary Fibroblasts And Macrophages Through Nlrc4 Inflammasome Leads To Acute Respiratory Failure, Yan Zhang, Guoying Zhang, Brittany Dong, Ankit Pandeya, Jian Cui, Samuel Dos Santos Valenca, Ling Yang, Jiaqian Qi, Zhuodong Chai, Congqing Wu, Daniel Kirchhofer, Toshihiko Shiroishi, Fadi Khasawneh, Min Tao, Feng Shao, Christopher M. Waters, Yinan Wei, Zhenyu Li

Chemistry Faculty Publications

The NAIP/NLRC4 inflammasome plays a pivotal role in the defense against bacterial infections, with its in vivo physiological function primarily recognized as driving inflammation in immune cells. Acute lung injury (ALI) is a leading cause of mortality in sepsis. In this study, we identify that the NAIP/NLRC4 inflammasome is highly expressed in both macrophages and pulmonary fibroblasts and that pyroptosis of these cells plays a critical role in lung injury. Mice challenged with gram-negative bacteria or flagellin developed lethal lung injury, characterized by reduced blood oxygen saturation, disrupted lung barrier function, and escalated inflammation. Flagellin-induced lung injury was protected in …

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Targeting Mtor In Myeloid Cells Prevents Infection-Associated Inflammation, Yohana C. Toner, Jazz Munitz, Geoffrey Prevot, Judit Morla-Folch, William Wang, Yuri Van Elsas, Bram Priem, Jeroen Deckers, Tom Anbergen, Thijs J. Beldman, Eliane E.S. Brechbühl, Muhammed D. Aksu, Athanasios Ziogas, Sebastian A. Sarlea, Mumin Ozturk, Zhenhua Zhang, Wenchao Li, Yang Li, Alexander Maier, Jessica C. Fernandes, Glenn A.O. Cremers, Bas Van Genabeek, Joost H.C.M. Kreijtz, Esther Lutgens, Niels P. Riksen, Henk M. Janssen, Serge H.M. Söntjens, Freek J.M. Hoeben Apr 2025

Targeting Mtor In Myeloid Cells Prevents Infection-Associated Inflammation, Yohana C. Toner, Jazz Munitz, Geoffrey Prevot, Judit Morla-Folch, William Wang, Yuri Van Elsas, Bram Priem, Jeroen Deckers, Tom Anbergen, Thijs J. Beldman, Eliane E.S. Brechbühl, Muhammed D. Aksu, Athanasios Ziogas, Sebastian A. Sarlea, Mumin Ozturk, Zhenhua Zhang, Wenchao Li, Yang Li, Alexander Maier, Jessica C. Fernandes, Glenn A.O. Cremers, Bas Van Genabeek, Joost H.C.M. Kreijtz, Esther Lutgens, Niels P. Riksen, Henk M. Janssen, Serge H.M. Söntjens, Freek J.M. Hoeben

Physiology Faculty Publications

Infections, cancer, and trauma can cause life-threatening hyperinflammation. In the present study, using single-cell RNA sequencing of circulating immune cells, we found that the mammalian target of rapamycin (mTOR) pathway plays a critical role in myeloid cell regulation in COVID-19 patients. Previously, we developed an mTOR-inhibiting nanobiologic (mTORi-nanobiologic) that efficiently targets myeloid cells and their progenitors in the bone marrow. In vitro, we demonstrated that mTORi-nanobiologics potently inhibit infection-associated inflammation in human primary immune cells. Next, we investigated the in vivo effect of mTORi-nanobiologics in mouse models of hyperinflammation and acute respiratory distress syndrome. Using 18F-FDG uptake and flow …

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Grassland For Our World, F Riveros Apr 2025

Grassland For Our World, F Riveros

IGC Proceedings (1977-2023)

The present-day role of grasslands and fodder is reviewed with respect agriculture, animal production and environmental concerns. The world 's grazing lands are a finite resource nnd, overall, are being badly mnnaEed; in most developing countries action is required now, before it is too lnte. They will continue to be a major source of animal feed for the foreseeable future; encroachment from cropping will con

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Human Impact On Terrestrial Ecosytems- What We Know And What We Are Doing About It, H A. Mooney Apr 2025

Human Impact On Terrestrial Ecosytems- What We Know And What We Are Doing About It, H A. Mooney

IGC Proceedings (1977-2023)

At present the impact of humankind on the Barth System is substantial mul will become greater as we continue to add over 80 million people to the global population each year. Collectively the human species utilises over a third of the total fresh water that is available, as well as an equivalent amount of tho total terrestrial productivity. Possil energy is being consumed at a prodigious rnte and fuels, among other things, over a half a billion vehicles. To support the large human population the Earth has been greatly reconfigured with much of the land utilised for grazing and agriculture. …

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Grasslands For Sustainable Ecosystems, Malcolm Hadley Apr 2025

Grasslands For Sustainable Ecosystems, Malcolm Hadley

IGC Proceedings (1977-2023)

Grasslnnds present many advantages for examining issues related to sustainability. Grasslands include planted pastures as well as a variety of natural rangelands in situations ranging from semi-arid to subhumid. Diversity is also reflected in the spectrum of grassland users and uses, for example, nomadic pastoralism, extensive ranches, intensive pastures, wildlife tourism, game hunting. And in terms of sustainability of resource management, grasslands encompass a wide range of contrasting situations and conflicts in resource use. Heavy grazing pressure associated with increasing human populations in certain tropical savannas, the implications of rural depopulation to traditional pasture management in alpine regions, and the …

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