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Articles 1981 - 2010 of 52414

Full-Text Articles in Entire DC Network

Apoe4 Reshapes The Lipid Droplet Proteome And Modulates Microglial Inflammatory Responses, Cassi M. Friday, Isaiah O. Stephens, Cathryn T. Smith, Sangderk Lee, Diksha Satish, Nicholas A. Devanney, Sarah Cohen, Josh M. Morganti, Scott M. Gordon, Lance A. Johnson Aug 2025

Apoe4 Reshapes The Lipid Droplet Proteome And Modulates Microglial Inflammatory Responses, Cassi M. Friday, Isaiah O. Stephens, Cathryn T. Smith, Sangderk Lee, Diksha Satish, Nicholas A. Devanney, Sarah Cohen, Josh M. Morganti, Scott M. Gordon, Lance A. Johnson

Physiology Faculty Publications

Excess lipid droplet (LD) accumulation is implicated in various diseases, including Alzheimer's disease (AD), yet the mechanisms underlying this accumulation remain unclear. Apolipoprotein E (ApoE) is a droplet-associated protein, and its E4 variant confers the greatest genetic risk for late-onset AD while also being linked to increased neuroinflammation and LD accumulation. In this study, we compared the lipid and protein composition of hepatic LDs in targeted replacement mice expressing human E3 (neutral) or E4 (risk variant), under both baseline conditions and following lipopolysaccharide (LPS) administration. Lipidomic analysis revealed that E4 LDs exhibit a shift in glycerophospholipid distribution, with an increase …


Making Sense Of Myc In Skeletal Muscle, Ronald G. Jones, Sebastian Edman, Nathan Serrano, Yuan Wen, John J. Mccarthy, Christopher S. Fry, Ferdinand Von Walden, Kevin A. Murach Aug 2025

Making Sense Of Myc In Skeletal Muscle, Ronald G. Jones, Sebastian Edman, Nathan Serrano, Yuan Wen, John J. Mccarthy, Christopher S. Fry, Ferdinand Von Walden, Kevin A. Murach

Physiology Faculty Publications

No abstract provided.


Microrna-1 Regulates Metabolic Flexibility By Programming Adult Skeletal Muscle Pyruvate Metabolism, Ahmed Ismaeel, Bailey D. Peck, Mclane M. Montgomery, Benjamin I. Burke, Jensen Goh, Abigail B. Franco, Qin Xia, Katarzyna Goljanek-Whysall, Brian Mcdonagh, Jared M. Mclendon, Pieter J. Koopmans, Daniel Jacko, Kirill Schaaf, Wilhelm Bloch, Sebastian Gehlert, Kevin A. Murach, Kelsey H. Fisher–Wellman, Ryan L. Boudreau, Yuan Wen, John J. Mccarthy Aug 2025

Microrna-1 Regulates Metabolic Flexibility By Programming Adult Skeletal Muscle Pyruvate Metabolism, Ahmed Ismaeel, Bailey D. Peck, Mclane M. Montgomery, Benjamin I. Burke, Jensen Goh, Abigail B. Franco, Qin Xia, Katarzyna Goljanek-Whysall, Brian Mcdonagh, Jared M. Mclendon, Pieter J. Koopmans, Daniel Jacko, Kirill Schaaf, Wilhelm Bloch, Sebastian Gehlert, Kevin A. Murach, Kelsey H. Fisher–Wellman, Ryan L. Boudreau, Yuan Wen, John J. Mccarthy

Physiology Faculty Publications

Objective: Metabolic flexibility refers to the ability of tissues to adjust cellular fuel choice in response to conditional changes in metabolic demand and activity. A loss of metabolic flexibility is a defining feature of various diseases and cellular dysfunction. This study investigated the role of microRNA-1 (miR-1), the most abundant microRNA in skeletal muscle, in maintaining whole-body metabolic flexibility. Methods: We used an inducible, skeletal muscle-specific knockout (KO) mouse model to examine miR-1 function. Argonaute 2 enhanced crosslinking and immunoprecipitation sequencing (AGO2 eCLIP-seq) and RNA-seq analyses identified miR-1 target genes. Metabolism was investigated using metabolomics, proteomics, and comprehensive bioenergetic and …


Transferrin Receptor 1 Deficiency In Smooth Muscle Cells Attenuates Vascular Remodeling In Hypertensive Mice, Yoshiro Naito, Hisashi Sawada, Tetsuo Horimatsu, Masaki Ohmuraya, Masanori Asakura, Masaharu Ishihara Aug 2025

Transferrin Receptor 1 Deficiency In Smooth Muscle Cells Attenuates Vascular Remodeling In Hypertensive Mice, Yoshiro Naito, Hisashi Sawada, Tetsuo Horimatsu, Masaki Ohmuraya, Masanori Asakura, Masaharu Ishihara

Physiology Faculty Publications

Transferrin receptor 1 (TfR1), a crucial cellular iron receptor, has a variety of biological functions. We have previously reported that TfR1 abundance increases in the aortic media of hypertensive rat models. However, its role in vascular diseases remains unknown. In the present study, we generated smooth muscle cell (SMC)-specific TfR1 deficient mice and examined the impact of its deletion in mouse models of hypertension induced by angiotensin II or deoxycorticosterone acetate/salt administration. SMC-specific TfR1 deletion attenuated medial thickening and elastin fragmentation in both mouse models of hypertension. These results indicate that TfR1 in SMCs exerts a role in vascular remodeling …


Synthesis Of Novel Derivatives From Machilin C/D As Antiproliferative Agents Against Triple Negative Breast Cancer, Alyson M. Ackerman, Chibuzor Olewele, Bert C. Lynn, Samuel G. Awuah Aug 2025

Synthesis Of Novel Derivatives From Machilin C/D As Antiproliferative Agents Against Triple Negative Breast Cancer, Alyson M. Ackerman, Chibuzor Olewele, Bert C. Lynn, Samuel G. Awuah

Chemistry Faculty Publications

Lignans are small polyphenolic compounds that play an active role in plant defense against pathogens and predators. Recently, lignan machilin D was reported to be effective as an anti-tumorigenic agent in triple negative breast cancer (TNBC) tumor-bearing mice. Previous studies have relied on plant-extracted material limiting scalability and diversification of the natural scaffold. Herein, we describe a generalizable one-pot synthesis of machilin D and its derivatives via an iron chloride-induced dimerization of isoeugenol. Employing this synthetic methodology allowed for a robust diversification campaign to access seven (7) new lignan derivatives of the machilin D family with superior anti-proliferative properties in …


Proto-Oncogene Hras Transcript Level And Overall Survival In Stages Ii And Iii Colorectal Cancer, Donghyun Kim, Saima Sharif, Juan Antonio Raygoza Garay, Avanish S. Bhakta, Patrick M. Boland, Michael J. Cavnar, Michelle L. Churchman, Hassan Hatoum, Lyen C. Huang, Joseph Kim, Richard Kim, Robert W. Lentz, Sarbajit Mukherjee, Mary T. O'Donnell, Benjamin Quartey, Matthew J. Reilley, Robert J. Rounbehler, Bodour Salhia, Bryan P. Schneider, Carlos H. Chan Aug 2025

Proto-Oncogene Hras Transcript Level And Overall Survival In Stages Ii And Iii Colorectal Cancer, Donghyun Kim, Saima Sharif, Juan Antonio Raygoza Garay, Avanish S. Bhakta, Patrick M. Boland, Michael J. Cavnar, Michelle L. Churchman, Hassan Hatoum, Lyen C. Huang, Joseph Kim, Richard Kim, Robert W. Lentz, Sarbajit Mukherjee, Mary T. O'Donnell, Benjamin Quartey, Matthew J. Reilley, Robert J. Rounbehler, Bodour Salhia, Bryan P. Schneider, Carlos H. Chan

Surgery Faculty Publications

Background: Mutational landscape is prognostic in colorectal cancer (CRC). Rat sarcoma (RAS) oncogenes, such as KRAS and NRAS, with driver mutations, portend poor survival outcomes, whereas pathologic mutations in HRAS are extremely rare, and their prognostic value remains uncertain. Methods: This retrospective study analyzed the Oncology Research Information Exchange Network (ORIEN) alliance tumor RNA-Seq data in Stages II and III CRC to investigate the association between RAS gene expression and survival outcomes. Results: High transcript levels of HRAS were associated with superior overall survival (OS). The high HRAS-associated OS benefit was most pronounced in patients with right-sided primary expressing low …


Β-Aminopropionitrile-Induced Thoracic Aortopathy Is Refractory To Cilostazol And Sildenafil In Mice, Samuel C. Tyagi, Sohei Ito, Jacob C. Hubbuch, Michael K. Franklin, Deborah A. Howatt, Hong S. Lu, Alan Daugherty, Hisashi Sawada Aug 2025

Β-Aminopropionitrile-Induced Thoracic Aortopathy Is Refractory To Cilostazol And Sildenafil In Mice, Samuel C. Tyagi, Sohei Ito, Jacob C. Hubbuch, Michael K. Franklin, Deborah A. Howatt, Hong S. Lu, Alan Daugherty, Hisashi Sawada

Surgery Faculty Publications

Thoracic aortopathies are life-threatening diseases including aneurysm, dissection, and rupture. Cilostazol, a phosphodiesterase (PDE) 3 inhibitor, and sildenafil, a PDE5 inhibitor, have been used clinically for peripheral arterial disease and erectile dysfunction or pulmonary hypertension, respectively. Recent studies report their effects on abdominal aortic aneurysm formation. However, their impacts on thoracic aortopathy remain unknown. In this study, we investigated whether cilostazol and sildenafil affect thoracic aortopathy induced by β-aminopropionitrile (BAPN) administration in mice. Bulk RNA sequencing analysis revealed that BAPN administration upregulated Pde3a transcription in the ascending aorta and Pde5a in both ascending and descending regions before thoracic aortopathy formation. …


Predictors Of Permanent And Temporary Motor Deficits In Patients Undergoing Glioma Resection, Brian J. Conway, Stephanie A. Armstrong, Nada Botros, Sergey Tarima, Max O. Krucoff Aug 2025

Predictors Of Permanent And Temporary Motor Deficits In Patients Undergoing Glioma Resection, Brian J. Conway, Stephanie A. Armstrong, Nada Botros, Sergey Tarima, Max O. Krucoff

Biostatistics Faculty Publications

Objective: To identify predictors of permanent and temporary motor deficits in patients undergoing glioma resection. Methods: A literature search accessed the databases Ovid Medline, Scopus, Web of Science, Cumulative Index to Nursing and Allied Health Literature/EBSCO, PsychInfo, Cochrane, and Wiley for studies reporting motor outcomes following surgical resection of glioma. Outcomes were stratified by patient/tumor characteristics, preoperative condition, and intraoperative factors for both permanent and temporary motor deficits. Generalized estimating equations were used to generate odds ratios. Results: A total of 1332 titles and abstracts were reviewed resulting in the evaluation of 261 full-text articles. Data were extracted from 67 …


The Effect Of Cellulolytic Enzyme Preparations As In Vitro Improvement For Forage Digestibility, G Bertin, Ph Hellings Jul 2025

The Effect Of Cellulolytic Enzyme Preparations As In Vitro Improvement For Forage Digestibility, G Bertin, Ph Hellings

IGC Proceedings (1977-2023)

The systematic study of commercial preparations of fungal cellulases has been undertaken with the aim of determining the principal components and the contribution of each to the digestion of the plant cell wall. These enzymes, normally have three fold role-silage preservative by hydrolysing the cellulosic wall of plant material, cellulases make available fermentable sugars to the lactic bacteria. -Improvement of digestibility we can increase gain by 1 % of the dry matter in forage. - Improvement of intake if the animal digests more quickly his gut will empty more rapidly thus making it available sooner for a new intake of …


Effect Of Cellulase Preparation On Fermentation Of Silage, E No, Y Harasawa, A Ataku, N Narasaki, T Sueyoshi Jul 2025

Effect Of Cellulase Preparation On Fermentation Of Silage, E No, Y Harasawa, A Ataku, N Narasaki, T Sueyoshi

IGC Proceedings (1977-2023)

The effects of cellulase preparation (Meicelase) on silage fermentation were studied using the first cutting alfalfa and timothy. In one-liter laboratory silos with Meicelase of 0, 0.2, 0.4 and 0:8% of the fresh grasses, the fermentative quality was examined throughout a 30 day ensilage period. The amount of the lactic acid in the silage with the Meicelase was considerably greater than the control silage with no additive. In addition, the pH of that silage was low and the content of water soluble carbohydrate (WSC) was high. However, the differences in the amount of Meicelase applied had no effects in the …


Effects Of Sample Drying Method On Estimates Of Nutritive Value Value, S Uchida, H Hayashi, T Horigome Jul 2025

Effects Of Sample Drying Method On Estimates Of Nutritive Value Value, S Uchida, H Hayashi, T Horigome

IGC Proceedings (1977-2023)

The influence of different drying methods for the sample used for analysis on the estimates of the nutritive value of silage was investigated. The silages and hay were made from the same grass and fed to three wethers to determine digestibilities. The samples for analytical use of silages and hay fed, and feces excreted, were prepared by three drying techniques of freeze drying (F), oven drying at 60 C (L) and oven drying at 100 C (H) and then each dried sample was ground in a Wiley mill to pass a 0.5 mm screen. The residual moisture in prepared samples …


Effect Of Lactic Acid Bacteria On Silage Fermentation, Ph Hellings, G Bertin, M Vanbelle Jul 2025

Effect Of Lactic Acid Bacteria On Silage Fermentation, Ph Hellings, G Bertin, M Vanbelle

IGC Proceedings (1977-2023)

Inoculants were tested to determine their suitability for use as silage aids. Studies carried out in laboratory indicated (1) that the minimum inoculum should be at least 105 viable organisms per gram of fresh fodder and (2) of all bacterial strains tested, only the Lactobacillus and, specifically, L. casei improved storage characteristics. But the efficiency of an inoculant was subordinated to the fermentable sugars content of the crops. In this respect, alfalfa and second cut grass had the lowest potential for ensilage. Energy was supplied as a mixture of milled barley and amylolytic complex. The influence of a 1: 1: …


Ensilage Of Alfalfa Press-Cake. A Kinetic Model For Ph, Acidity And Nitrogen Evolution, G Amdrich, L Lencioni, R Florentini, G Anelli Jul 2025

Ensilage Of Alfalfa Press-Cake. A Kinetic Model For Ph, Acidity And Nitrogen Evolution, G Amdrich, L Lencioni, R Florentini, G Anelli

IGC Proceedings (1977-2023)

A kinetic model to describe the time evolution of pH, acidity, metabolizable nitrogen and k1 • B, ( a function related to the bacteria concentration) during ensilage of alfalfa press-cake is suggested. The basic hypotheses of the model are given together with the related differential and integral equations. The kinetic model has been tested using experimental data obtained from two samples of alfalfa press-cake respectively ensiled with and without acid addition. The correlation coefficients obtained indicate the reliability of the suggested model.


Ensilage Of Alfalfa Press-Cake, G Anelli, G Andrich, M Contini, L Lencioni Jul 2025

Ensilage Of Alfalfa Press-Cake, G Anelli, G Andrich, M Contini, L Lencioni

IGC Proceedings (1977-2023)

The Istituto Industrie Agrarie of the University of Pisa has developed a wet green crop fractionation process which involves the ensilage of the press-cake. This note reports some experimental results on the alfalfa press-cake ensiled with or without addition of acid. The evolution of silages was evaluated through organoleptic and chemical parameters. The good behaviour· during the ensilage shows that alfalfa press-cake is suitable to be ensiled even without previous acidification.


Coagulation Of Sheep Milk Added With Alfalfa Juice And Protein Concentrate, L Lencioni, R Florentini, A M. Pisanelli, C Galoppini Jul 2025

Coagulation Of Sheep Milk Added With Alfalfa Juice And Protein Concentrate, L Lencioni, R Florentini, A M. Pisanelli, C Galoppini

IGC Proceedings (1977-2023)

This note reports some experimental results on the preparation of food-grade products obtained by enzyme coagulation of sheep milk added with alfalfa juice, decolourized whole protein concentrate and cytoplasmic protein concentrate. Working conditions are reported together with the yields, chemical composition and organoleptic properties of the coagula. Products obtained from milk supplemented with low alfalfa juice (��5% dry basis) or protein concentrates (;;;;:10% dry basis) show a good acceptability. The ripening greatly reduces grass flavour and taste.


Effect Of Remaining Brown Juice In Wet Protein Curd On The Quality Of Leaf Protein After Drying, Y S. Cho, J K. Kim, T Horigome, S Uchifa Jul 2025

Effect Of Remaining Brown Juice In Wet Protein Curd On The Quality Of Leaf Protein After Drying, Y S. Cho, J K. Kim, T Horigome, S Uchifa

IGC Proceedings (1977-2023)

The influence of remaining brown juice ( deproteinised juice) in wet protein curd on the quality of resulting leaf protein was examined. Freshly cut Italian ryegrass and red clover were fractionated into protein curd, brown juice and pressed cake. Various leaf proteins were prepared by drying the protein curds retaining different amounts of brown juice at 65°C followed by acetone-washing, and by immediate acetone-washing of the protein curd. Casein-brown juice mixtures at the ratio of 1 :3 and 1: 6 were also prepared. The crude protein content of the dry matter in brown juice was very low, 9. 9% for …


The Nutritive Value Of Mixtures Of White Leaf Protein And Other Food Proteins, R Carlsson, P Hanczakowski Jul 2025

The Nutritive Value Of Mixtures Of White Leaf Protein And Other Food Proteins, R Carlsson, P Hanczakowski

IGC Proceedings (1977-2023)

The nutritive value of white leaf proteins were studied, as food supplements. The protein was obtained by heat­fractionation ( Chenopodium quinoa Willd.) or by de-pigmentation (Medicago sativa L. ). The leaf protein was substituting 50% of the protein in a diet, where the remaining 50% came from wheat gluten, soya bean meal, bone-meat meal, fish meal, skim milk or whey. The nutritive value was estimated by the Thomas-Mitchell nitrogen balance method. The proximate chemical composition of the food protein products, and their amino acid composition were analysed. In every mixture but the one with gluten the biological value was higher …


Effect Of Contamination By Aspergillus Flavus On The Nutritive Value Of Leaf Protein Concentrate Of Leucaena Leucocphala During Storage, H T. Ostrowski-Meissner, I Komang, W Supratman, I Barchia, S Cacang Jul 2025

Effect Of Contamination By Aspergillus Flavus On The Nutritive Value Of Leaf Protein Concentrate Of Leucaena Leucocphala During Storage, H T. Ostrowski-Meissner, I Komang, W Supratman, I Barchia, S Cacang

IGC Proceedings (1977-2023)

Leaf protein concentrate extracted from tree legume Leucaena leucocephala cultivar Cunningham (LLP) was stored under humid tropical conditions (24-28°C and 84-92% rh) in the form of wet cake during a 10 day period. Samples of LLP were taken every second day for chemical analyses and protein quality assays. The effect of storage on dried LLP was also assessed against two control proteins: peanut meal (PNM) and fish meal (FM), either free of aflatoxins or infected by the aflatoxigenic fungus Aspergi_llus flavus to the level 280 mcg aflatoxin B1 equivalent/kg. Storage of wet LLP under such conditions resulted in infestation by …


Physiological Activity Of Alfalfa Lpc And Brown Juice, A Sagasaki, T Kawakami, M Naito, H Yamada, Y Kuroyanagi, K Suzumura, R Kayama Jul 2025

Physiological Activity Of Alfalfa Lpc And Brown Juice, A Sagasaki, T Kawakami, M Naito, H Yamada, Y Kuroyanagi, K Suzumura, R Kayama

IGC Proceedings (1977-2023)

With the purpose of searching physiological active component(s) contained in alfalfa leaf, green leaf protein concentrate (LPC) and brown juice were tested against the effects on the inutagenicity of mutagen and hyperlipemia. LPC and brown juice were found to inactivate the mutagenicity of 2•aminoanthracene by the Ames test, and the capacity was more powerful in LPC than in brown juice. Further feeding of alfalfa LPC or its brown juice added diet to hamster reduced the total cholesterol and triglyceride levels in serum.


Influence Of The Age Of Chicken On The Metabolizable Energy Value Of Alfalfa Leaf Protein Concentrate, S Terapuntuwat, I Tasaki Jul 2025

Influence Of The Age Of Chicken On The Metabolizable Energy Value Of Alfalfa Leaf Protein Concentrate, S Terapuntuwat, I Tasaki

IGC Proceedings (1977-2023)

Influence of the age of chicken on the nitrogen corrected metabolizable energy (MEn) value of alfalfa leaf protein concentrate (LPC) was investigated. Chickens of various ages were fed diets containing certain amounts of the LPC, and droppings were collected quantitatively. The MEn of the diets was determined as the difference between consumed energy and excreted energy which was corrected with retained energy as body protein. Then the MEn value of the LPC was calculated using the values of the LPC containing diets and that of cornstarch which was obtained from data for a LPC-free diet. As a result, the MEn …


High Value Productions From Lucerne: A New Zealand Perspective, R M. Mcdonald, P E. Donnelly, R A. Mills, S R. Vaughan Jul 2025

High Value Productions From Lucerne: A New Zealand Perspective, R M. Mcdonald, P E. Donnelly, R A. Mills, S R. Vaughan

IGC Proceedings (1977-2023)

Research at Ruakura has aimed at improving the economics of lucerne protein extraction, by extracting more of the protein (up to 85%) and by searching for ways to get maximum value for each of the components in the lucerne. Presently most lucerne protein concentrate is sold on the basis of its protein and xanthophyll contents, which are valued by poultry growers. Other components, including carotene, chlorophyll and vitamin E, are not valued in poultry feed and consequently could be extracted and sold separately. Carotene and vitamin E have recently been associated with cancer prevention. The paper also discusses the value …


Effect Of Drying Methods On The Nutritive Value Of Leaf Protein Concentrates Coagulated By Acidification, Heat Or Anaerobic Fermentation, M Ohshima Jul 2025

Effect Of Drying Methods On The Nutritive Value Of Leaf Protein Concentrates Coagulated By Acidification, Heat Or Anaerobic Fermentation, M Ohshima

IGC Proceedings (1977-2023)

SUMMARY To determine the effect of heat-treatments on the nutritive value of leaf protein concentrate(LPC), several LPCs prepared by different procedures were fed to rats. The nutritive value of ladino clover LPC separated centrifugally from acidified juice and freeze-dried was compared with that of LPC prepared from the same acidified juice by heating to 70°C with an electric heater before the centrifugation and oven-dried at 70°C. The heat-treatments reduced not only the apparent digestibility of crude protein by 5%, but also the nutritionally available lysine content of LPC. Response of rats to lysine supplementation in addition to methionine was observed …


Effect Of Anaerobic Fermentation Of Plant Juice On The Yield And The Nutritive Value Of Leaf Protein Concentration, M Ohshima, H Ueda Jul 2025

Effect Of Anaerobic Fermentation Of Plant Juice On The Yield And The Nutritive Value Of Leaf Protein Concentration, M Ohshima, H Ueda

IGC Proceedings (1977-2023)

Effect of treatments of plant juice on the yield and nutritive value of leaf protein concentrate (LPC) was studied with special reference to anaerobic fermentation using lucerne and Italian rye grass as the materials. Lucerne juice was .subdivided into three portions. The two portions were heated to 70°C immediately or after being adjusted to pH 8 with NaOH. The other portion was anaerobically fermented for 7 days. One half of ryegrass juice was heated to 70°C after being adjusted to pH 4 with HCI and the other half was submitted to the fermentation. After the treatments, each juice was centrifuged …


Alkali Treatment Of Rice Whole Crop Silage, T Tobino, N Mikami, A Yamazaki, R Kobayashi, K Furugouri Jul 2025

Alkali Treatment Of Rice Whole Crop Silage, T Tobino, N Mikami, A Yamazaki, R Kobayashi, K Furugouri

IGC Proceedings (1977-2023)

Application of Na OH for rice whole crop at the time of ensiling increased digestibility, intake and body weight gain of Holstein steers. As rice whole crop silage is inclined to cause secondary fermentation, when the moisture content is not sufficient, water addition to the filling material is necessary. The suitable application level of NaOH is 3 to 4% of silage dry matter and it can be applied successfully using 20 to 30% solution. The rate of notreacted Na in the NaOH applied silages was 20 to 40% and it changed wholly to the form of Na2CO3 -Na. The greater …


The Effect Of The Harvest Time, Height And Length Of Cutting On The Quality And Nutrient Yield Of Japanese Millet Silage, T Nakui, N Yahara, S Takai Jul 2025

The Effect Of The Harvest Time, Height And Length Of Cutting On The Quality And Nutrient Yield Of Japanese Millet Silage, T Nakui, N Yahara, S Takai

IGC Proceedings (1977-2023)

The experiments were made to determine the appropriate harvest time, height and length of cutting of Japanese millet for silage. Harvesting the millet at the end of the late dough stage was found to be the most favorable for silage, and the fermenting quality of the silage was improved by higher cutting and keeping the length of the cutting short ( < 10 mm). Dry weight yield of the plant (x) well correlated with TDN yield (Y) of the silage (r = 0.91, Y = 0.511x - 64.7). A negative correlation was obtained between the rate of temperature rise during the second fermentation process of silage broken the seal and the content of butyric acid in the silage. The second fermentation was suppressed when the butyric acid content in the total acid of the silage was more than 3 % .


Effect Of Stage Growth And Chopping Length On Ensiling Characteristics, Digestibility And Palatability Of Guinea-'A', S Panditharatne, J P. Fontenot, V G. Allen, M.C N. Jayasuriya Jul 2025

Effect Of Stage Growth And Chopping Length On Ensiling Characteristics, Digestibility And Palatability Of Guinea-'A', S Panditharatne, J P. Fontenot, V G. Allen, M.C N. Jayasuriya

IGC Proceedings (1977-2023)

Ensiling of forages is a common practice in temperate areas, but is not used extensively in tropical countries. Preservation of excess forage during rainy seasons would provide a more even distribution of feed during the year. Experiments were conducted to investigate fermentation characteristics, and digestibility and palatability of Guinea-'A' (Panicum maximum, ecotype A) silage by sheep. Guinea-'A' grass was harvested after 2- and 3-week growth periods, and ensiled chopped or unchopped in 210 liter metal drums, double lined with polyethylene. Cutting grass at 2 weeks decreased pH and increased lactic acid of silage, compared to cutting at 3 weeks. Chopping …


Analysis Of The Factors Affecting On Disappearance Of Nitrate In Grass During Ensilage, T Masuko, T Otani, K Awaya Jul 2025

Analysis Of The Factors Affecting On Disappearance Of Nitrate In Grass During Ensilage, T Masuko, T Otani, K Awaya

IGC Proceedings (1977-2023)

From 1979 to 1985, a series of experiments was carried out. In these experiments, Italian rye grass containing large amounts of nitrate was ensiled in laboratory silos with CaCO3, glucose and urea immediately after harvesting, and at three moisture levels adjusted by pre-wilting. In each experiment, the grasses were chopped to about 2 cm length and then packed in 1.5 liter glass bottles. The silos were kept at 28°C in the incubator and opened 50-80 days after ensiling. The nitrate content and crude protein content of original grass were-high and WSC content of that was low. In thirty grass silages …


A Technique To Study The Relative Merits Of Different Silage Additves, A D. Drysdale, D A. Parker Jul 2025

A Technique To Study The Relative Merits Of Different Silage Additves, A D. Drysdale, D A. Parker

IGC Proceedings (1977-2023)

Expansion in the use of silage additives in the more difficult silage-making areas of Europe has created the need for a simple laboratory method to assess the relative values of the wide range of products now offered. A technique is described which can be used to study potential new additives and to monitor existing products. Using direct-cut lucerne(Medicago sativa) ensiled quickly it is possible to produce a high pH, poorly fermented, untreated silage and against this the relative effects of different additive treatments can be assessed. In an example discussed, lucerne of 14% dry matter (DM) and buffering capacity 44m-equiv …


Control Of Silage Fermentation By Inoculation With Lactobacillus Casei, K Ataku, H Nakamura, N Narasaji, M Kikuchi Jul 2025

Control Of Silage Fermentation By Inoculation With Lactobacillus Casei, K Ataku, H Nakamura, N Narasaji, M Kikuchi

IGC Proceedings (1977-2023)

The D( -) isomer of lactic acid is largely produced by silage fermentation. Accordingly, this study focused on Lactobacillus casei (LC) in which produces L( +) lactic acid. The influences of the inoculation of LC and Lactobacillus plantarum (LP) on the quality of silage and on the isomer ratio of the lactic acid were examined with and without 2% glucose addition at two ensilage temperature (20°C and 30°C). Orchardgrass and alfalfa were ensiled in laboratory silos, capacity one-liter. When either LP and LC was added, a large quantity of lactic acid was produced, the pH dropped sharply, and the quality …


Losses During Ensiling Due To Gas Exchange, H Honig, E Zimmer Jul 2025

Losses During Ensiling Due To Gas Exchange, H Honig, E Zimmer

IGC Proceedings (1977-2023)

Gas exchange leading to air infusion into the silo during the storage period is one of the main reasons for poor silage quality and additional losses. To make these losses better predictable, and to show ways to ensiling technology to avoid them, the parameters influencing the gas exchange in silage stacks have to be defined and quantified. The motive powers for this gas exchange are "breathing" in gastight systems, and CO2 flow off in "open" systems. Recent experiments using 6 m3 pilot silos to evaluate and quantify these losses, especially those due to CO2 flow off, show the following results: …