Digital Commons Network^™

C/Ebpα Is Essential For Gonadal But Not Inguinal White Adipose Tissue Formation In Mice, Krista Y. Hu, Yu-Lin Ma, Esme A. Dodge, Olivia A. B. Maguire, Caio V. Matias, Ryan K. Barney, Hector S. Himede, Juliana Gomez Pardo, Miriam Cepeda, Scott M. Gordon, Robert C. Bauer

Physiology Faculty Publications

Objective: The distribution of excess white adipose tissue (WAT) in obesity correlates with risk for comorbidities. Thus, understanding depot-specific WAT developmental mechanisms is translationally relevant. SNPs near the gene CEBPA associate with waist to hip ratio, and while C/EBPα is a recognized regulator of adipogenesis, there is no previously known role for C/EBPα in regulating adipose distribution.

Methods: We crossed Cebpa floxed mice to the AdipoQ-Cre transgenic mouse strain, generating mice with adipocyte-specific knockout of Cebpa (Cebpa_ASKO). Mice were phenotyped on a chow diet and after prolonged high-fat diet (HFD) feeding.

Results: Cebpa_ASKO mice almost entirely lack gonadal …

Dim Light At Night Worsens Cardiac Autonomic Dysregulation In Female Diabetic Mice, Abhilash Prabhat, Shrishti Naidu, Isabel G. Stumpf, Tanya S. Seward, Elizabeth A. Schroder, Brian P. Delisle

Physiology Faculty Publications

Cardiovascular autonomic neuropathy (CAN) is a common complication of diabetes mellitus. CAN is characterized by impaired cardiovascular autonomic control and is associated with increased mortality, especially among women with type 2 diabetes. Identifying environmental factors that exacerbate the CAN-associated phenotype may reveal modifiable strategies to reduce cardiovascular risk.

Nighttime light exposure is a novel cardiovascular risk factor. Among ∼90,000 individuals, greater nighttime light exposure correlated with increased atrial fibrillation and heart failure risk. Light at night disrupts sympathovagal balance and elevates nocturnal heart rate in humans. Dim light at night similarly disrupts autonomic regulation of heart rate in mice. We …

Early Microglial Response To Amyloid Plaques Drives Sleep Loss In Alzheimer’S Disease, Nicholas J. Constantino, Riley E. Irmen, Matthew J. Lanning, Sierra M. Turner, Clair C. Ashley, Caitlin M. Carroll, Evan M. Neary, Dave Rubinow, J. Andy Snipes, Shannon L. Macauley

Physiology Faculty Publications

Introduction: Sleep disruption is an early feature of Alzheimer's disease (AD), but the cellular mechanisms linking amyloid pathology to sleep loss remain unclear.

Methods: Electroencephalography/electromyography (EEG/EMG) recordings, quantitative EEG analysis, and sleep deprivation were performed in APPswe/PSEN1dE9 (APP/PS1) mice at different stages of pathology relative to normal aging. Amyloid burden and microglial density were quantified with whole-brain light-sheet microscopy. CSF1R-mediated microglial depletion explored effects of microglia on sleep loss.

Results: Amyloid plaques caused non–rapid eye movement (NREM) sleep loss that did not worsen with increased plaque burden. Aging reduced REM sleep and eliminated sleep rebound. Amyloid pathology was associated with …

Dennd5b Disruption Results In Reduced Body Fat And Increased Intestinal Fatty Acid Oxidation By Activation Of Autophagy, Khaga R. Neupane, Alexander Karakashian, Maura Mobilia, Olivia Hage, Patrick Tso, Min Liu, Laurent Vergnes, Karen Reue, Scott M. Gordon

Physiology Faculty Publications

Intestinal absorption of dietary lipid is essential for systemic lipid homeostasis; however, elevated postprandial plasma lipid levels are associated with obesity and increased risk for atherosclerotic cardiovascular disease. In humans and rodents, biological sex impacts dietary triglyceride absorption, and males tend to have higher postprandial triglyceride levels compared to females, but the physiological basis for this is not well understood. Here, we show that the gene DENND5B is associated with body composition in humans and mice and that genetic deletion of Dennd5b in mice prevents postprandial plasma triglyceride elevations in both sexes. Our findings establish a role for this protein …

Evidence Of Interparticle Chylomicron “Bridging” In Mouse Mesenteric Lymph After A Lipid Bolus, Khaga Raj Neupane, Alexander Karakashian, Min Liu, Scott M. Gordon

Physiology Faculty Publications

No abstract provided.

Pediatric Chop Chemotherapy Acutely Disrupts Satellite-Cell Dynamics And Blunts Muscle Mass In A Sex-Specific Manner, Jainil Daredia, Marc A. Magaña, Carla M.C. Nascimento, Jaden M. Wells, Nicholas T. Thomas, Yuan Wen, Savannah V. Rauschendorfer, Cory M. Dungan, Michael P. Wiggs

Physiology Faculty Publications

Pediatric cancer survival now exceeds 85% owing, in part, to advances and the use of combination chemotherapy treatments such as CHOP (cyclophosphamide, doxorubicin, vincristine, and prednisone). Despite its efficacy, CHOP may cause off-target effects during critical pediatric development periods such as impairments of skeletal muscle. We evaluated the acute effects of a CHOP administered to C57Bl/6J mice from postnatal day 28 to 48. CHOP slowed body-weight gain and led to a smaller gastrocnemius fiber cross-sectional area by ~25% in both sexes (n ¼ 11 or 12 males; n ¼ 8 or 9 females). RNA sequencing detected 214 differentially expressed genes …

Skeletal Muscle Methylome-Transcriptome Disruptions During The Onset And Progression Of Colorectal Cancer-Induced Cachexia, Ana Regina Cabrera, Ronald G. Jones, Eleanor R. Shrems, Francielly Morena, Yuan Wen, Tyrone A. Washington, Kevin A. Murach, Nicholas P. Greene

Physiology Faculty Publications

Cancer cachexia is a wasting condition, primarily affecting skeletal muscle, impairing patients’ quality of life, prognosis, and survival. The molecular triggers are incompletely defined, but given prior evidence for epigenetic plasticity in muscle, we speculate that dysregulated DNA methylation plays a role in muscle transcriptional alterations mediating cachexia severity. We aimed to describe and integrate the cachexia methylome and transcriptome. We used a time course approach in a mild cachexia model (colon-26, C26) coupled with a severe cachexia genetic model (Apc^Min/+) in both biological sexes to assess the methylome across degrees of cachexia pathology. The muscle methylome and …

Cytoprotective Role Of Octacosanol In Lipopolysaccharide-Induced Inflammation, Jingrong Tang, Zhi Hong Yang, Wenling Li, Huaitian Liu, Diego Lucero, Denis Sviridov, Olivia White, Julia Kun, Yoh Suke Mukouyama, Alan T. Remaley

Physiology Faculty Publications

Inflammation and endothelial dysfunction are key steps in the pathogenesis of atherosclerosis. Octacosanol (C₂₈H₅₈O, OCT), a very-long-chain saturated aliphatic alcohol (VLCA) with 28 carbons, is the main component of policosanol, a nutraceutical mixture of VLCAs (C20-C34) extracted from plants. Polycosanol in animal models is known to reduce atherosclerosis, but its mechanism of action remains unclear. This study investigates the pathways by which OCT alleviates LPS-induced inflammation in primary human aortic endothelial cells (HAECs). After overnight pretreatment with purified OCT, inflammation in HAECs was induced by lipopolysaccharide (LPS) at 100 ng/ml. The effects of OCT on the …

From Lipoprotein Metabolism To Blood Clotting, Alexis N. Smith, Mirza A. Beg, Woosuk Steve Hur, David Ginsburg, Iftikhar J. Kullo, Sean S. Davies, Ira Tabas, Jesse W. Williams, Chieko Mineo, Bishuang Cai, Rana K. Gupta, Jaume Amengual, Anna Schwendeman, Gregory A. Graf, Mary Sorci Thomas, Ze Zheng

Physiology Faculty Publications

The biannual Fredrickson Lipid Research conference took place in person in Milwaukee, Wisconsin, from September 3–5, 2025. Each conference highlights the most advanced basic and translational research in lipids and lipoprotein metabolism. As with each Fredrickson Lipid Research conference, the overall theme is focused on “Lipid Metabolism, Lipoproteins, and Atherosclerosis,” and this year included a special scientific session exploring the connections between Blood Clotting and Lipid Metabolism. This session underscored ongoing research aimed at explaining the mechanisms by which dyslipidemia alters the coagulation system and drives thrombotic cardiovascular disease. Other scientific session themes at the conference included Biomarkers in Cardiovascular …

The Impact Of Congenital Heart Disease On The Timing Of Alzheimer's Disease In Down Syndrome, Julianne G. Clina, Brian C. Helsel, Sigan L. Hartley, David A. White, Victoria L. Fleming-Batayneh, Benjamin Handen, Bradley Christian, Elizabeth Head, Mark Mapstone, Christy L. Hom, Beau Ances, Jeffrey Burns, H. Diana Rosas, Florence Lai, Sharon Krinsky Mchale, Joseph H. Lee, Frederick A. Schmitt, Jordan Harp, Ira T. Lott, Shahid Zaman, Lauren T. Ptomey

Neurology Faculty Publications

INTRODUCTION: The incidence of Alzheimer's disease (AD) in Down syndrome (DS) exceeds 90%. Approximately 50% of people with DS have congenital heart disease (CHD). Having CHD increases risk for early-onset AD in populations without DS, but it is unclear if CHD influences AD in DS. METHODS: Data from the Alzheimer Biomarker Consortium-Down Syndrome (ABC-DS) were used. Participants with CHD (n = 82, mean age = 39.9 ± 8.5 years, 97.6% White race) were age- and sex-matched to participants without CHD (n = 82, mean age = 40.5 ± 8.1 years, 98.8% White race). Cognitive assessments and Centiloid load (CL) (positron …

Plasma P-Tau217 And Apoe Genotype, Ann M. Stowe, Bilal Kahn, Ali Ballesteros, Monique Cortez, Linda S. Hynan, Victoria Cantrell, Indu Raman, Guangchun Chen, Eric D. Vidoni, Ellen Binder, Jeffrey Burns, Jeffrey N. Keller, Diana R. Kerwin, Tristyn Hall, Pavel Yanev, Wanpen Vongapatanasin, David C. Zhu, Norman Scheel, C. Munro Cullum, Rong Zhang, Dwight C. German

Neurology Faculty Publications

Introduction: Plasma phosphorylated tau (p-tau)217 levels and apolipoprotein E (APOE) ε4 genotype are used for prodromal identification of individuals at high risk for dementia. We used baseline samples from cognitively normal subjects in the Risk Reduction for Alzheimer's Disease (rrAD) clinical trial (NCT02913664) to investigate the relationship between these two markers in older adults with additional vascular risk factors. Methods: We measured APOE genotype, plasma p-tau217, and standard neuroimaging metrics in 400 rrAD participants, aged 60 to 84 years, with sedentary lifestyle, hypertension, and hypercholesterolemia. Results: Plasma p-tau217 was 57% higher in subjects with at least one APOE ε4 allele, …

Impact Of Covid-19 Positive Status On Outcome For Individuals With Stroke Treated In Acute Inpatient Rehabilitation, Amy Ziems, Christopher J. Mclouth, Nicholas Elwert, Elissa Charbonneau, Joseph Stillo, Susan Mcdowell

Neurology Faculty Publications

Background: Stroke, whether or not accompanied by concurrent COVID-19 infection, has been associated with varying acute care outcomes, with patients who are COVID-19 positive typically faring worse. This study aims to explore the functional outcomes of patients with stroke —with and without simultaneous COVID-19 infection—who survived their acute care stay and progressed to acute inpatient rehabilitation (IRF). Objectives: To identify differences in sociodemographic factors, medical complexity, and rehabilitation outcomes from an IRF stay between patients with stroke with concurrent COVID-19 and those without. Design: A retrospective, observational cohort study using electronic medical records (EMR) data. Setting: EMR data from 138 …

Hyperacute Interleukin-1Β Production And Neutrophil Extracellular Trap Formation In The Cerebral Circulation Of Stroke Patients With Large Vessel Occlusion, Justine Münsterberg, Caspar Brekenfeld, Hanna Englert, Marius Piepke, Regine J. Dress, Martin Oertel, Tina Fischer, Romy Hackbusch, Mingming Zha, Haodi Cai, Christian Casar, Ines S. Schädlich, Leo Winter, Alina Jander, Karoline Degenhardt, Maxim Bester, Fabian Flottmann, Uta Hanning, Brigitte Holst, Anna Worthman, Johanna Hiefner, Berenike Rutz, Eckhard Schlemm, Götz Thomalla, Bettina H. Clausen, Ann M. Stowe, Immo Prinz, Thiruma V. Arumugam, Markus Glatzel, Thomas Renné, Jens Fiehler, Eva Tolosa

Neurology Faculty Publications

Objective: Cerebral ischemia remains a major cause of disability, and the contribution of the hyperacute immune response is increasingly recognized. The aim of this study was to investigate the local inflammatory response in the affected brain of stroke patients with large vessel occlusion during the hyperacute phase of stroke. Methods: To decipher the role of myeloid immune cells in stroke-induced inflammation, we performed an unsupervised multiomics analysis of innate immune pathways in ischemic blood obtained directly from the occluded middle cerebral artery in 54 patients undergoing mechanical recanalization. Paired nonischemic arterial blood served as an internal control. Results: Stroke triggered …

Decoding The Human Pbmc Isonome, Patricia Hayes Doyle, Madeline L. Page, J. Anthony Brandon, Bernardo Aguzzoli Heberle, Brendan J. White, Ann Marie Stowe, Mark T.W. Ebbert

Neurology Faculty Publications

Long-read single-cell RNA sequencing provides an opportunity to understand human health and disease at a level difficult to resolve with bulk or short-read methods. This approach enables isoform-level investigation of cellular diversity and disease mechanisms and definition of cell-types, rather than using genes alone. Using a modified, microfluidic-free PIPseq workflow and computational pipeline adapted for Oxford Nanopore long-read sequencing, we generated the largest long-read single-cell dataset of human peripheral blood mononuclear cells (PBMCs) from a single donor to date, the first with sufficient cell numbers to detect megakaryocytes. This study profiled isoform usage across immune cells, integrating marker expression and …

Interpretable Deep Learning Reveals Spatiotemporal Mri Features Of Brain Aging That Align With Neurodegeneration, Nikhil N. Chaudhari, Owen M. Vega, Phoebe Imms, Jaron M. Kawamura, Nahian F. Chowdhury, Tamara Jafar, Andrei Irimia, Kenneth Spicer, Crystal Flynn Longmire, Jacobo Mintzer, Yaneicy Gonazalez Rojas, V. Sotelo, William Hu, Floyd Jones, Amy Saklad, Sudha Seshadri, Amy Boegel, Sydni Jenee Hill, Paul Newhouse, Rebecca Long, Campbell Long, Arthur Williams, Allison Acree, Olga Brawman-Mintzer, Chelsea Reichert, Vita Pomara, Raymundo Hernando, Nunzio Pomara, Skieff Acothley, Nadeen Elayan, Micah Ellis Slaughter

Neurology Faculty Publications

Cortical thinning and atrophy are hallmarks of brain aging that have been characterized using magnetic resonance imaging (MRI). Brain aging involves many neuroanatomic features whose effects on brain structure remain unexplored. To address this challenge, we trained interpretable deep neural networks (DNNs) to estimate brain age (BA) from T₁-weighted (T₁w) MRI. By identifying MRI features unapparent to humans, DNNs can find aging-related structural alterations above and beyond cortical thickness and atrophy. Using a novel approach to DNN interpretability, we mapped brain aging progression in 25,539 cognitively normal UK Biobank adults aged 45–83 years. Cortical aging is …

Post-Approval Study 3-Year Outcomes Of The Neuroform Atlas Stent For The Treatment Of Wide-Necked Intracranial Aneurysms, Brian Jankowitz, Ashutosh P. Jadhav, Bradley Gross, Tudor G. Jovin, Abdulnasser A. Alhajeri, Justin F. Fraser, Ricardo A. Hanel, Eric Sauvageau, Amin Aghaebrahim, Donald Frei, Richard Bellon, David N. Loy, Ajit S. Puri, Adel M. Malek, Ajith J. Thomas, Gabor Toth, Demetrius Klee Lopes, R. Webster Crowley, John Reavey-Cantwell, Eugene Lin, Adnan Siddiqui, Michael J. Alexander, Ahmad Khaldi, Geoffrey P. Colby, Justin M. Caplan, Sudhakar R. Satti, Aquilla S. Turk, Alejandro M. Spiotta, Richard Klucznik, Danial K. Hallam, David Kung, Michael T. Froehler

Neurology Faculty Publications

Introduction The ATLAS trial was a prospective, multicenter, single-arm, investigational device exemption (IDE) study to evaluate the safety and effectiveness of the Neuroform Atlas Stent System for the treatment of widenecked bifurcation aneurysms. This analysis presents the 36-month follow-up data for the anterior and posterior cohorts. Methods Of the 182patients in the IDE anterior cohort, 146 consented to participate in the post-approval study (PAS) and, of the 116patients in the IDE posterior cohort, 101 consented to participate in the PAS. The primary effectiveness endpoint was core laboratory adjudicated (Raymond–Roy 1; RR1) without retreatment or parent artery stenosis (>50%) at …

Essays In Housing And Consumer Finance, Spencer Stone

Theses and Dissertations--Finance and Quantitative Methods

In my first chapter, I show how consumer left-digit bias affects borrowing costs in the US residential mortgage market. I find that the distribution of mortgage rates bunch at .99-ending rates just below left-digit changing thresholds, and that this bunching is greater in the distribution of rates selected by borrowers than those simply offered by lenders. Comparing observably similar borrowers within the same 1-bp interest rate band, selecting similar mortgage products, in the same census tract and origination month, I show that borrowers receiving .99-ending rates pay significantly more fees than borrowers receiving the nearest whole-number rate just above them, …

Critical Discourse Analysis Of Nil Policies: Examining Institutional Control And Economic Opportunities For Division I Football Athletes In Power Four Universities, Allen T. Alvarez

Theses and Dissertations--Educational Policy Studies and Evaluation

The adoption of Name, Image, and Likeness (NIL) policies has redefined the economic landscape of collegiate athletics, expanding opportunities for student-athletes and reshaping institutional authority. This dissertation nvestigates how NIL policy language at Division I universities frames economic possibilities for football student-athletes and sustains systems of governance. Through a Critical Discourse Analysis of policy documents from Power Four and mid-major institutions, this study reveals that NIL participation is persistently conditioned by institutional approval, compliance monitoring, and ambiguous definitions, especially regarding boosters and collectives.

Policy language presents NIL as an opportunity but embeds institutional control through oversight structures. The analysis further …

The Operationalization Of Student Success: How Institutions And Divisions Of Student Affairs Define And Align Non-Academic Student Success With External Accountability Expectations, Madeline N. Trudeau

Theses and Dissertations--Educational Policy Studies and Evaluation

This qualitative multi-case study investigates how higher education institutions and their divisions of student affairs publicly discuss and define non-academic student success through their strategic planning, within the context of external pressures from state legislatures and accrediting bodies. Grounded in Organizational Learning Theory, Transition Theory, and Theory of Student Involvement and Engagement, this study analyzes the alignment and divergence between external policy expectations and internal institutional and divisional strategic plans.

The findings reveal a fundamental tension; while external stakeholders increasingly demand quantifiable outcomes tied to economic productivity - like post-graduate wages and employability metrics - divisions of student affairs continue …

A Historical Case Study Of The Effects Of The Coordinate Interagency Partnership Regulating International Students (Cipris) System And The Student Exchange Visitor Information System (Sevis) On Foreign Students Coming To The United States For Higher Education From 1990 To 2008, M. Ann Livingstone

Theses and Dissertations--Educational Policy Studies and Evaluation

The United States has welcomed foreign scholars and students since 1774, but their numbers increased dramatically after World War I, and again after World War II, as America became a world power leader and interest in the United States and its educational system and technological developments grew internationally. The Immigration and Naturalization Service (INS) was set up to oversee immigrants, visitors, and nonimmigrants who included students and diplomatic staff and families. This study deals with the changing laws and responsibilities of the INS during the period 1990 to 2008 to track foreign students in higher education, specifically with the introduction …

Climate Change Effects On Biomass And Greenhouse Gas Emissions Are Ameliorated By Nontoxic Endophytes In Southeastern Usa Transition Zone Tall Fescue Pastures, Rebecca Kay Mcgrail, Jonathan D. Moore, A. Elizabeth Carlisle, Jim A. Nelson, Rebecca L. Mcculley

Plant and Soil Sciences Faculty Publications

Background: The plant–microbe interaction between tall fescue and its endophyte Epichloë coenophiala can affect ecosystem responses to changing rainfall patterns and increasing temperatures. Endophyte-infected (E+) stands often exhibit improved stress tolerance and differing soil greenhouse gas (GHG) emissions compared to endophyte-free (E−) stands. However, it is unknown if mammalian nontoxic endophyte (NTE) strains introduced into some tall fescue cultivars confer differing stress tolerances or reduce soil GHG emissions.

Methods: We quantified the impact of fescue-NTE symbioses in Jesup and Texoma on biomass and GHG emissions in response to increased temperature (+3°C) and altered precipitation (50% lengthened interval between events) using …

A Global Synthesis Of Fragipan Silicon Concentrations And Relationships With Soil Physicochemical Properties As A Function Of Soil Parent Materials, Christopher Shepard

Plant and Soil Sciences Faculty Publications

Fragipans are dense, brittle subsurface horizons that restrict root growth and limit water-holding capacity. A common factor in these soils is a binding agent, potentially either phyllosilicate clay bridging, amorphous aluminosilicate/silica, or silica sorbed to Fe oxides. Others have suggested that no binding agent is present within these soil horizons and that the arrangement of individual particles is solely responsible for these horizons. In this article, a secondary data synthesis was utilized to demonstrate that silicon (Si) accumulation is a key pedogenic process in loess-derived fragipan horizons, but not in all fragipans. The average dithionite-extractable Si (Si_d) content …

Impacts Of Rotation, Tillage, Cover Cropping, And Drainage On Soil Health In Soybean-Based Cropping Systems, Tatiane Severo Silva, Lindsay Chamberlain Malone, Matthew D. Ruark, Chad D. Lee, David Jordan, Hanna J. Poffenbarger, Herman J. Kandel, Jeremy Ross, John M. Gaska, Joseph G. Lauer, Laura E. Lindsey, Maninder Pal Singh, Mark A. Licht, Michael Plumblee, Rachel A. Vann, Rodrigo Werle, Spyridon Mourtzinis, Seth L. Naeve, Trenton L. Roberts, Shawn P. Conley

Plant and Soil Sciences Faculty Publications

Recent studies highlight conservation management practices as an effective strategy to enhance soil health. However, results vary, particularly regarding which soil health parameters respond most sensitively to these practices. More studies covering a wide range of soil types and climatic conditions are needed to assist farmers in making management decisions on production practices related to soil health. In this study, we collected soil samples (0–15 cm) from 21 (4–50 years) soybean [Glycine max (L.) Merr.]-based cropping systems trials across the United States (US) to assess the impact of management practices on soil health indicators. Soil indicators included wet aggregate stability …

Autosiq, Liming Zhou, Talukder Z. Jubery, Baskar Ganapathysubramanian, Thomas Lübberstedt, Siddique I. Aboobucker

Plant and Soil Sciences Faculty Publications

Doubled haploid (DH) technology can fast-track crop breeding.Haploid induction yields haploids with only one set of genomes, which are usually sterile.Haploid fertility (HF) is the ability of haploid plants to set seed, and it is a critical bottleneck in DH pipelines.Genetic mechanisms to restore HF hold immense potential in DH crop breeding, yet its phenotyping remains manual, destructive, and inconsistent.While recent advances in imaging and machine learning have improved throughput for general plant traits, no curated image dataset exists for Arabidopsis thaliana that explicitly represents HF.Here, we present AutoSiQ, a dataset and baseline deep learning pipeline for automated HF quantification.AutoSiQ …

Carbon Nanodot Precursors Enable Ultrahigh-Loading Copper Single-Atom Catalysts For Oxygen Reduction Reaction, Prakhar Sharma, Ayanthi Thisera, Matthew G. Boebinger, Jenna K. Rector, Jason M. Unrine, Beth S. Guiton, Doo Young Kim

Plant and Soil Sciences Faculty Publications

Single-atom catalysts (SACs) have attracted significant attention in electrocatalysis due to their high metal utilization and tunable electronic structures; however, their practical implementation is often limited by poor atomic dispersion, low metal loadings (typically < 1 wt%), and insufficient anchoring of single metal atoms on catalyst supports. Herein, we report a simple and versatile strategy to synthesize copper single-atom catalysts (Cu-SACs) with ultrahigh metal loading using citric-acid-derived carbon nanodots as metal-capturing precursors. Through hydrothermal treatment followed by pyrolysis in the presence of urea, atomically dispersed copper atoms coordinated with nitrogen are incorporated into a carbon framework, achieving a Cu loading of up to 20 wt% while maintaining atomic dispersion. Notably, a significant fraction of the copper single atoms exists as Cu⁺–N₂ coordination, which provides under-coordinated and catalytically active sites. The resulting Cu-SAC exhibits promising oxygen reduction reaction (ORR) activity in alkaline media, delivering a limiting current density comparable to that of commercial 20 wt% Pt/C with a predominant four-electron reduction pathway. Despite this high intrinsic activity, durability tests reveal gradual performance degradation under prolonged electrochemical operation, which is attributed to demetallation and aggregation of …

Proposal Of Metagenomic-Origin Lra-5 As A Precursor Of Active Β-Lactamases Through Tyr69gln And Val166glu Amino Acid Substitutions, Gabriela D'Amico González, María Margarita Rodríguez, Pedro Penzotti, Florencia Brunetti, Barbara Ghiglione, Luke A. Moe, Daniela Centrón, Gabriel Gutkind, Lin Gao, Shozeb Haider, Rachel A. Powers, Sebastián Klinke, Pablo Power

Plant and Soil Sciences Faculty Publications

Wild-type LRA-5, recovered from Alaskan soil samples, shares no more than 33% amino acid sequence identity with enzymes from pathogens like PER β-lactamases. Recombinant E. coli expressing wild-type LRA-5 and its engineered variants LRA-5^Y69Q and LRA-5^V166E showed MIC values equivalent to control strains. However, LRA-5^Y69Q/V166E displayed MICs above the resistant breakpoint for some β-lactams. Kinetic parameters correlated with the MICs, showing that the catalytic efficiency of LRA-5^Y69Q/V166E was comparable to those from class A β-lactamases, such as CTX-M-15, PER-2, and KPC-2. LRA-5^Y69Q/V166E exhibited k_cat/K_m values up to 11,000-fold higher compared to wild-type …

Separation Of Trichothecene Mycotoxins In A Matrix Of Hemp (Cannabis Sativa L.) Seed, And Quantification Of Deoxynivalenol In Hemp Grain Infected With Fusarium Graminearum, Isabelle A. Kagan, Magdalena Ricciardi, Henry S. Smith, Rachel R. Schendel, Robert Pearce, Nicole Gauthier

Plant and Soil Sciences Faculty Publications

Background: Fusarium graminearum, one of the causal agents of ear rot in maize and head blight of cereal grains, also infects hemp (Cannabis sativa L. containing a maximum of 0.3% tetrahydrocannabinol or THC). This fungal pathogen produces trichothecene mycotoxins when it infects maize and cereal grains, but little is known about its production of mycotoxins in hemp. A method was developed to separate mycotoxins deoxynivalenol (DON), 15-acetyldeoxynivalenol (15-ADON), and 3-acetyldeoxynivalenol (3-ADON) in commercial hemp seed and grain (harvested tissue), using high-performance liquid chromatography (HPLC) with ultraviolet detection. Results: In HPLC analysis of commercial hemp seed, DON was separated from matrix …

Hybrid Genome And Enzyme Characterization Uncover Novel Glycosyltransferases Associated With Benzylisoquinoline Alkaloid Chemodiversity In Lotus, Hedi Zhao, Xinyi Qi, Yan Liu, Zhuyin Chen, Yifan Wang, Jing Que, Yanyan Su, Ling Yuan, Gangqiang Dong, An Liu, Zhennan Wang, Sha Chen

Plant and Soil Sciences Faculty Publications

Introduction: Hybridization is a key evolutionary force that enriches genetic diversity and is associated with metabolic innovation. Lotus (Nelumbo), comprising Asian lotus (N. nucifera) and American lotus (N. lutea), is both a culturally important ornamental and medicinal plant rich in benzylisoquinoline alkaloids (BIAs). However, the genomic and biochemical basis of BIA glycoside biosynthesis remains poorly understood. Objectives: A high-quality hybrid lotus genome was presented to dissect the formation of benzylisoquinoline alkaloid glycosides and the mechanism underlying their synthesis. Methods: The chromosomal-level hybrid genome was constructed through PacBio HiFi, ONT Ultra-long, and Hi-C sequencing data combined assembly strategies. The metabolite and …

Disrupted Circadian Rhythms Affect Hallmark Pathologies In Alzheimer’S Disease-Related Mouse Models, Valeria Buzinova

Theses and Dissertations--Molecular and Cellular Biochemistry

Alzheimer’s Disease (AD) is a complex neurodegenerative disease with two hallmark pathologies: extracellular amyloid-b (Ab) and intracellular neurofibrillary tangles (NFTs). Ab is proteolytically processed from amyloid precursor protein (APP) by b-secretase and g-secretase as a monomeric peptide prone to aggregation. Eventually, the aggregate-prone monomers will form dense plaques that are difficult to break down and remove. These plaques begin to deposit into the cortex decades prior to the formation of NFTs and the onset of cognitive decline. NFTs are comprised of hyper-phosphorylated tau. Tau is a protein that serves to promote and stabilize the formation of microtubules. The formation of …

Characterizing The Human Metapneumovirus Matrix Protein And Its Role In Infection, Chase J. Heim

Theses and Dissertations--Molecular and Cellular Biochemistry

Human metapneumovirus (HMPV) causes severe respiratory tract infections in all cohorts, but especially in vulnerable groups such as children, older adults, and the immunocompromised. The matrix (M) protein of HMPV, like the M proteins of other members of the Mononegavirales order, is involved in virus assembly and budding. However, other functions of the HMPV M protein have yet to be elucidated. To investigate these various functions of the M protein, we used a peptide-conjugated phosphorodiamidate morpholino oligomer (PPMO) antisense agent designed to block translation of the M gene to reduce M expression during infection. Treatment with varying concentrations of an …