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Application Of Near Infrared Reflectance Spectroscopy (Nirs) For Macronutrients Analysis In Alfalfa (Medicago Sativa L.), A. Morón, D. Cozzolino

IGC Proceedings (1977-2023)

Near infrared reflectance spectroscopy was used to assess the mineral composition of alfalfa (Medicago sativa L.) as a tool for nutritional diagnosis. One hundred and ninety four (n = 194) samples of alfalfa from different locations representing a wide range of soils were used. Samples were reflectance scanned in a NIRS 6500 (NIRSystems, USA) instrument. The coefficients of determination (R²) of the regression estimate of the concentration of nitrogen, calcium, phosphorus, potassium, magnesium and sulphur and the errors in cross validation (SECV) were 0.93 (SECV: 1.6), 0.95 (SECV: 1.3), 0.93 (SECV: 1.9), 0.88 (SECV: 2.8), 0.82 (SECV: …

Phosphorus, Sulphur And Micronutrients On Grassland Improvement With White Clover (Trifolium Repens L.) On Basaltic Soils In Uruguay, A. Morón, D. F. Risso

IGC Proceedings (1977-2023)

Grasslands improvement with legumes is a promising technology for increasing forage quality and productivity on basaltic soils, but requires the correction of nutrient deficiencies. Phosphorus is a key element, but usual techniques of soil analysis are not good predictors. An experimental work was conducted in the northern region of the country. Evaluating the response of an improvement with white clover to P. Citric Acid showed a good association between soil P status and legume behavior. In addition there was a positive trend in forage production, in relation to S application, even though this effect was only significant in legume yield …

Effect Of Liquid Cattle Manure Application On The Botanical Composition Forage Dry Yield And Soil Chemical Characteristics In Mixed Pastures, J. K. Lee, W. H. Kim, D. E. Shin, S. Seo, G. J. Choi, E. S. Chung, G. J. Park

IGC Proceedings (1977-2023)

Many farmers apply liquid cattle manure on grassland, often in excess of crop N requirement, resulting in groundwater contamination. Our experiment was carried out to determine the effect of liquid cattle manure application on the botanical composition, forage dry yield and soil chemical characteristics in mixed pastures at the forage experimental field, National Livestock Research Institute, RDA, Korea. Five treatments consisting of no fertilizer, chemical fertilizer (210kg N/ ha), liquid cattle manure (40, 60 and 80 ton N/ ha) were arranged in a randomized complete block design with three replicates. No fertilizer treatment consistently had lower botanical composition rate of …

Nutrient Dynamics And Inventory In Tropical Grassland Ecosystem In Southern India, K. Karunaichamy, Kailash Paliwal

IGC Proceedings (1977-2023)

The present study was to ascertain the distribution of calcium (Ca), magnesium (Mg) and sodium (Na) in the different plant components and in the soil top 30 cm at Kalikesam in Western Ghats region of southern India. Magnesium concentration increased in the order: dead shoots > belowground > litter > aboveground live. Sodium concentration in live shoots was maximum in February (0.51%) and minimum in August (0.05%). Annually 8.94 g/m² Ca, 2.41g/ m² Mg and 2.30g/ m² Na was taken up by plants. The distribution of the three nutrients in plant/soil system indicated that the major portion of the nutrients …

The Effect Of Fermented Saw-Dust Pig Manure On Forage Mineral Contents And Soil Fertility In Cheju Volcanic Ash Soil, M. C. Kim, T. G. Kim

IGC Proceedings (1977-2023)

Studies were carried out to investigate the change of soil characteristics and pasture minerals as affected by fermented saw-dust pig manure(FSM) applied to mixed pasture of a brown volcanic ash soil during the period from October 1996 to October 1998. Pasture mixed with orchardgrass (Dactylis glomerata L.) and white clover (Trifolium repens L.) was treated with N 200kg/ha + P 300kg/ha +K 200kg/ha(T1), N 100kg/ha + 10 ton/ha FSM(T2), FSM 10 ton/ha(T3), 20ton/ha FSM(T4), 40ton/ha FSM(T5) or 80ton/ha FSM(T6). K and P contents of mixed pasture in the 3rd year after sowing increased significantly with increasing levels of …

Nitrogen Budgets On A Pasture Under Combined Utilization Of Grazing And Cutting, Y. Kurokawa, S. Yamamoto, Y. Iijima, M. Kitagawa, F. Omata, S. Sakamoto, H. Tanaka, S. Suzuki, T. Shioya

IGC Proceedings (1977-2023)

Nitrogen budgets on a pasture being utilized for both grazing and cutting were investigated. A pasture containing tallfescue, orchard grass and white clover was divided into two areas: initially grazed (IA, 0.41ha) and grazed after cutting for hay (BA, 0.49ha). A herd of 4-5 cattle (heifers or steers of Japanese Black or Holstein) grazed at IA from 22 Apr. to 2 Jul. and grazed at both IA and BA from 3 Jul. to 21 Oct. 1999. The amount of N grazed from IA was 3 times higher than the sum of N of grazed herbage and harvested hay from BA. …

Effect Of Phosphatic Fertilizer Rates Level On Growth, Crude Protein Content And Nitrogen Fixation Ability Of Alfalfa At Sowing Year, Chang-Ho Kim, Hyo-Won Lee

IGC Proceedings (1977-2023)

The experiment was conducted to determine effects of phosphate fertilizers on growth characteristics, crude protein content and nitrogen fixation ability of alfalfa (Medicago sativa L) in the sowing year. Two rates of phosphate (200 and 400 kg/ha P) were applied to phosphorus -deficient native soil in Korea and samples were taken every 7 days from the 30th to the 93rd day. Number of branches, root length and dry matter weight at phosphate fertilizer level of 400kg in sowing year more increased than those at 200kg/ha. The number of nodules did not differ due to phosphate fertilizer rate. The crude …

Phosphorus Fertilizer Requirements For Temperate Dairy Pastures And Milk Production In South Eastern Australia, C. J. P. Gourley, I. Awty, M. O'Doherty, S. R. Aarons

IGC Proceedings (1977-2023)

Phosphorus (P) fertilizer is required to sustain productive pastures in Australia, yet optimum P fertility is poorly defined. A large farmlet study involving 10 herds was established in 1995 to determine the relationship between milk production and pasture response to four different P fertilizer rates, at three stocking rates. Pasture growth significantly increased with increasing P fertilizer rate. Cumulative milk production from pasture over 4 years, was significantly affected by P fertilizer, but responses diminished with increasing P rate. The results from this study will enable a better targeting of P fertility levels for profitable milk production from temperate dairy …

Responses To Nitrogen Fertilizer In Dairy Pastures With Differing Phosphorus Fertility In South Eastern Australia, C. J. P. Gourley, H. Frame, M. E. Hall

IGC Proceedings (1977-2023)

The application of nitrogen (N) fertilizer to temperate pastures is an increasingly popular management tool for boosting pasture production on dairy farms in Australia. However, limited information is available about N fertilizer responses that can be obtained from pastures with varying levels of phosphorus (P) fertility. A field experiment examining the initial and residual response of pasture growth to urea was carried out within a large dairy farmlet study, in October 1998. The four P fertility treatments (Olsen P values) ranged from 9 to 32 mg/kg. Dry matter yields increased with increasing N rates in all treatments, at both harvests. …

Comparison Of Nitrate Contents For Reed Canarygrass And Orchardgrass In Large Amounts Of Compound Fertilization, A. Fushimi, Y. Tamura, K. Matoba

IGC Proceedings (1977-2023)

Reed canarygrass (Phalaris arundinacea L.) could be an ideal candidate for manure application. We compared its growth, crude protein (CP) and nitrate N (NO³-N) content to that of orchardgrass (Dactylis glomerata L.) on different levels of nitrogen fertilization (3,10,15 and 20g N m^-2, applied from May 15 to September 15 after each monthly cutting. The dry weight (DW) of reed canarygrass increased significantly with increasing amounts of N nitrogen on July, August and September. However, orchardgrass only showed a significant increase in yield on September. The concentration of CP of reed canarygrass was significantly …

Modelling Nitrogen Uptake In Winter Oilseed Rape By Using Influx Kinetics Of Nitrate Transport Systems, S. Faure, P. Malagoli, P. Lainé, E. Le Deunff, F. Ledily, A. Ourry, J. Boucaud

IGC Proceedings (1977-2023)

A mechanistic model was proposed in order to predict nitrogen uptake by a culture of oilseed rape (Brassica napus L.), using independently measured characteristics of plants growing in hydroponic or under field conditions. Uptake kinetics of the different components (Constitutive and Inducible) of the Low and High Affinity Transport Systems of nitrate (CLATS, ILATS, CHATS and IHATS, respectively) were determined by ¹⁵NO₃^- labelling in controlled conditions. The use of kinetic equations of transport systems and the experimental field data from the INRA-Châlons rape databank allowed to model NO₃^- uptake during the plant growth cycle. …

Biological Interactions In Grassland Soils And Productivity, R. Cook, R. D. Bardgett, W. R. Eason, L. Skøt, K. J. Webb

IGC Proceedings (1977-2023)

This paper describes research on interactions between grassland plant species and soil microorganisms. Both parasitic and symbiotic microorganisms modify nutrient transfers between plants and soil. Experiments are described in which nematode infection of clover increased nitrogen transfer to companion ryegrass plants. Infection of clover enhanced activity of soil bacterial and fungal communities. Legume genotypes differing only in responses to symbionts (rhizobium and arbuscular mycorrhizal fungi) and pathogens are being developed for studies of gene expression during establishing and functional symbioses. Such plants can be used in experiments as defined perturbations that will provide information on the interactions and functions of …

Nitrogen Balances In High Rainfall, Temperate Dairy Pastures Of South Eastern Australia, Richard J. Eckard, D. F. Chapman, R. E. White, A. Smith, D. Chen, P. A. Durling

IGC Proceedings (1977-2023)

Nitrogen (N) fertilizer use on dairy pastures in south eastern Australia has increased exponentially over the past 15 years, causing increasing environmental concerns. Volatilisation, denitrification and leaching of N were measured for one year (1998-1999) in pastures receiving no N fertilizer (grass/clover), or 200 kg N/ha applied as urea (46%N) or ammonium nitrate (34.5%N). Nitrogen balances were calculated for each treatment.

Significantly more N was lost through volatilisation and denitrification when N was applied as urea compared to ammonium nitrate. Nitrate leaching losses were significantly greater with the application of N fertilizer, although the maximum loss was only 4.1 kg …

Design, Synthesis, And Preliminary Evaluation Of A Potential Synthetic Opioid Rescue Agent, Sidnee L. Hedrick, Dan Luo, Sophia Kaska, Kumar Kulldeep Niloy, Karen Jackson, Rupam Sarma, Jamie Horn, Caroline Baynard, Markos Leggas, Eduardo R. Butelman, Mary Jeanne Kreek, Thomas E. Prisinzano

Pharmaceutical Sciences Faculty Publications

BACKGROUND: One of the most prominent opioid analgesics in the United States is the high potency agonist fentanyl. It is used in the treatment of acute and chronic pain and as an anesthetic adjuvant. When used inappropriately, however, ingestion of just a few milligrams of fentanyl or other synthetic opioid can cause opioid-induced respiratory depression (OIRD), often leading to death. Currently, the treatment of choice for OIRD is the opioid receptor antagonist naloxone. Recent reports, however, suggest that higher doses or repeated dosing of naloxone (due to recurrence of respiratory depression) may be required to reverse fully fentanyl-induced respiratory depression, …

Smartphone Use And Psychological Well-Being Among College Students In China: A Qualitative Assessment, Cheng Dai, Zixue Tai, Shan Ni

Journalism and Media Faculty Publications

Background: Problematic smartphone use is widespread, and college-age youth faces an especially high risk of its associated consequences. While a promising body of research has emerged in recent years in this area, the domination of quantitative inquiries can be fruitfully and conceptually complemented by perspectives informed through qualitative research. Toward that end, this study aimed to interrogate the myriad behavioral, attitudinal, and psychological tendencies as a side effect of college students’ engagement with the smartphone in their everyday lived experience through in-depth interviews.

Methods: We recruited 70 participants from seven college campuses hailing from different geographic regions in China, and …

Improving The Methodology For Identifying Mild Cognitive Impairment In Intellectually High-Functioning Adults Using The Nih Toolbox Cognition Battery, Grant L. Iverson, Justin E. Karr

Psychology Faculty Publications

Objective: Low scores on neuropsychological tests are considered objective evidence of mild cognitive impairment. In clinical practice and research, it can be challenging to identify a cognitive deficit or mild cognitive impairment in high-functioning people because they are much less likely to obtain low test scores. This study was designed to improve the methodology for identifying mild cognitive impairment in adults who have above average or superior intellectual abilities.

Method: Participants completed the National Institutes of Health Toolbox for the Assessment of Neurological and Behavioral Function Cognition Battery (NIHTB-CB). The sample included 384 adults between the ages of 20 and …

Interventional Spine And Pain Procedure Credentialing: Guidelines From The American Society Of Pain & Neuroscience, Ramana K. Naidu, Rahul Chaturvedi, Alyson M. Engle, Pankaj Mehta, Brian Su, Krishnan Chakravarthy, Kasra Amirdelfan, Jeffrey Henn, Dawood Sayed, Jay S. Grider, Timothy Deer

Anesthesiology Faculty Publications

Background: The discipline of interventional pain management has changed significantly over the past decade with an expected greater evolution in the next decade. Not only have the number of procedures increased, some of the procedures that were created for spine surgeons are becoming more facile in the hands of the interventional pain physician. Such change has outpaced academic institutions, societies, and boards. When a pain physician is in the credentialing process for novel procedure privileges, it can leave the healthcare system in a challenging situation with little to base their decision upon.

Methods: This paper was developed by a consensus …

Real-World Evaluation Of Universal Germline Screening For Cancer Treatment-Relevant Pharmacogenes, Megan L. Hutchcraft, Nan Lin, Shulin Zhang, Catherine Sears, Kyle Zacholski, Elizabeth A. Belcher, Eric B. Durbin, John L. Villano, Michael J. Cavnar, Susanne M. Arnold, Frederick R. Ueland, Jill M. Kolesar

Pathology and Laboratory Medicine Faculty Publications

The purpose of this study was to determine the frequency of clinically actionable treatment-relevant germline pharmacogenomic variants in patients with cancer and assess the real-world clinical utility of universal screening using whole-exome sequencing in this population. Cancer patients underwent research-grade germline whole-exome sequencing as a component of sequencing for somatic variants. Analysis in a clinical bioinformatics pipeline identified clinically actionable pharmacogenomic variants. Clinical Pharmacogenetics Implementation Consortium guidelines defined clinical actionability. We assessed clinical utility by reviewing electronic health records to determine the frequency of patients receiving pharmacogenomically actionable anti-cancer agents and associated outcomes. This observational study evaluated 291 patients with …

Predicting The Effect Of Indirect Cell Kill In The Treatment Of Multiple Brain Metastases Via Single-Isocenter/Multitarget Volumetric Modulated Arc Therapy Stereotactic Radiosurgery, Allison N. Palmiero, Denise Fabian, Marcus E. Randall, William St. Clair, Damodar Pokhrel

Radiation Medicine Faculty Publications

PURPOSE: Due to spatial uncertainty, patient setup errors are of major concern for radiosurgery of multiple brain metastases (m-bm) when using single-isocenter/multitarget (SIMT) volumetric modulated arc therapy (VMAT) techniques. However, recent clinical outcome studies show high rates of tumor local control for SIMT-VMAT. In addition to direct cell kill (DCK), another possible explanation includes the effects of indirect cell kill (ICK) via devascularization for a single dose of 15 Gy or more and by inducing a radiation immune intratumor response. This study quantifies the role of indirect cell death in dosimetric errors as a function of spatial patient setup uncertainty …

The Regulation Landscape Of Mapk Signaling Cascade For Thwarting Bacillus Thuringiensis Infection In An Insect Host, Zhaojiang Guo, Shi Kang, Qingjun Wu, Shaoli Wang, Neil Crickmore, Xuguo Zhou, Alejandra Bravo, Mario Soberón, Youjun Zhang

Entomology Faculty Publications

Host-pathogen interactions are central components of ecological networks where the MAPK signaling pathways act as central hubs of these complex interactions. We have previously shown that an insect hormone modulated MAPK signaling cascade participates as a general switch to trans-regulate differential expression of diverse midgut genes in the diamondback moth, Plutella xylostella (L.) to cope with the insecticidal action of Cry1Ac toxin, produced by the entomopathogenic bacterium Bacillus thuringiensis (Bt). The relationship between topology and functions of this four-tiered phosphorylation signaling cascade, however, is an uncharted territory. Here, we carried out a genome-wide characterization of all the MAPK orthologs …

Hiv Infection And The Risk Of World Health Organization-Defined Sudden Cardiac Death, Matthew S. Freiberg, Meredith S. Duncan, Charles Alcorn, Chung-Chou H. Chang, Suman Kundu, Asri Mumpuni, Emily K. Smith, Sarah Loch, Annie Bedigian, Eric Vittinghoff, Kaku So-Armah, Priscilla Y. Hsue, Amy C. Justice, Zian H. Tseng

Biostatistics Faculty Publications

Background

People living with HIV have higher sudden cardiac death (SCD) rates compared with the general population. Whether HIV infection is an independent SCD risk factor is unclear.

Methods and Results

This study evaluated participants from the Veterans Aging Cohort Study, an observational, longitudinal cohort of veterans with and without HIV infection matched 1:2 on age, sex, race/ethnicity, and clinical site. Baseline for this study was a participant's first clinical visit on or after April 1, 2003. Participants were followed through December 31, 2014. Using Cox proportional hazards regression, we assessed whether HIV infection, CD4 cell counts, and/or HIV viral …

Heavy Metal And Contaminant Loading In The Oder River Floodplains And Consequences For Livestock Management, G. Schalitz, A. Behrendt

IGC Proceedings (1977-2023)

The predominately pristine lower Oder floodplains have experienced sedimentation and enrichment of heavy metals and contaminants since the start of industrialization in the region. With the creation of the German-Polish National Park, which included the flooded grassland area, intensive milk production has been prohibited. The objective of this research was to estimate the impact of the floodplain heavy metal and contaminant levels on the lower Oder region. Soil and plant content were analyzed for three transects according to accepted DIN methods. High content in soil did not consistently result in high plant contents; however, higher content levels were often observed …

Extended Daylength To Increase Fall/Winter Yields Of Warm-Season Perennial Grasses, P. Mislevy, T. R. Sinclair, J. D. Ray

IGC Proceedings (1977-2023)

Low forage production in fall/winter months is a severe limitation for dairy and beef cattle producers in the southeastern U.S. It was hypothesized that shrt daylengths during these months induce a physiological dormancy in grasses. Four grasses [Pensacola bahiagrass, Paspalum notatum Flugge; Tifton 85 and Florakirk bermudagrass, Cynodon dactylon (L.); Florona stargrass, C. nlemfuensis Vanderyst var. nlemfuensis] were subjected to extended daylengths during the winter/fall months in a field test. Pensacola bahiagrass and Tifton 85 bermudagrass showed especially dramatic increases in forage yield during the fall/winter season under the extended daylength. Genetic elimination of daylength sensitivity in these grasses …

Nitrogen Or Water Limitation To Grassland Growth During A Moderate Dry Season In The Humid Tropics, P. Cruz, M. Boval

IGC Proceedings (1977-2023)

The aim of this work was to compare the effects of nitrogen and water shortage on grassland production during a moderate dry season in a tropical humid environment. The results of two experiments carried out in Guadeloupe (FWI) have shown that during dry spells, the reduction in growth of natural grasslands based on Angleton grass (Dichantium aristatum) is more easily corrected by nitrogen fertiliser application than by irrigation. This shows that it is not the availability of water, but that of nitrogen, which is limiting the primary production of Angleton grass, stands. This result is less clear for …

Screening Brachiaria Introductions For Resistance To Spittlebugs (Homoptera: Cercopidae), José R. Valério, Cacilda Borges Do Valle, A. P. De Souza, M. C. M. Oliveira

IGC Proceedings (1977-2023)

Spittlebugs are the most evident and damaging pests of Signal grasses (Brachiaria) in tropical America. Damage caused by these insects can result in the complete loss of available forage, thereby reducing the carrying capacity of infested pastures. Host plant resistance is a low-cost method of controlling insects. High level of spittlebug resistance is found in the cultivar Marandu (B. brizantha), but it requires more fertile soils. Brachiaria germplasm provided by the International Center for Tropical Agriculture (CIAT) is available at National Beef Cattle Research Center of the Brazilian Agricultural Research Corporation (Embrapa Beef Cattle) and it …

Effects Of Remote Digital Monitoring On Oral Hygiene Of Orthodontic Patients: A Prospective Study, Linda Sangalli, Fabio Savoldi, Domenico Dalessandri, Stefano Bonetti, Min Gu, Alberto Signoroni, Corrado Paganelli

Oral Health Science Graduate Research

BACKGROUND: Remote digital monitoring during orthodontic treatment can help patients in improving their oral hygiene performance and reducing the number of appointments due to emergency reasons, especially in time of COVID-19 pandemic where non-urgent appointments might be discouraged.

METHODS: Thirty patients scheduled to start an orthodontic treatment were divided into two groups of fifteen. Compared to controls, study group patients were provided with scan box and cheek retractor (Dental Monitoring®) and were instructed to take monthly intra-oral scans. Plaque Index (PI), Gingival Index (GI), and White Spot Lesions (WSL) were recorded for both groups at baseline (t₀), every …

A Call To Action Toward Optimizing The Electrical Dose Received By Neural Targets In Spinal Cord Stimulation Therapy For Neuropathic Pain, Krishnan Chakravarthy, Rajiv Reddy, Adnan Al-Kaisy, Thomas Yearwood, Jay S. Grider

Anesthesiology Faculty Publications

Spinal cord stimulation has seen unprecedented growth in new technology in the 50 years since the first subdural implant. As we continue to grow our understanding of spinal cord stimulation and relevant mechanisms of action, novel questions arise as to electrical dosing optimization. Programming adjustment — dose titration — is often a process of trial and error that can be time-consuming and frustrating for both patient and clinician. In this report, we review the current preclinical and clinical knowledge base in order to provide insights that may be helpful in developing more rational approaches to spinal cord stimulation dosing. We …

Random Forest-Integrated Analysis In Ad And Late Brain Transcriptome-Wide Data To Identify Disease-Specific Gene Expression, Xinxing Wu, Chong Peng, Peter T. Nelson, Qiang Cheng

Sanders-Brown Center on Aging Faculty Publications

Alzheimer's disease (AD) is a complex neurodegenerative disorder that affects thinking, memory, and behavior. Limbic-predominant age-related TDP-43 encephalopathy (LATE) is a recently identified common neurodegenerative disease that mimics the clinical symptoms of AD. The development of drugs to prevent or treat these neurodegenerative diseases has been slow, partly because the genes associated with these diseases are incompletely understood. A notable hindrance from data analysis perspective is that, usually, the clinical samples for patients and controls are highly imbalanced, thus rendering it challenging to apply most existing machine learning algorithms to directly analyze such datasets. Meeting this data analysis challenge is …

Associations Of Circulating Dimethylarginines With The Metabolic Syndrome In The Framingham Offspring Study, Ibrahim Musa Yola, Carlee Moser, Meredith S. Duncan, Edzard Schwedhelm, Dorothee Atzler, Renke Maas, Juliane Hannemann, Rainer H. Böger, Ramachandran S. Vasan, Vanessa Xanthakis

Biostatistics Faculty Publications

BACKGROUND: Circulating levels of the endogenous inhibitor of nitric oxide synthase, asymmetric dimethylarginine (ADMA), are positively associated with the prevalence of metabolic syndrome (MetS) in cross-sectional investigations. It is unclear if circulating ADMA and other methylarginines are associated with incident MetS prospectively.

METHODS: We related circulating ADMA, symmetric dimethylarginine (SDMA), L-arginine (ARG) concentrations (measured with a validated tandem mass spectrometry assay) and the ARG/ADMA ratio to MetS and its components in 2914 (cross-sectional analysis, logistic regression; mean age 58 years, 55% women) and 1656 (prospective analysis, Cox regression; mean age 56 years, 59% women) individuals from the Framingham Offspring Study …

Selection Of High Yielding And Anthracnose Resistant Stylosanthes For Brazil, India And China, N. White, S. Chakraborty, Celso D. Fernandes, M. J. Charchar, N. P. Melkania, C. R. Ramesh, B. Gangaiah, Guodao Liu, D. F. Cameron, J. W. Miles

IGC Proceedings (1977-2023)

Resistance to anthracnose, dry matter yield (DMY) and seed yield (SY) was assessed for germplasm and breeding lines of Stylosanthes in Brazil, India and China. Overall, Stylosanthes guianensis produced higher DMY than S. scabra, S. capitata and S. macrocephala at most sites in Brazil. Data from China suggest that there are high yielding anthracnose resistant S. guianensis lines that can reduce the reliance on CIAT 184. S. seabrana might also prove successful. S. seabrana in India produced the highest DMY and SY and it can form nodules with native Bradyrhizobium strains. Regional differences in resistance within accessions stress the …