Dissecting The Role Of Actin-Myosin Motor And Calcium – Based Organelle Secretion In Motility Of Sarcocystis Neurona, Parul Suri

Theses and Dissertations--Veterinary Science

Sarcocystis neurona is a protozoan parasite that causes a rare neurological disorder in horses called Equine Protozoal Myeloencephalitis (EPM). Apicomplexa use actin-myosin based motor and organelle secretion to interact with the host cell and invade it. Despite the importance of motility and invasion-needed factors, the mechanisms by which S. neurona employs host cell association strategies to interact remains largely undefined. To address this knowledge gap, we hypothesize that just like other Apicomplexa, S. neurona utilizes actin polymerization for substrate-dependent gliding. Moreover, we also hypothesize that micronemes of S. neurona secrete proteins that are calcium-dependent. Based on previous studies in T. …

The Effects Of Cannabidiol On Senior Equine Health, Shelley Turner

Theses and Dissertations--Veterinary Science

Cannabis sativa L., has been revealed to produce hundreds of phytocannabinoids, of which cannabidiol (CBD) is one of the most desired. It has been revealed that CBD can potentially treat inflammation and act as an analgesic in humans without psychoactive effects of delta-9-tetrahydrocannabinol (THC). Recently, there has been interest in understanding the potential health benefits of CBD for horses. With an increasing senior horse population (< 15 years old), alternatives to the use of non-steroidal anti-inflammatory drugs (NSAIDs) such as phenylbutazone, firocoxib, and flunixin meglumine, are desired as these common anti-inflammatory treatments have negative side effects. Because senior horses may have chronic low-grade inflammation, termed inflamm-aging, they are a natural model to investigate the efficacy of CBD on inflammatory responses as well as other health parameters. Given this interest, and due to the lack of research conducted on CBD in the horse, the overall objective of this dissertation work was to conduct both basic and applied research investigating the effects of CBD on equine immune function and health. The first objective was to determine the in vitro effects of CBD as an anti-inflammatory. Peripheral blood mononuclear cells (PBMCs) from senior horses were cultured in vitro with increasing concentrations of pure CBD dissolved in dimethyl sulfoxide (DMSO). These cells were …

The Microbiome Of The Equine Roundworm, Parascaris Spp., Jennifer Lynn Cain

Theses and Dissertations--Veterinary Science

Parasitic nematodes, including the large roundworms colloquially known as ascarids, affect the health and well-being of livestock animals worldwide. The equine ascarid, Parascaris spp., was the first ascarid parasite to develop wide-spread anthelmintic drug resistance, with other species slowly following suit. There are no new classes of anthelmintics currently in development, and a solution to the ever-increasing prevalence of resistance is desperately needed. The microbiome has been shown to be an important factor in the fitness and health of many organisms and changes to microbiome composition have been associated with a plethora of diseases. The microbiome is also important to …

Evaluating Restoration Outcomes: Red Spruce Reforestation In The West Virginia Highlands, Benjamin Rhodes

Theses and Dissertations--Forestry and Natural Resources

Red spruce (Picea rubens) is the keystone species of the red spruce-northern hardwood forest, a unique high-elevation plant community that supports hundreds of animal species in the Central Appalachians. These forests were devastated by the Industrial Logging Era of the late 1800s and early 1900s, and they remain in a degraded and fragmented state. Current restoration efforts include red spruce plantings on old field sites and reclaimed coal mines. This project seeks to aid those efforts by evaluating restoration outcomes for vegetation and soils along a ten-year chronosequence. Specifically, the study aims to determine whether restoration site soils …

Eight-Year Regeneration Response To Gap-Based Irregular Shelterwood Practices In Oak Dominated Stands Near Berea, Kentucky, Brooke Elizabeth Gauthier

Theses and Dissertations--Forestry and Natural Resources

This research examined regeneration dynamics following gap-based irregular shelterwood practices in intermediate productivity oak (Quercus)-dominated stands located in Berea College Forest, Berea, KY, USA. Established in 2012, the study includes 12 experiment units comprising a circular harvest gap (30 m radius) and a matrix zone delineated around the circumference of the gap, which extended 30 m from the gap edge into the adjacent forest. A midstory removal treatment was performed within six of the experiment units’ matrices, while six matrices were left untreated (control). Belt transects extending 60 m from gap center to the end of the matrix zone were …

Spatial Ecology Of Rocky Mountain Elk (Cervus Canadensis Nelsoni) Cows In Southeastern Kentucky, Nathan Dennis Hooven

Theses and Dissertations--Forestry and Natural Resources

The elk (Cervus canadensis) was extirpated from its range in eastern North America by the end of the 1800s, prompting several U.S. states and Canadian provinces to begin translocation programs with the goal of reestablishing elk populations. While eastern elk managers have relied on information from western herds to guide population and habitat management, there is a need for region-specific research on the spatial ecology and habitat associations of translocated elk given the stark differences in landscape, climate, predator communities, and harvest regimes across the continent. While the Kentucky elk reintroduction is one of the best documented programs …

Bat Activity On West Virginia Mined Lands Restored Via The Forestry Reclamation Approach, Briana C. Snyder

Theses and Dissertations--Forestry and Natural Resources

The Forestry Reclamation Approach (FRA) is a practical guide to reforesting surface mined lands. Bats, as a unique group of species with declining populations, could benefit from this reforestation. In order to determine if the FRA is providing suitable bat foraging habitat, I surveyed bat activity at created depressional wetlands on 1-year old and 8-year old FRA restored lands (FRA1; FRA8), as well as at naturally formed wetlands in regenerating forest on traditionally reclaimed mined land (~40 years old; REGEN) and wetlands in mature forest not previously mined (MAT). I passively recorded echolocation calls for 12 nights across sixteen sites …

The Response Of Beef Cattle To Disturbances From Unmanned Aerial Vehicles (Uavs), Gabriel Abdulai

Theses and Dissertations--Biosystems and Agricultural Engineering

Unmanned aerial vehicles (UAVs) are increasingly becoming common in animal agriculture. However, research regarding the impact of UAV disturbance on animal wellbeing is lacking or limited. The goal of this study was to investigate the effect of UAV flights on beef cattle by measuring cattle heart and movement rate when introduced to single or multiple UAV flights. A total of 16 -18 crossbred beef heifers were introduced to different flights patterns at between 5 and 9 m above ground level (AGL) at approximately 1 to 2 m/s horizontal velocity for 4 weeks with flights repeated 3 days per week. Results …

Lignin Valorization Via Reductive Depolymerization Using Promoted Nickel Catalysts And Sub- And Supercritical Methanol, Julia Parker

Theses and Dissertations--Biosystems and Agricultural Engineering

While lignin has been regarded as the most promising renewable feedstock for the sustainable manufacture of aromatic compounds, lignin valorization is necessary to improve the economic viability of biorefineries. Reductive catalytic fractionation (RCF), which combines delignification and lignin depolymerization into a single stage while maintaining the structure and integrity of the cellulose component, has evolved as an effective method for processing biomass. The ability of Cu and Fe to promote the performance of a 20% Ni/alumina catalyst when converting native lignin to alkylphenols by RCF in sub- and supercritical methanol was tested. The effectiveness of lignin extraction as measured by …

Implications To Tap And Stream Water Chemistry Due To Variations In Sampling Locations And Watershed Land Use, Dayana Rodriguez

Theses and Dissertations--Biosystems and Agricultural Engineering

The Kentucky River Basin, which is in the Eastern Kentucky Coal Field region, is a rural region with sparse population distribution and a history of water quality impairments. The long history of mining activity in the Commonwealth has not only left a mark on the area’s economy, but it has also shaped the environment. Changes in land use from forested areas to surface mining have been shown to significantly change water chemistry and hydrology. To date, there is limited information regarding the potential exposure and introduction of heavy metals and sulfate entering rural water treatment plants, resulting in a notable …

Sediment Nitrogen Dynamics In Backwater Wetland Confluences Of A Regulated River, Gina Degraves

Theses and Dissertations--Biosystems and Agricultural Engineering

As harmful algal blooms in regulated river systems have increased in the past decade, the importance of understanding sediment nutrients has also increased. Research linking nutrient processes and fine sediment dynamics to harmful algal blooms in confluence wetlands along regulated rivers has recently become apparent. However, the relationship between sediment nutrient dynamics in confluence wetlands has been understudied. Utilization of sediment fingerprinting, high-frequency water quality monitoring, and tracer unmixing mass-balance modeling, was able to suggest sediment N mineralization in Appalachia confluence riparian wetland was not a dominate source of nitrate downstream. Further measures of supplementary tracers and additional sediment sources …

Classifying And Mapping Aquatic Vegetation In Heterogeneous Stream Ecosystems Using Visible And Multispectral Uav Imagery, Rozalia Agioutanti

Theses and Dissertations--Biosystems and Agricultural Engineering

The need for assessment and management of aquatic vegetation in stream ecosystems is recognized given the importance in impacting water quality, hydrodynamics, and aquatic biota. However, existing approaches to monitor are laborious and its currently not feasible to track spatial and temporal differences at broad scales. The objective of this study was therefore to map and classify aquatic vegetation of a shallow stream with heterogenous mixtures of emergent and submerged aquatic vegetation. Data was collected in the Camden Creek watershed within the Inner Bluegrass Region of central Kentucky. The use of unmanned aerial vehicles (UAVs) was employed and both visible …

Exploration Of Lignin-Based Superabsorbent Polymers (Hydrogels) For Soil Water Management And As A Carrier For Delivering Rhizobium Spp., Toby Adjuik

Theses and Dissertations--Biosystems and Agricultural Engineering

Superabsorbent polymers (hydrogels) as soil amendments may improve soil hydraulic properties and act as carrier materials beneficial to soil microorganisms. Researchers have mostly explored synthetic hydrogels which may not be environmentally sustainable. This dissertation focused on the development and application of lignin-based hydrogels as sustainable soil amendments. This dissertation also explores the development of pedotransfer transfer functions (PTFs) for predicting saturated hydraulic conductivity using statistical and machine learning methods with a publicly available large data set. A lignin-based hydrogel was synthesized, and its impact on soil water retention was determined in silt loam and loamy fine sand soils. Hydrogel treatment …

Assessing Machine Learning Utility In Predicting Hydrologic And Nitrate Dynamics In Karst Agroecosystems, Timothy Mcgill

Theses and Dissertations--Biosystems and Agricultural Engineering

Seasonal hypoxia in the Gulf of Mexico and harmful algal blooms experienced in many inland freshwater bodies is partially driven due to excessive nitrogen loading seen from agricultural watersheds. Within the Mississippi/Atchafalaya River Basin, many areas are underlain with karst features, and efforts to reduce nitrogen contributions from these areas have had varying success, due to lacking a complete understanding of nutrient dynamics in karst agricultural systems. To improve the understanding of nitrogen cycling in these systems, 35 months of high resolution in situ water quality and atmospheric data were collected and fed into a two-hidden layer extreme learning machine …

Effects Of Physiological Stage And Diet On Drosophila Suzukii Olfaction, Aerianna Shindindowi Littler

Theses and Dissertations--Entomology

Massive economic damage by spotted-wing drosophila (SWD), Drosophila suzukii, results from their unique egg laying behavior. Gravid SWD flies employ a complex suite of behaviors to find fruit with the optimum firmness and chemistry. We tested for SWD attraction to various fruits and found overripe raspberries to be most attractive to gravid flies which was unexpected since they oviposit in ripe fruit. We next tested the same choice assay in a T-maze and found that the results were comparable. We also found discrete odor profiles among the ripening stages. When testing for sugars and CO2 concentrations from field-collections, overripe …

Net Effects Of Wild And Managed Birds In Agroecosystems, Karina Garcia

Theses and Dissertations--Entomology

Agricultural intensification and expansion have reduced biodiversity in agroecosystems, jeopardizing the ecosystem services that nature provides to humans in these landscapes including wildlife-mediated pest control. Among these purveyors of ecosystem services are birds, who can provide services to agroecosystems by consuming arthropod pests. Yet some bird species also act as pests by consuming crops. Herein, I use molecular diet analysis through high-throughput sequencing on DNA extracted from bird fecal samples to examine birds’ diet and classify bird species as consumers of major strawberry pests (Lygus spp.). Additionally, I use targeted PCR approaches to determine which bird species consumed strawberries. …

Midgut Metamorphosis In The Yellow Fever Mosquito, Aedes Aegypti, Najla Mohammed Albishi

Theses and Dissertations--Entomology

Remodeling of larval tissues including the midgut is essential for the completion of metamorphosis in the yellow fever mosquito, Aedes aegypti (Diptera: Culicidae). The midgut structure and function have been extensively studied in adult Ae. aegypti, because adult females need to blood feed to reproduce, and the alimentary canal is the first barrier that encounters pathogens ingested through the blood meal. The midgut of Ae. aegypti undergoes remodeling during the larval-pupal metamorphosis. The degeneration of larval cells and the proliferation and differentiation of stem cells to form pupal-adult midgut are the major events that take place during metamorphosis. …

Rna Interference For Emerald Ash Borer Suppression: Ecotoxicological Assessment And Delivery Methods, Flavia Pampolini

Theses and Dissertations--Entomology

The emerald ash borer (EAB), Agrilus planipennis (Coleoptera: Buprestidae), is a highly invasive phloem-feeding insect native to Asia. It has killed millions of ash trees (Fraxinus spp.) since its accidental introduction into North America, causing profound economic and ecological impacts. RNA interference (RNAi) or double-stranded RNA (dsRNA)-mediated gene silencing is an emerging biotechnology that is developing as an innovative tool for pest management. Exogenous dsRNA triggers the RNAi pathway, silences genes, and disrupts protein function, causing insect mortality. RNAi has proven effective in reducing target gene expression and causing mortality in EAB; however, two aspects stand as barriers …

Gene Silencing Provides Insights Into Bark Beetle Biology And Creates Potential For Broad Scale Forest Protection, Beth R. Kyre

Theses and Dissertations--Entomology

Dendroctonus bark beetles are among the most economically and ecologically significant forest pests in North America and play a critical role in the overall health of conifer forest ecosystems. Dendroctonus bark beetles influence ecosystem benefits and biodiversity and drive forest succession, and adversely affect timber production, forest management, and recreation. As temperatures surge and climatic fluctuations become more extreme, catastrophic bark beetle outbreaks are increasing in frequency, escalating pressures on highly vulnerable conifer forests already compromised by heat and drought. Eruptive outbreaks of Dendroctonus beetles are largely unhindered by traditional silvicultural management; these practices further disrupt forest ecosystem services, including …

A Functional Genomics Approach To Overwintering Mechanisms In Insects, Emily Allison Wheeler Nadeau

Theses and Dissertations--Entomology

Insects living in temperate and polar environments have developed numerous adaptations to increase survival at low temperatures. A majority of insects are freeze-intolerant and die from internal ice formation, but some are freeze-tolerant and can survive ice formation in extracellular spaces. Both categories of insects prepare for winter with a combination of seasonal and rapid acclimation responses, which differ both in time course and in underlying mechanisms. In this dissertation, I examine adaptations for winter survival in several insect species with a specific focus on molecular mechanisms. To better understand the underpinning mechanisms of these adaptations, I leverage functional genomics …

Attentional Bias, Inhibitory Control, And Food Reinforcement As Mechanisms Promoting Eating Behavior After Exercise, Kylie F. L. Mcfee

Theses and Dissertations--Nutrition and Food Systems

Background: Excess weight and obesity are serious health conditions characterized by modifiable and nonmodifiable risk factors. Exercise is a common method for achieving weight loss; however, results are not always achieved due to post-exercise compensatory behaviors. Increased energy intake (EI) is thought to be the main compensatory behavior hindering weight loss among overweight and obese adults. It is important to determine and understand the mechanisms behind energy compensation following exercise, as this will allow for the discovery of future interventions that may provide individuals with ways to improve exercise as a weight loss treatment.

Objective: To determine if a single …

The Influence Of Sociopolitical Factors On Adolescents’ And Youths’ Development, Banafsheh Aghayeeabianeh

Theses and Dissertations--Family Sciences

Youths and adolescents are one of the major perpetrators of antisocial and deviant behaviors, which have deleterious consequences for both the perpetrators and society. Although there is extensive literature on youth and adolescent antisocial behavior, some correlates of youth antisociality are not known yet. As such, the present study is devoted to understanding the micro- and macro-level predictors of youth and adolescents’ antisociality in three contexts. Three empirical studies applying bioecological systems theory and analyzing data from the International Dating Violence database using Mixed Effects Models were conducted to investigate the ecology of the development of antisocial behaviors among youth. …

Personality And Coping In Response To Traumatic Experiences In Emerging Adult Women, Julia J. Beier

Theses and Dissertations--Family Sciences

Intimate dating violence is a common occurrence, especially among women (CDC, 2019). Because this type of trauma is so prevalent, it is important to explore how experiences of it impact women and how they cope with its effects. This study explored how individual differences impact the ways in which young women cope with trauma, as well as whether the type of trauma moderate the link between individual differences and coping strategies. Participants were 304 college-age women from a large university in the southeastern United States. Trauma was select items from the Trauma History Questionnaire, while coping was measured using the …

Pilot Study: The Role Of Online Grocery Shopping In Meal Planning & Prepartion: Utilizing Text Message Based Behavioral Nudges, Brittany Nikole Cox

Theses and Dissertations--Nutrition and Food Systems

Purpose: To examine if an online grocery shopping intervention improves meal planning and preparation and subsequent dietary intake. The primary hypothesis was that online shopping can improve meal planning and meal preparation habits while also improving dietary intake.

Method: Participants were randomized to either online only (O), online plus nutritional nudges (O+I), or standard brick and mortar (BM). Those in the O+I arm received behavioral nudges three (3) times per week and were part of a Facebook group assisting them with meal planning, nutrition education, and reminders to shop online. The O arm only received reminders to shop online, and …

Associations Of Sexual Satisfaction, Relationship Quality, And Cognitive Functioning Within Intimate Relationships In Older Adulthood, Allison G. Smith

Theses and Dissertations--Family Sciences

The roles of sexual satisfaction, relationship quality, and cognitive functioning within older adults (N = 538) intimate relationships are assessed at two timepoints over an 11-year timeframe. Longitudinal data from 63 older adults are assessed through a path modeling approach to examine how sexual satisfaction and relationship quality change throughout older adulthood. Cross-sectional data for 538 older adults are utilized to examine the association between cognitive health and sexual satisfaction/relationship quality. Qualitative data from 125 older adults are utilized to understand how older adults describe their intimate relationships. Results from path models demonstrate that one’s degree of sexual satisfaction …

Trauma-Sensitive Leadership In Multidisciplinary Settings, Jennifer Griswold Withrow

DSW Capstone Projects

As the social work profession continues to expand into more diversified areas of practice, such as politics, environmental justice, governmental work, and international crisis work, it is critical to ensure that social workers have the tools necessary to be successful leaders in these extended areas. Utilizing Trauma-Sensitive Leadership is an important concept to incorporate into multidisciplinary teams to ensure employees and communities feel supported and understood. By implementing these concepts which are ingrained into the social work profession into expanded areas of practice, social workers can feel empowered to lead multidisciplinary teams and provide support and understanding to the staff …

Patient Transitions Between Acute And Post-Acute Care Organizations: Can Nursing Communication Prevent Patient Readmission?, Penny Gilbert

DNP Projects

Preventing Patient Readmission

Background and Review of Literature: Communication is the foundation of patient safety. As patients move from the acute to post-acute care setting, risk for insufficient communication rises. Research demonstrates a vast array of communication hand-off tools currently exist for and between different care arenas. No one tool has been standardized for patients transitioning from acute to post-acute care settings. The Institute of Medicine (IOM) and The Joint Commission (TJC) have published multiple documents discussing communication plagues within health care resulting in readmission.

Purpose: The purpose of this project is multifaceted: 1) identifying current nursing communication practices between …

Improving The Healthcare Transition For Adolescents With Asthma: Implementing A Transition Readiness Assessment, Andrea Pauley

DNP Projects

BACKGROUND: Asthma is the most common pediatric chronic disease in the United States and adolescence represents the highest prevalence among all age groups. Transition planning is recommended for all adolescents, especially those with special health care needs (SHCN). Only 17% of adolescents with SHCN receive adequate transition planning.

PURPOSE: The purpose of this study was to implement the Transition Readiness Assessment Questionnaire (TRAQ) for adolescents age 12 years and older with asthma to identify the current level of transition readiness and identify factors associated with readiness.

METHODS: This study was an exploratory, cross-sectional design to evaluate and describe participants’ level …

An Ecological Study Of Glyphosate Use And Non-Hodgkin’S Lymphoma, Dexter Corlett, Steven R. Browning

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Glyphosate is currently the most widely used herbicide in the world. Initially thought to be non-carcinogenic in humans, in 2015 glyphosate was classified as a “probable carcinogen” by the International Agency for Research on Cancer due to several small epidemiological studies indicating a link between the pesticide and hematologic cancers, especially non-Hodgkin’s lymphoma (NHL). The current work is an ecological study using counties in Kentucky, Arkansas, and Iowa to compare glyphosate usage to NHL incidence using a multivariate Poisson regression. We found no significant correlation between glyphosate use and NHL incidence, though caution should be taken to draw significance from …

Utilizing A Video-Based Intervention To Improve Medicine Adherence And Retention Of Hiv Care In A Hiv Clinic., Robert Currie

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Memphis, Tennessee has higher rates of black men who have sex with men living with human immunodeficiency (BMSMLWH) compared to state and national levels. ART has been shown to be effective in keeping people living with HIV virally suppressed and unable to transmit HIV through sex. However, BMSMLWH are disproportionately affected by HIV than other races in Memphis, Tennesee. The proposed program relies on evidence-based data that demonstrates the effectiveness of video-based intervention in improving medication adherence and HIV retention of care. Taking Care of Me is a video-based intervention that promotes HIV treatment initiation, ART adherence, and retention in …