Digital Commons Network^™

Abnormal Invasive Hemodynamics In Heart Transplant Recipients, Navin Rajagopalan, Donna R. Dennis, Julia Akhtarekhavari, Kenneth Campbell

Physiology Faculty Publications

BACKGROUND Few studies have quantified invasive hemodynamic parameters in post heart transplant recipients. AIM To report the incidence of abnormal hemodynamics in heart transplant recipients at 1-year and 3-year post-transplant and determine if there was any correlation with recipient and donor characteristics. METHODS Data from 279 consecutive heart transplant recipients from 2007 through 2020 were analyzed. Clinical variables regarding both recipients and donors as well as hemodynamic variables obtained via right heart catheterization during 1-year and 3-year annual testing were recorded. Simple and multiple linear regression tests were used to determine how recipient and donor variables influenced hemodynamic parameters at …

Iodinated Disinfection Byproduct Formation From Iohexol In Sunlit And Chlorinated Urban Wastewaters, Reagan Patton Witt, Marcelo I. Guzman

Chemistry Faculty Publications

No abstract provided.

Person-Centered Trauma-Informed Care (Pctic): A Training Needs Assessment For Home And Community-Based Services In West Virginia, Ranjita Misra, A. Brianna Sheppard, Natalie Wilson, Megan C. Govindan, Matthew Myers, Amanda Acord-Vira, Steven Wheeler, Heather Henderson, Christena Ross, Laura Cooper, Rachel A. Goff

Journal of Appalachian Health

Introduction: Person-centered, trauma-informed care (PCTIC) is a combined, holistic service model promoting the well-being and empowerment of trauma survivors.

Purpose: This study examined the PCTIC knowledge, skills, and abilities needed by West Virginia (WV) Medicaid Home and Community-Based Services (HCBS) direct service professionals (DSPs) to serve clients that may have experienced trauma.

Methods: Semi-structured interviews were conducted with diverse stakeholders (n = 32) from 19 organizations that provide HCBS services in all 55 counties. Interviews focused on identifying current training, needs and knowledge gaps, and priority areas for additional PCTIC training. ATLAS.ti software was used to manage and code. Validity …

Magnolia Tapichalacaensis (Magnoliaceae), A New Species From The Andean Foothills Of Zamora Chinchipe, Ecuador, Álvaro J. Pérez, Edison Rea, Jennifer Martínez, Fanny Hidalgo, Franco Mendoza, Ramiro Mendoza, Nelson Jaramillo Díaz, Fredy Archila, Kevin S. Burgess, Rosana Zenil-Ferguson, J. Antonio Vázquez-García

Biology Faculty Publications

The number of new species of Ecuadorian Magnolia Linnaeus (1753: 535) continues to increase as exploration of the Andes progresses. Currently, 24 species of Magnolia are recorded for Ecuador (Vázquez-García et al. 2012, 2013, 2015a, 2015b, 2016, 2017, 2018, Arroyo et al. 2013a, 2013b, 2019, Pérez et al. 2016, 2023), which belong to M. sect. Splendentes Dandy in Vázquez (1994: 4) and Talauma Baillon (1866: 3) (Wang et al. 2020), the former consisting of six species, including the new species described here, and the latter 19 species. There are 17 endemic species in Ecuador, three of which also occur in …

Navigating Hepatitis C Care, Cheyenne R. Wagi, Renee Mcdowell, Anyssa Wright, Kathleen L. Egan, Christina S. Meade, April M. Young, Madison N. Enderle, Angela T. Estadt, Kathryn E. Lancaster

Epidemiology and Environmental Health Faculty Publications

Background Hepatitis C virus (HCV) and injection drug use among young women are dramatically rising in the rural United States. From 2004 to 2017, heroin use among non-pregnant women increased 22.4% biennially, mirroring increases in HCV cases, especially among younger populations. Young women who inject drugs (YWID, ages 18–35) face elevated HCV risk due to biological, behavioral, and socio-cultural factors. Barriers to HCV testing and treatment services further delay diagnoses, fuel transmission, and limit access to harm reduction services. This study applies the Theoretical Domains Framework (TDF) to identify factors influencing HCV testing and treatment among YWID in rural Appalachia …

The First Complete Assembly For A Lungless Urodelan With A “Miniaturized” Genome, The Northern Dusky Salamander (Plethodontidae: Desmognathus Fuscus), Edward A. Myers, Alexander A. Stewart, Aidan E. O’Brien, Jeramiah J. Smith, R. Alexander Pyron

Biology Faculty Publications

Salamanders have some of the largest genomes among animals, driven primarily by expansion of repeat elements and slow rates of DNA loss. However, species in the lungless genus Desmognathus (Plethodontidae) have some of the smallest genomes at ∼13 to 15 GB, though previous sequencing attempts with short-read assemblies were still fragmentary. Here, we assemble an annotated draft genome of Desmognathus fuscus using PacBio HiFi reads and transcriptomic data from 2 specimens from the same population. The combined dataset resulted in a 16.1 GB assembly in 19,632 contigs and an L/N50 of 2,459/1.74 MB, with the longest contig at 27.9 MB. …

Multidimensional Biocircuitry Of Exercise Adaptation, Jeremy S. Mcadam, Zachary A. Graham, S. Craig Tuggle, Kaleen M. Lavin, Marcas M. Bamman, Jeremy S. Mcadam, Zachary A. Graham, Brandon Peoples, Regina S. Seay, Kaleen M. Lavin, Amber B. Gargus, Sufen Yang, Samia M. O’Bryan, Devin J. Drummer, Christian J. Kelley, Margaret B. Bell, Inmaculada Aban, Gary R. Cutter, Marcas M. Bamman, Michael P. Craig, Jin Zhang, Akshay Hira, Timothy J. Broderick, Madhavi P. Kadakia, Zachary A. Graham, Kalyani Peri, Margaret B. Bell, Inmaculada Aban

Physiology Faculty Publications

In a randomized, dose-response trial, we used molecular and phenomic profiling to compare responses with traditional moder ate-intensity endurance and resistance training (TRAD) versus high-intensity tactical training (HITT) that encompassed explosive whole-body interval training and high-intensity resistance training. Ninety-four participants (18–27 yr) completed 12 wk of TRAD or HITT followed by 4 wk of detraining. Although similar performance and body composition improvements were observed in response to HITT and TRAD, some dose-dependent differences were observed for: 1) ex vivo muscle tissue changes in myofiber size, capillarization, satellite cell frequency, and mitochondrial function and 2) differential gene expression (DGE) of muscle …

Satellite Cells Choreograph An Immune Cell-Fibrogenic Cell Circuit During Mechanical Loading In Geriatric Skeletal Muscle, Nicholas T. Thomas, Camille R. Brightwell, Allison M. Owen, Alexander R. Keeble, Sabin Khadgi, Yuan Wen, Christopher S. Fry, Kevin A. Murach

Physiology Faculty Publications

Muscle stem cells, or satellite cells (SCs), decline in number throughout the lifespan and may become senescent in very old age. Whether and how remaining SCs contribute to muscle adaptation in the oldest-old is unclear. Using acute mechanical overload in geriatric SC replete and depleted mice (28-month-old) combined with single-cell RNA-sequencing, we show: (i) subsets of geriatric SCs display signs of senescence as well as normal fate progression during overload, (ii) SCs express markers that may contribute to the regulation of innervation, (iii) the presence of SCs during overload enhances global intercellular communication and increases mRNA levels of the cell …

Noncontact Diffuse Optical Imaging Of Blood Flow And Oxygenation Distributions In Reconstructive Rat Skin Flaps, Samaneh Rabienia Haratbar, Fatemeh Hamedi, Mehrana Mohtasebi, Faraneh Fathi, Faezeh Akbari, Dara Singh, L. I.U. Xuhui, Li Chen, Lesley Wong, Lei Chen, Guoqiang Yu

Surgery Faculty Publications

Perioperative identification of ischemic-hypoxic tissue is essential for reducing the risk of mastectomy skin flap necrosis (MSFN), a major complication following mastectomy. Implant-based breast reconstruction is jeopardized in the presence of MSFN. However, existing technologies are limited by depth insensitivity, dye-related risks, the need for contact sensors, and an inability to simultaneously assess both blood flow and oxygenation. A multi-wavelength speckle contrast diffuse correlation tomography (MW-scDCT) system was developed for noncontact, dye-free, and depth-sensitive imaging of tissue hemodynamics in a rat model incorporating four distinct flap scenarios: sham (SH), implant (IM), half necrosis (HN), and full necrosis (FN), representing varying …

Identification Of Metabolic Pathways And Hub Genes Associated With Ultrasound Subcutaneous Fat And Muscle Depth Of The Longissimus Muscle In Cull Beef Cows Using Gene Co-Expression Analysis, Harshraj Shinde, Kyle R. Mcleod, Jeffrey W. Lehmkuhler

Animal and Food Sciences Faculty Publications

Beef production is an important component of the world’s food supply, with production being near 59 million tons in 2023 (USDA, 2023). Enhancing our understanding of the factors influencing metabolism will lead to improvements in production efficiency. Using RNA-seq and WGCNA of longissimus muscle samples, gene expression and metabolic pathway analyses were performed to examine relationships with ultrasound and body mass variables. In this study, body weight (BW), ultrasound back fat (BF), ultrasound muscle depth (MD), and body condition score (BCS) were traits recorded for 18 cull beef cows. As expected, all production-related traits monitored (WT, BF, MD, and BCS) …

Danicamtiv Reduces Myosin’S Working Stroke But Activates The Thin Filament By Accelerating Actomyosin Attachment, Brent Scott, Lina Greenberg, Caterina Squarci, Kenneth S. Campbell, Michael J. Greenberg

Physiology Faculty Publications

Heart failure is a leading cause of death worldwide, and even with current treatments, the 5-y transplant-free survival rate is only ~50 to 70%. As such, there is a need to develop new treatments for patients that improve survival and quality of life. Recently, there have been efforts to develop small molecules for heart failure that directly target components of the sarcomere, including cardiac myosin. Danicamtiv is one of these molecules; however, its direct effects on myosin’s single molecule mechanics and kinetics are not well understood. Using optical trapping techniques, stopped flow transient kinetics, and in vitro reconstitution assays, we …

Dynamic Remodeling Of Centrioles And The Microtubule Cytoskeleton In The Lifecycle Of Chytrid Fungi, Alexandra F Long, Krishnakumar Vasudevan, Andrew Swafford, Claire M Venard, Jason E Stajich, Lillian K Fritz-Laylin, Jessica L Feldman, Tim Stearns

Biology Faculty Publications

Cell movement and division are complex behaviors driven by a dynamic internal cytoskeleton. The molecular components and principles of cytoskeletal assembly are well studied, but less is known about cytoskeletal remodeling events, including how centrioles transition from ciliary base to centrosome. Here we address this using the chytrid Rhizoclosmatium globosum, a zoosporic fungus which has centrioles and cilia, lost in most fungal lineages. Chytrids undergo reorganization of their microtubule cytoskeleton as they grow from zoospore to multinucleated coenocyte. We use evolutionary comparison, RNA sequencing, and expansion microscopy to understand this reorganization and further develop this organism as a model for …

Fournier Gangrene Is Associated With Increased Length Of Stay And Higher Healthcare Costs Compared To Non-Perineal Necrotizing Soft Tissue Infections, Hayato Mitaka, Kristen Mcquerry, Kelsey Karnik, Alexandre R. Marra, Toshio Naito, Patrick Ten Eyck, Paul G. Auwaerter, Yuji Yamada, Takaaki Kobayashi

Biostatistics Faculty Publications

Background: Fournier gangrene (FG) is a necrotizing soft tissue infection (NSTI) of the perineum. Recent retrospective studies from quaternary centers suggest improved outcomes and a potentially less aggressive clinical course for FG than non-perineal NSTIs. However, comprehensive nationwide data remain limited. Methods: This retrospective cohort study analyzed the National Inpatient Sample (2016-2020) to compare outcomes between FG and non-perineal NSTIs. Adult patients undergoing surgical debridement with a diagnosis of FG or NSTI were identified using ICD-10 codes. Outcomes included in-hospital mortality, length of stay (LOS), hospital costs, and home discharge rates. Multivariable regression analyses adjusted for patient demographics, comorbidities, and …

“Literally Made Me Cry”: The Impact Of Course Material Costs On University Of Kentucky Students, Stephen Krueger

Library Reports and White Papers

The Student Course Materials Cost Survey was administered September 16 – October 6, 2024. It was open to current students at the University of Kentucky (UK). The goals were to help UK Libraries better understand how the cost of course materials affects students at UK and to provide a snapshot of student spending across the university. The questions were based on similar research done at other institutions in Virginia (Virginia Course Materials Survey, 2021) and Florida (2022 Student Textbook and Instructional Materials Survey, 2022). Other work based on those surveys includes the 2023 Illinois Course Materials Survey (CARLI, 2023) and …

Evaluation Of Real-World Evidence To Assess Health Outcomes Related To Deprescribing Medications In Older Adults, Kaleen N. Hayes, Joshua David Niznik, Danijela Gnjidic, Frank Moriarty, Nha Tran, Antoinette B. Coe, Andrew R. Zullo, Sirui Zhang, Matthew Alcusky, Dimitri Bennett, Sirpa Hartikainen, Marie Laure Laroche, Xiaojuan Li, Joshua K. Lin, Jennifer L. Lund, Maurizio Sessa, Shahar Shmuel, Caroline Sirois, Denis Talbot, Miia Tiihonen, Xuerong Wen, Mouna J. Sawan, Daniela C. Moga

Epidemiology and Environmental Health Faculty Publications

Observational studies using real-world data (RWD) can address gaps in knowledge on deprescribing medications but are subject to methodological issues. Limited data exist on the methods employed to use RWD to measure the effects of deprescribing. To describe methodological approaches used in observational studies of deprescribing medications in older adults, we conducted a systematic review in Medline for observational studies published in English (January 1, 2000, to September 14, 2023) that examined the health effects of medication deprescribing in older adults. We described study characteristics and methods, focusing on the operationalization of deprescribing as an exposure and potential time-related biases. …

The Effects Of Overexpressing K2p Channels In Various Tissues On Physiology And Behaviors, Alaina C. Taul, Elizabeth R. Elliott, Douglas A. Harrison, Robin L. Cooper

Biology Faculty Publications

Two-pore-domain K⁺ channels (K2p), known previously as leak channels, are responsible for maintaining the resting membrane potential of cells. Fifteen subtypes are known to exist in humans and eleven are known in Drosophila melanogaster, as well as six subfamilies; however, little is known about the expression of these subtypes in various animal tissues or the impact of altered expression on cellular physiology. The Drosophila melanogaster model allows for selective misexpression of certain neuron subsets, providing insight into individual cell types and the animal’s physiology more generally. Prior research on the overexpression of K2p channels and the resulting behavioral and …

Making Sense Of Myc In Skeletal Muscle, Ronald G. Jones, Sebastian Edman, Nathan Serrano, Yuan Wen, John J. Mccarthy, Christopher S. Fry, Ferdinand Von Walden, Kevin A. Murach

Physiology Faculty Publications

No abstract provided.

Transferrin Receptor 1 Deficiency In Smooth Muscle Cells Attenuates Vascular Remodeling In Hypertensive Mice, Yoshiro Naito, Hisashi Sawada, Tetsuo Horimatsu, Masaki Ohmuraya, Masanori Asakura, Masaharu Ishihara

Physiology Faculty Publications

Transferrin receptor 1 (TfR1), a crucial cellular iron receptor, has a variety of biological functions. We have previously reported that TfR1 abundance increases in the aortic media of hypertensive rat models. However, its role in vascular diseases remains unknown. In the present study, we generated smooth muscle cell (SMC)-specific TfR1 deficient mice and examined the impact of its deletion in mouse models of hypertension induced by angiotensin II or deoxycorticosterone acetate/salt administration. SMC-specific TfR1 deletion attenuated medial thickening and elastin fragmentation in both mouse models of hypertension. These results indicate that TfR1 in SMCs exerts a role in vascular remodeling …

Synthesis Of Novel Derivatives From Machilin C/D As Antiproliferative Agents Against Triple Negative Breast Cancer, Alyson M. Ackerman, Chibuzor Olewele, Bert C. Lynn, Samuel G. Awuah

Chemistry Faculty Publications

Lignans are small polyphenolic compounds that play an active role in plant defense against pathogens and predators. Recently, lignan machilin D was reported to be effective as an anti-tumorigenic agent in triple negative breast cancer (TNBC) tumor-bearing mice. Previous studies have relied on plant-extracted material limiting scalability and diversification of the natural scaffold. Herein, we describe a generalizable one-pot synthesis of machilin D and its derivatives via an iron chloride-induced dimerization of isoeugenol. Employing this synthetic methodology allowed for a robust diversification campaign to access seven (7) new lignan derivatives of the machilin D family with superior anti-proliferative properties in …

Proto-Oncogene Hras Transcript Level And Overall Survival In Stages Ii And Iii Colorectal Cancer, Donghyun Kim, Saima Sharif, Juan Antonio Raygoza Garay, Avanish S. Bhakta, Patrick M. Boland, Michael J. Cavnar, Michelle L. Churchman, Hassan Hatoum, Lyen C. Huang, Joseph Kim, Richard Kim, Robert W. Lentz, Sarbajit Mukherjee, Mary T. O'Donnell, Benjamin Quartey, Matthew J. Reilley, Robert J. Rounbehler, Bodour Salhia, Bryan P. Schneider, Carlos H. Chan

Surgery Faculty Publications

Background: Mutational landscape is prognostic in colorectal cancer (CRC). Rat sarcoma (RAS) oncogenes, such as KRAS and NRAS, with driver mutations, portend poor survival outcomes, whereas pathologic mutations in HRAS are extremely rare, and their prognostic value remains uncertain. Methods: This retrospective study analyzed the Oncology Research Information Exchange Network (ORIEN) alliance tumor RNA-Seq data in Stages II and III CRC to investigate the association between RAS gene expression and survival outcomes. Results: High transcript levels of HRAS were associated with superior overall survival (OS). The high HRAS-associated OS benefit was most pronounced in patients with right-sided primary expressing low …

The Effect Of Cellulolytic Enzyme Preparations As In Vitro Improvement For Forage Digestibility, G Bertin, Ph Hellings

IGC Proceedings (1977-2023)

The systematic study of commercial preparations of fungal cellulases has been undertaken with the aim of determining the principal components and the contribution of each to the digestion of the plant cell wall. These enzymes, normally have three fold role-silage preservative by hydrolysing the cellulosic wall of plant material, cellulases make available fermentable sugars to the lactic bacteria. -Improvement of digestibility we can increase gain by 1 % of the dry matter in forage. - Improvement of intake if the animal digests more quickly his gut will empty more rapidly thus making it available sooner for a new intake of …

Effect Of Cellulase Preparation On Fermentation Of Silage, E No, Y Harasawa, A Ataku, N Narasaki, T Sueyoshi

IGC Proceedings (1977-2023)

The effects of cellulase preparation (Meicelase) on silage fermentation were studied using the first cutting alfalfa and timothy. In one-liter laboratory silos with Meicelase of 0, 0.2, 0.4 and 0:8% of the fresh grasses, the fermentative quality was examined throughout a 30 day ensilage period. The amount of the lactic acid in the silage with the Meicelase was considerably greater than the control silage with no additive. In addition, the pH of that silage was low and the content of water soluble carbohydrate (WSC) was high. However, the differences in the amount of Meicelase applied had no effects in the …

Effects Of Sample Drying Method On Estimates Of Nutritive Value Value, S Uchida, H Hayashi, T Horigome

IGC Proceedings (1977-2023)

The influence of different drying methods for the sample used for analysis on the estimates of the nutritive value of silage was investigated. The silages and hay were made from the same grass and fed to three wethers to determine digestibilities. The samples for analytical use of silages and hay fed, and feces excreted, were prepared by three drying techniques of freeze drying (F), oven drying at 60 C (L) and oven drying at 100 C (H) and then each dried sample was ground in a Wiley mill to pass a 0.5 mm screen. The residual moisture in prepared samples …

Effect Of Lactic Acid Bacteria On Silage Fermentation, Ph Hellings, G Bertin, M Vanbelle

IGC Proceedings (1977-2023)

Inoculants were tested to determine their suitability for use as silage aids. Studies carried out in laboratory indicated (1) that the minimum inoculum should be at least 105 viable organisms per gram of fresh fodder and (2) of all bacterial strains tested, only the Lactobacillus and, specifically, L. casei improved storage characteristics. But the efficiency of an inoculant was subordinated to the fermentable sugars content of the crops. In this respect, alfalfa and second cut grass had the lowest potential for ensilage. Energy was supplied as a mixture of milled barley and amylolytic complex. The influence of a 1: 1: …

Ensilage Of Alfalfa Press-Cake. A Kinetic Model For Ph, Acidity And Nitrogen Evolution, G Amdrich, L Lencioni, R Florentini, G Anelli

IGC Proceedings (1977-2023)

A kinetic model to describe the time evolution of pH, acidity, metabolizable nitrogen and k1 • B, ( a function related to the bacteria concentration) during ensilage of alfalfa press-cake is suggested. The basic hypotheses of the model are given together with the related differential and integral equations. The kinetic model has been tested using experimental data obtained from two samples of alfalfa press-cake respectively ensiled with and without acid addition. The correlation coefficients obtained indicate the reliability of the suggested model.

Ensilage Of Alfalfa Press-Cake, G Anelli, G Andrich, M Contini, L Lencioni

IGC Proceedings (1977-2023)

The Istituto Industrie Agrarie of the University of Pisa has developed a wet green crop fractionation process which involves the ensilage of the press-cake. This note reports some experimental results on the alfalfa press-cake ensiled with or without addition of acid. The evolution of silages was evaluated through organoleptic and chemical parameters. The good behaviour· during the ensilage shows that alfalfa press-cake is suitable to be ensiled even without previous acidification.

Coagulation Of Sheep Milk Added With Alfalfa Juice And Protein Concentrate, L Lencioni, R Florentini, A M. Pisanelli, C Galoppini

IGC Proceedings (1977-2023)

This note reports some experimental results on the preparation of food-grade products obtained by enzyme coagulation of sheep milk added with alfalfa juice, decolourized whole protein concentrate and cytoplasmic protein concentrate. Working conditions are reported together with the yields, chemical composition and organoleptic properties of the coagula. Products obtained from milk supplemented with low alfalfa juice (��5% dry basis) or protein concentrates (;;;;:10% dry basis) show a good acceptability. The ripening greatly reduces grass flavour and taste.

Effect Of Remaining Brown Juice In Wet Protein Curd On The Quality Of Leaf Protein After Drying, Y S. Cho, J K. Kim, T Horigome, S Uchifa

IGC Proceedings (1977-2023)

The influence of remaining brown juice ( deproteinised juice) in wet protein curd on the quality of resulting leaf protein was examined. Freshly cut Italian ryegrass and red clover were fractionated into protein curd, brown juice and pressed cake. Various leaf proteins were prepared by drying the protein curds retaining different amounts of brown juice at 65°C followed by acetone-washing, and by immediate acetone-washing of the protein curd. Casein-brown juice mixtures at the ratio of 1 :3 and 1: 6 were also prepared. The crude protein content of the dry matter in brown juice was very low, 9. 9% for …

The Nutritive Value Of Mixtures Of White Leaf Protein And Other Food Proteins, R Carlsson, P Hanczakowski

IGC Proceedings (1977-2023)

The nutritive value of white leaf proteins were studied, as food supplements. The protein was obtained by heat­fractionation ( Chenopodium quinoa Willd.) or by de-pigmentation (Medicago sativa L. ). The leaf protein was substituting 50% of the protein in a diet, where the remaining 50% came from wheat gluten, soya bean meal, bone-meat meal, fish meal, skim milk or whey. The nutritive value was estimated by the Thomas-Mitchell nitrogen balance method. The proximate chemical composition of the food protein products, and their amino acid composition were analysed. In every mixture but the one with gluten the biological value was higher …

Effect Of Contamination By Aspergillus Flavus On The Nutritive Value Of Leaf Protein Concentrate Of Leucaena Leucocphala During Storage, H T. Ostrowski-Meissner, I Komang, W Supratman, I Barchia, S Cacang

IGC Proceedings (1977-2023)

Leaf protein concentrate extracted from tree legume Leucaena leucocephala cultivar Cunningham (LLP) was stored under humid tropical conditions (24-28°C and 84-92% rh) in the form of wet cake during a 10 day period. Samples of LLP were taken every second day for chemical analyses and protein quality assays. The effect of storage on dried LLP was also assessed against two control proteins: peanut meal (PNM) and fish meal (FM), either free of aflatoxins or infected by the aflatoxigenic fungus Aspergi_llus flavus to the level 280 mcg aflatoxin B1 equivalent/kg. Storage of wet LLP under such conditions resulted in infestation by …