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Manipulating Species Diversity: Environmental Impacts In Row Crop, Livestock, And Grassland Agroecosystems, Alayna A. Jacobs

Theses and Dissertations--Plant and Soil Sciences

The diversity of living species in an agroecosystem affects both natural resources and agricultural production efficiency. The rise of managed plant monocultures has allowed agricultural yields to increase over time. However, simplifying agroecosystems affects the capacity of the system to sustainably provide clean air, water, and productive soils essential for continued food and fiber production. This dissertation manipulates species diversity in row crop and forage agroecosystems prevalent in the Eastern United States and tracks the associated environmental and agricultural production consequences.

In row crop agroecosystems, increasing plant diversity with winter cover crops has been suggested as a strategy to increase …

The Role Of Class Xi Myosin In Fertilization Of Arabidopsis Thaliana, Umma Fatema

Theses and Dissertations--Plant and Soil Sciences

Fertilization is the process of fusion of haploid male and female gametes to develop a new individual. In most animals, microtubules drive the migration of the female pronucleus toward the male pronucleus for fertilization. By contrast, the fertilization process in flowering plants is dependent on actin filament (F-actin) dynamics; F-actin, not microtubules, is responsible for sperm nuclear migration. In one of the female gamete cells, the central cell, constant F-actin meshwork inward movement towards the nucleus takes place to transfer the sperm nucleus for karyogamy. This inward F-actin dynamic is controlled by a unique ARP2/3 (Actin Related Protein 2/3)-independent WAVE/SCAR …

Blocking The Acquisition Of Ethanol-Induced Conditioned Place Preference With 11, 21-Bisphenyl-19-Norpregnane (Pt150) In Coturnix Quail, Mia Radevski

Theses and Dissertations--Psychology

Alcohol use disorder (AUD) has been associated with a dysregulated stress system. Therefore, regulating stress hormones has been investigated as a potential therapeutic target for AUDs. The purpose of the current study was to investigate whether a stress hormone receptor antagonist, PT150, would block the rewarding properties of ethanol. Quail were used as subjects because a conditioned place preference (CPP) apparatus that utilized visual cues was used, and quail readily attend to visual cues. Visual cues in the environment have been shown to become associated with alcohol effects and later induce craving. Starting on day one, quail were pretreated with …

The Role Of Affective Heterogeneity On Treatment Effects For Youth With Conduct Problems, Pevitr Singh Bansal

Theses and Dissertations--Psychology

Conduct problems (CP) are a class of disruptive and aggressive behaviors (e.g., aggression, vandalism) comprised of both oppositional defiant and conduct disorder. CP are highly heterogenous and one vital factor that parses out this heterogeneity is affect, specifically the affective traits of irritability (IRR) and limited prosocial emotions (LPE). The current study examined how IRR and LPE predict distinct aspects of treatment efficacy including (1) treatment response (i.e., magnitude of change from week 1 to week 5); (2) trajectories (i.e., shape of symptom change); and (3) time-out (i.e., behavioral and emotional reactions to time-out). Participants were 49 youth aged 7-12 …

Examining The Indirect Effect Of Trauma On Obsessive-Compulsive Symptoms Through Responsibility/Threat Beliefs, Emily E. Fenlon

Theses and Dissertations--Psychology

Inflated responsibility beliefs and threat estimations have been implicated in the etiology and maintenance of obsessive-compulsive (OC) symptoms. Research suggests that early life experiences that involve actual or perceived serious harm to oneself or others may lead to inflated responsibility/threat beliefs and OC symptoms. Research has yet to explore if traumatic experiences influence responsibility/threat beliefs or if the associations between trauma and OC symptoms are at all dependent on trauma’s influence on responsibility/threat beliefs. The present study aims to examine associations among exposure to potentially traumatic events (PTEs), responsibility/threat beliefs, and OC symptoms. A sample of 886 undergraduate students completed …

Effects Of Snake Fungal Disease On The Survival And Growth Of The Queensnake (Regina Septemvittata), Andrew Jerome Ibach

Theses and Dissertations--Forestry and Natural Resources

Having only emerged as a threat to snakes in 2006, relatively little is known of snake fungal disease’s (SFD) impacts on demographic processes, particularly survival and growth. I used data from an extensive capture-mark-recapture study to examine survival and growth in central Kentucky Queensnake (Regina septemivittata) populations. I found that diseased snakes and healthy snakes possessed similar monthly survival estimates (SFD positive 0.9687, 95% CI 0.8444 to 0.9944; SFD negative 0.8735, 95% CI 0.7518 to 0.9402) and that disease state transition probability from SFD negative to SFD positive, and SFD positive to SFD negative were also similar (N-P …

Nondestructive Multivariate Classification Of Codling Moth Infested Apples Using Machine Learning And Sensor Fusion, Nader Ekramirad

Theses and Dissertations--Biosystems and Agricultural Engineering

Apple is the number one on the list of the most consumed fruits in the United States. The increasing market demand for high quality apples and the need for fast, and effective quality evaluation techniques have prompted research into the development of nondestructive evaluation methods. Codling moth (CM), Cydia pomonella L. (Lepidoptera: Tortricidae), is the most devastating pest of apples. Therefore, this dissertation is focused on the development of nondestructive methods for the detection and classification of CM-infested apples. The objective one in this study was aimed to identify and characterize the source of detectable vibro-acoustic signals coming from CM-infested …

Using Stream Restoration To Mitigate Stormwater Runoff In An Urban Watershed: A Case Study, Jonathan M. Brantley

Theses and Dissertations--Biosystems and Agricultural Engineering

The goal of this case study was to evaluate the effectiveness a 275 m regenerative stream restoration design to mitigate stormwater runoff in a highly urbanized watershed. The restoration resulted in a wide, wetland-like floodplain, comprised of a rock base that was overtopped with a filtration media (approximately 30% woodchips and 70% topsoil). The creation of the floodplain-wetland complex in tandem with filtration media lead to increased storage capacity and an increase in hyporheic exchange within the system. Significant reductions were found for the storm hydrograph parameters volume, peak discharge, and time to peak. Reductions in baseflows were also noted …

Design, Development And Benchmarking Of Machine Learning Algorithms In Biomedical Applications, Qi Sun

Theses and Dissertations--Computer Science

Machine learning algorithms are becoming the most effective methods for knowledge discovery from high dimensional datasets. Machine learning seeks to construct predictive models through the analysis of large-scale heterogeneous data. While machine learning has been widely used in many domains including computer vision, natural language processing, product recommendation, its application in biomedical science for clinical diagnosis and treatment is only emerging. However, the wealthy amount of data in the biomedical domain offers not only challenges but also opportunities for machine learning. In this dissertation, we focus on three biomedical applications from vastly different domains to understand the opportunities and challenges …

We Can Still Feed Ourselves: Food Sovereignty, Aid, Sickness, And Health In Eastern Kentucky, Annie Koempel

Theses and Dissertations--Anthropology

Over forty percent of eastern Kentucky residents are classified as obese. From a biomedical perspective, obesity is linked to a range of chronic diseases, including heart disease, diabetes, and high blood pressure and is caused by particular lifestyle behaviors that lead to an increase in calorie consumption and decrease in calorie expenditure. However, these links – individual behavior leads to obesity which leads to chronic disease - do not take into account a wide range of personal, social, environmental, political, and economic conditions. In addition to the assumptions of what it means to become and be obese, Kentucky is regularly …

An Investigation Of Hhv6'S Impact On The Cognitive Progression And Microglial Changes In An Alzheimer's Disease Cohort, Charles E. Seaks

Theses and Dissertations--Physiology

The role of herpesviruses and, more specifically, HHV6 in the development of Alzheimer’s disease (AD) and associated cognitive decline is still being investigated. High ubiquity and prevalence in the population have led to a high degree of skepticism about HHV6 as a potential contributor to cognitive decline and dementias. However, recent evidence related to another herpesvirus, herpes simplex virus 1, suggests that reactivation, not carriage, of the virus may be the key factor to explain the dissonance between the virus’ ubiquity and contributions to dementias. With that in mind, we set out to assess cases from the Sanders-Brown Center on …

The Impact Of Korean Government’S Industrial R&D Expenditure On Technological Competitiveness, Hyunok Kim

MPA/MPP/MPFM Capstone Projects

In Korea, the total amount of R&D executed by the Korean government in 2019 was $16 billion. The Compound Annual Growth Rate of Korean government R&D budget is 5.04% (2010~2019). This paper studies whether the Korean government's R&D expenditure effectively achieves its purpose. Assuming the purpose of government R&D expenditure is set as science and technology competitiveness based on related laws and the indicator is identified as the technology balance of payments (TBP), this study estimates the statistical correlation between government R&D expenditure and TBP. Since the characteristic of government R&D expenditure is different for each field, the correlation by …

The Hybrid Teacher: Expanding The Vocal Pedagogy Regime, Mary Joy Nelson

Theses and Dissertations--Music

The world of professional vocal training is becoming more and more eclectic and demanding in the United States. Academic music programs, and subsequently, vocal and vocal pedagogy programs are still predominantly classical in 2022, due to the American university system’s historical roots. In the 21st century, the commercial music industry is at an all-time high. However, in contemporary academia, vocal and vocal pedagogy degree programs remain primarily classical. In recent years, voice professionals have been discussing the implications of academic programs updating their programs and pedagogy in order to include professional CCM and hybrid training. Classical, CCM and hybrid singers …

Career Development And Employability Skill Integration Within The Equine Science And Management Undergraduate Program At The University Of Kentucky, Savannah Faye Robin

Theses and Dissertations--Educational Leadership Studies

College graduates need to be equipped with career and employability skills that are necessary to be meaningfully and gainfully employed in their future. These skills consist of self-management, career-management, essential-employability, and discipline-specific skills. More responsibility is being placed on college and university academic programs to ensure that students are graduating with these skills when they enter the workforce. However, students are growing less likely to participate in out of course career development activities and resources. Integrating these skills into program curriculum can be an effective way to ensure that all students have the ability to develop these necessary skills.

While …

Housewives To Heroines: Continuing Education For Women At The University Of Kentucky, 1964-1988, Allison L. Elliott

Theses and Dissertations--Educational Policy Studies and Evaluation

Beginning in the early 1960s, the movement for the continuing education for women (CEW) brought together a seemingly unlikely alliance of American activists, educators, philanthropists, and government agencies. Fueled by philanthropic funds, accelerated by the quest for “womanpower” to bolster national defense, and aligned with regional workforce needs as well as the personal goals of individual women, CEW programs pioneered new models of academic advising and student support that continue to influence higher education practitioners today. By studying the experiences of both administrators and students involved with CEW at the University of Kentucky, this study sheds light on how one …

How Does Exposure To An Authentic English-Speaking Environment Contribute To Chinese International Students’ Academic Reading Comprehension?, Na Liu

Theses and Dissertations--Education Sciences

Chinese international students comprise the largest group of international students in U.S. universities. To better support international students to be successful in international higher education, Chinese students’ reading comprehension in English deserves attention. Using a qualitative research design, this study analyzed Chinese international students’ perceptions about their English reading comprehension in academic literacy, and how exposure to an English-speaking environment contributed to their reading comprehension development. There were 8 participants involved who have different genders (males and females), education levels (undergraduates and graduates), exposure time (less than one year and more than one year), and majors (chemistry, engineering, arts, etc.). …

Development Of The Elementary Teachers’ Commitment To Outdoor Education Survey: A Mixed Methods Study, Sagan Goodpaster

Theses and Dissertations--Education Sciences

As the Green Schoolyards Movement continues to grow throughout North America, outdoor education (OE) is gaining more attention in the formal K-12 curriculum (Dyment, 2005; Sterrett & Imig, 2015). Rooted in the pedagogies of place-based and experiential learning, outdoor instruction provides social, psychological, physical, and academic benefits for students, and fosters their sense of connection to the natural environment (Dubel & Sobel, 2008; Robertson, 2017). In particular, outdoor learning opportunities have been shown to promote greater gains in students’ scientific content knowledge (Carrier et al., 2014; Cronin-Jones, 2000; Dirks & Orvis, 2005; Kenney et al., 2003). Outdoor instruction involves students …

Knitting Code: Examining The Relationship Between Knitting And Computational Thinking Skills Using The Nexus Of Practice, Emily Rose Dodson-Snowden

Theses and Dissertations--Education Sciences

Due to the rise of careers in STEM-related fields, there is a growing need for schools to produce people to fill these positions. One area of STEM that is growing is computer science/coding. Due to this demand, schools need to be intentional about exposing students to computer science/coding. There are a variety of new tools to introduce students to this field. One growing belief is that knitting can teach computer science/coding to students.

The goal of this study was to see if knitting can serve as an introduction to teach students computation skills. Kitting has historically been used to code …

Momentary Endurance: The Effects Of Mouthpiece Pressure And Capillary Refill Time On Short-Term Muscle Recovery In Trumpet Players, Taylor Gustad

Theses and Dissertations--Music

The topic of proper mouthpiece pressure usage has been widely pushed off to the margins of trumpet pedagogy, only receiving the passing mention in a limited number of method books. This research paper contains a cross sectional view of the few, but most prominently used method books in the trumpet community that shows the prevalence of this issue, as well as steps to bring in new and useful information in this field. There are several aspects of mouthpiece pressure that should be studied; however, this paper focuses on its connection to momentary endurance and capillary refill time.

Momentary endurance is …

Patient And Therapist Perceptions Of Respiratory Functioning Due To Spinal Cord Injury: Implications For Music Therapists, A'Marie Dotson

Theses and Dissertations--Music

Decreased respiratory functioning is a common symptom of spinal cord injury (SCI) and can impact individuals’ personal, professional, and social lives. In spite of this, researchers have not explored the use of music in the lives of individuals with SCIs or music’s role in their healthcare professionals’ treatment plans to improve respiratory functioning. The purpose of this qualitative study was to better understand how decreased respiratory functioning affects individuals with SCIs and healthcare professionals through a multiple case study design. Participants were two individuals with SCIs and three healthcare professionals (physical therapist, recreational therapist, and music therapist) who worked with …

An Agronomic-Economic Approach To Connect Manure Nutrients Back To Grain-Producing Regions, Joshua M. Duke, Joshua M. Mcgrath

Plant and Soil Sciences Faculty Publications

Stakeholders for nutrient-impaired watersheds have long discussed the causes and consequences of nutrient surpluses associated with intensive livestock production. Nonetheless, nutrient surpluses relative to crop requirement, particularly with phosphorus (P), persist and continue to contribute to water quality impairment. Nutrient life-cycle analysis shows that mineral P, from soil minerals or mined fertilizer P, flows to livestock regions from grain-producing regions. Although creating a return flow of these nutrients to grain fields seems like an easy solution, significant economic obstacles exist to creating a connected manureshed over large geographic distances. To limit the impact of manure use on local surface water, …

Social And Biological Determinants Of Pregnancy-Related Mortality And Morbidity In A Rural, Underserved Population, Anna Hansen

Theses and Dissertations--Sociology

Cases of severe maternal morbidity (SMM) and pregnancy-related mortality (PRM) are increasing in the US. Research concerning SMM and PRM has neglected women in Central Appalachia; a largely rural, health-disparate population. The aims of this study are two-fold: (1) Examine patient-level and place-based predictors of SMM/PRM via hierarchical logistic regression modeling, and (2) Elucidate Appalachian healthcare patients’ and providers’ experiences with SMM/PRM, perceptions of contributing factors, and insights on points of intervention.

This study uses a mixed methods approach guided by the WHO’s conceptual framework for action on social determinants of health to identify determinants of SMM and PRM among …

An Examination Of Three Transitional Events In The Substance Misuse Trajectories Of Women With Criminal Legal System Involvement, Martha Tillson

Theses and Dissertations--Sociology

Research has consistently demonstrated that criminal legal system (CLS)-involved women are distinct from men in initiation and course of drug use, with important differences on biological, environmental, and sociocultural levels. Thus, the unique pathways and transitions into and out of drug use for women with CLS involvement are critical to consider from a research perspective, but also from a need to develop and support evidence-based, women-centered services in correctional contexts. This dissertation project uses a three-paper format to investigate three aims: (1) to understand CLS-involved women’s initiations to injection drug use and their experiences providing injection initiation assistance (IIA) to …

The Role Of Shoc2 In Embryonic Development, Rebecca G. Norcross

Theses and Dissertations--Molecular and Cellular Biochemistry

The canonical ERK1/2 signaling cascade regulates cellular functions critical in vertebrate embryonic development such as proliferation, apoptosis, differentiation, and migration. Thus, its signals are controlled by a variety of mechanisms. Scaffold proteins are considered central to the mechanisms regulating the transmission of the ERK1/2 signals. Yet, their functions in development nor the molecular mechanisms by which they exert their control are not well understood.

This study focuses on the essential regulator of ERK1/2 signals during development – the scaffold protein Shoc2. Loss of Shoc2 leads to early embryonic lethality in mice and zebrafish. Germline mutations in the shoc2 gene result …

Structural Basis For Reversible Glucan Phosphorylation By Glucan Dikinases And Glucan Phosphatases, Tiantian Chen

Theses and Dissertations--Molecular and Cellular Biochemistry

Plants store glucose in the form of starch, an insoluble macromolecule that is synthesized and degraded diurnally. Glucan phosphatases play a critical role in starch breakdown, working with glucan dikinases in a cyclic phosphorylation and dephosphorylation. Herein, we present a novel glucan phosphatase Like SEX Four3 (LSF3) from Physcomitrella patens. Structural and enzymatic studies demonstrate that LSF3 is a dimeric glucan phosphatase that displays robust specific glucan phosphatase activity. Dimerization is shown to be essential for LSF3 stability and function. Structure-guided bioinformatic analysis shows that LSF3 is found in early land plants and green algae.

Building upon our understanding of …

An Integrated Computational Pipeline To Construct Patient-Specific Cancer Models, Daniel Plaugher

Theses and Dissertations--Mathematics

Precision oncology largely involves tumor genomics to guide therapy protocols. Yet, it is well known that many commonly mutated genes cannot be easily targeted. Even when genes can be targeted, resistance to therapy is quite common. A rising field with promising results is that of mathematical biology, where in silico models are often used for the discovery of general principles and novel hypotheses that can guide the development of new treatments. A major goal is that eventually in silico models will accurately predict clinically relevant endpoints and find optimal control interventions to stop (or reverse) disease progression. Thus, it is …

What Is The Sound Of Holiness? The Regulation Of Sound And Silence In Early Medieval Monastic Communities, 600-900, Melissa Kapitan

Theses and Dissertations--History

This dissertation explores how the regulation of sound and silence in the early medieval monastery formed a distinct soundscape imbued with personal, communal, and theological resonances. More narrowly, this investigation focuses on the Frankish empire of the ninth century, which saw a series of monastic reforms led by the Carolingian royal family and ecclesiastical figures and consolidated in imperial legislation and treatises on the monastic life.

These ninth-century reforms witnessed not only the promotion of a single rule and custom to guide monastic life, but also a great flowering of liturgical texts, including the earliest treatises on musical theory. These …

Deletion Of Period Genes Exacerbates Diet-Induced Obesity In Female, But Not Male, Mice, Elizabeth Kantra

Theses and Dissertations--Biology

Sex differences in obesity in mice are mediated by differential responses of circadian rhythms to high-fat feeding in males and females. Other studies also showed circadian Period genes regulate diet-induced obesity in mice. In this study, we investigated the role of the Period genes in regulating sex differences in obesity. Male and female C57BL/6J wild type, Per1/2 KO, and Per1/2/3 KO mice were housed in 12L:12D and fed high-fat diet for 12 weeks. We found a striking sex difference in obesity such that disabling the Period genes exacerbated adiposity in female, but not male, mice. Increased adiposity in female Period …

Understanding The Effects Of Embryonic Hyperglycemia On Retinal Development And Maintenance, Kayla Titialii-Torres

Theses and Dissertations--Biology

Hundreds of millions of people are affected by diabetes worldwide. Whether they are diagnosed with prediabetes or Type I or II diabetes, there are a variety of mechanisms in the pathogenesis of diabetes. Diabetes is a disease which consists of recurring states of hyperglycemia that can be difficult to manage due to either lack of insulin production or improper utilization of insulin. While these mechanisms of action differ, complications induced by diabetes occur in both poorly regulated Type I and II. Common complications of diabetes include nerve damage, kidney damage, and eye damage. Eye damage specifically is called diabetic retinopathy …

Identifying Epidermal Enriched Genes Required For Planarian Regeneration- Sp. Schmidtea Mediterranea, Pallob Barai

Theses and Dissertations--Biology

The outer epithelial layer covering an organism, commonly known as the epidermis, is crucial for maintaining homeostasis and for the wound healing processes after injury. The planarian epidermis allows flatworms to heal their wounds and virtually restore any missing tissues. Immediately after amputation, planarians contract their muscle and stretch their epidermis to heal the wound area. However, how the planarian epidermis coordinates with other tissues and mechanisms after the initial wound healing processes begins is not understood in detail. I hypothesized that epidermal cell stretching upon wound healing induces transcriptional changes that are required for effective regeneration. To test this …