Digital Commons Network^™

Cause Or Consequence? Evaluating The Evidence For Housing Instability As A Predictor Of Adult Well-Being, Hope Harvey, Brielle Bryan

Sociology Faculty Publications

No abstract provided.

Antibiotic Alternatives And Next-Generation Therapeutics For Salmonella Control: A One Health Approach To Combating Antimicrobial Resistance, Mohamed Saleh, Ashutosh Verma, Khaled A. Shaaban, Yosra A. Helmy

Veterinary Science Faculty Publications

The growing prevalence of antimicrobial resistance has significantly compromised the efficacy of conventional antibiotic-based interventions in controlling Salmonella infections across human and veterinary settings. This growing challenge necessitates a strategic rethinking of pathogen control, prompting the integration of next-generation therapeutics capable of disrupting Salmonella pathogenesis through novel, antibiotic-sparing mechanisms. In this context, a diverse array of emerging alternatives, including bacteriophages, antimicrobial peptides, probiotics, prebiotics, short-chain fatty acids, nanoparticles, and host-directed immunomodulators, have gained prominence as a promising frontier in non-antibiotic therapeutics. These modalities offer targeted approaches to inhibit Salmonella colonization, virulence expression, and persistence, while minimizing collateral damage to the …

A Large Electroencephalogram Database Of Freewill Reaching And Grasping Tasks For Brain Machine Interfaces, Bhoj Raj Thapa, John Boggess, Jihye Bae

Electrical and Computer Engineering Faculty Publications

Brain machine interfaces (BMIs) offer great potential to improve the quality of life for individuals with neurological disorders or severe motor impairments. Among various neural recording modalities, electroencephalogram (EEG) is particularly favorable for BMIs due to its noninvasive nature, portability, and high temporal resolution. Existing EEG datasets for BMIs are often limited to experimental settings that fail to address subjects’ freewill in decision making. We present a large EEG dataset, containing a total of 6808 trials, recorded from 23 healthy young adults (eight females and 15 males with an age range from 18 to 24 years) while performing reaching and …

Addressing The "Hidden Crisis" In Doctoral Education: Identifying The (De)Motivating Effects Of Memorable Messages From A Self-Determination Theory Perspective, Alexis A. Murphy

Theses and Dissertations--Communication

Although PhD students are academically capable, they paradoxically produce alarmingly high attrition rates. For several years, retention rates in PhD programs have averaged between 40% and 70% (Ames et al., 2018; Bowen & Rudenstine, 1992; Cochran et al., 2014; Gardner & GoPaul, 2012; Lovitts, 2001; Rockinson-Szapkiw & Spaulding, 2012). Scholars have presented several potential reasons that PhD students discontinue their studies, none of which include academic ability (Lovitts, 2001; Tucker, 1964; Sverdlick et al., 2018). Framed by self-determination theory (SDT), this study explores the social and communicative components of the doctoral education system by the psychological impact of memorable messages …

Virtual Speeches, Real Fears: Public Speaking Anxiety In The Online Communication Classroom, Timothy J. Bill

Theses and Dissertations--Communication

While universities have explored online course offerings for decades, the COVID-19 pandemic accelerated this trend. The foundational communication course—a course that continues to traditionally emphasize public speaking—has felt the pandemic influence to increase opportunities for students to learn public speaking via online instruction. While scholars have learned much about the student experience of public speaking anxiety (PSA) in face-to-face settings, less is known about their experience of PSA in online courses. This dissertation explored the fears students experience when presenting online and how they view the online presentation environment.

Using Bandura’s Social Cognitive Theory as a guide, this dissertation employed …

The Information Practices Of Undergraduates Encountering Material Objects As Information Resources In Classroom Learning Tasks, Christopher A. Miller

Theses and Dissertations--Communication

Undergraduate students sometimes encounter material objects as information resources in small-group classroom learning activities. Examples include art, artifacts, specimens, and anatomical models. This dissertation sought to identify common features of the students’ information practices in such situations, regardless of the activity’s discipline or content, and to explore how making material information central shapes students’ information practices. The conceptual framework combined theorizations of materiality and information practices. Methods involved video recording students doing a variety of simulated classroom activities with objects, video elicitation, qualitative content analysis, and praxiographic interpretation. The praxiographic interpretive descriptions illustrated how all aspects of the site–material, bodily, …

Crystal Structures And Low-Affinity Complex Formation Of Halogenase Ctcp And Fad Reductase Ctcq From The Chlortetracycline Biosynthetic Pathway, Caixia Hou, Sylvie Garneau-Tsodikova, Oleg V. Tsodikov

Pharmaceutical Sciences Faculty Publications

Enzymatic halogenation in natural products has been intensely investigated due to its potential utility as a tool to improve pharmacological and pharmaceutical properties of drug leads. Chlortetracycline (CTC), the first tetracycline (TC) antibiotic discovered nearly eight decades ago, contains a chlorine group. This chlorine is installed enzymatically by the flavin adenine dinucleotide (FAD)-dependent halogenase CtcP. CtcP and the FAD reductase CtcQ, which is also encoded in the CTC biosynthetic gene cluster, function as a two-component system. Structural information on CtcP and CtcQ has been lacking. In this study, we determined crystal structures of CtcP from Kitasatospora aureofaciens in a complex …

A Cocaine Hydrolase Engineered From Human Butyrylcholinesterase As A Medical Countermeasure For Aldicarb Poisoning, Johnathan E. Lesaint, Nellore Bhanu Chandar, Annet Kyomuhangi, Huimei Wei, Fang Zheng, Chang-Guo Zhan

Pharmaceutical Sciences Faculty Publications

Aldicarb is known to be the most toxic carbamate pesticide and could be used easily by terrorists to cause a mass casualty incident. It is highly desired to discover a medical countermeasure for aldicarb poisoning. Based on structural insights from molecular modeling and in vitro experimental validation, CocH3-Fc(M3), a potent cocaine hydrolase engineered from human butyrylcholinesterase (BChE), has a ~4-fold improved binding affinity with aldicarb compared to wild-type human acetylcholinesterase (AChE) and BChE and a 9-28-fold improved bimolecular rate constant for the carbamylation with aldicarb compared to AChE. CocH3-Fc(M3) also has significant catalytic activity for aldicarb hydrolysis and, hence, is …

Development Of A Ghrelin Deacylase To Attenuate Drug Reward And Associated Effects Of Methamphetamine, Madeline J. Stewart, Huimei Wei, Nellore Bhanu Chandar, Fang Zheng, Chang-Guo Zhan

Pharmaceutical Sciences Faculty Publications

All addictive substances directly or indirectly interact with the dopamine reward system to alter the brain’s dopamine receptor activities. It is essential for a truly effective addiction medication to attenuate substance reward and normalize the brain’s physiological functions. Conventional pharmacological intervention approaches to the treatment of substance addiction usually aim to develop and deliver a potential therapeutic agent to the brain to directly block or decrease actions of the substance or its therapeutic target in the brain. However, it is a grand challenge to attenuate the substance reward without affecting the normal physiological functions of brain receptors or transporters. Here, …

Effects Of Biased Analogues Of The Kappa Opioid Receptor Agonist, U50,488, In Preclinical Models Of Pain And Side Effects, Ross Van De Wetering, Loan Y. Vu, Lindsay D. Kornberger, Dan Luo, Brittany Scouller, Sheein Hong, Kelly F. Paton, Thomas E. Prisinzano, Bronwyn M. Kivell

Pharmaceutical Sciences Faculty Publications

Kappa opioid receptor (KOR) agonists have well-established antinociceptive effects. However, many KOR agonists have negative side effects, which limit their therapeutic potential. Some researchers have suggested that the development of biased agonists that preferentially stimulate KOR G-protein pathways over β-arrestin pathways may yield drugs with fewer adverse side effects. This was investigated in the current study. We describe the synthesis and characterization of three U50,488 analogues, 1, 2, and 3. We evaluated the acute and chronic antinociceptive effects of these compounds in mice using the warm-water tail flick assay and in a paclitaxel-induced neuropathic pain model. Side …

Effects Of Fentanyl-Adulterated Methamphetamine On Circulating Ghrelin In Rats, Huimei Wei, Elise C. Maul, Shawn Park, Kaniz Fatema, Daniel J. Peter, Chang-Guo Zhan, Fang Zheng

Pharmaceutical Sciences Faculty Publications

The appetite hormone ghrelin influences biological processes that are responsible for substance use disorder, which is related to alcohol and most abused drugs including cocaine, methamphetamine, nicotine, etc. In general, upregulation of the ghrelin system enhances drug cravings and substance use. Studies reported in the literature consistently demonstrated that the ghrelin system is associated with stimulants. However, research on opioids in combination with methamphetamine has not been reported. In this study, we examined the relationship of circulating ghrelin with the polydrug use of fentanyl and methamphetamine in male Sprague-Dawley rats, demonstrating for the first time that concurrent use of fentanyl …

Fluorescence-Guided Resection Of Gl261 Red-Fluc And Trp-Mcherry-Fluc Mouse Glioblastoma Tumors, Louis T. Rodgers, Bryan J. Maloney, Anika M. S. Hartz, Björn Bauer

Pharmaceutical Sciences Faculty Publications

Background: Most preclinical studies on glioblastoma (GBM) fail to provide translational utility in the clinic. Fluorescence-guided surgery using 5-aminolevulinic acid (5-ALA) improves tumor resection, disease prognosis, and, thus, patient outcomes. Given the critical role of surgery in managing recurrent GBM, it is essential to incorporate surgical elements into preclinical models to accurately reflect clinical scenarios and enhance translational success. However, existing protocols for 5-ALA-guided resection in preclinical models are limited and often lack clinical relevance.

Methods: To address this gap, we developed a novel protocol for the 5-ALA-guided resection in two mouse GBM models: TRP-mCherry-FLuc and GL261 Red-FLuc.

Results: The …

Development Of Zafirlukast Analogues For Improved Antithrombotic Activity Through Thiol Isomerase Inhibition, Justine A. Keovilay, Kaitlind C. Howard, Kirk A. Taylor, Sabeeya Khan, Sienna E. Wurl, Melanie K. Szahaj, Tanya Sage, Nishad Thamban Chandrika, Caixia Hou, Oleg V. Tsodikov, Jonathan M. Gibbins, Sylvie Garneau-Tsodikova, Daniel R. Kennedy

Pharmaceutical Sciences Faculty Publications

Background:   Thiol isomerases play essential and nonredundant roles in platelet activation, aggregation, and thrombus formation. Thiol isomerase inhibitors have the potential to overcome the 2 major drawbacks of current antithrombotic therapies, as they target both arterial and venous thrombosis without enhancing bleeding risks. Recently, a Food and Drug Administration–approved drug, zafirlukast (ZAF), was shown to be a promising pan-thiol isomerase inhibitor. The objective of this study is to develop analogues of ZAF with optimized thiol isomerase inhibition and antithrombotic activity.   Methods:   Thirty-five ZAF analogues were tested in an insulin turbidometric assay for thiol isomerase inhibition. Analogues were tested for platelet …

Functional And Structural Studies On The Esperamicin Thioesterase And Progress Toward Understanding Enediyne Core Biosynthesis, Erome D. Hankore, Mitchell D. Miller, Abigael J. Kosgei, Weijun Xu, Kemin Tan, Michael Endres, Minakshi Bhardwaj, Grazyna Joachimiak, Andrzej Joachimiak, George N. Phillips Jr., Jon S. Thorson, Steven G. Van Lanen

Pharmaceutical Sciences Faculty Publications

Enediynes are among the most potent antitumor and antibacterial natural products. Studies on their biosynthetic pathways have identified a shared, linear polyene precursor generated from an iterative type I polyketide synthase (PKSE) as the source of the enediyne warhead. A key step is the release of this polyene from the PKSE by a discrete thioesterase (TE). Here, we used X-ray crystallography, site-directed mutagenesis, and heterologous coexpression of PKSEs and TEs to elucidate how enediyne TEs mediate the production of the polyene. We solved the structure of wild-type EspE7 from esperamicin producer Actinomodura verrucosospora. The substrate binding pocket was also …

Sonic Photovoice And Critical Literacy: Listening To And Sounding What Is Me, What Is Not Me, And What Is Between, Alexander Chadwell

Theses and Dissertations--Curriculum and Instruction

This study examined sonic photovoice, a sound-based arts research method, as a tool for developing critical literacy in middle and high school students. Participants found and recorded sounds related to three categories, “what is me,” “what is not me,” and “what is between,” composed a sound collage using the sounds, and reflected on the experience. Framed by 4EA cognition, participatory sensemaking, and relational and everyday aesthetics, the study explored how listening and sounding shaped students’ conceptions of self and reality. Data from recordings, journals, and interviews suggest that the sonic engagement can foster relational, emergent understandings of self and …

Regulation Of Human Dna Mismatch Repair, Breanna Knicely

Theses and Dissertations--Toxicology and Cancer Biology

The DNA mismatch repair (MMR) pathway identifies and corrects misincorporations that arise during DNA replication. MMR is a pathway that is critical for maintaining genomic stability. Mutations within the MMR pathway, both germline and somatic, lead to the development of cancer. There are a variety of mechanisms that regulate the MMR pathway including protein-protein interactions, DNA damage response, and post-translational modifications. In this study, we aim to dive deeper into each of these mechanisms to obtain novel information regarding the mechanisms and regulation of human DNA MMR. In this study we generated hExo1 constructs mutating Exo1’s interactions with MSH2 and …

The Paradox Of Survival: Gendered Food Insecurity, Labor Inequalities, And Coping Strategies During The 2023/4 Drought In Eastern Ethiopia, Meron E. Lemma

Theses and Dissertations--Geography

In drought-prone regions of Eastern Ethiopia, recurring droughts profoundly reshape everyday life in ways that deepen existing gendered inequalities. Drawing on fieldwork conducted in Fedis district, including semi-structured interviews with women, project implementers, and government officials, as well as group discussions with community members, this study explores how women experience, navigate, and resist the gendered impacts of drought. By attending to the sociopolitical dynamics of drought and the development interventions that accompany it, I analyze how these forces restructure labor, land, food systems, and authority in ways that reproduce and exacerbate existing inequalities. Women’s coping strategies, ranging from begging and …

Commercial Attention Spaces: A Century Of Discourses, Measurements, And Models Of Attention In The Construction Of U.S. Advertising Markets, Jacob Saindon

Theses and Dissertations--Geography

A vast assemblage of networked devices populate our everyday spaces. Concomitant with the increasing ubiquity of media devices are advertisements. Indeed, many networked devices, specifically the “free-to-use” software applications, websites, or platforms displayed through them, are advertising-dependent. An assortment of firms within the commercial media industry—brands, agencies, publishers, vendors—work in concert to sustain a market for advertising. The core operation of the commercial media industry, I argue, is the production and exchange of attention as an economic object. In this study, I approach the production of attention and the framing of its markets as spatial processes. The continued growth of …

Igc Oral Presentation Summary: Xxv Igc Congress, S. Ray Smith, Echo Elizabeth Gotsick, J. R. Weinert-Nelson, Alayna Jacobs, Christopher D. Teutsch, Will Fleming, D. R. Woodfield, James L. Klotz, Jeff Lehmkuhler, Greg S. Halich, Brittany Davis, Brittany Hendrix, Krista Lea

IGC Proceedings (1977-2023)

This paper will give an overview of the XXV International Grassland Congress thematic, keynote, and plenary sessions. The theme of the XXV IGC Congress meeting was “Grasslands for Soil, Animal, and Human Health.” We had many people attending, presenting, and participating in all these sessions. Within a month after the conclusion of the Congress, the proceeding’s papers presented at the meeting will be available on the IGC app. By the end of 2023 all papers will be available for download through any search engine at internationalgrasslands.org.

Eco-Friendly Synthesis And Stability Analysis Of Cspbbr3 And Poly(Methyl Methacrylate)-Cspbbr3 Films, You-Lin Huang, Wei Li, Fuqian Yang

Mechanical Engineering Faculty Publications

This study presents an eco-friendly mechanochemical synthesis of cesium lead bromide (CsPbBr₃), eliminating the need of organic solvents and high temperatures. The synthesized CsPbBr₃ powder is used to fabricate poly(methyl methacrylate) (PMMA)-CsPbBr₃ films and CsPbBr₃ nanocrystals (NCs). The photoluminescence (PL) peaks of the emission light are centered at 541 nm, 538 nm, and 514 nm for the CsPbBr₃ powder, PMMA-CsPbBr₃ films, and CsPbBr₃ NCs, respectively, correlating with crystal sizes of 0.96, 0.56, and 0.12 μm, respectively. The PL lifetime analysis reveals decay times () of (4.18, 20.08), (5.7, 46.99), and (5.81, …

Investigating Non-Functional Overreaching In Special Operations Tactical Training, Jeremy Alan Ross

Theses and Dissertations--Rehabilitation Sciences

Special Operations Forces (SOF) tactical training requires high levels of physical performance and psychological resilience. This training is often characterized by extreme temperatures and environments, extremely high levels of physical activity and large caloric deficits. As such, SOF personnel follow intense strength and conditioning (S&C) programs to enhance a wide range of athletic abilities. Such programs rely on a balance between training stress and recovery to allow for gradual improvement in physical ability over time, however it is unknown how to quantify tactical training stress in the context of a training program. This dissertation explores evidence related to how this …

Knee Health Is A Major Determinant Of Mobility Across The Healthspan, Brandon M. Peoples, Kenneth Harrison, Michael A. Samaan, Christopher B. Mobley, David T. Redden, Jaimie A. Roper

Kinesiology and Health Promotion Faculty Publications

Knee health constitutes a pivotal determinant of locomotor function and overall mobility throughout the healthspan. Impairments in knee function lead to a series of biomechanical and physiological alterations that pose substantial public health challenges and economic burdens worldwide. This review synthesizes evidence that delineates the complex and multifaceted relationship between knee health and mobility, emphasizing the interplay across various domains in adult populations. Knee health is conceptualized as a dynamic, interconnected system whereby structural integrity, neuromuscular mechanisms, biomechanical adaptations, and functional mobility are intricately interrelated through cascades of mechanistic processes operating across different temporal stages. A comprehensive understanding of these …

Adapting A Mobile Health Intervention To Improve Prep Adherence Among Young Black Msm In Fulton County, Georgia, Kassidy Maust

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Young Black men who have sex with men (YBMSM) in the southern United States, particularly in Fulton County, Georgia, face disproportionately high rates of HIV and unique structural barriers to pre-exposure prophylaxis (PrEP) uptake and adherence. Sustained adherence among YBMSM remains low due to intersecting structural, social, and behavioral barriers. To address these disparities, this paper presents an adapted implementation of the evidence-based M-Cubed (Mobile Messaging for Men) mHealth intervention. Prior studies of M-Cubed and similar mHealth interventions have shown promising outcomes in increasing PrEP adherence among Black MSM populations. The adapted intervention integrates culturally tailored messaging specific to behaviors …

Air Pollution Exposures And Cognitive Function Among Older Americans, Clarisse Nyondo Okoma

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Background: New findings indicate that environmental factors, such as air pollution, could contribute to cognitive decline among older adults. Nonetheless, extensive longitudinal studies in the U.S. investigating this connection are few and far between.

Objective: This study assessed the relationship between long-term exposure to ambient air pollutants and cognitive function in older adults in the United States.

Methods: We examined data from the Health and Retirement Study (2010-2018), which included 234,250 observations from adults aged 50 and older. Cognitive performance was evaluated based on immediate and delayed word recall, a 27-point cognitive summary score, backward from 20, and …

Demonstration Of An Enhanced Dosing Pattern For Debulking Large And Bulky Unresectable Tumors Via Differential Hole-Size Spatially Fractionated Radiotherapy, Joshua Misa, William St. Clair, Damodar Pokhrel

Radiation Medicine Faculty Publications

Purpose/objective

We propose a novel lattice deployment for spatially fractionated radiotherapy (SFRT) treatments. In this approach, a larger diameter high-dose sphere is centrally placed in the bulky tumor mass and surrounded by smaller diameter high-dose spheres.

Materials/methods

Thirty SFRT patients (10 head and neck [HN], 10 abdominal/pelvis, and 10 chest/lung cases) treated with an MLC-based crossfire method were retrospectively analyzed. Eleven differential hole-size lattice patterns were benchmarked against the clinically delivered SFRT plans (1 cm diameter cylinders, 2 cm spacing) and the standard uniform lattice pattern (1.5 cm diameter spheres, 3 cm spacing). These patterns varied in core diameter (C: …

Black Women, Bold Science: Navigating Race, Gender, And Identity In Science, Mia L. Brown

Theses and Dissertations--Science, Technology, Engineering, and Mathematics (STEM) Education

This research study examines the challenges Black women encounter when in predominantly white, male academic and professional science settings, where they experience dual discrimination because of their race and gender. These biases perpetuate the negative stereotype that Black women are less competent than white men. This mixed methods study examines how Black women navigate their race, gender, and science identities within predominantly white institutions through their doctoral programs and professional science careers. The study implements Critical Race Mixed Methodology to combine survey data for a wide reach with interview data for detailed exploration. The study involved eight Black women science …

Navigating Stress In Higher Education Leadership: The Role Of Mindful Meditation For Women Administrators, Christine T. Harper

Theses and Dissertations--Educational Leadership Studies

Women higher education administrators encounter distinct stressors which can significantly impact wellbeing and lead to burnout. These challenges include implicit bias, difficulty achieving work-life balance, and increasing institutional expectations. This study utilizes a Mixed Methods Action Research (MMAR) framework to assess stress levels, trait mindfulness, coping mechanisms, and the effectiveness of a structured mindfulness-based intervention. While the quantitative analysis revealed only a slight increase in trait mindfulness and no notable reduction in stress levels, the qualitative findings showcased meaningful personal benefits to the participants. They reported heightened awareness of stress triggers, improved emotion regulation, and an ability to apply the …

Magnetic Heating Efficiency Of Magnetite Nanoparticles: Dependence On Size And Alternating Magnetic Field, Sarah E. Kubican

Theses and Dissertations--Biomedical Engineering

Magnetic heating mediated by magnetic iron oxide nanoparticles (MION) has several clinical applications including cancer thermal therapy, tissue thawing for organ preservation, and magnetogenetics. However, current models of magnetic heating, including the widely used linear response theory, inadequately predict MION heating efficiency. An experimental study covering alternating magnetic field (AMF) strengths and frequencies ranging from 3.98 – 27.85 kA/m and 103.6 – 984.1 kHz, respectively, with a range of MION core diameters 9.5- 30.3 nm was conducted to elucidate the relationships between these AMF parameters and MION sizes and the resulting heating efficiencies. Heating efficiency was found to increase approximately …

Pathogen Surveillance Of Viruses And Antimicrobial Resistance Through Wastewater Based Epidemiology, William Strike

Theses and Dissertations--Biomedical Engineering

Pandemic surveillance is a cornerstone in public health safety, as it enables identification of pathogens circulating within a community before they become widespread outbreaks. Wastewater based epidemiology has blossomed in recent years due to its success in the SARS-CoV-2 pandemic, but this form of public health surveillance has a global equity problem. A large majority of the wastewater-based surveillance work has been done in large metropolitan cities with access to advanced testing infrastructure, which poses a challenge as we consider where pandemics have historically emerged. Therefore, there is a need to identify, adapt, and develop workflows to meet the needs …

Stable Oxygen And Hydrogen Isotopic Ratios Of Precipitation And Modelling Of Precipitation Sources In Central Kentucky, Usa, 2017-2022, Ariel Gold-Mccoy

Theses and Dissertations--Civil Engineering

Our goals were to carry out analyses of a multi-year δ¹⁸O and δ²H dataset of precipitation from central Kentucky and to analyze the source of precipitation for storm events from 2017 to 2022. δ¹⁸O and δ²H were measured for 244 weekly precipitation samples collected by the National Atmospheric Deposition Program at Mackville, Kentucky, USA. Modelling precipitation source was carried out using HYSPLIT model for 414 events. Results of δ¹⁸O and δ²H from central Kentucky show: consistent seasonality, including summer highs and winter lows for δ¹⁸O and δ²H; correlation with air temperature; and …