Digital Commons Network^™

Automated Hippocampal Sparing Whole Brain Radiotherapy With Simultaneous Integrated Boost For Multiple Brain Metastases: Halcyon, Hyperarc On Truebeam, And Coplanar Truebeam, Shane Mccarthy, Ryan Clark, Anthony Magliari, William St. Clair, Damodar Pokhrel

Radiation Medicine Faculty Publications

Purpose: To demonstrate the ease and feasibility that hippocampal sparing whole brain (WB) simultaneous integrated boost (HSWB-SIB) plans can be generated using knowledge-based planning and Eclipse Scripting Application Programming Interface (ESAPI) for three different modalities, HyperArc on TrueBeam (TB-HA), a coplanar beam arrangement on TrueBeam (TB-Co), and the ring-mounted Halcyon LINAC (Hal).

Methods: Twelve patients with 2–14 brain metastases were retrospectively replanned for HSWB-SIB using a published HSWB RapidPlan model with modifications for the automated addition of SIB to metastases. Prescribed dose was 30 Gy to the WB planning target volume (PTV) and 50 Gy to the metastases in 10 …

Ex Vivo Single-Molecule Analysis Of Ryanodine Receptor 2 Assembly In Cardiac And Neuronal Tissue, Qianye Yin, Christopher I. Richards

Chemistry Faculty Publications

Understanding receptor assembly is critical for elucidating the mechanisms underlying their function and regulation in physiological processes. While traditional in vitro single-molecule studies rely on isolating proteins from heterologous expression systems, they often fail to capture the in vivo physiological complexity involved in the organization and assembly of cell surface proteins. This protocol employs Total Internal Reflection Fluorescence Microscopy (TIRFM) to study GFP-tagged Ryanodine Receptor 2 (RyR2) molecules encapsulated within nanoscale vesicles. These vesicles, generated from organs rapidly extracted from the animal, effectively provide a snapshot of the receptor's assembly state at the time of extraction, enabling detailed analysis of …

Evaluating Experiential Learning In Undergraduate Agricultural Education: A Content Analysis Of Course Syllabi From A College Of Agriculture, Tyler Newberry

Theses and Dissertations--Community & Leadership Development

This study evaluated the presence and depth of experiential learning within undergraduate course syllabi from the Martin-Gatton College of Agriculture, Food, and Environment. Through a content analysis of 41 syllabi across five academic majors, the study examined how course objectives and assignments aligned with Kolb’s Experiential learning cycle (1984), Webb’s depth of knowledge (1997), and Joplin’s Mini to maxi Scale (1981). A structured coding instrument was used to quantify the frequency of learning elements across these three frameworks. The findings revealed that Abstract Conceptualization and Reflective Observation were the most common stages in both objectives and assignments, while Concrete Experience …

Biomedical Relation Extraction In The Era Of (Large) Language Models, Yuhang Jiang

Theses and Dissertations--Computer Science

Biomedical relation extraction is a key task in information extraction supporting downstream applications such as knowledge discovery, information retrieval, and question answering. As such, improvements in relation extraction (RE) are expected to have major implications for other information needs in biomedicine. In this dis- sertation, we pursue biomedical relation extraction in several advanced settings with recent advances in large language models (LLMs) and retrieval augmented generation (RAG). First, we address an important slot filling task on social media that arose during the Covid-19 pandemic, using the idea of continuous prompts with encoder models. Next, we handle a tricky dynamic n-ary …

Efficacy Of Deferoxamine Mesylate In Serum And Serum-Free Media: Adult Ventral Root Schwann Cell Survival Following Hydrogen Peroxide-Induced Cell Death, Yee Hang Ethan Ma, Abhinay R. Putta, Cyrus H. H. Chan, Stephen R. Vidman, Paula V. Monje, Giles W. Plant

Neurosurgery Faculty Publications

Schwann cell (SC) transplantation shows promise in treating spinal cord injury as a pro-regenerative agent to allow host endogenous neurons to bridge over the lesion. However, SC transplants face significant oxidative stress facilitated by ROS in the lesion, leading to poor survival. deferoxamine mesylate (DFO) is a neuroprotective agent shown to reduce H₂O₂-induced cell death in serum-containing conditions. Here we show that DFO is not necessary to induce neuroprotection under serum-free conditions by cell survival quantification and phenotypic analysis via immunohistochemistry, Hif1α and collagen IV quantification via whole cell corrected total cell fluorescence, and cell death …

German Cockroach Allergen Mitigation, Olivia Katz

Theses and Dissertations--Entomology

German cockroaches (Blatella germanica L.) remain one of the most challenging indoor pests to manage, due in large part to their cryptic nature, rapid reproduction, and resistance to insecticides. Because of their close proximity to humans, they also pose several serious health threats, including the production and dissemination of several potent allergens linked to asthma development and exacerbation. Given the risks associated with cockroach allergens, we examined the longevity of two distinct cockroach allergens (Bla g 1 and Bla g 5) in the home environment and the efficacy of cleaners at removing these allergens. Our findings show that Bla …

Including Black Farmers, Excluding Pests: Perspectives On Participation In The Cooperative Extension Service, Briana Bazile

Theses and Dissertations--Entomology

In recent years, the U.S. Department of Agriculture has faced renewed scrutiny for documented discrimination against Black farmers, yet far less attention has focused on its century-old partner in public service delivery, the Cooperative Extension Service (CES). This gap is especially notable considering 1) federal parity mandates extant since 1972 in response to significant disparities between Black and White constituents and staff,2) public commitments to Diversity, Equity & Inclusion in 2020 and, 3) the contributions of Black leaders at the origins of CES, at Tuskegee University in the late nineteenth century. Using the Kentucky Cooperative Extension Service (KYCES) as a …

Understanding Assessments For Adult English Learners: A Case Study Of Second Language Acquisition And Development, Chi Ma

Theses and Dissertations--Education Sciences

While standardized tests have become the norm for assessing language proficiency, they may not effectively capture the diversity of adult immigrants, including their backgrounds, experiences, and varying levels of language proficiency. Grounded in experiential learning theory, sociocultural theory on second language acquisition, and concepts of identity, motivation, and investment, this study explored how the Adult English Learning (AEL) program utilized different types of assessments, what the needs and goals of AEL students were in learning English, and how various stakeholders, including teachers, students, and program coordinator, perceived current assessments reflecting students’ needs and goals.

The findings of the study reveals …

Seasonal Variation Of The Stream’S Autogenic Sediment Transport Regime, Melissa Beckman

Theses and Dissertations--Civil Engineering

This thesis investigates the autogenic sediment transport regime in a low-gradient stream system in the inner bluegrass region of central Kentucky, USA The seasonality of sediment transport seasonality was not well addressed in prior research for baseflow and low flow periods, to our knowledge, due to the assumption that low flows do not account for considerable fluid transport capacity to move sediment. The hypothesis is that during the autogenic regime the sediment transport in the low-gradient system is being driven by geophysical, biogeochemical, and biological processes. We hypothesize that crayfish are one mechanical driver of sediment transport during these periods. …

Implications Of Nutrient Fate And Transport Following Nanopesticide Applications In Agricultural Field Plots In Central Kentucky, William Rud, Manuel D. Montaño, Daniel M. Miller, Wayne T. Sanderson, Carmen Agouridis, Brianna F. Benner, Tiffany L. Messer

Biosystems and Agricultural Engineering Faculty Publications

The potential benefits of nanopesticide use over standard pesticides include more precise application at reduced application rates, lower premature degradation, and decreased direct impacts to target organisms. However, field scale investigations of the fate and transport of common nanopesticides such as copper (II) hydroxide and imidacloprid combinations, remain limited. A field study evaluating nano-scale copper (II) hydroxide (Cu), standard imidacloprid (I), nanoimidicloprid (NI), and nano-scale copper (II) hydroxide and imidacloprid (CuNI) compared to control (C) plots was conducted using thirty 14.6 m² field plots to determine the impacts of nanopesticide applications on nutrient cycling and quantify the persistence of …

Folliculin (Flcn) In Thyroid Tumors: Incidence, Significance, And Role As A Driver Gene And Secondary Alteration, Faisal Hassan, Camryn Slone, Robert J. Mcdonald, Julie C. Dueber, Adeel M. Ashraf, Melina Windon, Oliver J. Fackelmayer, Cortney Y. Lee, Therese J. Bocklage, Derek B. Allison

Pathology and Laboratory Medicine Faculty Publications

Thyroid carcinomas are driven by diverse molecular alterations, but the tumor suppressor gene folliculin (FLCN), best known for its role in Birt–Hogg–Dubé (BHD) syndrome, has received limited attention in thyroid tumors. Here, we describe two thyroid tumors with pathogenic FLCN alterations—one germline and one somatic—and analyze the broader prevalence and significance of FLCN in thyroid carcinomas using multiple large sequencing datasets, including ORIEN-AVATAR. Patient 1, with a germline FLCN mutation and a history of BHD syndrome, presented with a well-circumscribed oncocytic adenoma. Molecular testing confirmed biallelic FLCN inactivation, but no additional mutations or aggressive features were observed, and the patient …

Characterizing And Targeting The Non-Catalytic Functions Of Phosphatase Of Regenerating Liver 3 (Prl-3) In Oncogenesis And Cancer Progression, Jeffery T. Jolly

Theses and Dissertations--Molecular and Cellular Biochemistry

Phosphatase of Regenerating Liver 3 (PRL-3) is frequently upregulated in various cancers and is associated with poor patient prognosis. Although traditionally studied for its phosphatase activity, PRL-3 also interacts with the CNNM family of magnesium transporters through its catalytic site, and these two functions are mutually exclusive at any given time. Most previous studies relied on a commonly used PRL-3 mutation that disrupts both phosphatase activity and CNNM binding, making it challenging to determine which function drives its oncogenic effects. To address this gap in the field, I utilized a panel of PRL-3 mutants that selectively disrupt either phosphatase activity …

Complete High Thoracic Spinal Cord Injury Causes Bowel Dysfunction In Mice, Olivia Wireman, Ellie L. Sams, Lynnet E. Richey, Gabrielle V. Hammers, Andrew N. Stewart, William M. Bailey, Samir P. Patel, John C. Gensel

Spinal Cord and Brain Injury Research Center Faculty Publications

Bowel dysfunction, is a prevalent and life-impacting comorbidity of spinal cord injury (SCI) with no long-term treatment available. SCI-induced colon changes including motility and fibrosis are understudied as are strategies to address SCI bowel dysfunction. This need remains partly due to the lack of a mouse model that recapitulates the human condition. We hypothesized that a high thoracic spinal transection in mice would trigger bowel dysfunction with coincident colon pathology similar to humans and rats after SCI. We observed bowel dysfunction as increased fecal pellet numbers within the colon, smaller pellet size, and decreased motility. Fecal pellets numbers in the …

Geographic Patterns Of Human Immunodeficiency Virus (Hiv) Risk Behaviors Among Older Adults: A Strategy For Identifying Space As A Risk Exposure, Laneshia R. Conner, Reuben Adatorwovor, Sarah Jane Robbins, Rujeko O. Machinga-Asaolu, Kathryn Showalter, Carrie B. Oser

Social Work Faculty Publications

Objectives: This study examined geographical patterns of HIV risk among adults aged 60 and older.

Methods: The 2019 Behavioral Risk Factor Surveillance System (BRFSS) weighted data was used to analyze self-reported participation in HIV risk behaviors among older adults (n = 62,852). Logistic regression models were used on a dichotomous HIV risk variable to map the distribution of HIV risk behaviors across both census regions and divisions.

Results: The weighted model revealed significance across regions. Across all regions, identifying as female, identifying as Hispanic, and reporting not having depression were protective from participating in HIV risk behaviors.

Discussion: The …

Longitudinal Relationship Between Problematic Social Networking Use And Internalizing Problems, Lindsay Roesel

Theses and Dissertations--Family Sciences

The purpose of this study is to examine the longitudinal and bidirectional relationships between problematic social networking use (PSNU) and internalizing problems (depressive symptoms and self-esteem) in adolescents. Data were collected from N = 360 adolescents from two public high schools in Turkey, 14.58 years old in 9^th grade at Time 1 (54.3% females). Participants completed four assessments regarding problematic social media use, depression and self-esteem, over the course of two school years, once per semester in 9^th and 10^th grades. The study tested a series of hypotheses which tested the bidirectional relationships between PSNU on depressive …

Serious Bacterial Infections And Hepatitis C Virus Among People Who Inject Drugs, Thomas J. Stopka, Robin M. Nance, L. Sarah Mixson, Hunter Spencer, Judith I. Tsui, Judith M. Leahy, Mai T. Pho, Jean Dejace, Judith Feinberg, April M. Young, Wei Teng Yang, Amelia Baltes, Eric Romo, Randall T. Brown, Kerry Nolte, William C. Miller, William A. Zule, Wiley D. Jenkins, Joseph A. Delaney, Peter D. Friedmann

Epidemiology and Environmental Health Faculty Publications

Limited research has examined the possible synergistic interrelationships between serious bacterial infections (SBIs) of the heart (i.e., endocarditis), bone, spine, brain, or joints (e.g., osteomylelitis) and hepatitis C virus (HCV) infections. We examined whether syndemic interactions existed between SBI, HCV, and substance-use-related factors in rural communities, hypothesizing that injection-mediated risks elevated the likelihood for both SBIs and HCV infections, which could be exacerbated by synergistic biological–biological or biological and social interactions. We calculated the prevalence ratios (PRs) of past-year SBI associated with each risk factor in separate models. Effect modification among significant risk factors was assessed using multiplicative interaction. Among …

Viral Transcript And Tumor Immune Microenvironment-Based Transcriptomic Profiling Of Hpv-Associated Head And Neck Squamous Cell Carcinoma Identifies Subtypes Associated With Prognosis, Anastasiia Nikitina, Daria Kiriy, Andrey Tyshevich, Dmitry Tychinin, Zoya Antysheva, Anastasya Sobol, Vladimir Kushnarev, Nara Shin, Jessica H. Brown, James Lewis, Krystle A. Lang Kuhs, Robert Ferris, Lori Wirth, Nikita Kotlov, Daniel L. Faden

Epidemiology and Environmental Health Faculty Publications

Human papillomavirus (HPV)-associated head and neck squamous cell carcinoma (HPV-positive HNSCC) has distinct biological characteristics from HPV-negative HNSCC. Using an AI-based analytical platform on meta cohorts, we profiled expression patterns of viral transcripts and HPV viral genome integration, and classified the tumor microenvironment (TME). Unsupervised clustering analysis revealed five distinct and novel TME subtypes across patients (immune-enriched, highly immune and B-cell enriched, fibrotic, immune-desert, and immune-enriched luminal). These TME subtypes were highly correlated with patient prognosis. In order to understand specific factors associated with prognosis, we used the unsupervised clustering of an HPV-positive HNSCC cohort from The Cancer Genome Atlas …

Fairehr, Karen S. Galea, Finlay Brooker, Shahzad Rashid, Michael Bader, Yu Ait Bamai, Jos Bessems, Embialle Mengistie Beyene, Alison Connolly, Carla Costa, Pravina Deligannu, Radu Corneliu Duca, Kaoutar Chbihi, Kasahun Eba, Manosij Ghosh, Melissa Gonzales, Stuart Harrad, Erin N. Haynes, Nancy B. Hopf, Po Chin Huang, Kate Jones, Konstantinos M. Kasiotis, Ming Kei Chung, Jihyon Kil, Holger Koch, Jung Hwan Kwon, Elizabeth Ziying Lin, Henriqueta Louro, Kyriaki Machera

Epidemiology and Environmental Health Faculty Publications

The FAIREHR (Findable, Accessible, Interoperable, Reusable Environmental and Health Registry) platform is a state-of-the-art online registry for prospective harmonization of human biomonitoring (HBM). It was developed by the HBM working group of the Europe Regional Chapter of the International Society of Exposure Science (ISES Europe) and is supported by the HBM Global Network. FAIREHR is designed to harmonize HBM metadata and support the implementation of the FAIR (Findable, Accessible, Interoperable and Reusable) Guiding Principles throughout HBM studies or programs. The registry enables preregistration of HBM by capturing key metadata on study design, metadata management, and planned methods before participant recruitment. …

Defining An Irrelevant Plasma Concentration For Trichlormethiazide In Racing Horses – A Case Report And Analysis, Kimberly Brewer, Justin C. Holmes, Clara Fenger, Abelardo Morales-Briceño, Andreas F. Lehner, George A. Maylin, Robert Holland, Levent Dirikolu, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

This communication reviews the matter of the clinical, pharmacological, and regulatory significance of a 4.95 nanogram/ml identification of trichlormethiazide in urine from a horse six days after administration of a compounded trichlormethiazide/ dexamethasone preparation. This combination of dexamethasone and trichlormethiazide has historically been widely used in bovine and equine medicine to reduce local inflammation and edema. The horse was administered an oral formulation of trichlormethiazide 400 mg /dexamethasone 10 mg per packet under veterinary supervision on days 7 and 6 before the race in question. Following the race, the post-race urine sample from the horse tested “positive” for trichlormethiazide at …

Trace Level Identifications Of N-Ethylnicotinamide In Racing Horses Associated With A Vitamin B3 Supplement: Case Reports And Analysis, Clara Fenger, Kimberly Brewer, Abelardo Morales-Briceño, Andreas F. Lehner, George A. Maylin, Robert Holland, Levent Dirikolu, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

Nikethamide, N,N-diethyl-3-pyridinecarboxamide (Coramine®) is a central nervous system stimulant at one time used therapeutically in human medicine but no longer marketed in the US. Nikethamide is classified a Drug Class 1, Penalty Class A substance by the Association of Racing Commissioners International (ARCI) and as a Horseracing Integrity and Safety Authority (HISA) SO or banned Stimulant that lacks FDA approval. Identifications of Nikethamide or its metabolite N-Ethylnicotinamide in racing samples can result in significant penalties in horse racing. Nikethamide is rapidly metabolized to N-Ethylnicotinamide, which remains detectable in plasma and urine leading to its use as a regulatory “marker” of …

Anabolic Properties Of The Progestin Altrenogest And Its Regulation As An Environmental Substance In Horse Racing – Review And Analysis, Andreas F. Lehner, Levent Dirikolu, Sarah Jacob, Kimberly Brewer, Abelardo Morales Briceñoe, Robert Holland, George Maylin, Clara Fenger, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

Altrenogest is a progestogen substance used in equine medicine for the synchronization of ovulation during the spring breeding season and the control of estrus in mares. It accomplishes its actions by down-regulation of endometrial progesterone receptors and repression of estrogen receptor transcriptional activation. However, various groups have also measured androgen receptor binding activity for altrenogest in vitro; these findings combined with androgenic and anabolic properties roughly 1/20 in potency of testosterone and its structural similarity to the anabolic steroid trenbolone have created concerns among racing authorities of altrenogest’s potential abilities to alter the outcome of equine athletic performance, even though …

The Effect Of Fructose Metabolism On Hepatic De Novo Lipogenesis And Adipose Tissue Insulin Resistance, Taghreed Ibrahim Fadhul

Theses and Dissertations--Nutritional Sciences

There is a worldwide epidemic of obesity and metabolic dysfunction. The root cause of obesity is driven by a positive energy balance, which is a consequence of increased caloric intake and decreased energy expenditure. Energy intake is the more dominant force, as increasing energy expenditure is a time-consuming and labor-intensive process. The caloric surplus can be mainly accounted for by increased consumption of high-fat and high-sugar diets. While the risks associated with fat consumption are well-documented, the impact of sugar intake is less understood.

Fructose is a simple sugar widely consumed in modern diets. Its intake has been associated with …

Spermidine And Polyamine Blockade As Key Mediators Of Intestinal Epithelial Cell Functions, Madison Lynn Flory

Theses and Dissertations--Microbiology, Immunology, and Molecular Genetics

Frequently, alterations in the composition of the gut microbiota and its metabolites are linked with intestinal diseases, including inflammatory bowel disease and colorectal cancer. However, the direct connections between alterations in the microbiome and disease states often remain elusive. Prior research has identified increases in both polyamines, which are a class of microbial metabolite, and in the abundance of bacteria that can produce polyamines, in a murine colorectal cancer model. Understanding the significance of the interaction between these microbial metabolites and intestinal epithelial cells (IECs) will provide new and expanded evidence of the importance of the gut microbiome in colorectal …

Visual Legal Rhetoric In The Age Of Generative Ai And Deepfakes: Renaissance Or Dark Ages?, Michael D. Murray

Law Faculty Scholarly Articles

The paradoxical development of visual generative AI tools, such as OpenAI’s DALL-E 3, Midjourney, and Stable Diffusion, simultaneously signals a renaissance and a potential dark age in visual rhetoric and communication. On the one hand, these tools democratize the creation of visual content, empowering attorneys and others to become artists and illustrators of their legal communications without needing to learn how to draw. These AI systems can simplify complex legal concepts, bridge language barriers, and enhance advocacy. But on the other hand, the proliferation of deepfakes presents significant challenges for visual rhetoric. Deepfakes can quickly and easily create realistic but …

Pesticide Lies! A Call For Epa Action As Recent Judicial Decisions Reveal The Harmful Truth Resulting From Exposure, Stevi Whitman

Kentucky Journal of Equine, Agriculture, & Natural Resources Law

In this note, Stevi Whitman analyzes the dangers of weak pesticide regulation in the United States. Whitman explains that the EPA’s reliance on the vague “unreasonable adverse effects” standard under the Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) allows harmful products like Roundup to remain on the market despite strong evidence of health risks. Whitman argues for strengthening U.S. pesticide laws to match the European Union’s stricter safety threshold. She proposes amending FIFRA to require proactive bans on hazardous pesticides and greater accountability from manufacturers to better protect public health and the environment.

Chronic Sleep Fragmentation Differentially Affects Alzheimer’S Disease Pathology In Male And Female AppSaa Knock-In Mice, Margaret R. Hawkins, Haleigh R. Whitlock, Carrie E. Johnson, Teresa Macheda, Ma Kayla F. Cox, Madison G. Lapid, Kelly N. Roberts, Heather M. Hash, Esther G. Drinkard, Sridhar Sunderam, Bruce F. O’Hara, Michael P. Murphy, Marilyn J. Duncan, Adam D. Bachstetter

Biology Faculty Publications

Introduction: Sleep fragmentation often precedes Alzheimer’s disease (AD) diagnosis and represents a potential modifiable risk factor, especially among women who have higher prevalence of both sleep disorders and AD. Methods: This study investigated how chronic sleep fragmentation affects neuroinflammation and amyloid-beta (Aβ) accumulation in male and female APP^SAA knock-in mice, a physiologically relevant AD model expressing APP at normal levels. APP^SAA mice of both sexes (N=8/sex/strain, 8 months old) underwent either 5 weeks of chronic sleep fragmentation administered during the light phase using an automated sweeper system or undisturbed sleep. Sleep-wake patterns and circadian rhythms were monitored using …

Simpson's Tachytely Or Bradytely? The Importance Of Quantifying Rate Uncertainty, Rosana Zenil-Ferguson, Lee Hsiang Liow

Biology Faculty Publications

The spectacular variation in species forms and richness across space and time can be explored using sophisticated and powerful tools recently developed by evolutionary modellers. In this contribution, we ask if the classic ‘Simpsonian’ view of tachytelic (fast), horotelic (standard) and bradytelic (slow) diversification rates can be distinguished with currently available tools and data. A neglected topic here is the role that the uncertainty of diversification rate estimates plays, where the lack of in-depth uncertainty measures could hinder our ability to confidently suggest differences in speciation or extinction rates in any given comparison. We propose quantifying the relative uncertainty of …

“I Just Don’T Find It Interesting”, Jennifer L. Osterhage, Utku Caybas, Jaeyun Han, Ellen L. Usher

Biology Faculty Publications

Situated expectancy-value theory (SEVT) posits that students’ expectancies for success, task values, and perceived costs affect their performance in STEM courses. Less is known about how students’ majors and timing of course completion affect motivational processes. Using the SEVT framework, this study utilised a convergent mixed methods approach to investigate academic motivation among students (N = 646) who enrolled in introductory biology at a large public university. Survey data revealed that students majoring in Biology were more motivated than students from other majors, although academic major was not directly associated with final grade. Upper-level students reported significantly lower competence-related beliefs …

Seasonal Divergence In Reproductive Timing On The Verge Of Spring, Devraj Singh, Adam M. Fudickar, Ellen D. Ketterson

Biology Faculty Publications

Every year as spring approaches and day length increases, many birds begin to reproduce, an annual expression of seasonal phenology that requires physiological preparation. In species distributed over a broad geographic range, populations that breed at higher latitudes are often migratory and delay reproduction until later in the year as compared to those breeding at lower latitudes. Dark-eyed Juncos serve as an excellent model for understanding the timing mechanisms regulating population-level variation in seasonal reproductive responses. We compared two seasonally sympatric dark-eyed junco populations in early spring. One migrates (Junco hyemalis hyemalis) and breeds in Alaska and Canada, while the …

Chromatin Remodeling Protein Bptf Mediates Chromatin Accessibility At Gene Promoters In Planarian Stem Cells, Prince Verma, John M. Allen, Alejandro Sánchez Alvarado, Elizabeth M. Duncan

Biology Faculty Publications

Background The regulation of chromatin accessibility is essential in eukaryotic cells as one of several mechanisms that ensure gene activation occurs at appropriate times and in appropriate cell types. Accordingly, mutations in chromatin remodeling proteins are linked to many different developmental disorders and cancers. One example of a chromatin protein that has been linked to both developmental abnormalities and cancer is BPTF/NURF301, the largest subunit of the Nucleosome Remodeling Factor (NuRF) complex. The BPTF subunit is not only important for the formation of NuRF but also helps direct its activity to particular regions of chromatin by preferentially binding histone H3 …