Digital Commons Network^™

Detection Of Alzheimer's Disease And Other Diseases Using An Improved Photoaffinity Labeling Method, Boyd E. Haley

Chemistry Faculty Patents

An improved method for diagnosing a disease comprising detecting a disease-specific biochemical marker macromolecule within a sample of extracted cerebral spinal fluid or serum is disclosed. In particular, a radioactively labeled photoaffinity probe is used to diagnose a disease. For instance, Alzheimer's disease can be diagnosed by detecting a disease-specific protein having a molecular weight of about 42,000 daltons, i.e., glutamine synthetase. Also included is a method for detecting other disease states, such as cancer, by detecting the presence or absence of specific nucleotide binding proteins.

Identification Of An Idiotypic Peptide Recognized By Autoantibodies In Human Immunodeficiency Virus-1-Infected Individuals, Q. L. Wang, H.-T. Wang, Edwin Blalock, Sybille Müller, Heinz Köhler

Markey Cancer Center Faculty Publications

Antibodies against HIV-1 proteins in HIV-1-infected individuals share a cross-reactive idiotype defined by the monoclonal antiidiotypic antibody 1F7 (5). Using a computer algorithm based on the molecular recognition theory, regions of inverse hydropathy between the variable sequence of 1F7 and human monoclonal anti-HIV-1 antibodies were identified, which are assumed to be involved in idiotype-antiidiotype contacts. A peptide was designed from the proposed contact in the variable heavy chain framework 3-complementarity determining region 3 (FR3-CDR3) of human antibodies and was synthesized. This peptide is recognized by the antiidiotype 1F7 and inhibits the binding of 1F7 to human anti-HIV-1 antibodies which express …

Method For Inhibiting Angiogenesis With Aurintricarboxylic Acid, Its Analogues Or Salts, Delwood C. Collins, Antonio Gagliardi, Anjan Bhattacharyya

Obstetrics and Genecology Faculty Patents

Aurintricarboxylic acid has potent anti-angiogenic activity and may be used for new therapeutic approaches for diseases of neovascularization including the treatment of solid tumors, diabetic retinopathy and arthritis, among others.

Immune Response To The Carcinoembryonic Antigen In Patients Treated With An Anti-Idiotype Antibody Vaccine, Kenneth A. Foon, Mala Chakraborty, William J. John, Amanda Sherratt, Heinz Köhler, Malaya Bhattacharya-Chatterjee

Markey Cancer Center Faculty Publications

We have generated an IgG1 murine monoclonal anti-idiotype antibody (Ab2) designated 3H1, which mimics a specific epitope on the carcinoembryonic antigen (CEA). Patients with CEA positive tumors are immunologically "tolerant" to CEA. We used 3H1 as a surrogate for CEA for vaccine therapy of 12 patients with advanced colorectal cancer. Each of the patients received a minimum of four intracutaneous injections of aluminum hydroxide precipitated 3H1 at either 1, 2, or 4 mg dosage per injection. 9 of 12 patients demonstrated anti-anti-idiotypic (Ab3) response to 3H1. All nine patients generated specific anti-CEA antibody demonstrated by reactivity with radiolabeled purified CEA; …

Non-Destructive Method For Radiolabelling Biomolecules By Halogenation, Anwar A. Hussain, Lewis W. Dittert

Pharmaceutical Sciences Faculty Patents

A kit and method of non-destructively radiolabelling a biomolecule substrate by halogenation, including the steps of: a) reacting a secondary amine with a chlorinating agent to produce an N-chloro secondary amine, (b) reacting the N-chloro secondary amine with a salt of a radiolabelled halogen to produce a radiolabelled halogenating agent, (c) reacting a solution of the biomolecule substrate with the radiolabelled halogenating agent, and (d) recovering a radiohalogenated biomolecule from the reaction of step (c). The kit and method allow for more complete iodination with a lessening of side reactions such as decomposition of the biomolecule substrate to be labelled.

College Of Law Commencement Program, 1995, University Of Kentucky

Graduation Programs

No abstract provided.

The Oral Physician... Creating A New Oral Health Professional For A New Century, David A. Nash

Oral Health Science Faculty Publications

No abstract provided.

Gender, Space And The Academy: An Interview With Doreen Massey, The Open University, Brenda Weber Ijams, Jeff Popke, Kakie Urch

disClosure: A Journal of Social Theory

No abstract provided.

Pbn, Dmpo, And Pobn Compositions And Method Of Use Thereof For Inhibition Of Age-Associated Oxidation, John M. Carney, Robert A. Floyd

Pharmacology and Nutritional Sciences Faculty Patents

Compositions containing as the active ingredient a spin-trapping reagent, preferably α-phenyl butyl nitrone (PBN) or spin-trapping derivatives thereof, in a suitable pharmaceutical carrier for administration to a patient are disclosed for treating or preventin symptoms associated with aging or other conditions associated with oxidative tissue damage. Other spin-trapping agents can also be used, such as 5,5-dimethyl pyrroline N-oxide (DMPO) or α-(4-pyridyl 1-oxide)-N-tert-butylnitrone (POBN), and other spin-trapping derivatives thereof. These compositions and methods are useful in the treatment of age-related disorders, pre-surgical and/or pre-anesthetic preparation or administration of chemotherapeutic agents, and in the treatment of disorders or trauma of the brain, …

Apparatus And Method For Noninvasive Chemical Analysis, Robert A. Lodder

Pharmaceutical Sciences Faculty Patents

A method and apparatus are provided for magnetohydrodynamic acoustic-resonance, near-IR spectroscopy. The method includes a step of applying to a subject under study a magnetic field having a strength between 2,00-10,000 gauss, near-IR radiation having a wavelength between 800-3,000 nm and an acoustic wave having a frequency between 10 khz-1 Mhz. The method also includes the steps of inducing vibration of ions in the magnetic field and detecting an electric wave generated magnetohydrodynamically by the acoustic wave induced vibration of the ions. Next is the collecting of the electrical, acoustical and near-IR spectra and the analyzing of the collected spectra. …

Current Issues In Environmental Law, Office Of Continuing Legal Education At The University Of Kentucky College Of Law, W. Henry Graddy Iv, Jack C. Bender, Thomas W. Fitzgerald, Carl W. Breeding, Marcus P. Mcgraw, Phillip J. Shepherd, David J. Smart, Dennis J. Conniff, Lloyd R. Cress Jr., E. Douglas Stephan, James L. Dickinson, Todd E. Leatherman, Elizabeth U. Natter, W. Patrick Stallard, Rick L. Thomas, Bradley E. Dillon, Vanessa M. Berge, Ronald R. Van Stockum Jr.

Continuing Legal Education Materials

Materials from the Current Issues in Environmental Law seminar held by UK/CLE in April 1995.

Tissue-Specific Expression Of Human Lipoprotein Lipase: Effect Of The 3′-Untranslated Region On Translation, Gouri Ranganathan, John M. Ong, Ada Yukht, Mehrnoosh Saghizadeh, Rosa B. Simsolo, Andrea Pauer, Philip A. Kern

Clinical and Translational Science Faculty Publications

Lipoprotein lipase (LPL) is a central enzyme in lipoprotein metabolism and is expressed predominantly in adipose tissue and muscle. In these tissues, the regulation of LPL is complex and often opposite in response to the same physiologic stimulus. In addition, much regulation of LPL occurs post-transcriptionally. The human LPL cDNA is characterized by a long 3′-untranslated region, which has two polyadenylation signals. In this report, human adipose tissue expressed two LPL mRNA species (3.2 and 3.6 kb) due to an apparent random choice of sites for mRNA polyadenylation, whereas human skeletal and heart muscle expressed predominantly the longer 3.6-kb mRNA …

[Review Of] Motif-Index Of Folk Literature: New Enl. And Rev Ed.; Cd-Rom Ed., Robert A. Aken

Library Faculty and Staff Publications

No abstract provided.

High Energy Coprecipitate Of Nonoxynol Oligomer, Pvp And Iodine Having Contraceptive And Potent Anti-Hiv Properties, George Digenis, Alexander G. Digenis

Pharmaceutical Sciences Faculty Patents

A composition which is a contraceptive with potent anti-HIV activity. The composition is a high energy coprecipitate of nonoxynol-9 oligomers, polyvinylpyrrolidone (PVP) and iodine or PVP-I and shows a pronounced synergistic, anti-HIV effect between the compounds of the composition. A method of obtaining a contraceptive and anti-HIV environment in a female comprising administering to said female an effective amount of a high energy coprecipitate.

Sustained Release Drug Delivery Devices, Thomas J. Smith, Paul Ashton, Paul A. Pearson

Ophthalmology and Visual Science Faculty Patents

A method and device for treating a mammalian organism to obtain a desired local or systemic physiological or pharmacological effect is provided. The method includes administering a sustained release drug delivery system to a mammalian organism in need of such treatment at an area wherein release of an effective agent is desired and allowing the effective agent to pass through the device in a controlled manner. The device includes an inner core or reservoir comprising the effective agent; a first coating layer, which is essentially impermeable to the passage of the effective agent; and a second coating layer, which is …

Chemical And Biological Monitoring Of A Constructed Wetland On Jones Branch Treating Acid Mine Drainage, Barbara A. Ramey, George Chalfant, Jon A. Walker

KWRRI Research Reports

Acid mine drainage (AMD) from an abandoned underground coal mine in the Jones Branch watershed in McCreary County, KY, substantially reduced water quality in Jones Branch. Downstream from the mine seeps, the pH was routinely below 4.5 and concentrations of most heavy metals, especially iron, were elevated. A cattail wetland (1,022 m²) was constructed on Jones Branch in 1988-1989 to obviate the effects of the AMD. Monthly chemical monitoring was performed on the water from above, from below, from the inlet and outlet of the wetland, and from the 25 cells within the wetland. Based on chemical monitoring, …

Sodium In Pasture Species And Grazing Livestock, C. T. Dougherty, Kenneth L. Wells, G. E. Mitchell

Agronomy Notes

Concern among some dairy nutritionists has recently been expressed that "high potassium" content of hay and silage is reducing milk production in some high-producing dairy herds. Alfalfa and grass hay and com and grass silage, which have been heavily fertilized, are the objects of this concern. The nutritional question being considered in this article is whether an animal diet excessively high in potassium (1

Risk Assessment Plan For Petroleum Underground Storage Tanks In Kentucky, Part Ll: Diesel, Heating Oil, Other Middle Distillates And Waste Oil, Wesley J. Birge, A. J. Grant, J. R. Shaw, M. D. Kercher, D. P. Keogh

KWRRI Research Reports

This report consists of an appendix :Risk Assessment Plan for Petroleum Underground Storage Tanks in Kentucky and a second appendix: Environmental Half-Life and Ecological Effects of PAHs

Atrazine And Alachlor Dissipation Rates From Field Experiments, Stephen R. Workman, Andrew D. Ward, Norman R. Fausey, Sue E. Nokes

Biosystems and Agricultural Engineering Faculty Publications

Chemical transport is being monitored in the root zone of three agricultural management systems at the Ohio Management Systems Evaluation Area (OMSEA). Atrazine and alachlor concentration data from soil cores taken to a depth of 0.9 m and partitioned into the increments of 0.0 to 0.15, 0.15 to 0.3, 0.45 to 0.6, and 0.75 to 0.9 m show the herbicides remained in the top 0.15 m of the profile during the 1991 and 1992 growing seasons. The slow movement of herbicides was partly due to below normal rainfall during the period. Since the herbicides have not been transported out of …

Risk Assessment Plan For Petroleum Underground Storage Tanks In Kentucky, Wesley J. Birge, L. C. Taylor, M. D. Kircher, A. J. Grant

KWRRI Research Reports

This study addresses the development of guidelines for corrective actions to be applied to petroleum underground storage tanks (USTs) within the Commonwealth of Kentucky. The report presents findings and recommendations for gasoline, diesel fuel, heating oil, kerosene, jet fuel, and waste oil.

Risk Assessment Plan For Petroleum Underground Storage Tanks In Kentucky, Part 1: Gasoline, Wesley J. Birge, M. D. Kercher, D. P. Keogh, L. C. Taylor

KWRRI Research Reports

This report consists of three appendix:

• I Risk Assessment Procedures and Calculations
• II BTEX Half-life Estimates in Air, Soil, Groundwater, and Surface Water
• III Environmental and Ecological Effects of BTEX

Regulatory Takings And Wetland Protection In The Post-Lucas Era, Richard C. Ausness

Law Faculty Scholarly Articles

In June 1992, the United States Supreme Court decided Lucas v. South Carolina Coastal Council. The case involved a claim for compensation against the State of South Carolina by a landowner who was prohibited from placing structures on two of his beachfront lots. The Court declared that the landowners must be compensated when government regulations deprive them of all economically beneficial or productive uses of their property unless the proscribed uses were not permitted as part of their original titles.

Although some legal commentators have praised the Lucas decision, others have strongly condemned it. A common criticism of Lucas …

A Survey Of Source And Finished Water Supplies For Giardia Cysts And Cryptosporidium Oocysts, Lisa Martin Sexton

KWRRI Research Reports

The occurrence of Giardia lamblia cysts and Cryptosporidium parvum oocysts was determined from source and treated drinking water supplies at selected water treatment facilities in Eastern Kentucky. Giardia and Cryptosporidium are two human protozoan parasites often found in water supplies due to fecal contamination. Characteristic cysts and oocysts were identified from large volume water samples, typically 100 gallons for raw samples and 1,000 gallons for finished water, using an indirect fluorescent antibody specific for Giardia and Cryptosporidium. Source water from four different water treatment facilities utilizing a reservoir, river, and two creeks were assayed. All sites exhibited Giardia lamblia …

The Biological Speciation And Toxicokinetics Of Aluminum, Emily Devoto, Robert A. Yokel

Pharmaceutical Sciences Faculty Publications

This review discusses recent literature on the chemical and physiological factors that influence the absorption, distribution, and excretion of aluminum in mammals, with particular regard to gastrointestinal absorption and speciation in plasma. Humans encounter aluminum, a ubiquitous yet highly insoluble element in most forms, in foods, drinking water, and pharmaceuticals. Exposure also occurs by inhalation of dust and aerosols, particularly in occupational settings. Absorption from the gut depends largely on pH and the presence of complexing ligands, particularly carboxylic acids, with which the metal can form absorbable neutral aluminum species. Uremic animals and humans experience higher than normal body burdens …

The Life-Long Learning Imperative... Ends And Means, David A. Nash

Oral Health Science Faculty Publications

No abstract provided.

Use Of Riparian Vegetated Filter Strips To Reduce Nitrate And Fecal Contamination In Surface Water, Mark S. Coyne, Robert L. Blevins, Rebecca A. Gilfillen

KWRRI Research Reports

This research assessed fecal bacteria trapping in surface runoff by grass filters and their potential to enhance NO₃^- removal via denitrification. Grass filter strips 9.0 m long trapped over 99% of the soil in surface runoff in 1992. Fecal coliform removal was less than 75%. In 1993, 9.0 and 4.5 m grass filter strips trapped 99 and 95% of the sediment, respectively. Fecal coliform trapping efficiency was 90% in 9.0 m grass filters and 75% in 4.5 m filters. Fecal streptococci trapping efficiency was 77% in 9.0 m grass filters and only 56% in 4.5 m filters. Fecal …

Review: If You Seduce A Straight Person, Can You Make Them Gay?: Issues In Biological Essentialism Versus Social Constructionism In Gay And Lesbian Identities (John P. Dececco And John P. Elia, Harrington Park, 1993), James M. Donovan

Law Faculty Scholarly Articles

No abstract provided.

Finding Your Own Lake Wobegon, Michael R. Nichols

Kentucky's Annual Forum on Transportation

No abstract provided.

Cystic Fibrosis Transmembrane Conductance Regulator Mutations That Disrupt Nucleotide Binding, James Logan, David Hiestand, Paru Daram, Zhen Huang, Donald D. Muccio, John Hartman, Boyd Haley, William J. Cook, Eric J. Sorscher

Molecular and Cellular Biochemistry Faculty Publications

Increasing evidence suggests heterogeneity in the molecular pathogenesis of cystic fibrosis (CF). Mutations such as deletion of phenylalanine at position 508 (delta F508) within the cystic fibrosis transmembrane conductance regulator (CFTR), for example, appear to cause disease by abrogating normal biosynthetic processing, a mechanism which results in retention and degradation of the mutant protein within the endoplasmic reticulum. Other mutations, such as the relatively common glycine-->aspartic acid replacement at CFTR position 551 (G551D) appear to be normally processed, and therefore must cause disease through some other mechanism. Because delta F508 and G551D both occur within a predicted nucleotide binding …

Nitrous Oxide Flux From Poultry-Manured Erosion Plots And Grass Filters After Simulated Rain, Mark S. Coyne, R. A. Gilfillen, Robert L. Blevins

Plant and Soil Sciences Faculty Publications

Adding carbon-rich materials to fields, like manure, may enhance denitrification. Grass filters, which are used to trap surface runoff from these fields, may also provide a carbon-rich environment that favors water infiltration and denitrification. Nitrous oxide (N₂O) may be evolved these settings. It is a radiatively important trace gas and intermediate in the denitrification pathway and several other microbial processes. We measured N₂O flux, after simulated rain, using a soil cover technique in poultry-manured plots and grass filters receiving their runoff. Intact soil cores were used to relate the N₂O flux to the denitrification …