Digital Commons Network^™

Nucleotide Or Nucleoside Photoaffinity Compound Modified Antibodies, Methods For Their Manufacture And Use Thereof As Diagnostics And Therapeutics, Boyd E. Haley, Heinz Köhler, Krishnan Rajagopalan, Gabriela Pavlinkova

Chemistry Faculty Patents

Sites on antibodies having affinity for photoaffinity compounds, in particular purine or azidopurine containing compounds are taught. These sites provide for the site-specific attachment of nucleotide photoaffinity compounds to antibodies, e.g., ATP- or GTP-analog photoaffinity compounds by photochemical reaction. These nucleotide photoaffinity compounds may additionally be attached to molecules having a desired therapeutic or diagnostic activity, and the resultant conjugates used as diagnostics or therapeutics.

The Criminal Law Implications Of The Human Genome Project: Reimagining A Genetically Oriented Criminal Justice System, Steven I. Friedland

Kentucky Law Journal

No abstract provided.

Indicator Bacteria Concentrations Of Two Northwest Arkansas Streams In Relation To Flow And Season, Dwayne R. Edwards, Mark S. Coyne, Tommy C. Daniel, P. F. Vendrell, J. F. Murdoch, P. A. Moore Jr.

Biosystems and Agricultural Engineering Faculty Publications

Concentrations of indicator bacteria such as fecal coliform (FC) and fecal streptococcus (FS) are often used to assess the suitability of waters for their intended use(s) and to allocate resources for water quality improvement measures. There is evidence, however, that concentrations of FC and FS can be influenced by variables such as season and flow rate during sampling, which could lead to biased results. The objective of this study was to assess the impacts of season and flow rate on concentrations of FC and FS. Fecal indicator bacteria concentrations were measured for approximately three years at five sites on two …

Translational Regulation Of Lipoprotein Lipase By Epinephrine Involves A Trans-Acting Binding Protein Interacting With The 3′ Untranslated Region, Gouri Ranganathan, Diane Vu, Philip A. Kern

Clinical and Translational Science Faculty Publications

To better characterize the translational regulation of lipoprotein lipase (LPL) by epinephrine, cytoplasmic extracts were prepared from 3T3-L1 adipocytes, 3T3-F442A adipocytes, and other nonadipocyte cell lines (C2 cells, 3T3 fibroblasts, and Chinese hamster ovary cells). After treatment with epinephrine, cell extracts from the adipocytes inhibited LPL translation in an in vitro translation assay, whereas extracts from the C2 cells and 3T3 fibroblasts did not affect LPL translation. To identify the region on the LPL mRNA that controlled translation, in vitro translation was carried out using constructs containing different LPL sequences. Specific deletion of the first 50 (1601-1650) nucleotides of the …

Can Denitrification Reduce No3 In Shallow Ground Water?, Mark S. Coyne, E. Montgomery, Grant W. Thomas

Agronomy Notes

Systematic sampling of springs, tiles, and wells in Kentucky, as part of a recent statewide program to assess agricultural impacts on water quality, showed that NO₃ concentrations in these shallow ground water sources varied tremendously. The NO₃ concentration could be correlated with flow rate; higher when ground water recharge flushed NO₃ from soil in winter and spring, and lower or non detectable in summer and fall when less NO₃ leaching occurred. Depending on the season, NO₃ concentrations ranged from < 1 to > 10 ppm NO₃-N in almost half of the sites. For example, the water in …

Soil Management For Intensive Grazing, Kenneth L. Wells, Charles T. Dougherty

Soil Science News and Views

Recycling of plant nutrients is of major concern in managing paddocks in pasturefields for intensive grazing. Redistribution of nutrients present in fecal and urine deposits is an important issue in growing climatically and seasonally-adapted forage species and for efficient conversion of herbage into animal products while adding to the sustainability of the system. Some of the questions that arise in managing soils for intensive grazing are discussed below.

Genetics, Genetic Testing, And The Specter Of Discrimination: A Discussion Using Hypothetical Cases, Richard H. Underwood, Ronald C. Cadle

Law Faculty Scholarly Articles

A "genetic revolution" is upon us. Techniques for genetic testing have increased in sophistication, and an international effort to map and sequence human DNA—The Human Genome Project ("HGP")—is now well under way. We are beginning to exploit our new found genetic knowledge. Recognition of the relationship between developments in genetic science, law, and public policy, is creeping into the "literature" and into the law school curriculum. Even the popular 60 Minutes television "news magazine" recently did a program on the perils of genetic testing. Still, for lawyers and policymakers at least, the material is not all that accessible.

The following …

Fresh-Water Aquifer In The Knox Group (Cambrian–Ordovician) Of Central Kentucky, James A. Kipp

Report of Investigations--KGS

Fresh water can be found in Cambrian and Ordovician carbonate rocks of the Knox Group in central Kentucky. The top of the aquifer is as much as 300 ft above mean sea level (m.s.l.) on the crest of the Cincinnati Arch, but descends off the flanks of the arch. Water is normally found in the upper 100 to 250 ft of the Knox, primarily in secondary porosity apparently associated with the unconformity at the top of the unit. Knox wells commonly exceed 750 ft in total depth, but because the aquifer is artesian, water rises to an elevation of about …

An Alternative To Ready, Fire, Aim: A New Framework To Link Environmental Targets In Environmental Law, Michael P. Vandenbergh

Kentucky Law Journal

No abstract provided.

Calibration Of A Water-Quality Model For Herrington Lake Using Empirically Derived Measurements Of Phytoplankton Growth And Nutrient Assimilation, Paul A. Bukaveckas, Angela S. Crain

KWRRI Research Reports

Importance of light limitation, nutrient availability, and hydrology in controlling the abundance and composition of the phytoplankton community of Herrington Lake (KY) was investigated over a two-year period. Selected environmental parameters were measured every two weeks (April-October) at five sampling stations located along the longitudinal gradient of the reservoir. In addition, short-term (48hr) nutrient enrichment experiments were conducted to assess the spatial and temporal variations in nutrient limitation. Phytoplankton growth responses to the combined addition of nitrogen (NO₃) and phosphorus (PO₄) were greater than those resulting from the addition of either nutrient alone. These results indicate …

Role Of Egr-1 Gene Expression In B Cell Receptor-Induced Apoptosis In An Immature B Cell Lymphoma, Subramanian Muthukkumar, Seong-Su Han, Sumathi Muthukkumar, Vivek M. Rangnekar, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Ligation of B cell receptor (BCR) on BKS-2, an immature B cell lymphoma by anti-IgM antibodies (Ab) caused apoptosis. Here we report that signaling through B cell receptor in wild type BKS-2 cells down-regulated the expression of Egr-1, a zinc finger-containing transcription factor. A reduction in the level of Egr-1 mRNA could be demonstrated as early as 30 min after the ligation of BCR on BKS-2 cells. Immunocytochemical and Western blot analysis revealed that the expression of EGR-1 protein was also inhibited by anti-IgM treatment. Antisense oligonucleotides to Egr-1 caused growth inhibition and apoptosis in BKS-2 cells, suggesting that expression …

Library Instruction Vs. Information Competency: It's Not Just Bibliographic Instruction Anymore, Antoinette Paris Powell, Patricia S. Wilson

Library Faculty and Staff Publications

The Agriculture Library at the University of Kentucky has offered library instruction in some form for the past 23 years. The program began in 1974 with the Library Literacy program that offered on demand workshops, an undergraduate for-credit course, graduate workshops and programs to extension personnel. By 1988 the program had moved into a library literacy program that was proactive, provided individualized presentations, and provided a mix of electronic and paper sources. This program gave way to the information literacy program of 1994 which expanded target groups and collaborated with other areas. A shift in the teaching emphasis in the …

Vegetative Filter Strip Removal Of Metals In Runoff From Poultry Litter-Amended Fescuegrass Plots, Dwayne R. Edwards, P. A. Moore Jr., Tommy C. Daniel, Puneet Srivastava, D. J. Nichols

Biosystems and Agricultural Engineering Faculty Publications

Runoff from land areas amended with poultry (Gallus gallus domesticus) manure can contain elevated concentrations of metals such as Cu, Fe, and Zn. Vegetative filter strips (VFS) can reduce runoff concentrations of animal manure components, but reported studies have typically focused on nutrients and solids rather than metals. This experiment assessed the impact of VFS length (0 to 12 m) on concentrations and mass losses of Cu, Fe, K, Na, Ni, and Zn in runoff from fescuegrass (Festuca arundinacea Schreb.) plots (1.5 m wide × 6 and 12 m long) treated with poultry litter. The runoff was produced from simulated …

Solving The Insurance/Genetic Fair/Unfair Discrimination Dilemma In Light Of The Human Genome Project, Eric Mills Holmes

Kentucky Law Journal

No abstract provided.

Genetics, Genetic Testing, And The Specter Of Discrimination: A Discussion Using Hypothetical Cases, Richard H. Underwood, Ronald G. Cadle

Kentucky Law Journal

No abstract provided.

The Use Of Forensic Dna In Criminal Cases In Kentucky As Compared With Selected Other States, Judith E. Lewter

Kentucky Law Journal

No abstract provided.

Goodwin V. Turner: A Comparison Of American And Jewish Legal Perspectives On Procreation Rights Of Prisoners, Daniel Pollack, Chaim Steinmetz, Andrea Tellerman

Kentucky Law Journal

No abstract provided.

Still Dirty After Twenty-Five Years: Water Quality Standard Enforcement And The Availability Of Citizen Suits, Michael P. Healy

Law Faculty Scholarly Articles

When Congress enacted the Federal Water Pollution Control Act, commonly known as the Clean Water Act, it established a pollution control regime that imposed a baseline level of technology-based pollution control, and was designed to ensure that water quality would not fall below certain standards. Twenty-five years after the enactment of the Clean Water Act, success may be claimed with regard to technology-based controls. Achieving water quality standard (WQS) compliance has proved much more difficult. Indeed, evaluated from a variety of perspectives, the enforcement of the water quality-based system of pollution control must be viewed as a failure.

In light …

Method For Obtaining Antifungal And Herbicidal Compounds That Target The First Committed Step In Shingolipid Long-Chain Base Biosynthesis, Robert C. Dickson, Robert L. Lester

Molecular and Cellular Biochemistry Faculty Patents

The invention provides the LCB1 and LCB2 genes of the yeast Saccharomyces cerevisiae that encode subunits of the enzyme serine palmitoyltransferase (SPT), the first enzyme leading to synthesis of the long-base component of the sphingolipids. The present specification describes the isolation of the LCB1 and LCB2 genes. The invention further relates to methods of using these genes to either inhibit SPT activity or to inhibit synthesis of the enzyme. Furthermore, the invention relates to methods for constructing strains of S. cerevisiae or other organisms that can be used to select and to test for compounds that either inhibit SPT activity …

Method And Compositions For Treating Age Related Disorders, John M. Carney, Robert A. Floyd

Pharmacology and Nutritional Sciences Faculty Patents

Compositions containing as the active ingredient a spin-trapping reagent, preferably α-phenyl butyl nitrone (PBN) or spin-trapping derivatives thereof, in a suitable pharmaceutical carrier for administration to a patient are disclosed for treating or preventing symptoms associated with aging or other conditions associated with oxidative tissue damage. Other spin-trapping agents can also be used, such as 5,5-dimethyl pyrroline N-oxide (DMPO) or α-(4-pyridyl 1-oxide)-N-tert-butylnitrone (POBN), and other spin-trapping derivatives thereof. These compositions and methods are useful in the treatment of age-related disorders, pre-surgical and/or pre-anesthetic preparation or administration of chemotherapeutic agents, and in the treatment of disorders or trauma of the brain, …

Cellular Basis Of Decreased Immune Responses To Pneumococcal Vaccines In Aged Mice, Manju Garg, Wei Luo, Alan M. Kaplan, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Previously, model systems were developed in our laboratory to study murine immune responses to the 23-valent pneumococcal polysaccharide vaccine Pnu-Imune, both in vivo and in vitro (M. Garg and B. Subbarao, Infect. Immun. 60:2329-2336, 1992; M. Garg, A. M. Kaplan, and S. Bondada, J. Immunol. 152: 1589-1596, 1994). Using these systems, we found that aged mice did not respond to the vaccine in vivo or in vitro. Cell separation studies showed that the unresponsiveness of the aged spleen cells to the vaccine was not due to an intrinsic B-cell defect or to T-cell-mediated immunosuppression but resulted from an accessory cell …

Apparatus And Method For Noninvasive Chemical Analysis, Robert A. Lodder

Pharmaceutical Sciences Faculty Patents

A method and apparatus are provided for magnetohydrodynamic acoustic-resonance, near-IR spectroscopy. The method includes a step of applying to a subject under study a magnetic field having a strength between 2,00-10,000 gauss, near-IR radiation having a wavelength between 800-3,000 nm and an acoustic wave having a frequency between 10 khz -1 Mhz. The method also includes the steps of inducing vibration of ions in the magnetic field and detecting an electric wave generated magnetohydrodynamically by the acoustic wave induced vibration of the ions. Next is the collecting of the electrical, acoustical and near-IR spectra and the analyzing of the collected …

Use Of L-Canavanine As A Chemotherapeutic Agent For The Treatment Of Pancreatic Cancer, Peter Crooks, Gerald A. Rosenthal

Pharmaceutical Sciences Faculty Patents

A pharmaceutical composition of canavanine, and a method treatment of cancer, particularly pancreatic cancer with L-canavanine is disclosed.

The Protective Role Of Manganese Superoxide Dismutase Against Adriamycin-Induced Acute Cardiac Toxicity In Transgenic Mice, Hsiu-Chuan Yen, Terry D. Oberley, Satit Vichitbandha, Ye-Shih Ho, Daret K. St. Clair

Toxicology and Cancer Biology Faculty Publications

Adriamycin (ADR) is a potent anticancer drug known to cause severe cardiac toxicity. Although ADR generates free radicals, the role of free radicals in the development of cardiac toxicity and the intracellular target for ADR-induced cardiac toxicity are still not well understood. We produced three transgenic mice lines expressing increased levels of human manganese superoxide dismutase (MnSOD), a mitochondrial enzyme, as an animal model to investigate the role of ADR-mediated free radical generation in mitochondria. The human MnSOD was expressed, functionally active, and properly transported into mitochondria in the heart of transgenic mice. The levels of copper-zinc SOD, catalase, and …

Apparatus And Method For Restoring Eyelid Function, Robert S. Baker, Brian J. Willoughby, Robert R. Marshall

Ophthalmology and Visual Science Faculty Patents

An apparatus is provided to restore eyelid function in a patient unable to voluntarily raise an eyelid. The apparatus includes a spiral torsion spring and pulley arrangement mounted in a housing that is implanted in the superior portion of the orbit of the eye. A wire connects the pulley to the eyelid. A spiral torsion spring provides the necessary spring force in tension to overcome the weight of the eyelid and draw the eyelid open. The natural muscles of eye closure are, however, sufficiently strong to overcome the spring tension thereby paying out wire from the pulley and closing the …

Rust Diseases Of Apple, John R. Hartman

Agriculture and Natural Resources Publications

Three related rust diseases occur on apple trees in Kentucky: cedar-apple rust, cedar-hawthorn rust, and cedarquince rust. Crabapple, hawthorn, mountain ash, pear, and serviceberry are also susceptible to these diseases. All three rusts are caused by different species of the fungus Gymnosporangium, each of which must spend a phase of its life cycle as a parasite on Juniperus species such as native red cedars or ornamental junipers.

Although cedar rusts can cause unsightly growths on Juniperus, they do not usually cause serious damage to these plants. Rust diseases can cause serious losses on apples, however, as a result …

Maintaining Conventional Septic Systems, William O. Thom, Pat Keefe

Agriculture and Natural Resources Publications

Septic systems are used in Kentucky where municipal sewage treatment is not available. They are an effective, longstanding method of collecting, treating, and disposing of homeowner wastewater, provided they are properly sited, installed, and maintained. However, a well-sited, properly sized and installed system will fail if not properly maintained. A failed septic system creates problems such as noxious odors, lowered property values, surface water contamination, and groundwater pollution and may be a health hazard. Repair and replacement costs are considerable. Additional information on assessing septic system performance and reducing the potential risk of groundwater contamination is available from a KY-A-Syst …

Twig, Branch, And Stem Diseases Of Pine, John R. Hartman

Agriculture and Natural Resources Publications

Tip Blight

Tip blight, caused by the fungus Sphaeropsis sapinea (formerly Diplodia pinea), is a serious disease of mature Austrian, Scots (Scotch), and Mugo pines in Kentucky. In the landscape, tip blight is normally not observed until pines reach about 12 years old and begin to bear cones. Severe infections year after year can greatly weaken and eventually kill affected pines.

Formulation Enhanced Transport Of A Soil Applied Herbicide, V. P. Evangelou, Louis M. Mcdonald, Sandra Prima

KWRRI Research Reports

Because pesticides are applied as formulated particles and the affinity of the active ingredient for the formulation is higher than for the bulk water, we hypothesized that a formulation complex could affect active ingredient transport. Our objectives were to investigate the nature and extent of surfactant-atrazine-clay/oxide surface interactions. When atrazine and an anionic surfactant were dried onto plain or Fe-coated sand and leached, atrazine concentrations in the initial leachate were lower in the Fe-coated sand treatment. This was likely due to an electrostatic attraction between the sand and surfactant. When a nonionic surfactant was used, atrazine concentration in the initial …

Metal-Resistance Genetically Engineered Bacteria, Sylvia Daunert, Donna Scott, Sridhar Ramanathan

KWRRI Research Reports

Bacterial-based electrochemical and optical sensing systems that respond in a highly selective and sensitive manner to antimonite and arsenite have been developed. This was accomplished by using genetically engineered bacteria bearing one of two plasmids constructed for our studies. The first plasmid, pBGD23, contains the operator/promoter region (O/P) and the gene of the ArsR protein from the ars operon upstream from the β-galactosidase gene. In the absence of antimonite/arsenite, ArsR binds to the 0/P site and prevents the transcription of the genes for ArsR and β-galactosidase, thus blocking expression of these proteins. When antimonite or arsenite is present in the …