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Modeling And Quantitative Analysis Of White Matter Fiber Tracts In Diffusion Tensor Imaging, Xuwei Liang

University of Kentucky Doctoral Dissertations

Diffusion tensor imaging (DTI) is a structural magnetic resonance imaging (MRI) technique to record incoherent motion of water molecules and has been used to detect micro structural white matter alterations in clinical studies to explore certain brain disorders. A variety of DTI based techniques for detecting brain disorders and facilitating clinical group analysis have been developed in the past few years. However, there are two crucial issues that have great impacts on the performance of those algorithms. One is that brain neural pathways appear in complicated 3D structures which are inappropriate and inaccurate to be approximated by simple 2D structures, …

Interactions And Effects Of Biomolecules On Au Nanomaterial Surfaces, Manish Sethi

University of Kentucky Doctoral Dissertations

Au nanoparticles are increasingly being used in biological applications. Their use is of interest based upon their unique properties that are achieved at the nanoscale, which includes strong optical absorbances that are size and aggregation state dependent. Such absorbances can be used in sensitive chemical/biological detection schemes where bioligands can be directly attached to the nanoparticle surface using facile methods. Unfortunately, a number of complications persist that prevent their wide-scale use. These limitations include minimal nanoparticle stability in biological-based media of high ionic strength, unknown surface functionalization effects using simple biomolecules, and determining the binding motifs of the ligands to …

Three Essays Evaluating Choices Of Teachers And Administrators In Kentucky Public Schools, Nathan Barrett

University of Kentucky Doctoral Dissertations

Public K-12 education is a large enterprise in the United States. Through local, state and federal sources, the U.S. allocated over $610 billion to K-12 public education in 2009 (NCES). Not only is the commitment of public funds for education substantial, the provision of K-12 education is primarily administered by the government in non-market settings through local school districts. It is this institutional environment that generates the impetus for evaluating how those in education make choices in the absence of markets.

Like traditional markets, non-market solutions often fail because the incentives facing individuals and agencies elicit choices which produce outcomes …

The Influence Of Antidiabetic Medications On The Development And Progression Of Prostate Cancer, Anna Elizabeth Hitron

University of Kentucky Master's Theses

The development of prostate tumors has been linked to co-morbid diabetes mellitus (DM) in several studies, potentially through the stimulation of insulin-like growth factor receptor (IGFR). This study evaluates the effect of anti-diabetic medication use on the development of high grade tumors and time to tumor progression compared to non-diabetics. This retrospective, nested case control study identified patients with prostate cancer (PCa) from the Kentucky Medicaid Database. Cases were diagnosed with PCa and DM and using at least one of the following antidiabetic medications; sulfonylureas, insulin, metformin or TZDs. Cases were further stratified on their insulin exposure resulting from therapy. …

Particulate Organic Carbon Fate And Transport In A Lowland, Temperate Watershed, William Isaac Ford Iii

University of Kentucky Master's Theses

Small lowland agricultural systems promote conditions where benthic biological communities can thrive. These biogeochemical processes have significant impacts on terrestrial ecosystem processes including POC flux and fate, nutrient balances, water quality budges, and aquatic biological functioning. Limited information is available on coupled biological and hydrologic processes in fluvial systems. This study investigates the mixture of biological and hydrologic processes in the benthic layer in order to understand POC cycling in the South Elkhorn system. Further, comprehensive modeling of POC flux in lowland systems has not been performed previously and the behavior of potentially controlling variables, such as hydrologic forcing and …

Disjuncture Among Classic Period Cultural Landscapes In The Tuxtla Mountains, Southern Veracruz, Mexico, Wesley Durrell Stoner

University of Kentucky Doctoral Dissertations

Teotihuacan was the most influential city in the Classic Mesoamerican worldsystem. Like other influential cities in the ancient world, however, Teotihuacan did not homogenously affect the various cultural landscapes that thrived in Mesoamerica during the Classic period (300-900 CE). Even where strong central Mexican influences appear outside the Basin of Mexico, the nature, extent, and strength of these influences are discontinuous over time and space. Every place within the Classic Mesoamerican landscape has a unique Teotihuacan story. In the Tuxtla Mountains of southern Veracruz, Mexico, Matacapan, located in the Catemaco Valley, drew heavily upon ideas and symbols fostered at Teotihuacan, …

Small Mammal Populations In Switchgrass Stands Managed For Biomass Production Compared To Hay And Corn Fields In Kentucky, Laura Mary Jane Schwer

University of Kentucky Master's Theses

Switchgrass (Panicum virgatum), a native warm-season grass, has been investigated as a renewable energy crop that may provide viable wildlife habitat. This study investigated small mammal populations in switchgrass, hay, and corn to assess the relative habitat quality. Four, three-night trapping sessions were conducted at four locations in Kentucky using Sherman livetraps. Trapping occurred in spring (before first hay harvest), summer, fall (before switchgrass and corn harvest), and winter (post-harvest). Relative abundance of small mammals, calculated using a capture per unit effort index (per 100 trapnights), and mean taxonomic richness were used to compare habitats. Switchgrass had a …

Experimental Flow Visualization For Corrugated Airfoils At Low Reynolds Number Including Development Of A Pitch And Plunge Fixture, Jeremy Ryan Sparks

University of Kentucky Master's Theses

Micro Air Vehicles (MAV’s) have small size and extreme maneuverability which makes them ideal for surveillance. Propulsion mechanisms include propellers, rotors, and flapping airfoils. Flapping motions, along with biologically-inspired wing profiles, are of interest due to their use of natural physics. Corrugated airfoil structures appears to have poor aerodynamic performance at higher Reynolds numbers, but serve well at Re<10,000. Understanding flow structures around corrugated profiles and comparing them to a standard airfoil will aid in understanding how these corrugated profiles perform well and have been adopted by some of nature’s most acrobatic flyers. Motivation for this investigation is to compare static flow visualizations of corrugated profiles to a standard National Advisory Committee for Aeronautics (NACA) airfoil from low to high angles of attack and further observe flow structure development of a pitching and plunging flat plate at a Re<10,000 and a Strouhal number relevant to natural fliers. The static visualization was conducted at Re=1,000 with a NACA 0012 airfoil and two corrugated models. The Pitch and Plunge Fixture (PPF) developed was constructed by simplifying flapping wings as a two degree of freedom motion in plunge (translation) and pitch (rotation). Results obtained from the PPF were compared with a numerical simulation.

Abundant Degenerate Miniature Inverted-Repeat Transposable Elements In Genomes Of Epichloid Fungal Endophytes Of Grasses, Damien J Fleetwood, Anar K Khan, Richard D Johnson, Carolyn A Young, Shipra Mittal, Ruth E Wrenn, Uljana Hesse, Simon J Foster, Christopher L. Schardl, Barry Scott

Plant Pathology Faculty Publications

Miniature inverted-repeat transposable elements (MITEs) are abundant repeat elements in plant and animal genomes; however, there are few analyses of these elements in fungal genomes. Analysis of the draft genome sequence of the fungal endophyte Epichloë festucae revealed 13 MITE families that make up almost 1% of the E. festucae genome, and relics of putative autonomous parent elements were identified for three families. Sequence and DNA hybridization analyses suggest that at least some of the MITEs identified in the study were active early in the evolution of Epichloë but are not found in closely related genera. Analysis of MITE integration …

A Computational Tool For Estimating Off-Target Application Areas In Agricultural Fields, Rodrigo S. Zandonadi, Joe D. Luck, Timothy S. Stombaugh, Michael P. Sama, Scott A. Shearer

Biosystems and Agricultural Engineering Faculty Publications

A computational method for estimating off-target application areas based on the machine-controlled section width and the field shape was developed and implemented in software with a graphical user interface written in the MatLab environment. The program, which is called the Field Coverage Analysis Tool (FieldCAT), includes three modules: data import, data preparation, and coverage analysis. Nine field boundaries were evaluated to test the software using controlled section widths from 0.5 to 27 m and various swath orientations. The estimated off-target application area from the widest section width varied from 9% to 24% depending on the shape and size of the …

Lipopolysaccharide Animal Models For Parkinson's Disease, Mei Liu, Guoying Bing

Neuroscience Faculty Publications

Lipopolysaccharide (LPS), an endotoxin from Gram-negative bacteria, acts as a potent stimulator of microglia and has been used to study the inflammatory process in the pathogenesis of Parkinson's disease (PD) and anti-inflammatory therapy for PD treatment. Here, we review the growing body of literature on both in vitro and in vivo LPS PD models. Primary cell cultures from mesencephalic tissue were exposed to LPS in vitro; LPS was stereotaxically injected into the substantia nigra, striatum, or globus pallidus of brain or injected into the peritoneal cavity of the animal in vivo. In conclusion, the LPS PD models are summarized as …

Mitochondria, Amyloid Β, And Alzheimer's Disease, Ryan Douglas Readnower, Andrew David Sauerbeck, Patrick G. Sullivan

Spinal Cord and Brain Injury Research Center Faculty Publications

Hypometabolism is a hallmark of Alzheimer's disease (AD) and implicates a mitochondrial role in the neuropathology associated with AD. Mitochondrial amyloid-beta (Aβ) accumulation precedes extracellular Aβ deposition. In addition to increasing oxidative stress, Aβ has been shown to directly inhibit mitochondrial enzymes. Inhibition of mitochondrial enzymes as a result of oxidative damage or Aβ interaction perpetuates oxidative stress and leads to a hypometabolic state. Additionally, Aβ has also been shown to interact with cyclophilin D, a component of the mitochondrial permeability transition pore, which may promote cell death. Therefore, ample evidence exists indicating that …

The Role Of Pragmatic Language Use In Mediating The Relation Between Hyperactivity And Inattention And Social Skills Problems, Melinda A. Leonard, Richard Milich, Elizabeth P. Lorch

Psychology Faculty Publications

Purpose: The current study explored whether pragmatic language use was associated with, and perhaps accounted for, the social skills problems children with varying levels of hyperactivity and inattention experience.

Method: A community sample of 54 children aged 9 to 11 years participated. Pragmatic language use, hyperactivity and inattention, and social skills were examined utilizing data collected from standardized parent-report rating scales.

Results: Pragmatic language use fully mediated the relation between hyperactivity and social skills problems, and partially mediated the relation between inattention and social skills problems. Further, pragmatic language use provided a unique contribution in the estimate …

Gastronomy And Otherness In Alphonso X’S Works: Food Identities In Cartography, Dianne Burke Moneypenny

University of Kentucky Doctoral Dissertations

This thesis investigates Alphonso X's General Estoria, Estoria de Espanna, Cantigas de Santa María, Siete Partidas, and other writings in the alphonsine corpus to illustrate the concept of classification through diet. The work of this thesis is to reveal the gastronomic connections between central and peripheral relations in cartography. Through food symbolism and dietary behaviors, cuisine functions as the purveyor of an unrivalled sketch of a text’s characters and the social conditions of the text’s production. Once unraveled, these highly socialized norms of consumption confirm that diet and identity are inextricably linked and lead to a greater understanding of medieval …

Genotoxin-Induced Acetylation Of The Werner Syndrome Protein (Wrn) And Effect On Its Dna Metabolic Function, Enerlyn Meliza Lozada Santiago

University of Kentucky Doctoral Dissertations

Loss of function of the WRN protein causes the genetic disorder Werner Syndrome that is characterized by increased cancer and premature aging. WRN belongs to the RecQ helicase family that plays key roles in preventing genome instability. In response to treatment with genotoxins, WRN is subject to post-translational modification. The relationship of post-translational modification of WRN with its function in DNA metabolism is unknown. There is accumulating evidence suggesting that WRN contributes to the maintenance of genomic integrity through its involvement in DNA replication. Consistent with this notion, WS cells are sensitive to DNA replication inhibitors and DNA damaging agents …

Three Dimensional Modeling And Animation Of Facial Expressions, Alice J. Lin

University of Kentucky Doctoral Dissertations

Facial expression and animation are important aspects of the 3D environment featuring human characters. These animations are frequently used in many kinds of applications and there have been many efforts to increase the realism. Three aspects are still stimulating active research: the detailed subtle facial expressions, the process of rigging a face, and the transfer of an expression from one person to another. This dissertation focuses on the above three aspects.

A system for freely designing and creating detailed, dynamic, and animated facial expressions is developed. The presented pattern functions produce detailed and animated facial expressions. The system produces realistic …

Molecular And Genomic Approaches To Understanding Host-Virus Interactions In Shaping The Outcome Of Equine Arteritis Virus Infection, Yun Young Go

University of Kentucky Doctoral Dissertations

Equine arteritis virus (EAV) is the causal agent of equine viral arteritis, a disease of equids. During natural outbreaks of the disease, EAV can cause abortion in pregnant mares and persistent infection in stallions. Understanding how host cellular proteins interact with viral RNA and viral proteins, as well as their role in viral infection, will enable better characterization of the pathogenesis of EAV and establishment of persistent infection in stallions. Accordingly, we hypothesized that both viral factors and host genetically related factors could influence the outcome of EAV infection in horses. To test this hypothesis, we first combined contemporary molecular …

Polyamine Modulation In Alcoholism: Examination Using A Novel Screening Procedure Designed To Predict Anti-Relapse And Neuroprotective Efficacy, J. Ben Lewis

University of Kentucky Doctoral Dissertations

Alcohol dependence is a major public health concern. Despite the FDA’s approval of multiple anti-relapse drugs, relapse rates remain unacceptably high. Furthermore, cognitive deficits among chronic drinkers are evident and are suggested to contribute to relapse risk. Current evidence suggests that several critical features of alcoholism and alcohol-associated neurodegeneration are mechanistically linked to glutamatergic actions; specifically, they appear positively affected by glutamatergic inhibition, particularly inhibition via polyamine modulation of a subpopulation of n-methyl-d-aspartate receptors. The current project was designed to evaluate the performance of two putative polyamine modulators (JR-220 and CP-101,606) in a variety of screens designed …

An Examination Of Compressed Natural Gas For Use In Municipal Fleets: Would Cng Work For Lexington, Ky?, Robert Kahne

MPA/MPP/MPFM Capstone Projects

Compressed Natural Gas (CNG) is an alternative fuel source which could replace diesel as a fuel for municipal fleets. This paper seeks to examine the viability of CNG and its potential to power Lexington-Fayette Urban County Government’s fleet of waste removal trucks. This paper takes into account financial and environmental factors in determining CNG’s potential, but gives priority to financial considerations. The results of this analysis are mixed—under some future scenarios, compressed natural gas would be a favorable option for the fleet, but given other future scenarios, CNG would not be viable. In terms of environmental benefits, a switch to …

Examining Kentucky Public Schools: Gender Differences In An Index Measure Of Accountability For Academic Standards, Khin Thazin Myint

MPA/MPP/MPFM Capstone Projects

The Kentucky public education system has made progress over the past 20 years after a number of reforms beginning in 1990. A stated goal of these reforms has been not only to improve the overall performance of students in a school, but to also ensure that no student groups be allowed to fall behind. To accomplish this, the state has adopted and implemented goals that are shared with those of the federal No Child Left Behind Act, including school accountability and a goal of proficiency for all students by the year 2014. However the most recent analysis of three statewide …

Elucidating The Biochemical Wizardry Of Triterpene Metabolism In Botroycoccus Braunii, Thomas Daniel Niehaus

Theses and Dissertations--Plant and Soil Sciences

B. braunii is a green alga that has attracted attention as a potential renewable fuel source due to its high oil content and the archeological record of its unique contribution to oil and coal shales. Three extant chemotypes of B. braunii have been described, namely race A, race B, and race L, which accumulate alkadienes and alkatrienes, botryococcene and squalene and their methylated derivatives, and lycopadiene, respectively. The methylated triterpenes, particularly botryococcenes, produced by race B can be efficiently converted to high quality combustible fuels and other petrochemicals; however, botryococcene biosynthesis has remained enigmatic.

It has been suggested that botryococcene …

Molecular And Chemical Dissection Of Cellulose Biosynthesis In Plants, Darby M. Harris

Theses and Dissertations--Plant and Soil Sciences

Plant cell walls are complex structures that must not only constrain cellular turgor pressure but also allow for structural modification during the dynamic processes of cell division and anisotropic expansion. Cell walls are composed of highly glycosylated proteins and polysaccharides, including pectin, hemicellulose and cellulose. The primary cell wall polysaccharide is cellulose, a polymer composed of high molecular weight !- 1,4-glucan chains. Although cellulose is the most abundant biopolymer on Earth, there is still a lot to learn about its biosynthesis and regulation. This research began by applying a variety of analytical techniques in an attempt to understand differences in …

Structural Racism And The Law In America Today: An Introduction, William M. Wiecek

Kentucky Law Journal

No abstract provided.

Enhancements To The Modified Composite Pattern Method Of Structured Light 3d Capture, Charles Joseph Casey

University of Kentucky Doctoral Dissertations

The use of structured light illumination techniques for three-dimensional data acquisition is, in many cases, limited to stationary subjects due to the multiple pattern projections needed for depth analysis. Traditional Composite Pattern (CP) multiplexing utilizes sinusoidal modulation of individual projection patterns to allow numerous patterns to be combined into a single image. However, due to demodulation artifacts, it is often difficult to accurately recover the subject surface contour information. On the other hand, if one were to project an image consisting of many thin, identical stripes onto the surface, one could, by isolating each stripe center, recreate a very accurate …

Single-Parent Families In Bangkok, Thailand: Factors Affecting Children Living In Single-Parent Families, Waruesporn Jiumpanyarach

Theses and Dissertations--Sociology

There has been a transformation in family structure in Thailand due to the changes in economic and social structures over time. Though not recorded in census data the rise in single-parent families can be expected due to a rise in divorce rates, that have been recorded, over the past decades. However, the literature on single-parent families is limited and little is known about the experiences of single-mothers and children of singlemother families in Thailand. This study examines the factors that have major impacts on the well-being of children of single-parent families in Bangkok, Thailand.

A qualitative methodology was employed to …

Rit Gtpase Signaling Mediates Oxidative Stress Resistance And Survival Of Adult Newborn Neurons After Traumatic Brain Injury, Weikang Cai

Theses and Dissertations--Molecular and Cellular Biochemistry

The small GTPases function as molecular switches to control diverse signaling cascades. The mammalian Rit and Rin, along with Drosophila Ric, comprise an evolutionarily conserved subfamily of the Ras-related GTPases. Previous studies using cultured cell models suggested that Rit was involved in the control of cell proliferation, transformation, neuronal differentiation, morphogenesis, and cell survival, but the principal physiological function of Rit remained uncharacterized.

To address this outstanding question, we employed a genetic approach, engineering a Rit knockout mouse. Using this animal model, we demonstrate a central role of Rit in governing cell survival in a p38-dependent fashion. Primary mouse embryonic …

Intrapsychic Predictors Of Professional Quality Of Life: Mindfulness, Empathy, And Emotional Separation, Jacky T. Thomas

University of Kentucky Doctoral Dissertations

A growing literature documents the inherently stressful nature of working with persons who are suffering or traumatized, and the potential for the development of stress disorders among social workers and other helpers. Previous studies of compassion fatigue and burnout have provided important information about professional and workplace variables that might influence risk, but little attention has been given to studying intrapersonal skills/abilities that might reduce risk and/or increase resilience and work satisfaction among helping professionals. This exploratory study asked whether levels of mindfulness, empathy, and emotional separation would influence professional quality of life, including compassion fatigue, burnout, and compassion satisfaction. …

The Role Of Co-Infection In The Spread Of Hiv In Sub-Saharan Africa, Diego Fernando Cuadros

Theses and Dissertations--Biology

The cause of the high HIV prevalence in sub-Saharan Africa is incompletely understood, with heterosexual penile-vaginal transmission proposed as the main mechanism. Heterosexual HIV transmission has a very low probability; further, a single estimation of heterosexual probability of HIV transmission fails to reproduce the variation associated with important biological cofactors. In particular, studies of HIV incidence suggest that co-infection with other infectious diseases influence the HIV transmission, and therefore might substantially vary the pattern of the spread of the infection. To assess the effect of co-infection on the spread of HIV, I developed and analyzed several mathematical and statistical models …

Simulation And Control Of A Quadrotor Unmanned Aerial Vehicle, Michael David Schmidt

University of Kentucky Master's Theses

The ANGEL project (Aerial Network Guided Electronic Lookout) takes a systems engineering approach to the design, development, testing and implementation of a quadrotor unmanned aerial vehicle. Many current research endeavors into the field of quadrotors for use as unmanned vehicles do not utilize the broad systems approach to design and implementation. These other projects use pre-fabricated quadrotor platforms and a series of external sensors in a mock environment that is unfeasible for real world use. The ANGEL system was designed specifically for use in a combat theater where robustness and ease of control are paramount. A complete simulation model of …

Healing Healthcare Design For Adolescent Patients: Promoting Holistic Quality Of Life, Eun Young Kim

University of Kentucky Master's Theses

This study examined environmental preferences for adolescent patients in hospital patient rooms and activity rooms to determine age-appropriate healing design elements. The health-related quality of life (HRQOL) concept was adapted to this study as a theoretical framework. In order to develop an age-appropriate healing design, a comprehensive understanding of adolescents‘ cognitive developmental stages and their expectations in hospital settings needs to be recognized. Thirty-two adolescent outpatients aged 15 to 18 participated in the survey. Data collection consisted of three different instruments: Emotional state survey with demographics, Photo analysis with semantic differentials, and environmental preference value survey. Comfort, sadness and stress …