Digital Commons Network^™

An Evaluation Of Physiological And Behavioral Indicators Of Postpartum Diseases And Heat Stress In Dairy Cows, Amanda Renee Lee

Theses and Dissertations--Animal and Food Sciences

Precision dairy monitoring technologies can be used to monitor changes in physiology and behavior associated with transition period postpartum diseases and heat stress. Research Objective One was to evaluate how traditional visual examination, body condition, and locomotion with and without blood, milk, and urine variables and precision dairy monitoring technologies determine variable association with hyperketonemia, metritis, mastitis, hypocalcemia and retained placenta,. This was accomplished by monitoring cows 2 weeks before calving to 3 weeks after calving for any postpartum diseases using daily visual examination and automatically detected variables including activity, milk yield, milk components, lying behavior, feeding behavior, rumination time, …

The Effects Of Housing On Dairy Cow Comfort, Immune Function, Stress, Productivity, And Milk Quality, Matthew Richard Borchers

Theses and Dissertations--Animal and Food Sciences

Mastitis and milk quality affect every dairy farmer across the globe. Sand bedded freestalls are the industry standard for cow comfort, welfare, and the control of environmental mastitis. Compost bedded packs may be a viable alternative to the sand bedded freestall.

Compost bedded packs are maintained at a consistent level of moisture, nutrients, and aeration to favor compost microorganisms. Greater bacteria counts in bedding have traditionally been associated with increased mastitis rates and mastitis pathogens can be found in the pack and on the teats of cattle housed in even well managed compost bedded pack barns. In spite of this, …

Whole-Body Protein Metabolism In Mature And Growing Horses Receiving Predominantly Forage Diets, Sophie A. Stratton

Theses and Dissertations--Animal and Food Sciences

There has been limited investigation as to whether a predominantly forage-based diet can provide adequate amounts of limiting amino acids (AA) to horses. The first objective was to determine if AA supplementation of AA believed to be limiting to protein synthesis in forage-based diets would affect measures of whole-body protein metabolism in sedentary mature horses. The effect of forage type (timothy or alfalfa) and AA supplementation (lysine, threonine or histidine) on plasma urea nitrogen (PUN) and AA concentrations and measures of whole-body phenylalanine kinetics were evaluated. There was no effect of either forage type or AA supplement on rates of …

Older Adult Men’S Emotional Bonds With Their Dogs, Ranell L. Mueller

Theses and Dissertations--Gerontology

Knowledge of the deeper meanings of attachment to companion animals is limited, particularly in terms of older adults. This study employed a modified grounded theory method, a phenomenological lens and a life course perspective to gather and analyze data garnered from individual interviews and panel discussions in order to investigate the multiple dimensions of older adult men’s relationships with their companion animal dogs. Individual audio-recorded in-depth interviews and repeated panel discussions with a sub-group of the participants, convened as a panel over a three-month period, explored behavioral and emotional manifestations of attachment and the emotional bond to their companion animal …

Multivariate Analysis To Identify Potential Biomarkers For Prognosis And Treatment Resistance In Head And Neck Cancer Patients, Christina Ann Wicker

Theses and Dissertations--Toxicology and Cancer Biology

It is estimated that nearly 50,000 individuals in the United States will be diagnosed with head and neck cancer in 2017 (American Cancer Society www.cancer.org). Ninety percent of oral cancers are head and neck squamous cell carcinoma (HNSCC). Major obstacles in the treatment of HNSCC are recurrence and treatment resistance, which contributes to increased mortality. Therefore, there is increased need to determine genetic alterations in HNSCC that may be ideal novel drug targets, and biomarkers to improve diagnostic and prognostic testing.

Abnormal localization and overexpression of base excision repair protein and transcriptional regulator Apurinic/Apyrimidic endonuclease (APE1) has been associated with …

Role Of Oxidized Extracellular Vesicles As Early Biomarkers And Inflammatory Mediators In Chemotherapy-Induced Normal Tissue Injury, Chontida Yarana

Theses and Dissertations--Toxicology and Cancer Biology

Significant advances in the efficacy of cancer therapy have been accompanied by an escalation of side effects that result from therapy-induced injury to normal tissues. Patients with high grade cancer or metastasis are often treated with chemotherapy, 50% of which are associated with reactive oxygen species generation and cellular oxidative stress. Heart is the normal tissue most susceptible to chemotherapy-induced oxidative stress and heart disease is the most common leading cause of death in cancer survivors. However, early and sensitive biomarkers to identify heart disease are still lacking. Extracellular vesicles (EVs) are released from cells during oxidative stress and send …

Understanding Haitian Women’S Health Care In Immokalee, Florida, Usa, Michele Leigh Flippo Bolduc

Theses and Dissertations--Geography

This social science research project takes a critical approach to understanding the health of a population by using the health care system as an entry point through which we can see how large-scale social processes produce a particular health care landscape in the rural, im/migrant farmworker community of Immokalee, Florida, USA. Using a multi-scalar analysis of health care, I investigate how anti-immigrant legislation and neoliberal economics influence the experience of health care for health care providers and Haitian im/migrant women navigating these processes. First, I argue that anti-immigrant and pro-market discourses have been successful in limiting the accessibility to health …

Accounting For Spatial Autocorrelation In Modeling The Distribution Of Water Quality Variables, Lorrayne Miralha

Theses and Dissertations--Geography

Several studies in hydrology have reported differences in outcomes between models in which spatial autocorrelation (SAC) is accounted for and those in which SAC is not. However, the capacity to predict the magnitude of such differences is still ambiguous. In this thesis, I hypothesized that SAC, inherently possessed by a response variable, influences spatial modeling outcomes. I selected ten watersheds in the USA and analyzed them to determine whether water quality variables with higher Moran’s I values undergo greater increases in the coefficient of determination (R²) and greater decreases in residual SAC (rSAC) after spatial modeling. I compared non-spatial ordinary …

The Impacts Of Honey Bee Queen Stress On Worker Behavior And Health, Sarah R. Preston

Theses and Dissertations--Entomology

Pesticides, poor nutrition, parasites and diseases work synergistically to contribute to the decline of the honey bee. Heritable sub-lethal behavior/immune effects may also contribute to the decline. Maternal stress is a common source of heritable immune/behavior deficits in many species. A stressed honey bee queen has the potential to pass such deficits on to worker bees. Using a repeated measures design, this study will determine whether the health of worker bee is reduced by a cold stress on the queen by analyzing egg hatch rate and protein content, emergence rate, and adult aggression and immune function for offspring laid before …

There’S Nowhere To Hide: Artificial Intelligence And Privacy In The Fourth Industrial Revolution, Sarah Denton, Eleonore Pauwels, Yujia He, Walter G. Johnson

Patterson School of Diplomacy and International Commerce Faculty Publications

At its core, artificial intelligence (AI) optimizes data. Machine-learning algorithms, one component of AI, are trained using massive datasets curated by humans to predict various aspects of our daily lives. Such predictive intelligence could be a positive force amplified by continued decentralization of the technology. Or, ubiquitous cognition and surveillance could be a disruptive force amplified by the unregulated proliferation of AI technologies. Yet, though we will certainly see more and more AI systems being integrated into every facet of our infrastructures, homes, and bodies, the proliferation of AI technology isn’t the problem. A privacy-security quagmire arises from the interconnectivity …

Epidemiology Of Young Adult Renal Cell Carcinoma In Kentucky: Incidence, Clinicopathologic Features, And Cancer-Specific Survival, Jason R. Bylund

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Introduction:

Renal cell carcinoma (RCC) is an important cause of morbidity and mortality in our country. Previously reported data suggest that the overall incidence of RCC continues to rise and may be accelerating even faster among younger adult patients.

Methods:

Data for analysis included the combined NPCR-SEER USCS public use database and the Kentucky Cancer Registry dataset. Incidence rates and trends by age group and location were calculated and tested. Clinicopathologic features of disease among Kentucky cases were analyzed by age group. Cancer-specific survival (CSS) analysis was performed, along with Cox proportional hazards regression to test for association of younger …

An Assessment Of Agreement In Detection Methods For Histophilus Somni In Bovine Lungs, Laura Michelle Arnold

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Objectives: To examine the inter-laboratory agreement between multiplex realtime quantitative polymerase chain reaction (qPCR) assay results and aerobic culture results on bovine lung samples for detection of Histophilus somni (HS), and to assess associations of laboratory-derived factors on test agreement.

Methods: A survey of records from the University of Kentucky Veterinary Diagnostic Laboratory (UKVDL) was conducted to evaluate test results from grossly pneumonic bovine lung samples submitted during the period April 1, 2015 through August 31, 2018. Cohen’s kappa coefficient with 95% confidence interval (CI) was calculated to describe the extent of agreement. Animal, environmental and laboratory factors were examined …

The Assessment And Utilization Of Patients’ Self-Efficacy For Exercise During Rehabilitation, Kelsey J. Picha

Theses and Dissertations--Rehabilitation Sciences

Patient adherence to in-clinic rehabilitation is between 30-70% and even lower for home exercise programs (HEPs). Barriers to patient adherence have been identified and include but are not limited to anxiety, depression, lack of positive feedback, lack of social support, lack of time, low levels of physical activity at baseline, pain during exercise, and low self-efficacy. As clinicians prescribing rehabilitation may not be able to influence all of the identified barriers, they may positively influence others. Self-efficacy, or an individual’s belief in his/her ability to successfully complete a task, is a patient barrier that may be addressed by a clinician …

Tissues And Trauma: Pain Neuroscience Education For Veterans With Post-Traumatic Stress And Low Back Pain, Timothy Mark Benedict

Theses and Dissertations--Rehabilitation Sciences

Low back pain (LBP) is the top reason for Soldiers to seek medical care and one of the top reasons to be medically discharged. Mental health problems and psychosocial stressors have been increasing in Soldiers and are also top causes for medical discharge. Dysregulated stress has contributed to many Soldiers and Veterans to develop chronic LBP as well as mental health disorders like post-traumatic stress disorder (PTSD). Research suggests that psychosocial characteristics, as opposed to physical factors or tissue health, contribute to chronic pain the most. Focusing entirely on tissues for individuals seeking care for LBP can increase disability and …

Vacating Adult Adoptions Post-Obergefell, Sarah A. Quarles

Kentucky Law Journal

No abstract provided.

What Is Sexual Orientation?, Mary Ziegler

Kentucky Law Journal

At a time when the Supreme Court seems closer than ever before to treating sexual orientation as a suspect classification, consideration of the legal definition of sexual orientation is both timely and important. The Court’s 2015 decision in Obergefell recognizes two guideposts for defining sexual orientation: its immutability and normalcy. While other scholars offer rich and nuanced accounts of the fight for gay, lesbian, transgender, and bisexual rights, they do not fully analyze the history of sexual orientation as a legal category. This Article closes that gap, illuminating the hidden costs of the definition of sexual orientation that Obergefell endorses. …

Does The Foundational Model Of Anatomy Ontology Provide A Knowledge Base For Learning And Assessment In Anatomy Education?, Melissa D. Clarkson, Mark E. Whipple

Institute for Biomedical Informatics Faculty Publications

Throughout the development of the Foundational Model of Anatomy (FMA) ontology, one of the use cases put forth has been anatomy education. In this work, we examine which types of knowledge taught to anatomy students can be supported by the FMA knowledge base. We first categorize types of anatomical knowledge, then express these patterns in the form “Given ____, state ____”. Each of the 33 patterns was evaluated for whether this type of knowledge is compatible with the modeling and scope of the FMA.

Developing Graphic Libraries To Accompany The Craniofacial Human Ontology, Melissa D. Clarkson

Institute for Biomedical Informatics Faculty Publications

I describe the development of two graphic libraries to accompany parts of the Craniofacial Human Ontology. One library depicts phenotypes of cleft lip. The other represents development of the human head between 4 and 8 weeks of gestation.

Validating Spray Coverage Rate Using Liquid Mass On A Spray Card, Michael P. Sama, Austin M. Weiss, Emma K. Benedict

Biosystems and Agricultural Engineering Faculty Publications

Validation of agricultural sprayers is important for quantifying as-applied coverage rates under field conditions. The complexity of modern sprayer control systems presents a challenge for precise field validation due to the use of nozzle control technologies, such as pulse width modulation, to meter chemical flow rates at individual nozzles. Non-uniform flow over time may result in local variations at high spatial resolutions that are ignored when estimating as-applied coverage rates across a field. The purpose of this study was to test several methods for estimating the mass of water applied to a water-sensitive paper spray card target using steady-state and …

Application Of Acoustic Emission And Machine Learning To Detect Codling Moth Infested Apples, Mengxing Li, Nader Ekramirad, Ahmed Rady, Akinbode A. Adedeji

Biosystems and Agricultural Engineering Faculty Publications

Incidence of codling moth (CM) (Cydia pomonella L.) infestation in apples has been a major concern in North America for decades. CM larvae bore deep into the fruit, making it unmarketable. An effective noninvasive method to detect larvae-infested apples is necessary to ensure that apples are CM-free in post-harvest processing. In this study, a novel approach using an acoustic emission (AE) system and subsequent machine learning methods was applied to classify larvae-infested apples from intact apples. 'GoldRush‘ apples were infested with CM neonates and stored at the same conditions as intact apples. The AE system was used to collect …

Hydraulic Properties Of Baled Switchgrass And Miscanthus, Drew F. Schiavone, Michael D. Montross

Biosystems and Agricultural Engineering Faculty Publications

This study characterized the hydraulic properties within baled switchgrass ( Panicum virgatum L., variety Alamo) and miscanthus (Miscanthus x giganteus), thereby enabling a better understanding of moisture changes in baled biomass during on-farm storage and/or high-solids bioconversion. Fully saturated bales were drained by gravity, and the moisture content was determined over 60 h. The average initial moisture content ranged between 55.9% and 71.9% (w.b.) for switchgrass and between 60.5% and 73.9% (w.b.) for miscanthus bales depending on the dry bale density. As the bale drained by gravity, rapid leaching of water was observed within the first 0.1 h, …

Improving In-Stream Nutrient Routines In Water Quality Models Using Stable Isotope Tracers: A Review And Synthesis, Alexandria K. Jensen, William I. Ford, James F. Fox, Admin Husic

Biosystems and Agricultural Engineering Faculty Publications

Water quality models serve as an economically feasible alternative to quantify fluxes of nutrient pollution and to simulate effective mitigation strategies; however, their applicability is often questioned due to broad uncertainties in model structure and parameterization, leading to uncertain outputs. We argue that reduction of uncertainty is partially achieved by integrating stable isotope data streams within the water quality model architecture. This article outlines the use of stable isotopes as a response variable within water quality models to improve the model boundary conditions associated with nutrient source provenance, constrain model parameterization, and elucidate shortcomings in the model structure. To assist …

Genotype-Phenotype Study In Patients With Valosin-Containing Protein Mutations Associated With Multisystem Proteinopathy, Ebaa Al-Obeidi, Sejad Al-Tahan, Abhilasha Surampalli, Namita Goyal, Annabel K. Wang, Andreas Hermann, Molly Omizo, Charles D. Smith, Tahseen Mozaffar, Virginia Kimonis

Neurology Faculty Publications

Mutations in valosin‐containing protein (VCP), an ATPase involved in protein degradation and autophagy, cause VCP disease, a progressive autosomal dominant adult onset multisystem proteinopathy. The goal of this study is to examine if phenotypic differences in this disorder could be explained by the specific gene mutations. We therefore studied 231 individuals (118 males and 113 females) from 36 families carrying 15 different VCP mutations. We analyzed the correlation between the different mutations and prevalence, age of onset and severity of myopathy, Paget's disease of bone (PDB), and frontotemporal dementia (FTD), and other comorbidities. Myopathy, PDB and FTD was present in …

Autonomic Dysreflexia After Spinal Cord Injury: Systemic Pathophysiology And Methods Of Management, Khalid C. Eldahan, Alexander G. Rabchevsky

Physiology Faculty Publications

Traumatic spinal cord injury (SCI) has widespread physiological effects beyond the disruption of sensory and motor function, notably the loss of normal autonomic and cardiovascular control. Injury at or above the sixth thoracic spinal cord segment segregates critical spinal sympathetic neurons from supraspinal modulation which can result in a syndrome known as autonomic dysreflexia (AD). AD is defined as episodic hypertension and concomitant baroreflex-mediated bradycardia initiated by unmodulated sympathetic reflexes in the decentralized cord. This condition is often triggered by noxious yet unperceived visceral or somatic stimuli below the injury level and if severe enough can require immediate medical attention. …

Serum Tumor Necrosis Factor-Alpha Associates With Myocardial Oxygen Demand And Exercise Tolerance In Postmenopausal Women, Stephen J. Carter, David R. Bryan, William H. Neumeier, Stephen P. Glasser, Gary R. Hunter

Internal Medicine Faculty Publications

The functional implications of serum tumor necrosis factor-alpha (TNF-α), a marker of oxidative stress, on hemodynamic parameters at rest and during physical exertion are unclear. The aims of this investigation were to examine the independent associations of TNF-α on myocardial oxygen demand at rest and during submaximal exercise, while also evaluating the association of TNF-α on exercise tolerance. Forty, postmenopausal women, provided blood samples and completed a modified-Balke protocol to measure maximal oxygen uptake (VO_2max). Large artery compliance was measured by pulse contour analyses while rate-pressure product (RPP), an index of myocardial oxygen demand, was measured at rest …

Targeting The Cellular Redox Environment: A Novel Approach For The Treatment Of Hematopoietic Neoplasms, Dustin W. Carroll

Theses and Dissertations--Toxicology and Cancer Biology

Hematopoietic stem cells (HSCs) that function to maintain the hematopoietic compartment through self-renewal and differentiation capacities, as well as their downstream progeny, are susceptible to transformation resulting in the generation of the leukemic stem cell (LSC). Chief in the factors that control HSC regulation and protection of the HSC compartment is the cellular redox environment. Deregulation of the Hematopoietic Stem/Progenitor Cell (HSPC) redox environment results in loss of HSPC function and exhaustion. The characteristic developments of HSPC exhaustion via exposure to redox stress closely mirror phenotypic traits of hematopoietic malignancies, presenting the HSPC/LSC redox environment as a potential therapeutic target. …

A Novel Role For The Tumor-Suppressor Par-4 In Regulation Of Adipogenesis And Obesity, James Sledziona

Theses and Dissertations--Toxicology and Cancer Biology

Prostate Apoptosis Response-4 (Par-4) is a conserved and ubiquitous tumor-suppressor factor which can selectively induce apoptosis in tumor cells, while leaving normal cells unaffected. While Par-4 is well established as a tumor-suppressor, there have yet been no formal investigations as to whether it has a physiologic role in normal tissues.

Early observations of Par-4 knockout mouse lines yielded that the adult mice displayed significant weight gain and fat accumulation compared to their wild-type counterparts while on a conventional chow diet. Interestingly, obese mouse and human subjects were found to exhibit reduced expression of Par-4 in adipose tissue as well as …

Impact Of Physico-Chemical Properties Of Manufactured Nanomaterials On Plant Uptake And Trophic Transfer, Jieran Li

Theses and Dissertations--Toxicology and Cancer Biology

Large quantities of manufactured nanomaterials (MNM) are released into the environment by human activity each year. The entry of MNM into the terrestrial food webs, which has the potential for far-reaching impacts, begins with the uptake by plant species from the soil. These processes can be affected by MNM physico-chemical properties such as size, chemical composition, surface charge, etc., of which our knowledge is still incomplete. To bridge some of the gaps in our understanding of these processes and, specifically, to determine whether the physico-chemical properties of the MNM are predictive of their behavior in terrestrial food chains, we conducted …

Impact Of Health Insurance And Sociodemographic Characteristics On Survival For Women Diagnosed With Breast Cancer In Kentucky, Pramila Rai

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Introduction Objective of the study was to explore the impact of health insurance and socio-demographic factors on survival for breast cancer patients in Kentucky. Breast cancer is the most prevalent cancer among women in the US. Breast cancer survival is affected by various factors including health insurance, residence, age, race, geographical distance, income.

Methods The data were obtained from Kentucky Cancer Registry and included 47,128 women diagnosed with primary breast cancer between 2000 to 2014. The relationship of health insurance and other socio-demographic factors was analyzed using Cox regression.

ResultsThe overall five-year survival proportion was 0.97, 0.76 and 0.71 …

Implementing The National Diabetes Prevention Program In Mason County, Kentucky, Cassandra Yelton

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

The National Diabetes Prevention Program (DPP) is sponsored by the Centers for Disease Control and Prevention (CDC). The program’s goal is to reduce the incidence of diabetes through dietary modification and physical activity. On one hand, the DPP is currently offered by various organizations through the country. On the other hand, the DPP is mostly unavailable in rural areas, especially the Diabetes Belt. The Diabetes Belt is in the eastern Appalachian region of the United States. People who live in this area of the country are more likely to develop diabetes than those who do not. Mason County, Kentucky has …