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Survey Of Tetraploid And Diploid Perennial Pastures In The Waikato For Number Of Spores Produced By The Fungus Pithomyces Chartarum, J. P. J. Eerens, W. W. Nichol, J. Waller, J. M. Mellsop, M. R. Trolove, M. G. Norriss

IGC Proceedings (1977-2023)

Facial eczema (FE) is a disease of livestock, caused by a toxin released into the bloodstream after digestion of spores of Pithomyces chartarum, a fungus residing in necrotic plant material in the base of pastures (di Menna & Bailey, 1973). Spore numbers tend to be highest in warm, humid conditions, where high post grazing residuals have lead to a build up of necrotic plant material. Tetraploid perennial ryegrass pastures tend to be more palatable, and with lower post gazing residuals, than equivalent diploid pastures; thus we hypothesised that spore numbers would be lower in tetraploid pastures. A survey of …

Perennial Ryegrass Variety Differences In Nutritive Value Characteristics, T. J. Gilliland, R. E. Agnew, A. M. Fearson, F. E. A. Wilson

IGC Proceedings (1977-2023)

Animal grazing performance at grass is predominately determined by herbage intake rates, with high yielding dairy cows requiring up to 20 kg/d DM within a limited grazing time (Gibb, 1998). Grass nutritional factors such as seasonal patterns in digestibility and water-soluble carbohydrate levels have been linked to animal productivity (Davies et al., 1991), while sward surface height, herbage mass, bulk density and green leaf mass have been shown to promote high grazing intake (Barrett et al., 2001). Furthermore, fatty acid profiles have been shown to improve the unsaturated fatty acid composition of milk, with potential human health benefits …

Optimising Financial Return From Grazing In Temperate Pastures, P. Dillon, J. R. Roche, L. Shalloo, B. Horan

IGC Proceedings (1977-2023)

Key Points

1. The increased interest in pasture-based systems of milk production in recent years has been largely generated through lower product prices and rising costs of production.

2. Pasture based systems of milk production decrease unit production costs, through lower feed and labour expenses, and reduced capital investment.

3. Systems utilising grazed pasture will be optimised in regions where pasture production potential is high, variability in seasonal pasture supply and quality is low, manufacturing milk accounts for a large proportion of total production, and where large areas of land are available at relatively low cost.

4. Pasture based systems may allow greater global …

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Challenges And Opportunities For Animal Production From Temperate Pastures, D. A. Clark

IGC Proceedings (1977-2023)

Key points

1. Temperate pastures offer a major opportunity to reduce the feed costs associated with ruminant production.

2. Pastures offer unique opportunities for producing high value components in feedstuffs that are beneficial to human health.

3. The increased use of pasture will not automatically lead to improved environmental outcomes – difficult challenges exist in reducing nitrogen and greenhouse gas pollution.

4. Grazed pasture systems offer advantages in animal welfare, provided management avoids the problems associated with climatic extremes, and the toxins associated with some pastures.

5. To remain competitive with total mixed ration systems, and environmentally acceptable, pastures with higher intake characteristics that allow …

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Decision Support For Temperate Grasslands: Challenges And Pitfalls, J. R. Donnelly, L. Salmon, R. D. H. Cohen, Zl. Liu, Xp. Xin

IGC Proceedings (1977-2023)

Key points

1. Successful adoption of decision support tools (DS tools) to address grassland management issues requires careful attention in design to ensure ease-of-use, accuracy in prediction and the flexibility to simulate actual practices.

2. DS tools must handle spatial variability and where possible include facilities for automatic sourcing of essential information for initialisation.

3. Advances in the development of DS tools will depend on resolution of scientific issues in grassland biology including investment in dedicated experiments to determine parameter values for model equations.

4. The use of mechanistic models, the integration of remote sensing technology and cooperation between research groups to develop modular …

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Modelling Of Herbage Intake And Milk Production By Grazing Dairy Cows, Rémy Delagarde, M. O'Donovan

IGC Proceedings (1977-2023)

Key-points

1. Models predicting intake and performance of grazing ruminants from animal, sward, grazing and supplements characteristics are rare, but they are now included in several decision support systems (DSS).

2. An evaluation of the performance and accuracy of published models are rarely undertaken by their authors, but this is proposed in this paper.

3. There is still a need for experimental research but also for the development of generic and dynamic models to predict intake and performance over a wide range of grazing conditions.

Grass Growth Modelling: To Increase Understanding And Aid Decision Making On-Farm, P. D. Barrett, A. S. Laidlaw

IGC Proceedings (1977-2023)

Key points

1. Crop and grass growth models have been developed over the last 50 years, or so, but general appreciation of their benefits and potential has been recognised only relatively recently. The most popular application of grass growth models has traditionally been for knowledge understanding.

2. There is growing awareness of the potential of models in decision support systems (DSS) applications to aid pasture management and grassland budgeting on dairy farms.

3. Although some models have been developed for DSS, their widespread uptake in industry has been slow; challenges still exist which need to be addressed in order to improve their precision …

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Genetic Characteristics Required In Dairy And Beef Cattle For Temperate Grazing Systems, F. Buckley, C. Holmes, M. G. Keane

IGC Proceedings (1977-2023)

Key points

1. Only about 10% of the world’s milk is produced from grazing systems. Consequently the majority of dairy cattle have not been selected under grazing, nor on seasonal systems. This is not true for beef cattle, for which the majority, especially the dams, are managed under seasonal grazing systems.

2. In grazing systems daily feed intake is limited to lower levels than are achievable on concentrate plus conserved forage rations. Consequently, cows most suited to grazing environments are likely to have a lower genetic potential for milk production than cows selected in high concentrate systems, to minimise their relative energy …

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Overview Of Animal Production From Pastures In Ireland, M. J. Drennan, A. F. Carson, S. Crosse

IGC Proceedings (1977-2023)

Key points

The importance of grassland to agriculture in Ireland is indicated by the fact that:

1. Sixty percent of agricultural output is from grassland as cattle, milk and sheep products.
2. Over 90% of the total farmed area is in grass.
3. Livestock are almost entirely dependent on grazed grass for 200 to 235 days of the year.
4. Grass conserved as silage is the main source of fodder in winter.
5. To improve competitiveness changes are continuously taking place, which include:

• increased suckler herd size and a movement to late maturing continental cattle breeds;
• movement in the dairy herd towards Holstein with increased …

The Archives And You: Why Your Memorabilia Matters, Ruth E. Bryan

Library Presentations

Join Ruth E. Bryan, University Archivist at the University of Kentucky Libraries Special Collections Research Center, as she describes what archives are and how your memorabilia can contribute to historical research and scholarship.

Kentucky Annual Economic Report 2023, Michael Childress, Michael W. Clark, James P. Ziliak, Simon Sheather

Kentucky Annual Economic Report

This report is one of the important ways that the Center for Business and Economic Research fulfills its mission to examine various aspects of Kentucky’s economy as directed by the Kentucky Revised Statutes (KRS 164.738). The analysis and data presented here cover a variety of topics that range from a discussion of Kentucky’s current economic climate to a broad presentation of factors affecting the economy.

Higher Concentrations Of Microplastics In Runoff From Biosolid-Amended Croplands Than Manure-Amended Croplands, Nasrin Naderi Beni, Shahab Karimifard, John Gilley, Tiffany L. Messer, Amy Schmidt, Shannon Bartelt-Hunt

UK CARES Faculty Publications

Land-applied municipal biosolids, produced from municipal wastewater treatment sludge, contributes to microplastics contamination in agroecosystems. The impacts of biosolids on microplastic concentrations in agricultural soil have been previously investigated, however, the potential for microplastics transport from biosolid-amended croplands has not been previously quantified. In this study, manure and biosolids were applied to field plots, runoff was collected following natural precipitation events and the potential of bacterial biofilm to grow on different microplastic morphologies was investigated. Higher concentrations of microplastics were detected in runoff from plots with land-applied biosolid in comparison with manure-amended and control plots. Fibers and fragments were the …

Forage News [2023-02], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

No abstract provided.

Novel Smad3 Variant Identified In A Patient With Familial Aortopathy Modeled Using A Zebrafish Embryo Assay, Mary Burchett Sheppard, Jeffrey D. Smith, Lisa L. Bergmann, Jakub Konrad Famulski

Saha Cardiovascular Research Center Faculty Publications

In human, pathogenic variants in smad3 are one cause of familial aortopathy. We describe a novel SMAD3 variant of unknown significance (VUS), V244F, in a patient who presented with aortic root dilation, right coronary artery ectasia, abdominal aortic aneurysm, right vertebral artery atresia, and cavernoma. Determination of variant pathogenicity impacted multiple aspects of the patient’s care, including the most appropriate surgical threshold for which to recommend a valve-sparing aortic root replacement. To determine whether the newly identified SMAD3 variant, and whether SMAD3 induced aortopathy in general, can be assayed in a zebrafish embryo model, we injected smad3a mRNA into Tg[kdrl:mCherry] …

Aortic Stress Activates An Adaptive Program In Thoracic Aortic Smooth Muscle Cells That Maintains Aortic Strength And Protects Against Aneurysm And Dissection In Mice, Chen Zhang, Yanming Li, Abhijit Chakraborty, Yang Li, Kimberly R. Rebello, Pingping Ren, Wei Luo, Lin Zhang, Hong S. Lu, Lisa A. Cassis, Joseph S. Coselli, Alan Daugherty, Scott A. Lemaire, Ying H. Shen

Saha Cardiovascular Research Center Faculty Publications

BACKGROUND: When aortic cells are under stress, such as increased hemodynamic pressure, they adapt to the environment by modifying their functions, allowing the aorta to maintain its strength. To understand the regulation of this adaptive response, we examined transcriptomic and epigenomic programs in aortic smooth muscle cells (SMCs) during the adaptive response to AngII (angiotensin II) infusion and determined its importance in protecting against aortic aneurysm and dissection (AAD).

METHODS: We performed single-cell RNA sequencing and single-cell sequencing assay for transposase-accessible chromatin (scATAC-seq) analyses in a mouse model of sporadic AAD induced by AngII infusion. We also examined the direct …

Adolescent- And Adult-Initiated Alcohol Exposure In Mice Differentially Promotes Tumorigenesis And Metastasis Of Breast Cancer, Mei Xu, Hui Li, Danlei Chen, Huaxun Wu, Wen Wen, Hong Xu, Jacqueline Frank, Gang Chen, Jia Luo

Markey Cancer Center Faculty Publications

Background: Alcohol exposure increases the risk of breast cancer. Alcohol consumption by adolescents is a serious social and public health issue. This study investigated the impact of adolescent alcohol consumption on mammary tumorigenesis and progression and compared it to that of adult alcohol exposure in animal models. Methods: Female adolescent (5 weeks) and adult (8 weeks) MMTV-Wnt1 mice were exposed to alcohol either chronically or acutely. For chronic alcohol exposure, animals were fed a liquid diet containing 6.7% ethanol for 23 weeks. For acute exposure, animals were treated with ethanol (2.5 g/kg, 25% w/v) via intraperitoneal (IP) injection for 15 …

Bilirubin Nanoparticle Treatment In Obese Mice Inhibits Hepatic Ceramide Production And Remodels Liver Fat Content, Zachary A. Kipp, Genesee J. Martinez, Evelyn A. Bates, Agil B. Maharramov, Robert M. Flight, Hunter N. B. Moseley, Andrew J. Morris, David E. Stec, Terry D. Hinds Jr.

Markey Cancer Center Faculty Publications

Studies have indicated that increasing plasma bilirubin levels might be useful for preventing and treating hepatic lipid accumulation that occurs with metabolic diseases such as obesity and diabetes. We have previously demonstrated that mice with hyperbilirubinemia had significantly less lipid accumulation in a diet-induced non-alcoholic fatty liver disease (NAFLD) model. However, bilirubin’s effects on individual lipid species are currently unknown. Therefore, we used liquid chromatography-mass spectroscopy (LC-MS) to determine the hepatic lipid composition of obese mice with NAFLD treated with bilirubin nanoparticles or vehicle control. We placed the mice on a high-fat diet (HFD) for 24 weeks and then treated …

Contouring Quality Assurance Methodology Based On Multiple Geometric Features Against Deep Learning Auto-Segmentation, Jingwei Duan, Mark E. Bernard, James Castle, Xue Feng, Chi Wang, Mark C. Kenamond, Quan Chen

Markey Cancer Center Faculty Publications

Background: Contouring error is one of the top failure modes in radiation treatment. Multiple efforts have been made to develop tools to automatically detect segmentation errors.Deep learning-based auto-segmentation (DLAS) has been used as a baseline for flagging manual segmentation errors, but those efforts are limited to using only one or two contour comparison metrics.

Purpose: The purpose of this research is to develop an improved contouring quality assurance system to identify and flag manual contouring errors.

Methods and materials: DLAS contours were used as a reference to compare with manually segmented contours. A total of 27 geometric agreement metrics were …

Ifn-Γ Production By Brain-Resident Cells Activates Cerebral Mrna Expression Of A Wide Spectrum Of Molecules Critical For Both Innate And T Cell-Mediated Protective Immunity To Control Reactivation Of Chronic Infection With Toxoplasma Gondii, Yasuhiro Suzuki, Jenny Lutshumba, Kuey Chu Chen, Mohamed H. Abdelaziz, Qila Sa, Eri Ochiai

Markey Cancer Center Faculty Publications

We previously demonstrated that brain-resident cells produce IFN-g in response to reactivation of cerebral infection with Toxoplasma gondii. To obtain an overall landscape view of the effects of IFN-g from brain-resident cells on the cerebral protective immunity, in the present study we employed NanoString nCounter assay and quantified mRNA levels for 734 genes in myeloid immunity in the brains of T and B cell-deficient, bone marrow chimeric mice with and without IFN-g production by brain-resident cells in response to reactivation of cerebral T. gondii infection. Our study revealed that IFN-g produced by brain-resident cells amplified mRNA expression for the molecules …

Recombinant Protein Vaccines Formulated With Enantio-Specific Cationic Lipid R-Dotap Induce Protective Cellular And Antibody-Mediated Immune Responses In Mice, Siva Gandhapudi, Hua Shi, Martin Ward, John Peyton Bush, Margarita G. Avdiushko, Karuna Sundarapandiyan, Lauren V. Wood, Mania Dorrani, Afsheen Fatima, Joe Dervan, Frank Bedu-Addo, Greg Conn, Ted M. Ross, Jerold Woodward

Markey Cancer Center Faculty Publications

Adjuvants are essential components of subunit vaccines added to enhance immune responses to antigens through immunomodulation. Very few adjuvants have been approved for human use by regulatory agencies due to safety concerns. Current subunit vaccine adjuvants approved for human use are very effective in promoting humoral immune responses but are less effective at promoting T-cell immunity. In this study, we evaluated a novel pure enantio-specific cationic lipid 1,2-dioleoyl-3-trimethylammonium-propane (R-DOTAP) as an immunomodulator for subunit vaccines capable of inducing both humoral- and cellular-mediated immunity. Using recombinant protein antigens derived from SARS-CoV2 spike or novel computationally optimized broadly reactive influenza antigen (COBRA) …

Production And Characterization Of Monoclonal Antibodies To Xenopus Proteins, Brett Horr, Ryan Kurtz, Ankit Pandey, Benjamin G. Hoffstrom, Elizabeth Schock, Carole Labonne, Dominique Alfandari

Markey Cancer Center Faculty Publications

Monoclonal antibodies are powerful and versatile tools that enable the study of proteins in diverse contexts. They are often utilized to assist with identification of subcellular localization and characterization of the function of target proteins of interest. However, because there can be considerable sequence diversity between orthologous proteins in Xenopus and mammals, antibodies produced against mouse or human proteins often do not recognize Xenopus counterparts. To address this issue, we refined existing mouse monoclonal antibody production protocols to generate antibodies against Xenopus proteins of interest. Here, we describe several approaches for the generation of useful mouse anti-Xenopus antibodies to multiple …

Grassland Productivity And Water Quality: A 21st Century Issue, David M. Nash, P. M. Haygarth

IGC Proceedings (1977-2023)

1. Irrigation and other changes to the hydrological cycle can increase soil and water salinity.
2. Primary salinisation is a natural process that affects much of Europe, Asia, Africa, the Americas and Australia. Secondary salinisation is caused by human activities such as irrigation and land clearing that mobilise salt stored in the soil.
3. The critical water contaminants exported from grasslands are nitrogen, phosphorus, potential pathogens and sediment.
4. The mechanisms responsible for diffuse pollution from grasslands and mitigation strategies are most effectively investigated using a ‘source-mobilisation-transport’ framework.
5. There is a lack of coherent interaction across discipline boundaries that links pollutant sources to impact. …

Water Resources, Agriculture And Pasture: Implications Of Growing Demand And Increasing Scarcity, M. W. Rosegrant, R. A. Valmonte-Santos, S. A. Cline, C. Ringler, W. Li

IGC Proceedings (1977-2023)

1. Water availability for irrigation is threatened in many regions by rapidly increasing demand for nonagricultural water uses in industry, households, and the environment. The scarcity of irrigation water will not only impact crop production, but also meat production, as much of the pasture used to feed livestock is irrigated.
2. Grassland is caught between two countervailing forces: a requirement for increasing meat demand that boosts the need for additional pasture to support livestock production, and rapidly increasing water scarcity that makes pasture irrigation uneconomical.
3. The most effective means of dealing with water scarcity is likely to be conserving water in existing …

Soil Quality Assessment And Management, M. G. Kibblewhite

IGC Proceedings (1977-2023)

1. Soil quality is related to the capacity of soil to deliver ecosystem services on a sustainable basis.
2. Effective management of soil within grasslands can deliver many benefits to mankind but poor management may cause loss of soil quality from erosion, loss of organic matter, physical deterioration etc.
3. Services are delivered from soil by biological processes. Soil quality depends on the form and condition of the soil habitat. Fixed factors (e.g. texture) are useful for assigning soil to types. Variable factors (e.g. organic carbon) can then be used to assess quality within soil types, by reference to percentiles of the distribution …

Grasslands1 For Production And The Environment, David R. Kemp, David L. Michalk

IGC Proceedings (1977-2023)

1. To manage grasslands for production and enhanced environmental values requires a redefinition of the frameworks within which management decisions are made, and a tailoring of practices to suit the ways that farmers operate.
2. Improving the perenniality and permanence of grasslands usually leads to better environmental and production outcomes.
3. There is a case for a more conservative approach to utilising grasslands in order to sustain the functioning of local ecosystems and to improve water quality, nutrient and energy cycling and biodiversity.
4. A landscape rather than paddock focus is more appropriate for meeting current grassland management objectives. Grasslands can be triaged to …

The Potential Of Grassland And Associated Forages To Produce Fibre, Biomass, Energy Or Other Feedstocks For Non-Food And Other Sectors: New Uses For A Global Resource, M. F. Askew

IGC Proceedings (1977-2023)

1. In developed countries increased areas of land will become available for non-food production. Recent reforms of the Common Agricultural Policy will further intensify this trend in Europe.
2. There is potential for grassland and associated species to contribute to large tonnage markets of energy and bulk fibres, to the supply of fermentation products and to speciality markets, but processes and approaches to the market are not as yet developed.
3. There is potential for the establishment of Graminaceous species - specifically for non- food use. For European conditions particular attention is being given to Miscanthus sinensis (Miscanthus), Arundo donax (Giant Reed), Phalaris …

Grasses As Biofactories: Scoping Out The Opportunities, Nick Roberts, K. Richardson, G. Bryan, Christine R. Voisey, W. Mcnabb, T. Conner, M. Christey, R. Johnson

IGC Proceedings (1977-2023)

1. Plant biopharming is set to dominate commercial recombinant protein expression for specific proteins.
2. The choice of plant species depends on a multitude of factors and is determined on a case- by-case basis.
3. As a leaf based expression system grasses would have to compete predominantly with tobacco and alfalfa.
4. The grass-endophyte symbiosis offers a number of unique possibilities for biopharming.

Adoption Of Tropical Legume Technology Around The World: Analysis Of Success, H. M. Shelton, Steven Franzel, M. Peters

IGC Proceedings (1977-2023)

1. Examples of successful adoption of forage legumes are reported from all continents, where they delivered profitability and often provided multipurpose benefits to farmers.
2. Factors vital to successful adoption were: meeting the needs of farmers; building relevant partnerships; understanding the socio-economic context and skills of farmers; participatory involvement with rural communities; and long-term involvement of champions.
3. Organisation of seed supply, achieving scale-up and forming partnerships to implement adoption are key features.
4. Legumes remain an important but under-exploited resource for tropical farming systems. The alternative to legumes will be greater and more costly use of N-fertilisers and purchased protein concentrates.
5. The R&D …

Evolution Of Integrated Crop-Livestock Production Systems, Martin H. Entz, William D. Bellotti, J. M. Powell, S. V. Angadi, W. Chen, K. H. Ominski, B. Boelt

IGC Proceedings (1977-2023)

1. Many factors contribute to changes in the crop-livestock systems, but no logical end-point in the evolution process exists.
2. While benefits of integrated crop-livestock systems over specialised crop and livestock systems are well documented, there has been a move to specialised crop and livestock production.
3. Sustainability issues (manure nutrient concentration, soil quality maintenance, salinity, herbicide resistance, economic instability) have created a renewed interest in integrated crop-livestock systems.
4. Farmer adaptability is as an important link in the evolution between ‘states of integration’.

Interactions Between Foraging Behaviour Of Herbivores And Grassland Resources In The Eastern Eurasian Steppes, Deli Wang, Guodong Han, Yuguang Bai

IGC Proceedings (1977-2023)

1. In rangeland areas such as the eastern Eurasian steppes (Mongolia and China), foraging behaviour is influenced by plant or vegetation properties with high heterogeneity.
2. Until recently foraging theory has not accounted for the foraging process or ingestive behaviour. Existing theories on foraging behaviour need to evolve and begin to coalesce, and combine with observations or manipulative experiments.
3. Plant and patch properties such as diversity and height influence animal foraging behaviour (related to foraging process or diet selection) in heterogeneous steppes.
4. Stocking rate is the most important management factor for grazing or vegetation management, and determining the optimal stocking rate in …