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Southeastern Law Librarian Spring 2023, Seaall

Newsletters

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Effectiveness And Implementation Of Mpath™-Crc: A Mobile Health System For Colorectal Cancer Screening, Anna C. Snavely, Kristie Foley, Ajay Dharod, Mark Dignan, Holly Brower, Elena Wright, David P. Miller Jr.

UK CARES Faculty Publications

Background Screening for colorectal cancer (CRC) is widely recommended but underused, even though CRC is the third most diagnosed cancer and the second leading cause of cancer death in the USA. The mPATH™ program is an iPad-based application designed to identify patients due for CRC screening, educate them on the commonly used screening tests, and help them select their best option, with the goal of increasing CRC screening rates.

Methods The mPATH™ program consists of questions asked of all adult patients at check-in (mPATH™-CheckIn), as well as a module specific for patients due for CRC screening (mPATH™-CRC). In this study, …

Factors Associated With Sun Protection Behaviors Among Childhood Cancer Survivors, Melissa Fluehr, Gary Kwok, Jerod L. Stapleton, Margaret Masterson, Katie A. Devine

UK CARES Faculty Publications

Background: Childhood cancer survivors (CCS) are at increased risk of developing skin cancer. Engaging in sun-protective behaviors may ameliorate that risk, but prior work shows that survivors engage in suboptimal levels of sun-protective behaviors. Guided by the Health Belief Model (HBM), this study evaluated factors associated with sun-protective behavior among CCS.

Methods: This is a secondary analysis of a survey study of 94 adult survivors of childhood cancer recruited from a long-term follow-up clinic. Participants reported their sun protection habits, skin type/ sensitivity, barriers to sun protection, and perceived severity and susceptibility of getting skin cancer. Descriptive statistics were used …

Inhibitory Effect Of Sulfur Dioxide Inhalation On Hering–Breuer Inflation Reflex In Mice: Role Of Voltage-Gated Potassium Channels, Nai-Ju Chan, Chun-Chun Hsu, You Shuei Lin, Ruei-Lung Lin, Lu-Yuan Lee

UK CARES Faculty Publications

Slowly adapting receptors (SARs), vagal mechanosensitive receptors located in the lung, play an important role in regulating the breathing pattern and Hering–Breuer inflation reflex (HBIR). Inhalation of high concentration of sulfur dioxide (SO 2), a common environmental and occupational air pollutant, has been shown to selectively block the SAR activity in rabbits, but the mechanism underlying this inhibitory effect remained a mystery. We carried out this study to determine if inhalation of SO 2 can inhibit the HBIR and change the eupneic breathing pattern, and to investigate further a possible involvement of voltage-gated K þ channels in the inhibitory effect …

Library Satisfaction Survey 2022, Julene L. Jones

Library Presentations

Briefly describes results from the University of Kentucky Libraries' Library Satisfaction Survey which ran Oct.-Nov. 2022. Presented in April 2023 UK Libraries Town Hall

Identification Of A Nacc1-Regulated Gene Signature Implicated In The Features Of Triple-Negative Breast Cancer, Chrispus Ngule, Hami Hemati, Xingcong Ren, Oluwafunminiyi Obaleye, Amos O. Akinyemi, Felix Oyelami, Xiaofang Xiong, Jianxun Song, Xia Liu, Jin-Ming Yang

Markey Cancer Center Faculty Publications

Triple-negative breast cancer (TNBC), characterized by a deficiency in estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor2 (HER2), is among the most lethal subtypes of breast cancer (BC). Nevertheless, the molecular determinants that contribute to its malignant phenotypes such as tumor heterogeneity and therapy resistance, remain elusive. In this study, we sought to identify the stemness-associated genes involved in TNBC progression. Using bioinformatics approaches, we found 55 up- and 9 downregulated genes in TNBC. Out of the 55 upregulated genes, a 5 gene-signature (CDK1, EZH2, CCNB1, CCNA2, and AURKA) involved in cell regeneration was positively correlated …

Leukocyte Tyrosine Kinase (Ltk) Is The Mendelian Determinant Of The Axolotl Melanoid Color Variant, Mirindi Kabangu, Raissa Cecil, Lloyd Strohl Ii, Nataliya Y. Timoshevskaya, Jeramiah James Smith, Stephen Randal Voss

Markey Cancer Center Faculty Publications

The great diversity of color patterns observed among amphibians is largely explained by the differentiation of relatively few pigment cell types during development. Mexican axolotls present a variety of color phenotypes that span the continuum from leucistic to highly melanistic. The melanoid axolotl is a Mendelian variant characterized by large numbers of melanophores, proportionally fewer xanthophores, and no iridophores. Early studies of melanoid were influential in developing the single-origin hypothesis of pigment cell development, wherein it has been proposed that all three pigment cell types derive from a common progenitor cell, with pigment metabolites playing potential roles in directing the …

Incorporating Variability In Kytc Planning And Project Development, Reginald Souleyrette, Teng Wang, Riana Tanzen, Arnold Stromberg

Kentucky Transportation Center Research Report

Transportation planning and design decisions are typically made using point estimates of traffic and safety measures. This report addresses the uncertainty of data used to make these decisions. It focuses on two data elements — project safety ratings and volume estimates from traffic counts. For safety ratings, the Kentucky Transportation Cabinet’s Strategic Highway Investment Formula for Tomorrow (SHIFT) crash history score is used as a case study. Confidence intervals are developed for the Cabinet’s new safety metric, which incorporates estimates of future crashes by severity as well as excess expected crashes above safety goals. For traffic counts, the report develops …

Development And Use Of A Tool For Automated Alignments Of Genes In The Rice Bac's Genbank Card Against Other Species, P. Barre, G. Darrieutort, J. Auzanneau, B. Julier

IGC Proceedings (1977-2023)

In many cases, the analysis of the genetic bases of any trait requires molecular markers and if possible co-dominant PCR-based ones. In perennial fodder species, the number of publicly available markers (microsatellites and Sequence Tagged Site (STS)) is limited. Our goal is to use sequences from model grass species, i.e. rice, wheat, maize, barley, in L. perenne in order to develop STS markers in interesting regions such as under a QTL (Quantitative Trait Loci) or around a candidate gene,. As the genome sequence of rice is now available, the objective was to use the sequences of genes included in the …

Examining The Impact Of Teachers’ Attitudes On College Students’ Attitudes In A Mathematics Classroom, Isiaka Busari, Adeola F. Oladeji, Tolulope O. Sulaimon

Science, Technology, Engineering, and Mathematics (STEM) Education Faculty Publications

This paper discusses the relationship between teachers' attitudes and students' attitudes toward mathematics in the classroom. Through a review of more than twenty relevant pieces of literature, the paper analyzes the impact of teachers' attitudes on students' achievement, performance, confidence, and motivation in mathematics. The study concludes that positive attitudes displayed by teachers can enhance students' positive feelings toward mathematics and increase their engagement in the subject. On the other hand, negative attitudes displayed by teachers can hinder students' motivation and engagement in mathematics. The paper suggests that teacher training programs should prioritize the development of positive attitudes toward mathematics …

Ryegrass Astra: A Web-Based Resource For Lolium Est Analysis, G. C. Spangenberg, T. Sawbridge, E. K. Ong, C. G. Love, T. A. Erwin, E. G. Logan, D. Edwards

IGC Proceedings (1977-2023)

Perennial ryegrass (Lolium perenne L.) is a major grass species of temperate pastoral agriculture.

Genomic Constitution Of Festulolium Varieties, D. Kopecky, V. Cernoch, R. Capka, J. Dolezel

IGC Proceedings (1977-2023)

Hybrids between species of ryegrass (Lolium) and fescue (Festuca) combine useful agronomical characteristics such as rapid establishment from seed and fodder quality from ryegrass and tolerance against abiotic and biotic stressses from fescue. The superior potential of hybrids has stimulated breeding programs generating so called Festulolium varieties. While the varieties have been evaluated extensively for their agronomic characteristics, little information is publicly available on their genomic constitution. The aim of our study was to analyse genomic constitution of a representative set of commercially available European Festulolium cultivars. To do this, we have employed genomic in situ …

Adult Asthma Associated With Roadway Density And Housing In Rural Appalachia: The Mountain Air Project (Map)., W Jay Christian, John Flunker, Beverly May, Susan Westneat, Wayne T Sanderson, Nancy Schoenberg, Steven R Browning

UK CARES Faculty Publications

BACKGROUND: Appalachian Kentucky is a rural area with a high prevalence of asthma among adults. The relative contribution of environmental exposures in the etiology of adult asthma in these populations has been understudied.

OBJECTIVE: This manuscript describes the aims, study design, methods, and characteristics of participants for the Mountain Air Project (MAP), and focuses on associations between small area environmental exposures, including roadways and mining operations, and lifetime and current asthma in adults.

METHODS: A cohort of residents, aged 21 and older, in two Kentucky counties, was enrolled in a community-based, cross-sectional study. Stratified cluster sampling was used to select …

Global Dna Adenine Methylation In Caenorhabditis Elegans After Multigenerational Exposure To Silver Nanoparticles And Silver Nitrate, Anye Wamucho, Jason M. Unrine, John May, Olga V. Tsyusko

UK CARES Faculty Publications

Multigenerational and transgenerational reproductive toxicity in a model nematode Caenorhabditis elegans has been shown previously after exposure to silver nanoparticles (Ag-NPs) and silver ions (AgNO3 ). However, there is a limited understanding on the transfer mechanism of the increased reproductive sensitivity to subsequent generations. This study examines changes in DNA methylation at epigenetic mark N6-methyl-20 -deoxyadenosine (6mdA) after multigenerational exposure of C. elegans to pristine and transformed-via-sulfidation Ag-NPs and AgNO₃ . Levels of 6mdA were measured as 6mdA/dA ratios prior to C. elegans exposure (F₀) after two generations of exposure (F₂) and two generations of …

Clover Astra: A Web-Based Resource For Trifolium Est Analysis, G. C. Spangenberg, T. Sawbridge, E. K. Ong, C. G. Love, T. A. Erwin, E. G. Logan, D. Edwards

IGC Proceedings (1977-2023)

White clover (Trifolium repens L.) is a major temperate forage legume.

Magnetic Nanocomposites For The Remote Activation Of Sulfate Radicals For The Removal Of Rhodamine B, Pranto Paul, Marissa Nicholson, J. Zach Hilt

Chemical and Materials Engineering Faculty Publications

The widespread presence of numerous organic contaminants in water poses a threat to the ecological environment and human health. Magnetic nanocomposites exposed to an alternating magnetic field (AMF) have a unique ability for magnetically mediated energy delivery (MagMED) resulting from the embedded magnetic nanoparticles; this localized energy delivery and associated chemical and thermal effects are a potential method for removing contaminants from water. This work developed a novel magnetic nanocomposite—a polyacrylamide-based hydrogel loaded with iron oxide nanoparticles. For this magnetic nanocomposite, persulfate activation and the contamination removal in water were investigated. Magnetic nanocomposites were exposed to AMF with a model …

Modelling The Encroachment Of Farmhouse Culture On Private Village Pastures And Its Environmental Fall-Out In Northern Western Ghats, India, S. B. Nalavade, K. R. Sahasrabuddhe, A. A. Patwardhan

IGC Proceedings (1977-2023)

Tropical India harbours numerous pasturelands across small landholdings ranging up to few hectares which are covered with grass that is suitable as fodder. These grazing lands are commonly known as ‘Gairan’ in urbanised northern Western Ghats mountain tract in Western India). Such grasslands comprise about 20% of the total area of a village (Jodha, 1986), support livestock and supplement the agro-economy of the village. These pasturelands are being replaced by fenced ‘farmhouses’ of the urban elite, resulting in land use changes that caused drastic qualitative and quantitative changes in terms of area, fodder species composition and livestock they …

Microcystin-Lr Removal From Water Via Enzymatic Linearization And Ultrafiltration, Abelline Fionah, Cannon Hackett, Hazim Aljewari, Laura Brady, Faisal Alqhtani, Isabel C. Escobar, Audie K. Thompson

Chemistry Faculty Publications

Microcystin-LR (MC-LR) is a toxin produced by cyanobacteria that can bloom in freshwater supplies. This study describes a new strategy for remediation of MC-LR that combines linearization of the toxin using microcystinase A, MlrA, enzyme with rejection of linearized byproducts using membrane filtration. The MlrA enzyme was expressed in Escherichia coli (E. coli) and purified via a His-tag with 95% purity. Additionally, composite membranes made of 95% polysulfone and 5% sulfonated polyether ether ketone (SPEEK) were fabricated and used to filter a solution containing cyclic and linearized MC-LR. Tests were also performed to measure the adsorption and desorption of MC-LR …

Integration Of Perennial Ryegrass (L. Perenne) Genetic Maps Using Gene-Associated Snps, A. C. Vecchies, R. C. Ponting, M. C. Drayton, N. O. I. Cogan, K. F. Smith, G. C. Spangenberg, J. W. Forster

IGC Proceedings (1977-2023)

The reference genetic map of perennial ryegrass was developed by the International Lolium Genome Initiative (ILGI), using the p150/112 one-way pseudo-testcross population. A selection of public domain genetic markers including RFLPs, detected by wheat, barley, oat and rice cDNA probes, and AFLPs were mapped, allowing studies of comparative relationships between perennial ryegrass and other Poaceae species. The map was enhanced through the addition of unique perennial ryegrass genomic DNA-derived SSR (LPSSR) markers, providing the basis of framework genetic mapping in other populations. In addition, a small number of RFLP loci detected by candidate genes involved in herbage quality traits were …

Application Of Molecular Markers In Genetic Resources Management Of Perennial Ryegrass, R. Van Treuren

IGC Proceedings (1977-2023)

Key points

1. Molecular markers are effective tools to support traditional approaches in plant genetic resources management.
2. Genetic diversity assessed for perennial ryegrass by AFLP analysis revealed differentiation of populations occurring in traditional Dutch grasslands from commercial varieties, but not from populations occurring in Dutch nature reserves.
3. No specific conservation measures were recommended to maintain the genetic diversity of perennial ryegrass occurring in traditional Dutch grasslands.
4. Pollination rates estimated by microsatellite analysis for a rejuvenated population of a perennial ryegrass genebank accession were very well described by an inverse quadratic function of inter-plant distance between potential mating pairs, while recorded flowering …

New Surficial Geologic Mapping In Kentucky (2021-2022), Matthew Massey, Antonia Bottoms, Max Hammond Iii, Ann Hislop, Meredith Swallom, Michele M. Mchugh

Research Data--KGS

New surficial geologic mapping was performed in nine new 7.5-minute quadrangles in Hardin, Meade, Breckinridge, Grayson, Hart, and Larue Counties, Kentucky.

Quadrangles include Big Clifty, Big Spring (Hardin county only), Custer (Hardin County only), Flaherty, Madrid (Hardin county only), Millerstown, Summit, Tonieville (Hardin county only), and Upton.

Mapping data for each of the nine quadrangles is captured in a ZIP file that contains an ESRI geodatabase and associated FGDC-compliant metadata files (.xml).

The geodatabase is a relational geodatabase of spatial and non-spatial data that conforms to "GeMS (Geologic Map Schema)--a standard format for digital publication of geologic maps", available at …

Heterogeneous Nutrient Distribution Across Dairy Grazing Systems In Southeastern Australia, C. J. P. Gourley, I. Awty, P. A. Durling, J. Collins, A. Melland, S. R. Aarons

IGC Proceedings (1977-2023)

The Australian dairy industry is largely based on a grazed pasture system, although most cows also consume substantial amounts of imported feed (Fulkerson & Doyle 2001). This trend is expected to increase as the Australian dairy industry continues to intensify. Fertiliser inputs of nitrogen (N), phosphorus (P), potassium (K) and sulphur (S) are still viewed as necessary to maintain adequate pasture and milk production despite the fact that most dairy farms are in net positive balance for all of these nutrients (Reuter 2001). Nutrient losses from dairy farming regions and eutrophication of waterways has gained strong public and political attention …

Gene-Associated Single Nucleotide Polymorphism Discovery In White Clover (T. Repens L.), M. C. Drayton, R. C. Ponting, A. C. Vecchies, T. C. Wilkinson, J. George, N. O. I. Cogan, N. R. Bannan, K. F. Smith, G. Spangenberg, J. W. Forster

IGC Proceedings (1977-2023)

Single nucleotide polymorphism (SNP) discovery permits the discovery of molecular marker variation associated with functionally-defined genes. SNP markers have been developed for the temperate pasture legume crop white clover (Trifolium repens) using public and proprietary genic sequences correlated with key agronomic traits of interest.

Translational Genomics For Alfalfa Varietal Improvement, G. D. May

IGC Proceedings (1977-2023)

Key points

1. Medicago truncatula is a model legume with available mapping, genome, and RNA, protein and metabolite profiling databases and genetically diverse populations.
2. Genomics resources developed for M. truncatula have application in the study and improvement of alfalfa making it an excellent model for this forage legume.

Predicting The Effects Of Management On Upland Birds, Economy And Employment, S. M. Gardner, G. M. Buchanan, J. W. Pearce-Higgins, M. C. Grant, A. Waterhouse

IGC Proceedings (1977-2023)

Livestock farming systems play a significant role in the economy and conservation of the UK uplands and rely heavily upon public financial support. Changes in that support could have far-reaching impacts on the wildlife interest and socio-economics of upland areas. Predicting the impacts of such changes is difficult, since they arise from responses to new economic circumstances. The effect of management change is also influenced by natural variation, such as the mosaic of plant communities, already present in the upland landscape. This paper sets out an approach that integrates theoretical models with field studies to investigate the effects of management …

Decision Support For Grassland Systems In Developing Countries, P. K. Thornton

IGC Proceedings (1977-2023)

Key points

1. Information flows in complex systems are often themselves highly complex, and decision support approaches based on linear input-output processes may have only limited impact.

2. How decisions are made, and how they can be appropriately supported, is often incompletely understood, in part because of inadequate understanding of the objectives and attitudes of all the decision makers involved.

3. Much of the developing world faces daunting problems in the coming 30 years, and appropriate information could play a critical role in dealing with these.

4. System complexity, household variability, and institutional intricacies have to be embraced rather than …

Participant Engagement To Develop Report-Back Materials For Personal Air Monitoring, Patrick H. Ryan, Chris Wolfe, Allison Parsons, Cole Brokamp, Ashley Turner, Erin N. Haynes

Epidemiology and Environmental Health Faculty Publications

Background: Studies that measure environmental exposures in biological samples frequently provide participants their results. In contrast, studies using personal air monitors do not typically provide participants their monitoring results. The objective of this study was to engage adolescents who completed personal air sampling and their caregivers to develop understandable and actionable report-back documents containing the results of their personal air sampling.

Methods: Adolescents and their caregivers who previously completed personal air sampling participated in focus groups to guide the development of report-back materials. We conducted thematic analyses of focus group data to guide the design of the report-back …

Measuring Ground Surface Elevation Changes In A Slow-Moving Colluvial Landslide Using Combinations Of Regional Airborne Lidar, Uav Lidar And Uav Photogrammetric Surveys, Sarah E. Johnson, William C. Haneberg, L. Sebastian Bryson, Matthew M. Crawford

UK CARES Faculty Publications

Slow-moving, chronically destructive landslides are projected to grow in number globally in response to precipitation increases from climate change, and land disturbances from wildfire, mining and construction. In the Cincinnati and northern Kentucky metropolitan area, USA, landslides develop in colluvium that covers the steep slopes along the Ohio River and its tributaries. Here we quantify elevation changes in a slow-moving colluvial landslide over 14 years using county- wide lidar, uncrewed aerial vehicle (UAV) structure-from-motion (SfM) surveys and a UAV lidar survey. Because the technology and quality differ between surveys, the challenge was to calculate a threshold of detectable change for …

A Single-Cell Gene Expression Atlas Of Human Follicular Aspirates: Identification Of Leukocyte Subpopulations And Their Paracrine Factors, Yohan Choi, Hayce Jeon, Mats Brännström, James W. Akin, Thomas E. Curry, Misung Jo

UK CARES Faculty Publications

Leukocytes are in situ regulators critical for ovarian function. However, little is known about leukocyte subpopulations and their interaction with follicular cells in ovulatory follicles, especially in humans. Single-cell RNA sequencing (scRNA- seq) was performed using follicular aspirates obtained from four IVF patients and identified 13 cell groups: one granulosa cell group, one thecal cell group, 10 subsets of leukocytes, and one group of RBC/platelet. RNA velocity analyses on five granulosa cell populations predicted developmental dynamics denoting two projections of differentiation states. The cell type-specific transcriptomic profiling analyses revealed the presence of a diverse array of leukocyte-derived factors that can …

Impact Of In Utero Drug Exposure On Neonates Requiring Ecmo: A Retrospective Cohort Study, Hallie Walther, Aric Schadler, Karen Garlitz, John Anthony Bauer, Lindsay Kohler, Erika Waldsmith, Hubert O. Ballard

UK CARES Faculty Publications

The incidence of in utero drug exposure (IUDE) and neonatal extracorporeal membrane oxygenation (ECMO) utilization have both increased over the past decade. However, there are no studies to date that examine the impact that IUDE has on neonates requiring ECMO. In this retrospective cohort study, we compared the clinic course and outcomes of neonates who were placed on ECMO with IUDE vs. neonates without IUDE. Analysis included data extracted from medical records from all neonatal ECMO runs between January 2014 and January 2021 at the University of Kentucky Children’s Hospital. A total of 56 neonatal patients were placed on ECMO …