Digital Commons Network^™

Neuroinflammation And Neurodegeneration In Adult Rat Brain From Binge Ethanol Exposure: Abrogation By Docosahexaenoic Acid, Nuzhath Tajuddin, Kwan-Hoon Moon, Simon Alex Marshall, Kimberly Nixon, Edward J. Neafsey, Hee-Yong Kim, Michael A. Collins

Pharmaceutical Sciences Faculty Publications

Evidence that brain edema and aquaporin-4 (AQP4) water channels have roles in experimental binge ethanol-induced neurodegeneration has stimulated interest in swelling/edema-linked neuroinflammatory pathways leading to oxidative stress. We report here that neurotoxic binge ethanol exposure produces comparable significant effects in vivo and in vitro on adult rat brain levels of AQP4 as well as neuroinflammation-linked enzymes: key phospholipase A2 (PLA2) family members and poly (ADP-ribose) polymerase-1 (PARP-1). In adult male rats, repetitive ethanol intoxication (3 gavages/d for 4 d, ∼ 9 g/kg/d, achieving blood ethanol levels ∼ 375 mg/dl; "Majchrowicz" model) significantly increased AQP4, Ca+2-dependent PLA2 GIVA (cPLA2), phospho-cPLA2 GIVA …

Healthcare For Persons With Intellectual And Developmental Disability In The Community, David A. Ervin, Brian Hennen, Joav Merrick, Mohammed Morad

Pediatrics Faculty Publications

INTRODUCTION: While there has been impressive progress in creating and improving community healthcare delivery systems that support people with intellectual and developmental disabilities (IDD), there is much more that can and should be done.

METHODS: This paper offers a review of healthcare delivery concepts on which new models are being developed, while also establishing an historical context. We review the need for creating fully integrated models of healthcare, and at the same time offer practical considerations that range from specific healthcare delivery system components to the need to expand our approach to training healthcare providers. The models and delivery systems, …

Democratizing The Food System- The Food Policy Council Movement, Garrett Spear

Kaleidoscope

The increasing complexity of our modern food system has changed the landscape of American agriculture and diet, and has increasingly consolidated decision-making abilities in a concentrated group of organizations. Local food movements are re-appropriating their food supply chains through a variety of methods, including the democratic-minded food policy council organization. Food policy councils organize community stakeholders into a network of organizations which can collectively grapple with food issues affecting and on behalf of the local community. This study consisted of research on the history and methods of the food policy council movement, directed ultimately at the council-less context in Lexington …

Biomarkers Of Acute Myocardial Infarction In The Elderly: Troponin And Beyond, Martin G. Rains, Charles A. Laney, Alison L. Bailey, Charles L. Campbell

Gill Heart & Vascular Institute Faculty Publications

In the broadest context, biological markers, or biomarkers, are molecules that characterize a biological system or process. In the setting of cardiovascular disease, a number of biomarkers have become an integral part of diagnostic and risk stratification strategies. In this review, we will discuss classic and emerging biomarkers of cardiovascular disease and the role of these biomarkers in the diagnosis and prognosis of elderly patients presenting with acute myocardial infarction.

Nutritional Requirement For Protein Production From Trehalose Synthesis In The Male Accessory Gland Of Tribolium Castaneum, Ashlee Anciro

Kaleidoscope

No abstract provided.

Redox Proteomic Identification Of Hne-Bound Mitochondrial Proteins In Cardiac Tissues Reveals A Systemic Effect On Energy Metabolism After Doxorubicin Treatment, Y. Zhao, Sumitra Miriyala, L. Miao, Mihail I. Mitov, David M. Schnell, Sanjit Kumar Dhar, J. Cai, J. B. Klein, Rukhsana Sultana, D. Allan Butterfield, Mary Vore, I. Batinic-Haberle, Subbarao Bondada, Daret K. St. Clair

Toxicology and Cancer Biology Faculty Publications

Doxorubicin (DOX), one of the most effective anticancer drugs, is known to generate progressive cardiac damage, which is due, in part, to DOX-induced reactive oxygen species (ROS). The elevated ROS often induce oxidative protein modifications that result in alteration of protein functions. This study demonstrates that the level of proteins adducted by 4-hydroxy-2-nonenal (HNE), a lipid peroxidation product, is significantly increased in mouse heart mitochondria after DOX treatment. A redox proteomics method involving two-dimensional electrophoresis followed by mass spectrometry and investigation of protein databases identified several HNE-modified mitochondrial proteins, which were verified by HNE-specific immunoprecipitation in cardiac mitochondria from the …

A Filtration System That Greatly Reduces Aluminum In Calcium Gluconate Injection, Usp Used To Prepare Parenteral Nutrition Solutions, Robert A. Yokel, Wesley R. Harris, Christopher D. Spilling, Vasiliy P. Abramov, Jason M. Lone, Robert J. Kuhn

Pharmaceutical Sciences Faculty Publications

OBJECTIVE: The study objective was to reduce aluminum (Al) in Calcium Gluconate Injection, US Pharmacopeia (USP) used in the preparation of parenteral nutrition (PN) solutions.

METHODS: A flow-through filter containing an immobilized chelator that complexes Al from Calcium Gluconate Injection, USP as it flows through the filter was designed, refined by design modifications, and extensively tested. When a small-volume parenteral vial containing 100 mL of Calcium Gluconate Injection, USP is connected on the inlet side of the filter, and the outlet side is connected to an evacuated receiving vial, the filtered solution is drawn into the receiving vial. This constitutes …

Tissue Triage And Freezing For Models Of Skeletal Muscle Disease, Hui Meng, Paul M. L. Janssen, Robert W. Grange, Lin Yang, Alan H. Beggs, Lindsay C. Swanson, Stacy A. Cossette, Alison Frase, Martin K. Childers, Henk Granzier, Emanuela Gussoni, Michael W. Lawlor

Biostatistics Faculty Publications

Skeletal muscle is a unique tissue because of its structure and function, which requires specific protocols for tissue collection to obtain optimal results from functional, cellular, molecular, and pathological evaluations. Due to the subtlety of some pathological abnormalities seen in congenital muscle disorders and the potential for fixation to interfere with the recognition of these features, pathological evaluation of frozen muscle is preferable to fixed muscle when evaluating skeletal muscle for congenital muscle disease. Additionally, the potential to produce severe freezing artifacts in muscle requires specific precautions when freezing skeletal muscle for histological examination that are not commonly used when …

Income, Program Participation, Poverty, And Financial Vulnerability: Research And Data Needs, James P. Ziliak

University of Kentucky Center for Poverty Research Discussion Paper Series

The aim of this paper is to assess the adequacy of the data infrastructure in the United States to meet future research and policy evaluation needs as it pertains to income, program participation, poverty, and financial vulnerability. I first discuss some major research themes that are likely to dominate policy and scientific discussions in the coming decade. This list includes research on the long-term consequences of income inequality and mobility, issues of transfer-program participation and intergenerational dependence, challenges with poverty measurement and poverty persistence, and material deprivation. I then summarize what information we currently collect in the U.S. that is …

Biomechanical Properties Of Hemlocks: A Novel Approach To Evaluating Physical Barriers Of The Plant-Insect Interface And Resistance To A Phloem-Feeding Herbivore, Paul Ayayee, Fuqian Yang, Lynne K. Rieske

Entomology Faculty Publications

Micromechanical properties that help mediate herbivore access may be particularly important when considering herbivorous insects that feed with piercing-sucking stylets. We used microindentation to quantify the micromechanical properties of hemlock, Tsuga spp., to quantify the hardness of the feeding site of the invasive hemlock woolly adelgid, Adelges tsugae. We measured hardness of the hemlock leaf cushion, the stylet insertion point of the adelgid, across four seasons in a 1 y period for four hemlock species growing in a common garden, including eastern, western, mountain, and northern Japanese hemlocks. Leaf cushion hardness was highest in the fall and winter and …

Forage News [2014-06], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• KFGC Field Day
• KFGC Award Nominations
• Beef Bash - Hold the Date
• U.K. Forage Workers Tour
• Kentucky Grazing School
• Impact of Forage Species (Quality) on Stocker Average Daily Gains
• Southern Forages: An Information Transfer "Success Story"
• Native Grass Perceptions: Producer and Professionals Surveys in Tennessee
• Economics of Native Grass Forage Production: How We Got Lucky and Where to from Here?
• Tolerance of Four Clover Species to Varying Rates of Pre and Post-Emergent Broadleaf Pasture Herbicides

Resveratrol And Cancer: Focus On In Vivo Evidence, Lindsay G. Carter, John A. D'Orazio, Kevin J. Pearson

Graduate Center for Nutritional Sciences Faculty Publications

Resveratrol is a naturally occurring polyphenol that provides a number of anti-aging health benefits including improved metabolism, cardioprotection, and cancer prevention. Much of the work on resveratrol and cancer comes from in vitro studies looking at resveratrol actions on cancer cells and pathways. There are, however, comparatively fewer studies that have investigated resveratrol treatment and cancer outcomes in vivo, perhaps limited by its poor bioavailability when taken orally. Although research in cell culture has shown promising and positive effects of resveratrol, evidence from rodents and humans is inconsistent. This review highlights the in vivo effects of resveratrol treatment on breast, …

Practice-Based Learning: Opportunities And Implications For Stem Education, Glen P. Mays

Health Management and Policy Presentations

This presentation examines how practice-based research strategies have been used to produce new knowledge and stimulate learning about how to improve the organization, financing, and delivery of public health services. I consider how practice-based research strategies can be adapted to advance knowledge and practice related to science, technology, engineering and mathematics (STEM) education and application.

Innovative Tertiary Prevention For Women Who Seek Shelter Services Greenhouse 17, Diane Fleet, Diane R. Follingstad, Claire M. Renzetti

NSF-NIJ IPV Prevention Workshop

No abstract provided.

Producing Population Health: Collective Action Requires Infrastructure, Incentives & Evidence, Glen P. Mays

Health Management and Policy Presentations

Population health improvement strategies are collective action problems that require targeted infrastructure, incentives, and information to succeed. Research on collective action problems and solutions in public health and other spheres of practice offer insight for the successful scale and spread of population health innovations.

Sirt1 Deletion Leads To Enhanced Inflammation And Aggravates Endotoxin-Induced Acute Kidney Injury, Rong Gao, Jiao Chen, Yuxin Hu, Zhenyu Li, Shuxia Wang, Sreerama Shetty, Jian Fu

Toxicology and Cancer Biology Faculty Publications

Bacterial endotoxin has been known to induce excessive inflammatory responses and acute kidney injury. In the present study, we used a mouse model of endotoxemia to investigate the role of Sirt1 in inflammatory kidney injury. We examined molecular and cellular responses in inducible Sirt1 knockout (Sirt1-/-) mice and wild type littermates (Sirt1+/+) in lipopolysaccharide (LPS)-induced kidney injury. Our studies demonstrated that Sirt1 deletion caused aggravated kidney injury, which was associated with increased inflammatory responses including elevated pro-inflammatory cytokine production, and increased ICAM-1 and VCAM-1 expression. Inflammatory signaling such as STAT3/ERK phosphorylation and NF-κB activation was markedly elevated in kidney tissues …

Effects Of Maternal Depression On Family Food Insecurity, Kelly Noonan, Hope Corman, Nancy E. Reichman

University of Kentucky Center for Poverty Research Discussion Paper Series

Theory suggests that adverse life events—such as unemployment or health shocks—can result in food insecurity, which has increased substantially in the U.S. over the past decade alongside the obesity epidemic. We test this proposition by estimating the effects of a specific and salient mental health event—maternal depression during the postpartum year—on child and family food insecurity. Using data from the Early Childhood Longitudinal Study—Birth Cohort, we estimate the effects of maternal depression on food insecurity using both single- and twostage models, and explore potential buffering effects of relevant public assistance programs and supports. We find that moderate to severe maternal …

Forage News [2014-05], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Kentucky Grazing School
• Milestone Forage Program Dates
• Hay Quality
• Mountain Ag Week at UK Robinson Center
• Forage Recovery from the Winter of 2013/14
• Hay Harvest Stage Impacts Quality
• KFGC Field Day

Lowering Somatic Cell Count With Best Management Practices, Stephen F. Higgins, Kylie Schmidt, Sarah Wightman

Agriculture and Natural Resources Publications

As health and food safety concerns grow, dairy producers are facing more stringent regulations. In 2010, the European Union (EU) set the somatic cell count (SCC) upper limit, an indicator of milk quality, for exported milk at 400,000 cells per milliliter. However, the current U.S. SCC limit is 750,000 cells per milliliter. As of January 2012, any U.S. milk used in export markets must meet the EU standards. It is projected that US milk processors will gradually adopt the EU upper limit, making it difficult for dairy producers to sell milk containing more than 400,000 somatic cells per milliliter. Dairy …

An Ipm Scouting Guide For Common Problems Of Apple In Kentucky, Nicole Ward Gauthier, Ricardo Bessin, John Strang, Shawn Wright, Cheryl A. Kaiser

Agriculture and Natural Resources Publications

The National Integrated Pest Management Network defines IPM as “a sustainable approach to managing pests by combining biological, cultural, physical and chemical tools in a way that minimizes economic, health, and environmental risks.” One of the key components of IPM is to continually scout and monitor crops to identify problems before they result in significant economic losses. Proper identification of pathogens and insect pests as well as nutritional and physiologic disorders and even herbicide drift is essential to determining the proper course of action. The pictures included in this guide represent some common pests or problems that growers may encounter …

Editorial Comment: A Method For Identifying Positive Deviant Local Health Departments In Maternal And Child Health, Sharla A. Smith, Michael A. Preston, Marylou Wallace

Frontiers in Public Health Services and Systems Research

Improving the well-being of mothers, infants, and children is a vital public health initiative in the United States. Local health departments are designated agents for addressing maternal and child health outcomes. Klaiman and colleagues used a positive deviant (PD) framework in identifying the modifiable activities and approaches for LHDs that contribute to better MCH outcomes.

A Method For Identifying Positive Deviant Local Health Departments In Maternal And Child Health, Tamar A. Klaiman, Athena Pantazis, Betty Bekemeier

Frontiers in Public Health Services and Systems Research

Local health departments (LHDs) are under increasing pressure to improve performance with limited resources. While research has found that financial resources may be associated with better health outcomes, there are some LHDs that maintain exceptional performance, even with limited budgets. Our interest was identifying LHDs that positively deviate in MCH outcomes compared to their peers while taking into account local context including geography and finances. We found that our method for identifying positive deviants was effective, and that LHD expenditures may not be linked to MCH outcomes. The next step in our work is to conduct in-depth analysis with positive …

Health Reform Implementation: Update And Implications For Kentucky, Glen P. Mays

Health Management and Policy Presentations

This talk reviews the progress of health reform implementation in Kentucky with a focus on opportunities for aligning systems for medical care, public health, and social support delivery to improve population health.

Unifying Systems For Population Health: Infrastructure, Incentives & Evidence For Collective Action, Glen P. Mays

Health Management and Policy Presentations

This presentation, part of the SBM Presidential Symposium on Aligning Family, Clinical, and Community Systems, reviews the collective action problems that are commonly encountered in implementing multi-sector population health improvement strategies, and examines research on ways of using public health strategies and infrastructure to overcome these problems.

Trends In The Use, Sociodemographic Correlates, And Undertreatment Of Prescription Medications For Chronic Obstructive Pulmonary Disease Among Adults With Chronic Obstructive Pulmonary Disease In The United States From 1999 To 2010, Earl S. Ford, David M. Mannino, Anne G. Wheaton, Letitia Presley-Cantrell, Yong Liu, Wayne H. Giles, Janet B. Croft

Preventive Medicine and Environmental Health Faculty Publications

BACKGROUND: The extent to which patients with COPD are receiving indicated treatment with medications to improve lung function and recent trends in the use of these medications is not well documented in the United States. The objective of this study was to examine trends in prescription medications for COPD among adults in the United States from 1999 to 2010.

METHODS: We performed a trend analysis using data from up to 1426 participants aged ≥20 years with self-reported COPD from six national surveys (National Health and Nutrition Examination Survey 1999-2010).

RESULTS: During 2009-2010, the age-adjusted percentage of participants who used any …

Harnessing Community Engaged Scholarship For Collective Action To Improve Population Health, Glen P. Mays

Health Management and Policy Presentations

The growing body of theoretical and empirical scholarship on collective action problems offers important lessons about strategies for improving health on a population-wide basis.

From Adair To Woodford County And All Points In-Between, Jason Keinsley, Lauren Robinson

Library Presentations

At 
the
 Agricultural 
Information 
Center 
(AIC) 
we 
are 
trying 
to 
provide
 relevant
 content
 for
 Cooperative
 Extension
 personnel
 located
 throughout 
the 
state 
of 
Kentucky.
 
The
 College
 of 
Agriculture, 
Food, and 
Environment
 is 
the 
University 
of 
Kentucky's
 largest
 college 
and
 has
 employees
 located
 in 
all 
of 
the 
120 
Kentucky
 counties.
 
Therefore,
 we 
are 
working 
to 
create
 and
 maintain
 relevant 
outreach
 services
 for
 a 
geographically 
and
 culturally
 diverse 
user 
group.
 
Throughout 
this
 process,
 we
 have
 realized
 that
 creating
 online
 tutorials
 and
 virtual
 training
 sessions
 is
 not
 enough
 to
 support
 outreach
 services—we must
 stay 
visible. 

To 
stay 
visible, 
we
 have
 visited 
regional
 meetings
 and …

Creating Learning Systems: Lessons From Public Health Practice-Based Research Networks, Glen P. Mays

Health Management and Policy Presentations

Practice-based research networks (PBRNs) provide powerful mechanisms for implementing research studies that evaluate the health and economic effects of delivery system innovations. This presentation reviews strategies, emerging findings, and lessons learned from PBRNs and related studies conducted in U.S. public health settings. We give special focus to studies that examine multi-organizational and cross-sectoral strategies for population health improvement.

Beyond Income: What Else Predicts Very Low Food Security Among Children?, Patricia M. Anderson, Kristin F. Butcher, Hilary W. Hoynes, Diane Whitmore Schanzenbach

University of Kentucky Center for Poverty Research Discussion Paper Series

We examine characteristics and correlates of households in the United States that are most likely to have children at risk of inadequate nutrition – those that report very low food security (VLFS) among their children. Using 11 years of the Current Population Survey, plus data from the National Health and Nutrition Examination Survey and American Time Use Survey, we describe these households in great detail with the goal of trying to understand how these households differ from households without such severe food insecurity. While household income certainly plays an important role in determining VLFS among children, we find that even …

Global Kentucky (Spring 2014), University Of Kentucky International Center

Global Kentucky

No abstract provided.