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Up Regulation Of Heat Shock Protein 70b (Hsp70b) And Ssa1 In Chlamydomonas Reinhardtii Via Hsp70a-Rbcs2 And Psad Promoter, B. Kirtley Amos

Theses and Dissertations--Biosystems and Agricultural Engineering

Fabrication of effective algae cultivation systems adjacent to coal-fired power plants to fixate waste CO₂ would represent a sizable step towards achieving a carbon neutral energy cycle. However, emission gas would elevate the algal cultivation system temperature and decreases its pH without expensive preprocessing. Increased temperature and acidity constitutes a profound stress on the algae. Although stressed algae produce heat shock proteins (HSPs) that promote protein folding and protect against stress, the ordinary biological response is insufficient to protect against coal flue gas. Experimental upregulation of HSPs could make algae respond to the stress caused by high temperatures and …

Dietary Selenium Supplementation: Effects On Neurodegeneration Following Traumatic Brain And Spinal Cord Injury, Carolyn A. Crowdus Meyer

Theses and Dissertations--Neuroscience

Traumatic brain and spinal cord injury continue to be substantial clinical problems with few available treatment strategies. Individuals who are at a greater risk for sustaining a central nervous system (CNS) injury, such as professional athletes and military personnel, may benefit from a prophylactic supplement that would intervene in the neurodegenerative pathways immediately following injury. The high demand for selenium within the central nervous system, as well as the synthesis of selenoproteins by neurons and astrocytes suggests a critical role of selenium within the brain and spinal cord. Studies were designed to test the efficacy of enriched dietary selenium status …

Regulation Of Pancreatic Β-Cell Function By The Renin-Angiotensin System In Type 2 Diabetes, Robin C. Shoemaker

Theses and Dissertations--Pharmacology and Nutritional Sciences

Diet-induced obesity promotes type 2 diabetes (T2D). Drugs that inhibit the renin-angiotensin system (RAS) have been demonstrated in clinical trials to decrease the onset of T2D. Previously, we demonstrated that mice made obese from chronic consumption of a high-fat (HF) diet have marked elevations in systemic concentrations of angiotensin II (AngII). Pancreatic islets have been reported to possess components of the renin-angiotensin system (RAS), including angiotensin type 1a receptors (AT1aR), the primary receptor for AngII, and angiotensin converting-enzyme 2 (ACE2), which negatively regulates the RAS by catabolizing AngII to angiotensin-(1-7) (Ang-(1-7)). These two opposing proteins have been implicated in the …

Zhx2 Regulation Of Lipid Metabolism And The Balance Between Cardiovascular And Hepatic Health, Kate Townsend Creasy

Theses and Dissertations--Pharmacology and Nutritional Sciences

The growing obesity epidemic in America carries with it numerous health risks, including diabetes, increased serum lipid levels, and excess fat accumulation in the liver. If these conditions persist or become exacerbated, they may lead to the development of cardiovascular disease, the current leading cause of death among Americans, or to nonalcoholic fatty liver disease (NAFLD) which can progress to hepatocellular carcinoma (HCC), one of the deadliest forms of cancer. Better understanding of the genes involved in these diseases can lead to improved identification of at-risk individuals and treatment strategies.

Our lab previously identified zinc fingers and homeoboxes 2 (Zhx2) …

Competition’S Effect On Teacher Pay And Teacher Characteristics, Casey S. Brasher

Theses and Dissertations--Economics

This dissertation adds to the literature that examines the effect of competition and school choice reform in elementary and secondary education. Specifically, we explore how three major forms of competition in U.S. schooling markets in Tiebout competition, charter competition, and private school competition affect the teaching profession by estimating a teacher wage equation and series of teacher characteristic equations. Since it has been well-established that all 3 forms of competition are likely endogenous, we present 2SLS estimates of competition’s effect in addition to simple OLS estimates. Our findings confirm the importance of studies controlling for endogeneity concerns, particularly in regards …

Eating Spain: National Cuisine Since 1900, Matthew J. Wild

Theses and Dissertations--Hispanic Studies

Analyzing cookbooks, gastronomic guides, literature and film, this dissertationoutlines the creation of a Spanish national cuisine. Studying the works of Carmen de Burgos, Emilia Pardo Bazán, Dionisio Pérez, Ana María Herrera, Juan Mari Arzak and Ferrán Adrià among others, the project examines the evolution of this nationalist discourse by identifying common and recurring themes in an effort to extrapolate and describe the historical and cultural evolution of food from 1900 to the present day.

Within the framework of Food and Cultural Studies, this project treats cookbooks, culinary manifestos and guidebooks as texts. Influenced by a variety of culinary and gastronomic …

Evidence-Based Hospitals, David R. Bardach

Theses and Dissertations--Epidemiology and Biostatistics

In 2011 the University of Kentucky opened the first two inpatient floors of its new hospital. With an estimated cost of over $872 million, the new facility represents a major investment in the future of healthcare in Kentucky. This facility is outfitted with many features that were not present in the old hospital, with the expectation that they would improve the quality and efficiency of patient care. After one year of occupancy, hospital administration questioned the effectiveness of some features. Through focus groups of key stakeholders, surveys of frontline staff, and direct observational data, this dissertation evaluates the effectiveness of …

Developments In Nonparametric Regression Methods With Application To Raman Spectroscopy Analysis, Jing Guo

Theses and Dissertations--Epidemiology and Biostatistics

Raman spectroscopy has been successfully employed in the classification of breast pathologies involving basis spectra for chemical constituents of breast tissue and resulted in high sensitivity (94%) and specificity (96%) (Haka et al, 2005). Motivated by recent developments in nonparametric regression, in this work, we adapt stacking, boosting, and dynamic ensemble learning into a nonparametric regression framework with application to Raman spectroscopy analysis for breast cancer diagnosis. In Chapter 2, we apply compound estimation (Charnigo and Srinivasan, 2011) in Raman spectra analysis to classify normal, benign, and malignant breast tissue. We explore both the spectra profiles and their derivatives to …

Novel Role Of Intestinal Lipid Transport In Food Allergy And Cholesterol Homeostasis, Jianing Li

Theses and Dissertations--Pharmacology and Nutritional Sciences

The small intestine is the main organ for food digestion and nutrient absorption. It is constantly exposed to antigen and immunomodulatory agents from diet and commensal microbiota. Thus, the intestine is the largest compartment of the immune system in the body. Peanuts and many other allergen resources contain triglycerides, which may affect the antigen absorption through the intestine, but their effects on sensitization and anaphylaxis are unknown. We found that medium chain triglycerides (MCT) promoted antigen absorption into Peyer’s Patches, rather than into the blood directly. Both gavage and feeding of MCT plus peanut protein induced spontaneous allergic sensitization. MCT-sensitized …

The Role Of Apob-Containing Lipoproteins In Abdominal Aortic Aneurysm, Jing Liu

Theses and Dissertations--Pharmacology and Nutritional Sciences

Abdominal aortic aneurysm (AAA) is a devastating disease that exhibits permanent lumen expansion typically in the infrarenal aorta. AAA is prevalent among aged population, especially in males. Despite the incidence in women is lower, studies indicate the tortuosity is more severe and aortic rupture risk is higher in women. In most patients, AAA remains asymptomatic until it ruptures leading to sudden and fatal hemorrhage.

To date, there is no proven medical therapy that can prevent the expansion or rupture. Human observational studies implicate the presence of AAA is associated with both high plasma low-density lipoprotein-cholesterol (HDL-C) and low plasma high-density …

Tumor-Derived Proteins And Mitochondrial Dysfunction In Lung Cancer-Induced Cachexia, Julie B. Mclean

Theses and Dissertations--Physiology

Lung tumors secrete multiple factors that contribute to cachexia, a severe wasting syndrome that includes loss of muscle mass, weakness, and fatigue. 80% of advanced lung cancer patients experience cachexia, which cannot be reversed by nutritional interventions, diminishes response to and tolerance of cancer treatments, and increases morbidity and mortality. Despite a multitude of clinical trials, there are currently no approved treatments. This deficiency suggests that not all of the factors that contribute to cachexia have been identified.

Cancer is frequently accompanied by an increase in cyclooxygenase-2 (COX-2), a hallmark of inflammation. Clinical trials for COX-2 inhibitors have resulted in …

The Skeletal Muscle Molecular Clock Regulates The Timing Of Substrate Metabolism And The Circadian Expression Of Titin-Cap, Brian A. Hodge

Theses and Dissertations--Physiology

Skeletal muscle is a major contributor to whole-body metabolism as it serves as a depot for both glucose and amino acids, and is a highly metabolically active tissue. An intrinsic molecular clock mechanism exists within skeletal muscle that regulates the timing of physiological processes. A key function of the clock is to regulate the timing of metabolic processes to anticipate time of day changes in environmental conditions. The purpose of this study was to identify metabolic genes that are expressed in a circadian manner and determine if these genes are regulated downstream of the intrinsic molecular clock by assaying gene …

Psychological Well-Being Among Breast Cancer Survivors: Factors That Influence Transition From Primary Treatment To Early Survivorship, Celestine G. Gochett

Theses and Dissertations--Nursing

Eudaimonic psychological well-being (PWB) refers to a lifelong process of purposeful engagement in goal-driven tasks or activities resulting in positive psychological functioning. These activities reflect autonomy, purpose in life, self-acceptance, personal growth, positive relations with others, and environmental mastery. While more women are surviving breast cancer, they face a multitude of late and long term physiopsychosocial challenges that result from being diagnosed and treated. Protective health effects associated with the experience of PWB can potentially mitigate ill-being and benefit overall health of breast cancer survivors (BCS). Adequate preparation by healthcare teams to transition them from primary treatment to early survivorship …

The Impact Of Prenatal Depressive Symptoms, Intimate Partner Relationship, And Immune Status On Postpartum Depression, Julia J. Hall

Theses and Dissertations--Nursing

The prevalence of depression during pregnancy in the U. S. is approximately 13%. Poor quality of the intimate partner relationship is significantly correlated with depression during pregnancy. The adverse effects of antenatal depression have been widely documented. The relationship between the intimate partner relationship and depression during pregnancy has not been well delineated in the literature. Little data exist regarding the impact of prenatal immune status on risk for postpartum depression. Due to limited evidence, there is a critical need to examine the relationship among trimester specific cytokines, quality of the intimate partner relationship, and antenatal depressive symptoms on risk …

An Investigation Into The Occurrence Of Batrachochytrium Dendrobatidis Infection In Plethodontid Salamander Communities Of Robinson Forest, Sarah H. Spaulding

Theses and Dissertations--Forestry and Natural Resources

Environmental and anthropogenic stressors negatively affect amphibians in a variety of ways, often increasing their vulnerability to pathogen infection and mortality. Sampling for the pathogenic fungus Batrachochytrium dendrobatidis (Bd) was conducted in order to: 1) determine the presence of chytrid infection in stream-associated plethodontid salamanders of southeastern Kentucky, and 2) evaluate differences in infection intensity between salamanders residing in intact forest streams, timber-harvested streams, and surface-mined streams. During 14 sampling sessions occurring between March, April and May of 2013, DNA samples from 306 individual salamanders within 8 species from the family Plethodontidae were collected; additional amphibians (i.e. frogs, newts) were …

Invasiveness Of An Exotic Gall Wasp Is Influenced By Interactions With Exotic And Endemic Organisms, Ignazio Graziosi

Theses and Dissertations--Entomology

American chestnut was once a crucial component of North American forests, but it was functionally eliminated by the introduction of the chestnut blight fungus. Chestnut is recently experiencing resurgence, but the introduced Asian chestnut gall wasp, a specialist herbivore, threatens chestnut recovery. I characterized this invasion and the interactions developing with host associates as the gall wasp spread in North America. Gall wasp dispersal is attributable to host plant distribution, effects of prevailing winds during adult insect emergence, and to topography. This knowledge may be useful to more accurately predict locations of future gall wasp infestations. Gall wasp invasiveness is …

Sex, Dementia, And Long-Term Care: Public Perspectives, Erin L. Yelland

Theses and Dissertations--Family Sciences

The current mixed methods study utilized an ecological framework to examine public perspectives toward sexual behaviors among long-term care residents with dementia. Analyzing attitudes of the public is an integral component of understanding the entire ecological system that affects the development and overall well-being of a long-term care resident. Attitudes were examined using a multiple segment factorial vignette with a probability sample of 329 respondents from a southern state. Results indicate that attitudes were not statistically affected by sex or the elapsed time since diagnosis, and age, spousal disposition, and degree of intimacy predicted attitudes. A respondent’s education level also …

Food Security Factors Affect Growth In Young Children In An Ecuadorian Indigenous Community, Whitney Jeannine Asher

Theses and Dissertations--Nutrition and Food Systems

Inadequate nutrition in preschool-aged children in an urban indigenous community outside of Santo Domingo, Ecuador has a negative impact on growth and development. Nutritional assessments have shown that children are underweight and that there are some effects of stunting and wasting in the population. This study was conducted to assess the extent of food security in this indigenous community in preparation for addressing two of the Millennium Development Goals for 2015: reducing the under-five child mortality rate by two-thirds and eradicating extreme poverty and hunger. An aim was to validate on-site assessment measures in this indigenous community regarding geographic circumstances, …

New Insights Into The Function And Development Of The Soldier Caste In Termites, Li Tian

Theses and Dissertations--Entomology

The evolution of nonreproductive castes is a defining characteristic of eusociality. The function and developmental regulation of the altruistic worker and soldier caste is the central element contributing to major advantages of eusociality over solitary animals. The soldier caste is the first evolved sterile caste in termites. Their primary function is believed to be colony defense. However, the function and development of termite soldiers remains largely unknown. Because of their apparent morphological adaptation for fighting and their limited behavior repertoire, our understanding of colony defense by termite soldiers is limited to their physical defense. In addition, we know little about …

Dietary Habits And Knowledge Of College Age Students, Matthew R. Majors

Theses and Dissertations--Nutrition and Food Systems

College is a time of emerging identity and habit formation for students. One hundred and sixty-two (162) college students were surveyed via an internet based survey. The survey asked questions regarding dietary habits such as consumption of dairy, vegetables, fruit, and protein. The survey also examined dietary knowledge such as ability to read food labels. The students were divided into two groups for analysis, nutrition majors and non-nutrition majors. The two groups differed in Body Mass Index (BMI) (p=0.004), but not nutrition knowledge (p=0.082). The nutrition students had an average BMI of 22.0+/-2.42 and a nutrition knowledge score of 2.44+/-0.60, …

The Role And Impact Of Cooperative Extension In Diabetes Self-Management Education, Brittany Michelle Murray

Theses and Dissertations--Nutrition and Food Systems

Diabetes is increasing globally and nationally. Diabetes complications and costs can be reduced through modification of lifestyle risks and diabetes self-management education (DSME). The Cooperative Extension System (CES) is uniquely positioned to implement DSME. This study assessed the role and impact of the Cooperative Extension System (CES) in DSME. A survey was sent to CES professionals throughout the U.S. a total of 43 participants provided information on 73 DSME programs. Most participants were from the South (n=22, 51.16%) and Midwest (n=12, 27.91%) and most programs targeted adults with and at risk for type 2 diabetes. Most programs were developed and …

Impact Of The Healthy Hunger-Free Kids Act On Changes In The Phytochemical Content Of School Lunch Menus And Implications Of Science-Based Nutrition Education On Promoting Student Identification Of Foods High In Phytochemicals, Siddhi Lalit Shroff

Theses and Dissertations--Nutrition and Food Systems

Concern that youth do not have enough fruit and vegetable intake lead to two strategies implemented to influence intake in the school environment: the Healthy Hunger-Free Kids Act (HHFKA) and Fighting with Food: Battling Chemical Toxicity with Good Nutrition program (FF), which could influence phytochemical content and knowledge regarding phytochemicals, respectively. Individual food logs (n=468) were assessed upon completion of FF curriculum to determine whether students were able to correctly apply their nutrition knowledge regarding FF. Menus from one district were analyzed pre-HHFKA and post-HHFKA in elementary (n=156), middle (n=171), and high schools (n=171), for change in the frequency of …

Student Perceptions Of Organic Food In Relation To Health, Environment And Pricing, Stacey Paterson

Theses and Dissertations--Nutrition and Food Systems

The organic industry is full of opinions and perceptions, which influence consumer purchasing decisions. A convenience sample of 69 college students between the ages of 19-23 registered in a basic food preparation class participated in this study. A sensory analysis of organic and conventional fruits, vegetables, meat and poultry, eggs and dairy measuring categorical taste differences between the two types of food. Student perceptions of organic foods in relation to health, environment and pricing and the likelihood of purchase were also studied. The influence of Diet and Well-being on organic purchases predominantly mattered somewhat to students, while price mattered completely. …

The Relationships Among Bmi, Waist Circumference, Weight Loss And Health Indicators, John R. Rupp

Theses and Dissertations--Nutrition and Food Systems

Current health recommendations encourage weight loss for overweight and obese individuals. However, the importance of weight to health is still unclear. The purpose of this study was to determine the impact of weight loss on overall health in a sample of adults who have completed a 10 or 12 week weight loss intervention. The study sample included males and females (n =99) between the ages of 27 -64 years that had a BMI of 29-45 kg/m². The study design is a secondary data analysis of data from two different behavioral weight loss interventions. Both interventions prescribed a reduced calorie diet, …

The Role Of Kentucky State-Supported Postsecondary Education In Creating A Healthier Citizenship, Julia K. Buchanan

Theses and Dissertations--Educational Leadership Studies

Citizens within the United States of America (USA) and the Commonwealth of Kentucky exhibit indicators of lessened health status in a variety of areas. Many chronic diseases and conditions are due to individual lifestyle behaviors, which can be modified through the implementation of dedicated health and wellness programming. Such programs, often housed within institutions of higher education, have the ability to impact many individuals including students, faculty, staff, and community members. This dissertation is a report of a mixed-methods study that begins to explore how state-supported postsecondary institutions may be able to impact individual behavior and thus, resulting health outcomes. …

Front Matter [Preface And List Of Contributors], National Organizing Committee, International Grassland Congress

IGC Proceedings (1977-2023)

No abstract provided.

Promising Forage Options To Enhance Livestock Production In Mediterranean Climate Agricultural Systems, Serkan Ates, Hayley C. Norman, Hichem Ben Salem, Bradley Nutt, Harun Cicek

IGC Proceedings (1977-2023)

The increasing demand for food, fibre and animal products inevitably requires intensifying agricultural production worldwide. This can present a number of environmental and farming systems challenges in Mediterranean climate areas of the world. Developments of novel strategies, employing ecologically sound intensification practices, are crucial to enhance production in the Mediterranean agropastoral production systems. In this paper, we have discussed forage based systems that improve productivity, profitability, environmental quality and resilience of the farming systems. Key opportunities include better use and integration of diverse genetic resources, new agronomic techniques and the utilization of perennial forage plants that are adapted to land …

Grazing Management Options For Maintaining Optimum Pasture Composition And Utilization, Glen E. Aiken

IGC Proceedings (1977-2023)

Global demand for meat and dairy products will continue to increase over the 21st century, however, the global forage-based livestock industry will be challenged in meeting production goals with minimal impact of the environment. Sustainable production of grazing livestock will depend on the use of carefully planned grazing management strategies. Development of a grazing management plan involves making two decisions: determining the livestock density at which pastures are stocked and settling on the method used to graze the pastures. Stocking rate indirectly affects output per animal and per hectare through its direct effect on forage mass and pasture composition. A …

Management Of Pasture Soils: Biochar Stability, Carbon Storage Potential And Its Effect On Production And Quality, Bhupinder Pal Singh, Yunying Fang, Mark Boersma, Damian Collins, Lukas Van Zwieten, Lynne M. Macdonald

IGC Proceedings (1977-2023)

The use of biochar has been proposed as a stable carbon (C) amendment with long-term carbon (C) storage potential in agricultural soils while improving primary productivity. However, this concept has not been widely tested in contrasting soils under temperate pasture systems. To address this knowledge gap, a ¹³C-labelled biochar, produced from Eucalyptus saligna biomass by slow pyrolysis (450° C; d¹³C -36.7‰) was surface (0"10 cm) applied in C₃ dominated, annual temperate pasture systems across Arenosol, Cambisol and Ferralsol. The results show that only 2% of the applied biochar-C was mineralised in a relatively clay- and C-poor …

Grassland Renovation And Consequences For Nutrient Management, Johannes Isselstein, Manfred Kayser

IGC Proceedings (1977-2023)

Sward degradation is a serious threat to the functioning of grassland and the provision of ecosystem services. Renovation measures are frequently applied in order to restore degraded swards. However, the success is highly variable and substantial tradeoffs are often found following renovation such as among agronomic and environmental services. Starting from a general classification of renovation measures the paper investigates the processes induced by renovation that lead to a change of the vegetation and that affect carbon and nitrogen fluxes. These processes are strongly interrelated and dependent on site, climate and management condition as well as on the time scale. …