Digital Commons Network^™

C-Abl And Arg Drive Cancer Chemoresistance Via Activation Of Multiple Signaling Pathways, Jonathan Thomas Sims

Theses and Dissertations--Pharmacology and Nutritional Sciences

Despite 35 years of clinical trials, there has been little improvement in one-year survival rates with any chemotherapeutic regimen for the treatment of metastatic melanoma due to resistance to all known agents. Regardless of advances in detection and prevention, diagnosis of metastatic disease remains a death sentence. Resistance mechanisms, including aberrant kinase signaling and drug transport pumps, indicate a need for identification of other therapeutic targets that impinge upon multiple signaling pathways. The Abl family of non-receptor tyrosine kinases (c-Abl, Arg) has been indicted as a causative force in leukemia for more than three decades; however, their role in solid …

The Cardiac L-Type Calcium Channel Distal Carboxyl- Terminus Auto-Inhibition Is Regulated By Calcium, Shawn M. Crump

Theses and Dissertations--Physiology

The L-type calcium channel (LTCC) provides trigger Ca²⁺ for sarcoplasmic reticulum Ca²⁺-release and LTCC function is influenced by interacting proteins including the LTCC Distal Carboxyl-terminus (DCT) and calmodulin. DCT is proteolytically cleaved, and re-associates with the LTCC complex to regulate calcium channel function. DCT reduces LTCC barium current (IBa,L) in reconstituted channel complexes, yet the contribution of DCT to ICa,L in cardiomyocyte systems is unexplored. This study tests the hypothesis that DCT attenuates cardiomyocyte ICa,L. We measured LTCC current and Ca²⁺ transients with DCT co-expressed in murine cardiomyocytes. We also heterologously co-expressed DCT and CaV1.2 constructs …

Fiscal Federalism And Spatial Interactions Among Governments, Longjin Chen

Theses and Dissertations--Economics

This dissertation examines multiple state and local expenditure categories in the United States to expand understanding of fiscal federalism and spatial interactions among governments. First, the author investigates the relationship between police expenditures and crime rates from a spatial perspective. Both police expenditures and crime rates in one state are found to exhibit a similar pattern to that in neighboring states. Spatial correlation is also detected between police expenditures and crime rates. As police of neighbors in fact deter crime at home, there are positive externalities present among the states. Second, the author conducts new tests on the Leviathan hypothesis, …

Three Essays Concerning The Relationship Between Exports, Macroeconomic Policy, And Economic Growth, Brandon James Sheridan

Theses and Dissertations--Economics

This dissertation consists of three essays that collectively investigate the relationship between exports, macroeconomic policy and economic growth. The first essay investigates the relationship between disaggregated exports and growthto address why many developing countries rely on primary goods as their main source of export income when evidence suggests they could earn higher returns by exporting manufactured goods.Using regression tree analysis, I find that although increasing manufacturing exports is important for sustained economic growth, this relationship only holds once a threshold level of development is reached. The results imply that a country needs a minimum level of education before it is …

The Risk Of Violence And Intimate Partner Choice Within A Risk Society, Adam J. Pritchard

Theses and Dissertations--Sociology

This dissertation examines the influence of competing risks in shaping individuals' choices about potential intimate relationships. According to Ulrich Beck's "risk society" theory, the individualization of social risks has direct and measurable consequences for the ways people organize and evaluate potential intimate relationships (Beck & Beck-Gernsheim, 1995, 2002, 2004; Giddens, 1994; Lupton, 2006). This study investigates the ways in which subjective or identity-related risks hypothesized by scholars of late modernity shape the perception and the actual risk of dating violence. Empirical research on dating violence identifies many objective “risk factors” related to a person’s chances of experiencing intimate partner violence; …

Temperature And Strain Controlled Optimization Of Stabilization Of Polyacrylonitrile Precursor Fibers, Mark Parr Taylor

Theses and Dissertations--Mechanical and Aerospace Engineering

Carbon fiber is one of the leading materials for high strength and modulus, and light weight applications. Improvements in carbon fiber properties are directly dependent on all aspects of manufacture, especially the process of stabilization. Therefore, it is the goal of this thesis to study the effects of the temperature and strain profile of the stabilization process, and the resulting carbon fiber tensile properties. In addition, the precursor fibers used were spun under two different draw ratios, to study the effects of the spinning parameters. Results indicated through DMA studies that completeness of stabilization reactions can be gauged by the …

Transport Phenomena Associated With Liquid Metal Flow Over Topographically Modified Surfaces, Wen Liu

Theses and Dissertations--Mechanical and Aerospace Engineering

Brazing and soldering, as advanced manufacturing processes, are of significant importance to industrial applications. It is widely accepted that joining by brazing or soldering is possible if a liquid metal wets the solids to be joined. Wetting, hence spreading and capillary action of liquid metal (often called filler) is of significant importance. Good wetting is required to distribute liquid metal over/between the substrate materials for a successful bonding.

Topographically altered surfaces have been used to exploit novel wetting phenomena and associated capillary actions, such as imbibitions (a penetration of a liquid front over/through a rough, patterned surface). Modification of surface …

From Celluloid Realities To Binary Dreamscapes: Cinema And Perceptual Experience In The Age Of Digital Immersion, Edwin Lloyd Mcguy Lohmeyer

Theses and Dissertations--Art and Visual Studies

Technologies in digital cinema are quickly changing the way contemporary filmmakers create films and how audiences currently perceive them. As we move onward into the digital turn, it becomes ever more apparent that the medium of film has been emancipated from its dependence on the photograph. Directors are no longer required to capture the objectively real as it sits before the photographic lens, but can essentially construct it via groundbreaking advancements in computer-generated imagery, motion capture technology, and digital 3D camera systems and display technologies. Since the origins of film, spectators and filmmakers have assumed an existing relationship between reality …

Applying Specific Arts Activities To Improve The Quality Of Life For Individuals With Alzheimer’S Disease And Dementia, Ann Christianson Tietyen

Theses and Dissertations--Art and Visual Studies

This study examined the effectiveness of a combination of seven different visual art activities, hat decoration, collage, embossing, painting, ceramics, photography, and printmaking, on quality of life for eight veterans with Alzheimer’s disease and dementia. The eight veterans were selected from the population of residents at the Thomson‐ Hood Veterans facility in Wilmore, Kentucky. These veterans were administered the seven art activities mentioned above, which ranged from less difficult to increasing difficulty. Three standard self‐reporting instruments, the Quality of Life‐AD, the Rosenberg Self‐Esteem Scale, and the Smiley‐Face Mood Assessment, as well as systematic observation and surveys were used to explore …

Discovery Of Gz-793a, A Novel Vmat2 Inhibitor And Potential Pharmacotherapy For Methamphetamine Abuse, David B. Horton

Theses and Dissertations--Pharmacy

Methamphetamine abuse is a serious public health concern affecting millions of people worldwide, and there are currently no viable pharmacotherapies to treat methamphetamine abuse. Methamphetamine increases extracellular dopamine (DA) concentrations through an interaction with the DA transporter (DAT) and the vesicular monoamine transporter-2 (VMAT2), leading to reward and abuse. While numerous studies have focused on DAT as a target for the discovery of pharmacotherapies to treat psychostimulant abuse, these efforts have been met with limited success. Taking into account the fact that methamphetamine interacts with VMAT2 to increase DA extracellular concentrations; the focus of the current work was to develop …

Molecular Mechanisms Of Thromboxane A2 Receptor-Mediated Invasion In Lung Cancer Cells, Xiuling Li

Theses and Dissertations--Pharmacy

Thromboxane A2 receptor (TP) has been shown to play important roles in multiple aspects of cancer development including regulation of tumor growth, survival and metastasis. Molecular mechanisms of TP mediated cancer cell invasion remain to be identified. TP agonist, I-BOP, significantly elevated several matrix metalloproteinases (MMPs) including MMP-1, MMP-3, MMP-9 and MMP-10 in A549 human lung adenocarcinoma cells overexpressing TPα (A549-TPα) or TPβ (A549-TPβ). Signaling pathways of I-BOP-induced MMP-1 expression were examined in further detail as a model system for MMPs induction. Signaling molecules involved in I-BOP-induced MMP-1 expression were identified by using specific inhibitors including small interfering (si)-RNAs of …

Localization And Functional Characterization Of Oatp4c1 Transporter In In Vitro Cell Systems And Human/Rat Tissues, Kuei-Ling Kuo

Theses and Dissertations--Pharmacy

The organic anion transporting polypeptide 4c1 (Oatp4c1) was previously identified as a novel uptake transporter predominantly expressed at the basolateral membrane in the rat kidney proximal tubules. Its functional role was suggested to be a vectorial transport partner of an apically-expressed efflux transporter for the efficient translocation of physiological substrates into urine, some of which were suggested to be uremic toxins. In vitro studies in polarized cell lines showed that upon transfection rat Oatp4c1 localizes at the apical membrane. The objectives of this project were to further validate the subcellular localization of Oatp4c1/OATP4C1 in rat and human tissues as well …

Nanocrystals Of Chemotherapeutic Agents For Cancer Theranostics: Development And In Vitro And In Vivo Evaluation, Christin P. Hollis

Theses and Dissertations--Pharmacy

The majority of pharmacologically active chemotherapeutics are poorly water soluble. Solubilization enhancement by the utilization of organic solvents often leads to adverse side effects. Nanoparticle-based cancer therapy, which is passively targeted to the tumor tissue via the enhanced permeation and retention effect, has been vastly developed in recent years. Nanocrystals, which exist as crystalline and carry nearly 100% drug loading, has been explored for delivering antineoplastic agents. Additionally, the hybrid nanocrystal concept offers a novel and simple way to integrate imaging agents into the drug crystals, enabling the achievement of theranostics. The overall objective of this dissertation is to formulate …

Role Of Cyclooxygenase-2 In Abdominal Aortic Aneurysms In Mice, Kamalika Mukherjee

Theses and Dissertations--Pharmacy

Abdominal aortic aneurysm (AAA) is a chronic inflammatory disease with no available pharmacological treatment. AAA formation reduces the structural integrity of the vessel and increases the susceptibility to rupture. The inflammatory response within human aneurysmal tissue is characterized by increased expression of cyclooxygenase-2 (COX-2). Similarly, in a mouse model of the disease induced by chronic Angiotensin II (AngII) infusion, we have shown that COX-2 expression in the abdominal aortic smooth muscle layer increases early in the development of the disease. Furthermore, genetic or pharmacological inactivation of COX-2 prior to disease initiation reduces AAA incidence.

The current study utilized nonhyperlipidemic mice …

The Effect Of Personal Scandal On Celebrity Athletes And Shopper’S Purchase Intentions And Attitude Favorability, Mary A. Graham

Theses and Dissertations--Retailing and Tourism Management

Athletes have become more than sports professionals; they are million dollar investments for brand images. Businesses worldwide have transitioned old promotional schemes to athlete endorsements and have experienced positive reactions to the public change. Athletes connected with consumers on a heroic level and translated the brand’s message to purchasers through the theory of transference of affect (White, Goddard, & Wilbur, 2009). Subsequently, there had been an equal rise in the caution businesses exercised as several athletes found occupancy in negative press. Those involved in scandals posed reputational risks for businesses and could reduce positive transference to consumers. The purpose of …

The Influence Of Culture: A Comparison Of White American And Hispanic American Teens Information Sources And Store Attribute Preferences, Kelli H. Murphy

Theses and Dissertations--Retailing and Tourism Management

The culture of America is changing now more than ever. With the Hispanic American population growing, it is important for retailers to understand how to market to these changing demographics. This study compares white Americans to Hispanic Americans in regard to store selection influences. Information sources and store attributes are analyzed as factors that influence store selection. It was found that acculturation of Hispanic American teens has created many commonalities between the two cultures. Future research could become be more in depth as to why these things occur or their effect on actual purchases.

Rhodococcus Equi Infection And Interferon-Gamma Regulation In Foals, Lingshuang Sun

Theses and Dissertations--Veterinary Science

Rhodococcus equi (R. equi) is one of the most serious causes of pneumonia in young foals. The clinical disease is of great concern to breeding farms worldwide due to the impact of mortality on economic losses. While adult horses are resistant to R. equi, foals exhibit a distinct age-associated susceptibility. The mechanism underlying this susceptibility in foals is not well understood. Interferon-gamma (IFNg) plays an important role in the clearance of R. equi, but its expression is impaired in neonatal foals. Moreover, the regulation of this age-related IFNg expression in foals remains unknown. In humans, IFNg …

Effects On Semen Quality And On Establishment Of Persistent Equine Arteritis Virus (Eav) Infection In Stallions Following Experimental Challenge With The Kentucky 84 (Ky84) Strain, Juliana Roberta Campos

Theses and Dissertations--Veterinary Science

Equine arteritis virus (EAV) is the causal agent of equine viral arteritis (EVA), a disease of equids. Following EAV infection, up to 70% of stallions may become carriers and continuously shed the virus in their semen for varying time periods. The long-term carrier stallion has an important role in the transmission and maintenance of EAV in horse populations. Recently, it has been demonstrated a correlation between in vitro susceptibility of CD3⁺ T lymphocytes to EAV infection and establishment of long-term persistent infection among stallions following natural infections. In this study, we investigated whether stallions with in vitro EAV susceptible …

Control And Passive Treatment Of Runoff From Horse Muck Storage Structures Using Rain Gardens, Hillary K. Otte

Theses and Dissertations--Forestry and Natural Resources

Runoff from livestock operations may contain a variety of pathogens and high levels of nutrients and other harmful contaminants, and is of particular concern in central Kentucky as watersheds are threatened by waste generated from a high concentration of equine activity. Rain gardens are a type of stormwater management tool used to capture and passively treat runoff. This project aimed to incorporate rain gardens into the horse muck storage structures at a thoroughbred facility in the Canr Run watershed in Lexington, Kentucky. Water quality data from soil water within two rain garden muck pads and two control pads, and grab …

Glycerol-3-Phosphate Is A Novel Regulator Of Basal And Induced Defense Signaling In Plants, Bidisha Chanda

Theses and Dissertations--Plant Pathology

Plants use several strategies to defend themselves against microbial pathogens. These include basal resistance, which is induced in response to pathogen encoded effector proteins, and resistance (R) protein-mediated resistance that is activated upon direct or indirect recognition of pathogen encoded avirulence protein(s). The activation of Rmediated signaling is often associated with generation of a signal, which, upon its translocation to the distal uninfected parts, confers broad-spectrum immunity against related or unrelated pathogens. This phenomenon known as systemic acquired resistance (SAR) is one of the well-established forms of induced defense response. However, the molecular mechanism underlying SAR remains largely unknown. Induction …

An Analysis Of The Influence Of Nutrition Information On Consumers’ Restaurant Menu Choices, Joseph Adam Higgins

Theses and Dissertations--Nutrition and Food Systems

Obesity has risen drastically in the past few decades. Some experts contribute this to an increased prevalence of eating food away from home and consuming large portion sizes. A popular discussion in policy and legislation arenas has been mandatory implementation of nutrition information on restaurant chain menus. Menu labeling in restaurants that have 20 or more locations nationally was first implemented by New York City in an effort to increase consumer awareness of the calorie content of menu entrees. Since New York City’s implementation of restaurant menu labeling in 2008, there have been conflicting studies on whether nutrition menu labeling …

Perception And Usage Of Video Streaming/Electronic Media In Nutrition Education For Kentucky Individuals, Beth Ann Oldiges

Theses and Dissertations--Nutrition and Food Systems

Nutrition Education Professionals (NEP) working for Extension Agents of Kentucky were surveyed to evaluate their use of a variety of tools in nutrition education in three areas: nutrition, food safety, and food preparation. The purpose of this research was to determine the perception and usage of video streaming/electronic media among community nutrition professionals as a means to better educate individuals in Kentucky and furthermore, to determine if demographics of NEP affect their utilization of this technology. The results concluded that regardless of demographics, NEP perceive video streaming/electronic media to be effective in nutrition education. However, age, employment length, and video …

Nutrition Knowledge Of Consumers And Health Professionals And The Use Of Menu Calorie Labeling, Kate Louise Perkins

Theses and Dissertations--Nutrition and Food Systems

The purpose of this study is to assess the need for menu labeling of calories based on the ability of food consumers to identify lower calorie options. By surveying consumers in Kentucky and Ohio and health professionals in Kentucky about their awareness of caloric content, basic nutrition knowledge and ability to choose lower calorie options, we can predict the potential benefit of menu labeling initiatives and the amount of education that will be necessary for consumers to effectively use menu labeling to make informed decisions on calorie intake. Through online surveys, it was determined that consumers were better able to …

Cross-Cultural Comparison Of Perceptions About School Feeding Programs From Ghanaian And U.S. Adults, Yolanda L. Jackson

Theses and Dissertations--Nutrition and Food Systems

Because of an increase in food insecurity over the past decade, the U.S. and many developing countries are focusing efforts on issues relating to child hunger. School feeding programs have been in existence for roughly 220 years but modifications have been made over the years to improve the quality of food they serve. Little research has been conducted about adult perceptions of school feeding programs and the impact it has on the community in the U.S. as well as developing countries such as Ghana, West Africa. Because of the gap in literature on school feeding programs and adult perceptions about …

Characteristics Of Kentucky Agricultural Operations Participating In Nrcs Conservation Programs, Carmen T. Agouridis

MPA/MPP/MPFM Capstone Projects

The Natural Resource Conservation Service (NRCS) designs and promotes a wide variety of conservation practices and programs that enhance the environment by reducing soil erosion, improving water quality, and enhancing and creating wildlife habitat. The impact of these practices and programs is largely dependent on the voluntary participation of landowners. Thus, central to the success of the NRCS conservation programs is an understanding of the characteristics of landowners and operations participating in these programs.

Using operator and operation characteristics from the 1997, 2002, and 2007 Censuses of Agriculture and controlling for county fixed effects, this study 1) identifies significant characteristics …

Do College Football Games Affect The Level Of Crime In The Local Community?, Micah Johnson

MPA/MPP/MPFM Capstone Projects

On the local news channels in major college football towns, there are anecdotal stories during that detail celebratory riots that took place during or after a college football game. Few empirical studies have focused on whether there is a relationship between college football games and crime. This paper attempts to determine that relationship by exploiting the fact that college football games are played in a home stadium and an away stadium.

More specifically, the study addressed the following two questions:

Do jurisdictions in which a home football game is played differ in crime rate from those where an away game …

Can Drug Court Results Be Predicted? Completion And Termination Of Drug Court Participants, Christopher D. Geisler

MPA/MPP/MPFM Capstone Projects

The substantial growth in Kentucky’s prison population since the 1980s has resulted in increased spending on corrections, both the total amount and as a percentage of total general fund spending. The sustained growth in corrections spending combined with shrinking budgetary realities has created an incentive to explore ways to reduce costs without compromising public safety. Community supervision programs are one alternative to addressing this problem. They are an attractive approach to dealing with certain types of criminals because the cost is significantly lower than incarceration. Drug courts are an example of community supervision that allows drug offenders to avoid imprisonment …

A Changing Perspective On The Role Of Neuroinflammation In Alzheimer's Disease, Donna M. Wilcock

Physiology Faculty Publications

Alzheimer's disease (AD) is a complex, neurodegenerative disorder characterized by the presence of amyloid plaques and neurofibrillary tangles in the brain. Glial cells, particularly microglial cells, react to the presence of the amyloid plaques and neurofibrillary tangles producing an inflammatory response. While once considered immunologically privileged due to the blood-brain barrier, it is now understood that the glial cells of the brain are capable of complex inflammatory responses. This paper will discuss the published literature regarding the diverse roles of neuroinflammation in the modulation of AD pathologies. These data will then be related to the well-characterized macrophage phenotypes. The conclusion …

Evaluation Of Algae Concentration In Manure Based Media, Maira Freire Pecegueiro Do Amaral

Theses and Dissertations--Biosystems and Agricultural Engineering

Algae can be used to treat wastewater and manure while producing a feedstock for renewable energy. Algae require nutrients to achieve their maximum growth and manure could provide those nutrients, thereby reducing the cost of algae production and the impact of manure treatment. Algae concentration during cultivation is a critical variable that is difficult to measure due to the high concentration of suspended solids present in manure. This dissertation addresses methods to measure algae concentration in the presence of manure solids.

Quantifying the algae concentration gravimetrically or by optical density was unreliable due to manure solids interfering with the measurement. …

Rhetorics Of Empire: The Falangist Discourse Of War (1939-1943), M. Elena Aldea Agudo

Theses and Dissertations--Hispanic Studies

During the Spanish Civil War (1936-1939) a mix of right-wing ideologies existed among the Francoist forces. In sharp contrast with the Republican forces, the Francoist insurgents were successful in banding together despite their ideological differences. However, in the postwar era, this relative unity gave way to a struggle among the different ideological positions, each striving to impose its agenda for the new State. The party Falange Española Tradicionalista y de las Juntas de Ofensiva Nacional Sindicalista (FET y de las JONS) assumed power, but was not entirely successful in advancing its totalitarian project, which it had inherited from the prewar …