Digital Commons Network^™

Sterol Lipid Metabolism In Down Syndrome Revisited: Down Syndrome Is Associated With A Selective Reduction In Serum Brassicasterol Levels, Gavin Tansley, Daniel T. Holmes, Dieter Lütjohann, Elizabeth Head, Cheryl L. Wellington

Pharmacology and Nutritional Sciences Faculty Publications

Over the past 15 years, insights into sterol metabolism have improved our understanding of the relationship between lipids and common conditions such as atherosclerosis and Alzheimer's Disease (AD). A better understanding of sterol lipid metabolism in individuals with Down Syndrome (DS) may help elucidate how this population's unique metabolic characteristics influence their risks for atherosclerosis and AD. To revisit the question of whether sterol lipid parameters may be altered in DS subjects, we performed a pilot study to assess traditional serum sterol lipids and lipoproteins, as well as markers of sterol biosynthesis, metabolites, and plant sterols in 20 subjects with …

Suicide In Prepubertal Children, Kimberly K. Mcclanahan, Hatim A. Omar

Pediatrics Faculty Publications

No abstract provided.

Youth And Student Suicide, Kimberly K. Mcclanahan, Hatim A. Omar

Pediatrics Faculty Publications

No abstract provided.

Suicidal Behavior And Prevention In Adolescence, Hatim A. Omar, Joav Merrick

Pediatrics Faculty Publications

Suicidal behavior in youth is not uncommon and currently one of the leading causes of death in adolescence in many countries around the world. Individual risk factors for suicidality in youth include: psychiatric disorder; certain personality characteristics; genetics; gender; sexual orientation; and previous suicide attempts. Family psychopathology and environmental factors such as media contagion also contribute as risk factors. Developmental issues, including: the establishment of independence and intimate relationships; as well as the pursuit of personal and career goals; may also provide stressors leading to suicidality. Prevention and intervention strategies are considered and include: early detection and treatment of mental …

Sexually Transmitted Diseases In Adolescence, Donald E. Greydanus, Jane Seyler, Hatim A. Omar, Colleen B. Dodich

Pediatrics Faculty Publications

High rates of unprotected sexual behavior in adolescents result in millions of sexually transmitted diseases (STDs) in the world. This paper reviews factors inducing high STD rates, specific STDs, and their management based on 2010 US Centers for Disease and Prevention (CDC) STD guidelines. Clinicians should screen all their sexually active adolescent patients for STDs and provide preventive education as well as treatment measures.

Novel Mechanism Leading To Mismatch Repair Deficiency And Mutator Phenotype, Janice Ortega Rodríguez

Theses and Dissertations--Toxicology and Cancer Biology

DNA mismatch repair (MMR) is a critical genome-maintenance system. It ensures genome stability by correcting mismatches generated during DNA replication, suppressing homologous recombination, and inducing apoptosis in response to severe DNA damage. As a result, defects in MMR lead to genome-wide mutations and susceptibility to both hereditary and sporadic cancer syndromes. The hallmark of cancer cells defective in MMR is their ability to display frequent instability in simple repetitive DNA sequences, a phenomenon called microsatellite instability (MSI). However, only ~70% of the MSI-positive tumors have identifiable MMR gene mutations, indicating that additional factor(s) are responsible for the MSI phenotype in …

Physiological And Toxicological Roles Of Abc Transporters In Cellular Efflux Of Substrates, Donna J. Coy

Theses and Dissertations--Toxicology and Cancer Biology

ATP-binding cassette (ABC) transporters are transmembrane proteins that transport a wide variety of substrates across intra and extra-cellular membranes. A few examples of endo and xenobiotic substrates are metabolic products, lipids, sterols, and drugs. An important function of ABC transporters involved in export is to prevent intracellular the buildup of toxic products. Several ABC transporters have also been associated with drug resistance upon treatment with chemotherapeutic agents. P-glycoprotein (P-GP) and the multidrug resistant (MRP) transporters of the ABC C family are examples of transporters that confer chemo-resistance.

We have studied two unique roles of ABC transporters in the liver and …

The Influence Of Selenium Status On Immune Function And Antioxidant Status In The Horse, Mieke Brummer

Theses and Dissertations--Animal and Food Sciences

Selenium (Se) has received a lot of attention for its antioxidant and immune modulating properties. Yet, comparably few studies have focused on the horse. Therefore the objectives of this research were to evaluate the influences of Se status on immune function and antioxidant defense in horses. Twenty eight horses were allocated to one of 4 dietary Se treatments: low (LS), adequate (AS), high organic (SP) and high inorganic (SS). First, horses assigned to LS, SP and SS were depleted of Se and received a low Se diet (0.07 ppm Se) for 35 wk, while AS received an adequate Se diet …

A Library Is Not The Books: An Ethical Obstacle To The Digital Library, James M. Donovan

Law Faculty Scholarly Articles

Casual and thoughtful speakers alike frequently use “library” as though it were the collective noun for “book”: A herd of cows, a murder of crows, a library of books. In practice it matters little whether “book” is understood as a specific physical artifact of ink and paper, or whether it refers more generically to any information-containing entity. The consistent point appears to be that in the presence of a sufficient number of those items, a library necessarily rises into existence.

This implied relationship proves critical to debates over the implications of digital formats for libraries. If libraries are reducible to …

Post-Reform Medicaid Before The Court: Discordant Advocacy Reflects Conflicting Attitudes, Nicole Huberfeld

Law Faculty Scholarly Articles

The United States Supreme Court heard two Medicaid cases this term that raise major questions about the program and the tensions it creates between the federal and state governments. On October 3, 2011, the Court heard oral arguments in Douglas v. Independent Living Center of Southern California, a dispute between California and its Medicaid providers regarding reimbursement cuts resulting from California's budget crisis. The Medicaid providers argued that the proposed cuts are so extreme as to violate federal law and thus the Supremacy Clause of the United States Constitution. Their contention hinged on the Equal Access Provision of the Medicaid …

Validation Of Computational Fluid Dynamic Simulations Of Membrane Artificial Lungs With X-Ray Imaging, Cameron Christopher Jones

Theses and Dissertations--Biomedical Engineering

The functional performance of membrane oxygenators is directly related to the perfusion dynamics of blood flow through the fiber bundle. Non-uniform flow and design characteristics can limit gas exchange efficiency and influence susceptibility of thrombus development in the fiber membrane. Computational fluid dynamics (CFD) is a powerful tool for predicting properties of the flow field based on prescribed geometrical domains and boundary conditions. Validation of numerical results in membrane oxygenators has been predominantly based on experimental pressure measurements with little emphasis placed on confirmation of the velocity fields due to opacity of the fiber membrane and limitations of optical velocimetric …

Performing Community: The Place Of Music, Race And Gender In Producing Appalachian Space, Deborah J. Thompson

Theses and Dissertations--Geography

Traditional, participatory music is a powerful medium through which people express and shape their ideas about identity, mobility, social relations, and belonging, and through which people are in turn shaped. The everyday cultural practices of playing, sharing, and dancing to traditional music, as well as discussions about the nature of traditional music and production of events involving traditional music, all work to construct the region called Appalachia.

Through this dissertation, I seek to answer some simple questions that have complicated answers involving place, identity, power, and social relations, with economic, social, and emotional ramifications: Who gets to be an Appalachian …

Use Of Hybrid Diffuse Optical Spectroscopies In Continuous Monitoring Of Blood Flow, Blood Oxygenation, And Oxygen Consumption Rate In Exercising Skeletal Muscle, Katelyn Gurley

Theses and Dissertations--Biomedical Engineering

This study combines noninvasive hybrid diffuse optical spectroscopies [near-infrared spectroscopy (NIRS) and diffuse correlation spectroscopy (DCS)] with occlusive calibration for continuous measurement of absolute blood flow (BF), tissue blood oxygenation (S_tO₂), and oxygen consumption rate (VO₂) in exercising skeletal muscle. Subjects performed rhythmic dynamic handgrip exercise, while an optical probe connected to a hybrid NIRS/DCS flow-oximeter directly monitored oxy-, deoxy-, and total hemoglobin concentrations ([HbO₂], [Hb], and [tHb]), S_tO₂, relative BF (rBF), and relative VO₂ (rVO₂) in the forearm flexor muscles. Absolute baseline BF and …

Identifying Hot-Spots Of Fecal Contamination In The Royal Spring Karstshed, Samuel C. Lee

Theses and Dissertations--Civil Engineering

The City of Georgetown, Kentucky relies on a vast karst spring network as a drinking water source. This karst feature has several inputs from sinkholes and streams in the Cane Run Watershed: a watershed associated with a variety of land uses in the recharge area. The recharge area encompasses the area from North Lexington to Georgetown and is composed of urban, suburban, agricultural and industrial usage. A serious water quality issue exists with respect to the impact of fecal contamination within the spring recharge area. Identification of fecal contamination is quantified by microbial indicators adapted from surface water applications: fecal …

Los Fantasmas Queer De La Dictadura Franquista: ¡Toda Una Re-Velación!, Danae Gallo González

Theses and Dissertations--Modern and Classical Languages, Literature and Cultures

This paper is part of the academic effort to recover historical memory in post-Civil War Spain and metaphorically applies the so-called Giobert Tincture to Carmen Martín Gaite’s El cuarto de atrás (1978), Dulce Chacón’s La voz dormida (2002) and Pedro Almodóvar’s La mala educación (2004) in order show how these works reveal the ghosts of the repression exerted against the epitome of the abject/obscene by Franco’s dictatorship: the queer collective. This collective continues to suffer from marginalization as well as from the effects of repression. I argue that El cuarto de atrás reveals C.’s repressed hybrid/queer identity and sexual orientation, …

Contested Spaces In London: Exhibitionary Representations Of India, C. 1886-1951, Alayna Heinonen

Theses and Dissertations--History

Following the first world exhibition, the 1851 Crystal Palace Exhibition in London, exhibitions became routine events across the West that merged both education and entertainment to forward political and economic goals. For the most part scholars have taken the frequency, popularity, and propagandistic efforts of exhibitions at face value, viewing them as successful reassertions of the imperial, industrial, and technological superiority of Western nation-states. Though offering valuable insights into the cultural technologies of imperial rule, these works miss the complexities of imperial projects within specific temporal and geographical contexts.

This manuscript traces the historical dynamics of India at exhibitions held …

Creating Heaven On Earth: Jim Bakker And The Birth Of A Sunbelt Pentecostalism, Eric G. Weinberg

Theses and Dissertations--History

This dissertation traces the rise of Jim and Tammy Bakker and analyzes the birth and growth of prosperity theology in the United States of America. It highlights how Jim and Tammy created a form of Pentecostalism that grew alongside and because of the growth of the Sunbelt. It blossomed in the new suburban enclaves of this region. Jim Bakker's religious ideas had their roots in an increasingly powerful anti-New Deal coalition that was led by the conservative business community. Positive thinking and the prosperity gospel reinforced their beliefs in unfettered markets and their opposition to activist government. Bakker combined these …

Literary Africa: Spanish Reflections Of Morocco, Western Sahara, And Equatorial Guinea In The Contemporary Novel, 1990-2010, Mahan L. Ellison

Theses and Dissertations--Hispanic Studies

This dissertation analyzes the strategies that Spanish and Hispano-African authors employ when writing about Africa in the contemporary novel (1990-2010). Focusing on the former Spanish colonial territories of Morocco, Western Sahara, and Equatorial Guinea, I analyze the post-colonial literary discourse about these regions. This study examines the new ways of conceptualizing Africa that depart from an Orientalist framework as advanced by the novelists Lorenzo Silva, Concha López Sarasúa, Ramón Mayrata, María Dueñas, Fernando Gamboa, Montserrat Abumalham, Javier Reverte, Alberto Vázquez-Figueroa, and Donato Ndongo. Their works are representative of a recent trend in Spanish letters that signals a literary focus on …

Specters Of The Unspeakable: The Rhetoric Of Torture In Guatemalan Literature, 1975-1985, William Jarrod Brown

Theses and Dissertations--Hispanic Studies

This dissertation examines the ways in which torture was imagined and narrated in Guatemalan literature during the Internal Armed Conflict. For nearly four decades, Guatemala suffered one of the longest and most violent wars in Latin America. During that time, it is estimated that more than 100,000 people were tortured at the hands of the Guatemalan military. Torture, as suggested by Ariel Dorman, is most fundamentally “a crime committed against the imagination” (8), disrupting and often dissolving the boundaries between fact and fiction, the real and the unreal. The Introduction and Chapter One of this study explore the destabilization of …

Deconstructing An Icon: Fidel Castro And Revolutionary Masculinity, Krissie Butler

Theses and Dissertations--Hispanic Studies

The goal of this project is to investigate the way in which various representations of Fidel Castro, between the years 1957-1965, have left an indelible mark on Cuba, transforming its landscape, I argue, through gendered means and conscious strategies. Thus it is less concerned with Fidel as an historical person than with examining with a gendered lens the ways in which he has been represented in foundational photographs, interviews, songs, and texts (both narrative and poetry as well as blogs). Drawing from theories of masculinity, which conceive masculinity as both a social construction and material body, my dissertation explores the …

Inflationary Truth-Theoretic Semantics, Michael Brady Horton

Theses and Dissertations--Philosophy

I argue that satisfaction and reference—and therefore, truth—are multiply realizable properties. I advocate a novel approach motivated by a commitment to the robustness and fruitfulness of truth-theoretic approaches to natural language semantics.

DEFLATIONISM: Philosophers keen on deflating the metaphysical pretensions of truth theories claim that we need not appeal to a substantive truth-property. Recently, however, some philosophers have sought to combine deflationism about truth with the view that our concept of truth or the truth-predicate can play an important role in natural language semantics.

TRUTH-THEORETIC SEMANTICS: The goal of a formal semantic theory of a natural language is to provide …

Cultivating The Compass: Examining The Role Of Emotional Appraisal And Professional Agency Among Stakeholders In Kentucky Agricultural Education, Savannah Faye Robin

Theses and Dissertations--Community & Leadership Development

Agricultural Education has been informed by four major areas including agricultural education (teaching and learning), educational policy, agricultural policy (industry collaboration) and research. Historically agriculture teachers have been removed from the policy process affecting their profession in these four areas (Thompson, 1963). A review of historical literature suggests that only twice have teachers been involved in the policy process. The purpose of this study was to examine the involvement levels of stakeholders in agricultural education across the state of Kentucky. Specifically, examining the emotional appraisal of specific issues in agricultural education and if the emotions of stakeholders influence their involvement …

Evaluation Of Heat Shock Protein 70a (Hsp70a) In Chlamydomonas Reinhardtii, Sarah Nicole Short

Theses and Dissertations--Biosystems and Agricultural Engineering

Algae are being considered as a possible tool for carbon dioxide mitigation because they uptake carbon dioxide during photosynthesis. Using flue gas from a coal-fired power plant as a carbon source would allow the algae to remove CO₂ from the flue gas before it is emitted into the atmosphere. Because algae do not grow well at the high temperature, low pH conditions presented by flue gas, the traditional approach has been to alter the flue gas to suit the needs of the algae; however, this work aimed to genetically modify the algae Chlamydomonas reinhardtii to grow better at less …

Ultrasonic Concentration Of Microorganisms, Samuel J. Mullins

Theses and Dissertations--Biosystems and Agricultural Engineering

Concentration of microorganisms from a sample volume would increase the limits of detection of samples used for rapid-detection methods. Rapid detection methods are is advantageous for the food industry to rapidly test for bacteria in order release products on a timely basis. Ultrasonic concentration was considered a promising method for manipulation of microorganisms. An ultrasonic chamber consisting of parallel piezoceramic discs with a reticulated polyurethane foam mesh was used to concentrate Saccharomyces cerevisiae yeast and Escherichia coli bacteria. The concentration of yeast was seen to increase by 200% (from 8.0 x 10⁴ cells mL^-1 to 2.4 x 10 …

Evaluation Of Separation Method Additives For The Recovery Of Bacteria From Food Matrices, Jennifer Leanne Frederick

Theses and Dissertations--Biosystems and Agricultural Engineering

The microbiological testing of foods is a well-established science. Due to the severity of foodborne pathogen illnesses, the widespread use and implementation of rapid detection methods in food testing labs is increasingly important. The first step for successful testing is sampling. Surfactants have been highly used in food microbiology, but there is not much, if any, published research about the use of fatty alcohols and chemical dispersants as aids in microbial separation. The microbial extraction efficiency of Escherichia coli K12 and Listeria innocua from hot dogs, spinach, and milk was measured using chemical additives (surfactants, fatty alcohols, and a chemical …

Regulation Of Hepatic Gene Expression During Liver Development And Disease, Hui Ren

Theses and Dissertations--Microbiology, Immunology, and Molecular Genetics

My first project was to investigate the role of Hepatocyte Nuclear Factor 1 (HNF1) and Nuclear Factor I (NFI) on alpha-fetoprotein (AFP) promoter activity during liver development. AFP is highly expressed in the fetal liver, silenced at birth, and remains at very low levels in the adult liver. A GA substitution located at -119 of the human AFP promoter is associated with hereditary persistence of AFP (HPAFP) expression in the adult liver (Hum Molec Genet, 1993, 2:379). The -120 region harbors overlapping binding sites for HNF1 and NFI. While it has been shown that the GA substitution increases HNF1 binding, …

The Role Of Intestinal Epithelial Cells And The Regulation Of The Polymeric Immunoglobulin Receptor In Homeostasis And Inflammation, Aubrey Leigh Frantz

Theses and Dissertations--Microbiology, Immunology, and Molecular Genetics

The mammalian intestine harbors an estimated 100 trillion microorganisms, which normally maintain a mutually beneficial relationship with the host. The intestinal epithelium consists of a single layer of intestinal epithelial cells (IECs) that provides a physical barrier as well as innate immune defense, preventing this vast community of microbes from entering host tissues. Secretory immunoglobulin A (SIgA) acts as the first line of antigen-specific immunity at the interface between the gut microbiota and the intestinal epithelium. Polymeric IgA secreted by plasma cells in the intestinal lamina propria is transported across IECs by the polymeric immunoglobulin receptor (pIgR). Defects in epithelial …

Insights Into Hepatic Alpha-Fetoprotein Gene Regulation During Liver Development And Disease, Erica Leigh Clinkenbeard

Theses and Dissertations--Microbiology, Immunology, and Molecular Genetics

The liver is an essential organ for cholesterol homeostasis. If this process becomes dysregulated, cardiovascular disease (CVD) develops. Zinc-fingers and homeoboxes 2 (Zhx2) as an important hepatic gene regulator and contributes to CVD. BALB/cJ mice, with mutant Zhx2 allele, have fewer atherosclerotic plaques compared to other strains on a high fat diet. In my dissertation, I focused on the liver phenotype in BALB/cJ mice on a high-fat diet and found increased liver damage compared to wild-type Zhx2 mice. These data indicates that reduced Zhx2 in the liver leads to CVD resistance, but increases liver damage. Therefore, Zhx2 has an important …

The Linkage Between Transcription Control And Epigenetic Regulation: The Snail Story And Beyond, Yiwei Lin

Theses and Dissertations--Pharmacology and Nutritional Sciences

Epigenetic deregulation contributes significantly to the development of multiple human diseases, including cancer. While great effort has been made to elucidate the underlying mechanism, our knowledge on epigenetic regulation is still fragmentary, an important gap being how the diverse epigenetic events coordinate to control gene transcription. In the first part of our study, we demonstrated an important link between Snail-mediated transcriptional control and epigenetic regulation during cancer development. Specifically, we found that the highly conserved SNAG domain of Snail sequentially and structurally mimics the N-terminal tail of histone H3, thereby functions as a molecular “hook”, or pseudo substrate, for recruiting …

A Diet Enriched In Stearic Acid Protects Against The Progression Of Type 2 Diabetes In Leptin Receptor Deficient Mice (Db/Db), Valerie Lynn Reeves

Theses and Dissertations--Physiology

Dietary saturated fat intake contributes to diabetes and cardiovascular disease, as shown in numerous animal and human studies. However, the hypothesis that stearic acid, a saturated fat, has beneficial effects on these conditions has not been adequately tested. Leptin receptor deficient mice (db/db) and wild-type mice were fed either chow or a high fat diet enriched in either stearic acid or oleic acid for ten weeks. The progression of diabetes was evaluated with blood glucose, insulin, and metabolic parameter measurements. At the conclusion of the study, pancreatic islet organization was examined, and blood, liver and feces were assayed for fatty …