Digital Commons Network^™

The Alchemy Of Jim Crow And Chinese Exclusion: A Study Of Chinese Mississippian Experience From Reconstruction To The Civil Rights Movement, 1870-1967, Sixu Liu

Theses and Dissertations--History

This dissertation examines the history of the Chinese community in Mississippi from the Reconstruction era to the late 1960s. Despite the modest size of their population, the experience of Chinese Mississippians helps us answer a number of key questions regarding two of America’s most impactful institutions, Chinese exclusion and Jim Crow, and their recipients. Why did Chinese Mississippians come to a state that was known for racial hatred and racial oppression? How should a people that was neither Black nor White situate themselves in a society dictated by a Black-White binary? How did Mississippi, a state that was often not …

Targeting Prmt5 In Inflammation And Cancer: A Nexus Of Endotoxin Signaling, Antigen Presentation And Immune Evasion., Jimmy James Msemo

Theses and Dissertations--Medical Sciences

Protein Arginine Methyltransferase 5 (PRMT5) is a member of PRMT family that controls both intrinsic and extrinsic oncogenic processes [1]. Protein arginine methyltransferase 5 (PRMT5) is a type II methyltransferase that exerts widespread influence over gene expression, splicing, DNA repair, and immune regulation through symmetric dimethylation of arginine residues on histone and non-histone proteins [2]. The addition of methyl groups induces gene silencing through producing dictatorial histone marks like H2AR3ne2s, H3R8me2s and H4R3mes [3], which act as epigenetic repressive marks on gene promoters. In non-histone proteins, PRMT5 can methylate and regulate transcription factor proteins such as p53, E2F1, and p65, …

Investigating Regulation And The Regulome Of The Nucleic Acid Binding Protein Spovg Within Borrelia Burgdorferi, Timothy C. Saylor

Theses and Dissertations--Medical Sciences

Prior studies indicated Borrelia burgdorferi SpoVG protein was regulated, SpoVG binds specifically to a number of nucleic acids, and SpoVG binds to the non-variable section of the major virulence factor vlsE. Although a direct transcriptional activator or inhibitor was not elucidated, it is shown that SpoVG is regulated based on cell culture density independent of the growth rate of B. burgdorferi, indicating that SpoVG regulation is outside the scope of the alternative sigma factor RpoS. Using a dCas9 CRISPRi construct to target SpoVG resulted in protein expression trending towards upregulation of the BosR-RpoS regulon, and a marked decrease …

Changes In Cancer Diagnosis And Survival In The United States During The Covid-19 Pandemic, Justin T. Burus

Theses and Dissertations--Epidemiology and Biostatistics

The COVID-19 Pandemic led to global societal disruptions as political leaders and public health authorities attempted to control the spread of the newly discovered SARS- CoV-2 virus. While these measures were designed to lessen morbidity and mortality from a novel pathogen, their impact was also felt in many other, often unintended ways. The purpose of this dissertation is to use cancer surveillance research methods to examine the association between COVID-19 Pandemic-related disruptions and changes in the normal diagnosis and care of cancer in the United States.

The first two studies of this dissertation analyzed reductions in cancer diagnoses in the …

Using Hyperactive Shp-2 Mutants, A Phosphatase, To Identify Interactions With The Rna Helicase Ddx3, Devin Montgomery

Theses and Dissertations--Pharmacy

Protein-protein interactions are critical in cellular signaling pathways. This project investigates binding interactions between SHP-2, a tyrosine phosphatase, and DDX3, an RNA helicase. There is a specific focus on the impact that gain-of-function SHP-2 mutations may impact binding to DDX3 via conformational change. Using immunoprecipitation protocols as well as western blotting we were able to provide insights into SHP-2 molecular mechanisms and subsequent binding to DDX3.

Simplified Molecular Diagnostics Methods For Environmental Testing Of Pathogens And Other Applications, Mohammad Dehghan Banadaki

Theses and Dissertations--Mechanical and Aerospace Engineering

Environmental Surveillance (ES) has emerged as a powerful tool to monitor the emergence and persistence of a pathogen within a facility or community using wastewater/water analysis. Over the past four years, implementation of ES has experienced an explosive acceleration, driven by efforts to monitor the spread of SARS-CoV-2, which has made ES a routinely used public health tool in many locations. We can now use ES to measure the emergence/presence/abundance of different viruses (e.g., SARS-CoV-2, influenza, mpox), bacteria (e.g., E. coli, V. cholerae, antibiotic-resistant strains), fungi (e.g., C. auris), and other biological markers indicative of infectious diseases. However, its broad …

Engineering A Novel Solvent Regenerator That Leverages Electrochemically Induced Ph Swings For Direct Air Capture And Hydrogen Generation, Emmanuel Aluhunmile Ohiomoba

Theses and Dissertations--Mechanical and Aerospace Engineering

Direct Air Capture (DAC) is considered to be a key component for achieving global net-negative emissions. However, to capture CO₂ at the low concentrations in the atmosphere requires materials with high affinity for CO₂. This makes the solvent-based chemical approach for DAC more applicable compared to physical approaches. Nonetheless, regenerating the capture solvent presents a significant challenge due to high heat requirements for thermal decomposition of carbonates. Leveraging electrochemical principles provides an opportunity to simplify the regeneration process, thus, this work focuses on engineering an electrochemical solvent regenerator for recovering alkaline solvents for DAC, with co-production of …

Cultivating Hegemony: Cultural Engineering And The Art Of Power Maintenance In Military Movies, Samuel Lockridge

Theses and Dissertations--Arts Administration

This research explores how the United States military’s assistance to film productions can be better classified and understood as a form of cultural policy. There is a significant gap in the existing cultural policy research concerning interventions into the arts and culture by the military aimed at influencing the thoughts, beliefs, and attitudes of the population. The degree to which the U.S. Department of Defense’s (DoD) long partnership with the film industry should even be considered “cultural policy” remains an open, and largely unexamined question. This dissertation presents an exploratory multiple case study of two films which received DoD production …

The Dying Swan: How To Predict And Evaluate The Survival Rate And Longevity Of Small Dance Companies In The United States, Evemarie N. Bessenbach

Theses and Dissertations--Arts Administration

Smaller dance organizations are an integral component of the arts and culture sector in the United States, making valuable artistic, economic, and social contributions despite limited resources. They provide employment for professional artists, contribute to a region’s economic vitality, and increase accessibility to quality arts-related programming. However, there are numerous professional dance companies that have permanently closed these past ten years alone, such as Dance Theatre of Tennessee, Ballet Theatre of Indiana, and Missouri Ballet Theatre, to just name a few—all of which can be considered smaller dance organizations.

The purpose of this interdisciplinary, exploratory study is to address the …

Impact Of Hyperthermia And Mitomycin C On Heat Shock Proteins, Mismatch Repair Proteins, And Cytotoxicity In Colon Cancer, Anna M. Reagan

Theses and Dissertations--Clinical and Translational Science

Cytoreductive surgery and heated intraperitoneal chemotherapy (HIPEC) is a treatment used for primary peritoneal malignancy or metastases, including colon cancer. We aimed to explore the effects of hyperthermia and/or mitomycin C (MMC) on mismatch repair (MMR) proteins and heat shock proteins (hsps) and the potential for post-HIPEC treatment with pembrolizumab treatment. Colon cancer cells were treated with hyperthermia and/or MMC for 90 minutes. Subcellular fractionation and immunofluorescent imaging were performed to identify protein abundance and localization of MSH2, MLH1, hsp70, and hsp27. Proteomics analysis was performed for HT29 cells. HT29 cells and patient-derived organoids (PDOs) underwent simulated HIPEC and treatment …

Exploring Healthcare Access In Telehealth Through Spanish-Speaking Patients’ Perspectives: A Scoping Review, Abigail J. Verst

Theses and Dissertations--Communication Sciences and Disorders

Background: Telehealth offers a potential solution to addressing access barriers experienced by Spanish-speaking patients. This scoping review aimed to explore whether a comprehensive view of healthcare access is being considered when creating telehealth programs for Spanish-speakers. The Conceptual Framework of Access to Healthcare (Levesque et al., 2013) was used to guide access analysis.

Methods: A literature search was conducted across three databases to identify empirical studies in the United States that reported Spanish-speaking patients’ perspectives of telehealth through quantitative questions. Data was collected from the included articles to characterize the type of healthcare providers and telehealth modalities examined in the …

Holding Space For Two Truths: Thematic Analysis Of Speech Language Pathologists’ And Teachers’ Philosophies And Practices In Reading Comprehension, Ryan Foote

Theses and Dissertations--Communication Sciences and Disorders

Introduction: Reading is a doorway to life participation. Only about one-third of fourth graders have read at grade level for the last three decades. Multiple views on what constitutes skilled reading emphasize decoding and greater variability among comprehension-related skills. Scarborough’s Reading Rope is the most specific in defining discrete skills necessary for adequate reading comprehension. This study aims to understand how elementary-level general education teachers and speech-language pathologists define “reading comprehension” and describe their practices in assessing and developing these skills. Methodology: Elementary school speech-language pathologists and teachers were interviewed about their conceptualizations and applications of the discrete skills in …

Practicing Clinicians' Views Of The Life Participation Approach To Aphasia Therapy, Stephanie H. Mays

Theses and Dissertations--Communication Sciences and Disorders

The Life Participation Approach to Aphasia therapy (LPAA) aligns with a person-centered, functional approach to speech therapy. Past research has demonstrated that persons with aphasia (PWA) benefit from this approach and prefer it when compared to the traditional medical model to therapy. However, a disconnect between PWA preferences and SLP treatment approaches in therapy persists. This study aims to identify both barriers and facilitators that SLPs face when implementing LPAA. Five CCC-SLPs practicing in the state of KY with experience working with PWA were recruited as participants. Participants were placed into two focus groups, with each group meeting for 2-3 …

M1 Macrophage-Engineered Vesicles Have Anti-Cancer Activity In Ovarian Cancer, Connie Du Cao

Theses and Dissertations--Clinical and Translational Science

Ovarian cancer typically presents at an advanced stage, has a poor prognosis, and is a leading cause of cancer-related deaths in women. Extracellular vesicles (EVs) are cell-derived membrane-bound nanoparticles that function in specific cell-to-cell communication and are under development as novel drug delivery vehicles and modulators of the tumor microenvironment. Artificial cell-derived vesicles (ACDVs) derived from M1 macrophages are able to repolarize M2 macrophages to the M1 phenotype and target tumor cells in in vitro studies.

In this study, we generated engineered EVs (EEVs) by membrane disruption of M1 macrophages (MEVs) in the presence and absence of cisplatin to generate …

Assessing The Effectiveness Of A Modified Cook Together Eat Together Program On Food Resource Management Behaviors In A Rural Setting, Johanna K. Poysti

Theses and Dissertations--Nutrition and Food Systems

This study evaluated the effectiveness of a modified, hybrid Cook Together, Eat Together (CTET) program on food resource management behaviors in a rural setting. A six-week intervention combining virtual and in-person sessions aimed to improve health behaviors, food resource management skills, and self-efficacy. Although most behavioral changes were not statistically significant, participants reported increased confidence in cooking healthy meals on a budget. Positive qualitative feedback from focus group discussions indicated program feasibility and engagement. Strengths and limitations of the study were discussed as well as recommendations for future research were given.

Bed Bugs: Health Risks And Control, Simona Principato

Theses and Dissertations--Entomology

Bed bugs are blood-feeding hemipterans strongly associated with humans. Their detection in indoor environments, such as homes, hotels, trains, and public facilities, has increased gradually over the past 20 years. The exact cause of their resurgence is still unclear, but the increase of international travel and insecticide resistance are thought to have contributed to it.

Although bed bugs are not known to transmit diseases, the EPA, CDC, and USDA have classified them as a pest of significant public health concern, mainly due to the dermatitis that they can cause. Beyond the dermatological burden of the bites, other health implications often …

The Role Of Nutrition In Managing Honey Bee Viral Pathogen, Caroline Wylie Kane

Theses and Dissertations--Entomology

This study evaluated the impact of nutritional supplements on viral infections in honey bee colonies, focusing on Lake Sinai Virus 2 (LSV2), Deformed Wing Virus A (DWVA), and Deformed Wing Virus B (DWVB). I partnered with 32 hobbyist beekeepers across Kentucky, who volunteered 138 colonies for a month-long field study. Half of the colonies received weekly commercial supplements, while the other half served as controls. Viral titers were measured before and after the supplementation period using qPCR. Supplements did not significantly reduce viral titers for any of the three viruses, suggesting that short-term supplementation may not be effective in lowering …

Engineering Novel Biomimetic Models Of Equine Host-Microbe Interactions, Lynn M. Leedhanachoke

Theses and Dissertations--Veterinary Science

One major limitation to the study of equine disease is the lack of in vitro models that accurately reflect the dynamic physiology of the horse. Thus, there is a pressing need to develop in vitro systems that recapitulate diverse equine tissue and organ microenvironments. To overcome this deficiency, we developed multiple biomimetic equine organoid systems that can be employed for a variety of investigations aimed at resolving outstanding questions in infectious disease pathogenesis, therapeutic development and drug metabolism, host defense mechanisms, immune-modulated disease, and equine developmental biology. Our innovative platform incorporates novel equine intestinal enteroid and airway organoid models derived …

Does Participation In An Innovative Livestock Exhibition Program Foster The Development Of Grit In Youth Across Rural, Micro, And Metropolitan Communities?, Reba V. Prather

Theses and Dissertations--Community & Leadership Development

This research examined whether livestock exhibition could be used to foster grit in youth across rural, micropolitan, and metropolitan communities. More specifically, the researcher sought to evaluate the impact an innovative livestock exhibition program had on youth resilience. Through partnerships with 4-H clubs and agricultural organizations, the initiative encourages community-driven involvement, allowing youth to take ownership of their growth and development. The program utilized agricultural animal husbandry experiences as a mechanism for youth development, exploring the broader impact of livestock engagement on character-building. By assessing the outcomes of these experiences, the research highlights the trials and tribulations associated with fostering …

Mapping The Impact Of Leadership Training: A Ripple Effects Mapping Of The Kentucky Extension Homemaker Association’S First Leadership Academy, Kelly May

Theses and Dissertations--Community & Leadership Development

This research study evaluates the first Kentucky Extension Homemaker Association (KEHA) Leadership Academy using the participatory, qualitative evaluation tool Ripple Effects Mapping (REM). This study explored the impact and outcomes of the training, both intended and unintended.

REM is a participatory evaluation method that begins with appreciative inquiry, then continues with a group discussion where stories are “mapped” or illustrated in real time. Using the Theming and Rippling Method of REM, the research team coded the information on site with inductive theming and those themes were then shared and confirmed with the group. After the event, the mapped data was …

Impacts Of Utilizing Reverse Osmosis Concentrate On Mashing, Fermentation, And Water Use In Bourbon Whiskey Production, Ryan C. Sarhan

Theses and Dissertations--Biosystems and Agricultural Engineering

This thesis addresses the feasibility of reusing reverse osmosis concentrate (ROC) in the bourbon whiskey fermentation processes. Initial work compared laboratory-scale mashing and fermentation methods, demonstrating that simultaneous saccharification and fermentation reliably reflects industrial-scale processes, with lower complexity and process time. Building upon this, laboratory and distillery-scale experiments demonstrated that reusing ROC in the mashing and fermentation steps does not negatively impact ethanol yields. ROC produced by systems with a water softener was found to significantly increase mash rheology, suggesting a need for potential process adjustments. Softened ROC also altered the production of flavor-influencing volatile congeners. Under worst case conditions, …

Development Of A Cultivation System For Producing Pure Mycelium Materials From Distillers' Spent Grains, Keya Rani Roy

Theses and Dissertations--Biosystems and Agricultural Engineering

Stillage is a byproduct of distilleries which is rich in organic matter, minerals, and acidic components. It is commonly used as animal feed and has high potential for use as an alternative substrate for microorganisms. Filamentous fungi are saprophytes that can utilize stillage solids to grow as threadlike mycelium. The structure and composition of the filamentous mycelium has shown promise to produce pure mycelium materials (PMM), which might have many potential applications, for instance as a leather-like material or alternative protein food ingredient. Basidiomycota fungi (including true mushrooms) species are presently used in industry to produce PMM due to the …

Utilitzing A Nurse Triage Model To Decrease The Administrative Burden Of Emr Messaging, Pamela B. Schneider

DNP Projects

Abstract

Background: The volume of Electronic Medical Records (EMR) messages from patients to providers continues to rise, yet clinic workflows have not been adequately restructured to accommodate. Increased EMR, especially the inbasket, is associated with provider burnout and decreased job satisfaction, contributing to provider turnover.

Purpose: To improve the triage and distribution of the EMR messaging workload

Method: A Nurse Triage Flowchart was implemented in the Gastroenterology Clinic at the University of Kentucky to help RNs/MAs triage patient messages for improved workflow. Surveys were completed by RNs/MAs and providers before and three months post-implementation. Data from EPIC over six …

Evaluating A Public Health Program Using The Cdc Framework: A Case Study Approach, Brooke A. Poe

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

In Bourbon County, Kentucky, where rural health challenges and chronic disease burdens persist, a coalition of public health partners set out to improve population-level physical activity through an innovative, community-centered initiative. The Bourbon County Physical Activity Program emerged through collaboration between the Bourbon County Health Department, the University of Kentucky College of Public Health, and UK HealthCare. Together, these partners designed a six-month intervention that combined discounted YMCA memberships, partnerships with employers, a physical activity tracking app with behavioral incentives, and community-wide events to promote accessible and sustained engagement.

Guided by the CDC’s framework for program evaluation in public health, …

Patterns Of Inpatient Antibiotic Utilization By Race And Ethnicity At Us Children’S Hospitals, Bethany A. Wattles, Jeffrey I. Campbell, Theresa Kluthe, Yana B. Feygin, Kahir Jawad, Michelle D. Stevenson, Deborah Winders Davis, Jennifer Porter, V. Faye Jones, Matt Hall, Michael J. Smith

Biostatistics Faculty Publications

Background and Objectives: Racial and ethnic variations in antibiotic utilization are well-reported in outpatient settings but little is known about inpatient settings. Our objective was to describe national inpatient antibiotic utilization among children by race and ethnicity. Methods: This study included hospital visit data from the Pediatric Health Information System between 01/01/2022 and 12/31/2022 for patients <20 years. Primary outcomes were the percentage of hospitalization encounters that received an antibiotic and antibiotic days of therapy (DOT) per 1000 patient days. Mixed-effect regression models were used to determine the association of race-ethnicity with outcomes, adjusting for covariates. Results: There were 846,530 hospitalizations. 45.2% of children were Non-Hispanic (NH) White, 27.1% were Hispanic, 19.2% were NH Black, 4.5% were NH Other, 3.5% were NH Asian, 0.3% were NH Native Hawaiian/Other Pacific Islander (NHPI) and 0.2% were NH American Indian. Adjusting for covariates, NH Black children had lower odds of receiving antibiotics compared to NH White children (aOR 0.96, 95%CI 0.94–0.97), while NH NHPI had higher odds of receiving antibiotics (aOR 1.16, 95%CI 1.05–1.29). Children who were Hispanic, NH Asian, NH American Indian, and children who were NH Other received antibiotic DOT compared to NH White children, while NH NHPI children received more antibiotic DOT. Conclusions: Antibiotic utilization in children’s hospitals differs by race and ethnicity. Hospitals should assess policies and practices that may contribute to disparities in treatment; antibiotic stewardship programs may play an important role in promoting inpatient pharmacoequity. Additional research is needed to examine individual diagnoses, clinical outcomes, and drivers of variation.

Valorizing Municipal Solid Waste For Sustainable Aviation Fuel And Polyhydroxyalkanoate Bioplastics: Pyrolysis And Microbial Pathways, Emon Das

Theses and Dissertations--Biosystems and Agricultural Engineering

The escalating global burden of municipal solid waste (MSW), projected to reach 3.88 billion tons annually by 2050, calls for innovative valorization strategies to mitigate environmental impacts and promote a circular bioeconomy. This thesis investigates the conversion of MSW into two high-value products: sustainable aviation fuel (SAF) and polyhydroxyalkanoate (PHA) bioplastics. Chapter one presents comprehensive physicochemical characterization and pyrolysis-GC/MS analysis of MSW feedstocks. The physicochemical results reveal that densification enhances handling and storage properties, while pyrolysis-GC/MS analysis indicates that plastic-rich MSW streams produce bio-oils with favorable hydrocarbon profiles suitable for SAF production, potentially contributing to decarbonization efforts in the aviation …

Serglycin Controls Megakaryocyte Retention Of Platelet Factor 4 And Influences Megakaryocyte Fate In Bone Marrow, Joshua Lykins, Isabelle C. Becker, Virginia Camacho, Hammodah Rawhi Hammodah Alfar, Joonwoo Park, Joseph Italiano, Sidney W. Whiteheart

Saha Cardiovascular Research Center Faculty Publications

Megakaryocytes (MKs) produce platelets, and similar to other hematopoietic progenitors, they are involved in homeostatic aspects of their bone marrow niche. MKs release and endocytose various factors, such as platelet factor 4 (PF4)/CXCL4. Here, we show that the intra-α-granular proteoglycan, serglycin (SRGN), plays a key role in this process by retaining PF4, and perhaps other factors, during MK maturation. Immature, SRGN–/– MKs released ~80% of their PF4, and conditioned media from these cells negatively affected wild-type MK differentiation in vitro. This was replicated in wild-type MKs by treatment with the polycation surfen, a known inhibitor of glycosaminoglycan (GAG)/protein interactions. In …

El Doble Discurso Del Simbolismo De La (Pureza De) Sangre En El Diario Espiritual De La Donada Afroperuana Úrsula De Jesús, Yertty M. Vandermolen

Theses and Dissertations--Hispanic Studies

Purity of Blood Symbolism and Double Discourse in The Spiritual Diary of the Afro-Peruvian Donada Úrsula de Jesús

Through a close reading of The Spiritual Diary, this study examines how the Afro-Peruvian donada Úrsula de Jesús—whose experiences and visions were recorded and transcribed by a group of literate nuns in her convent—employs discursive strategies to dismantle the discourse of blood purity and reformulate the meaning of the blood. Drawing on the Christian belief that all humans are created equally in the image of God and redeemed through the blood of Christ as promised for universal salvation, Úrsula constructs an egalitarian …

“It’S An Honor To Be In This Body”: A Black Feminist Grounded Theory Of Embodiment Among Black Womxn Yoga Practitioners (Bwyps), Natalie Malone

Theses and Dissertations--Educational, School, and Counseling Psychology

Black womxn (i.e., individuals whose experiences align with Black girlhood/womanhood or whose bodies are perceived as Black, female, womanly, femme, or a combination) experience significant health and well-being challenges that may hinder their embodiment. Yoga is a complementary therapy that may help Black womxn improve their embodiment. This dissertation qualitatively explores the processes Black womxn yoga practitioners (BWYPs) engage in to cultivate positive embodiment. Participants were N=30 Black womxn who were short- (≤ five years; n=11), medium- (five–10 years; n=10), and long-term (>11 years; n=9) yoga practitioners. Participants completed individual interviews as part of the …

Addressing Municipal Road Aid Funding In Kentucky During The Era Of Electric Vehicles, Jacob Bruce, Tyler Chapman, Blake Girdler

MPA/MPP/MPFM Capstone Projects

The rapid rise of electric vehicles (EVs) poses a growing threat to Kentucky’s Municipal Road Aid Fund, which relies heavily on fuel tax revenues. As EV adoption increases and fuel consumption declines, the existing funding model becomes increasingly unsustainable, jeopardizing road maintenance, infrastructure development, and municipal services across the state. Commissioned by the Kentucky League of Cities, this study investigates viable policy solutions to ensure long-term fiscal stability for municipal road funding in an electrifying transportation landscape. Drawing from comparative analyses of peer and neighboring states, the report evaluates alternative funding mechanisms such as road usage charges, EV-specific registration fees, …