Digital Commons Network^™

Biological Selenium Control: Selenium Reduction By Shigella Fergusonii Strain Tb42616 And Pantoea Vagans Strain Ewb32213-2 In Bioreactor Systems, Yuxia Ji

Theses and Dissertations--Civil Engineering

Se(VI) and Se(IV), as the two major species of selenium in water, are toxic to aquatic lives and may cause adverse health effects to humans at high levels. Biological reduction of Se(VI) is a two-stage process first from Se(VI) to Se(IV) and then from Se(IV) to Se(0) with potential accumulation of the more toxic Se(IV) due to the slower rate of the second stage.

Selenium reduction was first evaluated with batch cultures of Shigella fergusonii strain TB42616 (TB) and Pantoea vagans strain EWB32213-2 (EWB) isolated in our laboratory from sludge and coal slurry sediment samples, respectively. In order to facilitate …

Variability In Groundwater Flow And Chemistry In The Houzhai Karst Basin, Guizhou Province, China, Joshua M. Barna

Theses and Dissertations--Earth and Environmental Sciences

Understanding how karst aquifers store and transmit water and contaminants is an ongoing problem in hydrogeology. Flowpath and recharge heterogeneity contribute to the complexity of these systems. This thesis explores karst-conduit connectivity and water chemistry variability in the Houzhai catchment in Guizhou province, China. Artificial tracer tests were conducted during both the monsoon and dry seasons to understand temporal variability in connectivity and water velocity between karst features. Multiple flowpaths through the catchment are activated during the monsoon season and partially abandoned during the dry season. Additionally, gradient reversals during monsoonal high-flow events and as a result of pumping can …

Integrated Seismic-Reflection And Microgravity Imaging Across The Southern Boundary Of The Charleston Uplift, New Madrid Seismic Zone, Usa, Drew D. Burford Jr.

Theses and Dissertations--Earth and Environmental Sciences

The Charleston Uplift (CU), a 30-km-long by 7-km-wide, N46°E-oriented subsurface geologic anomaly in the northern Mississippi embayment near Charleston, Missouri, exhibits up to 36 m of vertical relief across the Paleogene/Quaternary unconformity. Subsurface structural relief, along with the CU’s coincident boundary alignment with contemporary microseismicity and the New Madrid North Fault (NMNF), suggest a structural origin. Subsequent seismic soundings indicate vertical structural relief is present in Cretaceous and Paleozoic horizons, supporting the fault-controlled origin. The southern boundary (CU-s) had not been investigated, nor had any direct fault images been acquired. Integrated microgravity and seismic-reflection methods across the inferred CU-s establish …

Extension Of A Quaternary-Active Shear Zone Across The Reelfoot Fault Stepover Arm: Evidence From P- And Sh-Wave Seismic Reflection Imaging, Brooks Rosandich

Theses and Dissertations--Earth and Environmental Sciences

Many seismic hazard source parameters such as slip rate, total displacement, strain accommodation, geographic fault location, etc. are poorly constrained in the New Madrid seismic zone (NMSZ). This is in large part due to the masking effect of the thick Mississippi embayment sediment package on seismogenic structures and features. Consequently, much of the subsurface geologic characterization needed for understanding seismic hazard sources requires geophysical imaging. Recent seismic reflection surveys 12 km NE of the Reelfoot Fault stepover arm of the NMSZ have suggested a northeast-oriented transpressional fault zone extending across the Reelfoot Fault stepover arm where its dextral displacement at …

Setting The Stage: Resident Experiences With Enforcement, Rescue And Spectacle In Lampedusa, Elisa Sperandio

Theses and Dissertations--Geography

Located 127 miles from the shores of Sicily and only 70 from Tunisia, the island of Lampedusa is home to a population of 6000. Residents are largely reliant on a centuries-old fishing economy, a booming tourism industry and, most recently, the sustainment of a complex apparatus of border enforcement. Since the early 2000s, with the hardening of the southern border of Italy and the European Union, a multitude of actors have converged to Lampedusa: from migrants, to agents of enforcement, to NGO personnel, along with journalists, researchers and tourists. In this thesis, I center the experiences of island residents to …

Unsettling Refuge: Syrian Refugees’ Account Of Life In Denmark, Malene H. Jacobsen

Theses and Dissertations--Geography

This doctoral dissertation examines the lived experiences of refuge in Denmark from the perspectives of Syrian refugees. Situated within feminist political geography, it moves beyond examining geopolitics merely from the perspective of the law, the state, and policy makers. Instead, it seeks to grasp the ways in which geopolitics are encountered, experienced, and negotiated on the ground – by the people who are most affected by state policies and practices. It draws on more than ten months of ethnographic fieldwork in Denmark with Syrian refugees, including semi-structured interviews, focus groups, and participant observations, as well as interviews with state and …

Values In The Air: Community And Capital Conversion In The Nineteenth-Century Novel, Deirdre Mikolajcik

Theses and Dissertations--English

Values in the Air argues that nineteenth-century authors attempted to challenge the individualizing and atomizing effects of the increasingly powerful and abstract investment economy by portraying the necessity of other fields of capital (cultural, social, domestic) to the formation and maintenance of local, knowable communities. I first look at the depiction of a successful integration of diverse capitals embodied in the figure of the male mill owner, wherein the idea of land stewardship is repurposed to include factories. Chapter 2 depicts an encroaching pessimism about tradition’s ability to answer the demands of the modern industrial economy even as the possibility …

But What Has Helga Crane To Do With The West Indies? Plantation Afterlives In The Black Atlantic, Rachel Mckenzie Carr

Theses and Dissertations--English

“But What Has Helga Crane to Do with the West Indies? Plantation Afterlives in the Black Atlantic” situates the emergence of the southern gothic in modernist American and Caribbean works as a response to the shifting cultural narrative of the plantation in the twentieth century. In this project, I argue that the plantation seeps out of its place and time to haunt landscapes it may never have touched and times in which slavery is long over. While the plantation system is broadly recognized as a literary, political, and cultural force in nineteenth-century literary studies, I conceive it is also a …

Shifting Personas: A Case Study Of Taylor Swift, Lela R. Lyon

Theses and Dissertations--Linguistics

This thesis analyzes how Taylor Swift has changed the way she expresses her Southern identity, specifically her dialectal features, over the course of her career and through her switch from country music to pop music. There were two processes to assess the change in Swift’s speech: the production of /ai/ tokens in interviewed speech and the perception of dialectal change by fans in the comment sections of the interviews on YouTube. Seven interviews on YouTube and their comment sections were used as the data source for this study. Production of /ai/ was measured through an auditory analysis to determine whether …

Poéticas Minimalistas De La Ciudad Contemporánea: Iribarren, Mínguez Y Del Val, David Delgado López

Theses and Dissertations--Hispanic Studies

Throughout the Spanish poetic production of the 20^th century, cities have developed a relevant role as a recurring space at the same time as society urbanized and an exodus took place from agricultural areas to the work centers offered by the cities. Since the second half of the 19^th century the city has been the meeting place for people from different backgrounds where the poet found, from his exclusive point of view, a new universe to develop in his work. However, the evolution of capitalist society sponsored the poet's transition from an artist to a worker in the …

An Inverse Eigenvalue Problem For The Schrödinger Equation On The Unit Ball Of R3, Maryam Ali Al Ghafli

Theses and Dissertations--Mathematics

The inverse eigenvalue problem for a given operator is to determine the coefficients by using knowledge of its eigenfunctions and eigenvalues. These are determined by the behavior of the solutions on the domain boundaries. In our problem, the Schrödinger operator acting on functions defined on the unit ball of $\mathbb{R}^3$ has a radial potential taken from $L^2_{\mathbb{R}}[0,1].$ Hence the set of the eigenvalues of this problem is the union of the eigenvalues of infinitely many Sturm-Liouville operators on $[0,1]$ with the Dirichlet boundary conditions. Each Sturm-Liouville operator corresponds to an angular momentum $l =0,1,2....$. In this research we focus on …

Investigating The Feasibility Of Using Mesoporous Silica Particles To Deliver Glucose During Anaerobic Fermentation, Megan E. Walz

Theses and Dissertations--Biosystems and Agricultural Engineering

The study presented herein investigated a potential low-energy method to separate and concentrate glucose from a lignocellulosic hydrolysate. The motivation for this method was twofold: 1) to provide the fermentation microorganism an optimal glucose concentration and 2) to supply a fermentation media free of inhibitory compounds. Two sizes of porous silica particles (with 7 nm and 2.3 nm pores) were synthesized and their ability to adsorb glucose from solution confirmed. Next, the ability of two different microorganisms, Saccharomyces cerevisiae and Streptococcus bovis, to utilize sugars adsorbed to the porous silica particles was investigated.

Both the 7 nm and 2.3 …

Rapid No• Measures In Rat Nucleus Accumbens And Frontal Cortex Following Nasal Administration Of Nitroglycerin, Victoria A. Scott

Theses and Dissertations--Medical Sciences

Nitric Oxide (NO) is a powerful endogenous free radical that has numerous biological functions including vasodilation and serves as a post synaptic second messenger in the central nervous system (CNS). Numerous studies implicate NO• involvement in CNS disorders such as schizophrenia and drug abuse. These studies address the direct in vivo determination of an FDA-approved NO• donor (nitroglycerin) on extracellular levels of NO• in the frontal cortex and core of the nucleus accumbens in a lightly anesthetized rat. State-of-the-art in vivo amperometric recording techniques coupled with a novel 4-channel low noise pre-amplifier system and new generation microelectrode arrays (MEAs) will …

The Stimulation Of Dendritic Cells By Cationic Lipids, John Peyton Bush

Theses and Dissertations--Medical Sciences

The discovery that cationic lipids can independently stimulate the immune system has generated interest in their potential as vaccine adjuvants. Here, we show that the cationic lipid R-DOTAP can independently stimulate type 1 interferon production in dendritic cells in both primary culture and immortalized cell culture. Levels of type 1 interferon production are cell line-dependent and limited in vitro by lipid-induced cell death. We show that cationic lipids can independently activate TLR-7 and TLR-9, suggesting a mechanism for type 1 interferon induction. This TLR-stimulatory activity is not restricted to R-DOTAP and can be extended to other similar cationic lipids in …

Essays On External Forces In Capital Markets, Marcus Painter

Theses and Dissertations--Finance and Quantitative Methods

In the first chapter, I find counties more likely to be affected by climate change pay more in underwriting fees and initial yields to issue long-term municipal bonds compared to counties unlikely to be affected by climate change. This difference disappears when comparing short-term municipal bonds, implying the market prices climate change risks for long-term securities only. Higher issuance costs for climate risk counties are driven by bonds with lower credit ratings. Investor attention is a driving factor, as the difference in issuance costs on bonds issued by climate and non-climate affected counties increases after the release of the 2006 …

Gay Men And Satisfaction With Health Care Interactions, Michael Huggins

Theses and Dissertations--Nursing

The purpose of this research was to determine relationships among depression, anxiety, self-rated physical and mental health, self-advocacy, internalized homophobia, and quality of patient-provider communication to satisfaction with health care interactions. These were measured while controlling for select demographic variables: age; ethnicity; urban or rural domicile; relationship status; household income; highest educational attainment; health insurance; disclosure to health care provider as a gay man; reason for last healthcare visit; and, general health self-rating. The specific aims of this study were to: 1) identify general characteristics of gay men in this sample; 2) examine how levels of satisfaction with health care …

Actinomycin Familial Diversity Driven By Phenoxazinone-Core Reactivity, Matthew Richard Mcerlean

Theses and Dissertations--Pharmacy

Actinomycins are a class of compounds consisting of phenoxazinone-like core attached to two peptidolactone rings, denoted as α and β. A unique component of a few families—actinomycins G, Y, and Z—is a chlorinated β-ring threonine residue. Families G and Y also contained an actinomycin that possess a β-ring heterocycle (actinomycins G₅ and Y₅, respectively); prior to this work, no β-ring heterocycle-containing actinomycins were reported for the Z family. Unlike other actinomycin derivatives, Y₅’s cytotoxicity was abolished while still maintaining some antibacterial potency.

We constructed a model compound to probe the physical properties of the actinomycin …

Phase Behavior Of Amorphous Solid Dispersions: Miscibility And Molecular Interactions, Kanika Sarpal

Theses and Dissertations--Pharmacy

Over the past few decades, amorphous solid dispersions (ASDs) have been of great interest to pharmaceutical scientists to address bioavailability issues associated with poorly water-soluble drugs. ASDs consist of an active pharmaceutical ingredient (API) that is typically dispersed in an inert polymeric matrix. Despite promising advantages, a major concern that has resulted in limited marketed formulations is the physical instability of these complex formulations. Physical instability is often manifested as phase heterogeneity, where the drug and carrier migrate and generate distinct phases, which can be a prelude to recrystallization. One important factor that dictates the physical stability of ASDs is …

Carbapenem-Resistant Enterobacteriaceae: Epidemiology, Genetics, In Vitro Activity, And Pharmacodynamic Modeling, Brandon Kulengowski

Theses and Dissertations--Pharmacy

Background: Infections caused by carbapenem-resistant Enterobacteriaceae (CRE) such as Escherichia coli and Klebsiella pneumoniae are among the most urgent threats of the infectious disease realm. The incidence of these infections has been increasing over the years and due to very limited treatment options, mortality is estimated at about 50%. By 2050, mortality from antimicrobial resistant infections is expected to surpass cancer at 10 million deaths annually.

Methods: We evaluated 18 contemporary antimicrobials against 122 carbapenem-resistant Enterobacteriaceae using a variety of antimicrobial susceptibility testing methods according to Clinical Laboratory Standards Institute guidelines. Time-kill studies were performed on clinical isolates with variable …

Mechanisms And Thermodynamics Of The Influence Of Solution-State Interactions Between Hpmc And Surfactants On Mixed Adsorption Onto Model Nanoparticles, Salin Gupta Patel

Theses and Dissertations--Pharmacy

Nanoparticulate drug delivery systems (NDDS) such as nanocrystals, nanosuspensions, solid-lipid nanoparticles often formulated for the bioavailability enhancement of poorly soluble drug candidates are stabilized by a mixture of excipients including surfactants and polymers. Most literature studies have focused on the interaction of excipients with the NDDS surfaces while ignoring the interaction of excipients in solution and the extent to which the solution-state interactions influence the affinity and capacity of adsorption. Mechanisms by which excipients stabilize NDDS and how this information can be utilized by formulators a priori to make a rational selection of excipients is not known.

The goals of …

Development Of Mithramycin Analogues With Improved Efficacy And Reduced Toxicity For Treatment Of Ets-Dependent Tumors In Ewing Sarcoma And Prostate Cancer, Joseph Michael Eckenrode

Theses and Dissertations--Pharmacy

Introduction: Genetic rearrangements in Ewing sarcoma, prostate, and leukemia cells result in activation of oncogenic ETS transcription factor fusions. Mithramycin (MTM) has been identified as an inhibitor of EWS-FLI1 transcription factor, a gene fusion product responsible for oncogenesis in Ewing sarcoma. Despite preclinical success, a phase I/II clinical trial testing MTM therapy in refractory Ewing sarcoma was terminated. Liver and blood toxicities resulted in dose de-escalation and sub-therapeutic exposures. However, the promise of selectively targeting oncogenic ETS transcription factors like EWS-FLI1 prompted us to undertake the discovery of more selective, less toxic analogues of MTM. MTM is a potent inhibitor …

Computational Modeling Guided Discovery Of Novel Inhibitors Of Mpges-1 And Butyrylcholinesterase As Drug Candidates, Shuo Zhou

Theses and Dissertations--Pharmacy

Ever since the advent of computer-aided drug design (CADD), in silico simulation methods have greatly accelerated the drug discovery process and lead to the discovery of numerous drug candidates. With the exponential growth of computational power, we nowadays simulate biologic systems at a scale unimaginable a decade ago and thus provides perspectives for drug design. In this dissertation research, combining in silico simulation methods like molecular docking and molecular dynamics (MD) simulation with organic synthesis, in vitro/in vivo experiments and clinical data mining, we developed new drug discovery strategies. These strategies were applied in our drug discovery projects and led …

Toward An Enzyme-Coupled, Bioorthogonal Platform For Methyltransferases: Probing The Specificity Of Methionine Adenosyltransferases, Tyler D. Huber

Theses and Dissertations--Pharmacy

Methyl group transfer from S-adenosyl-l-methionine (AdoMet) to various substrates including DNA, proteins, and natural products (NPs), is accomplished by methyltransferases (MTs). Analogs of AdoMet, bearing an alternative S-alkyl group can be exploited, in the context of an array of wild-type MT-catalyzed reactions, to differentially alkylate DNA, proteins, and NPs. This technology provides a means to elucidate MT targets by the MT-mediated installation of chemoselective handles from AdoMet analogs to biologically relevant molecules and affords researchers a fresh route to diversify NP scaffolds by permitting the differential alkylation of chemical sites vulnerable to NP MTs that are unreactive to …

Social Workers’ And Teachers’ Feelings Of Self-Efficacy In Dealing With School Bullying, Sharon Lynn Simmons

Theses and Dissertations--Social Work

Bullying continues to be a serious problem in schools. School social workers and teachers face challenges daily to deal with bullying. This author examined school social workers’ and teachers’ perceptions about their feelings of efficacy to deal with bullying and what may account for those feelings in a population of 71 teachers and 26 social workers employed in Kentucky schools. Research was gathered using a self-report, electronic survey consisting of subscales of the Teachers’ Attitudes about Bullying Questionnaire (Beran, 2005), the School Bullying Questionnaire (Nicolaides, Toda & Smith, 2002), the Toronto Empathy Questionnaire (Spreng, McKinnon, Mar & Levine, 2009) and …

Influence Of Water Injection Rate On The Vortecone, An Impingement Screen, And A Conventional Filter Screen Cleaning Efficiency, Oscar Velasquez

Theses and Dissertations--Mining Engineering

Industrial produced respirable particles are known to affect the respirable tract of workers causing serious illness or even death. These particles can be found in any mining operation’s environment, new regulations by the Mine Safety and Health Administration (MSHA) in 2014 reduced the allowable limit of dust exposure for underground workers. Dust control practices have been improving over the years to lower the number of incidents caused by these particles. In underground mining, water sprays and scrubber systems have become the most used dust control methods. Water sprays create numerous fine water droplets to capture dust once it has become …

Transforming A Circular Economy Into A Helical Economy For Advancing Sustainable Manufacturing, Ryan T. Bradley

Theses and Dissertations--Mechanical and Aerospace Engineering

The U.N. projects the world population to reach nearly 10 billion people by 2050, which will cause demand for manufactured goods to reach unforeseen levels. In order for us to produce the goods to support an equitable future, the methods in which we manufacture those goods must radically change. The emerging Circular Economy (CE) concept for production systems has promised to drastically increase economic/business value by significantly reducing the world’s resource consumption and negative environmental impacts. However, CE is inherently limited because of its emphasis on recycling and reuse of materials. CE does not address the holistic changes needed across …

Design Process For The Containment And Manipulation Of Liquids In Microgravity, Chris Meek

Theses and Dissertations--Mechanical and Aerospace Engineering

In order to enhance accessibility to microgravity research, the design process for experiments on the ISS must be streamlined and accessible to all scientific disciplines, not just aerospace engineers. Thus, a general design and analysis toolbox with accompanying best practices manual for microgravity liquid containment is proposed. The work presented in this thesis improves the design process by introducing a modular liquid tank design which can be filled, drained, or act as a passive liquid-gas separation device. It can also be pressurized, and used for aerosol spray. This tank can be modified to meet the design requirements of various experimental …

Photography, Visual Culture, And The (Re)Definition/Queering Of The Male Gaze, David Nicholas Martin

Theses and Dissertations--Art and Visual Studies

The traditional notion of the Male Gaze, first conceptualized by feminist film critic Laura Mulvey in 1975, focused on the objectification of women through depictions structured to gratify a male heterosexual perspective. In this chapter we will revisit this concept and investigate how that gaze may have shifted away from a primarily heterosexual perspective to a socially dominant male perspective (maleness here referring to dominance rather than specific gender, just as “whiteness” might refer to privilege rather than race). With gender roles in an increasingly global and mobile society becoming more fluid and complex, opening up visibility to LGBTQ communities, …

Sketch-Plan Book: A Teacher’S Planning Resource For The Secondary Classroom, Katherine M. Avra

Theses and Dissertations--Art and Visual Studies

Teacher planning is a necessary process by which educators establish, facilitate, monitor, and evaluate lessons and learning within their classroom. For art educators, sketchbooks have been a foundational pillar in art curricula. The sketchbook has a lengthy legacy of yielding common, structured assignments. However, recent considerations have reframed sketchbook practices. Contemporary analysis has produced a pedagogical shift in approaches to sketchbooks and planning for sketchbook inclusion in the classroom. The sketch-plan book offers art teachers a streamlined resource to collect and maintain lesson ideas and inclusions, track on-going lessons, and plan for future teaching and learning. The purpose of this …

In Black And White: Richmond’S Monument Avenue Recontextualized Through The Photographic Archive, Charlsa Anne Hensley

Theses and Dissertations--Art and Visual Studies

The release of the Monument Avenue Commission Report in July, 2018 was the culmination of over one year of research and collaboration with community members of Richmond, Virginia on how the city should approach the contentious history of Monument Avenue’s five Confederate centerpieces. What the monuments have symbolized within the predominately rich, white neighborhood and outside of its confines has been a matter of debate ever since they were unveiled, but the recent publicity accorded to Confederate monuments has led to considerations by historians, city leaders, and the public regarding recontextualization of Confederate monuments.

Recontextualization of the monuments should not …