Digital Commons Network^™

Sensitive Research, Practice And Design In Hci, Stevie Chancellor, Nazanin Andalibi, Lindsay Blackwell, David Nemer, Wendy Moncur

Information Science Faculty Publications

New research areas in HCI examine complex and sensitive research areas, such as crisis, life transitions, and mental health. Further, research in complex topics such as harassment and graphic content can leave researchers vulnerable to emotional and physical harm. There is a need to bring researchers together to discuss challenges across sensitive research spaces and environments. We propose a workshop to explore the methodological, ethical, and emotional challenges of sensitive research in HCI. We will actively recruit from diverse research environments (industry, academia, government, etc.) and methods areas (qualitative, quantitative, design practices, etc.) and identify commonalities in and encourage relationship-building …

Discovery Of Glycerol Phosphate Modification On Streptococcal Rhamnose Polysaccharides., Rebecca J Edgar, Vincent P Van Hensbergen, Alessandro Ruda, Andrew G Turner, Pan Deng, Yoann Le Breton, Najib M El-Sayed, Ashton T Belew, Kevin S Mciver, Alastair G Mcewan, Andrew J Morris, Gérard Lambeau, Mark J Walker, Jeffrey S. Rush, Konstantin V Korotkov, Göran Widmalm, Nina M Van Sorge, Natalia Korotkova

Molecular and Cellular Biochemistry Faculty Publications

Cell wall glycopolymers on the surface of Gram-positive bacteria are fundamental to bacterial physiology and infection biology. Here we identify gacH, a gene in the Streptococcus pyogenes group A carbohydrate (GAC) biosynthetic cluster, in two independent transposon library screens for its ability to confer resistance to zinc and susceptibility to the bactericidal enzyme human group IIA-secreted phospholipase A

Design Optimization Of Electric Machines With 3d Fea And A New Hybrid Doe-De Numerical Algorithm, Narges Taran, Vandana Rallabandi, Dan M. Ionel, Greg Heins, Dean Patterson, Ping Zhou

Power and Energy Institute of Kentucky Faculty Publications

This paper discusses the multi-objective optimization of axial flux permanent magnet (AFPM) machines with ferrite spoke-type magnets, utilizing 3D finite element models. Three-dimensional finite element analysis is computationally expensive, and furthermore, substantial computation time is expended by optimization algorithms in evaluating low performing designs whose performance is far from the optimum if the search space is not specified correctly. In this regard, this work proposes two new methods for identifying the search space. The search is limited to ranges of input geometric variables where high performing designs are likely to be found. The optimization algorithm utilized is based on surrogate …

Inductance Testing For Ipm Synchronous Machines According To The New Ieee Std 1812 And Typical Laboratory Practices, Vandana Rallabandi, Narges Taran, Dan M. Ionel, Ping Zhou

Power and Energy Institute of Kentucky Faculty Publications

Equivalent circuit parameters serve as the basis for performance estimation and implementation of power electronic drives controls and therefore their accurate evaluation is very important. Specified in the newly approved IEEE Std 1812, a short-circuit test can be employed, in combination with an open-circuit measurement, in order to determine the back emf and the synchronous inductance. In the case of interior permanent magnet (IPM) machines, this approach can be used only to determine the d-axis inductance and additional and separate measurements are required for the q-axis inductance. In this respect, various methods, inclusive of dc step response tests, on-load tests, …

A Comparative Study Of Methods For Calculating Ac Winding Losses In Permanent Magnet Machines, Narges Taran, Vandana Rallabandi, Dan M. Ionel, Greg Heins, Dean Patterson

Power and Energy Institute of Kentucky Faculty Publications

In this study different methods of estimating the additional ac winding loss due to eddy currents at open-circuit are explored. A comparative study of 2D and 3D FEA, and hybrid numerical and analytical methods is performed in order to recommend feasible approaches to be employed. Axial flux permanent magnet (AFPM) machine case studies are included, namely a machine with open slots and a coreless configuration. These machine topologies are expected to present a substantial amount of ac winding loss, which would therefore need to be considered during optimal design. This study is one of the first ones to compare meticulous …

Development And Evaluation Of A One-Step Multiplex Real-Time Taqman® Rt-Qpcr Assay For The Detection And Genotyping Of Equine G3 And G14 Rotaviruses In Fecal Samples, Mariano Carossino, Maria E. Barrandeguy, Erdal Erol, Yanqiu Li, Udeni B. R. Balasuriya

Veterinary Science Faculty Publications

Background: Equine rotavirus A (ERVA) is the leading cause of diarrhea in neonatal foals and has a negative impact on equine breeding enterprises worldwide. Among ERVA strains infecting foals, the genotypes G3P[12] and G14P[12] are the most prevalent, while infections by strains with other genomic arrangements are infrequent. The identification of circulating strains of ERVA is critical for diagnostic and surveillance purposes, as well as to understand their molecular epidemiology. Current genotyping methods available for ERVA and rotaviruses affecting other animal species rely on Sanger sequencing and are significantly time-consuming, costly and labor intensive. Here, we developed the first one-step …

The Effects Of A Bacterial Endotoxin On Behavior And Sensory-Cns-Motor Circuits In Drosophila Melanogaster, Oscar Istas, Abigail Greenhalgh, Robin L. Cooper

Biology Faculty Publications

The effect of bacterial sepsis on animal behavior and physiology is complex due to direct and indirect actions. The most common form of bacterial sepsis in humans is from gram-negative bacterial strains. The endotoxin (lipopolysaccharide, LPS) and/or associated peptidoglycans from the bacteria are the key agents to induce an immune response, which then produces a cascade of immunological consequences. However, there are direct actions of LPS and associated peptidoglycans on cells which are commonly overlooked. This study showed behavioral and neural changes in larval Drosophila fed commercially obtained LPS from Serratia marcescens. Locomotor behavior was not altered, but feeding …

Trans-Cinnamic Acid-Induced Leaf Expansion Involves An Auxin-Independent Component, Jasmina Kurepa, Jan A. Smalle

Plant and Soil Sciences Faculty Publications

The phenylpropanoid pathway, the source of a large array of compounds with diverse functions, starts with the synthesis of trans-cinnamic acid (t-CA) that is converted by cinnamate-4-hydroxylase (C4H) into p-coumaric acid. We have recently shown that in Arabidopsis, exogenous t-CA promotes leaf growth by increasing cell expansion and that this response requires auxin signaling. We have also shown that cell expansion is increased in C4H loss-of-function mutants. Here we provide further evidence that leaf growth is enhanced by either t-CA or a t-CA derivative that accumulates upstream of C4H. We also show that …

Bovine Milk Extracellular Vesicles (Evs) Modification Elicits Skeletal Muscle Growth In Rats, Hailey A. Parry, Christopher Brooks Mobley, Petey W. Mumford, Matthew A. Romero, Cody T. Haun, Yufeng Zhang, Paul A. Roberson, Janos Zempleni, Arny A. Ferrando, Ivan J. Vechetti Jr., John J. Mccarthy, Kaelin C. Young, Michael D. Roberts, Andreas N. Kavazis

Physiology Faculty Publications

The current study investigated how bovine milk extracellular vesicles (EVs) affected rotarod performance and biomarkers of skeletal muscle physiology in young, growing rats. Twenty-eight-day Fisher 344 rats were provided an AIN-93G-based diet for 4 weeks that either remained unadulterated [EVs and RNA-sufficient (ERS; n = 12)] or was sonicated [EVs and RNA-depleted (ERD; n = 12)]. Prior to (PRE) and on the last day of the intervention (POST), animals were tested for maximal rotarod performance. Following the feeding period, the gastrocnemius muscle was analyzed at the histological, biochemical, and molecular levels and was also used to measure mitochondrial function and …

Eis Inhibitors, Sylvie Garneau-Tsodikova, Oleg V. Tsodikov

Pharmaceutical Sciences Faculty Patents

Compounds and compositions are disclosed, which are useful as inhibitors of acetyltransferase Eis, a mediator of kanamycin resistance in Mycobacterium tuberculosis.

Surface-Controlled Dissolution Rates: A Case Study Of Nanoceria In Carboxylic Acid Solutions, Eric A. Grulke, Matthew J. Beck, Robert A. Yokel, Jason M. Unrine, Uschi M. Graham, Matthew L. Hancock

Chemical and Materials Engineering Faculty Publications

Nanoparticle dissolution in local milieu can affect their ecotoxicity and therapeutic applications. For example, carboxylic acid release from plant roots can solubilize nanoceria in the rhizosphere, affecting cerium uptake in plants. Nanoparticle dispersions were dialyzed against ten carboxylic acid solutions for up to 30 weeks; the membrane passed cerium-ligand complexes but not nanoceria. Dispersion and solution samples were analyzed for cerium by inductively coupled plasma mass spectrometry (ICP-MS). Particle size and shape distributions were measured by transmission electron microscopy (TEM). Nanoceria dissolved in all carboxylic acid solutions, leading to cascades of progressively smaller nanoparticles and producing soluble products. The dissolution …

Novel Calibrated Short Tr Recovery (Castrr) Method For Brain-Blood Partition Coefficient Correction Enhances Gray-White Matter Contrast In Blood Flow Measurements In Mice, Scott W. Thalman, David K. Powell, Ai-Ling Lin

Biomedical Engineering Faculty Publications

The goal of the study was to develop a novel, rapid Calibrated Short TR Recovery (CaSTRR) method to measure the brain-blood partition coefficient (BBPC) in mice. The BBPC is necessary for quantifying cerebral blood flow (CBF) using tracer-based techniques like arterial spin labeling (ASL), but previous techniques required prohibitively long acquisition times so a constant BBPC equal to 0.9 mL/g is typically used regardless of studied species, condition, or disease. An accelerated method of BBPC correction could improve regional specificity in CBF maps particularly in white matter. Male C57Bl/6N mice (n = 8) were scanned at 7T using CaSTRR …

An Examination Of Factors That Influence Social Networking Community Participation Among Millennials, Celeste See Pui Ng, Anita Lee-Post

Marketing & Supply Chain Faculty Publications

This study investigates main and moderating factors that influence Millennials' intention to participate in a social networking community (SNC). The authors modified the unified theory of consumers' acceptance and use of technology (UTAUT2) to incorporate six main and two moderating factors to explain Millennials' SNC participation intention. By considering the implications of the unique characteristics of Millennials on their social networking behavior, the authors' model is better suited to answer what drives these tech-savvy individuals to participate in a SNC via such sites as Facebook. Specifically, the authors find that hedonic motivation, trust in technology, trust in community, and social …

Opposing Abortion To Protect Women: Transnational Strategy Since The 1990s, Carol Mason

Gender and Women's Studies Faculty Publications

This article examines the transnational work that since the 1990s has increasingly opposed abortion in terms of protecting women. It therefore explores how pro-woman rhetoric is used to foster right-wing politics by way of, and beyond, the fight over abortion. Narratives depicting white women as dupes of a sordid, satanic system of abortion provision ignore the fact that most women report feeling relief—not grief, regret, or trauma—after terminating an unwanted pregnancy. To get a sense of the political and cultural influence that right-wing movements gain when they play the woman card, we must trace antiabortion collaborations transnationally. Reading representations of …

Rationale And Methods For A Multicenter Clinical Trial Assessing Exercise And Intensive Vascular Risk Reduction In Preventing Dementia (Rrad Study), Amanda N. Szabo-Reed, Eric Vidoni, Ellen F. Binder, Jeffrey Burns, C. Munro Cullum, William P. Gahan, Aditi Gupta, Linda S. Hynan, Diana R. Kerwin, Heidi Rossetti, Ann M. Stowe, Wanpen Vongpatanasin, David C. Zhu, Rong Zhang, Jeffrey N. Keller

Neurology Faculty Publications

Alzheimer's Disease (AD) is an age-related disease with modifiable risk factors such as hypertension, hypercholesterolemia, obesity, and physical inactivity influencing the onset and progression. There is however, no direct evidence that reducing these risk factors prevents or slows AD. The Risk Reduction for Alzheimer's Disease (rrAD) trial is designed to study the independent and combined effects of intensive pharmacological control of blood pressure and cholesterol and exercise training on neurocognitive function. Six hundred and forty cognitively normal older adults age 60 to 85 years with hypertension and increased risk for dementia will be enrolled. Participants are randomized into one of …

Investigation Of Factors That Influence Public Librarians’ Social Media Use For Marketing Purposes: An Adoption Of The Technology Acceptance Model And Theory Of Planned Behavior, Soohyung Joo, Namjoo Choi, Lindsey M. Harper

Information Science Faculty Publications

This study aims to explore multiple factors that are associated with social media use by public librarians for marketing purposes. Based on the technology acceptance model and theory of planned behavior, the effects of five factors—usefulness, ease of use, attitude, subjective norms, and behavioral control—on social media use intention were examined. A survey was conducted, and 462 valid responses were collected from public librarians across the United States. The findings revealed that all five factors have a significant impact on librarians’ intention to engage in social media activities for library marketing. Perceived behavioral control factors were the most influential on …

Time For A Fresh Look At Strict Liability For Pharmaceuticals, Mary J. Davis

Law Faculty Scholarly Articles

Part I provides a brief and basic explanation of pharmaceutical liability treatment. Part II explains the impact of federal preemption doctrine, which has dramatically limited the operation of tort law in pharmaceutical liability cases. Part III explains the parallel trends in the marketing and use of pharmaceuticals that increase the incidence of adverse drug events, affect prescribing practices, and fail to enhance informed practitioner and consumer choice in use of pharmaceuticals. Part IV provides support for the application of strict liability given the convergence of these trends. This Part also provides a theoretical justification for strict liability in tort for …

Evaluación De La Presencia De Mujeres En Gabinetes Subnacionales De Argentina (1992-2016), Tiffany D. Barnes, Tomás Ciocci, Débora Lopreite

Political Science Faculty Publications

¿Cuáles son los factores que explican la inclusión de las mujeres en puestos políticos subnacionales? La literatura convencional ha examinado las designaciones de mujeres en gabinetes nacionales, pero escasa atención se ha prestado a los gobiernos provinciales. Para abordar este vacío, adoptamos el enfoque de oferta y demanda. De este modo, utilizando una base de datos sobre las 24 jurisdicciones subnacionales argentinas desde 1992 a 2016, encontramos que los factores del lado de la demanda son más relevantes. Esto se explica, en primer término, ya que la probabilidad de que sean nombradas ministras aumenta junto al tamaño del gabinete, cuando …

Nutrition And Environmental Pollution Extension Curriculum Improved Diet-Related Behaviors And Environmental Health Literacy, Dawn Brewer, Hannah Bellamy, Anna Hoover, Annie Koempel, Lisa Gaetke

Dietetics and Human Nutrition Faculty Publications

Kentucky experiences some of the nation’s worst health outcomes related to obesity, diabetes, high blood pressure, and other age-related chronic diseases linked with oxidative stress and inflammation, which in turn are associated with poor diet, lack of physical activity, and exposure to certain environmental pollutants. In the Commonwealth, deteriorating infrastructure, inappropriate waste disposal, and potential occupational injury related to mining, agriculture, and other regionally important industries exacerbate the need for residents to have basic knowledge of potential environmental health threats. Unfortunately, community-level understanding of the complex connections between environmental exposures and health is limited, with many Kentuckians unaware that the …

The Flavonoid Metabolite 2,4,6-Trihydroxybenzoic Acid Is A Cdk Inhibitor And An Anti-Proliferative Agent: A Potential Role In Cancer Prevention, Ranjini Sankaranarayanan, Chaitanya K. Valiveti, Ramesh Kumar Dhandapani, Severine Van Slambrouck, Siddharth S. Kesharwani, Teresa Seefeldt, Joy Scaria, Hemachand Tummala, G. Jayarama Bhat

Entomology Faculty Publications

Flavonoids have emerged as promising compounds capable of preventing colorectal cancer (CRC) due to their anti-oxidant and anti-inflammatory properties. It is hypothesized that the metabolites of flavonoids are primarily responsible for the observed anti-cancer effects owing to the unstable nature of the parent compounds and their degradation by colonic microflora. In this study, we investigated the ability of one metabolite, 2,4,6-trihydroxybenzoic acid (2,4,6-THBA) to inhibit Cyclin Dependent Kinase (CDK) activity and cancer cell proliferation. Using in vitro kinase assays, we demonstrated that 2,4,6-THBA dose-dependently inhibited CDKs 1, 2 and 4 and in silico studies identified key amino acids involved in …

Poster Session 2, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• 2.1 Climate Change Impacts on Sediment Transport in a Lowland Watershed System: Controlling Processes and Projection
• 2.2 Assessment of MRSA Presence in Suburban WWTPs Effluent and Receiving Streams in Lexington, Kentucky
• 2.3 Heavy Metal Capture and Detection Using Colored Synthetic Dithiol
• 2.4 Quantifying Nutrient Fate and Transport in Karst Agroecosystems of Central Kentucky: Application of High-Resolution Sensors, Numerical Modeling, and Isotope Tracers
• 2.5 Magnetic Nanocomposite Materials for the Detection and Removal of Halogenated Organic Pollutants in Contaminated Water Sources
• 2.6 Measuring and Modeling Morphologic Processes in Karst to Sustain Water Resources in the Future
• 2.7 Impedance Spectroscopy Based Evaluation of …

Session 2a: Ecology, Biology, And Climate, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• Ecological Monitoring of WRP Easements in Western Kentucky
• Discussion of Factors Influencing Ecosystem Productivity in the Greenup Pool of the Ohio River
• Biotic Interactions Between a Bloom-Forming Cyanobacteria and a Planktonic Protist: Implications for Toxin Production
• The Use of Multiple Large and Detailed Databases to Evaluate the Impacts of Climate Change on Water Resources in the Ohio River Basin
• Evaluating and Addressing Climate Awareness and Water in Kentucky

Poster Session 1, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• 1.1 Evaluating Soil Characteristics in Rural and Urban Areas
• 1.2 Use of eDNA in Detection of Multiple Salamander Species in Eastern Kentucky Streams
• 1.3 Evaluation of biomass and filtration method on eDNA detection of fantail darters (Etheostoma flabellare)
• 1.4 The Impact of Agricultural Development on Nutrient Contamination Hotspots Within a Small, Intermittent Watershed at EKU’s Meadowbrook Farm, Madison County, Kentucky
• 1.5 How Does Drought Length Impact the Runoff and Nutrient Storm Response in an Agricultural, Intermittent Catchment in Central Kentucky?
• 1.6 Analyzing Sanitary Sewer Overflow Sensitivity to Storm Characteristics
• 1.7 Evaluating Soil Properties in No-Till and Conventional Tillage Systems
• 1.8 …

Proceedings Of 2019 Kentucky Water Resources Annual Symposium, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

This symposium was planned and conducted as a part of the state water resources research institute annual program that is supported by Grant/Cooperative Agreement Number G16AP00055 from the United States Geological Survey. The contents of this proceedings document and the views and conclusions presented at the symposium are solely the responsibility of the individual authors and presenters and do not necessarily represent the official views of the USGS or of the symposium organizers and sponsors. This publication is produced with the understanding that the United States Government is authorized to reproduce and distribute reprints for government purposes. Mention of trade …

Polymer Gels With Tunable Ionic Seebeck Coefficient For Ultra-Sensitive Printed Thermopiles, Dan Zhao, Anna Martinelli, Andreas Willfahrt, Thomas Fischer, Diana Bernin, Zia Ullah Khan, Maryam Shahi, Joseph W. Brill, Magnus P. Jonsson, Simone Fabiano, Xavier Crispin

Physics and Astronomy Faculty Publications

Measuring temperature and heat flux is important for regulating any physical, chemical, and biological processes. Traditional thermopiles can provide accurate and stable temperature reading but they are based on brittle inorganic materials with low Seebeck coefficient, and are difficult to manufacture over large areas. Recently, polymer electrolytes have been proposed for thermoelectric applications because of their giant ionic Seebeck coefficient, high flexibility and ease of manufacturing. However, the materials reported to date have positive Seebeck coefficients, hampering the design of ultra-sensitive ionic thermopiles. Here we report an “ambipolar” ionic polymer gel with giant negative ionic Seebeck coefficient. The latter can …

A Systematic Study On The Effects Of Dimensional And Materials Tolerances On Permanent Magnet Synchronous Machines Based On The Ieee Std 1812, Narges Taran, Vandana Rallabandi, Dan M. Ionel, Ping Zhou, Mark Theile, Greg Heins

Power and Energy Institute of Kentucky Faculty Publications

In the process of designing and manufacturing an electrical machine, a systematic study of dimensional and material tolerances is of the utmost importance. This paper proposes a systematic method by which the effect of design specification variations on permanent magnet (PM) synchronous machine performance may be identified and quantified. The method combines design of experiments techniques, open-circuit and short-circuit physical measurements, and virtual test simulations conducted based on the recently approved IEEE Std 1812 testing guide. Three case studies, two provided by a spoke-type PM radial field machine configuration, in two designs with different electromagnetic loading, and an axial flux …

A Pilot Study Identifying Brain-Targeting Adaptive Immunity In Pediatric Extracorporeal Membrane Oxygenation Patients With Acquired Brain Injury, Sterling B. Ortega, Poornima Pandiyan, Jana Windsor, Vanessa O. Torres, Uma M. Selvaraj, Amy Lee, Michael Morriss, Fenghua Tian, Lakshmi Raman, Ann M. Stowe

Neurology Faculty Publications

OBJECTIVES: Extracorporeal membrane oxygenation provides short-term cardiopulmonary life support, but is associated with peripheral innate inflammation, disruptions in cerebral autoregulation, and acquired brain injury. We tested the hypothesis that extracorporeal membrane oxygenation also induces CNS-directed adaptive immune responses which may exacerbate extracorporeal membrane oxygenation-associated brain injury.

DESIGN: A single center prospective observational study.

SETTING: Pediatric and cardiac ICUs at a single tertiary care, academic center.

PATIENTS: Twenty pediatric extracorporeal membrane oxygenation patients (0-14 yr; 13 females, 7 males) and five nonextracorporeal membrane oxygenation Pediatric Logistic Organ Dysfunction score matched patients.

INTERVENTIONS: None.

MEASUREMENTS AND MAIN RESULTS: Venous blood samples were …

Network Analysis Approaches To Collaborative Information Seeking In Inter-Professional Health Care Teams, J. David Johnson

Communication Faculty Publications

Introduction: It is widely believed that interprofessional health care teams can improve patient care and increase safety; however, intra-team communication has often proven to be problematic. One particularly important aspect of this problem is how teams collaborate to seek the information needed to address the issues they are confronting. Network analysis offers a rich set of concepts for tackling this problem.

Method: Review essay.

Analysis and results: Network analysis approaches offer the promise of more nuanced approaches and diagnostic tools that could address these issues by focusing on its elements: reciprocity, centrality, density, fault lines, multi-team systems, …

Saturated Fatty Acid Activates T Cell Inflammation Through A Nicotinamide Nucleotide Transhydrogenase (Nnt)-Dependent Mechanism, Grace Mccambridge, Madhur Agrawal, Alanna Keady, Philip A. Kern, Hatice Hasturk, Barbara S. Nikolajczyk, Leena P. Bharath

Pharmacology and Nutritional Sciences Faculty Publications

Circulating fatty acids (FAs) increase with obesity and can drive mitochondrial damage and inflammation. Nicotinamide nucleotide transhydrogenase (NNT) is a mitochondrial protein that positively regulates nicotinamide adenine dinucleotide phosphate (NADPH), a key mediator of energy transduction and redox homeostasis. The role that NNT-regulated bioenergetics play in the inflammatory response of immune cells in obesity is untested. Our objective was to determine how free fatty acids (FFAs) regulate inflammation through impacts on mitochondria and redox homeostasis of peripheral blood mononuclear cells (PBMCs). PBMCs from lean subjects were activated with a T cell-specific stimulus in the presence or absence of generally pro-inflammatory …

Spore Forming Actinobacterial Diversity Of Cholistan Desert Pakistan: Polyphasic Taxonomy, Antimicrobial Potential And Chemical Profiling, Adeela Fatima, Usman Aftab, Khaled A. Shaaban, Jon S. Thorson, Imran Sajid

Center for Pharmaceutical Research and Innovation Faculty Publications

Background: Actinobacteria are famous for the production of unique secondary metabolites that help in controlling the continuously emerging drug resistance all over the globe. This study aimed at the investigation of an extreme environment the Cholistan desert, located in southern Punjab, Pakistan, for actinobacterial diversity and their activity against methicillin resistant Staphylococcus aureus (MRSA). The Cholistan desert is a sub-tropical and arid ecosystem with harsh environment, limited rainfall and low humidity. The 20 soil and sand samples were collected from different locations in the desert and the actinobacterial strains were selectively isolated. The isolated strains were identified using a polyphasic …